AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1285730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1285730 MtrunA17_Chr8g0357241 93.151 219 15 0 1 219 1 219 3.10e-151 419
Msa1285730 MtrunA17_Chr8g0357211 93.151 219 15 0 1 219 1 219 9.50e-151 417
Msa1285730 MtrunA17_Chr2g0312681 65.297 219 76 0 1 219 1 219 3.51e-105 302
Msa1285730 MtrunA17_Chr2g0312691 64.840 219 77 0 1 219 1 219 3.23e-103 297
Msa1285730 MtrunA17_Chr2g0312771 63.927 219 79 0 1 219 1 219 7.64e-102 293
Msa1285730 MtrunA17_Chr2g0312751 64.384 219 78 0 1 219 1 219 1.36e-101 293
Msa1285730 MtrunA17_Chr2g0312781 64.019 214 77 0 1 214 18 231 9.20e-100 289
Msa1285730 MtrunA17_Chr2g0312711 62.557 219 81 1 1 219 1 218 8.96e-96 278
Msa1285730 MtrunA17_Chr2g0312731 58.904 219 90 0 1 219 1 219 1.15e-95 278
Msa1285730 MtrunA17_Chr2g0312791 60.550 218 84 2 1 216 1 218 4.05e-92 269
Msa1285730 MtrunA17_Chr3g0107731 55.349 215 96 0 5 219 10 224 6.60e-91 266
Msa1285730 MtrunA17_Chr3g0107741 54.839 217 98 0 3 219 8 224 1.50e-90 265
Msa1285730 MtrunA17_Chr2g0312811 57.919 221 91 1 1 219 1 221 2.32e-90 265
Msa1285730 MtrunA17_Chr2g0312721 55.981 209 92 0 1 209 1 209 5.36e-82 243
Msa1285730 MtrunA17_Chr4g0069471 63.388 183 64 2 1 182 22 202 5.14e-80 238
Msa1285730 MtrunA17_Chr5g0433801 51.402 214 102 2 2 214 4 216 4.12e-72 218
Msa1285730 MtrunA17_Chr4g0029341 40.376 213 122 3 1 209 1 212 3.33e-44 147
Msa1285730 MtrunA17_Chr5g0415411 40.826 218 120 4 2 210 3 220 4.37e-44 147
Msa1285730 MtrunA17_Chr1g0196661 39.901 203 117 3 11 209 10 211 7.04e-44 146
Msa1285730 MtrunA17_Chr4g0008781 37.799 209 125 4 3 208 7 213 4.92e-43 144
Msa1285730 MtrunA17_Chr5g0417011 35.610 205 125 2 2 199 5 209 6.80e-43 144
Msa1285730 MtrunA17_Chr3g0132581 48.171 164 83 2 1 163 1 163 6.07e-42 141
Msa1285730 MtrunA17_Chr7g0240641 38.756 209 123 4 3 208 6 212 2.49e-41 140
Msa1285730 MtrunA17_Chr7g0240681 38.208 212 121 5 3 208 6 213 5.25e-41 139
Msa1285730 MtrunA17_Chr7g0240631 37.321 209 126 4 3 208 6 212 6.65e-41 139
Msa1285730 MtrunA17_Chr7g0240691 40.476 210 119 4 3 208 36 243 1.90e-40 139
Msa1285730 MtrunA17_Chr8g0379581 35.321 218 135 3 2 217 3 216 1.90e-40 137
Msa1285730 MtrunA17_Chr7g0240611 43.548 186 100 5 3 187 6 187 1.19e-39 135
Msa1285730 MtrunA17_Chr4g0008791 38.942 208 122 4 4 208 7 212 1.40e-39 135
Msa1285730 MtrunA17_Chr1g0196721 41.089 202 114 3 11 208 9 209 2.30e-39 135
Msa1285730 MtrunA17_Chr1g0196691 39.070 215 117 5 4 209 28 237 1.30e-38 134
Msa1285730 MtrunA17_Chr6g0483141 40.777 206 112 6 19 216 16 219 1.54e-38 132
Msa1285730 MtrunA17_Chr8g0363751 40.796 201 104 5 7 195 8 205 2.27e-38 132
Msa1285730 MtrunA17_Chr1g0196711 40.099 202 116 4 11 208 10 210 2.53e-37 129
Msa1285730 MtrunA17_Chr7g0240671 47.500 160 79 4 1 158 1 157 3.14e-37 129
Msa1285730 MtrunA17_Chr7g0240541 37.981 208 121 5 13 217 16 218 5.16e-37 129
Msa1285730 MtrunA17_Chr7g0240531 38.462 208 120 5 13 217 16 218 7.20e-37 128
Msa1285730 MtrunA17_Chr8g0379561 43.871 155 86 1 3 157 5 158 2.21e-36 127
Msa1285730 MtrunA17_Chr7g0240511 39.904 208 112 7 13 217 16 213 3.56e-36 126
Msa1285730 MtrunA17_Chr7g0240561 37.895 190 113 4 13 199 16 203 1.24e-35 125
Msa1285730 MtrunA17_Chr1g0196771 43.671 158 83 4 3 157 2 156 3.18e-35 124
Msa1285730 MtrunA17_Chr7g0240651 45.223 157 83 3 3 158 6 160 3.85e-35 124
Msa1285730 MtrunA17_Chr7g0240551 37.019 208 123 5 13 217 16 218 1.08e-34 123
Msa1285730 MtrunA17_Chr1g0212301 36.634 202 118 4 7 201 6 204 9.80e-34 120
Msa1285730 MtrunA17_Chr7g0240581 39.362 188 109 4 3 187 6 191 2.46e-33 119
Msa1285730 MtrunA17_Chr7g0240661 45.806 155 81 3 3 156 6 158 2.59e-33 119
Msa1285730 MtrunA17_Chr8g0391631 42.581 155 76 4 7 151 24 175 2.96e-32 115
Msa1285730 MtrunA17_Chr7g0240521 38.830 188 110 4 3 187 13 198 1.01e-31 115
Msa1285730 MtrunA17_Chr2g0313561 66.667 72 24 0 47 118 1 72 3.69e-30 106
Msa1285730 MtrunA17_Chr5g0415421 45.205 73 38 1 28 98 11 83 2.88e-12 61.6
Msa1285730 MtrunA17_Chr4g0076691 29.825 171 103 5 3 159 14 181 1.27e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1285730 AT1G78380.1 63.426 216 79 0 1 216 1 216 2.23e-97 283
Msa1285730 AT1G78370.1 60.000 215 86 0 1 215 1 215 3.32e-96 280
Msa1285730 AT1G17170.1 61.187 219 84 1 1 219 1 218 9.83e-96 278
Msa1285730 AT1G17180.1 59.817 219 88 0 1 219 1 219 1.66e-94 275
Msa1285730 AT1G78340.1 60.748 214 84 0 1 214 1 214 4.33e-94 274
Msa1285730 AT1G78360.1 60.465 215 84 1 1 214 1 215 1.04e-91 268
Msa1285730 AT1G78320.1 59.346 214 87 0 1 214 1 214 9.27e-89 261
Msa1285730 AT1G17190.1 55.607 214 95 0 3 216 4 217 1.21e-85 253
Msa1285730 AT1G53680.1 53.302 212 97 2 5 214 8 219 2.93e-81 242
Msa1285730 AT3G43800.1 51.643 213 99 3 3 211 4 216 1.22e-71 218
Msa1285730 AT1G78320.2 50.467 214 79 1 1 214 1 187 5.63e-68 207
Msa1285730 AT3G09270.1 47.872 188 93 4 3 186 5 191 1.57e-46 154
Msa1285730 AT2G29420.1 38.073 218 129 3 2 217 7 220 8.96e-46 152
Msa1285730 AT1G10370.1 39.352 216 123 4 2 209 3 218 3.24e-44 148
Msa1285730 AT1G10360.1 39.352 216 123 4 2 209 3 218 3.75e-43 145
Msa1285730 AT1G74590.1 35.000 220 131 4 5 215 8 224 2.73e-42 143
Msa1285730 AT1G59670.1 41.071 224 123 5 3 217 5 228 8.41e-42 142
Msa1285730 AT2G29460.1 39.908 218 125 4 3 216 6 221 1.32e-41 141
Msa1285730 AT2G29450.1 49.383 162 77 3 3 160 5 165 4.34e-41 139
Msa1285730 AT2G29440.1 47.205 161 81 2 3 160 5 164 8.69e-41 139
Msa1285730 AT1G59700.1 40.278 216 120 5 3 209 5 220 8.95e-41 139
Msa1285730 AT2G29490.1 45.342 161 84 2 3 160 6 165 1.43e-40 138
Msa1285730 AT2G29480.1 44.099 161 86 2 3 160 6 165 4.33e-40 137
Msa1285730 AT2G29470.1 37.037 216 129 5 7 217 10 223 9.92e-40 136
Msa1285730 AT1G27140.1 38.967 213 117 5 15 217 17 226 6.07e-39 135
Msa1285730 AT5G62480.1 37.363 182 100 2 5 175 9 187 3.71e-38 132
Msa1285730 AT1G27130.1 37.264 212 124 4 7 209 9 220 5.92e-38 132
Msa1285730 AT1G69920.1 36.916 214 122 5 7 209 37 248 4.43e-37 130
Msa1285730 AT1G69930.1 34.762 210 130 3 7 209 15 224 7.99e-35 124
Msa1285730 AT5G62480.3 29.121 182 88 3 5 175 9 160 7.63e-21 87.0
Msa1285730 AT5G62480.2 29.121 182 89 3 5 175 9 161 9.91e-21 86.7

Find 65 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTTAAACTAAATAATT+TGG 0.150487 8_3:+31404361 Msa1285730:three_prime_UTR
GATGAATGGCTTAGTATATT+TGG 0.159418 8_3:+31403279 Msa1285730:CDS
AGAAAGCTCAAGCAAGATTT+TGG 0.160142 8_3:+31403526 Msa1285730:CDS
ATGATGATGGATTCACAAAT+TGG 0.232208 8_3:-31403441 None:intergenic
TATAATTCGATCACGTTATA+TGG 0.232698 8_3:-31404594 None:intergenic
AGGATAGCATTGGCTGAAAA+AGG 0.234872 8_3:+31403312 Msa1285730:CDS
CTTGGAGACCAACCTTATTT+TGG 0.247910 8_3:+31404065 Msa1285730:CDS
GGAGTTGATTGAAGGCTTAA+AGG 0.261757 8_3:+31404028 Msa1285730:CDS
ACTGGAATCTTCTTGTGAAT+TGG 0.280000 8_3:-31403399 None:intergenic
TTTCTCATCAGCAACAGATT+TGG 0.313491 8_3:-31404237 None:intergenic
GATTCATTTGCAAAAGCATT+TGG 0.343663 8_3:-31403377 None:intergenic
GTGATGTTGGAGGAAGGATA+TGG 0.346197 8_3:+31403970 Msa1285730:CDS
CTTGCTTGAGCTTTCTCATA+AGG 0.355050 8_3:-31403519 None:intergenic
TATTAGACAAAAGCACTAAT+AGG 0.363163 8_3:-31404389 None:intergenic
TGATTTAGATCGAAGGTGAT+TGG 0.372369 8_3:-31404503 None:intergenic
AAAGAGAGAAAAGGTTGAAT+TGG 0.382179 8_3:+31403998 Msa1285730:CDS
TTATAACAAAGAAGAACTTA+TGG 0.398932 8_3:+31404612 Msa1285730:three_prime_UTR
TTTCCCTTATGGATAAGAAC+TGG 0.413273 8_3:-31403417 None:intergenic
GATTCCAGTTCTTATCCATA+AGG 0.427078 8_3:+31403413 Msa1285730:CDS
TTGAGTATATTGATGAGGTT+TGG 0.428745 8_3:+31403466 Msa1285730:CDS
ATGAATTTGTTGTTAACTAT+AGG 0.433226 8_3:+31404267 Msa1285730:CDS
AATGTGTCACCACCAAAATA+AGG 0.439985 8_3:-31404077 None:intergenic
TGATGAGGTTTGGAAGGATA+AGG 0.440755 8_3:+31403476 Msa1285730:CDS
TCTGTGGTTGTCTTTGACAA+TGG 0.448156 8_3:+31403236 Msa1285730:exon
CTAGTTGTTTACAATTCTCT+AGG 0.450049 8_3:+31403212 Msa1285730:five_prime_UTR
GTAAAGAAGGAGTTGATTGA+AGG 0.450966 8_3:+31404020 Msa1285730:CDS
TATAAAACCAACTATGAAAA+GGG 0.461833 8_3:-31404124 None:intergenic
ATTCCAGTTCTTATCCATAA+GGG 0.462545 8_3:+31403414 Msa1285730:CDS
GTATAAAACCAACTATGAAA+AGG 0.463905 8_3:-31404125 None:intergenic
AGTTGATATTACTTGATGAA+TGG 0.465432 8_3:+31403265 Msa1285730:CDS
AAAGGTTGAATTGGTAAAGA+AGG 0.468481 8_3:+31404007 Msa1285730:CDS
AAAATGCAGGTTGGTGATGT+TGG 0.481244 8_3:+31403957 Msa1285730:intron
CTCCTCCTCGTAGTTAAGTA+AGG 0.482407 8_3:-31404571 None:intergenic
TGATGTTGGAGGAAGGATAT+GGG 0.485671 8_3:+31403971 Msa1285730:CDS
AATATACTCAACAATGATGA+TGG 0.489877 8_3:-31403454 None:intergenic
AAGGAATTAGAGAATGTGCT+TGG 0.497037 8_3:+31404047 Msa1285730:CDS
ATTGTACACAGGGTTTATGT+AGG 0.506181 8_3:-31404529 None:intergenic
CTATAGGAAGAGGTTAGAGT+TGG 0.508025 8_3:+31404283 Msa1285730:CDS
AGGGATAAAGTATGAGTACA+AGG 0.524364 8_3:+31403332 Msa1285730:CDS
AGCTTTCTCATAAGGATCTG+AGG 0.541397 8_3:-31403511 None:intergenic
TTGTTGTTAACTATAGGAAG+AGG 0.543349 8_3:+31404273 Msa1285730:CDS
ATTATGCAACTTCAAAGTTG+AGG 0.546513 8_3:+31404157 Msa1285730:CDS
GTTGGACTGAGAAGACACTT+TGG 0.547128 8_3:+31404301 Msa1285730:exon
GTATATTGATGAGGTTTGGA+AGG 0.548215 8_3:+31403470 Msa1285730:CDS
GGCAAAGAGATGCAAGCAAA+GGG 0.548622 8_3:+31404205 Msa1285730:CDS
GTTGGAGGAAGGATATGGGC+TGG 0.551186 8_3:+31403975 Msa1285730:CDS
TTAGGTTGTGATTGTACACA+GGG 0.552796 8_3:-31404539 None:intergenic
GTATATTTGGTATGAGAGCA+AGG 0.566575 8_3:+31403292 Msa1285730:CDS
TAGGATTTGATTTAGATCGA+AGG 0.567975 8_3:-31404510 None:intergenic
ATGGGCTGGAAAGAGAGAAA+AGG 0.570427 8_3:+31403989 Msa1285730:CDS
TTTAGGTTGTGATTGTACAC+AGG 0.575737 8_3:-31404540 None:intergenic
GCTTTCTCATAAGGATCTGA+GGG 0.584199 8_3:-31403510 None:intergenic
TTACAATTCTCTAGGCTCTG+TGG 0.586400 8_3:+31403220 Msa1285730:five_prime_UTR
CATTGTTGAGTATATTGATG+AGG 0.596226 8_3:+31403461 Msa1285730:CDS
TTGGAACCTCGTGGCAACAA+AGG 0.602959 8_3:-31404484 None:intergenic
ATGCAGGTTGGTGATGTTGG+AGG 0.604533 8_3:+31403960 Msa1285730:intron
GTTGTCTTTGACAATGGCAG+AGG 0.605201 8_3:+31403242 Msa1285730:exon
TATGAGAGCAAGGATAGCAT+TGG 0.605598 8_3:+31403302 Msa1285730:CDS
TTCTTTCCTTTGTTGCCACG+AGG 0.608236 8_3:+31404478 Msa1285730:three_prime_UTR
GGGCAAAGAGATGCAAGCAA+AGG 0.618800 8_3:+31404204 Msa1285730:CDS
TGTCACCACCAAAATAAGGT+TGG 0.621870 8_3:-31404073 None:intergenic
TACCTTACTTAACTACGAGG+AGG 0.652824 8_3:+31404569 Msa1285730:three_prime_UTR
AGGTTGGTGATGTTGGAGGA+AGG 0.655636 8_3:+31403964 Msa1285730:intron
CGAAGGTGATTGGAACCTCG+TGG 0.686676 8_3:-31404493 None:intergenic
TTGTACCTTACTTAACTACG+AGG 0.739581 8_3:+31404566 Msa1285730:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAATTAAAAAACATGA+TGG + chr8_3:31403687-31403706 Msa1285730:intron 10.0%
!! TAATTAATTAAAAAACATGA+TGG + chr8_3:31403687-31403706 Msa1285730:intron 10.0%
!! TTAATATGTTAAAAAAATGC+AGG + chr8_3:31403944-31403963 Msa1285730:intron 15.0%
!! AAAATTTACACTTGAAAAAT+TGG + chr8_3:31404452-31404471 Msa1285730:three_prime_UTR 15.0%
!! AAATTTACACTTGAAAAATT+GGG + chr8_3:31404453-31404472 Msa1285730:three_prime_UTR 15.0%
!! TTAATATGTTAAAAAAATGC+AGG + chr8_3:31403944-31403963 Msa1285730:intron 15.0%
!! AAAATTTACACTTGAAAAAT+TGG + chr8_3:31404452-31404471 Msa1285730:three_prime_UTR 15.0%
!! AAATTTACACTTGAAAAATT+GGG + chr8_3:31404453-31404472 Msa1285730:three_prime_UTR 15.0%
!! AATATGTTAAACCATTGAAT+GGG - chr8_3:31403606-31403625 None:intergenic 20.0%
!! AAATATGTTAAACCATTGAA+TGG - chr8_3:31403607-31403626 None:intergenic 20.0%
!! TATAAAACCAACTATGAAAA+GGG - chr8_3:31404127-31404146 None:intergenic 20.0%
!!! ATGAATTTGTTGTTAACTAT+AGG + chr8_3:31404267-31404286 Msa1285730:CDS 20.0%
!!! TATAGTTATGCAAATTTTGT+AGG + chr8_3:31404410-31404429 Msa1285730:three_prime_UTR 20.0%
!! TTATAACAAAGAAGAACTTA+TGG + chr8_3:31404612-31404631 Msa1285730:three_prime_UTR 20.0%
!! AATATGTTAAACCATTGAAT+GGG - chr8_3:31403606-31403625 None:intergenic 20.0%
!! AAATATGTTAAACCATTGAA+TGG - chr8_3:31403607-31403626 None:intergenic 20.0%
!! TATAAAACCAACTATGAAAA+GGG - chr8_3:31404127-31404146 None:intergenic 20.0%
!!! ATGAATTTGTTGTTAACTAT+AGG + chr8_3:31404267-31404286 Msa1285730:CDS 20.0%
!!! TATAGTTATGCAAATTTTGT+AGG + chr8_3:31404410-31404429 Msa1285730:three_prime_UTR 20.0%
!! TTATAACAAAGAAGAACTTA+TGG + chr8_3:31404612-31404631 Msa1285730:three_prime_UTR 20.0%
!! AGTTGATATTACTTGATGAA+TGG + chr8_3:31403265-31403284 Msa1285730:CDS 25.0%
! AATATACTCAACAATGATGA+TGG - chr8_3:31403457-31403476 None:intergenic 25.0%
!!! GTATTGTTTTTGCTTTTGAA+TGG + chr8_3:31403652-31403671 Msa1285730:intron 25.0%
! TAGAGAAGACAATAAATAGA+AGG - chr8_3:31403716-31403735 None:intergenic 25.0%
! TTGTCTTCTCTAATATTCAA+TGG + chr8_3:31403724-31403743 Msa1285730:intron 25.0%
! ACATTCTTCATATTTCGTAA+TGG + chr8_3:31403831-31403850 Msa1285730:intron 25.0%
! GATCCATATTAATAAAGACT+AGG + chr8_3:31403879-31403898 Msa1285730:intron 25.0%
!! CAACCTAGTCTTTATTAATA+TGG - chr8_3:31403885-31403904 None:intergenic 25.0%
! TATGTTAAAAAAATGCAGGT+TGG + chr8_3:31403948-31403967 Msa1285730:intron 25.0%
! GTATAAAACCAACTATGAAA+AGG - chr8_3:31404128-31404147 None:intergenic 25.0%
!!! TATTAGACAAAAGCACTAAT+AGG - chr8_3:31404392-31404411 None:intergenic 25.0%
!! TAAGTAAGGTACAATGTTTT+AGG - chr8_3:31404560-31404579 None:intergenic 25.0%
! TATAATTCGATCACGTTATA+TGG - chr8_3:31404597-31404616 None:intergenic 25.0%
!! AGTTGATATTACTTGATGAA+TGG + chr8_3:31403265-31403284 Msa1285730:CDS 25.0%
! AATATACTCAACAATGATGA+TGG - chr8_3:31403457-31403476 None:intergenic 25.0%
!!! GTATTGTTTTTGCTTTTGAA+TGG + chr8_3:31403652-31403671 Msa1285730:intron 25.0%
! TAGAGAAGACAATAAATAGA+AGG - chr8_3:31403716-31403735 None:intergenic 25.0%
! TTGTCTTCTCTAATATTCAA+TGG + chr8_3:31403724-31403743 Msa1285730:intron 25.0%
! ACATTCTTCATATTTCGTAA+TGG + chr8_3:31403831-31403850 Msa1285730:intron 25.0%
! GATCCATATTAATAAAGACT+AGG + chr8_3:31403879-31403898 Msa1285730:intron 25.0%
!! CAACCTAGTCTTTATTAATA+TGG - chr8_3:31403885-31403904 None:intergenic 25.0%
! TATGTTAAAAAAATGCAGGT+TGG + chr8_3:31403948-31403967 Msa1285730:intron 25.0%
! GTATAAAACCAACTATGAAA+AGG - chr8_3:31404128-31404147 None:intergenic 25.0%
!!! TATTAGACAAAAGCACTAAT+AGG - chr8_3:31404392-31404411 None:intergenic 25.0%
!! TAAGTAAGGTACAATGTTTT+AGG - chr8_3:31404560-31404579 None:intergenic 25.0%
! TATAATTCGATCACGTTATA+TGG - chr8_3:31404597-31404616 None:intergenic 25.0%
CTAGTTGTTTACAATTCTCT+AGG + chr8_3:31403212-31403231 Msa1285730:five_prime_UTR 30.0%
GATGAATGGCTTAGTATATT+TGG + chr8_3:31403279-31403298 Msa1285730:CDS 30.0%
!! GATTCATTTGCAAAAGCATT+TGG - chr8_3:31403380-31403399 None:intergenic 30.0%
ATTCCAGTTCTTATCCATAA+GGG + chr8_3:31403414-31403433 Msa1285730:CDS 30.0%
!! ATGATGATGGATTCACAAAT+TGG - chr8_3:31403444-31403463 None:intergenic 30.0%
CATTGTTGAGTATATTGATG+AGG + chr8_3:31403461-31403480 Msa1285730:CDS 30.0%
TTGAGTATATTGATGAGGTT+TGG + chr8_3:31403466-31403485 Msa1285730:CDS 30.0%
CTTCTCTAATATTCAATGGA+TGG + chr8_3:31403728-31403747 Msa1285730:intron 30.0%
! TCTTCATATTTCGTAATGGA+TGG + chr8_3:31403835-31403854 Msa1285730:intron 30.0%
AAAGAGAGAAAAGGTTGAAT+TGG + chr8_3:31403998-31404017 Msa1285730:CDS 30.0%
AAAGGTTGAATTGGTAAAGA+AGG + chr8_3:31404007-31404026 Msa1285730:CDS 30.0%
ATTATGCAACTTCAAAGTTG+AGG + chr8_3:31404157-31404176 Msa1285730:CDS 30.0%
AGTGTGAAAAACTGATTGTT+TGG + chr8_3:31404183-31404202 Msa1285730:CDS 30.0%
GTGTGAAAAACTGATTGTTT+GGG + chr8_3:31404184-31404203 Msa1285730:CDS 30.0%
! TTGTTGTTAACTATAGGAAG+AGG + chr8_3:31404273-31404292 Msa1285730:CDS 30.0%
! TAGGATTTGATTTAGATCGA+AGG - chr8_3:31404513-31404532 None:intergenic 30.0%
CTAGTTGTTTACAATTCTCT+AGG + chr8_3:31403212-31403231 Msa1285730:five_prime_UTR 30.0%
GATGAATGGCTTAGTATATT+TGG + chr8_3:31403279-31403298 Msa1285730:CDS 30.0%
!! GATTCATTTGCAAAAGCATT+TGG - chr8_3:31403380-31403399 None:intergenic 30.0%
ATTCCAGTTCTTATCCATAA+GGG + chr8_3:31403414-31403433 Msa1285730:CDS 30.0%
!! ATGATGATGGATTCACAAAT+TGG - chr8_3:31403444-31403463 None:intergenic 30.0%
CATTGTTGAGTATATTGATG+AGG + chr8_3:31403461-31403480 Msa1285730:CDS 30.0%
TTGAGTATATTGATGAGGTT+TGG + chr8_3:31403466-31403485 Msa1285730:CDS 30.0%
CTTCTCTAATATTCAATGGA+TGG + chr8_3:31403728-31403747 Msa1285730:intron 30.0%
! TCTTCATATTTCGTAATGGA+TGG + chr8_3:31403835-31403854 Msa1285730:intron 30.0%
AAAGAGAGAAAAGGTTGAAT+TGG + chr8_3:31403998-31404017 Msa1285730:CDS 30.0%
AAAGGTTGAATTGGTAAAGA+AGG + chr8_3:31404007-31404026 Msa1285730:CDS 30.0%
ATTATGCAACTTCAAAGTTG+AGG + chr8_3:31404157-31404176 Msa1285730:CDS 30.0%
AGTGTGAAAAACTGATTGTT+TGG + chr8_3:31404183-31404202 Msa1285730:CDS 30.0%
GTGTGAAAAACTGATTGTTT+GGG + chr8_3:31404184-31404203 Msa1285730:CDS 30.0%
! TTGTTGTTAACTATAGGAAG+AGG + chr8_3:31404273-31404292 Msa1285730:CDS 30.0%
! TAGGATTTGATTTAGATCGA+AGG - chr8_3:31404513-31404532 None:intergenic 30.0%
GTATATTTGGTATGAGAGCA+AGG + chr8_3:31403292-31403311 Msa1285730:CDS 35.0%
AGGGATAAAGTATGAGTACA+AGG + chr8_3:31403332-31403351 Msa1285730:CDS 35.0%
ACTGGAATCTTCTTGTGAAT+TGG - chr8_3:31403402-31403421 None:intergenic 35.0%
GATTCCAGTTCTTATCCATA+AGG + chr8_3:31403413-31403432 Msa1285730:CDS 35.0%
TTTCCCTTATGGATAAGAAC+TGG - chr8_3:31403420-31403439 None:intergenic 35.0%
!!! CACAAATTGGTTTTCCCTTA+TGG - chr8_3:31403431-31403450 None:intergenic 35.0%
GTATATTGATGAGGTTTGGA+AGG + chr8_3:31403470-31403489 Msa1285730:CDS 35.0%
! AGAAAGCTCAAGCAAGATTT+TGG + chr8_3:31403526-31403545 Msa1285730:CDS 35.0%
! GAAAGCTCAAGCAAGATTTT+GGG + chr8_3:31403527-31403546 Msa1285730:CDS 35.0%
GAATGGGACACAAAAAACTT+TGG - chr8_3:31403590-31403609 None:intergenic 35.0%
!!! GTTTTTTGTGTCCCATTCAA+TGG + chr8_3:31403592-31403611 Msa1285730:intron 35.0%
ATAGTCATATCTCACAAGGA+AGG - chr8_3:31403806-31403825 None:intergenic 35.0%
TCCTTGTGAGATATGACTAT+AGG + chr8_3:31403806-31403825 Msa1285730:intron 35.0%
TCCTATAGTCATATCTCACA+AGG - chr8_3:31403810-31403829 None:intergenic 35.0%
! GTAAAGAAGGAGTTGATTGA+AGG + chr8_3:31404020-31404039 Msa1285730:CDS 35.0%
AAGGAATTAGAGAATGTGCT+TGG + chr8_3:31404047-31404066 Msa1285730:CDS 35.0%
AATGTGTCACCACCAAAATA+AGG - chr8_3:31404080-31404099 None:intergenic 35.0%
!!! TATTTTGGTGGTGACACATT+TGG + chr8_3:31404080-31404099 Msa1285730:CDS 35.0%
!!! ATTTTGGTGGTGACACATTT+GGG + chr8_3:31404081-31404100 Msa1285730:CDS 35.0%
! CTTTGATCCCTTTTCATAGT+TGG + chr8_3:31404117-31404136 Msa1285730:CDS 35.0%
TTTCTCATCAGCAACAGATT+TGG - chr8_3:31404240-31404259 None:intergenic 35.0%
TGATTTAGATCGAAGGTGAT+TGG - chr8_3:31404506-31404525 None:intergenic 35.0%
ATTGTACACAGGGTTTATGT+AGG - chr8_3:31404532-31404551 None:intergenic 35.0%
! TTAGGTTGTGATTGTACACA+GGG - chr8_3:31404542-31404561 None:intergenic 35.0%
!! TTTAGGTTGTGATTGTACAC+AGG - chr8_3:31404543-31404562 None:intergenic 35.0%
TTGTACCTTACTTAACTACG+AGG + chr8_3:31404566-31404585 Msa1285730:three_prime_UTR 35.0%
GTATATTTGGTATGAGAGCA+AGG + chr8_3:31403292-31403311 Msa1285730:CDS 35.0%
AGGGATAAAGTATGAGTACA+AGG + chr8_3:31403332-31403351 Msa1285730:CDS 35.0%
ACTGGAATCTTCTTGTGAAT+TGG - chr8_3:31403402-31403421 None:intergenic 35.0%
GATTCCAGTTCTTATCCATA+AGG + chr8_3:31403413-31403432 Msa1285730:CDS 35.0%
TTTCCCTTATGGATAAGAAC+TGG - chr8_3:31403420-31403439 None:intergenic 35.0%
!!! CACAAATTGGTTTTCCCTTA+TGG - chr8_3:31403431-31403450 None:intergenic 35.0%
GTATATTGATGAGGTTTGGA+AGG + chr8_3:31403470-31403489 Msa1285730:CDS 35.0%
! AGAAAGCTCAAGCAAGATTT+TGG + chr8_3:31403526-31403545 Msa1285730:CDS 35.0%
! GAAAGCTCAAGCAAGATTTT+GGG + chr8_3:31403527-31403546 Msa1285730:CDS 35.0%
GAATGGGACACAAAAAACTT+TGG - chr8_3:31403590-31403609 None:intergenic 35.0%
!!! GTTTTTTGTGTCCCATTCAA+TGG + chr8_3:31403592-31403611 Msa1285730:intron 35.0%
ATAGTCATATCTCACAAGGA+AGG - chr8_3:31403806-31403825 None:intergenic 35.0%
TCCTTGTGAGATATGACTAT+AGG + chr8_3:31403806-31403825 Msa1285730:intron 35.0%
TCCTATAGTCATATCTCACA+AGG - chr8_3:31403810-31403829 None:intergenic 35.0%
! GTAAAGAAGGAGTTGATTGA+AGG + chr8_3:31404020-31404039 Msa1285730:CDS 35.0%
AAGGAATTAGAGAATGTGCT+TGG + chr8_3:31404047-31404066 Msa1285730:CDS 35.0%
AATGTGTCACCACCAAAATA+AGG - chr8_3:31404080-31404099 None:intergenic 35.0%
!!! TATTTTGGTGGTGACACATT+TGG + chr8_3:31404080-31404099 Msa1285730:CDS 35.0%
!!! ATTTTGGTGGTGACACATTT+GGG + chr8_3:31404081-31404100 Msa1285730:CDS 35.0%
! CTTTGATCCCTTTTCATAGT+TGG + chr8_3:31404117-31404136 Msa1285730:CDS 35.0%
TTTCTCATCAGCAACAGATT+TGG - chr8_3:31404240-31404259 None:intergenic 35.0%
TGATTTAGATCGAAGGTGAT+TGG - chr8_3:31404506-31404525 None:intergenic 35.0%
ATTGTACACAGGGTTTATGT+AGG - chr8_3:31404532-31404551 None:intergenic 35.0%
! TTAGGTTGTGATTGTACACA+GGG - chr8_3:31404542-31404561 None:intergenic 35.0%
!! TTTAGGTTGTGATTGTACAC+AGG - chr8_3:31404543-31404562 None:intergenic 35.0%
TTGTACCTTACTTAACTACG+AGG + chr8_3:31404566-31404585 Msa1285730:three_prime_UTR 35.0%
TTACAATTCTCTAGGCTCTG+TGG + chr8_3:31403220-31403239 Msa1285730:five_prime_UTR 40.0%
TCTGTGGTTGTCTTTGACAA+TGG + chr8_3:31403236-31403255 Msa1285730:exon 40.0%
TATGAGAGCAAGGATAGCAT+TGG + chr8_3:31403302-31403321 Msa1285730:CDS 40.0%
!! AGGATAGCATTGGCTGAAAA+AGG + chr8_3:31403312-31403331 Msa1285730:CDS 40.0%
!! GGATAGCATTGGCTGAAAAA+GGG + chr8_3:31403313-31403332 Msa1285730:CDS 40.0%
TGATGAGGTTTGGAAGGATA+AGG + chr8_3:31403476-31403495 Msa1285730:CDS 40.0%
GCTTTCTCATAAGGATCTGA+GGG - chr8_3:31403513-31403532 None:intergenic 40.0%
AGCTTTCTCATAAGGATCTG+AGG - chr8_3:31403514-31403533 None:intergenic 40.0%
CTTGCTTGAGCTTTCTCATA+AGG - chr8_3:31403522-31403541 None:intergenic 40.0%
!! GGCTGATTTTGTCAACAAGA+AGG + chr8_3:31403548-31403567 Msa1285730:CDS 40.0%
CATATCTCACAAGGAAGGAA+AGG - chr8_3:31403801-31403820 None:intergenic 40.0%
!! AAAATGCAGGTTGGTGATGT+TGG + chr8_3:31403957-31403976 Msa1285730:intron 40.0%
TGATGTTGGAGGAAGGATAT+GGG + chr8_3:31403971-31403990 Msa1285730:CDS 40.0%
! GGAGTTGATTGAAGGCTTAA+AGG + chr8_3:31404028-31404047 Msa1285730:CDS 40.0%
!! CTTGGAGACCAACCTTATTT+TGG + chr8_3:31404065-31404084 Msa1285730:CDS 40.0%
TGTCACCACCAAAATAAGGT+TGG - chr8_3:31404076-31404095 None:intergenic 40.0%
CTATAGGAAGAGGTTAGAGT+TGG + chr8_3:31404283-31404302 Msa1285730:CDS 40.0%
TACCTTACTTAACTACGAGG+AGG + chr8_3:31404569-31404588 Msa1285730:three_prime_UTR 40.0%
TTACAATTCTCTAGGCTCTG+TGG + chr8_3:31403220-31403239 Msa1285730:five_prime_UTR 40.0%
TCTGTGGTTGTCTTTGACAA+TGG + chr8_3:31403236-31403255 Msa1285730:exon 40.0%
TATGAGAGCAAGGATAGCAT+TGG + chr8_3:31403302-31403321 Msa1285730:CDS 40.0%
!! AGGATAGCATTGGCTGAAAA+AGG + chr8_3:31403312-31403331 Msa1285730:CDS 40.0%
!! GGATAGCATTGGCTGAAAAA+GGG + chr8_3:31403313-31403332 Msa1285730:CDS 40.0%
TGATGAGGTTTGGAAGGATA+AGG + chr8_3:31403476-31403495 Msa1285730:CDS 40.0%
GCTTTCTCATAAGGATCTGA+GGG - chr8_3:31403513-31403532 None:intergenic 40.0%
AGCTTTCTCATAAGGATCTG+AGG - chr8_3:31403514-31403533 None:intergenic 40.0%
CTTGCTTGAGCTTTCTCATA+AGG - chr8_3:31403522-31403541 None:intergenic 40.0%
!! GGCTGATTTTGTCAACAAGA+AGG + chr8_3:31403548-31403567 Msa1285730:CDS 40.0%
CATATCTCACAAGGAAGGAA+AGG - chr8_3:31403801-31403820 None:intergenic 40.0%
!! AAAATGCAGGTTGGTGATGT+TGG + chr8_3:31403957-31403976 Msa1285730:intron 40.0%
TGATGTTGGAGGAAGGATAT+GGG + chr8_3:31403971-31403990 Msa1285730:CDS 40.0%
! GGAGTTGATTGAAGGCTTAA+AGG + chr8_3:31404028-31404047 Msa1285730:CDS 40.0%
!! CTTGGAGACCAACCTTATTT+TGG + chr8_3:31404065-31404084 Msa1285730:CDS 40.0%
TGTCACCACCAAAATAAGGT+TGG - chr8_3:31404076-31404095 None:intergenic 40.0%
CTATAGGAAGAGGTTAGAGT+TGG + chr8_3:31404283-31404302 Msa1285730:CDS 40.0%
TACCTTACTTAACTACGAGG+AGG + chr8_3:31404569-31404588 Msa1285730:three_prime_UTR 40.0%
GTTGTCTTTGACAATGGCAG+AGG + chr8_3:31403242-31403261 Msa1285730:exon 45.0%
GTGATGTTGGAGGAAGGATA+TGG + chr8_3:31403970-31403989 Msa1285730:CDS 45.0%
!! ATGGGCTGGAAAGAGAGAAA+AGG + chr8_3:31403989-31404008 Msa1285730:CDS 45.0%
!!! GGAGACCAACCTTATTTTGG+TGG + chr8_3:31404068-31404087 Msa1285730:CDS 45.0%
GGCAAAGAGATGCAAGCAAA+GGG + chr8_3:31404205-31404224 Msa1285730:CDS 45.0%
GTTGGACTGAGAAGACACTT+TGG + chr8_3:31404301-31404320 Msa1285730:exon 45.0%
TTCTTTCCTTTGTTGCCACG+AGG + chr8_3:31404478-31404497 Msa1285730:three_prime_UTR 45.0%
CTCCTCCTCGTAGTTAAGTA+AGG - chr8_3:31404574-31404593 None:intergenic 45.0%
GTTGTCTTTGACAATGGCAG+AGG + chr8_3:31403242-31403261 Msa1285730:exon 45.0%
GTGATGTTGGAGGAAGGATA+TGG + chr8_3:31403970-31403989 Msa1285730:CDS 45.0%
!! ATGGGCTGGAAAGAGAGAAA+AGG + chr8_3:31403989-31404008 Msa1285730:CDS 45.0%
!!! GGAGACCAACCTTATTTTGG+TGG + chr8_3:31404068-31404087 Msa1285730:CDS 45.0%
GGCAAAGAGATGCAAGCAAA+GGG + chr8_3:31404205-31404224 Msa1285730:CDS 45.0%
GTTGGACTGAGAAGACACTT+TGG + chr8_3:31404301-31404320 Msa1285730:exon 45.0%
TTCTTTCCTTTGTTGCCACG+AGG + chr8_3:31404478-31404497 Msa1285730:three_prime_UTR 45.0%
CTCCTCCTCGTAGTTAAGTA+AGG - chr8_3:31404574-31404593 None:intergenic 45.0%
!! AAAATTTAAACTAAATAATT+TGG + chr8_3:31404361-31404380 Msa1285730:three_prime_UTR 5.0%
!! AAAATTTAAACTAAATAATT+TGG + chr8_3:31404361-31404380 Msa1285730:three_prime_UTR 5.0%
!! ATGCAGGTTGGTGATGTTGG+AGG + chr8_3:31403960-31403979 Msa1285730:intron 50.0%
!! AGGTTGGTGATGTTGGAGGA+AGG + chr8_3:31403964-31403983 Msa1285730:intron 50.0%
GGGCAAAGAGATGCAAGCAA+AGG + chr8_3:31404204-31404223 Msa1285730:CDS 50.0%
TTGGAACCTCGTGGCAACAA+AGG - chr8_3:31404487-31404506 None:intergenic 50.0%
!! ATGCAGGTTGGTGATGTTGG+AGG + chr8_3:31403960-31403979 Msa1285730:intron 50.0%
!! AGGTTGGTGATGTTGGAGGA+AGG + chr8_3:31403964-31403983 Msa1285730:intron 50.0%
GGGCAAAGAGATGCAAGCAA+AGG + chr8_3:31404204-31404223 Msa1285730:CDS 50.0%
TTGGAACCTCGTGGCAACAA+AGG - chr8_3:31404487-31404506 None:intergenic 50.0%
GTTGGAGGAAGGATATGGGC+TGG + chr8_3:31403975-31403994 Msa1285730:CDS 55.0%
CGAAGGTGATTGGAACCTCG+TGG - chr8_3:31404496-31404515 None:intergenic 55.0%
GTTGGAGGAAGGATATGGGC+TGG + chr8_3:31403975-31403994 Msa1285730:CDS 55.0%
CGAAGGTGATTGGAACCTCG+TGG - chr8_3:31404496-31404515 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 31403200 31404654 31403200 ID=Msa1285730;Name=Msa1285730
chr8_3 mRNA 31403200 31404654 31403200 ID=Msa1285730-mRNA-1;Parent=Msa1285730;Name=Msa1285730-mRNA-1;_AED=0.06;_eAED=0.06;_QI=55|1|1|1|1|1|2|344|219
chr8_3 exon 31403200 31403569 31403200 ID=Msa1285730-mRNA-1:exon:9949;Parent=Msa1285730-mRNA-1
chr8_3 exon 31403966 31404654 31403966 ID=Msa1285730-mRNA-1:exon:9950;Parent=Msa1285730-mRNA-1
chr8_3 five_prime_UTR 31403200 31403254 31403200 ID=Msa1285730-mRNA-1:five_prime_utr;Parent=Msa1285730-mRNA-1
chr8_3 CDS 31403255 31403569 31403255 ID=Msa1285730-mRNA-1:cds;Parent=Msa1285730-mRNA-1
chr8_3 CDS 31403966 31404310 31403966 ID=Msa1285730-mRNA-1:cds;Parent=Msa1285730-mRNA-1
chr8_3 three_prime_UTR 31404311 31404654 31404311 ID=Msa1285730-mRNA-1:three_prime_utr;Parent=Msa1285730-mRNA-1
Gene Sequence

>Msa1285730

ATGGCAGAGGAGTTGATATTACTTGATGAATGGCTTAGTATATTTGGTATGAGAGCAAGGATAGCATTGGCTGAAAAAGGGATAAAGTATGAGTACAAGGAAGAAGATCTTGAAAACAAGAGCCAAATGCTTTTGCAAATGAATCCAATTCACAAGAAGATTCCAGTTCTTATCCATAAGGGAAAACCAATTTGTGAATCCATCATCATTGTTGAGTATATTGATGAGGTTTGGAAGGATAAGGTCTCATTTCTACCCTCAGATCCTTATGAGAAAGCTCAAGCAAGATTTTGGGCTGATTTTGTCAACAAGAAGGTTGGTGATGTTGGAGGAAGGATATGGGCTGGAAAGAGAGAAAAGGTTGAATTGGTAAAGAAGGAGTTGATTGAAGGCTTAAAGGAATTAGAGAATGTGCTTGGAGACCAACCTTATTTTGGTGGTGACACATTTGGGTTTGTTGACATTGCTTTGATCCCTTTTCATAGTTGGTTTTATACTTATGAGAAATTATGCAACTTCAAAGTTGAGGAAGAGTGTGAAAAACTGATTGTTTGGGCAAAGAGATGCAAGCAAAGGGAGAGTGTTTCCAAATCTGTTGCTGATGAGAAAGAAGTGTATGAATTTGTTGTTAACTATAGGAAGAGGTTAGAGTTGGACTGA
Protein sequence

>Msa1285730

MAEELILLDEWLSIFGMRARIALAEKGIKYEYKEEDLENKSQMLLQMNPIHKKIPVLIHKGKPICESIIIVEYIDEVWKDKVSFLPSDPYEKAQARFWADFVNKKVGDVGGRIWAGKREKVELVKKELIEGLKELENVLGDQPYFGGDTFGFVDIALIPFHSWFYTYEKLCNFKVEEECEKLIVWAKRCKQRESVSKSVADEKEVYEFVVNYRKRLELD*