AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1212170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212170 MtrunA17_Chr4g0060981 95.890 219 9 0 1 219 1 219 9.62e-149 412
Msa1212170 MtrunA17_Chr5g0406031 40.404 198 111 2 25 219 41 234 9.26e-52 167
Msa1212170 MtrunA17_Chr8g0371291 38.095 210 123 3 8 217 21 223 2.03e-46 153
Msa1212170 MtrunA17_Chr5g0444161 35.437 206 114 6 17 219 41 230 5.37e-27 103
Msa1212170 MtrunA17_Chr5g0444141 35.533 197 112 6 27 219 80 265 8.38e-26 101
Msa1212170 MtrunA17_Chr6g0451051 46.575 73 38 1 148 219 39 111 5.26e-16 72.4
Msa1212170 MtrunA17_Chr3g0128771 33.333 93 59 1 130 219 72 164 2.64e-15 71.2
Msa1212170 MtrunA17_Chr5g0437361 45.833 72 36 1 148 216 74 145 4.63e-15 70.1
Msa1212170 MtrunA17_Chr8g0383941 36.735 98 52 2 122 217 88 177 1.14e-14 70.1
Msa1212170 MtrunA17_Chr8g0383881 43.056 72 39 1 148 217 102 173 3.44e-14 68.9
Msa1212170 MtrunA17_Chr8g0383861 43.056 72 39 1 148 217 112 183 3.83e-14 68.9
Msa1212170 MtrunA17_Chr8g0388231 39.726 73 41 1 149 218 309 381 5.63e-14 70.1
Msa1212170 MtrunA17_Chr7g0246761 55.319 47 21 0 170 216 86 132 5.69e-14 67.8
Msa1212170 MtrunA17_Chr6g0451031 41.667 72 42 0 148 219 43 114 8.33e-14 65.9
Msa1212170 MtrunA17_Chr3g0118071 32.456 114 66 3 106 216 41 146 8.53e-14 67.8
Msa1212170 MtrunA17_Chr3g0120011 38.961 77 45 1 142 216 85 161 1.02e-13 67.4
Msa1212170 MtrunA17_Chr5g0430781 40.000 80 44 2 139 216 75 152 1.40e-13 67.4
Msa1212170 MtrunA17_Chr3g0143301 46.667 75 38 2 145 219 44 116 1.64e-13 65.1
Msa1212170 MtrunA17_Chr7g0274721 36.620 71 45 0 146 216 65 135 1.69e-13 66.2
Msa1212170 MtrunA17_Chr8g0383871 41.667 72 40 1 148 217 111 182 2.35e-13 66.6
Msa1212170 MtrunA17_Chr8g0383921 41.667 72 40 1 148 217 110 181 2.45e-13 66.6
Msa1212170 MtrunA17_Chr7g0244851 41.892 74 38 2 149 219 81 152 4.30e-13 65.1
Msa1212170 MtrunA17_Chr8g0370171 41.333 75 41 1 145 216 66 140 4.98e-13 65.5
Msa1212170 MtrunA17_Chr4g0030831 40.541 74 41 1 149 219 84 157 5.70e-13 64.7
Msa1212170 MtrunA17_Chr6g0486651 43.836 73 38 2 149 219 93 164 5.98e-13 67.0
Msa1212170 MtrunA17_Chr2g0327791 44.118 68 35 1 155 219 113 180 6.17e-13 65.5
Msa1212170 MtrunA17_Chr5g0407431 42.466 73 39 1 148 217 76 148 6.19e-13 65.5
Msa1212170 MtrunA17_Chr8g0383931 50.000 52 26 0 166 217 133 184 7.37e-13 65.5
Msa1212170 MtrunA17_Chr8g0383901 50.000 52 26 0 166 217 123 174 7.41e-13 65.1
Msa1212170 MtrunA17_Chr6g0451041 55.319 47 21 0 173 219 17 63 7.65e-13 62.4
Msa1212170 MtrunA17_Chr7g0248831 38.356 73 42 1 149 218 80 152 9.64e-13 64.3
Msa1212170 MtrunA17_Chr8g0348151 40.000 70 41 1 149 218 90 158 1.15e-12 64.3
Msa1212170 MtrunA17_Chr3g0086801 41.333 75 43 1 145 219 76 149 1.20e-12 63.5
Msa1212170 MtrunA17_Chr2g0295571 39.726 73 43 1 148 219 92 164 1.49e-12 65.5
Msa1212170 MtrunA17_Chr1g0157091 45.070 71 37 2 149 219 48 116 1.57e-12 62.4
Msa1212170 MtrunA17_Chr7g0248841 51.064 47 23 0 171 217 101 147 1.58e-12 63.2
Msa1212170 MtrunA17_Chr5g0426871 38.667 75 42 3 149 219 108 182 2.10e-12 65.5
Msa1212170 MtrunA17_Chr3g0113811 42.105 76 39 2 146 216 73 148 2.33e-12 63.2
Msa1212170 MtrunA17_Chr4g0067591 37.662 77 47 1 143 219 74 149 2.85e-12 64.3
Msa1212170 MtrunA17_Chr3g0118061 31.250 112 67 4 115 216 35 146 3.17e-12 63.5
Msa1212170 MtrunA17_Chr3g0129671 40.845 71 40 1 149 217 109 179 3.38e-12 63.5
Msa1212170 MtrunA17_Chr1g0184631 38.158 76 43 2 146 217 65 140 3.61e-12 62.8
Msa1212170 MtrunA17_Chr7g0274711 38.158 76 43 2 146 217 64 139 3.81e-12 62.4
Msa1212170 MtrunA17_Chr3g0105441 36.364 77 44 1 148 219 101 177 4.03e-12 63.9
Msa1212170 MtrunA17_Chr2g0307411 40.278 72 41 1 148 219 76 145 5.01e-12 62.4
Msa1212170 MtrunA17_Chr1g0167811 39.241 79 38 3 148 219 93 168 5.34e-12 63.9
Msa1212170 MtrunA17_Chr1g0191601 42.373 59 30 1 161 219 118 172 5.67e-12 63.9
Msa1212170 MtrunA17_Chr7g0274741 38.356 73 41 2 148 216 69 141 6.49e-12 62.0
Msa1212170 MtrunA17_Chr5g0441191 35.714 70 44 1 147 216 173 241 7.33e-12 63.5
Msa1212170 MtrunA17_Chr7g0274731 35.211 71 46 0 146 216 86 156 7.53e-12 62.0
Msa1212170 MtrunA17_Chr2g0325141 50.000 46 23 0 174 219 91 136 7.76e-12 60.8
Msa1212170 MtrunA17_Chr7g0273051 47.826 46 24 0 174 219 120 165 9.71e-12 63.5
Msa1212170 MtrunA17_Chr3g0132491 39.474 76 42 2 148 219 106 181 1.11e-11 63.2
Msa1212170 MtrunA17_Chr4g0013561 35.294 68 44 0 149 216 85 152 1.16e-11 62.0
Msa1212170 MtrunA17_Chr5g0409211 41.791 67 33 1 156 216 70 136 1.17e-11 61.2
Msa1212170 MtrunA17_Chr7g0276591 38.710 62 38 0 155 216 82 143 1.18e-11 62.8
Msa1212170 MtrunA17_Chr3g0144241 38.571 70 43 0 148 217 78 147 1.20e-11 61.6
Msa1212170 MtrunA17_Chr1g0198201 38.028 71 42 1 148 216 60 130 1.25e-11 62.0
Msa1212170 MtrunA17_Chr8g0345361 43.056 72 39 2 149 219 92 162 1.30e-11 62.4
Msa1212170 MtrunA17_Chr4g0073131 42.424 66 37 1 155 219 82 147 1.46e-11 60.8
Msa1212170 MtrunA17_Chr1g0184171 37.333 75 43 2 148 219 102 175 1.48e-11 62.8
Msa1212170 MtrunA17_Chr8g0366561 50.000 48 24 0 172 219 140 187 1.51e-11 62.8
Msa1212170 MtrunA17_Chr1g0171791 36.986 73 45 1 147 219 132 203 1.65e-11 62.0
Msa1212170 MtrunA17_Chr5g0409201 40.000 70 36 1 155 218 68 137 2.00e-11 60.1
Msa1212170 MtrunA17_Chr3g0117821 51.163 43 21 0 174 216 85 127 2.04e-11 60.1
Msa1212170 MtrunA17_Chr8g0388201 29.167 96 65 1 125 217 295 390 2.35e-11 62.4
Msa1212170 MtrunA17_Chr1g0169651 39.437 71 40 2 149 219 660 727 2.75e-11 62.4
Msa1212170 MtrunA17_Chr8g0369571 48.889 45 23 0 174 218 90 134 2.76e-11 60.1
Msa1212170 MtrunA17_Chr3g0126611 32.184 87 55 2 133 216 288 373 2.88e-11 62.0
Msa1212170 MtrunA17_Chr4g0073141 32.632 95 53 2 136 219 95 189 3.25e-11 62.0
Msa1212170 MtrunA17_Chr4g0027761 39.130 69 40 1 148 216 99 165 3.34e-11 60.5
Msa1212170 MtrunA17_Chr4g0052671 50.000 44 22 0 174 217 106 149 3.39e-11 59.3
Msa1212170 MtrunA17_Chr6g0470751 36.842 76 44 2 148 219 96 171 3.92e-11 61.2
Msa1212170 MtrunA17_Chr2g0325161 50.000 46 23 0 174 219 123 168 3.97e-11 60.8
Msa1212170 MtrunA17_Chr3g0133001 52.174 46 22 0 174 219 156 201 4.12e-11 61.6
Msa1212170 MtrunA17_Chr8g0350741 51.111 45 22 0 172 216 87 131 4.21e-11 59.7
Msa1212170 MtrunA17_Chr2g0279431 48.936 47 24 0 170 216 157 203 4.41e-11 61.6
Msa1212170 MtrunA17_Chr2g0300331 48.077 52 27 0 168 219 163 214 4.55e-11 60.5
Msa1212170 MtrunA17_Chr5g0427431 52.174 46 22 0 174 219 160 205 4.88e-11 61.6
Msa1212170 MtrunA17_Chr3g0126931 28.571 147 86 4 89 216 23 169 4.89e-11 61.2
Msa1212170 MtrunA17_Chr2g0297011 34.722 72 46 1 149 219 76 147 6.93e-11 58.9
Msa1212170 MtrunA17_Chr5g0396601 38.889 72 41 2 149 217 322 393 9.58e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1212170 AT1G74410.1 56.373 204 84 2 17 219 22 221 1.26e-72 220
Msa1212170 AT5G66070.5 41.063 207 116 4 14 219 20 221 3.47e-49 160
Msa1212170 AT5G66070.1 41.063 207 116 4 14 219 20 221 3.47e-49 160
Msa1212170 AT5G66070.2 37.719 228 118 4 14 219 20 245 8.94e-47 155
Msa1212170 AT2G17730.1 38.462 208 120 4 16 219 38 241 1.33e-45 152
Msa1212170 AT4G35840.1 36.667 210 120 3 19 219 31 236 7.86e-44 147
Msa1212170 AT2G17730.2 38.191 199 115 4 16 210 38 232 2.13e-40 139
Msa1212170 AT4G35840.2 31.897 232 116 3 25 219 42 268 4.48e-37 130
Msa1212170 AT3G20395.1 29.825 228 134 5 5 219 2 216 1.26e-29 110
Msa1212170 AT5G66070.4 36.527 167 100 3 14 179 20 181 1.11e-26 101
Msa1212170 AT5G66070.3 33.511 188 101 4 14 179 20 205 2.28e-24 96.3
Msa1212170 AT3G10910.1 40.816 98 49 3 125 219 68 159 1.59e-16 74.7
Msa1212170 AT5G05280.1 41.667 72 42 0 148 219 87 158 1.66e-15 72.0
Msa1212170 AT2G27940.1 42.254 71 41 0 148 218 114 184 3.31e-15 72.4
Msa1212170 AT2G17450.1 44.872 78 35 2 148 217 68 145 1.97e-14 69.3
Msa1212170 AT5G01880.1 44.444 72 39 1 149 219 79 150 2.54e-14 68.6
Msa1212170 AT3G16720.1 38.961 77 47 0 143 219 88 164 6.62e-14 69.7
Msa1212170 AT4G10160.1 39.706 68 41 0 149 216 72 139 1.68e-13 67.4
Msa1212170 AT5G53110.1 37.363 91 51 2 131 218 287 374 2.02e-13 68.9
Msa1212170 AT5G42200.1 42.466 73 41 1 145 216 74 146 2.40e-13 65.9
Msa1212170 AT4G10150.1 38.235 68 42 0 149 216 86 153 4.62e-13 66.6
Msa1212170 AT4G17905.2 42.857 63 34 1 157 219 166 226 8.97e-13 66.6
Msa1212170 AT4G17905.1 42.857 63 34 1 157 219 140 200 9.01e-13 66.6
Msa1212170 AT4G09120.1 37.374 99 51 4 125 219 77 168 9.98e-13 66.6
Msa1212170 AT2G46160.1 39.241 79 39 1 149 218 104 182 1.13e-12 65.1
Msa1212170 AT2G46160.2 39.241 79 39 1 149 218 104 182 1.13e-12 65.1
Msa1212170 AT5G57750.1 37.879 66 39 1 155 218 101 166 1.16e-12 65.1
Msa1212170 AT1G20823.1 41.667 72 40 1 148 217 83 154 1.23e-12 64.7
Msa1212170 AT1G76410.1 35.165 91 57 1 129 217 57 147 1.63e-12 63.9
Msa1212170 AT1G35330.1 42.466 73 38 2 148 216 99 171 2.88e-12 65.1
Msa1212170 AT2G35910.1 40.580 69 40 1 149 216 121 189 3.48e-12 63.9
Msa1212170 AT2G42360.1 41.892 74 41 1 148 219 80 153 4.00e-12 63.9
Msa1212170 AT3G14320.1 52.174 46 22 0 174 219 88 133 4.90e-12 63.2
Msa1212170 AT4G40070.1 47.059 51 27 0 169 219 119 169 5.11e-12 64.3
Msa1212170 AT3G18930.2 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1212170 AT3G18930.3 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1212170 AT3G18930.1 35.417 96 49 2 135 219 108 201 5.78e-12 64.7
Msa1212170 AT4G09130.1 38.710 93 50 4 131 219 76 165 5.84e-12 64.3
Msa1212170 AT3G18773.1 38.889 72 42 1 148 217 102 173 7.23e-12 62.8
Msa1212170 AT2G20030.1 39.726 73 41 2 149 219 98 169 7.47e-12 64.3
Msa1212170 AT5G10380.1 41.667 72 41 1 149 219 109 180 8.35e-12 63.5
Msa1212170 AT1G53820.1 50.000 46 23 0 174 219 120 165 9.22e-12 63.5
Msa1212170 AT1G49220.1 40.541 74 42 1 146 217 105 178 9.86e-12 63.2
Msa1212170 AT1G72310.1 37.333 75 47 0 145 219 98 172 1.11e-11 63.5
Msa1212170 AT2G42350.1 42.029 69 40 0 148 216 74 142 1.39e-11 62.0
Msa1212170 AT3G05200.1 52.174 46 22 0 174 219 128 173 1.48e-11 63.5
Msa1212170 AT4G30370.1 40.541 74 39 2 149 219 89 160 1.67e-11 61.2
Msa1212170 AT4G09100.1 34.615 78 47 1 146 219 53 130 1.82e-11 60.1
Msa1212170 AT4G28890.1 39.726 73 41 2 149 219 97 168 2.19e-11 62.8
Msa1212170 AT2G18670.1 39.726 73 42 1 149 219 83 155 2.36e-11 60.8
Msa1212170 AT2G40830.1 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212170 AT2G40830.3 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212170 AT2G40830.4 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212170 AT2G40830.2 32.432 74 48 2 147 219 162 234 2.50e-11 62.4
Msa1212170 AT1G33480.1 35.294 68 44 0 149 216 75 142 2.86e-11 61.6
Msa1212170 AT1G35625.1 38.889 72 40 3 150 218 207 277 3.32e-11 62.0
Msa1212170 AT1G28040.1 30.973 113 66 3 106 218 197 297 3.99e-11 61.6
Msa1212170 AT2G18650.1 35.616 73 45 1 149 219 99 171 4.00e-11 62.0
Msa1212170 AT3G48030.1 36.986 73 44 2 149 219 180 252 4.77e-11 61.6
Msa1212170 AT2G44578.1 42.308 52 30 0 168 219 64 115 4.77e-11 59.3
Msa1212170 AT4G30400.1 37.143 70 42 1 149 216 107 176 5.44e-11 62.0
Msa1212170 AT3G48030.2 36.986 73 44 2 149 219 102 174 5.56e-11 61.2
Msa1212170 AT1G53010.1 30.233 129 68 4 112 219 48 175 6.89e-11 59.3
Msa1212170 AT1G49200.1 38.961 77 39 2 146 217 106 179 7.58e-11 60.1
Msa1212170 AT5G17600.1 48.837 43 22 0 174 216 142 184 7.78e-11 61.2
Msa1212170 AT1G35630.1 41.667 72 38 3 150 218 207 277 9.07e-11 60.8
Msa1212170 AT2G25410.1 46.000 50 27 0 169 218 322 371 9.45e-11 60.8
Msa1212170 AT5G07040.1 35.616 73 44 1 149 218 66 138 9.91e-11 58.5

Find 78 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACCAAAATAGCATTAT+TGG 0.170704 8_1:+72032773 None:intergenic
GTTTCATTGGGTGCCATTGC+CGG 0.215022 8_1:-72034645 Msa1212170:CDS
CTAGTCCAATAATGCTATTT+TGG 0.245191 8_1:-72032778 Msa1212170:three_prime_UTR
ATTGTAGCTGGGGCCCTTAC+AGG 0.247414 8_1:-72034723 Msa1212170:intron
CAGCAGCTTATGATTGAAAA+TGG 0.286404 8_1:+72035513 None:intergenic
ACGAAACGAAATTCCCTTCT+TGG 0.308335 8_1:-72035422 Msa1212170:CDS
TGGAAGCTTCCCGTGCTTAT+TGG 0.315076 8_1:-72034599 Msa1212170:CDS
AAAGGCGCATAATAGAAATT+TGG 0.316639 8_1:+72035384 None:intergenic
AAAATTTGAAATAGCAAATC+AGG 0.318338 8_1:-72032710 Msa1212170:three_prime_UTR
TTTCATTGGGTGCCATTGCC+GGG 0.321224 8_1:-72034644 Msa1212170:CDS
TTCACGCGGCGCGTCTTCTA+TGG 0.328316 8_1:-72034559 Msa1212170:intron
CTCTGATTGGCTATCATTCA+TGG 0.356125 8_1:-72035455 Msa1212170:exon
TTCCATTGATTGATTGTTGA+CGG 0.369847 8_1:-72035586 Msa1212170:five_prime_UTR
CTGTGATATAATGATCATAA+AGG 0.370931 8_1:+72035641 None:intergenic
CGAAACGAAATTCCCTTCTT+GGG 0.382549 8_1:-72035421 Msa1212170:CDS
TTTCTGTGCAATATGTTTGC+AGG 0.383281 8_1:-72033227 Msa1212170:intron
TCCCCGGAGAAAACCACTTT+TGG 0.384936 8_1:+72034668 None:intergenic
TACACATTCTGTCTGCAAAT+AGG 0.395070 8_1:+72032951 None:intergenic
AATTTACAGGACATTGAAGT+TGG 0.400528 8_1:-72033059 Msa1212170:intron
TTTCTCCGGGGAGTTTCATT+GGG 0.418806 8_1:-72034657 Msa1212170:CDS
AGCTGCTGTTGCTGCACCTA+TGG 0.420837 8_1:-72035498 Msa1212170:five_prime_UTR
ACTTGATATGCGTGGACAAA+TGG 0.421325 8_1:-72032995 Msa1212170:CDS
AAAACATGATAACTGAGTAT+TGG 0.436148 8_1:+72032747 None:intergenic
CACCAGCTATAGCTCCTGTA+AGG 0.439321 8_1:+72034709 None:intergenic
TGAATCCCGTGACAATCCTC+TGG 0.451565 8_1:+72033303 None:intergenic
GAATAAGCTACCGCACCATA+TGG 0.453688 8_1:-72033278 Msa1212170:CDS
TGGCTATCATTCATGGAAAC+TGG 0.457921 8_1:-72035448 Msa1212170:exon
TTCCCGTGCTTATTGGTGTA+TGG 0.470182 8_1:-72034592 Msa1212170:CDS
ACGTTTCCACACTCTCTGAT+TGG 0.472939 8_1:-72035468 Msa1212170:five_prime_UTR
CGCATATCAAGTGAAAAGTA+TGG 0.479146 8_1:+72033006 None:intergenic
CATTGCCGGGGCTATACTCT+CGG 0.488812 8_1:-72034631 Msa1212170:CDS
TCATGCCCTTCGAAACCATA+TGG 0.493143 8_1:+72033263 None:intergenic
GCAGCTAAGGCTACCAAAAG+TGG 0.501253 8_1:-72034681 Msa1212170:CDS
GCCCTTACAGGAGCTATAGC+TGG 0.509484 8_1:-72034711 Msa1212170:CDS
ATTTCTCCATGAAGTGGGAG+GGG 0.523573 8_1:-72032844 Msa1212170:three_prime_UTR
TTTCTCCATGAAGTGGGAGG+GGG 0.525103 8_1:-72032843 Msa1212170:three_prime_UTR
TATACTCTCGGTGGAGGTGT+TGG 0.526459 8_1:-72034619 Msa1212170:CDS
TTGTAAGCAGTTAAAATAGC+AGG 0.527550 8_1:+72034271 None:intergenic
TAGAGGAACTTGTTCGTGGA+AGG 0.529453 8_1:-72034315 Msa1212170:CDS
TGCAGTGAGAGTTCCAGAAA+AGG 0.530821 8_1:+72035366 None:intergenic
AGCCGTCAACAATCAATCAA+TGG 0.532462 8_1:+72035584 None:intergenic
TTCATAGAGGAACTTGTTCG+TGG 0.535580 8_1:-72034319 Msa1212170:CDS
CTCCACCGAGAGTATAGCCC+CGG 0.540703 8_1:+72034626 None:intergenic
TCAATGGAAAATGAAAAGTA+AGG 0.543772 8_1:+72035600 None:intergenic
GACAAAACGAAAACCCAAGA+AGG 0.544046 8_1:+72035408 None:intergenic
TTATTTCTCCATGAAGTGGG+AGG 0.545078 8_1:-72032846 Msa1212170:three_prime_UTR
TATTGGTGTATGGAACAAAC+TGG 0.545201 8_1:-72034582 Msa1212170:CDS
ACCGCACCATATGGTTTCGA+AGG 0.553055 8_1:-72033269 Msa1212170:CDS
TATTTCTCCATGAAGTGGGA+GGG 0.558759 8_1:-72032845 Msa1212170:three_prime_UTR
TTCATTGGGTGCCATTGCCG+GGG 0.560934 8_1:-72034643 Msa1212170:CDS
TGGAAACGTTTATGAGCCAT+AGG 0.562502 8_1:+72035482 None:intergenic
CGAGAGTATAGCCCCGGCAA+TGG 0.563534 8_1:+72034632 None:intergenic
TTCCATACACCAATAAGCAC+GGG 0.567244 8_1:+72034590 None:intergenic
TTCGAAGGGCATGAAGGCAG+AGG 0.573221 8_1:-72033254 Msa1212170:CDS
TGATTGAAAATGGAAGGATA+AGG 0.574012 8_1:+72035523 None:intergenic
AGCTTATGATTGAAAATGGA+AGG 0.578222 8_1:+72035517 None:intergenic
AATCCAGGCAGATTTCATAG+AGG 0.579193 8_1:-72034332 Msa1212170:intron
GAAAATGAAAAGTAAGGTGT+TGG 0.587260 8_1:+72035606 None:intergenic
TGATAGCCAATCAGAGAGTG+TGG 0.589978 8_1:+72035462 None:intergenic
GTTCCATACACCAATAAGCA+CGG 0.597188 8_1:+72034589 None:intergenic
TATTGGACTAGATTACAGAA+TGG 0.600503 8_1:+72032790 None:intergenic
CGGGGCTATACTCTCGGTGG+AGG 0.602147 8_1:-72034625 Msa1212170:CDS
AAGGATAAGGAAGATGAAGC+TGG 0.608532 8_1:+72035536 None:intergenic
TATGGTTTCGAAGGGCATGA+AGG 0.614703 8_1:-72033260 Msa1212170:CDS
ACAAAACGAAAACCCAAGAA+GGG 0.623335 8_1:+72035409 None:intergenic
TGGCACCCAATGAAACTCCC+CGG 0.623545 8_1:+72034652 None:intergenic
CTATCATTCATGGAAACTGG+TGG 0.623614 8_1:-72035445 Msa1212170:exon
GTTGGAGTTGCTAATAACGC+TGG 0.623875 8_1:-72033364 Msa1212170:CDS
GAATCCCGTGACAATCCTCT+GGG 0.625719 8_1:+72033304 None:intergenic
ATCACCCAGAGGATTGTCAC+GGG 0.633383 8_1:-72033308 Msa1212170:CDS
ACCAGCTATAGCTCCTGTAA+GGG 0.637027 8_1:+72034710 None:intergenic
CCGCACCATATGGTTTCGAA+GGG 0.637296 8_1:-72033268 Msa1212170:CDS
CCCTTCGAAACCATATGGTG+CGG 0.643401 8_1:+72033268 None:intergenic
TATCACCCAGAGGATTGTCA+CGG 0.646030 8_1:-72033309 Msa1212170:CDS
TGCCGGGGCTATACTCTCGG+TGG 0.658573 8_1:-72034628 Msa1212170:CDS
ATGGAACAAACTGGTTCACG+CGG 0.720989 8_1:-72034573 Msa1212170:CDS
CATAGCTTAGTATCACCCAG+AGG 0.736019 8_1:-72033319 Msa1212170:CDS
TGAAAAGTATGGTCGCAACG+AGG 0.774791 8_1:+72033017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAAAACAAATTATAAA+TGG + chr8_1:72034764-72034783 None:intergenic 10.0%
!!! TTATTTTGTGTTAATATTTA+CGG - chr8_1:72035420-72035439 Msa1212170:CDS 10.0%
!!! TTTTTTTGTTTGATTTGAAT+TGG - chr8_1:72033124-72033143 Msa1212170:intron 15.0%
!!! TATGAAAGTTTAAGAATTTT+TGG - chr8_1:72033203-72033222 Msa1212170:intron 15.0%
!!! ATGAAAGTTTAAGAATTTTT+GGG - chr8_1:72033204-72033223 Msa1212170:intron 15.0%
!! TCAGAATTTAAATATGATAT+AGG + chr8_1:72034485-72034504 None:intergenic 15.0%
!!! TATTTTGTGTTAATATTTAC+GGG - chr8_1:72035421-72035440 Msa1212170:CDS 15.0%
!!! TGAAAGTTTAAGAATTTTTG+GGG - chr8_1:72033205-72033224 Msa1212170:intron 20.0%
!!! ATAATTTGTTTATCCAAACA+GGG + chr8_1:72033270-72033289 None:intergenic 20.0%
!!! AATAATTTGTTTATCCAAAC+AGG + chr8_1:72033271-72033290 None:intergenic 20.0%
!! TCAAATAAACTAATTCAAAC+AGG - chr8_1:72033528-72033547 Msa1212170:intron 20.0%
!!! ATTGGTTTAAAATTATGCAT+CGG + chr8_1:72033974-72033993 None:intergenic 20.0%
!!! AAATTCTGAAACTAAAAAAG+AGG - chr8_1:72034496-72034515 Msa1212170:intron 20.0%
!! TACTATGTGTTAAATTTGTA+TGG + chr8_1:72034610-72034629 None:intergenic 20.0%
!! ATACAAATTTAACACATAGT+AGG - chr8_1:72034609-72034628 Msa1212170:CDS 20.0%
!! ATTGAGTTATGTATATACAT+TGG - chr8_1:72034675-72034694 Msa1212170:CDS 20.0%
!! CATTGAGAAATATAATTTAC+AGG - chr8_1:72035267-72035286 Msa1212170:intron 20.0%
!!! AAAATTTGAAATAGCAAATC+AGG - chr8_1:72035629-72035648 Msa1212170:five_prime_UTR 20.0%
! CTGTGATATAATGATCATAA+AGG + chr8_1:72032701-72032720 None:intergenic 25.0%
! TCAATGGAAAATGAAAAGTA+AGG + chr8_1:72032742-72032761 None:intergenic 25.0%
! TTGCACAAATCAATTTATTC+TGG - chr8_1:72033091-72033110 Msa1212170:intron 25.0%
!! TAAAAGAATTGCTATTGACT+AGG + chr8_1:72033244-72033263 None:intergenic 25.0%
!! TAGTCAATAGCAATTCTTTT+AGG - chr8_1:72033243-72033262 Msa1212170:CDS 25.0%
!! AGTCAATAGCAATTCTTTTA+GGG - chr8_1:72033244-72033263 Msa1212170:CDS 25.0%
! GTTTATCATATAAGCACTTA+CGG - chr8_1:72033312-72033331 Msa1212170:CDS 25.0%
! TTTATCATATAAGCACTTAC+GGG - chr8_1:72033313-72033332 Msa1212170:CDS 25.0%
! GTTTATTTGAGCTTATCTAT+TGG + chr8_1:72033518-72033537 None:intergenic 25.0%
! AGCTTGAAATGCATATATAT+AGG - chr8_1:72033867-72033886 Msa1212170:intron 25.0%
! GGATTTAAATGTTACAGAAT+TGG + chr8_1:72033992-72034011 None:intergenic 25.0%
! ATTCTGTAACATTTAAATCC+AGG - chr8_1:72033992-72034011 Msa1212170:intron 25.0%
!!! TTTTAAAAGGCAAAAATTGG+GGG + chr8_1:72034124-72034143 None:intergenic 25.0%
!!! CTTTTAAAAGGCAAAAATTG+GGG + chr8_1:72034125-72034144 None:intergenic 25.0%
!!! GCTTTTAAAAGGCAAAAATT+GGG + chr8_1:72034126-72034145 None:intergenic 25.0%
!!! TGCTTTTAAAAGGCAAAAAT+TGG + chr8_1:72034127-72034146 None:intergenic 25.0%
!!! TAGTTTTATGAGCATCTTTT+GGG - chr8_1:72034352-72034371 Msa1212170:intron 25.0%
! ATCTTTAAATGACCTACATT+GGG + chr8_1:72034847-72034866 None:intergenic 25.0%
! AATCTTTAAATGACCTACAT+TGG + chr8_1:72034848-72034867 None:intergenic 25.0%
! TTTCAGGATTTGTGATTTAA+TGG - chr8_1:72035145-72035164 Msa1212170:intron 25.0%
! ATAGGGAAAATGAAAAATTG+TGG + chr8_1:72035204-72035223 None:intergenic 25.0%
! TATCATCCAAATCTCATATA+GGG + chr8_1:72035221-72035240 None:intergenic 25.0%
! ATATCATCCAAATCTCATAT+AGG + chr8_1:72035222-72035241 None:intergenic 25.0%
!!! TTTTTTATTTCTCCATGAAG+TGG - chr8_1:72035489-72035508 Msa1212170:five_prime_UTR 25.0%
!!! TTTTTATTTCTCCATGAAGT+GGG - chr8_1:72035490-72035509 Msa1212170:five_prime_UTR 25.0%
! TTCTACCAAAATAGCATTAT+TGG + chr8_1:72035569-72035588 None:intergenic 25.0%
! AAAACATGATAACTGAGTAT+TGG + chr8_1:72035595-72035614 None:intergenic 25.0%
GAAAATGAAAAGTAAGGTGT+TGG + chr8_1:72032736-72032755 None:intergenic 30.0%
! TTCCATTGATTGATTGTTGA+CGG - chr8_1:72032753-72032772 Msa1212170:three_prime_UTR 30.0%
TGATTGAAAATGGAAGGATA+AGG + chr8_1:72032819-72032838 None:intergenic 30.0%
AGCTTATGATTGAAAATGGA+AGG + chr8_1:72032825-72032844 None:intergenic 30.0%
! AAAGGCGCATAATAGAAATT+TGG + chr8_1:72032958-72032977 None:intergenic 30.0%
AGGATATTAGAGATTACCAA+AGG + chr8_1:72033006-72033025 None:intergenic 30.0%
AAATCATCATAGATGGTACA+AGG + chr8_1:72033026-72033045 None:intergenic 30.0%
TTGTACCATCTATGATGATT+TGG - chr8_1:72033025-72033044 Msa1212170:CDS 30.0%
AATTACGAAGCTTAAACATG+AGG - chr8_1:72033058-72033077 Msa1212170:intron 30.0%
ATTACGAAGCTTAAACATGA+GGG - chr8_1:72033059-72033078 Msa1212170:intron 30.0%
ATTGGTTGTAGTGAAATTCA+TGG - chr8_1:72033142-72033161 Msa1212170:intron 30.0%
TTGTAGTGAAATTCATGGTT+TGG - chr8_1:72033147-72033166 Msa1212170:intron 30.0%
!!! TTTAAGAATTTTTGGGGTGT+TGG - chr8_1:72033211-72033230 Msa1212170:intron 30.0%
!! GTTATTTCTATAAGCTCTTC+AGG + chr8_1:72033416-72033435 None:intergenic 30.0%
AAACTTATGAAAACAGCTTC+CGG + chr8_1:72033461-72033480 None:intergenic 30.0%
!! TTGTGTTTTTCAATTGTAGC+TGG - chr8_1:72033604-72033623 Msa1212170:intron 30.0%
!! TGTGTTTTTCAATTGTAGCT+GGG - chr8_1:72033605-72033624 Msa1212170:intron 30.0%
! TTCACCTTGATTTTTGTATG+TGG - chr8_1:72033832-72033851 Msa1212170:intron 30.0%
TTGTCCACATACAAAAATCA+AGG + chr8_1:72033839-72033858 None:intergenic 30.0%
TAAGATGCATCTTAAAAACC+AGG + chr8_1:72033925-72033944 None:intergenic 30.0%
!!! TTAAGAGTGTGTTTTTTGAG+TGG - chr8_1:72033942-72033961 Msa1212170:intron 30.0%
TTAAAATTATGCATCGGAGA+TGG + chr8_1:72033968-72033987 None:intergenic 30.0%
TTGTAAGCAGTTAAAATAGC+AGG + chr8_1:72034071-72034090 None:intergenic 30.0%
!! ACAAAGCTACTGCTTTTAAA+AGG + chr8_1:72034137-72034156 None:intergenic 30.0%
ATATAATAGTGTAGTTGCGA+TGG - chr8_1:72034178-72034197 Msa1212170:intron 30.0%
! ACGGAAAATTTGCATTTTTC+AGG + chr8_1:72034319-72034338 None:intergenic 30.0%
!!! CTAGTTTTATGAGCATCTTT+TGG - chr8_1:72034351-72034370 Msa1212170:intron 30.0%
! GAGGAGAAAAAAACATTTTC+AGG + chr8_1:72034415-72034434 None:intergenic 30.0%
TGATTACATGAATCTCTTTC+TGG - chr8_1:72034797-72034816 Msa1212170:intron 30.0%
TCTTTAAATGACCTACATTG+GGG + chr8_1:72034846-72034865 None:intergenic 30.0%
!!! TCATTTGTTTTAGTTTGAGC+AGG - chr8_1:72034953-72034972 Msa1212170:intron 30.0%
! CATTTTCCCTATATGAGATT+TGG - chr8_1:72035212-72035231 Msa1212170:intron 30.0%
AATTTACAGGACATTGAAGT+TGG - chr8_1:72035280-72035299 Msa1212170:intron 30.0%
! TATTGGACTAGATTACAGAA+TGG + chr8_1:72035552-72035571 None:intergenic 30.0%
!! CTAGTCCAATAATGCTATTT+TGG - chr8_1:72035561-72035580 Msa1212170:five_prime_UTR 30.0%
! ATGAAGCTGGCTAGATTTTT+GGG + chr8_1:72032793-72032812 None:intergenic 35.0%
CAGCAGCTTATGATTGAAAA+TGG + chr8_1:72032829-72032848 None:intergenic 35.0%
ACAAAACGAAAACCCAAGAA+GGG + chr8_1:72032933-72032952 None:intergenic 35.0%
! TTTCTATTATGCGCCTTTTC+TGG - chr8_1:72032960-72032979 Msa1212170:CDS 35.0%
AACAGCCAAATCATCATAGA+TGG + chr8_1:72033033-72033052 None:intergenic 35.0%
!!! TTTTTGTTGGCTTCTTCTCA+TGG - chr8_1:72033580-72033599 Msa1212170:intron 35.0%
!! GTGTTTTTCAATTGTAGCTG+GGG - chr8_1:72033606-72033625 Msa1212170:intron 35.0%
TATTGGTGTATGGAACAAAC+TGG - chr8_1:72033757-72033776 Msa1212170:intron 35.0%
TCTATGGTAAGTGATCGTTA+TGG - chr8_1:72033796-72033815 Msa1212170:intron 35.0%
TCATAAAACTAGAGAAGAGC+GGG + chr8_1:72034343-72034362 None:intergenic 35.0%
CTCATAAAACTAGAGAAGAG+CGG + chr8_1:72034344-72034363 None:intergenic 35.0%
AGGTGCAATGAAAGAAAATG+AGG + chr8_1:72034395-72034414 None:intergenic 35.0%
!!! TTGTTTTAGTTTGAGCAGGT+TGG - chr8_1:72034957-72034976 Msa1212170:intron 35.0%
TTTCTGTGCAATATGTTTGC+AGG - chr8_1:72035112-72035131 Msa1212170:intron 35.0%
! TGCAGGTGATTATTCTTTTC+AGG - chr8_1:72035129-72035148 Msa1212170:intron 35.0%
CGCATATCAAGTGAAAAGTA+TGG + chr8_1:72035336-72035355 None:intergenic 35.0%
! TACTTTTCACTTGATATGCG+TGG - chr8_1:72035336-72035355 Msa1212170:intron 35.0%
TACACATTCTGTCTGCAAAT+AGG + chr8_1:72035391-72035410 None:intergenic 35.0%
AGCCGTCAACAATCAATCAA+TGG + chr8_1:72032758-72032777 None:intergenic 40.0%
! TGAAGCTGGCTAGATTTTTG+GGG + chr8_1:72032792-72032811 None:intergenic 40.0%
! GATGAAGCTGGCTAGATTTT+TGG + chr8_1:72032794-72032813 None:intergenic 40.0%
AAGGATAAGGAAGATGAAGC+TGG + chr8_1:72032806-72032825 None:intergenic 40.0%
TGGAAACGTTTATGAGCCAT+AGG + chr8_1:72032860-72032879 None:intergenic 40.0%
! CTCTGATTGGCTATCATTCA+TGG - chr8_1:72032884-72032903 Msa1212170:three_prime_UTR 40.0%
TGGCTATCATTCATGGAAAC+TGG - chr8_1:72032891-72032910 Msa1212170:three_prime_UTR 40.0%
CTATCATTCATGGAAACTGG+TGG - chr8_1:72032894-72032913 Msa1212170:three_prime_UTR 40.0%
ACGAAACGAAATTCCCTTCT+TGG - chr8_1:72032917-72032936 Msa1212170:three_prime_UTR 40.0%
CGAAACGAAATTCCCTTCTT+GGG - chr8_1:72032918-72032937 Msa1212170:three_prime_UTR 40.0%
GACAAAACGAAAACCCAAGA+AGG + chr8_1:72032934-72032953 None:intergenic 40.0%
!!! AATTCTTTTAGGGCCCTGTT+TGG - chr8_1:72033254-72033273 Msa1212170:CDS 40.0%
GAAACAGCTAATGAACATGC+CGG - chr8_1:72033439-72033458 Msa1212170:intron 40.0%
TCAAAGCAACACTTAGGTCA+GGG + chr8_1:72033554-72033573 None:intergenic 40.0%
!!! GCTTTGAGGTGTGTTTTTGT+TGG - chr8_1:72033567-72033586 Msa1212170:intron 40.0%
! CTACCAAAAGTGGTTTTCTC+CGG - chr8_1:72033668-72033687 Msa1212170:intron 40.0%
! TACCAAAAGTGGTTTTCTCC+GGG - chr8_1:72033669-72033688 Msa1212170:intron 40.0%
TTCCATACACCAATAAGCAC+GGG + chr8_1:72033752-72033771 None:intergenic 40.0%
GTTCCATACACCAATAAGCA+CGG + chr8_1:72033753-72033772 None:intergenic 40.0%
GCAGCACAAATCACAATACA+AGG + chr8_1:72033903-72033922 None:intergenic 40.0%
AATCCAGGCAGATTTCATAG+AGG - chr8_1:72034007-72034026 Msa1212170:intron 40.0%
! TTCATAGAGGAACTTGTTCG+TGG - chr8_1:72034020-72034039 Msa1212170:intron 40.0%
TGACAGTATCAAGAGTCGTT+TGG - chr8_1:72034213-72034232 Msa1212170:intron 40.0%
GTATCAAGAGTCGTTTGGTA+CGG - chr8_1:72034218-72034237 Msa1212170:intron 40.0%
!!! GTCTGAAGCTTGTTTTGCAA+AGG - chr8_1:72034650-72034669 Msa1212170:CDS 40.0%
GTTGCAAAAATCAACAAGGG+GGG + chr8_1:72034824-72034843 None:intergenic 40.0%
GGTTGCAAAAATCAACAAGG+GGG + chr8_1:72034825-72034844 None:intergenic 40.0%
GGGTTGCAAAAATCAACAAG+GGG + chr8_1:72034826-72034845 None:intergenic 40.0%
GGGGTTGCAAAAATCAACAA+GGG + chr8_1:72034827-72034846 None:intergenic 40.0%
TGGGGTTGCAAAAATCAACA+AGG + chr8_1:72034828-72034847 None:intergenic 40.0%
! GATTTTTGCAACCCCAATGT+AGG - chr8_1:72034832-72034851 Msa1212170:intron 40.0%
TGCTACAAACAAATCAACGC+AGG + chr8_1:72034909-72034928 None:intergenic 40.0%
ACTTGATATGCGTGGACAAA+TGG - chr8_1:72035344-72035363 Msa1212170:intron 40.0%
! TTATTTCTCCATGAAGTGGG+AGG - chr8_1:72035493-72035512 Msa1212170:five_prime_UTR 40.0%
TATTTCTCCATGAAGTGGGA+GGG - chr8_1:72035494-72035513 Msa1212170:five_prime_UTR 40.0%
ACGTTTCCACACTCTCTGAT+TGG - chr8_1:72032871-72032890 Msa1212170:three_prime_UTR 45.0%
TGATAGCCAATCAGAGAGTG+TGG + chr8_1:72032880-72032899 None:intergenic 45.0%
TGCAGTGAGAGTTCCAGAAA+AGG + chr8_1:72032976-72032995 None:intergenic 45.0%
! CTCACTGCATGTTTTGCCTT+TGG - chr8_1:72032987-72033006 Msa1212170:CDS 45.0%
CTCAAAGCAACACTTAGGTC+AGG + chr8_1:72033555-72033574 None:intergenic 45.0%
!! CTGACCTAAGTGTTGCTTTG+AGG - chr8_1:72033553-72033572 Msa1212170:intron 45.0%
CACACCTCAAAGCAACACTT+AGG + chr8_1:72033560-72033579 None:intergenic 45.0%
ACCAGCTATAGCTCCTGTAA+GGG + chr8_1:72033632-72033651 None:intergenic 45.0%
! ACCAAAAGTGGTTTTCTCCG+GGG - chr8_1:72033670-72033689 Msa1212170:intron 45.0%
! TTTTCTCCGGGGAGTTTCAT+TGG - chr8_1:72033681-72033700 Msa1212170:intron 45.0%
TTTCTCCGGGGAGTTTCATT+GGG - chr8_1:72033682-72033701 Msa1212170:intron 45.0%
TTCCCGTGCTTATTGGTGTA+TGG - chr8_1:72033747-72033766 Msa1212170:intron 45.0%
! ATGGAACAAACTGGTTCACG+CGG - chr8_1:72033766-72033785 Msa1212170:intron 45.0%
! GTATTGTGATTTGTGCTGCC+TGG - chr8_1:72033904-72033923 Msa1212170:intron 45.0%
GTTCCTCTATGAAATCTGCC+TGG + chr8_1:72034013-72034032 None:intergenic 45.0%
! TAGAGGAACTTGTTCGTGGA+AGG - chr8_1:72034024-72034043 Msa1212170:intron 45.0%
TTGGGGGAGAGATATAGAGA+AGG + chr8_1:72034108-72034127 None:intergenic 45.0%
! TGGTACGGCTAATTTGTGAC+AGG - chr8_1:72034233-72034252 Msa1212170:intron 45.0%
AAACTAGAGAAGAGCGGGAA+CGG + chr8_1:72034338-72034357 None:intergenic 45.0%
CACAAGCCAATCAAACTGGA+GGG + chr8_1:72034437-72034456 None:intergenic 45.0%
TCACCACAAGCCAATCAAAC+TGG + chr8_1:72034441-72034460 None:intergenic 45.0%
GTTGGAGTTGCTAATAACGC+TGG - chr8_1:72034975-72034994 Msa1212170:intron 45.0%
CATAGCTTAGTATCACCCAG+AGG - chr8_1:72035020-72035039 Msa1212170:intron 45.0%
TATCACCCAGAGGATTGTCA+CGG - chr8_1:72035030-72035049 Msa1212170:intron 45.0%
GAATAAGCTACCGCACCATA+TGG - chr8_1:72035061-72035080 Msa1212170:intron 45.0%
TCATGCCCTTCGAAACCATA+TGG + chr8_1:72035079-72035098 None:intergenic 45.0%
! TATGGTTTCGAAGGGCATGA+AGG - chr8_1:72035079-72035098 Msa1212170:intron 45.0%
! TGAAAAGTATGGTCGCAACG+AGG + chr8_1:72035325-72035344 None:intergenic 45.0%
ATTTCTCCATGAAGTGGGAG+GGG - chr8_1:72035495-72035514 Msa1212170:five_prime_UTR 45.0%
CACCAGCTATAGCTCCTGTA+AGG + chr8_1:72033633-72033652 None:intergenic 50.0%
! AGCTGGTGCTTTTGCAGCTA+AGG - chr8_1:72033645-72033664 Msa1212170:intron 50.0%
GCAGCTAAGGCTACCAAAAG+TGG - chr8_1:72033658-72033677 Msa1212170:intron 50.0%
! TCCCCGGAGAAAACCACTTT+TGG + chr8_1:72033674-72033693 None:intergenic 50.0%
!! GTTTCATTGGGTGCCATTGC+CGG - chr8_1:72033694-72033713 Msa1212170:intron 50.0%
!! TTTCATTGGGTGCCATTGCC+GGG - chr8_1:72033695-72033714 Msa1212170:intron 50.0%
! TATACTCTCGGTGGAGGTGT+TGG - chr8_1:72033720-72033739 Msa1212170:intron 50.0%
TGGAAGCTTCCCGTGCTTAT+TGG - chr8_1:72033740-72033759 Msa1212170:intron 50.0%
TCTCCTCCCTCCAGTTTGAT+TGG - chr8_1:72034428-72034447 Msa1212170:intron 50.0%
AAGCCAATCAAACTGGAGGG+AGG + chr8_1:72034434-72034453 None:intergenic 50.0%
CCACAAGCCAATCAAACTGG+AGG + chr8_1:72034438-72034457 None:intergenic 50.0%
!! CCTCCAGTTTGATTGGCTTG+TGG - chr8_1:72034435-72034454 Msa1212170:intron 50.0%
ATCACCCAGAGGATTGTCAC+GGG - chr8_1:72035031-72035050 Msa1212170:intron 50.0%
GAATCCCGTGACAATCCTCT+GGG + chr8_1:72035038-72035057 None:intergenic 50.0%
TGAATCCCGTGACAATCCTC+TGG + chr8_1:72035039-72035058 None:intergenic 50.0%
ACCGCACCATATGGTTTCGA+AGG - chr8_1:72035070-72035089 Msa1212170:intron 50.0%
CCCTTCGAAACCATATGGTG+CGG + chr8_1:72035074-72035093 None:intergenic 50.0%
CCGCACCATATGGTTTCGAA+GGG - chr8_1:72035071-72035090 Msa1212170:intron 50.0%
TTTCTCCATGAAGTGGGAGG+GGG - chr8_1:72035496-72035515 Msa1212170:five_prime_UTR 50.0%
AAATTCCCCCTCCCACTTCA+TGG + chr8_1:72035504-72035523 None:intergenic 50.0%
AGCTGCTGTTGCTGCACCTA+TGG - chr8_1:72032841-72032860 Msa1212170:three_prime_UTR 55.0%
ATTGTAGCTGGGGCCCTTAC+AGG - chr8_1:72033616-72033635 Msa1212170:intron 55.0%
GCCCTTACAGGAGCTATAGC+TGG - chr8_1:72033628-72033647 Msa1212170:intron 55.0%
TGGCACCCAATGAAACTCCC+CGG + chr8_1:72033690-72033709 None:intergenic 55.0%
!! TTCATTGGGTGCCATTGCCG+GGG - chr8_1:72033696-72033715 Msa1212170:intron 55.0%
CATTGCCGGGGCTATACTCT+CGG - chr8_1:72033708-72033727 Msa1212170:intron 55.0%
! TTCGAAGGGCATGAAGGCAG+AGG - chr8_1:72035085-72035104 Msa1212170:intron 55.0%
! CGAGAGTATAGCCCCGGCAA+TGG + chr8_1:72033710-72033729 None:intergenic 60.0%
! CTCCACCGAGAGTATAGCCC+CGG + chr8_1:72033716-72033735 None:intergenic 60.0%
TTCACGCGGCGCGTCTTCTA+TGG - chr8_1:72033780-72033799 Msa1212170:intron 60.0%
TGCCGGGGCTATACTCTCGG+TGG - chr8_1:72033711-72033730 Msa1212170:intron 65.0%
CGGGGCTATACTCTCGGTGG+AGG - chr8_1:72033714-72033733 Msa1212170:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 72032688 72035673 72032688 ID=Msa1212170;Name=Msa1212170
chr8_1 mRNA 72032688 72035673 72032688 ID=Msa1212170-mRNA-1;Parent=Msa1212170;Name=Msa1212170-mRNA-1;_AED=0.04;_eAED=0.04;_QI=215|1|1|1|1|1|5|262|219
chr8_1 exon 72032688 72033072 72032688 ID=Msa1212170-mRNA-1:exon:24289;Parent=Msa1212170-mRNA-1
chr8_1 exon 72033228 72033395 72033228 ID=Msa1212170-mRNA-1:exon:24288;Parent=Msa1212170-mRNA-1
chr8_1 exon 72034264 72034347 72034264 ID=Msa1212170-mRNA-1:exon:24287;Parent=Msa1212170-mRNA-1
chr8_1 exon 72034560 72034738 72034560 ID=Msa1212170-mRNA-1:exon:24286;Parent=Msa1212170-mRNA-1
chr8_1 exon 72035353 72035673 72035353 ID=Msa1212170-mRNA-1:exon:24285;Parent=Msa1212170-mRNA-1
chr8_1 five_prime_UTR 72035459 72035673 72035459 ID=Msa1212170-mRNA-1:five_prime_utr;Parent=Msa1212170-mRNA-1
chr8_1 CDS 72035353 72035458 72035353 ID=Msa1212170-mRNA-1:cds;Parent=Msa1212170-mRNA-1
chr8_1 CDS 72034560 72034738 72034560 ID=Msa1212170-mRNA-1:cds;Parent=Msa1212170-mRNA-1
chr8_1 CDS 72034264 72034347 72034264 ID=Msa1212170-mRNA-1:cds;Parent=Msa1212170-mRNA-1
chr8_1 CDS 72033228 72033395 72033228 ID=Msa1212170-mRNA-1:cds;Parent=Msa1212170-mRNA-1
chr8_1 CDS 72032950 72033072 72032950 ID=Msa1212170-mRNA-1:cds;Parent=Msa1212170-mRNA-1
chr8_1 three_prime_UTR 72032688 72032949 72032688 ID=Msa1212170-mRNA-1:three_prime_utr;Parent=Msa1212170-mRNA-1
Gene Sequence

>Msa1212170

ATGGAAACTGGTGGACGAAACGAAATTCCCTTCTTGGGTTTTCGTTTTGTCTCCAAATTTCTATTATGCGCCTTTTCTGGAACTCTCACTGCATGTTTTGCCTTTGCTGGGGCCCTTACAGGAGCTATAGCTGGTGCTTTTGCAGCTAAGGCTACCAAAAGTGGTTTTCTCCGGGGAGTTTCATTGGGTGCCATTGCCGGGGCTATACTCTCGGTGGAGGTGTTGGAAGCTTCCCGTGCTTATTGGTGTATGGAACAAACTGGTTCACGCGGCGCGTCTTCTATGGCAGATTTCATAGAGGAACTTGTTCGTGGAAGGCTTGTAGAAGAATCACTTACACCTGCTATTTTAACTGCTTACAATTTGCAATTTGAGCAGGTTGGAGTTGCTAATAACGCTGGCTATGATGAGATTCATGATGTTCATAGCTTAGTATCACCCAGAGGATTGTCACGGGATTCATTGAATAAGCTACCGCACCATATGGTTTCGAAGGGCATGAAGGCAGAGGATACTTTCTGTGCAATATGTTTGCAGGACATTGAAGTTGGAGAAGTTGCAAGAAGCTTACCTCGTTGCGACCATACTTTTCACTTGATATGCGTGGACAAATGGCTTGTCAAGAATGATTCGTGTCCTATTTGCAGACAGAATGTGTAG
Protein sequence

>Msa1212170

METGGRNEIPFLGFRFVSKFLLCAFSGTLTACFAFAGALTGAIAGAFAAKATKSGFLRGVSLGAIAGAILSVEVLEASRAYWCMEQTGSRGASSMADFIEELVRGRLVEESLTPAILTAYNLQFEQVGVANNAGYDEIHDVHSLVSPRGLSRDSLNKLPHHMVSKGMKAEDTFCAICLQDIEVGEVARSLPRCDHTFHLICVDKWLVKNDSCPICRQNV*