AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1351480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351480 MtrunA17_Chr8g0357241 94.064 219 13 0 1 219 1 219 8.77e-153 422
Msa1351480 MtrunA17_Chr8g0357211 94.064 219 13 0 1 219 1 219 2.81e-152 421
Msa1351480 MtrunA17_Chr2g0312681 67.123 219 72 0 1 219 1 219 1.09e-107 308
Msa1351480 MtrunA17_Chr2g0312771 64.840 219 77 0 1 219 1 219 2.02e-104 300
Msa1351480 MtrunA17_Chr2g0312691 65.753 219 75 0 1 219 1 219 3.69e-104 300
Msa1351480 MtrunA17_Chr2g0312751 65.297 219 76 0 1 219 1 219 4.17e-104 299
Msa1351480 MtrunA17_Chr2g0312781 64.953 214 75 0 1 214 18 231 1.30e-101 293
Msa1351480 MtrunA17_Chr2g0312711 63.014 219 80 1 1 219 1 218 4.62e-97 281
Msa1351480 MtrunA17_Chr2g0312731 59.361 219 89 0 1 219 1 219 1.76e-96 280
Msa1351480 MtrunA17_Chr2g0312791 60.909 220 84 2 1 218 1 220 7.55e-94 273
Msa1351480 MtrunA17_Chr2g0312811 60.185 216 84 1 1 214 1 216 2.50e-92 270
Msa1351480 MtrunA17_Chr3g0107731 54.884 215 97 0 5 219 10 224 9.32e-92 268
Msa1351480 MtrunA17_Chr3g0107741 54.378 217 99 0 3 219 8 224 1.20e-90 265
Msa1351480 MtrunA17_Chr2g0312721 55.869 213 94 0 1 213 1 213 8.52e-84 248
Msa1351480 MtrunA17_Chr4g0069471 65.027 183 61 2 1 182 22 202 2.51e-82 243
Msa1351480 MtrunA17_Chr5g0433801 50.935 214 103 2 2 214 4 216 2.12e-71 216
Msa1351480 MtrunA17_Chr1g0196661 39.901 203 117 3 11 209 10 211 6.32e-44 147
Msa1351480 MtrunA17_Chr4g0029341 39.906 213 123 3 1 209 1 212 1.51e-43 145
Msa1351480 MtrunA17_Chr7g0240681 38.636 220 128 5 3 217 6 223 2.03e-43 145
Msa1351480 MtrunA17_Chr5g0415411 44.949 198 94 6 2 187 3 197 4.08e-43 144
Msa1351480 MtrunA17_Chr5g0417011 34.597 211 131 2 2 205 5 215 6.51e-43 144
Msa1351480 MtrunA17_Chr4g0008781 37.799 209 125 4 3 208 7 213 1.29e-42 143
Msa1351480 MtrunA17_Chr7g0240641 38.756 209 123 4 3 208 6 212 5.94e-42 141
Msa1351480 MtrunA17_Chr8g0379581 35.484 217 137 2 2 216 3 218 7.17e-42 141
Msa1351480 MtrunA17_Chr7g0240691 39.726 219 123 5 3 217 36 249 7.47e-42 142
Msa1351480 MtrunA17_Chr7g0240631 37.321 209 126 4 3 208 6 212 1.47e-41 140
Msa1351480 MtrunA17_Chr3g0132581 45.455 176 94 2 1 175 1 175 2.17e-41 140
Msa1351480 MtrunA17_Chr7g0240611 44.624 186 98 5 3 187 6 187 4.45e-41 139
Msa1351480 MtrunA17_Chr4g0008791 38.942 208 122 4 4 208 7 212 1.61e-40 138
Msa1351480 MtrunA17_Chr1g0196721 41.089 202 114 3 11 208 9 209 1.43e-39 135
Msa1351480 MtrunA17_Chr1g0196691 38.605 215 118 5 4 209 28 237 1.53e-38 133
Msa1351480 MtrunA17_Chr7g0240671 46.061 165 84 4 1 162 1 163 3.77e-38 132
Msa1351480 MtrunA17_Chr8g0379561 39.779 181 106 2 7 185 9 188 5.32e-38 131
Msa1351480 MtrunA17_Chr7g0240531 38.756 209 118 6 13 217 16 218 8.40e-38 131
Msa1351480 MtrunA17_Chr1g0196711 40.594 202 115 4 11 208 10 210 9.30e-38 130
Msa1351480 MtrunA17_Chr8g0363751 40.404 198 109 5 7 195 8 205 1.14e-37 130
Msa1351480 MtrunA17_Chr7g0240541 37.500 208 122 5 13 217 16 218 2.46e-37 129
Msa1351480 MtrunA17_Chr7g0240561 38.806 201 116 5 3 199 6 203 3.70e-37 129
Msa1351480 MtrunA17_Chr7g0240511 38.942 208 114 7 13 217 16 213 2.47e-36 127
Msa1351480 MtrunA17_Chr6g0483141 37.864 206 118 5 18 216 17 219 6.07e-36 126
Msa1351480 MtrunA17_Chr1g0196771 36.019 211 127 5 11 216 10 217 1.60e-35 125
Msa1351480 MtrunA17_Chr7g0240551 36.538 208 124 5 13 217 16 218 1.64e-35 125
Msa1351480 MtrunA17_Chr1g0212301 38.119 202 115 4 7 201 6 204 2.05e-35 124
Msa1351480 MtrunA17_Chr7g0240651 45.679 162 83 4 3 161 6 165 5.14e-35 124
Msa1351480 MtrunA17_Chr7g0240581 40.212 189 106 5 3 187 6 191 7.27e-34 120
Msa1351480 MtrunA17_Chr7g0240661 37.799 209 121 5 3 208 6 208 5.90e-33 118
Msa1351480 MtrunA17_Chr7g0240521 37.234 188 113 4 3 187 13 198 2.93e-31 114
Msa1351480 MtrunA17_Chr8g0391631 42.105 152 81 4 7 151 24 175 3.29e-31 112
Msa1351480 MtrunA17_Chr2g0313561 66.667 72 24 0 47 118 1 72 4.53e-30 106
Msa1351480 MtrunA17_Chr7g0263221 29.108 213 133 8 10 216 32 232 2.97e-13 66.6
Msa1351480 MtrunA17_Chr5g0415421 45.205 73 38 1 28 98 11 83 3.47e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351480 AT1G78380.1 63.889 216 78 0 1 216 1 216 2.57e-99 288
Msa1351480 AT1G78370.1 60.000 215 86 0 1 215 1 215 1.08e-96 281
Msa1351480 AT1G17170.1 60.731 219 85 1 1 219 1 218 7.15e-96 279
Msa1351480 AT1G17180.1 59.817 219 88 0 1 219 1 219 7.92e-96 279
Msa1351480 AT1G78340.1 61.215 214 83 0 1 214 1 214 1.27e-94 276
Msa1351480 AT1G78360.1 60.000 220 87 1 1 219 1 220 2.74e-94 275
Msa1351480 AT1G78320.1 59.813 214 86 0 1 214 1 214 3.07e-89 262
Msa1351480 AT1G17190.1 55.140 214 96 0 3 216 4 217 1.47e-85 253
Msa1351480 AT1G53680.1 52.995 217 100 2 5 219 8 224 1.86e-83 248
Msa1351480 AT3G43800.1 53.774 212 94 3 3 210 4 215 3.02e-74 224
Msa1351480 AT1G78320.2 50.935 214 78 1 1 214 1 187 1.49e-68 209
Msa1351480 AT3G09270.1 46.809 188 97 2 3 187 5 192 4.29e-47 155
Msa1351480 AT2G29420.1 39.048 210 125 2 2 209 7 215 8.57e-47 154
Msa1351480 AT1G10360.1 42.051 195 105 4 2 188 3 197 4.76e-43 145
Msa1351480 AT1G74590.1 34.545 220 132 4 5 215 8 224 5.71e-43 145
Msa1351480 AT1G10370.1 38.863 211 121 4 7 209 8 218 6.94e-43 144
Msa1351480 AT1G59670.1 40.090 222 124 5 3 215 5 226 2.32e-41 140
Msa1351480 AT2G29460.1 40.291 206 117 4 3 204 6 209 3.42e-41 140
Msa1351480 AT1G59700.1 39.462 223 124 6 3 215 5 226 1.11e-40 139
Msa1351480 AT2G29480.1 44.720 161 85 2 3 160 6 165 2.27e-40 138
Msa1351480 AT2G29490.1 45.342 161 84 2 3 160 6 165 2.28e-40 138
Msa1351480 AT2G29450.1 48.466 163 77 4 3 160 5 165 4.21e-40 137
Msa1351480 AT2G29440.1 47.273 165 75 4 3 160 5 164 5.73e-40 137
Msa1351480 AT1G27140.1 39.512 205 114 4 15 209 17 221 1.36e-39 136
Msa1351480 AT1G27130.1 35.747 221 130 5 7 218 9 226 5.04e-39 134
Msa1351480 AT2G29470.1 39.000 200 115 5 7 201 10 207 5.26e-39 134
Msa1351480 AT5G62480.1 36.872 179 105 1 5 175 9 187 5.53e-38 132
Msa1351480 AT1G69920.1 35.426 223 128 6 7 218 37 254 1.20e-36 129
Msa1351480 AT1G69930.1 34.762 210 130 3 7 209 15 224 1.14e-34 123
Msa1351480 AT5G62480.3 28.492 179 93 2 5 175 9 160 1.57e-20 86.3
Msa1351480 AT5G62480.2 28.492 179 94 2 5 175 9 161 2.06e-20 85.9

Find 65 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGCTCAAGCAAGATTT+TGG 0.160142 tig0012275:+104803 Msa1351480:CDS
ATGATGATGGATTCACAAAT+TGG 0.232208 tig0012275:-104718 None:intergenic
AGGATAGCATTGGCTGAAAA+AGG 0.234872 tig0012275:+104589 Msa1351480:CDS
GAAATGTAAACTAAACAATT+TGG 0.242177 tig0012275:+105671 Msa1351480:three_prime_UTR
CTTGGAGACCAACCTTATTT+TGG 0.247910 tig0012275:+105386 Msa1351480:CDS
TTGAAAAGAACAAGGCTTTC+AGG 0.250379 tig0012275:-104480 None:intergenic
GTGATGTTGGAGGGAGGATA+TGG 0.264890 tig0012275:+105291 Msa1351480:CDS
TTTCTCATCCGCAACAGATT+TGG 0.278908 tig0012275:-105558 None:intergenic
ACTGGAATCTTCTTGTGAAT+TGG 0.280000 tig0012275:-104676 None:intergenic
CTTTGATCCCTTTCTATAGT+TGG 0.290757 tig0012275:+105438 Msa1351480:CDS
CTATAGGAAGAGGTTTGATT+TGG 0.319582 tig0012275:+105604 Msa1351480:CDS
GATGAATGGCTTAGTATGTT+TGG 0.324709 tig0012275:+104556 Msa1351480:CDS
CATTATACATATAACATATA+TGG 0.336167 tig0012275:-105907 None:intergenic
AGAGTTGATTGATGGCTTAA+AGG 0.336739 tig0012275:+105349 Msa1351480:CDS
GATTCATTTGCAAAAGCATT+TGG 0.343663 tig0012275:-104654 None:intergenic
GGTTGCTCCTATTAGTGCTT+TGG 0.353192 tig0012275:+105692 Msa1351480:three_prime_UTR
CTTGCTTGAGCTTTCTCATA+AGG 0.355050 tig0012275:-104796 None:intergenic
GCAAAGAAAGAGTTGATTGA+TGG 0.384134 tig0012275:+105341 Msa1351480:CDS
TATTTGACCAAAGCACTAAT+AGG 0.392230 tig0012275:-105699 None:intergenic
TATGTATAATGTATTTATGT+AGG 0.399343 tig0012275:+105919 Msa1351480:three_prime_UTR
TTTCCCTTATGGATAAGAAC+TGG 0.413273 tig0012275:-104694 None:intergenic
AAAGAGAGATGAGATTGAAT+TGG 0.418834 tig0012275:+105319 Msa1351480:CDS
CTCCTCCTCGTAGTTAAATC+AGG 0.426001 tig0012275:-105884 None:intergenic
GATTCCAGTTCTTATCCATA+AGG 0.427078 tig0012275:+104690 Msa1351480:CDS
TTGAGTATATTGATGAGGTT+TGG 0.428745 tig0012275:+104743 Msa1351480:CDS
AGTTAATATTGTTAGATGAA+TGG 0.437571 tig0012275:+104542 Msa1351480:CDS
AATGTGTCACCACCAAAATA+AGG 0.439985 tig0012275:-105398 None:intergenic
TGATGAGGTTTGGAAGGATA+AGG 0.440755 tig0012275:+104753 Msa1351480:CDS
TTTAGGATCTGAGTACAGAT+TGG 0.455192 tig0012275:-105853 None:intergenic
TTCCTTCCTTTGTTGCTATG+TGG 0.458672 tig0012275:+105818 Msa1351480:three_prime_UTR
ATTCCAGTTCTTATCCATAA+GGG 0.462545 tig0012275:+104691 Msa1351480:CDS
TGATGTTGGAGGGAGGATAT+GGG 0.462736 tig0012275:+105292 Msa1351480:CDS
TTTGGACTGAGAAGACACTT+TGG 0.480983 tig0012275:+105622 Msa1351480:exon
AAAATGCAGGTTGGTGATGT+TGG 0.481244 tig0012275:+105278 Msa1351480:intron
TATAAAACCAACTATAGAAA+GGG 0.488530 tig0012275:-105445 None:intergenic
AATATACTCAACAATGATGA+TGG 0.489877 tig0012275:-104731 None:intergenic
GAGTACAGATTGGAGGTGAT+TGG 0.492012 tig0012275:-105843 None:intergenic
AAGGAATTAGAGAATGTGCT+TGG 0.497037 tig0012275:+105368 Msa1351480:CDS
GTATAAAACCAACTATAGAA+AGG 0.503827 tig0012275:-105446 None:intergenic
AATATTAACTCCTCTGCCAT+TGG 0.514154 tig0012275:-104529 None:intergenic
AGGGATTAAGTATGAGTACA+AGG 0.524364 tig0012275:+104609 Msa1351480:CDS
AGGATCTGAGTACAGATTGG+AGG 0.533882 tig0012275:-105850 None:intergenic
AGCTTTCTCATAAGGATCTG+AGG 0.541397 tig0012275:-104788 None:intergenic
GACACTTTGGACTCTAAGAA+TGG 0.544103 tig0012275:+105635 Msa1351480:three_prime_UTR
GTATATTGATGAGGTTTGGA+AGG 0.548215 tig0012275:+104747 Msa1351480:CDS
TGCAGGTTGGTGATGTTGGA+GGG 0.552099 tig0012275:+105282 Msa1351480:intron
TTGTTGTTGACTATAGGAAG+AGG 0.553068 tig0012275:+105594 Msa1351480:CDS
AGTGTGATAAACTGATTGCT+TGG 0.559583 tig0012275:+105504 Msa1351480:CDS
GTATGTTTGGTATGAGAGCA+AGG 0.566057 tig0012275:+104569 Msa1351480:CDS
GTGTGATAAACTGATTGCTT+GGG 0.572223 tig0012275:+105505 Msa1351480:CDS
CAGTGTTTCCAAATCTGTTG+CGG 0.573601 tig0012275:+105550 Msa1351480:CDS
GCTTTCTCATAAGGATCTGA+GGG 0.584199 tig0012275:-104787 None:intergenic
ATGCAGGTTGGTGATGTTGG+AGG 0.585807 tig0012275:+105281 Msa1351480:intron
GTTGGAGGGAGGATATGGGC+TGG 0.592056 tig0012275:+105296 Msa1351480:CDS
CATTGTTGAGTATATTGATG+AGG 0.596226 tig0012275:+104738 Msa1351480:CDS
TATGAGAGCAAGGATAGCAT+TGG 0.605598 tig0012275:+104579 Msa1351480:CDS
AACCACATAGCAACAAAGGA+AGG 0.613591 tig0012275:-105820 None:intergenic
TGTCACCACCAAAATAAGGT+TGG 0.621870 tig0012275:-105394 None:intergenic
GAAATTATGCAACTTCAAAG+TGG 0.628741 tig0012275:+105475 Msa1351480:CDS
TGAAAGATTTGAAAAGAACA+AGG 0.634058 tig0012275:-104488 None:intergenic
ATTATGCAACTTCAAAGTGG+AGG 0.642784 tig0012275:+105478 Msa1351480:CDS
TTGGAACCACATAGCAACAA+AGG 0.650782 tig0012275:-105824 None:intergenic
AGGTTGGTGATGTTGGAGGG+AGG 0.678043 tig0012275:+105285 Msa1351480:intron
TACCTGATTTAACTACGAGG+AGG 0.709862 tig0012275:+105882 Msa1351480:three_prime_UTR
TAGTACCTGATTTAACTACG+AGG 0.819381 tig0012275:+105879 Msa1351480:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATATGGTAAAAACTT+TGG - tig0012275:104867-104886 None:intergenic 15.0%
!! TAATGAATTAAATAACATGA+TGG + tig0012275:105002-105021 Msa1351480:intron 15.0%
!! TTAATATGTTAAAAAAATGC+AGG + tig0012275:105265-105284 Msa1351480:intron 15.0%
!! AAAATTTACACTTGAAAAAT+TGG + tig0012275:105792-105811 Msa1351480:three_prime_UTR 15.0%
!! AAATTTACACTTGAAAAATT+GGG + tig0012275:105793-105812 Msa1351480:three_prime_UTR 15.0%
!! CATTATACATATAACATATA+TGG - tig0012275:105910-105929 None:intergenic 15.0%
!! TATGTATAATGTATTTATGT+AGG + tig0012275:105919-105938 Msa1351480:three_prime_UTR 15.0%
!! TAAAAATATGGTAAAAACTT+TGG - tig0012275:104867-104886 None:intergenic 15.0%
!! TAATGAATTAAATAACATGA+TGG + tig0012275:105002-105021 Msa1351480:intron 15.0%
!! TTAATATGTTAAAAAAATGC+AGG + tig0012275:105265-105284 Msa1351480:intron 15.0%
!! AAAATTTACACTTGAAAAAT+TGG + tig0012275:105792-105811 Msa1351480:three_prime_UTR 15.0%
!! AAATTTACACTTGAAAAATT+GGG + tig0012275:105793-105812 Msa1351480:three_prime_UTR 15.0%
!! CATTATACATATAACATATA+TGG - tig0012275:105910-105929 None:intergenic 15.0%
!! TATGTATAATGTATTTATGT+AGG + tig0012275:105919-105938 Msa1351480:three_prime_UTR 15.0%
!! AGTTAATATTGTTAGATGAA+TGG + tig0012275:104542-104561 Msa1351480:CDS 20.0%
!! AGAGAAAACAATAAAAAAGA+AGG - tig0012275:105031-105050 None:intergenic 20.0%
!!! TTGTTTTCTCTAATATTCAA+TGG + tig0012275:105040-105059 Msa1351480:intron 20.0%
!! TATCCATATTATTAAAGACT+AGG + tig0012275:105200-105219 Msa1351480:intron 20.0%
!! TATAAAACCAACTATAGAAA+GGG - tig0012275:105448-105467 None:intergenic 20.0%
!! GAAATGTAAACTAAACAATT+TGG + tig0012275:105671-105690 Msa1351480:three_prime_UTR 20.0%
!! AGTTAATATTGTTAGATGAA+TGG + tig0012275:104542-104561 Msa1351480:CDS 20.0%
!! AGAGAAAACAATAAAAAAGA+AGG - tig0012275:105031-105050 None:intergenic 20.0%
!!! TTGTTTTCTCTAATATTCAA+TGG + tig0012275:105040-105059 Msa1351480:intron 20.0%
!! TATCCATATTATTAAAGACT+AGG + tig0012275:105200-105219 Msa1351480:intron 20.0%
!! TATAAAACCAACTATAGAAA+GGG - tig0012275:105448-105467 None:intergenic 20.0%
!! GAAATGTAAACTAAACAATT+TGG + tig0012275:105671-105690 Msa1351480:three_prime_UTR 20.0%
! TGAAAGATTTGAAAAGAACA+AGG - tig0012275:104491-104510 None:intergenic 25.0%
! AATATACTCAACAATGATGA+TGG - tig0012275:104734-104753 None:intergenic 25.0%
!! ATTTTTACTTATTTCCTGCT+AGG - tig0012275:104890-104909 None:intergenic 25.0%
!!! GTATTGTTTTTGCTTTTGAA+TGG + tig0012275:104967-104986 Msa1351480:intron 25.0%
! TTTCTCTAATATTCAATGGA+TGG + tig0012275:105044-105063 Msa1351480:intron 25.0%
! TACTATATTCATATCTCACT+AGG - tig0012275:105130-105149 None:intergenic 25.0%
! CTTCATATTTCATCAATGAA+TGG + tig0012275:105156-105175 Msa1351480:intron 25.0%
! GGATTTATCATTCATCTTAT+TGG + tig0012275:105177-105196 Msa1351480:intron 25.0%
!! CAACCTAGTCTTTAATAATA+TGG - tig0012275:105206-105225 None:intergenic 25.0%
! TATGTTAAAAAAATGCAGGT+TGG + tig0012275:105269-105288 Msa1351480:intron 25.0%
! GTATAAAACCAACTATAGAA+AGG - tig0012275:105449-105468 None:intergenic 25.0%
!!! ATGATTTTGTTGTTGACTAT+AGG + tig0012275:105588-105607 Msa1351480:CDS 25.0%
!! TAAGAATGGTATTGTTTTCT+TGG + tig0012275:105649-105668 Msa1351480:three_prime_UTR 25.0%
!! TAAATCAGGTACTATGTTTT+AGG - tig0012275:105873-105892 None:intergenic 25.0%
! TGAAAGATTTGAAAAGAACA+AGG - tig0012275:104491-104510 None:intergenic 25.0%
! AATATACTCAACAATGATGA+TGG - tig0012275:104734-104753 None:intergenic 25.0%
!! ATTTTTACTTATTTCCTGCT+AGG - tig0012275:104890-104909 None:intergenic 25.0%
!!! GTATTGTTTTTGCTTTTGAA+TGG + tig0012275:104967-104986 Msa1351480:intron 25.0%
! TTTCTCTAATATTCAATGGA+TGG + tig0012275:105044-105063 Msa1351480:intron 25.0%
! TACTATATTCATATCTCACT+AGG - tig0012275:105130-105149 None:intergenic 25.0%
! CTTCATATTTCATCAATGAA+TGG + tig0012275:105156-105175 Msa1351480:intron 25.0%
! GGATTTATCATTCATCTTAT+TGG + tig0012275:105177-105196 Msa1351480:intron 25.0%
!! CAACCTAGTCTTTAATAATA+TGG - tig0012275:105206-105225 None:intergenic 25.0%
! TATGTTAAAAAAATGCAGGT+TGG + tig0012275:105269-105288 Msa1351480:intron 25.0%
! GTATAAAACCAACTATAGAA+AGG - tig0012275:105449-105468 None:intergenic 25.0%
!!! ATGATTTTGTTGTTGACTAT+AGG + tig0012275:105588-105607 Msa1351480:CDS 25.0%
!! TAAGAATGGTATTGTTTTCT+TGG + tig0012275:105649-105668 Msa1351480:three_prime_UTR 25.0%
!! TAAATCAGGTACTATGTTTT+AGG - tig0012275:105873-105892 None:intergenic 25.0%
!!! TCTGTGATTGTTTTTACCAA+TGG + tig0012275:104513-104532 Msa1351480:exon 30.0%
!! GATTCATTTGCAAAAGCATT+TGG - tig0012275:104657-104676 None:intergenic 30.0%
ATTCCAGTTCTTATCCATAA+GGG + tig0012275:104691-104710 Msa1351480:CDS 30.0%
!! ATGATGATGGATTCACAAAT+TGG - tig0012275:104721-104740 None:intergenic 30.0%
CATTGTTGAGTATATTGATG+AGG + tig0012275:104738-104757 Msa1351480:CDS 30.0%
TTGAGTATATTGATGAGGTT+TGG + tig0012275:104743-104762 Msa1351480:CDS 30.0%
!!! ACAAGAAGGTAAGTTTTGTT+AGG + tig0012275:104839-104858 Msa1351480:intron 30.0%
!! TTACCATATTTTTACCTAGC+AGG + tig0012275:104873-104892 Msa1351480:intron 30.0%
TTTCCTGCTAGGTAAAAATA+TGG - tig0012275:104879-104898 None:intergenic 30.0%
ATATTCATATCTCACTAGGA+AGG - tig0012275:105126-105145 None:intergenic 30.0%
AAAGAGAGATGAGATTGAAT+TGG + tig0012275:105319-105338 Msa1351480:CDS 30.0%
GAAATTATGCAACTTCAAAG+TGG + tig0012275:105475-105494 Msa1351480:CDS 30.0%
! TATTTGACCAAAGCACTAAT+AGG - tig0012275:105702-105721 None:intergenic 30.0%
! TATAGTTATGCAAGTTTTGC+AGG + tig0012275:105750-105769 Msa1351480:three_prime_UTR 30.0%
!!! TCTGTGATTGTTTTTACCAA+TGG + tig0012275:104513-104532 Msa1351480:exon 30.0%
!! GATTCATTTGCAAAAGCATT+TGG - tig0012275:104657-104676 None:intergenic 30.0%
ATTCCAGTTCTTATCCATAA+GGG + tig0012275:104691-104710 Msa1351480:CDS 30.0%
!! ATGATGATGGATTCACAAAT+TGG - tig0012275:104721-104740 None:intergenic 30.0%
CATTGTTGAGTATATTGATG+AGG + tig0012275:104738-104757 Msa1351480:CDS 30.0%
TTGAGTATATTGATGAGGTT+TGG + tig0012275:104743-104762 Msa1351480:CDS 30.0%
!!! ACAAGAAGGTAAGTTTTGTT+AGG + tig0012275:104839-104858 Msa1351480:intron 30.0%
!! TTACCATATTTTTACCTAGC+AGG + tig0012275:104873-104892 Msa1351480:intron 30.0%
TTTCCTGCTAGGTAAAAATA+TGG - tig0012275:104879-104898 None:intergenic 30.0%
ATATTCATATCTCACTAGGA+AGG - tig0012275:105126-105145 None:intergenic 30.0%
AAAGAGAGATGAGATTGAAT+TGG + tig0012275:105319-105338 Msa1351480:CDS 30.0%
GAAATTATGCAACTTCAAAG+TGG + tig0012275:105475-105494 Msa1351480:CDS 30.0%
! TATTTGACCAAAGCACTAAT+AGG - tig0012275:105702-105721 None:intergenic 30.0%
! TATAGTTATGCAAGTTTTGC+AGG + tig0012275:105750-105769 Msa1351480:three_prime_UTR 30.0%
TTGAAAAGAACAAGGCTTTC+AGG - tig0012275:104483-104502 None:intergenic 35.0%
!!! ATTGTTTTTACCAATGGCAG+AGG + tig0012275:104519-104538 Msa1351480:exon 35.0%
AATATTAACTCCTCTGCCAT+TGG - tig0012275:104532-104551 None:intergenic 35.0%
GATGAATGGCTTAGTATGTT+TGG + tig0012275:104556-104575 Msa1351480:CDS 35.0%
AGGGATTAAGTATGAGTACA+AGG + tig0012275:104609-104628 Msa1351480:CDS 35.0%
ACTGGAATCTTCTTGTGAAT+TGG - tig0012275:104679-104698 None:intergenic 35.0%
GATTCCAGTTCTTATCCATA+AGG + tig0012275:104690-104709 Msa1351480:CDS 35.0%
TTTCCCTTATGGATAAGAAC+TGG - tig0012275:104697-104716 None:intergenic 35.0%
!!! CACAAATTGGTTTTCCCTTA+TGG - tig0012275:104708-104727 None:intergenic 35.0%
GTATATTGATGAGGTTTGGA+AGG + tig0012275:104747-104766 Msa1351480:CDS 35.0%
! AGAAAGCTCAAGCAAGATTT+TGG + tig0012275:104803-104822 Msa1351480:CDS 35.0%
! GAAAGCTCAAGCAAGATTTT+GGG + tig0012275:104804-104823 Msa1351480:CDS 35.0%
! GCAAAGAAAGAGTTGATTGA+TGG + tig0012275:105341-105360 Msa1351480:CDS 35.0%
! AGAGTTGATTGATGGCTTAA+AGG + tig0012275:105349-105368 Msa1351480:CDS 35.0%
AAGGAATTAGAGAATGTGCT+TGG + tig0012275:105368-105387 Msa1351480:CDS 35.0%
AATGTGTCACCACCAAAATA+AGG - tig0012275:105401-105420 None:intergenic 35.0%
!!! TATTTTGGTGGTGACACATT+TGG + tig0012275:105401-105420 Msa1351480:CDS 35.0%
!!! ATTTTGGTGGTGACACATTT+GGG + tig0012275:105402-105421 Msa1351480:CDS 35.0%
CTTTGATCCCTTTCTATAGT+TGG + tig0012275:105438-105457 Msa1351480:CDS 35.0%
ATTATGCAACTTCAAAGTGG+AGG + tig0012275:105478-105497 Msa1351480:CDS 35.0%
AGTGTGATAAACTGATTGCT+TGG + tig0012275:105504-105523 Msa1351480:CDS 35.0%
GTGTGATAAACTGATTGCTT+GGG + tig0012275:105505-105524 Msa1351480:CDS 35.0%
! TTGTTGTTGACTATAGGAAG+AGG + tig0012275:105594-105613 Msa1351480:CDS 35.0%
CTATAGGAAGAGGTTTGATT+TGG + tig0012275:105604-105623 Msa1351480:CDS 35.0%
! TTTAGGATCTGAGTACAGAT+TGG - tig0012275:105856-105875 None:intergenic 35.0%
TAGTACCTGATTTAACTACG+AGG + tig0012275:105879-105898 Msa1351480:three_prime_UTR 35.0%
TTGAAAAGAACAAGGCTTTC+AGG - tig0012275:104483-104502 None:intergenic 35.0%
!!! ATTGTTTTTACCAATGGCAG+AGG + tig0012275:104519-104538 Msa1351480:exon 35.0%
AATATTAACTCCTCTGCCAT+TGG - tig0012275:104532-104551 None:intergenic 35.0%
GATGAATGGCTTAGTATGTT+TGG + tig0012275:104556-104575 Msa1351480:CDS 35.0%
AGGGATTAAGTATGAGTACA+AGG + tig0012275:104609-104628 Msa1351480:CDS 35.0%
ACTGGAATCTTCTTGTGAAT+TGG - tig0012275:104679-104698 None:intergenic 35.0%
GATTCCAGTTCTTATCCATA+AGG + tig0012275:104690-104709 Msa1351480:CDS 35.0%
TTTCCCTTATGGATAAGAAC+TGG - tig0012275:104697-104716 None:intergenic 35.0%
!!! CACAAATTGGTTTTCCCTTA+TGG - tig0012275:104708-104727 None:intergenic 35.0%
GTATATTGATGAGGTTTGGA+AGG + tig0012275:104747-104766 Msa1351480:CDS 35.0%
! AGAAAGCTCAAGCAAGATTT+TGG + tig0012275:104803-104822 Msa1351480:CDS 35.0%
! GAAAGCTCAAGCAAGATTTT+GGG + tig0012275:104804-104823 Msa1351480:CDS 35.0%
! GCAAAGAAAGAGTTGATTGA+TGG + tig0012275:105341-105360 Msa1351480:CDS 35.0%
! AGAGTTGATTGATGGCTTAA+AGG + tig0012275:105349-105368 Msa1351480:CDS 35.0%
AAGGAATTAGAGAATGTGCT+TGG + tig0012275:105368-105387 Msa1351480:CDS 35.0%
AATGTGTCACCACCAAAATA+AGG - tig0012275:105401-105420 None:intergenic 35.0%
!!! TATTTTGGTGGTGACACATT+TGG + tig0012275:105401-105420 Msa1351480:CDS 35.0%
!!! ATTTTGGTGGTGACACATTT+GGG + tig0012275:105402-105421 Msa1351480:CDS 35.0%
CTTTGATCCCTTTCTATAGT+TGG + tig0012275:105438-105457 Msa1351480:CDS 35.0%
ATTATGCAACTTCAAAGTGG+AGG + tig0012275:105478-105497 Msa1351480:CDS 35.0%
AGTGTGATAAACTGATTGCT+TGG + tig0012275:105504-105523 Msa1351480:CDS 35.0%
GTGTGATAAACTGATTGCTT+GGG + tig0012275:105505-105524 Msa1351480:CDS 35.0%
! TTGTTGTTGACTATAGGAAG+AGG + tig0012275:105594-105613 Msa1351480:CDS 35.0%
CTATAGGAAGAGGTTTGATT+TGG + tig0012275:105604-105623 Msa1351480:CDS 35.0%
! TTTAGGATCTGAGTACAGAT+TGG - tig0012275:105856-105875 None:intergenic 35.0%
TAGTACCTGATTTAACTACG+AGG + tig0012275:105879-105898 Msa1351480:three_prime_UTR 35.0%
GTATGTTTGGTATGAGAGCA+AGG + tig0012275:104569-104588 Msa1351480:CDS 40.0%
TATGAGAGCAAGGATAGCAT+TGG + tig0012275:104579-104598 Msa1351480:CDS 40.0%
!! AGGATAGCATTGGCTGAAAA+AGG + tig0012275:104589-104608 Msa1351480:CDS 40.0%
!! GGATAGCATTGGCTGAAAAA+GGG + tig0012275:104590-104609 Msa1351480:CDS 40.0%
TGATGAGGTTTGGAAGGATA+AGG + tig0012275:104753-104772 Msa1351480:CDS 40.0%
GCTTTCTCATAAGGATCTGA+GGG - tig0012275:104790-104809 None:intergenic 40.0%
AGCTTTCTCATAAGGATCTG+AGG - tig0012275:104791-104810 None:intergenic 40.0%
CTTGCTTGAGCTTTCTCATA+AGG - tig0012275:104799-104818 None:intergenic 40.0%
!! GGCTGATTTTGTCAACAAGA+AGG + tig0012275:104825-104844 Msa1351480:CDS 40.0%
CATATCTCACTAGGAAGGAA+AGG - tig0012275:105121-105140 None:intergenic 40.0%
!! AAAATGCAGGTTGGTGATGT+TGG + tig0012275:105278-105297 Msa1351480:intron 40.0%
!! CTTGGAGACCAACCTTATTT+TGG + tig0012275:105386-105405 Msa1351480:CDS 40.0%
TGTCACCACCAAAATAAGGT+TGG - tig0012275:105397-105416 None:intergenic 40.0%
CAGTGTTTCCAAATCTGTTG+CGG + tig0012275:105550-105569 Msa1351480:CDS 40.0%
TTTCTCATCCGCAACAGATT+TGG - tig0012275:105561-105580 None:intergenic 40.0%
TTTGGACTGAGAAGACACTT+TGG + tig0012275:105622-105641 Msa1351480:exon 40.0%
! GACACTTTGGACTCTAAGAA+TGG + tig0012275:105635-105654 Msa1351480:three_prime_UTR 40.0%
TTCCTTCCTTTGTTGCTATG+TGG + tig0012275:105818-105837 Msa1351480:three_prime_UTR 40.0%
AACCACATAGCAACAAAGGA+AGG - tig0012275:105823-105842 None:intergenic 40.0%
TTGGAACCACATAGCAACAA+AGG - tig0012275:105827-105846 None:intergenic 40.0%
TACCTGATTTAACTACGAGG+AGG + tig0012275:105882-105901 Msa1351480:three_prime_UTR 40.0%
GTATGTTTGGTATGAGAGCA+AGG + tig0012275:104569-104588 Msa1351480:CDS 40.0%
TATGAGAGCAAGGATAGCAT+TGG + tig0012275:104579-104598 Msa1351480:CDS 40.0%
!! AGGATAGCATTGGCTGAAAA+AGG + tig0012275:104589-104608 Msa1351480:CDS 40.0%
!! GGATAGCATTGGCTGAAAAA+GGG + tig0012275:104590-104609 Msa1351480:CDS 40.0%
TGATGAGGTTTGGAAGGATA+AGG + tig0012275:104753-104772 Msa1351480:CDS 40.0%
GCTTTCTCATAAGGATCTGA+GGG - tig0012275:104790-104809 None:intergenic 40.0%
AGCTTTCTCATAAGGATCTG+AGG - tig0012275:104791-104810 None:intergenic 40.0%
CTTGCTTGAGCTTTCTCATA+AGG - tig0012275:104799-104818 None:intergenic 40.0%
!! GGCTGATTTTGTCAACAAGA+AGG + tig0012275:104825-104844 Msa1351480:CDS 40.0%
CATATCTCACTAGGAAGGAA+AGG - tig0012275:105121-105140 None:intergenic 40.0%
!! AAAATGCAGGTTGGTGATGT+TGG + tig0012275:105278-105297 Msa1351480:intron 40.0%
!! CTTGGAGACCAACCTTATTT+TGG + tig0012275:105386-105405 Msa1351480:CDS 40.0%
TGTCACCACCAAAATAAGGT+TGG - tig0012275:105397-105416 None:intergenic 40.0%
CAGTGTTTCCAAATCTGTTG+CGG + tig0012275:105550-105569 Msa1351480:CDS 40.0%
TTTCTCATCCGCAACAGATT+TGG - tig0012275:105561-105580 None:intergenic 40.0%
TTTGGACTGAGAAGACACTT+TGG + tig0012275:105622-105641 Msa1351480:exon 40.0%
! GACACTTTGGACTCTAAGAA+TGG + tig0012275:105635-105654 Msa1351480:three_prime_UTR 40.0%
TTCCTTCCTTTGTTGCTATG+TGG + tig0012275:105818-105837 Msa1351480:three_prime_UTR 40.0%
AACCACATAGCAACAAAGGA+AGG - tig0012275:105823-105842 None:intergenic 40.0%
TTGGAACCACATAGCAACAA+AGG - tig0012275:105827-105846 None:intergenic 40.0%
TACCTGATTTAACTACGAGG+AGG + tig0012275:105882-105901 Msa1351480:three_prime_UTR 40.0%
TGATGTTGGAGGGAGGATAT+GGG + tig0012275:105292-105311 Msa1351480:CDS 45.0%
!!! GGAGACCAACCTTATTTTGG+TGG + tig0012275:105389-105408 Msa1351480:CDS 45.0%
GGTTGCTCCTATTAGTGCTT+TGG + tig0012275:105692-105711 Msa1351480:three_prime_UTR 45.0%
GAGTACAGATTGGAGGTGAT+TGG - tig0012275:105846-105865 None:intergenic 45.0%
AGGATCTGAGTACAGATTGG+AGG - tig0012275:105853-105872 None:intergenic 45.0%
CTCCTCCTCGTAGTTAAATC+AGG - tig0012275:105887-105906 None:intergenic 45.0%
TGATGTTGGAGGGAGGATAT+GGG + tig0012275:105292-105311 Msa1351480:CDS 45.0%
!!! GGAGACCAACCTTATTTTGG+TGG + tig0012275:105389-105408 Msa1351480:CDS 45.0%
GGTTGCTCCTATTAGTGCTT+TGG + tig0012275:105692-105711 Msa1351480:three_prime_UTR 45.0%
GAGTACAGATTGGAGGTGAT+TGG - tig0012275:105846-105865 None:intergenic 45.0%
AGGATCTGAGTACAGATTGG+AGG - tig0012275:105853-105872 None:intergenic 45.0%
CTCCTCCTCGTAGTTAAATC+AGG - tig0012275:105887-105906 None:intergenic 45.0%
!! ATGCAGGTTGGTGATGTTGG+AGG + tig0012275:105281-105300 Msa1351480:intron 50.0%
!! TGCAGGTTGGTGATGTTGGA+GGG + tig0012275:105282-105301 Msa1351480:intron 50.0%
GTGATGTTGGAGGGAGGATA+TGG + tig0012275:105291-105310 Msa1351480:CDS 50.0%
!! ATGCAGGTTGGTGATGTTGG+AGG + tig0012275:105281-105300 Msa1351480:intron 50.0%
!! TGCAGGTTGGTGATGTTGGA+GGG + tig0012275:105282-105301 Msa1351480:intron 50.0%
GTGATGTTGGAGGGAGGATA+TGG + tig0012275:105291-105310 Msa1351480:CDS 50.0%
!! AGGTTGGTGATGTTGGAGGG+AGG + tig0012275:105285-105304 Msa1351480:intron 55.0%
!! AGGTTGGTGATGTTGGAGGG+AGG + tig0012275:105285-105304 Msa1351480:intron 55.0%
GTTGGAGGGAGGATATGGGC+TGG + tig0012275:105296-105315 Msa1351480:CDS 60.0%
GTTGGAGGGAGGATATGGGC+TGG + tig0012275:105296-105315 Msa1351480:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0012275 gene 104442 105962 104442 ID=Msa1351480;Name=Msa1351480
tig0012275 mRNA 104442 105962 104442 ID=Msa1351480-mRNA-1;Parent=Msa1351480;Name=Msa1351480-mRNA-1;_AED=0.05;_eAED=0.05;_QI=90|1|1|1|1|1|2|331|219
tig0012275 exon 104442 104846 104442 ID=Msa1351480-mRNA-1:exon:7733;Parent=Msa1351480-mRNA-1
tig0012275 exon 105287 105962 105287 ID=Msa1351480-mRNA-1:exon:7734;Parent=Msa1351480-mRNA-1
tig0012275 five_prime_UTR 104442 104531 104442 ID=Msa1351480-mRNA-1:five_prime_utr;Parent=Msa1351480-mRNA-1
tig0012275 CDS 104532 104846 104532 ID=Msa1351480-mRNA-1:cds;Parent=Msa1351480-mRNA-1
tig0012275 CDS 105287 105631 105287 ID=Msa1351480-mRNA-1:cds;Parent=Msa1351480-mRNA-1
tig0012275 three_prime_UTR 105632 105962 105632 ID=Msa1351480-mRNA-1:three_prime_utr;Parent=Msa1351480-mRNA-1
Gene Sequence

>Msa1351480

ATGGCAGAGGAGTTAATATTGTTAGATGAATGGCTTAGTATGTTTGGTATGAGAGCAAGGATAGCATTGGCTGAAAAAGGGATTAAGTATGAGTACAAGGAAGAAGATCTTGAAAACAAGAGCCAAATGCTTTTGCAAATGAATCCAATTCACAAGAAGATTCCAGTTCTTATCCATAAGGGAAAACCAATTTGTGAATCCATCATCATTGTTGAGTATATTGATGAGGTTTGGAAGGATAAGGTCTCATTTCTACCCTCAGATCCTTATGAGAAAGCTCAAGCAAGATTTTGGGCTGATTTTGTCAACAAGAAGGTTGGTGATGTTGGAGGGAGGATATGGGCTGGAAAGAGAGATGAGATTGAATTGGCAAAGAAAGAGTTGATTGATGGCTTAAAGGAATTAGAGAATGTGCTTGGAGACCAACCTTATTTTGGTGGTGACACATTTGGGTTTGTTGACATTGCTTTGATCCCTTTCTATAGTTGGTTTTATACTTATGAGAAATTATGCAACTTCAAAGTGGAGGAAGAGTGTGATAAACTGATTGCTTGGGCAAAGAGATGCAAGCAAAGAGACAGTGTTTCCAAATCTGTTGCGGATGAGAAAGAAGTGTATGATTTTGTTGTTGACTATAGGAAGAGGTTTGATTTGGACTGA
Protein sequence

>Msa1351480

MAEELILLDEWLSMFGMRARIALAEKGIKYEYKEEDLENKSQMLLQMNPIHKKIPVLIHKGKPICESIIIVEYIDEVWKDKVSFLPSDPYEKAQARFWADFVNKKVGDVGGRIWAGKRDEIELAKKELIDGLKELENVLGDQPYFGGDTFGFVDIALIPFYSWFYTYEKLCNFKVEEECDKLIAWAKRCKQRDSVSKSVADEKEVYDFVVDYRKRFDLD*