AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1382130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1382130 MtrunA17_Chr2g0285821 92.537 134 5 3 1 130 1 133 5.87e-82 236
Msa1382130 MtrunA17_Chr8g0393051 72.180 133 16 6 1 129 1 116 5.52e-55 167
Msa1382130 MtrunA17_Chr8g0376421 54.962 131 47 4 2 130 4 124 1.15e-30 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1382130 AT1G30135.1 44.275 131 67 4 1 130 3 128 7.96e-27 97.4
Msa1382130 AT2G34600.1 41.481 135 67 7 1 131 20 146 2.64e-17 73.6

Find 41 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTTACATGGGGTGATTT+TGG 0.252416 tig0020490:+67589 None:intergenic
ATTATGGTTTCAAATATTAA+AGG 0.287910 tig0020490:-64599 Msa1382130:three_prime_UTR
AAAACTGATTCAAATTAAAT+AGG 0.299548 tig0020490:+64707 None:intergenic
TGCACTATTGTTGGTGTTGA+TGG 0.316876 tig0020490:+64880 None:intergenic
GATGATATGGAGATGCTTCT+TGG 0.331037 tig0020490:+64770 None:intergenic
TACTGATGTCACAGAGCTTC+AGG 0.335972 tig0020490:-67369 Msa1382130:intron
GGGTAGGAGGTATATATAAA+TGG 0.338008 tig0020490:+67764 None:intergenic
TGCCAGCATTAAGATGGATT+TGG 0.344981 tig0020490:+64951 None:intergenic
AATTGTTGTATGGAGGAAAA+AGG 0.349461 tig0020490:+67622 None:intergenic
TGTAAATTTCGGCGTGGAAA+CGG 0.378677 tig0020490:+67793 None:intergenic
AATGGTGTAAATGTAAATTT+CGG 0.383436 tig0020490:+67782 None:intergenic
CATCTTGCCATCGTAGAAAA+TGG 0.392349 tig0020490:+67397 None:intergenic
TGAATCTAAATTACAATGTC+AGG 0.402678 tig0020490:+64567 None:intergenic
GTTGATGGTTCTGACCCTAT+TGG 0.411337 tig0020490:+64895 None:intergenic
TCTATTTGCCAGCATTAAGA+TGG 0.436860 tig0020490:+64945 None:intergenic
TTCATGGAAAGACCTGGTCC+AGG 0.470860 tig0020490:+64835 None:intergenic
GAGAGAAGAGAGTGGGTAGG+AGG 0.472903 tig0020490:+67751 None:intergenic
TCTACAAAAGAGAAAGAATA+GGG 0.475189 tig0020490:-64794 Msa1382130:CDS
TTCTACAAAAGAGAAAGAAT+AGG 0.501770 tig0020490:-64795 Msa1382130:CDS
TGAGGAGATTGCACTATTGT+TGG 0.504260 tig0020490:+64871 None:intergenic
TGATTGCTAGAATTGTTGTA+TGG 0.511394 tig0020490:+67612 None:intergenic
GGCCAAATCCATCTTAATGC+TGG 0.519970 tig0020490:-64953 Msa1382130:CDS
CCATGAAAAGATCTTTGCAA+AGG 0.529406 tig0020490:-64819 Msa1382130:CDS
CCTCATCAATTATATAGCCC+TGG 0.530434 tig0020490:-64853 Msa1382130:CDS
TCAATAAAGGGTAGAAGAAG+AGG 0.532802 tig0020490:-67465 Msa1382130:intron
AGGTATAGAGAGAAGAGAGT+GGG 0.543087 tig0020490:+67744 None:intergenic
GATGAGGAGGAATTGCAACT+TGG 0.553645 tig0020490:-67652 Msa1382130:CDS
CGGCGTGGAAACGGCTGAGA+GGG 0.553798 tig0020490:+67802 None:intergenic
AAGGTATAGAGAGAAGAGAG+TGG 0.558139 tig0020490:+67743 None:intergenic
AAAATACAGAAAAGATGAGG+AGG 0.570322 tig0020490:-67665 Msa1382130:exon
CAATTATATAGCCCTGGACC+AGG 0.570726 tig0020490:-64847 Msa1382130:CDS
AATGCTGGCAAATAGAATGA+AGG 0.571363 tig0020490:-64938 Msa1382130:CDS
GAAAATGTAAGCATAAGAGA+AGG 0.579607 tig0020490:+64646 None:intergenic
ATAGAGAGAAGAGAGTGGGT+AGG 0.591817 tig0020490:+67748 None:intergenic
TCATGGAAAGACCTGGTCCA+GGG 0.597585 tig0020490:+64836 None:intergenic
TAGAAAATACAGAAAAGATG+AGG 0.625542 tig0020490:-67668 Msa1382130:exon
TTGCTAGAATTGTTGTATGG+AGG 0.628547 tig0020490:+67615 None:intergenic
CCAGGGCTATATAATTGATG+AGG 0.642818 tig0020490:+64853 None:intergenic
TCGGCGTGGAAACGGCTGAG+AGG 0.667558 tig0020490:+67801 None:intergenic
CCATCGTAGAAAATGGTCAG+TGG 0.671131 tig0020490:+67404 None:intergenic
TGTAAATGTAAATTTCGGCG+TGG 0.681497 tig0020490:+67787 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTAACATTGATTTAA+CGG - tig0020490:65586-65605 Msa1382130:intron 10.0%
!!! TATTTTGAAATAATTTGAAA+TGG + tig0020490:65815-65834 None:intergenic 10.0%
!! TAAATTTATATCAAGTATTT+AGG + tig0020490:66293-66312 None:intergenic 10.0%
!!! ATAAATAAATATAGATTTTG+AGG - tig0020490:66331-66350 Msa1382130:intron 10.0%
!! AGTATAAAATAGTATAAATT+TGG + tig0020490:66715-66734 None:intergenic 10.0%
!!! TTTCATTTTTTTTGTTTTTT+TGG - tig0020490:67672-67691 Msa1382130:exon 10.0%
!!! TTTGTTAATTTGTTCAATAA+AGG - tig0020490:64821-64840 Msa1382130:CDS 15.0%
!!! TTGTTAATTTGTTCAATAAA+GGG - tig0020490:64822-64841 Msa1382130:CDS 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + tig0020490:65179-65198 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + tig0020490:65180-65199 None:intergenic 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG + tig0020490:65244-65263 None:intergenic 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG + tig0020490:65245-65264 None:intergenic 15.0%
!!! ATATAAATTTAGTTTTCATG+AGG - tig0020490:66302-66321 Msa1382130:intron 15.0%
!! AAAATACAAATTCATATCAA+GGG + tig0020490:66621-66640 None:intergenic 15.0%
!! AAAAATACAAATTCATATCA+AGG + tig0020490:66622-66641 None:intergenic 15.0%
!!! ATGAATTTGTATTTTTTTAC+CGG - tig0020490:66626-66645 Msa1382130:intron 15.0%
!! AAAAAATTAACAAATTCATC+CGG + tig0020490:66648-66667 None:intergenic 15.0%
!! AAACCTTAAAAAGAAAATTA+AGG - tig0020490:66886-66905 Msa1382130:intron 15.0%
!!! TATCCTTAATTTTCTTTTTA+AGG + tig0020490:66892-66911 None:intergenic 15.0%
!!! TCATTTTTGTTAAAAAAGTT+TGG - tig0020490:67038-67057 Msa1382130:intron 15.0%
!!! AAAACTGATTCAAATTAAAT+AGG + tig0020490:67595-67614 None:intergenic 15.0%
!! ATTATGGTTTCAAATATTAA+AGG - tig0020490:67700-67719 Msa1382130:five_prime_UTR 15.0%
!! AATGGTGTAAATGTAAATTT+CGG + tig0020490:64520-64539 None:intergenic 20.0%
!!! AAAACAAATTTTGCTTACAT+GGG + tig0020490:64723-64742 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+GGG + tig0020490:65220-65239 None:intergenic 20.0%
!!! AGAAAAGTGTATAAATAAAG+GGG + tig0020490:65243-65262 None:intergenic 20.0%
!!! TAATTGTTTTGGTTTTAGTT+TGG - tig0020490:65419-65438 Msa1382130:intron 20.0%
!!! CATAAAAAGTCACAATTTTA+AGG + tig0020490:65657-65676 None:intergenic 20.0%
!! AAATGGATATTATTGTTGAA+AGG + tig0020490:65798-65817 None:intergenic 20.0%
!!! TTTTGATGGTTAGTTTAAAT+TGG + tig0020490:66229-66248 None:intergenic 20.0%
!!! TGCGGAAAATAATTTTTAAA+AGG - tig0020490:66409-66428 Msa1382130:intron 20.0%
!! AATTAACTGCTAGAAATATT+AGG + tig0020490:66776-66795 None:intergenic 20.0%
!! CTATAAAGTTTGAAAATTCT+TGG + tig0020490:67152-67171 None:intergenic 20.0%
!!! TGTTTTTTTGGAAAGAATTA+TGG - tig0020490:67684-67703 Msa1382130:five_prime_UTR 20.0%
! TAGAAAATACAGAAAAGATG+AGG - tig0020490:64631-64650 Msa1382130:three_prime_UTR 25.0%
!! AAACAAATTTTGCTTACATG+GGG + tig0020490:64722-64741 None:intergenic 25.0%
!! GAAAACAAATTTTGCTTACA+TGG + tig0020490:64724-64743 None:intergenic 25.0%
!!! TATGTGATTGTTGTTTTCTT+TGG - tig0020490:64782-64801 Msa1382130:CDS 25.0%
!!! ATGTGATTGTTGTTTTCTTT+GGG - tig0020490:64783-64802 Msa1382130:CDS 25.0%
! ATCATATCATCTTCAATCAT+AGG + tig0020490:65009-65028 None:intergenic 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + tig0020490:65111-65130 None:intergenic 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG + tig0020490:65132-65151 None:intergenic 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG + tig0020490:65133-65152 None:intergenic 25.0%
! GAAAGTGGAATATATTGTAT+TGG + tig0020490:65221-65240 None:intergenic 25.0%
! CTGGTTATCATATTCAATTA+TGG - tig0020490:65367-65386 Msa1382130:intron 25.0%
! TTCAATTATGGATCATAAGA+AGG - tig0020490:65379-65398 Msa1382130:intron 25.0%
! CCAAAACAATTAATTAAGCT+GGG + tig0020490:65411-65430 None:intergenic 25.0%
! ACCAAAACAATTAATTAAGC+TGG + tig0020490:65412-65431 None:intergenic 25.0%
!! AAAGTGATAATGTTTGCATT+TGG + tig0020490:65485-65504 None:intergenic 25.0%
!!! CATTGATTTAACGGATTTTT+CGG - tig0020490:65595-65614 Msa1382130:intron 25.0%
! TTGTTGAAAGGTTAAATCAA+GGG + tig0020490:65786-65805 None:intergenic 25.0%
! ATTGTTGAAAGGTTAAATCA+AGG + tig0020490:65787-65806 None:intergenic 25.0%
!!! AGTAAATGACTTTTTTTTGG+TGG + tig0020490:65878-65897 None:intergenic 25.0%
!!! AGGAGTAAATGACTTTTTTT+TGG + tig0020490:65881-65900 None:intergenic 25.0%
! CTATGAGACTCATAATATAT+GGG + tig0020490:65908-65927 None:intergenic 25.0%
! TCTATGAGACTCATAATATA+TGG + tig0020490:65909-65928 None:intergenic 25.0%
!!! ACTGGTCTTATTTAGAAATT+TGG + tig0020490:66075-66094 None:intergenic 25.0%
!!! TTAGTTTAAATTGGTTTGAG+AGG + tig0020490:66220-66239 None:intergenic 25.0%
!!! AAATTTGGTGTGAATTTCAA+TGG + tig0020490:66700-66719 None:intergenic 25.0%
!! TTTTGTAAAAGCAAGCTAAT+TGG - tig0020490:67119-67138 Msa1382130:intron 25.0%
! CAGTATAAGGTCAATTATTT+CGG - tig0020490:67234-67253 Msa1382130:intron 25.0%
! TCAATTATTTCGGATAAGAA+AGG - tig0020490:67244-67263 Msa1382130:intron 25.0%
! TTCTACAAAAGAGAAAGAAT+AGG - tig0020490:67504-67523 Msa1382130:intron 25.0%
! TCTACAAAAGAGAAAGAATA+GGG - tig0020490:67505-67524 Msa1382130:intron 25.0%
! AGGCTATGCTAAAAAAAATA+AGG + tig0020490:67575-67594 None:intergenic 25.0%
! TGAATCTAAATTACAATGTC+AGG + tig0020490:67735-67754 None:intergenic 25.0%
GATGAAGCAAAAATGTGTTA+AGG + tig0020490:64578-64597 None:intergenic 30.0%
AAAATACAGAAAAGATGAGG+AGG - tig0020490:64634-64653 Msa1382130:three_prime_UTR 30.0%
AATTGTTGTATGGAGGAAAA+AGG + tig0020490:64680-64699 None:intergenic 30.0%
TGATTGCTAGAATTGTTGTA+TGG + tig0020490:64690-64709 None:intergenic 30.0%
!! ATGTTAGCATTTGATTTGAG+TGG - tig0020490:64983-65002 Msa1382130:intron 30.0%
AGAAAATGGATCAGTTATTC+CGG + tig0020490:65097-65116 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG + tig0020490:65236-65255 None:intergenic 30.0%
TCTGTGTGAAAAACTAAAGT+AGG - tig0020490:65266-65285 Msa1382130:intron 30.0%
CTAAAGTAGGTCAATTATAC+CGG - tig0020490:65279-65298 Msa1382130:intron 30.0%
TAAAGTAGGTCAATTATACC+GGG - tig0020490:65280-65299 Msa1382130:intron 30.0%
! CCCAGCTTAATTAATTGTTT+TGG - tig0020490:65408-65427 Msa1382130:intron 30.0%
!!! TTTAACGGATTTTTCGGAAA+TGG - tig0020490:65601-65620 Msa1382130:intron 30.0%
!!! ATGACTTTTTTTTGGTGGAA+AGG + tig0020490:65873-65892 None:intergenic 30.0%
ACTCATAATATATGGGCAAA+AGG + tig0020490:65901-65920 None:intergenic 30.0%
AAATACACATATTGAGAGAG+AGG - tig0020490:66004-66023 Msa1382130:intron 30.0%
!! TGACGTTGTTTGTGTTATTT+CGG + tig0020490:66102-66121 None:intergenic 30.0%
ACACAAACAACGTCATATTT+AGG - tig0020490:66107-66126 Msa1382130:intron 30.0%
!!! TTTGATTTTGGTGTTGGTTT+TGG + tig0020490:66252-66271 None:intergenic 30.0%
!! TTTGACTTTGATTTTGGTGT+TGG + tig0020490:66258-66277 None:intergenic 30.0%
CTTGTTAATCAGATTATCTG+CGG - tig0020490:66391-66410 Msa1382130:intron 30.0%
TGAATATAGTTCAATGGGTA+GGG + tig0020490:66548-66567 None:intergenic 30.0%
TACCCATTGAACTATATTCA+CGG - tig0020490:66548-66567 Msa1382130:intron 30.0%
TTCATGTGTTATGCAGTATA+AGG - tig0020490:67221-67240 Msa1382130:intron 30.0%
! TGATTTTGCTAACGATGATA+TGG + tig0020490:67545-67564 None:intergenic 30.0%
!!! CTTCTTTTTTAAACCTTGCA+AGG - tig0020490:67618-67637 Msa1382130:CDS 30.0%
GAGAAAAAAAAAACCTTGCA+AGG + tig0020490:67634-67653 None:intergenic 30.0%
GAAAATGTAAGCATAAGAGA+AGG + tig0020490:67656-67675 None:intergenic 30.0%
TGTAAATGTAAATTTCGGCG+TGG + tig0020490:64515-64534 None:intergenic 35.0%
! GGGTAGGAGGTATATATAAA+TGG + tig0020490:64538-64557 None:intergenic 35.0%
TTGCTAGAATTGTTGTATGG+AGG + tig0020490:64687-64706 None:intergenic 35.0%
TCAATAAAGGGTAGAAGAAG+AGG - tig0020490:64834-64853 Msa1382130:CDS 35.0%
GAAAATGGATCAGTTATTCC+GGG + tig0020490:65096-65115 None:intergenic 35.0%
! AGTTTGAGTTACTCATTCTG+TGG + tig0020490:65349-65368 None:intergenic 35.0%
ACAGAATGAGTAACTCAAAC+TGG - tig0020490:65348-65367 Msa1382130:intron 35.0%
!! TTGTGTTATTTCGGTCTAAC+TGG + tig0020490:66093-66112 None:intergenic 35.0%
CAAACAACGTCATATTTAGG+CGG - tig0020490:66110-66129 Msa1382130:intron 35.0%
TCAAGTATTTAGGCTGTTGT+GGG + tig0020490:66283-66302 None:intergenic 35.0%
ATCAAGTATTTAGGCTGTTG+TGG + tig0020490:66284-66303 None:intergenic 35.0%
GTGAATATAGTTCAATGGGT+AGG + tig0020490:66549-66568 None:intergenic 35.0%
CTCCGTGAATATAGTTCAAT+GGG + tig0020490:66553-66572 None:intergenic 35.0%
TCTCCGTGAATATAGTTCAA+TGG + tig0020490:66554-66573 None:intergenic 35.0%
TTAAAAAAGTTTGGCGTGAG+TGG - tig0020490:67047-67066 Msa1382130:intron 35.0%
TCTATTTGCCAGCATTAAGA+TGG + tig0020490:67357-67376 None:intergenic 35.0%
AATGCTGGCAAATAGAATGA+AGG - tig0020490:67361-67380 Msa1382130:intron 35.0%
AAAAAGTGACGACACCAATA+GGG - tig0020490:67390-67409 Msa1382130:CDS 35.0%
! CCTTTGCAAAGATCTTTTCA+TGG + tig0020490:67483-67502 None:intergenic 35.0%
CCATGAAAAGATCTTTGCAA+AGG - tig0020490:67480-67499 Msa1382130:intron 35.0%
TGTAAATTTCGGCGTGGAAA+CGG + tig0020490:64509-64528 None:intergenic 40.0%
AGGTATAGAGAGAAGAGAGT+GGG + tig0020490:64558-64577 None:intergenic 40.0%
AAGGTATAGAGAGAAGAGAG+TGG + tig0020490:64559-64578 None:intergenic 40.0%
! TTGCTTACATGGGGTGATTT+TGG + tig0020490:64713-64732 None:intergenic 40.0%
CATCTTGCCATCGTAGAAAA+TGG + tig0020490:64905-64924 None:intergenic 40.0%
GTCGCATACATGTCTTATCA+TGG + tig0020490:65035-65054 None:intergenic 40.0%
TGAGTTACTCATTCTGTGGT+AGG + tig0020490:65345-65364 None:intergenic 40.0%
!! TAAGGTCTTAAAGAGTGCTC+CGG + tig0020490:65639-65658 None:intergenic 40.0%
!!! GGTGTTGGTTTTGGTTTTGA+TGG + tig0020490:66243-66262 None:intergenic 40.0%
!! TGGGGGTTTGACTTTGATTT+TGG + tig0020490:66264-66283 None:intergenic 40.0%
AAGTATTTAGGCTGTTGTGG+GGG + tig0020490:66281-66300 None:intergenic 40.0%
CAAGTATTTAGGCTGTTGTG+GGG + tig0020490:66282-66301 None:intergenic 40.0%
TCGAGTTTGAATCTCGGATT+CGG + tig0020490:66506-66525 None:intergenic 40.0%
CAAGAGTCGAGTTTGAATCT+CGG + tig0020490:66512-66531 None:intergenic 40.0%
GAACTATATTCACGGAGAGT+CGG - tig0020490:66556-66575 Msa1382130:intron 40.0%
CTTAGCTTAAGTGCTATGCA+CGG + tig0020490:66811-66830 None:intergenic 40.0%
AGGTTTCACTACCAACTTGT+CGG + tig0020490:66872-66891 None:intergenic 40.0%
TCCACGAAAGAGATAGTAAG+TGG - tig0020490:66915-66934 Msa1382130:intron 40.0%
ACCACTTACTATCTCTTTCG+TGG + tig0020490:66919-66938 None:intergenic 40.0%
CGAAAGAGATAGTAAGTGGT+AGG - tig0020490:66919-66938 Msa1382130:intron 40.0%
AGTAAGTGGTAGGTTCCTAT+AGG - tig0020490:66929-66948 Msa1382130:intron 40.0%
! TGCCAGCATTAAGATGGATT+TGG + tig0020490:67351-67370 None:intergenic 40.0%
GAAAAAGTGACGACACCAAT+AGG - tig0020490:67389-67408 Msa1382130:CDS 40.0%
!! TGCACTATTGTTGGTGTTGA+TGG + tig0020490:67422-67441 None:intergenic 40.0%
TGAGGAGATTGCACTATTGT+TGG + tig0020490:67431-67450 None:intergenic 40.0%
CCAGGGCTATATAATTGATG+AGG + tig0020490:67449-67468 None:intergenic 40.0%
CCTCATCAATTATATAGCCC+TGG - tig0020490:67446-67465 Msa1382130:CDS 40.0%
! GATCTTTTCATGGAAAGACC+TGG + tig0020490:67473-67492 None:intergenic 40.0%
GATGATATGGAGATGCTTCT+TGG + tig0020490:67532-67551 None:intergenic 40.0%
ATAGAGAGAAGAGAGTGGGT+AGG + tig0020490:64554-64573 None:intergenic 45.0%
GATGAGGAGGAATTGCAACT+TGG - tig0020490:64647-64666 Msa1382130:three_prime_UTR 45.0%
!!! GGCTGTTGCTGATTTTGCAT+TGG + tig0020490:64877-64896 None:intergenic 45.0%
CCATCGTAGAAAATGGTCAG+TGG + tig0020490:64898-64917 None:intergenic 45.0%
! CCACTGACCATTTTCTACGA+TGG - tig0020490:64895-64914 Msa1382130:CDS 45.0%
TACTGATGTCACAGAGCTTC+AGG - tig0020490:64930-64949 Msa1382130:CDS 45.0%
ATGGATCAGTTATTCCGGGA+CGG + tig0020490:65092-65111 None:intergenic 45.0%
GTAGGTCAATTATACCGGGA+CGG - tig0020490:65284-65303 Msa1382130:intron 45.0%
ACTATATACTACCTCCGTCC+CGG + tig0020490:65301-65320 None:intergenic 45.0%
!! AAGGTCTTAAAGAGTGCTCC+GGG + tig0020490:65638-65657 None:intergenic 45.0%
!! GAGATTCCTTAAGAGTGCTC+CGG - tig0020490:65745-65764 Msa1382130:intron 45.0%
!! AGGGTCTTGTTAACGAGTTC+CGG + tig0020490:65767-65786 None:intergenic 45.0%
!! TGGCGTTGTTGTTGGTTTGT+TGG + tig0020490:66182-66201 None:intergenic 45.0%
CATTTGAGTGGCGTTGTTGT+TGG + tig0020490:66190-66209 None:intergenic 45.0%
ACAACAACGCCACTCAAATG+AGG - tig0020490:66190-66209 Msa1382130:intron 45.0%
AGAGGTAGTCCTCATTTGAG+TGG + tig0020490:66202-66221 None:intergenic 45.0%
CGGATTCGGATTCACTTAAG+TGG + tig0020490:66492-66511 None:intergenic 45.0%
AATAGTCGTTGCCGACAAGT+TGG - tig0020490:66858-66877 Msa1382130:intron 45.0%
AAGTGGTAGGTTCCTATAGG+TGG - tig0020490:66932-66951 Msa1382130:intron 45.0%
CATGCTCATACACCACCTAT+AGG + tig0020490:66947-66966 None:intergenic 45.0%
TGTTAACGTGAATGTCGTGC+AGG - tig0020490:67325-67344 Msa1382130:intron 45.0%
GGCCAAATCCATCTTAATGC+TGG - tig0020490:67346-67365 Msa1382130:intron 45.0%
!! GTTGATGGTTCTGACCCTAT+TGG + tig0020490:67407-67426 None:intergenic 45.0%
CAATTATATAGCCCTGGACC+AGG - tig0020490:67452-67471 Msa1382130:CDS 45.0%
!! AAATTAAATTTAAAAATGAA+AGG + tig0020490:64760-64779 None:intergenic 5.0%
AGTTATTACTCCCTCCGTCC+CGG - tig0020490:65075-65094 Msa1382130:intron 50.0%
ATCAGTTATTCCGGGACGGA+GGG + tig0020490:65088-65107 None:intergenic 50.0%
!! AGGTCTTAAAGAGTGCTCCG+GGG + tig0020490:65637-65656 None:intergenic 50.0%
TCATGGAAAGACCTGGTCCA+GGG + tig0020490:67466-67485 None:intergenic 50.0%
TTCATGGAAAGACCTGGTCC+AGG + tig0020490:67467-67486 None:intergenic 50.0%
GAGAGAAGAGAGTGGGTAGG+AGG + tig0020490:64551-64570 None:intergenic 55.0%
GATCAGTTATTCCGGGACGG+AGG + tig0020490:65089-65108 None:intergenic 55.0%
GGTCAATTATACCGGGACGG+AGG - tig0020490:65287-65306 Msa1382130:intron 55.0%
! GAAATGGTAACGAGTGCCCC+CGG - tig0020490:65617-65636 Msa1382130:intron 55.0%
!! GGTCTTAAAGAGTGCTCCGG+GGG + tig0020490:65636-65655 None:intergenic 55.0%
! CGAGTTCCGGAGCACTCTTA+AGG + tig0020490:65754-65773 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0020490 gene 64499 67822 64499 ID=Msa1382130;Name=Msa1382130
tig0020490 mRNA 64499 67822 64499 ID=Msa1382130-mRNA-1;Parent=Msa1382130;Name=Msa1382130-mRNA-1;_AED=0.05;_eAED=0.05;_QI=149|1|1|1|1|1|3|266|131
tig0020490 exon 64499 64974 64499 ID=Msa1382130-mRNA-1:exon:7943;Parent=Msa1382130-mRNA-1
tig0020490 exon 67370 67478 67370 ID=Msa1382130-mRNA-1:exon:7942;Parent=Msa1382130-mRNA-1
tig0020490 exon 67597 67822 67597 ID=Msa1382130-mRNA-1:exon:7941;Parent=Msa1382130-mRNA-1
tig0020490 five_prime_UTR 67674 67822 67674 ID=Msa1382130-mRNA-1:five_prime_utr;Parent=Msa1382130-mRNA-1
tig0020490 CDS 67597 67673 67597 ID=Msa1382130-mRNA-1:cds;Parent=Msa1382130-mRNA-1
tig0020490 CDS 67370 67478 67370 ID=Msa1382130-mRNA-1:cds;Parent=Msa1382130-mRNA-1
tig0020490 CDS 64765 64974 64765 ID=Msa1382130-mRNA-1:cds;Parent=Msa1382130-mRNA-1
tig0020490 three_prime_UTR 64499 64764 64499 ID=Msa1382130-mRNA-1:three_prime_utr;Parent=Msa1382130-mRNA-1
Gene Sequence

>Msa1382130

ATGAGGAGGAATTGCAACTTGGAACTATGCCTTTTTCCTCCATACAACAATTCTAGCAATCACCAAAATCACCCCATGGTAGAAGAAGAGGATAGTAATGAGAGCACTCCAATGCAAAATCAGCAACAGCCACTGACCATTTTCTACGATGGCAAGATGTGTGTTACTGATGTCACAGAGCTTCAGGCCAAATCCATCTTAATGCTGGCAAATAGAATGAAGGTGCTAGAAAAAGTGACGACACCAATAGGGTCAGAACCATCAACACCAACAATAGTGCAATCTCCTCATCAATTATATAGCCCTGGACCAGGTCTTTCCATGAAAAGATCTTTGCAAAGGTTTCTACAAAAGAGAAAGAATAGGGTCCAAGAAGCATCTCCATATCATCGTTAG
Protein sequence

>Msa1382130

MRRNCNLELCLFPPYNNSSNHQNHPMVEEEDSNESTPMQNQQQPLTIFYDGKMCVTDVTELQAKSILMLANRMKVLEKVTTPIGSEPSTPTIVQSPHQLYSPGPGLSMKRSLQRFLQKRKNRVQEASPYHR*