AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1118750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1118750 MtrunA17_Chr7g0253401 80.620 258 5 3 1 258 1 213 6.19e-146 407
Msa1118750 MtrunA17_Chr6g0461331 48.438 256 101 8 1 256 1 225 6.63e-62 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1118750 AT5G18130.1 32.069 290 155 8 1 258 1 280 1.29e-36 131
Msa1118750 AT3G03870.2 34.532 278 150 14 1 258 1 266 1.11e-32 120
Msa1118750 AT5G18130.2 28.125 256 143 7 1 225 1 246 2.67e-19 84.7
Msa1118750 AT3G03870.1 31.120 241 135 13 1 222 1 229 4.71e-16 75.5

Find 74 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTTTCAATTCTATAATT+TGG 0.043382 7_3:-67509899 None:intergenic
CAAATTTGTTGACATGAAAT+TGG 0.182807 7_3:+67508053 Msa1118750:CDS
AACTTTCAATTCTATAATTT+GGG 0.200867 7_3:-67509898 None:intergenic
CCGCTACCACTACTAGATTT+TGG 0.209211 7_3:-67508081 None:intergenic
GGCTACTATGATAGTGTTTA+AGG 0.244273 7_3:+67509628 Msa1118750:CDS
GTAAATTAAGTTACGGATTA+GGG 0.245606 7_3:+67509770 Msa1118750:three_prime_UTR
TTCTCCATGTTCTTCATTCT+AGG 0.255315 7_3:-67508147 None:intergenic
TGTAAATTAAGTTACGGATT+AGG 0.269378 7_3:+67509769 Msa1118750:three_prime_UTR
GTTGGAGCTATATGTTCTTT+TGG 0.285385 7_3:+67508258 Msa1118750:CDS
AATACAACAAAACACCTTAA+TGG 0.285946 7_3:+67507516 Msa1118750:five_prime_UTR
ACAATTTGTGTCTTGTTCTT+TGG 0.297991 7_3:+67508294 Msa1118750:CDS
AAAGAAAAGTTGGTTTATAT+AGG 0.320203 7_3:-67507668 None:intergenic
GGAACATGGAAAATTTGTTT+TGG 0.322740 7_3:-67507892 None:intergenic
GTTGATTTGTGAATCAAAAT+AGG 0.327634 7_3:-67507592 None:intergenic
GAACATGGAGAATGAAAATA+AGG 0.328452 7_3:+67508158 Msa1118750:CDS
TCTTTCACCAGCCCTTCATT+TGG 0.338022 7_3:-67507946 None:intergenic
AAGAAAAGTTGGTTTATATA+GGG 0.342407 7_3:-67507667 None:intergenic
ATGATGGGTTCAAATTGAAT+TGG 0.356134 7_3:-67507913 None:intergenic
TCGTGCCTTGATGAAACTTT+TGG 0.359313 7_3:+67509659 Msa1118750:three_prime_UTR
TAATACTACTTTGGTCTTGT+TGG 0.368197 7_3:-67508341 None:intergenic
AACAAGACCAAAGTAGTATT+AGG 0.368502 7_3:+67508343 Msa1118750:CDS
TTGGATGCTACAATTCAATT+AGG 0.371625 7_3:+67509678 Msa1118750:three_prime_UTR
ATTTGGAACCTTGTCAATGA+TGG 0.382204 7_3:-67507929 None:intergenic
AGCAGAGCTCACATAACTTA+TGG 0.392361 7_3:+67509604 Msa1118750:CDS
TGGGTGATCCTTTCTGATGA+TGG 0.393897 7_3:+67507745 Msa1118750:CDS
CAAGATCTAAGCTACCATTA+AGG 0.402135 7_3:-67507530 None:intergenic
TTGATCAGCTTCCTCTATCT+CGG 0.423812 7_3:-67507992 None:intergenic
GATTCAGATTCTTGTGAAGA+TGG 0.428584 7_3:+67507711 Msa1118750:exon
TCTTGAACCTAATACTACTT+TGG 0.449158 7_3:-67508350 None:intergenic
TGATCAGCTTCCTCTATCTC+GGG 0.452128 7_3:-67507991 None:intergenic
AATCCAAGTCCCCGAGATAG+AGG 0.452399 7_3:+67507981 Msa1118750:CDS
TTGTGGAAGTGGAGCTTGAC+AGG 0.453604 7_3:+67508234 Msa1118750:CDS
GAAGCATTTGCAGCAGTAAG+AGG 0.455785 7_3:+67509571 Msa1118750:CDS
ATTGACAAGGTTCCAAATGA+AGG 0.461067 7_3:+67507934 Msa1118750:CDS
GATTGTGATTGCAGAGGGTT+AGG 0.464099 7_3:+67509518 Msa1118750:intron
GTTATGTGAGCTCTGCTCAA+TGG 0.473473 7_3:-67509598 None:intergenic
AGAGTGATCAAAAGAAAAGT+TGG 0.473982 7_3:-67507678 None:intergenic
TTTGGAACCTTGTCAATGAT+GGG 0.478486 7_3:-67507928 None:intergenic
ATTCTATAATTTGGGGCAAG+TGG 0.499250 7_3:-67509890 None:intergenic
CAATGTAGATAAACAAATGA+GGG 0.501682 7_3:+67509737 Msa1118750:three_prime_UTR
CAAGGTTCCAAATGAAGGGC+TGG 0.510901 7_3:+67507939 Msa1118750:CDS
AAAAGTTGGTTTATATAGGG+AGG 0.517804 7_3:-67507664 None:intergenic
AAGGAAGGAGAGGACATGAA+TGG 0.517989 7_3:+67508201 Msa1118750:CDS
AAGTGGAGCTTGACAGGAGT+TGG 0.528199 7_3:+67508240 Msa1118750:CDS
ATCTCCATGTCTGCATCAAG+TGG 0.532042 7_3:-67508114 None:intergenic
TCAATGTAGATAAACAAATG+AGG 0.538382 7_3:+67509736 Msa1118750:three_prime_UTR
TTCTCCACTTGATGCAGACA+TGG 0.549790 7_3:+67508110 Msa1118750:CDS
AGCATCCAAAAGTTTCATCA+AGG 0.552106 7_3:-67509664 None:intergenic
CCAAAATCTAGTAGTGGTAG+CGG 0.558957 7_3:+67508081 Msa1118750:CDS
ATGTACTATGATGAGAAGGA+AGG 0.568648 7_3:+67508186 Msa1118750:CDS
TTAGGCACAATCTTCTATTG+TGG 0.569921 7_3:+67509696 Msa1118750:three_prime_UTR
ACTTTCAATTCTATAATTTG+GGG 0.579007 7_3:-67509897 None:intergenic
TTGACAAGGTTCCAAATGAA+GGG 0.592489 7_3:+67507935 Msa1118750:CDS
GTTCAAATTGAATTGGAACA+TGG 0.592625 7_3:-67507906 None:intergenic
AAACTCACTCGTCTTTGACA+AGG 0.596384 7_3:+67507810 Msa1118750:CDS
TGTTCTTTGGAAGTCAACAA+AGG 0.599769 7_3:+67508307 Msa1118750:CDS
TGTTATGTACTATGATGAGA+AGG 0.601438 7_3:+67508182 Msa1118750:CDS
ATTGCAGAGGGTTAGGCAAG+TGG 0.601634 7_3:+67509525 Msa1118750:intron
GAAGGAGAGGACATGAATGG+TGG 0.601946 7_3:+67508204 Msa1118750:CDS
CCTAACAACTACAATTACAA+TGG 0.611060 7_3:-67509949 None:intergenic
CAAGATCTACACTGATGACA+AGG 0.613940 7_3:+67508368 Msa1118750:CDS
TAAATTAAGTTACGGATTAG+GGG 0.619637 7_3:+67509771 Msa1118750:three_prime_UTR
ATTTGAACCCATCATTGACA+AGG 0.620426 7_3:+67507921 Msa1118750:CDS
AAAGAACAAGACACAAATTG+TGG 0.626637 7_3:-67508292 None:intergenic
GATCAGCTTCCTCTATCTCG+GGG 0.628424 7_3:-67507990 None:intergenic
CTATGATGAGAAGGAAGGAG+AGG 0.636243 7_3:+67508191 Msa1118750:CDS
ATCTCCTAGAATGAAGAACA+TGG 0.641567 7_3:+67508143 Msa1118750:CDS
GAGTCACCAAAATCTAGTAG+TGG 0.643722 7_3:+67508075 Msa1118750:CDS
GATTTGTGAATCAAAATAGG+TGG 0.659358 7_3:-67507589 None:intergenic
AGAGCTCACATAACTTATGG+TGG 0.659504 7_3:+67509607 Msa1118750:CDS
GTAGATAAACAAATGAGGGG+TGG 0.660529 7_3:+67509741 Msa1118750:three_prime_UTR
AATGTAGATAAACAAATGAG+GGG 0.663527 7_3:+67509738 Msa1118750:three_prime_UTR
GAAGATGGATATCAATGAGT+GGG 0.674672 7_3:+67507726 Msa1118750:exon
TGAAGATGGATATCAATGAG+TGG 0.698977 7_3:+67507725 Msa1118750:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAATGTATAAATAA+AGG + chr7_3:67508776-67508795 Msa1118750:intron 10.0%
!! AAGAAAAATGTATAAATAAA+GGG + chr7_3:67508777-67508796 Msa1118750:intron 10.0%
!!! ATATATGAGAATTTTAAAAT+AGG + chr7_3:67509029-67509048 Msa1118750:intron 10.0%
!! TCAATTATAAATAATTTATG+AGG + chr7_3:67509192-67509211 Msa1118750:intron 10.0%
!! CAATTATAAATAATTTATGA+GGG + chr7_3:67509193-67509212 Msa1118750:intron 10.0%
!! CTTGTTAATAATTTATAATT+AGG - chr7_3:67509268-67509287 None:intergenic 10.0%
!! TAAAAAATACTAGTAATAAA+AGG - chr7_3:67509798-67509817 None:intergenic 10.0%
!!! AATTTGCTTATAAAAAGAAA+CGG - chr7_3:67508615-67508634 None:intergenic 15.0%
!!! AATTTGCTTATAAAAAGAAA+TGG + chr7_3:67508615-67508634 Msa1118750:intron 15.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr7_3:67508778-67508797 Msa1118750:intron 15.0%
!! AGTATTAATTAGAGTTATAA+AGG + chr7_3:67508836-67508855 Msa1118750:intron 15.0%
!! GTATTAATTAGAGTTATAAA+GGG + chr7_3:67508837-67508856 Msa1118750:intron 15.0%
!! TATTAATTAGAGTTATAAAG+GGG + chr7_3:67508838-67508857 Msa1118750:intron 15.0%
!!! TTAAAAACTTTTTAGTTTAG+AGG + chr7_3:67509242-67509261 Msa1118750:intron 15.0%
!!! TTTAGAGATTTAAAAAGTAT+AGG + chr7_3:67509317-67509336 Msa1118750:intron 15.0%
!!! GTTTTATGTAAATTAAGTTA+CGG + chr7_3:67509763-67509782 Msa1118750:three_prime_UTR 15.0%
!!! ATTACTAGTATTTTTTAGTT+TGG + chr7_3:67509801-67509820 Msa1118750:three_prime_UTR 15.0%
!!! TTACTAGTATTTTTTAGTTT+GGG + chr7_3:67509802-67509821 Msa1118750:three_prime_UTR 15.0%
!!! TTTTTTGGTATAAATACTTT+GGG - chr7_3:67509862-67509881 None:intergenic 15.0%
!! AACTTTCAATTCTATAATTT+GGG - chr7_3:67509901-67509920 None:intergenic 15.0%
!!! AAGAAAAGTTGGTTTATATA+GGG - chr7_3:67507670-67507689 None:intergenic 20.0%
!!! AAAGAAAAGTTGGTTTATAT+AGG - chr7_3:67507671-67507690 None:intergenic 20.0%
!!! TTTTAATGGTTCAATATCAA+TGG - chr7_3:67507869-67507888 None:intergenic 20.0%
!! ACACTAGTATTAATTGAATA+TGG - chr7_3:67508698-67508717 None:intergenic 20.0%
!! TTACAAATATTTAAATGTCC+AGG - chr7_3:67509150-67509169 None:intergenic 20.0%
!! TTATTCTTGAATAGTCAAAA+TGG + chr7_3:67509436-67509455 Msa1118750:intron 20.0%
!!! TTTTTGGTATAAATACTTTG+GGG - chr7_3:67509861-67509880 None:intergenic 20.0%
!!! CTTTTTTGGTATAAATACTT+TGG - chr7_3:67509863-67509882 None:intergenic 20.0%
!! ACTTTCAATTCTATAATTTG+GGG - chr7_3:67509900-67509919 None:intergenic 20.0%
!! GAACTTTCAATTCTATAATT+TGG - chr7_3:67509902-67509921 None:intergenic 20.0%
!! TCTAAAAAATGAATGAATGA+TGG + chr7_3:67509920-67509939 Msa1118750:three_prime_UTR 20.0%
! AATACAACAAAACACCTTAA+TGG + chr7_3:67507516-67507535 Msa1118750:five_prime_UTR 25.0%
!!! GTTGATTTGTGAATCAAAAT+AGG - chr7_3:67507595-67507614 None:intergenic 25.0%
!!! AATGGTTTTTGTTGATTTTG+GGG - chr7_3:67507851-67507870 None:intergenic 25.0%
!!! CAATGGTTTTTGTTGATTTT+GGG - chr7_3:67507852-67507871 None:intergenic 25.0%
!!! TCAATGGTTTTTGTTGATTT+TGG - chr7_3:67507853-67507872 None:intergenic 25.0%
!!! ATTTGTTTTGGCACTTTTAA+TGG - chr7_3:67507883-67507902 None:intergenic 25.0%
!!! CAAATTTGTTGACATGAAAT+TGG + chr7_3:67508053-67508072 Msa1118750:CDS 25.0%
! CATTCAATTAATGATGTATG+TGG + chr7_3:67508542-67508561 Msa1118750:intron 25.0%
! ATGTATGTGGTTCATAATAT+GGG - chr7_3:67508568-67508587 None:intergenic 25.0%
! CATTCAATTAATGATGTATG+TGG - chr7_3:67508542-67508561 None:intergenic 25.0%
! TTGCTTATAAAAAGAAATGG+AGG + chr7_3:67508618-67508637 Msa1118750:intron 25.0%
! TGCTTATAAAAAGAAATGGA+GGG + chr7_3:67508619-67508638 Msa1118750:intron 25.0%
! AAATGTATAAATAAAGGGGA+AGG + chr7_3:67508782-67508801 Msa1118750:intron 25.0%
!! TTGAAATGACTCAGTTATTT+TGG + chr7_3:67508909-67508928 Msa1118750:intron 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG + chr7_3:67508910-67508929 Msa1118750:intron 25.0%
!!! GGACACTTTTTTTTAGTAAA+TGG + chr7_3:67508931-67508950 Msa1118750:intron 25.0%
! AAAATATTGTTTGAGCATCT+TGG + chr7_3:67509003-67509022 Msa1118750:intron 25.0%
!! GAGATTTAAAAAGTATAGGA+TGG + chr7_3:67509321-67509340 Msa1118750:intron 25.0%
! ACAATAGTGTCATAGAAAAA+AGG + chr7_3:67509375-67509394 Msa1118750:intron 25.0%
! CAATAGTGTCATAGAAAAAA+GGG + chr7_3:67509376-67509395 Msa1118750:intron 25.0%
! AATAGTGTCATAGAAAAAAG+GGG + chr7_3:67509377-67509396 Msa1118750:intron 25.0%
! TCAATGTAGATAAACAAATG+AGG + chr7_3:67509736-67509755 Msa1118750:three_prime_UTR 25.0%
! CAATGTAGATAAACAAATGA+GGG + chr7_3:67509737-67509756 Msa1118750:three_prime_UTR 25.0%
! AATGTAGATAAACAAATGAG+GGG + chr7_3:67509738-67509757 Msa1118750:three_prime_UTR 25.0%
! TGTAAATTAAGTTACGGATT+AGG + chr7_3:67509769-67509788 Msa1118750:three_prime_UTR 25.0%
!! GTAAATTAAGTTACGGATTA+GGG + chr7_3:67509770-67509789 Msa1118750:three_prime_UTR 25.0%
!!! TAAATTAAGTTACGGATTAG+GGG + chr7_3:67509771-67509790 Msa1118750:three_prime_UTR 25.0%
!!! TTTTTAGTTTGGGAATTCAA+AGG + chr7_3:67509812-67509831 Msa1118750:three_prime_UTR 25.0%
!! TGTTTTGTTGTATTGTAACG+TGG - chr7_3:67507510-67507529 None:intergenic 30.0%
GATTTGTGAATCAAAATAGG+TGG - chr7_3:67507592-67507611 None:intergenic 30.0%
! AAAAGTTGGTTTATATAGGG+AGG - chr7_3:67507667-67507686 None:intergenic 30.0%
AGAGTGATCAAAAGAAAAGT+TGG - chr7_3:67507681-67507700 None:intergenic 30.0%
!! GGAACATGGAAAATTTGTTT+TGG - chr7_3:67507895-67507914 None:intergenic 30.0%
GTTCAAATTGAATTGGAACA+TGG - chr7_3:67507909-67507928 None:intergenic 30.0%
!! ATGATGGGTTCAAATTGAAT+TGG - chr7_3:67507916-67507935 None:intergenic 30.0%
GAACATGGAGAATGAAAATA+AGG + chr7_3:67508158-67508177 Msa1118750:CDS 30.0%
TGTTATGTACTATGATGAGA+AGG + chr7_3:67508182-67508201 Msa1118750:CDS 30.0%
AAAGAACAAGACACAAATTG+TGG - chr7_3:67508295-67508314 None:intergenic 30.0%
! ACAATTTGTGTCTTGTTCTT+TGG + chr7_3:67508294-67508313 Msa1118750:CDS 30.0%
!! TAATACTACTTTGGTCTTGT+TGG - chr7_3:67508344-67508363 None:intergenic 30.0%
AACAAGACCAAAGTAGTATT+AGG + chr7_3:67508343-67508362 Msa1118750:CDS 30.0%
TCTTGAACCTAATACTACTT+TGG - chr7_3:67508353-67508372 None:intergenic 30.0%
AAGAGTTGACATAAAGATCT+TGG - chr7_3:67508475-67508494 None:intergenic 30.0%
TGCTTATAAAAAGAAACGGA+GGG - chr7_3:67508504-67508523 None:intergenic 30.0%
TTGCTTATAAAAAGAAACGG+AGG - chr7_3:67508505-67508524 None:intergenic 30.0%
GATGTATGTGGTTCATAATA+TGG - chr7_3:67508569-67508588 None:intergenic 30.0%
TCTATGGATAAGACTTGAAT+CGG + chr7_3:67508653-67508672 Msa1118750:intron 30.0%
ATTCAAAGCTGTTTACTTCA+GGG - chr7_3:67509122-67509141 None:intergenic 30.0%
! TAAAAAGTATAGGATGGTGA+TGG + chr7_3:67509327-67509346 Msa1118750:intron 30.0%
ATAGTGTCATAGAAAAAAGG+GGG + chr7_3:67509378-67509397 Msa1118750:intron 30.0%
TTGAATAGTCAAAATGGATC+AGG + chr7_3:67509442-67509461 Msa1118750:intron 30.0%
TGCAATCACAATCACAATTA+AGG - chr7_3:67509511-67509530 None:intergenic 30.0%
TTGGATGCTACAATTCAATT+AGG + chr7_3:67509678-67509697 Msa1118750:three_prime_UTR 30.0%
CAAGATCTAAGCTACCATTA+AGG - chr7_3:67507533-67507552 None:intergenic 35.0%
! AAATAGGTGGCTTTTATGTG+TGG - chr7_3:67507579-67507598 None:intergenic 35.0%
GATTCAGATTCTTGTGAAGA+TGG + chr7_3:67507711-67507730 Msa1118750:exon 35.0%
TGAAGATGGATATCAATGAG+TGG + chr7_3:67507725-67507744 Msa1118750:exon 35.0%
GAAGATGGATATCAATGAGT+GGG + chr7_3:67507726-67507745 Msa1118750:exon 35.0%
CAAAAAAGCCATCATCAGAA+AGG - chr7_3:67507756-67507775 None:intergenic 35.0%
!!! TCTGATGATGGCTTTTTTGA+TGG + chr7_3:67507757-67507776 Msa1118750:CDS 35.0%
ATTTGAACCCATCATTGACA+AGG + chr7_3:67507921-67507940 Msa1118750:CDS 35.0%
! TTTGGAACCTTGTCAATGAT+GGG - chr7_3:67507931-67507950 None:intergenic 35.0%
! ATTTGGAACCTTGTCAATGA+TGG - chr7_3:67507932-67507951 None:intergenic 35.0%
ATTGACAAGGTTCCAAATGA+AGG + chr7_3:67507934-67507953 Msa1118750:CDS 35.0%
TTGACAAGGTTCCAAATGAA+GGG + chr7_3:67507935-67507954 Msa1118750:CDS 35.0%
ATCTCCTAGAATGAAGAACA+TGG + chr7_3:67508143-67508162 Msa1118750:CDS 35.0%
TTCTCCATGTTCTTCATTCT+AGG - chr7_3:67508150-67508169 None:intergenic 35.0%
ATGTACTATGATGAGAAGGA+AGG + chr7_3:67508186-67508205 Msa1118750:CDS 35.0%
!!! TGAATGGTGGTTTTAACTTG+TGG + chr7_3:67508217-67508236 Msa1118750:CDS 35.0%
! GTTGGAGCTATATGTTCTTT+TGG + chr7_3:67508258-67508277 Msa1118750:CDS 35.0%
! TGTTCTTTGGAAGTCAACAA+AGG + chr7_3:67508307-67508326 Msa1118750:CDS 35.0%
AACTAATCTTTGCGCAGAAT+CGG + chr7_3:67508444-67508463 Msa1118750:intron 35.0%
!! GAGGGAGTATGTTTTATCTA+TGG + chr7_3:67508637-67508656 Msa1118750:intron 35.0%
ATAAGTGTGAAATGAGCCAA+GGG - chr7_3:67508756-67508775 None:intergenic 35.0%
AATAAGTGTGAAATGAGCCA+AGG - chr7_3:67508757-67508776 None:intergenic 35.0%
TGTATAAATAAAGGGGAAGG+TGG + chr7_3:67508785-67508804 Msa1118750:intron 35.0%
GAGTTATAAAGGGGAAAAAG+TGG + chr7_3:67508847-67508866 Msa1118750:intron 35.0%
AGTAAATGGCTCAGTTAATC+CGG + chr7_3:67508945-67508964 Msa1118750:intron 35.0%
GATTCAAAGCTGTTTACTTC+AGG - chr7_3:67509123-67509142 None:intergenic 35.0%
! GAGAAATTCCTGTCATTTGA+TGG + chr7_3:67509352-67509371 Msa1118750:intron 35.0%
ACTATTGTCCATCAAATGAC+AGG - chr7_3:67509363-67509382 None:intergenic 35.0%
! AAAAAAGGGGGTGATTGTTT+TGG + chr7_3:67509390-67509409 Msa1118750:intron 35.0%
AATTGTGATTGTGATTGCAG+AGG + chr7_3:67509512-67509531 Msa1118750:intron 35.0%
ATTGTGATTGTGATTGCAGA+GGG + chr7_3:67509513-67509532 Msa1118750:intron 35.0%
! GGCTACTATGATAGTGTTTA+AGG + chr7_3:67509628-67509647 Msa1118750:CDS 35.0%
AGCATCCAAAAGTTTCATCA+AGG - chr7_3:67509667-67509686 None:intergenic 35.0%
TTAGGCACAATCTTCTATTG+TGG + chr7_3:67509696-67509715 Msa1118750:three_prime_UTR 35.0%
!!! CAAGTGGCAACTTTCTTTTT+TGG - chr7_3:67509877-67509896 None:intergenic 35.0%
! ATTCTATAATTTGGGGCAAG+TGG - chr7_3:67509893-67509912 None:intergenic 35.0%
AAACTCACTCGTCTTTGACA+AGG + chr7_3:67507810-67507829 Msa1118750:CDS 40.0%
!!! GGTTTTTGTTGATTTTGGGG+AGG - chr7_3:67507848-67507867 None:intergenic 40.0%
TTGATCAGCTTCCTCTATCT+CGG - chr7_3:67507995-67508014 None:intergenic 40.0%
GAGTCACCAAAATCTAGTAG+TGG + chr7_3:67508075-67508094 Msa1118750:CDS 40.0%
CCAAAATCTAGTAGTGGTAG+CGG + chr7_3:67508081-67508100 Msa1118750:CDS 40.0%
!!! GTGGTTTTAACTTGTGGAAG+TGG + chr7_3:67508223-67508242 Msa1118750:CDS 40.0%
CAAGATCTACACTGATGACA+AGG + chr7_3:67508368-67508387 Msa1118750:CDS 40.0%
CTCAATTAATTCAGGACGGA+GGG - chr7_3:67508733-67508752 None:intergenic 40.0%
AGGGCTCAATTAATTCAGGA+CGG - chr7_3:67508737-67508756 None:intergenic 40.0%
TCCTGAATTAATTGAGCCCT+TGG + chr7_3:67508737-67508756 Msa1118750:intron 40.0%
GTAAATGGCTCAGTTAATCC+GGG + chr7_3:67508946-67508965 Msa1118750:intron 40.0%
AGAAAAATACTACTCCCTCC+CGG - chr7_3:67508967-67508986 None:intergenic 40.0%
!! AACAGCTTTGAATCAGATCC+TGG + chr7_3:67509129-67509148 Msa1118750:intron 40.0%
AGCAGAGCTCACATAACTTA+TGG + chr7_3:67509604-67509623 Msa1118750:CDS 40.0%
AGAGCTCACATAACTTATGG+TGG + chr7_3:67509607-67509626 Msa1118750:CDS 40.0%
! TCGTGCCTTGATGAAACTTT+TGG + chr7_3:67509659-67509678 Msa1118750:three_prime_UTR 40.0%
GTAGATAAACAAATGAGGGG+TGG + chr7_3:67509741-67509760 Msa1118750:three_prime_UTR 40.0%
TGGGTGATCCTTTCTGATGA+TGG + chr7_3:67507745-67507764 Msa1118750:CDS 45.0%
TCTTTCACCAGCCCTTCATT+TGG - chr7_3:67507949-67507968 None:intergenic 45.0%
TGATCAGCTTCCTCTATCTC+GGG - chr7_3:67507994-67508013 None:intergenic 45.0%
! CCGCTACCACTACTAGATTT+TGG - chr7_3:67508084-67508103 None:intergenic 45.0%
TTCTCCACTTGATGCAGACA+TGG + chr7_3:67508110-67508129 Msa1118750:CDS 45.0%
ATCTCCATGTCTGCATCAAG+TGG - chr7_3:67508117-67508136 None:intergenic 45.0%
CTATGATGAGAAGGAAGGAG+AGG + chr7_3:67508191-67508210 Msa1118750:CDS 45.0%
AAGGAAGGAGAGGACATGAA+TGG + chr7_3:67508201-67508220 Msa1118750:CDS 45.0%
GCTCAATTAATTCAGGACGG+AGG - chr7_3:67508734-67508753 None:intergenic 45.0%
GCCAAGGGCTCAATTAATTC+AGG - chr7_3:67508741-67508760 None:intergenic 45.0%
GATTGTGATTGCAGAGGGTT+AGG + chr7_3:67509518-67509537 Msa1118750:intron 45.0%
! GAAGCATTTGCAGCAGTAAG+AGG + chr7_3:67509571-67509590 Msa1118750:CDS 45.0%
GTTATGTGAGCTCTGCTCAA+TGG - chr7_3:67509601-67509620 None:intergenic 45.0%
CAAGGTTCCAAATGAAGGGC+TGG + chr7_3:67507939-67507958 Msa1118750:CDS 50.0%
AATCCAAGTCCCCGAGATAG+AGG + chr7_3:67507981-67508000 Msa1118750:CDS 50.0%
GATCAGCTTCCTCTATCTCG+GGG - chr7_3:67507993-67508012 None:intergenic 50.0%
GAAGGAGAGGACATGAATGG+TGG + chr7_3:67508204-67508223 Msa1118750:CDS 50.0%
TTGTGGAAGTGGAGCTTGAC+AGG + chr7_3:67508234-67508253 Msa1118750:CDS 50.0%
AAGTGGAGCTTGACAGGAGT+TGG + chr7_3:67508240-67508259 Msa1118750:CDS 50.0%
AATGGCTCAGTTAATCCGGG+AGG + chr7_3:67508949-67508968 Msa1118750:intron 50.0%
ATGGCTCAGTTAATCCGGGA+GGG + chr7_3:67508950-67508969 Msa1118750:intron 50.0%
ATTGCAGAGGGTTAGGCAAG+TGG + chr7_3:67509525-67509544 Msa1118750:intron 50.0%
CTTCCTCTATCTCGGGGACT+TGG - chr7_3:67507987-67508006 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 67507479 67509960 67507479 ID=Msa1118750;Name=Msa1118750
chr7_3 mRNA 67507479 67509960 67507479 ID=Msa1118750-mRNA-1;Parent=Msa1118750;Name=Msa1118750-mRNA-1;_AED=0.00;_eAED=0.00;_QI=251|1|1|1|1|1|2|312|258
chr7_3 exon 67507479 67508389 67507479 ID=Msa1118750-mRNA-1:exon:16618;Parent=Msa1118750-mRNA-1
chr7_3 exon 67509532 67509960 67509532 ID=Msa1118750-mRNA-1:exon:16619;Parent=Msa1118750-mRNA-1
chr7_3 five_prime_UTR 67507479 67507729 67507479 ID=Msa1118750-mRNA-1:five_prime_utr;Parent=Msa1118750-mRNA-1
chr7_3 CDS 67507730 67508389 67507730 ID=Msa1118750-mRNA-1:cds;Parent=Msa1118750-mRNA-1
chr7_3 CDS 67509532 67509648 67509532 ID=Msa1118750-mRNA-1:cds;Parent=Msa1118750-mRNA-1
chr7_3 three_prime_UTR 67509649 67509960 67509649 ID=Msa1118750-mRNA-1:three_prime_utr;Parent=Msa1118750-mRNA-1
Gene Sequence

>Msa1118750

ATGGATATCAATGAGTGGGTGATCCTTTCTGATGATGGCTTTTTTGATGGTGATGAGAAGCAAATTTTCTTAGATAAAAGAAACTCACTCGTCTTTGACAAGGATTACTTTTGCACCTCCCCAAAATCAACAAAAACCATTGATATTGAACCATTAAAAGTGCCAAAACAAATTTTCCATGTTCCAATTCAATTTGAACCCATCATTGACAAGGTTCCAAATGAAGGGCTGGTGAAAGAAAACACTGAAAAAATCCAAGTCCCCGAGATAGAGGAAGCTGATCAAGAGAGTGTTTCACAAGTTTTCTTTCATATAAAAGAGAACAAATTTGTTGACATGAAATTGGAGTCACCAAAATCTAGTAGTGGTAGCGGATTATTTTCTCCACTTGATGCAGACATGGAGATGATGACATCTCCTAGAATGAAGAACATGGAGAATGAAAATAAGGTTGTTATGTACTATGATGAGAAGGAAGGAGAGGACATGAATGGTGGTTTTAACTTGTGGAAGTGGAGCTTGACAGGAGTTGGAGCTATATGTTCTTTTGGTGTTGCTGCTGCCACAATTTGTGTCTTGTTCTTTGGAAGTCAACAAAGGAACAACAAACTCCAACAAGACCAAAGTAGTATTAGGTTCAAGATCTACACTGATGACAAGAGGGTTAGGCAAGTGGTACAACATGCAACAAAATTGAATGAAGCATTTGCAGCAGTAAGAGGTGTTCCATTGAGCAGAGCTCACATAACTTATGGTGGCTACTATGATAGTGTTTAA
Protein sequence

>Msa1118750

MDINEWVILSDDGFFDGDEKQIFLDKRNSLVFDKDYFCTSPKSTKTIDIEPLKVPKQIFHVPIQFEPIIDKVPNEGLVKENTEKIQVPEIEEADQESVSQVFFHIKENKFVDMKLESPKSSSGSGLFSPLDADMEMMTSPRMKNMENENKVVMYYDEKEGEDMNGGFNLWKWSLTGVGAICSFGVAAATICVLFFGSQQRNNKLQQDQSSIRFKIYTDDKRVRQVVQHATKLNEAFAAVRGVPLSRAHITYGGYYDSV*