AlfalfaGEDB | Msa1170890

M. sativa cultivar zhongmu-4 / Msa1170890

KEGG: K05909
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
PFAM: PF07732.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1170890	sp\|Q9SIY8\|LAC5_ARATH	76.316	570	126	3	7	567	11	580	0.0	932

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1170890	G7K780	98.765	567	7	0	1	567	1	567	0.0	1167

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0389460	Msa1170890	0.984048	1.935679e-159	-8.615850e-47
Msa0603120	Msa1170890	0.807964	3.907735e-50	-8.615850e-47
Msa0612350	Msa1170890	0.825269	5.139479e-54	-8.615850e-47
Msa0625780	Msa1170890	0.809096	2.240025e-50	-8.615850e-47
Msa0644150	Msa1170890	0.810229	1.278558e-50	-8.615850e-47
Msa0683960	Msa1170890	0.803154	3.991045e-49	-8.615850e-47
Msa0737690	Msa1170890	0.824200	9.183765e-54	-8.615850e-47
Msa0783850	Msa1170890	0.929704	4.740769e-93	-8.615850e-47
Msa0816830	Msa1170890	0.809418	1.911239e-50	-8.615850e-47
Msa0818720	Msa1170890	0.983115	7.179856e-157	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1170890	MtrunA17_Chr5g0437681	98.765	567	7	0	1	567	1	567	1167
Msa1170890	MtrunA17_Chr3g0114211	81.162	568	96	3	4	567	10	570	969
Msa1170890	MtrunA17_Chr5g0431581	59.507	568	221	4	1	567	1	560	728
Msa1170890	MtrunA17_Chr5g0404361	50.265	565	261	5	7	567	63	611	604
Msa1170890	MtrunA17_Chr5g0404341	50.970	567	256	7	7	567	16	566	595
Msa1170890	MtrunA17_Chr7g0238941	52.021	569	254	9	9	567	19	578	590
Msa1170890	MtrunA17_Chr7g0237491	51.215	576	263	11	4	567	11	580	585
Msa1170890	MtrunA17_Chr7g0238981	49.914	583	264	9	7	567	17	593	583
Msa1170890	MtrunA17_Chr4g0031901	52.057	559	245	11	7	551	20	569	580
Msa1170890	MtrunA17_Chr5g0429511	49.291	564	266	7	8	567	11	558	579

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1170890	AT2G40370.1	76.316	570	126	3	7	567	11	580	932
Msa1170890	AT5G05390.1	76.937	568	124	2	1	567	4	565	928
Msa1170890	AT2G30210.1	64.336	572	197	4	1	567	1	570	798
Msa1170890	AT5G07130.1	65.674	571	190	6	1	567	1	569	775
Msa1170890	AT5G07130.2	65.844	486	160	6	86	567	1	484	653
Msa1170890	AT5G07130.3	65.844	486	160	6	86	567	1	484	653
Msa1170890	AT5G09360.1	51.579	570	261	7	1	567	12	569	607
Msa1170890	AT2G29130.1	51.295	579	249	11	8	567	9	573	590
Msa1170890	AT5G60020.1	49.654	578	269	11	7	567	5	577	571
Msa1170890	AT5G03260.1	49.472	568	264	7	7	567	6	557	570

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Find 152 sgRNAs with CRISPR-Local

Find 518 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATATACGCTCATATT+TGG	0.224570	8_1:+265274	None:intergenic
AAACTCGTGCTCATGAACTT+TGG	0.490870	8_1:+269227	None:intergenic
AAAGTTACTAAGTTGAGATT+TGG	0.284792	8_1:-266448	Msa1170890:CDS
AACCATAAACTAACAGTAGT+AGG	0.497147	8_1:-267069	Msa1170890:CDS
AATCAATGTCAAGGACCGAA+CGG	0.668939	8_1:-266655	Msa1170890:CDS
AATCACCCTATTCATCTTCA+TGG	0.351556	8_1:-266376	Msa1170890:CDS
AATGTTCTCATGTTAGGACC+TGG	0.640008	8_1:-267012	Msa1170890:CDS
AATTAATAATGGAGATACTT+TGG	0.377624	8_1:-269016	Msa1170890:CDS
ACAACCAATGTTCTCATGTT+AGG	0.341290	8_1:-267018	Msa1170890:CDS
ACAATTGTTCCAATTGATTC+AGG	0.259031	8_1:-267156	Msa1170890:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAAACAATGTGTGAATGA+AGG	+	chr8_1:265297-265316	None:intergenic	30.0%
TAAATGAGTTAAGACAAGTC+AGG	-	chr8_1:266058-266077	Msa1170890:intron	30.0%
AAATGAGTTAAGACAAGTCA+GGG	-	chr8_1:266059-266078	Msa1170890:intron	30.0%
TGATCATTACCTAGAAGAAT+GGG	+	chr8_1:266622-266641	None:intergenic	30.0%
ATGATCATTACCTAGAAGAA+TGG	+	chr8_1:266623-266642	None:intergenic	30.0%
GACAAAGATTATGTTCATGA+TGG	-	chr8_1:266892-266911	Msa1170890:CDS	30.0%
GTGTATGCATCAGATATATT+TGG	+	chr8_1:267027-267046	None:intergenic	30.0%
TCTGATGCATACACTATTAA+TGG	-	chr8_1:267033-267052	Msa1170890:CDS	30.0%
TTTGCAATTGTGAAGAAAAG+AGG	+	chr8_1:267542-267561	None:intergenic	30.0%
AACCATAAACTAACAGTAGT+AGG	-	chr8_1:267560-267579	Msa1170890:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	265274	269377	265274	ID=Msa1170890;Name=Msa1170890
chr8_1	mRNA	265274	269377	265274	ID=Msa1170890-mRNA-1;Parent=Msa1170890;Name=Msa1170890-mRNA-1;_AED=0.05;_eAED=0.05;_QI=67\|1\|1\|1\|1\|1\|6\|306\|567
chr8_1	exon	265274	265710	265274	ID=Msa1170890-mRNA-1:exon:56;Parent=Msa1170890-mRNA-1
chr8_1	exon	266224	267183	266224	ID=Msa1170890-mRNA-1:exon:55;Parent=Msa1170890-mRNA-1
chr8_1	exon	267561	267689	267561	ID=Msa1170890-mRNA-1:exon:54;Parent=Msa1170890-mRNA-1
chr8_1	exon	268020	268264	268020	ID=Msa1170890-mRNA-1:exon:53;Parent=Msa1170890-mRNA-1
chr8_1	exon	268970	269121	268970	ID=Msa1170890-mRNA-1:exon:52;Parent=Msa1170890-mRNA-1
chr8_1	exon	269224	269377	269224	ID=Msa1170890-mRNA-1:exon:51;Parent=Msa1170890-mRNA-1
chr8_1	five_prime_UTR	269311	269377	269311	ID=Msa1170890-mRNA-1:five_prime_utr;Parent=Msa1170890-mRNA-1
chr8_1	CDS	269224	269310	269224	ID=Msa1170890-mRNA-1:cds;Parent=Msa1170890-mRNA-1
chr8_1	CDS	268970	269121	268970	ID=Msa1170890-mRNA-1:cds;Parent=Msa1170890-mRNA-1
chr8_1	CDS	268020	268264	268020	ID=Msa1170890-mRNA-1:cds;Parent=Msa1170890-mRNA-1
chr8_1	CDS	267561	267689	267561	ID=Msa1170890-mRNA-1:cds;Parent=Msa1170890-mRNA-1
chr8_1	CDS	266224	267183	266224	ID=Msa1170890-mRNA-1:cds;Parent=Msa1170890-mRNA-1
chr8_1	CDS	265580	265710	265580	ID=Msa1170890-mRNA-1:cds;Parent=Msa1170890-mRNA-1
chr8_1	three_prime_UTR	265274	265579	265274	ID=Msa1170890-mRNA-1:three_prime_utr;Parent=Msa1170890-mRNA-1

Gene Sequence

>Msa1170890

ATGGAAGCTTTCAACACCATCTTCTTAGCAATTTTTCTTCTCTTGGCTTCTTTAGCTAATGCCAAAGTTCATGAGCACGAGTTTGTCGTACAAGCAACTCCAGTGAAGAGGCTGTGCAACACACAAATGAGCATCACTGTGAATGGACAATTTCCTGGTCCAACTTTGGAAATTAATAATGGAGATACTTTGGTTGTCAAAGTCATTAACAAAGCTCGTTACAATGTCACCATTCATTGGCATGGGGTTAGACAAATCAGAACAGGATGGGCTGATGGACCAGAATTTGTGACTCAATGTCCTATTCGTCCTGGAGAAAGTTACACCTATAGATTTACCATTAATGGACAAGAAGGTACACTTTGGTGGCATGCTCATAGTTCATGGCTTAGAGCCACAGTTTATGGAGCTCTAATCATTCACCCTAAGGAAGGAGATGCTTATCCTTTCACTAAGCCAAAGCGTGAAACACCCATTCTTCTAGGGGAATGGTGGGATGCAAACCCTATGGATGTTGTGAGACAAGCCACACAAACAGGAGCAGCTCCAAATATATCTGATGCATACACTATTAATGGTCAACCTGGTGATCTTTACAAGTGCTCTACCAAAGGCACAACAATTGTTCCAATTGATTCAGGAGAAACAAACCTCCTAAGAGTAATCAATGCTGCACTAAATCAACCTCTTTTCTTCACAATTGCAAACCATAAACTAACAGTAGTAGGTGCTGATGCTTCCTATGTCAAACCATTCACAACCAATGTTCTCATGTTAGGACCTGGCCAAACCACTGATGTTCTAATCCAAGGTGACCAACCACCATCAAGATACTACATCGCCGGGCGCGCTTACCAATCGGCTCAAAATGCTGCGTTCGATAACACAACAACCACTGCCATTCTTGAATACAAATCATCACCTTGTCCTGCAAAGGGGGGTGCAAATATCAGACCAATCATGCCTTCATTACCTGCTTACAATGACACAAACACTGTTACATCATTTAGTAAAAGCTTTAGAAGTTTAAGGAATGTTGAAGTTCCTAATGAAATTGATGAAGATCTTTTCTTTACCATTGGATTAGGACTCAACAATTGTCCATCGAATTTTAATGCAAATCAATGTCAAGGACCGAACGGTACACGGTTCACTTCTAGTATGAACAATGTGTCTTTTGTACTTCCTAGCAATTTTTCAATCCTGCAGGCTCATAAACTTGGAGTACAAGGTGTTTTTACGACGGATTTTCCCACAAAACCTCCCGTGCAATTTGATTACACCGGTAATGTGAGTCGGTCATTGTGGCAACCGATTCAAGGGACTAAAGTTACTAAGTTGAGATTTGGATCAAGGGTTCAGGTTGTTTTGCAAGATACTAGTATTGTTACTCCTGAGAATCACCCTATTCATCTTCATGGATATGATTTCTATATTGTTGCTGAGGGATTTGGAAATTATGACCCTAAGAAAGATGCTTCAAAATTTAACCTTGTTGATCCACCTATGAGGAACACTGTTGCTGTACCTGTTAATGGTTGGGCAGTTATCAGATTTGTTGCTGACAATCCAGGTGTTTGGATTATGCATTGTCACTTGGATGTTCACATTGGATGGGGTTTGGCTACTGTGTTTTTGGTAGAGGATGGAGAAGGGCTATTGGAGTCCATAGAAGCTCCTCCTGAGGACCTGCCTTTGTGTTAG

Protein sequence

>Msa1170890

MEAFNTIFLAIFLLLASLANAKVHEHEFVVQATPVKRLCNTQMSITVNGQFPGPTLEINNGDTLVVKVINKARYNVTIHWHGVRQIRTGWADGPEFVTQCPIRPGESYTYRFTINGQEGTLWWHAHSSWLRATVYGALIIHPKEGDAYPFTKPKRETPILLGEWWDANPMDVVRQATQTGAAPNISDAYTINGQPGDLYKCSTKGTTIVPIDSGETNLLRVINAALNQPLFFTIANHKLTVVGADASYVKPFTTNVLMLGPGQTTDVLIQGDQPPSRYYIAGRAYQSAQNAAFDNTTTTAILEYKSSPCPAKGGANIRPIMPSLPAYNDTNTVTSFSKSFRSLRNVEVPNEIDEDLFFTIGLGLNNCPSNFNANQCQGPNGTRFTSSMNNVSFVLPSNFSILQAHKLGVQGVFTTDFPTKPPVQFDYTGNVSRSLWQPIQGTKVTKLRFGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNYDPKKDASKFNLVDPPMRNTVAVPVNGWAVIRFVADNPGVWIMHCHLDVHIGWGLATVFLVEDGEGLLESIEAPPEDLPLC*