AlfalfaGEDB | Msa1349730

M. sativa cultivar zhongmu-4 / Msa1349730

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349730	XP_003599451.1	96.000	350	14	0	1	350	1	350	0.0	706

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349730	G7IX31	96.000	350	14	0	1	350	1	350	0.0	706

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0354480	Msa1349730	0.813696	2.245915e-51	-8.615850e-47
Msa0448150	Msa1349730	0.927729	7.829150e-92	-8.615850e-47
Msa0486460	Msa1349730	0.804170	2.456908e-49	-8.615850e-47
Msa0827870	Msa1349730	0.803870	2.836286e-49	-8.615850e-47
Msa1170890	Msa1349730	0.811201	7.881118e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349730	MtrunA17_Chr3g0090061	96.000	350	14	0	1	350	1	350	0.0	706
Msa1349730	MtrunA17_Chr6g0486001	30.723	166	109	4	174	335	131	294	1.78e-16	79.0
Msa1349730	MtrunA17_Chr6g0483441	25.121	207	132	6	129	329	87	276	1.24e-15	76.3
Msa1349730	MtrunA17_Chr4g0053001	25.161	155	114	2	177	329	158	312	1.31e-15	76.6
Msa1349730	MtrunA17_Chr2g0331671	25.080	311	189	10	29	335	27	297	2.35e-15	75.9
Msa1349730	MtrunA17_Chr8g0392551	29.032	155	98	4	182	329	129	278	1.56e-13	70.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349730	AT3G08490.1	39.385	325	130	8	29	350	11	271	8.95e-61	196
Msa1349730	AT3G24600.1	29.730	148	102	2	179	325	135	281	1.10e-13	71.2
Msa1349730	AT1G45688.1	23.984	246	148	7	129	350	111	341	1.77e-12	68.2
Msa1349730	AT5G42860.1	23.265	245	151	6	129	350	89	319	2.11e-12	67.8
Msa1349730	AT1G45688.2	30.252	119	71	3	129	247	111	217	5.02e-11	62.8

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Find 106 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGATGATGATCACCATC+TGG	0.567321	tig0010977:+45982	Msa1349730:CDS
AAAGCCTAAGTTCCATCACC+GGG	0.638761	tig0010977:+47834	Msa1349730:CDS
AACACCCAAAATCTCCTTTG+AGG	0.489450	tig0010977:+46216	Msa1349730:CDS
AACTTAGGCTTTACAAGTGC+TGG	0.443593	tig0010977:-47823	None:intergenic
AAGATTACTTGTATCAATAT+TGG	0.308847	tig0010977:-47778	None:intergenic
AAGCACATGAATCAGAGTTA+TGG	0.515419	tig0010977:-46111	None:intergenic
AAGTTGATTCGGCGTCGTTT+TGG	0.320691	tig0010977:-45805	None:intergenic
AATAAGCCCATGTATGGTGC+TGG	0.508737	tig0010977:+47715	Msa1349730:CDS
AATTGACGCACTCACCTCAA+AGG	0.615175	tig0010977:-46230	None:intergenic
AATTTCATATCCATCTTAGG+AGG	0.559576	tig0010977:-46457	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTATGATGCATACCATTA+TGG	-	tig0010977:45670-45689	None:intergenic	30.0%
ATTATGAAAAAAATGGAGGG+TGG	+	tig0010977:46071-46090	Msa1349730:CDS	30.0%
AGGATTCCAGAATTTAAACT+AGG	+	tig0010977:46328-46347	Msa1349730:CDS	30.0%
GGATTCCAGAATTTAAACTA+GGG	+	tig0010977:46329-46348	Msa1349730:CDS	30.0%
GATTCCAGAATTTAAACTAG+GGG	+	tig0010977:46330-46349	Msa1349730:CDS	30.0%
AATTTCATATCCATCTTAGG+AGG	-	tig0010977:46460-46479	None:intergenic	30.0%
TAGCATATGTCCTTGTTAAA+TGG	+	tig0010977:46551-46570	Msa1349730:intron	30.0%
TAGTCTATGTCCATTTAACA+AGG	-	tig0010977:46564-46583	None:intergenic	30.0%
TTGCCATTAGAAATCACAAA+AGG	+	tig0010977:46660-46679	Msa1349730:intron	30.0%
AAAAATTGTCAAAGAGGCTT+AGG	-	tig0010977:46863-46882	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0010977	gene	45580	48157	45580	ID=Msa1349730;Name=Msa1349730
tig0010977	mRNA	45580	48157	45580	ID=Msa1349730-mRNA-1;Parent=Msa1349730;Name=Msa1349730-mRNA-1;_AED=0.03;_eAED=0.03;_QI=79\|1\|1\|1\|1\|1\|3\|215\|350
tig0010977	exon	45580	46237	45580	ID=Msa1349730-mRNA-1:exon:7069;Parent=Msa1349730-mRNA-1
tig0010977	exon	46322	46507	46322	ID=Msa1349730-mRNA-1:exon:7070;Parent=Msa1349730-mRNA-1
tig0010977	exon	47655	48157	47655	ID=Msa1349730-mRNA-1:exon:7071;Parent=Msa1349730-mRNA-1
tig0010977	five_prime_UTR	45580	45658	45580	ID=Msa1349730-mRNA-1:five_prime_utr;Parent=Msa1349730-mRNA-1
tig0010977	CDS	45659	46237	45659	ID=Msa1349730-mRNA-1:cds;Parent=Msa1349730-mRNA-1
tig0010977	CDS	46322	46507	46322	ID=Msa1349730-mRNA-1:cds;Parent=Msa1349730-mRNA-1
tig0010977	CDS	47655	47942	47655	ID=Msa1349730-mRNA-1:cds;Parent=Msa1349730-mRNA-1
tig0010977	three_prime_UTR	47943	48157	47943	ID=Msa1349730-mRNA-1:three_prime_utr;Parent=Msa1349730-mRNA-1

Gene Sequence

>Msa1349730

ATGTCTTACCATAATGGTATGCATCATAACAACAACGTTGTTGAAGATCATGAAACTCTGACATACCAAACCTACCCGTGTGGATACTATGTCCAAAGCCCCTCCACAGTCTCCCATGCAAATAGCATAGACAAACATAGCAACATCCAAAACGACGCCGAATCAACTTTCCACTCCCCGAACCGATCTGAGACCCACCGGACAAATCTGGCCCATGAACAAGAACCCAACCGATTTTCCCTTTGCCGCTACTCATCCTCTCGAGGCTCCAACAACTCTTTTTTGCACCACAAGAAGGTCTCATATGACGGCACTGCCACTGAAAACGATGATGATCACCATCTGGTGATCGTCAACGACAGTGGCAGTGTGGTCAGTGATGAGGATGAGAAAGGGTTGTTTGATGAGTATTATTATGAAAAAAATGGAGGGTGGAAGAGGTACTTCTCTTACCATAACTCTGATTCATGTGCTTGGATTTGGCTGCAAGTTACTTGGAGAGTTATGGTGAGTTTTGGATTTGCATTGCTTGTTTTCTACATTGCTACAAAACCTCCAACACCCAAAATCTCCTTTGAGATTGCAAGGATTCCAGAATTTAAACTAGGGGAAGGAGTGGATAGAACTGGAGTGACCACAAAGATTCTGACATGCAATTGCTCCATGAATTTAATCGTAGAGAATAAATCAAGATTTTTTGGTCTTCATATTCGTCCTCCTAAGATGGATATGAAATTTTCCATCTTACCCTTTGCATTTTCAATTGGACCTGAGCTCTACGCAGAAAGTGGATTAACCATCTTTCCATTGCAATTAGAGGCAAAGAATAAGCCCATGTATGGTGCTGGAAGAAGCATGGAGGACATGCTAGATTCTGGAAGAGGATTGCCAATATTGATACAAGTAATCTTGAGTTCAAGCTTTGAAGTGGTGCCAGCACTTGTAAAGCCTAAGTTCCATCACCGGGTAGAATGTATAGTGGTTCTTAAGAAGACTTACAATAAGAAACATCATTCCCAAGCATTTAATAGTACTTGTAAAGTAACTTCTTGA

Protein sequence

>Msa1349730

MSYHNGMHHNNNVVEDHETLTYQTYPCGYYVQSPSTVSHANSIDKHSNIQNDAESTFHSPNRSETHRTNLAHEQEPNRFSLCRYSSSRGSNNSFLHHKKVSYDGTATENDDDHHLVIVNDSGSVVSDEDEKGLFDEYYYEKNGGWKRYFSYHNSDSCAWIWLQVTWRVMVSFGFALLVFYIATKPPTPKISFEIARIPEFKLGEGVDRTGVTTKILTCNCSMNLIVENKSRFFGLHIRPPKMDMKFSILPFAFSIGPELYAESGLTIFPLQLEAKNKPMYGAGRSMEDMLDSGRGLPILIQVILSSSFEVVPALVKPKFHHRVECIVVLKKTYNKKHHSQAFNSTCKVTS*