AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1382960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1382960 MtrunA17_Chr3g0146071 98.148 54 1 0 15 68 445 498 4.00e-28 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 83 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAACAAAATGTAATATTA+TGG 0.135823 tig0020545:-1421 None:intergenic
AAGGCATCATCTGCAATATT+AGG 0.220525 tig0020545:+44 Msa1382960:five_prime_UTR
GCTTACTTCTCCATTTGTTT+TGG 0.253315 tig0020545:-194 None:intergenic
GATCAAAGGTTTAGATATAT+TGG 0.260429 tig0020545:-544 None:intergenic
TAAAGGAATTCCGATAGATT+TGG 0.291608 tig0020545:+362 Msa1382960:five_prime_UTR
GGAACAAAATGTAATATTAT+GGG 0.300244 tig0020545:-1420 None:intergenic
CTCTTAGTCTCTACAGTTAC+CGG 0.309294 tig0020545:-432 None:intergenic
AGCCTCTATAGTTGTGTCTT+TGG 0.310801 tig0020545:-754 None:intergenic
CTTCATTCCTATTCCTTGAT+AGG 0.316654 tig0020545:-651 None:intergenic
GTGATATAAAGCCTTAAAAT+AGG 0.325910 tig0020545:-1378 None:intergenic
CTAGTAGTGATAACCCTATT+GGG 0.351967 tig0020545:+456 Msa1382960:five_prime_UTR
ATATCTAAACCTTTGATCAT+TGG 0.355196 tig0020545:+549 Msa1382960:five_prime_UTR
GCTGGGTGTTCCAACATCTA+TGG 0.356749 tig0020545:+479 Msa1382960:five_prime_UTR
AAAGGAATTCCGATAGATTT+GGG 0.377796 tig0020545:+363 Msa1382960:five_prime_UTR
AATTATACAATTTGTAATGC+TGG 0.378211 tig0020545:+943 Msa1382960:CDS
AAAGTAACATAGTGAGTAGA+AGG 0.381395 tig0020545:+114 Msa1382960:five_prime_UTR
AATGGGAAGATTAGAGTTAG+AGG 0.381732 tig0020545:+166 Msa1382960:five_prime_UTR
TTCACATTATCTTCATCAAC+TGG 0.383240 tig0020545:-1200 None:intergenic
AAATGCAACATGCTTAAATA+AGG 0.384967 tig0020545:+393 Msa1382960:five_prime_UTR
TTGGAACACCCAGCCCCAAT+AGG 0.395905 tig0020545:-470 None:intergenic
ACTAGTAGTGATAACCCTAT+TGG 0.407453 tig0020545:+455 Msa1382960:five_prime_UTR
TCTAATCTTCCCATTCATAA+AGG 0.408185 tig0020545:-158 None:intergenic
ATGCCTTCTCGAACTATGAT+TGG 0.408417 tig0020545:-28 None:intergenic
GCACATTATACCTTTATGAA+TGG 0.411365 tig0020545:+148 Msa1382960:five_prime_UTR
TGCATTGTTGTGCAATCCTC+AGG 0.417557 tig0020545:+850 Msa1382960:five_prime_UTR
AGAGAATATTGTGACTGATT+TGG 0.419588 tig0020545:+1229 Msa1382960:CDS
AGGCATCATCTGCAATATTA+GGG 0.433884 tig0020545:+45 Msa1382960:five_prime_UTR
CACATTATACCTTTATGAAT+GGG 0.435349 tig0020545:+149 Msa1382960:five_prime_UTR
TAGTGTGTTGCTGCCTATCA+AGG 0.441713 tig0020545:+638 Msa1382960:five_prime_UTR
TCACTTCAGATCCGTCTCTC+TGG 0.446265 tig0020545:-1252 None:intergenic
AGATATATTGGAACAATTGA+GGG 0.454641 tig0020545:-532 None:intergenic
TAGATATATTGGAACAATTG+AGG 0.457243 tig0020545:-533 None:intergenic
GTTGCTGCCTATCAAGGAAT+AGG 0.458638 tig0020545:+644 Msa1382960:five_prime_UTR
AGTGATAACCCTATTGGGGC+TGG 0.463025 tig0020545:+461 Msa1382960:five_prime_UTR
AGAGGTAGAGCCAAAACAAA+TGG 0.466353 tig0020545:+184 Msa1382960:five_prime_UTR
CCGCACTCAAACAAACGAAC+TGG 0.471505 tig0020545:-1462 None:intergenic
TTACAAACAAACTCATGGTA+TGG 0.487888 tig0020545:+918 Msa1382960:exon
TGGAACACCCAGCCCCAATA+GGG 0.502134 tig0020545:-469 None:intergenic
AGACACAACTATAGAGGCTC+TGG 0.509549 tig0020545:+758 Msa1382960:five_prime_UTR
TAATGCTGGCTTATCATGCC+AGG 0.517915 tig0020545:+957 Msa1382960:CDS
TGAGTAGAAGGGTTATAAGC+AGG 0.525564 tig0020545:+126 Msa1382960:five_prime_UTR
ATTCCGATAGATTTGGGAGT+GGG 0.527577 tig0020545:+369 Msa1382960:five_prime_UTR
TAGAGAGCACTCAAGGGTCT+CGG 0.527622 tig0020545:+996 Msa1382960:CDS
GTCCAAAGACACAACTATAG+AGG 0.528267 tig0020545:+752 Msa1382960:five_prime_UTR
TTCCCCACTCCCAAATCTAT+CGG 0.531794 tig0020545:-372 None:intergenic
TCAAGGAATAGGAATGAAGA+TGG 0.532880 tig0020545:+655 Msa1382960:five_prime_UTR
GACTGATTTGGCCAGAGAGA+CGG 0.534839 tig0020545:+1241 Msa1382960:CDS
GTGATAACCCTATTGGGGCT+GGG 0.535516 tig0020545:+462 Msa1382960:five_prime_UTR
AAAGAACTAGAGAGCACTCA+AGG 0.537212 tig0020545:+989 Msa1382960:CDS
GTAAGTCTAGGCTGCGGTGT+TGG 0.537341 tig0020545:+245 Msa1382960:five_prime_UTR
TTAATTTACAAACAAACTCA+TGG 0.544439 tig0020545:+913 Msa1382960:five_prime_UTR
AAGCATCTGCAATAACCTCA+TGG 0.548952 tig0020545:+213 Msa1382960:five_prime_UTR
GAACACGAGGCAGACATGAA+TGG 0.556258 tig0020545:+322 Msa1382960:five_prime_UTR
TAACGTCAACCAATGATCAA+AGG 0.559124 tig0020545:-558 None:intergenic
ACAATGCAATTCATTCATAG+AGG 0.560651 tig0020545:-835 None:intergenic
ATAATACAAAGTGTGATACA+TGG 0.567953 tig0020545:-706 None:intergenic
GCAATAACCTCATGGGCAGA+AGG 0.568035 tig0020545:+221 Msa1382960:five_prime_UTR
TGAATGGACTCAGAGCGAAC+AGG 0.569377 tig0020545:+338 Msa1382960:five_prime_UTR
ACTCAGAGCGAACAGGCTAA+AGG 0.571527 tig0020545:+345 Msa1382960:five_prime_UTR
CCAGTTCGTTTGTTTGAGTG+CGG 0.571750 tig0020545:+1462 Msa1382960:three_prime_UTR
ATTGAGATGTAGCTGTTGCT+TGG 0.573298 tig0020545:+1328 Msa1382960:three_prime_UTR
TGGGCAGAAGGAGTAAGTCT+AGG 0.574686 tig0020545:+233 Msa1382960:five_prime_UTR
AGCATCTGCAATAACCTCAT+GGG 0.586558 tig0020545:+214 Msa1382960:five_prime_UTR
AGGAATAGGAATGAAGATGG+AGG 0.586780 tig0020545:+658 Msa1382960:five_prime_UTR
TAATACAAAGTGTGATACAT+GGG 0.587197 tig0020545:-705 None:intergenic
TTTCCAATCATAGTTCGAGA+AGG 0.589465 tig0020545:+25 Msa1382960:five_prime_UTR
TTCCGATAGATTTGGGAGTG+GGG 0.596683 tig0020545:+370 Msa1382960:five_prime_UTR
GCTTTATATCACATGACAAG+TGG 0.608820 tig0020545:+1389 Msa1382960:three_prime_UTR
AATTCCGATAGATTTGGGAG+TGG 0.619378 tig0020545:+368 Msa1382960:five_prime_UTR
AAGTAACATAGTGAGTAGAA+GGG 0.620175 tig0020545:+115 Msa1382960:five_prime_UTR
ATGGAGGAAAATCTCAACAA+AGG 0.626766 tig0020545:+674 Msa1382960:five_prime_UTR
ACTTACTCCTTCTGCCCATG+AGG 0.632862 tig0020545:-228 None:intergenic
TAGTAGTGATAACCCTATTG+GGG 0.633592 tig0020545:+457 Msa1382960:five_prime_UTR
TGAAAGCAAAATGATGACAA+AGG 0.643687 tig0020545:-1353 None:intergenic
TCTTAGTCTCTACAGTTACC+GGG 0.650768 tig0020545:-431 None:intergenic
GAAGGAGTAAGTCTAGGCTG+CGG 0.659056 tig0020545:+239 Msa1382960:five_prime_UTR
TCACGATGATAGACTTGCGA+AGG 0.662542 tig0020545:+280 Msa1382960:five_prime_UTR
AAGAACTAGAGAGCACTCAA+GGG 0.664552 tig0020545:+990 Msa1382960:CDS
AGGTAGCATAAACTTACACC+CGG 0.672371 tig0020545:+413 Msa1382960:five_prime_UTR
TAAAATATATTAGCTGCCTG+AGG 0.675394 tig0020545:-866 None:intergenic
TGCTGGCTTATCATGCCAGG+AGG 0.701100 tig0020545:+960 Msa1382960:CDS
ATAGAATTATCTTGAACACG+AGG 0.723820 tig0020545:+309 Msa1382960:five_prime_UTR
TGTAGTTGAAACAAGCGTGA+AGG 0.725827 tig0020545:+1026 Msa1382960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATAAATAATTTTCAACAG+AGG - tig0020545:894-913 None:intergenic 15.0%
!!! ATATAAATAATTTTCAACAG+AGG - tig0020545:894-913 None:intergenic 15.0%
!! TTAATTTACAAACAAACTCA+TGG + tig0020545:913-932 Msa1382960:five_prime_UTR 20.0%
!! AATTATACAATTTGTAATGC+TGG + tig0020545:943-962 Msa1382960:CDS 20.0%
!!! TGCATTTTATTGAAAATATG+TGG + tig0020545:1116-1135 Msa1382960:intron 20.0%
!! AAACTTCTACTTATTGAATT+TGG + tig0020545:1147-1166 Msa1382960:intron 20.0%
!! GGAACAAAATGTAATATTAT+GGG - tig0020545:1423-1442 None:intergenic 20.0%
!! TGGAACAAAATGTAATATTA+TGG - tig0020545:1424-1443 None:intergenic 20.0%
!! TTAATTTACAAACAAACTCA+TGG + tig0020545:913-932 Msa1382960:five_prime_UTR 20.0%
!! AATTATACAATTTGTAATGC+TGG + tig0020545:943-962 Msa1382960:CDS 20.0%
!!! TGCATTTTATTGAAAATATG+TGG + tig0020545:1116-1135 Msa1382960:intron 20.0%
!! AAACTTCTACTTATTGAATT+TGG + tig0020545:1147-1166 Msa1382960:intron 20.0%
!! GGAACAAAATGTAATATTAT+GGG - tig0020545:1423-1442 None:intergenic 20.0%
!! TGGAACAAAATGTAATATTA+TGG - tig0020545:1424-1443 None:intergenic 20.0%
!! TTTGTCTTCATGATCTTTTA+TGG - tig0020545:75-94 None:intergenic 25.0%
! CACATTATACCTTTATGAAT+GGG + tig0020545:149-168 Msa1382960:five_prime_UTR 25.0%
! AAATGCAACATGCTTAAATA+AGG + tig0020545:393-412 Msa1382960:five_prime_UTR 25.0%
!!! TCTTTTAATTCCATAGATGT+TGG - tig0020545:492-511 None:intergenic 25.0%
! AGATATATTGGAACAATTGA+GGG - tig0020545:535-554 None:intergenic 25.0%
! TAGATATATTGGAACAATTG+AGG - tig0020545:536-555 None:intergenic 25.0%
! GATCAAAGGTTTAGATATAT+TGG - tig0020545:547-566 None:intergenic 25.0%
! ATATCTAAACCTTTGATCAT+TGG + tig0020545:549-568 Msa1382960:five_prime_UTR 25.0%
! TAATACAAAGTGTGATACAT+GGG - tig0020545:708-727 None:intergenic 25.0%
! ATAATACAAAGTGTGATACA+TGG - tig0020545:709-728 None:intergenic 25.0%
!! AGCTACATCTCAATTATTTT+AGG - tig0020545:1322-1341 None:intergenic 25.0%
! GTGATATAAAGCCTTAAAAT+AGG - tig0020545:1381-1400 None:intergenic 25.0%
!! TTTGTCTTCATGATCTTTTA+TGG - tig0020545:75-94 None:intergenic 25.0%
! CACATTATACCTTTATGAAT+GGG + tig0020545:149-168 Msa1382960:five_prime_UTR 25.0%
! AAATGCAACATGCTTAAATA+AGG + tig0020545:393-412 Msa1382960:five_prime_UTR 25.0%
!!! TCTTTTAATTCCATAGATGT+TGG - tig0020545:492-511 None:intergenic 25.0%
! AGATATATTGGAACAATTGA+GGG - tig0020545:535-554 None:intergenic 25.0%
! TAGATATATTGGAACAATTG+AGG - tig0020545:536-555 None:intergenic 25.0%
! GATCAAAGGTTTAGATATAT+TGG - tig0020545:547-566 None:intergenic 25.0%
! ATATCTAAACCTTTGATCAT+TGG + tig0020545:549-568 Msa1382960:five_prime_UTR 25.0%
! TAATACAAAGTGTGATACAT+GGG - tig0020545:708-727 None:intergenic 25.0%
! ATAATACAAAGTGTGATACA+TGG - tig0020545:709-728 None:intergenic 25.0%
!! AGCTACATCTCAATTATTTT+AGG - tig0020545:1322-1341 None:intergenic 25.0%
! GTGATATAAAGCCTTAAAAT+AGG - tig0020545:1381-1400 None:intergenic 25.0%
AAAGTAACATAGTGAGTAGA+AGG + tig0020545:114-133 Msa1382960:five_prime_UTR 30.0%
AAGTAACATAGTGAGTAGAA+GGG + tig0020545:115-134 Msa1382960:five_prime_UTR 30.0%
GCACATTATACCTTTATGAA+TGG + tig0020545:148-167 Msa1382960:five_prime_UTR 30.0%
TCTAATCTTCCCATTCATAA+AGG - tig0020545:161-180 None:intergenic 30.0%
ATAGAATTATCTTGAACACG+AGG + tig0020545:309-328 Msa1382960:five_prime_UTR 30.0%
TAAAGGAATTCCGATAGATT+TGG + tig0020545:362-381 Msa1382960:five_prime_UTR 30.0%
AAAGGAATTCCGATAGATTT+GGG + tig0020545:363-382 Msa1382960:five_prime_UTR 30.0%
ACAATGCAATTCATTCATAG+AGG - tig0020545:838-857 None:intergenic 30.0%
TAAAATATATTAGCTGCCTG+AGG - tig0020545:869-888 None:intergenic 30.0%
TTACAAACAAACTCATGGTA+TGG + tig0020545:918-937 Msa1382960:exon 30.0%
!! TTATTGAATTTGGTTCTTGC+AGG + tig0020545:1157-1176 Msa1382960:intron 30.0%
TTCACATTATCTTCATCAAC+TGG - tig0020545:1203-1222 None:intergenic 30.0%
AGAGAATATTGTGACTGATT+TGG + tig0020545:1229-1248 Msa1382960:CDS 30.0%
TGAAAGCAAAATGATGACAA+AGG - tig0020545:1356-1375 None:intergenic 30.0%
! TTGCTTTCATGCCTATTTTA+AGG + tig0020545:1367-1386 Msa1382960:three_prime_UTR 30.0%
!!! GGAGTACTATTTTGCTATTT+TGG - tig0020545:1444-1463 None:intergenic 30.0%
AAAGTAACATAGTGAGTAGA+AGG + tig0020545:114-133 Msa1382960:five_prime_UTR 30.0%
AAGTAACATAGTGAGTAGAA+GGG + tig0020545:115-134 Msa1382960:five_prime_UTR 30.0%
GCACATTATACCTTTATGAA+TGG + tig0020545:148-167 Msa1382960:five_prime_UTR 30.0%
TCTAATCTTCCCATTCATAA+AGG - tig0020545:161-180 None:intergenic 30.0%
ATAGAATTATCTTGAACACG+AGG + tig0020545:309-328 Msa1382960:five_prime_UTR 30.0%
TAAAGGAATTCCGATAGATT+TGG + tig0020545:362-381 Msa1382960:five_prime_UTR 30.0%
AAAGGAATTCCGATAGATTT+GGG + tig0020545:363-382 Msa1382960:five_prime_UTR 30.0%
ACAATGCAATTCATTCATAG+AGG - tig0020545:838-857 None:intergenic 30.0%
TAAAATATATTAGCTGCCTG+AGG - tig0020545:869-888 None:intergenic 30.0%
TTACAAACAAACTCATGGTA+TGG + tig0020545:918-937 Msa1382960:exon 30.0%
!! TTATTGAATTTGGTTCTTGC+AGG + tig0020545:1157-1176 Msa1382960:intron 30.0%
TTCACATTATCTTCATCAAC+TGG - tig0020545:1203-1222 None:intergenic 30.0%
AGAGAATATTGTGACTGATT+TGG + tig0020545:1229-1248 Msa1382960:CDS 30.0%
TGAAAGCAAAATGATGACAA+AGG - tig0020545:1356-1375 None:intergenic 30.0%
! TTGCTTTCATGCCTATTTTA+AGG + tig0020545:1367-1386 Msa1382960:three_prime_UTR 30.0%
!!! GGAGTACTATTTTGCTATTT+TGG - tig0020545:1444-1463 None:intergenic 30.0%
TTTCCAATCATAGTTCGAGA+AGG + tig0020545:25-44 Msa1382960:five_prime_UTR 35.0%
! AAGGCATCATCTGCAATATT+AGG + tig0020545:44-63 Msa1382960:five_prime_UTR 35.0%
AGGCATCATCTGCAATATTA+GGG + tig0020545:45-64 Msa1382960:five_prime_UTR 35.0%
AATGGGAAGATTAGAGTTAG+AGG + tig0020545:166-185 Msa1382960:five_prime_UTR 35.0%
! GCTTACTTCTCCATTTGTTT+TGG - tig0020545:197-216 None:intergenic 35.0%
ACTAGTAGTGATAACCCTAT+TGG + tig0020545:455-474 Msa1382960:five_prime_UTR 35.0%
CTAGTAGTGATAACCCTATT+GGG + tig0020545:456-475 Msa1382960:five_prime_UTR 35.0%
TAGTAGTGATAACCCTATTG+GGG + tig0020545:457-476 Msa1382960:five_prime_UTR 35.0%
TAACGTCAACCAATGATCAA+AGG - tig0020545:561-580 None:intergenic 35.0%
CTTCATTCCTATTCCTTGAT+AGG - tig0020545:654-673 None:intergenic 35.0%
TCAAGGAATAGGAATGAAGA+TGG + tig0020545:655-674 Msa1382960:five_prime_UTR 35.0%
ATGGAGGAAAATCTCAACAA+AGG + tig0020545:674-693 Msa1382960:five_prime_UTR 35.0%
GCTTTATATCACATGACAAG+TGG + tig0020545:1389-1408 Msa1382960:three_prime_UTR 35.0%
TCGAACTATGATTGGAAACA+AGG - tig0020545:23-42 None:intergenic 35.0%
TTTCCAATCATAGTTCGAGA+AGG + tig0020545:25-44 Msa1382960:five_prime_UTR 35.0%
! AAGGCATCATCTGCAATATT+AGG + tig0020545:44-63 Msa1382960:five_prime_UTR 35.0%
AGGCATCATCTGCAATATTA+GGG + tig0020545:45-64 Msa1382960:five_prime_UTR 35.0%
AATGGGAAGATTAGAGTTAG+AGG + tig0020545:166-185 Msa1382960:five_prime_UTR 35.0%
! GCTTACTTCTCCATTTGTTT+TGG - tig0020545:197-216 None:intergenic 35.0%
ACTAGTAGTGATAACCCTAT+TGG + tig0020545:455-474 Msa1382960:five_prime_UTR 35.0%
CTAGTAGTGATAACCCTATT+GGG + tig0020545:456-475 Msa1382960:five_prime_UTR 35.0%
TAGTAGTGATAACCCTATTG+GGG + tig0020545:457-476 Msa1382960:five_prime_UTR 35.0%
TAACGTCAACCAATGATCAA+AGG - tig0020545:561-580 None:intergenic 35.0%
CTTCATTCCTATTCCTTGAT+AGG - tig0020545:654-673 None:intergenic 35.0%
TCAAGGAATAGGAATGAAGA+TGG + tig0020545:655-674 Msa1382960:five_prime_UTR 35.0%
ATGGAGGAAAATCTCAACAA+AGG + tig0020545:674-693 Msa1382960:five_prime_UTR 35.0%
GCTTTATATCACATGACAAG+TGG + tig0020545:1389-1408 Msa1382960:three_prime_UTR 35.0%
ATGCCTTCTCGAACTATGAT+TGG - tig0020545:31-50 None:intergenic 40.0%
TGAGTAGAAGGGTTATAAGC+AGG + tig0020545:126-145 Msa1382960:five_prime_UTR 40.0%
AGAGGTAGAGCCAAAACAAA+TGG + tig0020545:184-203 Msa1382960:five_prime_UTR 40.0%
AAGCATCTGCAATAACCTCA+TGG + tig0020545:213-232 Msa1382960:five_prime_UTR 40.0%
AGCATCTGCAATAACCTCAT+GGG + tig0020545:214-233 Msa1382960:five_prime_UTR 40.0%
AATTCCGATAGATTTGGGAG+TGG + tig0020545:368-387 Msa1382960:five_prime_UTR 40.0%
ATTCCGATAGATTTGGGAGT+GGG + tig0020545:369-388 Msa1382960:five_prime_UTR 40.0%
AGGTAGCATAAACTTACACC+CGG + tig0020545:413-432 Msa1382960:five_prime_UTR 40.0%
TCTTAGTCTCTACAGTTACC+GGG - tig0020545:434-453 None:intergenic 40.0%
CTCTTAGTCTCTACAGTTAC+CGG - tig0020545:435-454 None:intergenic 40.0%
AGGAATAGGAATGAAGATGG+AGG + tig0020545:658-677 Msa1382960:five_prime_UTR 40.0%
GTCCAAAGACACAACTATAG+AGG + tig0020545:752-771 Msa1382960:five_prime_UTR 40.0%
AGCCTCTATAGTTGTGTCTT+TGG - tig0020545:757-776 None:intergenic 40.0%
!!! AGTGCTCTCTAGTTCTTTTC+AGG - tig0020545:987-1006 None:intergenic 40.0%
!! AAAGAACTAGAGAGCACTCA+AGG + tig0020545:989-1008 Msa1382960:CDS 40.0%
!! AAGAACTAGAGAGCACTCAA+GGG + tig0020545:990-1009 Msa1382960:CDS 40.0%
TGTAGTTGAAACAAGCGTGA+AGG + tig0020545:1026-1045 Msa1382960:CDS 40.0%
ATTGAGATGTAGCTGTTGCT+TGG + tig0020545:1328-1347 Msa1382960:three_prime_UTR 40.0%
ATGCCTTCTCGAACTATGAT+TGG - tig0020545:31-50 None:intergenic 40.0%
TGAGTAGAAGGGTTATAAGC+AGG + tig0020545:126-145 Msa1382960:five_prime_UTR 40.0%
AGAGGTAGAGCCAAAACAAA+TGG + tig0020545:184-203 Msa1382960:five_prime_UTR 40.0%
AAGCATCTGCAATAACCTCA+TGG + tig0020545:213-232 Msa1382960:five_prime_UTR 40.0%
AGCATCTGCAATAACCTCAT+GGG + tig0020545:214-233 Msa1382960:five_prime_UTR 40.0%
AATTCCGATAGATTTGGGAG+TGG + tig0020545:368-387 Msa1382960:five_prime_UTR 40.0%
ATTCCGATAGATTTGGGAGT+GGG + tig0020545:369-388 Msa1382960:five_prime_UTR 40.0%
AGGTAGCATAAACTTACACC+CGG + tig0020545:413-432 Msa1382960:five_prime_UTR 40.0%
TCTTAGTCTCTACAGTTACC+GGG - tig0020545:434-453 None:intergenic 40.0%
CTCTTAGTCTCTACAGTTAC+CGG - tig0020545:435-454 None:intergenic 40.0%
AGGAATAGGAATGAAGATGG+AGG + tig0020545:658-677 Msa1382960:five_prime_UTR 40.0%
GTCCAAAGACACAACTATAG+AGG + tig0020545:752-771 Msa1382960:five_prime_UTR 40.0%
AGCCTCTATAGTTGTGTCTT+TGG - tig0020545:757-776 None:intergenic 40.0%
!!! AGTGCTCTCTAGTTCTTTTC+AGG - tig0020545:987-1006 None:intergenic 40.0%
!! AAAGAACTAGAGAGCACTCA+AGG + tig0020545:989-1008 Msa1382960:CDS 40.0%
!! AAGAACTAGAGAGCACTCAA+GGG + tig0020545:990-1009 Msa1382960:CDS 40.0%
TGTAGTTGAAACAAGCGTGA+AGG + tig0020545:1026-1045 Msa1382960:CDS 40.0%
ATTGAGATGTAGCTGTTGCT+TGG + tig0020545:1328-1347 Msa1382960:three_prime_UTR 40.0%
TCACGATGATAGACTTGCGA+AGG + tig0020545:280-299 Msa1382960:five_prime_UTR 45.0%
TTCCGATAGATTTGGGAGTG+GGG + tig0020545:370-389 Msa1382960:five_prime_UTR 45.0%
TTCCCCACTCCCAAATCTAT+CGG - tig0020545:375-394 None:intergenic 45.0%
! TAGTGTGTTGCTGCCTATCA+AGG + tig0020545:638-657 Msa1382960:five_prime_UTR 45.0%
GTTGCTGCCTATCAAGGAAT+AGG + tig0020545:644-663 Msa1382960:five_prime_UTR 45.0%
AGACACAACTATAGAGGCTC+TGG + tig0020545:758-777 Msa1382960:five_prime_UTR 45.0%
TGCATTGTTGTGCAATCCTC+AGG + tig0020545:850-869 Msa1382960:five_prime_UTR 45.0%
!! TAATGCTGGCTTATCATGCC+AGG + tig0020545:957-976 Msa1382960:CDS 45.0%
!!! TAGTTCTTTTCAGGTCCTCC+TGG - tig0020545:978-997 None:intergenic 45.0%
CCAGTTCGTTTGTTTGAGTG+CGG + tig0020545:1462-1481 Msa1382960:three_prime_UTR 45.0%
TCACGATGATAGACTTGCGA+AGG + tig0020545:280-299 Msa1382960:five_prime_UTR 45.0%
TTCCGATAGATTTGGGAGTG+GGG + tig0020545:370-389 Msa1382960:five_prime_UTR 45.0%
TTCCCCACTCCCAAATCTAT+CGG - tig0020545:375-394 None:intergenic 45.0%
! TAGTGTGTTGCTGCCTATCA+AGG + tig0020545:638-657 Msa1382960:five_prime_UTR 45.0%
GTTGCTGCCTATCAAGGAAT+AGG + tig0020545:644-663 Msa1382960:five_prime_UTR 45.0%
AGACACAACTATAGAGGCTC+TGG + tig0020545:758-777 Msa1382960:five_prime_UTR 45.0%
TGCATTGTTGTGCAATCCTC+AGG + tig0020545:850-869 Msa1382960:five_prime_UTR 45.0%
!! TAATGCTGGCTTATCATGCC+AGG + tig0020545:957-976 Msa1382960:CDS 45.0%
!!! TAGTTCTTTTCAGGTCCTCC+TGG - tig0020545:978-997 None:intergenic 45.0%
CCAGTTCGTTTGTTTGAGTG+CGG + tig0020545:1462-1481 Msa1382960:three_prime_UTR 45.0%
GCAATAACCTCATGGGCAGA+AGG + tig0020545:221-240 Msa1382960:five_prime_UTR 50.0%
ACTTACTCCTTCTGCCCATG+AGG - tig0020545:231-250 None:intergenic 50.0%
TGGGCAGAAGGAGTAAGTCT+AGG + tig0020545:233-252 Msa1382960:five_prime_UTR 50.0%
GAAGGAGTAAGTCTAGGCTG+CGG + tig0020545:239-258 Msa1382960:five_prime_UTR 50.0%
GAACACGAGGCAGACATGAA+TGG + tig0020545:322-341 Msa1382960:five_prime_UTR 50.0%
! TGAATGGACTCAGAGCGAAC+AGG + tig0020545:338-357 Msa1382960:five_prime_UTR 50.0%
ACTCAGAGCGAACAGGCTAA+AGG + tig0020545:345-364 Msa1382960:five_prime_UTR 50.0%
AGTGATAACCCTATTGGGGC+TGG + tig0020545:461-480 Msa1382960:five_prime_UTR 50.0%
GTGATAACCCTATTGGGGCT+GGG + tig0020545:462-481 Msa1382960:five_prime_UTR 50.0%
GCTGGGTGTTCCAACATCTA+TGG + tig0020545:479-498 Msa1382960:five_prime_UTR 50.0%
!! TAGAGAGCACTCAAGGGTCT+CGG + tig0020545:996-1015 Msa1382960:CDS 50.0%
!! GACTGATTTGGCCAGAGAGA+CGG + tig0020545:1241-1260 Msa1382960:CDS 50.0%
TCACTTCAGATCCGTCTCTC+TGG - tig0020545:1255-1274 None:intergenic 50.0%
CCGCACTCAAACAAACGAAC+TGG - tig0020545:1465-1484 None:intergenic 50.0%
GCAATAACCTCATGGGCAGA+AGG + tig0020545:221-240 Msa1382960:five_prime_UTR 50.0%
ACTTACTCCTTCTGCCCATG+AGG - tig0020545:231-250 None:intergenic 50.0%
TGGGCAGAAGGAGTAAGTCT+AGG + tig0020545:233-252 Msa1382960:five_prime_UTR 50.0%
GAAGGAGTAAGTCTAGGCTG+CGG + tig0020545:239-258 Msa1382960:five_prime_UTR 50.0%
GAACACGAGGCAGACATGAA+TGG + tig0020545:322-341 Msa1382960:five_prime_UTR 50.0%
! TGAATGGACTCAGAGCGAAC+AGG + tig0020545:338-357 Msa1382960:five_prime_UTR 50.0%
ACTCAGAGCGAACAGGCTAA+AGG + tig0020545:345-364 Msa1382960:five_prime_UTR 50.0%
AGTGATAACCCTATTGGGGC+TGG + tig0020545:461-480 Msa1382960:five_prime_UTR 50.0%
GTGATAACCCTATTGGGGCT+GGG + tig0020545:462-481 Msa1382960:five_prime_UTR 50.0%
GCTGGGTGTTCCAACATCTA+TGG + tig0020545:479-498 Msa1382960:five_prime_UTR 50.0%
!! TAGAGAGCACTCAAGGGTCT+CGG + tig0020545:996-1015 Msa1382960:CDS 50.0%
!! GACTGATTTGGCCAGAGAGA+CGG + tig0020545:1241-1260 Msa1382960:CDS 50.0%
TCACTTCAGATCCGTCTCTC+TGG - tig0020545:1255-1274 None:intergenic 50.0%
CCGCACTCAAACAAACGAAC+TGG - tig0020545:1465-1484 None:intergenic 50.0%
GTAAGTCTAGGCTGCGGTGT+TGG + tig0020545:245-264 Msa1382960:five_prime_UTR 55.0%
TGGAACACCCAGCCCCAATA+GGG - tig0020545:472-491 None:intergenic 55.0%
TTGGAACACCCAGCCCCAAT+AGG - tig0020545:473-492 None:intergenic 55.0%
!! TGCTGGCTTATCATGCCAGG+AGG + tig0020545:960-979 Msa1382960:CDS 55.0%
GTAAGTCTAGGCTGCGGTGT+TGG + tig0020545:245-264 Msa1382960:five_prime_UTR 55.0%
TGGAACACCCAGCCCCAATA+GGG - tig0020545:472-491 None:intergenic 55.0%
TTGGAACACCCAGCCCCAAT+AGG - tig0020545:473-492 None:intergenic 55.0%
!! TGCTGGCTTATCATGCCAGG+AGG + tig0020545:960-979 Msa1382960:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0020545 gene 21 1558 21 ID=Msa1382960;Name=Msa1382960
tig0020545 mRNA 21 1558 21 ID=Msa1382960-mRNA-1;Parent=Msa1382960;Name=Msa1382960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=916|1|1|1|1|1|2|284|68
tig0020545 exon 21 1047 21 ID=Msa1382960-mRNA-1:exon:8394;Parent=Msa1382960-mRNA-1
tig0020545 exon 1179 1558 1179 ID=Msa1382960-mRNA-1:exon:8395;Parent=Msa1382960-mRNA-1
tig0020545 five_prime_UTR 21 936 21 ID=Msa1382960-mRNA-1:five_prime_utr;Parent=Msa1382960-mRNA-1
tig0020545 CDS 937 1047 937 ID=Msa1382960-mRNA-1:cds;Parent=Msa1382960-mRNA-1
tig0020545 CDS 1179 1274 1179 ID=Msa1382960-mRNA-1:cds;Parent=Msa1382960-mRNA-1
tig0020545 three_prime_UTR 1275 1558 1275 ID=Msa1382960-mRNA-1:three_prime_utr;Parent=Msa1382960-mRNA-1
Gene Sequence

>Msa1382960

ATGGAAAATTATACAATTTGTAATGCTGGCTTATCATGCCAGGAGGACCTGAAAAGAACTAGAGAGCACTCAAGGGTCTCGGATACTGCTGTAGTTGAAACAAGCGTGAAGGCTTTAGATATTTGTTCTCAGCCAGTTGATGAAGATAATGTGAAAGATGAAGAGAATATTGTGACTGATTTGGCCAGAGAGACGGATCTGAAGTGA
Protein sequence

>Msa1382960

MENYTICNAGLSCQEDLKRTREHSRVSDTAVVETSVKALDICSQPVDEDNVKDEENIVTDLARETDLK*