AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0606720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0606720 MtrunA17_Chr4g0010411 97.163 141 4 0 1 141 65 205 7.02e-96 275
Msa0606720 MtrunA17_Chr2g0308511 73.239 142 34 3 1 141 297 435 2.14e-60 192
Msa0606720 MtrunA17_Chr1g0175141 52.817 142 63 4 1 140 198 337 6.42e-39 134
Msa0606720 MtrunA17_Chr4g0056131 61.905 42 16 0 84 125 16 57 1.29e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0606720 AT2G44440.1 50.000 132 60 4 1 131 303 429 1.52e-29 111
Msa0606720 AT5G13020.1 51.938 129 51 6 1 125 273 394 1.95e-27 105
Msa0606720 AT5G06780.1 48.438 128 59 3 1 127 186 307 7.00e-27 102
Msa0606720 AT5G06780.2 48.438 128 59 3 1 127 179 300 7.10e-27 102
Msa0606720 AT3G12140.2 47.761 134 57 4 1 127 187 314 3.03e-20 84.7
Msa0606720 AT3G12140.1 47.761 134 57 4 1 127 187 314 3.03e-20 84.7
Msa0606720 AT3G12140.3 47.328 131 56 4 1 124 187 311 9.50e-19 81.3

Find 62 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAGCTATAAATTGTTAAA+AGG 0.227179 4_2:-53333471 None:intergenic
GTGTCGAAATTCTTGTATTA+GGG 0.232000 4_2:+53333654 Msa0606720:three_prime_UTR
TGTGTCGAAATTCTTGTATT+AGG 0.240285 4_2:+53333653 Msa0606720:three_prime_UTR
TAGCCATGTTCTTGTGGATT+AGG 0.291206 4_2:+53333492 Msa0606720:three_prime_UTR
GAAAGCGAAGAAAGTATTAA+AGG 0.307793 4_2:+53331529 Msa0606720:CDS
GGATGGCAATTGTTGTAGTT+TGG 0.361143 4_2:+53333524 Msa0606720:three_prime_UTR
AATGAGAAGATAAGGCATTA+TGG 0.391675 4_2:+53333388 Msa0606720:three_prime_UTR
TCTTCGCTTTCTCAATTTCA+AGG 0.392109 4_2:-53331517 None:intergenic
ACACACAGACACACTAATAA+AGG 0.400164 4_2:+53331365 Msa0606720:CDS
TTCTCAATTTCAAGGGGATC+AGG 0.400539 4_2:-53331509 None:intergenic
CTTCGCTTTCTCAATTTCAA+GGG 0.434239 4_2:-53331516 None:intergenic
CCAGGAGTTGGAAGAGGTAG+AGG 0.441154 4_2:+53331261 Msa0606720:CDS
TTGTGGATTAGGTATTATCT+AGG 0.451013 4_2:+53333503 Msa0606720:three_prime_UTR
CTTTGTACAATGAGAAGATA+AGG 0.456419 4_2:+53333380 Msa0606720:three_prime_UTR
TCTACCAACTGAATATCTTC+AGG 0.459149 4_2:-53331153 None:intergenic
TAGCTCTAGCCATGTTCTTG+TGG 0.461777 4_2:+53333486 Msa0606720:three_prime_UTR
AGAGCTATAAATTGTTAAAA+GGG 0.464396 4_2:-53333470 None:intergenic
GTCCTGGTCATGGGATGAAT+AGG 0.465538 4_2:+53331217 Msa0606720:CDS
CCTCTACCTCTTCCAACTCC+TGG 0.466468 4_2:-53331261 None:intergenic
CCGTGTGGAAAACGGTGGCC+AGG 0.470228 4_2:-53333321 None:intergenic
CGTGAGAGTGGTCCAGGAGT+TGG 0.470322 4_2:+53331249 Msa0606720:CDS
TAGATCGACCGTGTGGAAAA+CGG 0.476086 4_2:-53333329 None:intergenic
GTAGGGCGTGAGAGTGGTCC+AGG 0.486551 4_2:+53331243 Msa0606720:CDS
TTCTTGTATTAGGGGTTGCA+TGG 0.489250 4_2:+53333663 Msa0606720:three_prime_UTR
TTGTCATCACAGAATGGCAT+AGG 0.516282 4_2:+53331315 Msa0606720:CDS
ATTGTTGATACAGATGGGCC+TGG 0.522652 4_2:+53333303 Msa0606720:intron
AGATATTCAGTTGGTAGAAG+AGG 0.538459 4_2:+53331158 Msa0606720:CDS
TTCGCTTTCTCAATTTCAAG+GGG 0.538679 4_2:-53331515 None:intergenic
TTGGATTTCACAGGTGGAGA+GGG 0.542144 4_2:+53331469 Msa0606720:intron
TGTAGTATCTGAATATCATC+GGG 0.543121 4_2:-53331345 None:intergenic
ATCCTGAAAGTAATCATCGC+GGG 0.550186 4_2:+53331184 Msa0606720:CDS
CAGGAGTTGGAAGAGGTAGA+GGG 0.551041 4_2:+53331262 Msa0606720:CDS
GCATTATGGTAGCATAACTC+TGG 0.551170 4_2:+53333402 Msa0606720:three_prime_UTR
GTGTAGTATCTGAATATCAT+CGG 0.551674 4_2:-53331346 None:intergenic
CATGGGATGAATAGGTCTGT+AGG 0.560661 4_2:+53331225 Msa0606720:CDS
TATTCAGTTGGTAGAAGAGG+AGG 0.562816 4_2:+53331161 Msa0606720:CDS
AGTGGTCCAGGAGTTGGAAG+AGG 0.563371 4_2:+53331255 Msa0606720:CDS
AAGAGGTAGAGGGGTTCCAA+AGG 0.564857 4_2:+53331272 Msa0606720:CDS
AGAGGTAGAGGGGTTCCAAA+GGG 0.565630 4_2:+53331273 Msa0606720:CDS
GGATTAGGTATTATCTAGGA+TGG 0.565666 4_2:+53333507 Msa0606720:three_prime_UTR
ATGGGATGAATAGGTCTGTA+GGG 0.581808 4_2:+53331226 Msa0606720:CDS
ATCTCCTGAAGATATTCAGT+TGG 0.582166 4_2:+53331149 Msa0606720:CDS
GACCTATTCATCCCATGACC+AGG 0.584806 4_2:-53331219 None:intergenic
GTTGGATTTCACAGGTGGAG+AGG 0.587138 4_2:+53331468 Msa0606720:intron
GATCTCTCTGATGGTGAAAG+TGG 0.588196 4_2:+53331698 Msa0606720:CDS
CTTATCTTCTCATTGTACAA+AGG 0.594344 4_2:-53333379 None:intergenic
ACTTTCACCATCAGAGAGAT+CGG 0.607034 4_2:-53331696 None:intergenic
ATCACAGAATGGCATAGGAA+AGG 0.610087 4_2:+53331320 Msa0606720:CDS
TAATTCGATGCAGGATCATG+AGG 0.616423 4_2:+53331643 Msa0606720:intron
AGACTTACCGATCTCTCTGA+TGG 0.617087 4_2:+53331689 Msa0606720:CDS
ATACCTAATCCACAAGAACA+TGG 0.625316 4_2:-53333495 None:intergenic
TCCTGAAAGTAATCATCGCG+GGG 0.625668 4_2:+53331185 Msa0606720:CDS
TGTCGAAATTCTTGTATTAG+GGG 0.631694 4_2:+53333655 Msa0606720:three_prime_UTR
AGGTCTGTAGGGCGTGAGAG+TGG 0.633155 4_2:+53331237 Msa0606720:CDS
TCGAGTTTAAAATTTGACGT+AGG 0.650719 4_2:+53333560 Msa0606720:three_prime_UTR
TTCGATGCAGGATCATGAGG+AGG 0.651732 4_2:+53331646 Msa0606720:intron
CACAGACACACTAATAAAGG+AGG 0.653674 4_2:+53331368 Msa0606720:CDS
AGGAGTTGGAAGAGGTAGAG+GGG 0.655509 4_2:+53331263 Msa0606720:CDS
CGATCCATAGATCGACCGTG+TGG 0.696183 4_2:-53333336 None:intergenic
GATCCTGAAAGTAATCATCG+CGG 0.726995 4_2:+53331183 Msa0606720:CDS
CCTGAAAGTAATCATCGCGG+GGG 0.730541 4_2:+53331186 Msa0606720:CDS
ATCGACCGTGTGGAAAACGG+TGG 0.751262 4_2:-53333326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTTAATAAACTTTT+TGG + chr4_2:53332055-53332074 Msa0606720:intron 10.0%
!! AAACATACAATCTTAATAAT+TGG + chr4_2:53331923-53331942 Msa0606720:intron 15.0%
!!! TTTTAACTATAAACATAAAG+TGG + chr4_2:53331958-53331977 Msa0606720:intron 15.0%
!! TTTGTCAAACAAAAAAAAAA+AGG + chr4_2:53332690-53332709 Msa0606720:intron 15.0%
!!! TTTGATTTTCTTGCAAAAAA+TGG + chr4_2:53331593-53331612 Msa0606720:intron 20.0%
!! GAATTATATATAGATAGATC+AGG - chr4_2:53331629-53331648 None:intergenic 20.0%
!!! TTAGTTGCATTAATTAGTTT+TGG + chr4_2:53331786-53331805 Msa0606720:intron 20.0%
!!! TTCATTCTAGTTATTATCTT+TGG - chr4_2:53331884-53331903 None:intergenic 20.0%
!! TACAATCTTAATAATTGGTT+TGG + chr4_2:53331928-53331947 Msa0606720:intron 20.0%
!! TTTATTAAAAAAGAAGCGAA+GGG - chr4_2:53332050-53332069 None:intergenic 20.0%
!!! AATCAGTTTTAGTCTTTTTT+GGG - chr4_2:53332091-53332110 None:intergenic 20.0%
!!! AGCAAATATTTTCTGATATT+GGG - chr4_2:53332218-53332237 None:intergenic 20.0%
!! ATATCAAGAATAGAGATAAT+AGG - chr4_2:53332285-53332304 None:intergenic 20.0%
!! AAGCAACTTCTATTTATTTA+TGG - chr4_2:53332455-53332474 None:intergenic 20.0%
!!! TTTTTGAAATTCTTGAAGTT+GGG - chr4_2:53333012-53333031 None:intergenic 20.0%
!! TTGTATCAAAATATATGAAG+TGG + chr4_2:53333060-53333079 Msa0606720:intron 20.0%
!! AGAGCTATAAATTGTTAAAA+GGG - chr4_2:53333473-53333492 None:intergenic 20.0%
!! TAGAGCTATAAATTGTTAAA+AGG - chr4_2:53333474-53333493 None:intergenic 20.0%
! CCAACAGAAACTTAAATATA+CGG - chr4_2:53331453-53331472 None:intergenic 25.0%
! TTTCACTATCAAAATGGAAT+AGG - chr4_2:53331561-53331580 None:intergenic 25.0%
!!! ATTCCATTTTGATAGTGAAA+AGG + chr4_2:53331561-53331580 Msa0606720:intron 25.0%
! TTGCAAGAAAATCAAATAGT+CGG - chr4_2:53331589-53331608 None:intergenic 25.0%
! ATCTATATATAATTCGATGC+AGG + chr4_2:53331634-53331653 Msa0606720:intron 25.0%
! AAGAAGACTAAACAATAACA+TGG - chr4_2:53331768-53331787 None:intergenic 25.0%
!! ATTCTAGTTATTATCTTTGG+TGG - chr4_2:53331881-53331900 None:intergenic 25.0%
! GTTTATTAAAAAAGAAGCGA+AGG - chr4_2:53332051-53332070 None:intergenic 25.0%
!!! CAATCAGTTTTAGTCTTTTT+TGG - chr4_2:53332092-53332111 None:intergenic 25.0%
! CAAAAAAGACTAAAACTGAT+TGG + chr4_2:53332090-53332109 Msa0606720:intron 25.0%
! AAGGAAAAATTGGATGAATA+GGG - chr4_2:53332126-53332145 None:intergenic 25.0%
!! TCCAATTTTTCCTTCTTTTT+TGG + chr4_2:53332132-53332151 Msa0606720:intron 25.0%
! ACCAAAAAAGAAGGAAAAAT+TGG - chr4_2:53332136-53332155 None:intergenic 25.0%
!! GCAAATATTTTCTGATATTG+GGG - chr4_2:53332217-53332236 None:intergenic 25.0%
!! CAGCAAATATTTTCTGATAT+TGG - chr4_2:53332219-53332238 None:intergenic 25.0%
!! ACTTGTTAACATTTGTCTAT+TGG + chr4_2:53332602-53332621 Msa0606720:intron 25.0%
!!! TTTTTGTTTGACAAATCCTA+TGG - chr4_2:53332685-53332704 None:intergenic 25.0%
! TAAAAACAAGGAACAATTGA+AGG - chr4_2:53332760-53332779 None:intergenic 25.0%
! TTCATTCACTCATAAAAACA+AGG - chr4_2:53332772-53332791 None:intergenic 25.0%
! TTGATCATGTATGTCTATTT+GGG - chr4_2:53332933-53332952 None:intergenic 25.0%
!!! GTTTTTGAAATTCTTGAAGT+TGG - chr4_2:53333013-53333032 None:intergenic 25.0%
!!! TGTTTTATTGTTGATACAGA+TGG + chr4_2:53333297-53333316 Msa0606720:intron 25.0%
!!! GTTTTATTGTTGATACAGAT+GGG + chr4_2:53333298-53333317 Msa0606720:intron 25.0%
!! AATTTTAAACAAGTATCAGC+TGG - chr4_2:53333587-53333606 None:intergenic 25.0%
!!! GATTTTTTGTCATCACAGAA+TGG + chr4_2:53331309-53331328 Msa0606720:CDS 30.0%
TGTAGTATCTGAATATCATC+GGG - chr4_2:53331348-53331367 None:intergenic 30.0%
GTGTAGTATCTGAATATCAT+CGG - chr4_2:53331349-53331368 None:intergenic 30.0%
! CCGTATATTTAAGTTTCTGT+TGG + chr4_2:53331450-53331469 Msa0606720:intron 30.0%
!! GAAAGCGAAGAAAGTATTAA+AGG + chr4_2:53331529-53331548 Msa0606720:CDS 30.0%
! AGCTCATGAGTATTTCTATA+AGG - chr4_2:53331742-53331761 None:intergenic 30.0%
!!! GCATTAATTAGTTTTGGAAC+TGG + chr4_2:53331792-53331811 Msa0606720:intron 30.0%
AAAATGCAAAAAATTGTGGC+CGG + chr4_2:53331983-53332002 Msa0606720:intron 30.0%
GAAGGAAAAATTGGATGAAT+AGG - chr4_2:53332127-53332146 None:intergenic 30.0%
! ATATTTTCTGATATTGGGGA+GGG - chr4_2:53332213-53332232 None:intergenic 30.0%
! AATATTTTCTGATATTGGGG+AGG - chr4_2:53332214-53332233 None:intergenic 30.0%
ATCTCTAACAGTCATACTAA+CGG - chr4_2:53332563-53332582 None:intergenic 30.0%
TAAAATCCTACTACAACTGT+TGG - chr4_2:53332721-53332740 None:intergenic 30.0%
! CTCTAGTTCATGAAATTGTA+AGG + chr4_2:53332795-53332814 Msa0606720:intron 30.0%
CTTGATCATGTATGTCTATT+TGG - chr4_2:53332934-53332953 None:intergenic 30.0%
TTCTTGAAGTTGGGTAATTA+AGG - chr4_2:53333003-53333022 None:intergenic 30.0%
! ACAAAAGTATAGTAGCTTGT+CGG - chr4_2:53333041-53333060 None:intergenic 30.0%
TTTCACAGCTATTACAATCA+CGG - chr4_2:53333112-53333131 None:intergenic 30.0%
! AAAGTTACTTTGTCTAACCT+TGG + chr4_2:53333272-53333291 Msa0606720:intron 30.0%
ATCAACAATAAAACACACCA+AGG - chr4_2:53333292-53333311 None:intergenic 30.0%
CTTATCTTCTCATTGTACAA+AGG - chr4_2:53333382-53333401 None:intergenic 30.0%
CTTTGTACAATGAGAAGATA+AGG + chr4_2:53333380-53333399 Msa0606720:three_prime_UTR 30.0%
! AATGAGAAGATAAGGCATTA+TGG + chr4_2:53333388-53333407 Msa0606720:three_prime_UTR 30.0%
! TAGCATAACTCTGGAAAAAA+GGG + chr4_2:53333411-53333430 Msa0606720:three_prime_UTR 30.0%
!! TTGTGGATTAGGTATTATCT+AGG + chr4_2:53333503-53333522 Msa0606720:three_prime_UTR 30.0%
! TCGAGTTTAAAATTTGACGT+AGG + chr4_2:53333560-53333579 Msa0606720:three_prime_UTR 30.0%
TGTGTCGAAATTCTTGTATT+AGG + chr4_2:53333653-53333672 Msa0606720:three_prime_UTR 30.0%
GTGTCGAAATTCTTGTATTA+GGG + chr4_2:53333654-53333673 Msa0606720:three_prime_UTR 30.0%
TGTCGAAATTCTTGTATTAG+GGG + chr4_2:53333655-53333674 Msa0606720:three_prime_UTR 30.0%
ATCTCCTGAAGATATTCAGT+TGG + chr4_2:53331149-53331168 Msa0606720:CDS 35.0%
TCTACCAACTGAATATCTTC+AGG - chr4_2:53331156-53331175 None:intergenic 35.0%
!! AGATATTCAGTTGGTAGAAG+AGG + chr4_2:53331158-53331177 Msa0606720:CDS 35.0%
! TCTTTTCTAGATTGACCCTT+TGG - chr4_2:53331291-53331310 None:intergenic 35.0%
ACACACAGACACACTAATAA+AGG + chr4_2:53331365-53331384 Msa0606720:CDS 35.0%
AAGTTTCTGTTGGATTTCAC+AGG + chr4_2:53331460-53331479 Msa0606720:intron 35.0%
TTCGCTTTCTCAATTTCAAG+GGG - chr4_2:53331518-53331537 None:intergenic 35.0%
CTTCGCTTTCTCAATTTCAA+GGG - chr4_2:53331519-53331538 None:intergenic 35.0%
TCTTCGCTTTCTCAATTTCA+AGG - chr4_2:53331520-53331539 None:intergenic 35.0%
! GCACCTTTTCACTATCAAAA+TGG - chr4_2:53331567-53331586 None:intergenic 35.0%
! AATACCACTGCAGCATTTTT+GGG - chr4_2:53331837-53331856 None:intergenic 35.0%
GGCCAAAATGCAAAAAATTG+TGG + chr4_2:53331979-53331998 Msa0606720:intron 35.0%
!!! GGCCACAATTTTTTGCATTT+TGG - chr4_2:53331984-53332003 None:intergenic 35.0%
! TTTTGTCGCACCAAAAAAGA+AGG - chr4_2:53332145-53332164 None:intergenic 35.0%
AGAATAGAGATAATAGGCAC+AGG - chr4_2:53332279-53332298 None:intergenic 35.0%
TGAGAATGACACCATAACAA+TGG - chr4_2:53332324-53332343 None:intergenic 35.0%
GTATTGTGCTAATTGTGGTT+GGG - chr4_2:53332349-53332368 None:intergenic 35.0%
AGTATTGTGCTAATTGTGGT+TGG - chr4_2:53332350-53332369 None:intergenic 35.0%
ACTCAGTATTGTGCTAATTG+TGG - chr4_2:53332354-53332373 None:intergenic 35.0%
CTCAAAATATGTCACAGAAC+CGG - chr4_2:53332388-53332407 None:intergenic 35.0%
CTTGTATCGTACCTAAACTT+TGG + chr4_2:53332472-53332491 Msa0606720:intron 35.0%
AGTATGACTGTTAGAGATGT+TGG + chr4_2:53332565-53332584 Msa0606720:intron 35.0%
GAGTTTCTGCAGATGTTATT+TGG + chr4_2:53332630-53332649 Msa0606720:intron 35.0%
! TTTCTGCAGATGTTATTTGG+TGG + chr4_2:53332633-53332652 Msa0606720:intron 35.0%
ACTAGAATCTAACAAGCCAT+AGG + chr4_2:53332666-53332685 Msa0606720:intron 35.0%
! GTAGCATAACTCTGGAAAAA+AGG + chr4_2:53333410-53333429 Msa0606720:three_prime_UTR 35.0%
ATACCTAATCCACAAGAACA+TGG - chr4_2:53333498-53333517 None:intergenic 35.0%
GGATTAGGTATTATCTAGGA+TGG + chr4_2:53333507-53333526 Msa0606720:three_prime_UTR 35.0%
!! TATTCAGTTGGTAGAAGAGG+AGG + chr4_2:53331161-53331180 Msa0606720:CDS 40.0%
GATCCTGAAAGTAATCATCG+CGG + chr4_2:53331183-53331202 Msa0606720:CDS 40.0%
ATCCTGAAAGTAATCATCGC+GGG + chr4_2:53331184-53331203 Msa0606720:CDS 40.0%
ATGGGATGAATAGGTCTGTA+GGG + chr4_2:53331226-53331245 Msa0606720:CDS 40.0%
TTGTCATCACAGAATGGCAT+AGG + chr4_2:53331315-53331334 Msa0606720:CDS 40.0%
ATCACAGAATGGCATAGGAA+AGG + chr4_2:53331320-53331339 Msa0606720:CDS 40.0%
CACAGACACACTAATAAAGG+AGG + chr4_2:53331368-53331387 Msa0606720:CDS 40.0%
AGACACACTAATAAAGGAGG+TGG + chr4_2:53331371-53331390 Msa0606720:intron 40.0%
TTTCTGTTGGATTTCACAGG+TGG + chr4_2:53331463-53331482 Msa0606720:intron 40.0%
TTCTCAATTTCAAGGGGATC+AGG - chr4_2:53331512-53331531 None:intergenic 40.0%
TAATTCGATGCAGGATCATG+AGG + chr4_2:53331643-53331662 Msa0606720:intron 40.0%
ACTTTCACCATCAGAGAGAT+CGG - chr4_2:53331699-53331718 None:intergenic 40.0%
ATGGTGAAAGTGGTACTTCT+TGG + chr4_2:53331708-53331727 Msa0606720:intron 40.0%
! ATACCACTGCAGCATTTTTG+GGG - chr4_2:53331836-53331855 None:intergenic 40.0%
! GAATACCACTGCAGCATTTT+TGG - chr4_2:53331838-53331857 None:intergenic 40.0%
AAAATGCTGCAGTGGTATTC+CGG + chr4_2:53331838-53331857 Msa0606720:intron 40.0%
AAGAAGCGAAGGGAATTTCT+GGG - chr4_2:53332040-53332059 None:intergenic 40.0%
AAAGAAGCGAAGGGAATTTC+TGG - chr4_2:53332041-53332060 None:intergenic 40.0%
CTGAATTCACAGAGTTTGGA+AGG - chr4_2:53332182-53332201 None:intergenic 40.0%
TTCTCAGTCTGCCATTGTTA+TGG + chr4_2:53332310-53332329 Msa0606720:intron 40.0%
AGCACAATACTGAGTTCTGT+TGG + chr4_2:53332359-53332378 Msa0606720:intron 40.0%
! TACTGAGTTCTGTTGGTTAC+CGG + chr4_2:53332366-53332385 Msa0606720:intron 40.0%
! GTACCTAAACTTTGGTTCAG+CGG + chr4_2:53332480-53332499 Msa0606720:intron 40.0%
TAACATCTGCAGAAACTCGA+AGG - chr4_2:53332628-53332647 None:intergenic 40.0%
! TGCAGATGTTATTTGGTGGA+TGG + chr4_2:53332637-53332656 Msa0606720:intron 40.0%
GATTGACCAACAGTTGTAGT+AGG + chr4_2:53332712-53332731 Msa0606720:intron 40.0%
AGCTATTACAATCACGGTGA+AGG - chr4_2:53333106-53333125 None:intergenic 40.0%
GCATTATGGTAGCATAACTC+TGG + chr4_2:53333402-53333421 Msa0606720:three_prime_UTR 40.0%
TAGCCATGTTCTTGTGGATT+AGG + chr4_2:53333492-53333511 Msa0606720:three_prime_UTR 40.0%
GGATGGCAATTGTTGTAGTT+TGG + chr4_2:53333524-53333543 Msa0606720:three_prime_UTR 40.0%
TTCTTGTATTAGGGGTTGCA+TGG + chr4_2:53333663-53333682 Msa0606720:three_prime_UTR 40.0%
TCCTGAAAGTAATCATCGCG+GGG + chr4_2:53331185-53331204 Msa0606720:CDS 45.0%
CATGGGATGAATAGGTCTGT+AGG + chr4_2:53331225-53331244 Msa0606720:CDS 45.0%
TTGGATTTCACAGGTGGAGA+GGG + chr4_2:53331469-53331488 Msa0606720:intron 45.0%
AGACTTACCGATCTCTCTGA+TGG + chr4_2:53331689-53331708 Msa0606720:CDS 45.0%
! GATCTCTCTGATGGTGAAAG+TGG + chr4_2:53331698-53331717 Msa0606720:CDS 45.0%
!!! GTTTTGGAACTGGTACAGTG+TGG + chr4_2:53331802-53331821 Msa0606720:intron 45.0%
! AGCGCTGAATTCACAGAGTT+TGG - chr4_2:53332186-53332205 None:intergenic 45.0%
ACGCTGCTGCAGTGTAAATA+TGG - chr4_2:53332506-53332525 None:intergenic 45.0%
! TTTACACTGCAGCAGCGTTT+TGG + chr4_2:53332508-53332527 Msa0606720:intron 45.0%
! TTACACTGCAGCAGCGTTTT+GGG + chr4_2:53332509-53332528 Msa0606720:intron 45.0%
!! TTTTGGGGACGTCTTCTATC+CGG + chr4_2:53332525-53332544 Msa0606720:intron 45.0%
GACTGTTAGAGATGTTGGTG+TGG + chr4_2:53332570-53332589 Msa0606720:intron 45.0%
TCGATCACATTAGTCCATGC+AGG + chr4_2:53332881-53332900 Msa0606720:intron 45.0%
AGTCCATGCAGGAGAATTCA+AGG + chr4_2:53332892-53332911 Msa0606720:intron 45.0%
GTCCATGCAGGAGAATTCAA+GGG + chr4_2:53332893-53332912 Msa0606720:intron 45.0%
ATTGTTGATACAGATGGGCC+TGG + chr4_2:53333303-53333322 Msa0606720:intron 45.0%
TAGATCGACCGTGTGGAAAA+CGG - chr4_2:53333332-53333351 None:intergenic 45.0%
! TTTTCCACACGGTCGATCTA+TGG + chr4_2:53333332-53333351 Msa0606720:CDS 45.0%
!! TAGCTCTAGCCATGTTCTTG+TGG + chr4_2:53333486-53333505 Msa0606720:three_prime_UTR 45.0%
!!! TTTTTTAATTTCTTTATTTT+AGG - chr4_2:53332837-53332856 None:intergenic 5.0%
CCTGAAAGTAATCATCGCGG+GGG + chr4_2:53331186-53331205 Msa0606720:CDS 50.0%
! AGTAATCATCGCGGGGGTTT+TGG + chr4_2:53331192-53331211 Msa0606720:CDS 50.0%
! GTAATCATCGCGGGGGTTTT+GGG + chr4_2:53331193-53331212 Msa0606720:CDS 50.0%
! TAATCATCGCGGGGGTTTTG+GGG + chr4_2:53331194-53331213 Msa0606720:CDS 50.0%
GTCCTGGTCATGGGATGAAT+AGG + chr4_2:53331217-53331236 Msa0606720:CDS 50.0%
GACCTATTCATCCCATGACC+AGG - chr4_2:53331222-53331241 None:intergenic 50.0%
CAGGAGTTGGAAGAGGTAGA+GGG + chr4_2:53331262-53331281 Msa0606720:CDS 50.0%
AGGAGTTGGAAGAGGTAGAG+GGG + chr4_2:53331263-53331282 Msa0606720:CDS 50.0%
AAGAGGTAGAGGGGTTCCAA+AGG + chr4_2:53331272-53331291 Msa0606720:CDS 50.0%
AGAGGTAGAGGGGTTCCAAA+GGG + chr4_2:53331273-53331292 Msa0606720:CDS 50.0%
GTTGGATTTCACAGGTGGAG+AGG + chr4_2:53331468-53331487 Msa0606720:intron 50.0%
TTCGATGCAGGATCATGAGG+AGG + chr4_2:53331646-53331665 Msa0606720:intron 50.0%
CAACCCCAAAAATGCTGCAG+TGG + chr4_2:53331830-53331849 Msa0606720:intron 50.0%
TGGTATTCCGGCTTACAGAG+TGG + chr4_2:53331850-53331869 Msa0606720:intron 50.0%
TGCATCATCACTGTAGCAGC+TGG - chr4_2:53332010-53332029 None:intergenic 50.0%
TGGCCGCTGAACCAAAGTTT+AGG - chr4_2:53332486-53332505 None:intergenic 50.0%
! TACACTGCAGCAGCGTTTTG+GGG + chr4_2:53332510-53332529 Msa0606720:intron 50.0%
! CTAACGGATGTAGTCAGTGC+CGG - chr4_2:53332547-53332566 None:intergenic 50.0%
GTCCCTTGAATTCTCCTGCA+TGG - chr4_2:53332898-53332917 None:intergenic 50.0%
CCCCCGCGATGATTACTTTC+AGG - chr4_2:53331189-53331208 None:intergenic 55.0%
!! AATCATCGCGGGGGTTTTGG+GGG + chr4_2:53331195-53331214 Msa0606720:CDS 55.0%
!!! GTTTTGGGGGTCCTGGTCAT+GGG + chr4_2:53331208-53331227 Msa0606720:CDS 55.0%
AGTGGTCCAGGAGTTGGAAG+AGG + chr4_2:53331255-53331274 Msa0606720:CDS 55.0%
CCTCTACCTCTTCCAACTCC+TGG - chr4_2:53331264-53331283 None:intergenic 55.0%
CCAGGAGTTGGAAGAGGTAG+AGG + chr4_2:53331261-53331280 Msa0606720:CDS 55.0%
GGAGCAACCACTCTGTAAGC+CGG - chr4_2:53331860-53331879 None:intergenic 55.0%
CATCACTGTAGCAGCTGGTC+CGG - chr4_2:53332005-53332024 None:intergenic 55.0%
TCACAGAGTTTGGAAGGCCG+TGG - chr4_2:53332176-53332195 None:intergenic 55.0%
TAGGCACAGGAACAGAGCAC+AGG - chr4_2:53332266-53332285 None:intergenic 55.0%
ATCGACCGTGTGGAAAACGG+TGG - chr4_2:53333329-53333348 None:intergenic 55.0%
CGATCCATAGATCGACCGTG+TGG - chr4_2:53333339-53333358 None:intergenic 55.0%
!!! GGTTTTGGGGGTCCTGGTCA+TGG + chr4_2:53331207-53331226 Msa0606720:CDS 60.0%
AGGTCTGTAGGGCGTGAGAG+TGG + chr4_2:53331237-53331256 Msa0606720:CDS 60.0%
CGTGAGAGTGGTCCAGGAGT+TGG + chr4_2:53331249-53331268 Msa0606720:CDS 60.0%
GCGACAAAACCCAGCAGCCA+CGG + chr4_2:53332156-53332175 Msa0606720:intron 60.0%
TTTGGAAGGCCGTGGCTGCT+GGG - chr4_2:53332168-53332187 None:intergenic 60.0%
! CCTGGCCACCGTTTTCCACA+CGG + chr4_2:53333321-53333340 Msa0606720:CDS 60.0%
GTAGGGCGTGAGAGTGGTCC+AGG + chr4_2:53331243-53331262 Msa0606720:CDS 65.0%
GTTTGGAAGGCCGTGGCTGC+TGG - chr4_2:53332169-53332188 None:intergenic 65.0%
CCGTGTGGAAAACGGTGGCC+AGG - chr4_2:53333324-53333343 None:intergenic 65.0%
!!! CGCGGGGGTTTTGGGGGTCC+TGG + chr4_2:53331201-53331220 Msa0606720:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 53331147 53333712 53331147 ID=Msa0606720;Name=Msa0606720
chr4_2 mRNA 53331147 53333712 53331147 ID=Msa0606720-mRNA-1;Parent=Msa0606720;Name=Msa0606720-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.75|1|1|1|4|347|141
chr4_2 exon 53331147 53331389 53331147 ID=Msa0606720-mRNA-1:exon:13101;Parent=Msa0606720-mRNA-1
chr4_2 exon 53331482 53331550 53331482 ID=Msa0606720-mRNA-1:exon:13102;Parent=Msa0606720-mRNA-1
chr4_2 exon 53331656 53331719 53331656 ID=Msa0606720-mRNA-1:exon:13103;Parent=Msa0606720-mRNA-1
chr4_2 exon 53333316 53333712 53333316 ID=Msa0606720-mRNA-1:exon:13104;Parent=Msa0606720-mRNA-1
chr4_2 CDS 53331147 53331389 53331147 ID=Msa0606720-mRNA-1:cds;Parent=Msa0606720-mRNA-1
chr4_2 CDS 53331482 53331550 53331482 ID=Msa0606720-mRNA-1:cds;Parent=Msa0606720-mRNA-1
chr4_2 CDS 53331656 53331719 53331656 ID=Msa0606720-mRNA-1:cds;Parent=Msa0606720-mRNA-1
chr4_2 CDS 53333316 53333365 53333316 ID=Msa0606720-mRNA-1:cds;Parent=Msa0606720-mRNA-1
chr4_2 three_prime_UTR 53333366 53333712 53333366 ID=Msa0606720-mRNA-1:three_prime_utr;Parent=Msa0606720-mRNA-1
Gene Sequence

>Msa0606720

ATATCTCCTGAAGATATTCAGTTGGTAGAAGAGGAGGATCCTGAAAGTAATCATCGCGGGGGTTTTGGGGGTCCTGGTCATGGGATGAATAGGTCTGTAGGGCGTGAGAGTGGTCCAGGAGTTGGAAGAGGTAGAGGGGTTCCAAAGGGTCAATCTAGAAAAGATTTTTTGTCATCACAGAATGGCATAGGAAAGGCTCCCGATGATATTCAGATACTACACACAGACACACTAATAAAGGAGGTGGAGAGGGTATTTAATGCAAATCATCCTGATCCCCTTGAAATTGAGAAAGCGAAGAAAGTATTAAAGGATCATGAGGAGGCTCTTGTTGATGCGCTTGCAAGACTTACCGATCTCTCTGATGGTGAAAGTGATGGGCCTGGCCACCGTTTTCCACACGGTCGATCTATGGATCGAGAATGA
Protein sequence

>Msa0606720

ISPEDIQLVEEEDPESNHRGGFGGPGHGMNRSVGRESGPGVGRGRGVPKGQSRKDFLSSQNGIGKAPDDIQILHTDTLIKEVERVFNANHPDPLEIEKAKKVLKDHEEALVDALARLTDLSDGESDGPGHRFPHGRSMDRE*