AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1356140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1356140 MtrunA17_Chr4g0043271 82.400 125 18 2 1 122 118 241 4.67e-64 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1356140 AT2G32080.2 60.000 125 45 3 1 122 121 243 3.42e-41 139
Msa1356140 AT2G32080.1 58.400 125 48 2 1 122 121 244 3.13e-40 137

Find 80 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAAAGATGAATGCTAAAT+TGG 0.149284 tig0015456:-564 Msa1356140:exon
TTGCCAGCTCAGGATGAAAT+TGG 0.220182 tig0015456:-922 Msa1356140:CDS
TGCAGAACAATATGAGAAAA+AGG 0.280604 tig0015456:+429 Msa1356140:intergenic
TGTACAAGGTTAATTTAAAT+AGG 0.283624 tig0015456:-349 Msa1356140:three_prime_UTR
GTTATTTCATTGCAGGTTGC+AGG 0.287453 tig0015456:-721 Msa1356140:intron
GTTGGTGCTGGCTTCATATC+TGG 0.289736 tig0015456:-1000 Msa1356140:CDS
GGTTTCGTCAGGGATATAAA+TGG 0.322628 tig0015456:-506 Msa1356140:three_prime_UTR
AAAATAAACCAAACACTTGT+TGG 0.331676 tig0015456:-394 Msa1356140:three_prime_UTR
CTTGTTGGTCAGCATAATAA+TGG 0.342195 tig0015456:-379 Msa1356140:three_prime_UTR
ATGAAGGGTGGGCAGCATTC+AGG 0.344998 tig0015456:-1231 Msa1356140:CDS
TAGGTAATAACAGAGGAAAA+AGG 0.347485 tig0015456:-330 Msa1356140:three_prime_UTR
CCCTCTGCCAAGGTTTCGTC+AGG 0.351743 tig0015456:-517 Msa1356140:three_prime_UTR
TCAATCATTCTTCCATTGTC+GGG 0.356300 tig0015456:-685 Msa1356140:CDS
TCAAAGATGAATGCTAAATT+GGG 0.365376 tig0015456:-563 Msa1356140:exon
TTGTTCCTGCAGGAAGTTCC+AGG 0.375829 tig0015456:-1255 Msa1356140:CDS
TTGTACTTGATTGGATAGTT+TGG 0.379961 tig0015456:-297 Msa1356140:three_prime_UTR
CTCAATCATTCTTCCATTGT+CGG 0.380080 tig0015456:-686 Msa1356140:CDS
ACCAGAGACTTGTACTTGAT+TGG 0.383457 tig0015456:-306 Msa1356140:three_prime_UTR
GGCAGCATTCAGGAACATTC+TGG 0.414246 tig0015456:-1221 Msa1356140:CDS
TATGCTGACCAACAAGTGTT+TGG 0.414809 tig0015456:+386 Msa1356140:intergenic
TCAGGATGAAATTGGGAACT+TGG 0.414921 tig0015456:-914 Msa1356140:CDS
AAGCAGTTTCATGAGATTGT+TGG 0.420908 tig0015456:-658 Msa1356140:CDS
CATACCCTCAATTCGGTCCT+TGG 0.435320 tig0015456:+618 Msa1356140:intergenic
GTCGGTTGATTTGCCAGCTC+AGG 0.437800 tig0015456:-932 Msa1356140:CDS
TGCTGCCCACCCTTCATCCC+TGG 0.445063 tig0015456:+1237 Msa1356140:intergenic
ATGAAACCTGATTCGGAAGA+AGG 0.447366 tig0015456:+1170 Msa1356140:intergenic
CTTTCAGATGATGTTGGTGC+TGG 0.450565 tig0015456:-1012 Msa1356140:CDS
AATTTAGCATTCATCTTTGA+GGG 0.451848 tig0015456:+566 Msa1356140:intergenic
TGAGATTGTTGGTCACTTTG+TGG 0.454440 tig0015456:-647 Msa1356140:CDS
CAATTTAGCATTCATCTTTG+AGG 0.455509 tig0015456:+565 Msa1356140:intergenic
TGCCAGCTCAGGATGAAATT+GGG 0.464017 tig0015456:-921 Msa1356140:CDS
CAAAAGAATGAAACCTGATT+CGG 0.468159 tig0015456:+1163 Msa1356140:intergenic
CAGGATGAAATTGGGAACTT+GGG 0.472661 tig0015456:-913 Msa1356140:CDS
TTGTTGGTCAGCATAATAAT+GGG 0.477816 tig0015456:-378 Msa1356140:three_prime_UTR
GCAGGAAGTTCCAGGGATGA+AGG 0.480780 tig0015456:-1247 Msa1356140:CDS
TCCAATCAAGTACAAGTCTC+TGG 0.482022 tig0015456:+305 Msa1356140:intergenic
TTGATTGGATAGTTTGGCGA+TGG 0.501050 tig0015456:-291 Msa1356140:three_prime_UTR
TGTCTGAAGCTTCTGTCAGC+AGG 0.503389 tig0015456:-1294 Msa1356140:CDS
TGATTGGATAGTTTGGCGAT+GGG 0.510975 tig0015456:-290 Msa1356140:three_prime_UTR
CGACTACTTTCAGATGATGT+TGG 0.519100 tig0015456:-1018 Msa1356140:CDS
GTTTCGTCAGGGATATAAAT+GGG 0.519846 tig0015456:-505 Msa1356140:three_prime_UTR
TTATATCCCTGACGAAACCT+TGG 0.520016 tig0015456:+510 Msa1356140:intergenic
CAACTGACATACCCTCAATT+CGG 0.522913 tig0015456:+611 Msa1356140:intergenic
TTCCCAATTTCATCCTGAGC+TGG 0.526038 tig0015456:+919 Msa1356140:intergenic
AATCACCAAGGACCGAATTG+AGG 0.544438 tig0015456:-623 Msa1356140:CDS
AGCACTATAATTGTTCCTGC+AGG 0.545453 tig0015456:-1265 Msa1356140:CDS
ACAGCAAAATTCTGAATCCT+CGG 0.546233 tig0015456:-1043 Msa1356140:intron
ATCACCAAGGACCGAATTGA+GGG 0.546646 tig0015456:-622 Msa1356140:CDS
CTTCTGAATTGAATGTGGAC+AGG 0.549374 tig0015456:-954 Msa1356140:CDS
TCATCCCTGGAACTTCCTGC+AGG 0.551352 tig0015456:+1250 Msa1356140:intergenic
AAGTTCCAGGGATGAAGGGT+GGG 0.558834 tig0015456:-1242 Msa1356140:CDS
CCCTGACGAAACCTTGGCAG+AGG 0.559917 tig0015456:+516 Msa1356140:intergenic
CGACAATGGAAGAATGATTG+AGG 0.563454 tig0015456:+687 Msa1356140:intergenic
CACATTCAATTCAGAAGAGG+TGG 0.568175 tig0015456:+960 Msa1356140:intergenic
CAATCATTCTTCCATTGTCG+GGG 0.572962 tig0015456:-684 Msa1356140:CDS
TTCAATTCAGAAGAGGTGGC+AGG 0.574176 tig0015456:+964 Msa1356140:intergenic
CAGGAAGTTCCAGGGATGAA+GGG 0.577060 tig0015456:-1246 Msa1356140:CDS
GAAGTTCCAGGGATGAAGGG+TGG 0.581907 tig0015456:-1243 Msa1356140:CDS
CAGGAACAATTATAGTGCTA+CGG 0.587717 tig0015456:+1269 Msa1356140:intergenic
TGTTCCTGCAGGAAGTTCCA+GGG 0.590512 tig0015456:-1254 Msa1356140:CDS
TTTAGCATTCATCTTTGAGG+GGG 0.591689 tig0015456:+568 Msa1356140:intergenic
AACTGCTTGAGCCCCGACAA+TGG 0.592807 tig0015456:+673 Msa1356140:intergenic
CCTGACGAAACCTTGGCAGA+GGG 0.594509 tig0015456:+517 Msa1356140:intergenic
AGGATGAAATTGGGAACTTG+GGG 0.597453 tig0015456:-912 Msa1356140:CDS
ATAAGTAGTCCCCTCTGCCA+AGG 0.601566 tig0015456:-527 Msa1356140:three_prime_UTR
CACCTCTTCTGAATTGAATG+TGG 0.629994 tig0015456:-959 Msa1356140:CDS
CAAAGATGAATGCTAAATTG+GGG 0.630789 tig0015456:-562 Msa1356140:exon
GTCCACATTCAATTCAGAAG+AGG 0.634204 tig0015456:+957 Msa1356140:intergenic
ATAATGGGAATATATGTACA+AGG 0.644805 tig0015456:-363 Msa1356140:three_prime_UTR
ATTTAAATAGGTAATAACAG+AGG 0.647085 tig0015456:-337 Msa1356140:three_prime_UTR
GATTGGATAGTTTGGCGATG+GGG 0.649419 tig0015456:-289 Msa1356140:three_prime_UTR
ATTTAGCATTCATCTTTGAG+GGG 0.653146 tig0015456:+567 Msa1356140:intergenic
TGAATTGAATGTGGACAGGT+CGG 0.657373 tig0015456:-950 Msa1356140:CDS
GAATGCTAAATTGGGGCTTG+TGG 0.662238 tig0015456:-555 Msa1356140:exon
ATCTGAAAGTAGTCGCTCCG+AGG 0.679752 tig0015456:+1026 Msa1356140:intergenic
CCTCTGCCAAGGTTTCGTCA+GGG 0.688592 tig0015456:-516 Msa1356140:three_prime_UTR
CTGACGAAACCTTGGCAGAG+GGG 0.689780 tig0015456:+518 Msa1356140:intergenic
TCACTTTGTGGAAATCACCA+AGG 0.693256 tig0015456:-635 Msa1356140:CDS
CGGAAGAAGGAAAAGCCTCG+AGG 0.708267 tig0015456:+1183 Msa1356140:intergenic
CAGAGATTAATGAAGCCTCG+AGG 0.735251 tig0015456:-1198 Msa1356140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGATTTTTCTTTGATCT+TGG - tig0015456:670-689 Msa1356140:CDS 20.0%
!!! AAAGATTTTTCTTTGATCTT+GGG - tig0015456:671-690 Msa1356140:CDS 20.0%
!!! AATTTTTGTTATTTCATTGC+AGG - tig0015456:805-824 Msa1356140:intron 20.0%
!! TGTACAAGGTTAATTTAAAT+AGG - tig0015456:1184-1203 Msa1356140:CDS 20.0%
!! ATTTAAATAGGTAATAACAG+AGG - tig0015456:1196-1215 Msa1356140:CDS 20.0%
!!! AAAAGATTTTTCTTTGATCT+TGG - tig0015456:670-689 Msa1356140:CDS 20.0%
!!! AAAGATTTTTCTTTGATCTT+GGG - tig0015456:671-690 Msa1356140:CDS 20.0%
!!! AATTTTTGTTATTTCATTGC+AGG - tig0015456:805-824 Msa1356140:intron 20.0%
!! TGTACAAGGTTAATTTAAAT+AGG - tig0015456:1184-1203 Msa1356140:CDS 20.0%
!! ATTTAAATAGGTAATAACAG+AGG - tig0015456:1196-1215 Msa1356140:CDS 20.0%
!! AAAGATAACTTGGAAAGTTT+TGG + tig0015456:430-449 Msa1356140:intergenic 25.0%
! ACACATCAAAAAAGATAACT+TGG + tig0015456:440-459 Msa1356140:intergenic 25.0%
! GAAGGACAATAAAAACATAA+TGG + tig0015456:741-760 Msa1356140:intergenic 25.0%
! TACAACAAAATAAAGGAAGA+AGG + tig0015456:759-778 Msa1356140:intergenic 25.0%
!!! TCCTTTATTTTGTTGTAGTT+TGG - tig0015456:762-781 Msa1356140:intron 25.0%
!!! CCTTTATTTTGTTGTAGTTT+GGG - tig0015456:763-782 Msa1356140:intron 25.0%
! AATTTAGCATTCATCTTTGA+GGG + tig0015456:970-989 Msa1356140:intergenic 25.0%
! TCAAAGATGAATGCTAAATT+GGG - tig0015456:970-989 Msa1356140:CDS 25.0%
! AAAATAAACCAAACACTTGT+TGG - tig0015456:1139-1158 Msa1356140:intron 25.0%
!! ATAATGGGAATATATGTACA+AGG - tig0015456:1170-1189 Msa1356140:intron 25.0%
!! AAAGATAACTTGGAAAGTTT+TGG + tig0015456:430-449 Msa1356140:intergenic 25.0%
! ACACATCAAAAAAGATAACT+TGG + tig0015456:440-459 Msa1356140:intergenic 25.0%
! GAAGGACAATAAAAACATAA+TGG + tig0015456:741-760 Msa1356140:intergenic 25.0%
! TACAACAAAATAAAGGAAGA+AGG + tig0015456:759-778 Msa1356140:intergenic 25.0%
!!! TCCTTTATTTTGTTGTAGTT+TGG - tig0015456:762-781 Msa1356140:intron 25.0%
!!! CCTTTATTTTGTTGTAGTTT+GGG - tig0015456:763-782 Msa1356140:intron 25.0%
! AATTTAGCATTCATCTTTGA+GGG + tig0015456:970-989 Msa1356140:intergenic 25.0%
! TCAAAGATGAATGCTAAATT+GGG - tig0015456:970-989 Msa1356140:CDS 25.0%
! AAAATAAACCAAACACTTGT+TGG - tig0015456:1139-1158 Msa1356140:intron 25.0%
!! ATAATGGGAATATATGTACA+AGG - tig0015456:1170-1189 Msa1356140:intron 25.0%
CAAAAGAATGAAACCTGATT+CGG + tig0015456:373-392 Msa1356140:intergenic 30.0%
CCTCAGATATTCTTAGAAAA+TGG + tig0015456:711-730 Msa1356140:intergenic 30.0%
!! CCATTTTCTAAGAATATCTG+AGG - tig0015456:708-727 Msa1356140:CDS 30.0%
CCCAAACTACAACAAAATAA+AGG + tig0015456:766-785 Msa1356140:intergenic 30.0%
ATTTAGCATTCATCTTTGAG+GGG + tig0015456:969-988 Msa1356140:intergenic 30.0%
CAATTTAGCATTCATCTTTG+AGG + tig0015456:971-990 Msa1356140:intergenic 30.0%
CTCAAAGATGAATGCTAAAT+TGG - tig0015456:969-988 Msa1356140:CDS 30.0%
CAAAGATGAATGCTAAATTG+GGG - tig0015456:971-990 Msa1356140:CDS 30.0%
ACAATATGAGAAAAAGGAAG+AGG + tig0015456:1101-1120 Msa1356140:intergenic 30.0%
TGCAGAACAATATGAGAAAA+AGG + tig0015456:1107-1126 Msa1356140:intergenic 30.0%
TTGTTGGTCAGCATAATAAT+GGG - tig0015456:1155-1174 Msa1356140:intron 30.0%
TAGGTAATAACAGAGGAAAA+AGG - tig0015456:1203-1222 Msa1356140:CDS 30.0%
AGGTAATAACAGAGGAAAAA+GGG - tig0015456:1204-1223 Msa1356140:CDS 30.0%
TTGTACTTGATTGGATAGTT+TGG - tig0015456:1236-1255 Msa1356140:CDS 30.0%
CAAAAGAATGAAACCTGATT+CGG + tig0015456:373-392 Msa1356140:intergenic 30.0%
CCTCAGATATTCTTAGAAAA+TGG + tig0015456:711-730 Msa1356140:intergenic 30.0%
!! CCATTTTCTAAGAATATCTG+AGG - tig0015456:708-727 Msa1356140:CDS 30.0%
CCCAAACTACAACAAAATAA+AGG + tig0015456:766-785 Msa1356140:intergenic 30.0%
ATTTAGCATTCATCTTTGAG+GGG + tig0015456:969-988 Msa1356140:intergenic 30.0%
CAATTTAGCATTCATCTTTG+AGG + tig0015456:971-990 Msa1356140:intergenic 30.0%
CTCAAAGATGAATGCTAAAT+TGG - tig0015456:969-988 Msa1356140:CDS 30.0%
CAAAGATGAATGCTAAATTG+GGG - tig0015456:971-990 Msa1356140:CDS 30.0%
ACAATATGAGAAAAAGGAAG+AGG + tig0015456:1101-1120 Msa1356140:intergenic 30.0%
TGCAGAACAATATGAGAAAA+AGG + tig0015456:1107-1126 Msa1356140:intergenic 30.0%
TTGTTGGTCAGCATAATAAT+GGG - tig0015456:1155-1174 Msa1356140:intron 30.0%
TAGGTAATAACAGAGGAAAA+AGG - tig0015456:1203-1222 Msa1356140:CDS 30.0%
AGGTAATAACAGAGGAAAAA+GGG - tig0015456:1204-1223 Msa1356140:CDS 30.0%
TTGTACTTGATTGGATAGTT+TGG - tig0015456:1236-1255 Msa1356140:CDS 30.0%
CAGGAACAATTATAGTGCTA+CGG + tig0015456:267-286 Msa1356140:intergenic 35.0%
ACAGCAAAATTCTGAATCCT+CGG - tig0015456:490-509 Msa1356140:three_prime_UTR 35.0%
TTGATCTTGGGAACAACAAT+AGG - tig0015456:683-702 Msa1356140:CDS 35.0%
TGATCTTGGGAACAACAATA+GGG - tig0015456:684-703 Msa1356140:CDS 35.0%
CTCAATCATTCTTCCATTGT+CGG - tig0015456:847-866 Msa1356140:intron 35.0%
TCAATCATTCTTCCATTGTC+GGG - tig0015456:848-867 Msa1356140:intron 35.0%
AAGCAGTTTCATGAGATTGT+TGG - tig0015456:875-894 Msa1356140:intron 35.0%
! TTTAGCATTCATCTTTGAGG+GGG + tig0015456:968-987 Msa1356140:intergenic 35.0%
GTTTCGTCAGGGATATAAAT+GGG - tig0015456:1028-1047 Msa1356140:CDS 35.0%
CTTGTTGGTCAGCATAATAA+TGG - tig0015456:1154-1173 Msa1356140:intron 35.0%
CAGGAACAATTATAGTGCTA+CGG + tig0015456:267-286 Msa1356140:intergenic 35.0%
ACAGCAAAATTCTGAATCCT+CGG - tig0015456:490-509 Msa1356140:three_prime_UTR 35.0%
TTGATCTTGGGAACAACAAT+AGG - tig0015456:683-702 Msa1356140:CDS 35.0%
TGATCTTGGGAACAACAATA+GGG - tig0015456:684-703 Msa1356140:CDS 35.0%
CTCAATCATTCTTCCATTGT+CGG - tig0015456:847-866 Msa1356140:intron 35.0%
TCAATCATTCTTCCATTGTC+GGG - tig0015456:848-867 Msa1356140:intron 35.0%
AAGCAGTTTCATGAGATTGT+TGG - tig0015456:875-894 Msa1356140:intron 35.0%
! TTTAGCATTCATCTTTGAGG+GGG + tig0015456:968-987 Msa1356140:intergenic 35.0%
GTTTCGTCAGGGATATAAAT+GGG - tig0015456:1028-1047 Msa1356140:CDS 35.0%
CTTGTTGGTCAGCATAATAA+TGG - tig0015456:1154-1173 Msa1356140:intron 35.0%
AGCACTATAATTGTTCCTGC+AGG - tig0015456:268-287 Msa1356140:three_prime_UTR 40.0%
!! ATGAAACCTGATTCGGAAGA+AGG + tig0015456:366-385 Msa1356140:intergenic 40.0%
!!! AGTTTTTTTTCCCCCTGCTT+AGG - tig0015456:402-421 Msa1356140:three_prime_UTR 40.0%
CGACTACTTTCAGATGATGT+TGG - tig0015456:515-534 Msa1356140:three_prime_UTR 40.0%
CACATTCAATTCAGAAGAGG+TGG + tig0015456:576-595 Msa1356140:intergenic 40.0%
CACCTCTTCTGAATTGAATG+TGG - tig0015456:574-593 Msa1356140:CDS 40.0%
GTCCACATTCAATTCAGAAG+AGG + tig0015456:579-598 Msa1356140:intergenic 40.0%
CTTCTGAATTGAATGTGGAC+AGG - tig0015456:579-598 Msa1356140:CDS 40.0%
TGAATTGAATGTGGACAGGT+CGG - tig0015456:583-602 Msa1356140:CDS 40.0%
TCAGGATGAAATTGGGAACT+TGG - tig0015456:619-638 Msa1356140:CDS 40.0%
CAGGATGAAATTGGGAACTT+GGG - tig0015456:620-639 Msa1356140:CDS 40.0%
AGGATGAAATTGGGAACTTG+GGG - tig0015456:621-640 Msa1356140:CDS 40.0%
! TTGATCAGCTCTGATCACTT+TGG + tig0015456:650-669 Msa1356140:intergenic 40.0%
GATCTTGGGAACAACAATAG+GGG - tig0015456:685-704 Msa1356140:CDS 40.0%
! GTTATTTCATTGCAGGTTGC+AGG - tig0015456:812-831 Msa1356140:intron 40.0%
CGACAATGGAAGAATGATTG+AGG + tig0015456:849-868 Msa1356140:intergenic 40.0%
CAATCATTCTTCCATTGTCG+GGG - tig0015456:849-868 Msa1356140:intron 40.0%
! TGAGATTGTTGGTCACTTTG+TGG - tig0015456:886-905 Msa1356140:intron 40.0%
TCACTTTGTGGAAATCACCA+AGG - tig0015456:898-917 Msa1356140:intron 40.0%
CAACTGACATACCCTCAATT+CGG + tig0015456:925-944 Msa1356140:intergenic 40.0%
TTATATCCCTGACGAAACCT+TGG + tig0015456:1026-1045 Msa1356140:intergenic 40.0%
GGTTTCGTCAGGGATATAAA+TGG - tig0015456:1027-1046 Msa1356140:CDS 40.0%
TATGCTGACCAACAAGTGTT+TGG + tig0015456:1150-1169 Msa1356140:intergenic 40.0%
ACCAGAGACTTGTACTTGAT+TGG - tig0015456:1227-1246 Msa1356140:CDS 40.0%
TCCAATCAAGTACAAGTCTC+TGG + tig0015456:1231-1250 Msa1356140:intergenic 40.0%
TTGATTGGATAGTTTGGCGA+TGG - tig0015456:1242-1261 Msa1356140:CDS 40.0%
TGATTGGATAGTTTGGCGAT+GGG - tig0015456:1243-1262 Msa1356140:CDS 40.0%
AGCACTATAATTGTTCCTGC+AGG - tig0015456:268-287 Msa1356140:three_prime_UTR 40.0%
!! ATGAAACCTGATTCGGAAGA+AGG + tig0015456:366-385 Msa1356140:intergenic 40.0%
!!! AGTTTTTTTTCCCCCTGCTT+AGG - tig0015456:402-421 Msa1356140:three_prime_UTR 40.0%
CGACTACTTTCAGATGATGT+TGG - tig0015456:515-534 Msa1356140:three_prime_UTR 40.0%
CACATTCAATTCAGAAGAGG+TGG + tig0015456:576-595 Msa1356140:intergenic 40.0%
CACCTCTTCTGAATTGAATG+TGG - tig0015456:574-593 Msa1356140:CDS 40.0%
GTCCACATTCAATTCAGAAG+AGG + tig0015456:579-598 Msa1356140:intergenic 40.0%
CTTCTGAATTGAATGTGGAC+AGG - tig0015456:579-598 Msa1356140:CDS 40.0%
TGAATTGAATGTGGACAGGT+CGG - tig0015456:583-602 Msa1356140:CDS 40.0%
TCAGGATGAAATTGGGAACT+TGG - tig0015456:619-638 Msa1356140:CDS 40.0%
CAGGATGAAATTGGGAACTT+GGG - tig0015456:620-639 Msa1356140:CDS 40.0%
AGGATGAAATTGGGAACTTG+GGG - tig0015456:621-640 Msa1356140:CDS 40.0%
! TTGATCAGCTCTGATCACTT+TGG + tig0015456:650-669 Msa1356140:intergenic 40.0%
GATCTTGGGAACAACAATAG+GGG - tig0015456:685-704 Msa1356140:CDS 40.0%
! GTTATTTCATTGCAGGTTGC+AGG - tig0015456:812-831 Msa1356140:intron 40.0%
CGACAATGGAAGAATGATTG+AGG + tig0015456:849-868 Msa1356140:intergenic 40.0%
CAATCATTCTTCCATTGTCG+GGG - tig0015456:849-868 Msa1356140:intron 40.0%
! TGAGATTGTTGGTCACTTTG+TGG - tig0015456:886-905 Msa1356140:intron 40.0%
TCACTTTGTGGAAATCACCA+AGG - tig0015456:898-917 Msa1356140:intron 40.0%
CAACTGACATACCCTCAATT+CGG + tig0015456:925-944 Msa1356140:intergenic 40.0%
TTATATCCCTGACGAAACCT+TGG + tig0015456:1026-1045 Msa1356140:intergenic 40.0%
GGTTTCGTCAGGGATATAAA+TGG - tig0015456:1027-1046 Msa1356140:CDS 40.0%
TATGCTGACCAACAAGTGTT+TGG + tig0015456:1150-1169 Msa1356140:intergenic 40.0%
ACCAGAGACTTGTACTTGAT+TGG - tig0015456:1227-1246 Msa1356140:CDS 40.0%
TCCAATCAAGTACAAGTCTC+TGG + tig0015456:1231-1250 Msa1356140:intergenic 40.0%
TTGATTGGATAGTTTGGCGA+TGG - tig0015456:1242-1261 Msa1356140:CDS 40.0%
TGATTGGATAGTTTGGCGAT+GGG - tig0015456:1243-1262 Msa1356140:CDS 40.0%
CAGAGATTAATGAAGCCTCG+AGG - tig0015456:335-354 Msa1356140:three_prime_UTR 45.0%
! GCTTTTCCTTCTTCCGAATC+AGG - tig0015456:357-376 Msa1356140:three_prime_UTR 45.0%
!! AAAGTTTTGGCGCCTAAGCA+GGG + tig0015456:417-436 Msa1356140:intergenic 45.0%
CTTTCAGATGATGTTGGTGC+TGG - tig0015456:521-540 Msa1356140:three_prime_UTR 45.0%
TTCAATTCAGAAGAGGTGGC+AGG + tig0015456:572-591 Msa1356140:intergenic 45.0%
TTGCCAGCTCAGGATGAAAT+TGG - tig0015456:611-630 Msa1356140:CDS 45.0%
TGCCAGCTCAGGATGAAATT+GGG - tig0015456:612-631 Msa1356140:CDS 45.0%
TTCCCAATTTCATCCTGAGC+TGG + tig0015456:617-636 Msa1356140:intergenic 45.0%
AATCACCAAGGACCGAATTG+AGG - tig0015456:910-929 Msa1356140:CDS 45.0%
ATCACCAAGGACCGAATTGA+GGG - tig0015456:911-930 Msa1356140:CDS 45.0%
TTTGAGGGGGATCAATTGTG+CGG + tig0015456:955-974 Msa1356140:intergenic 45.0%
GAATGCTAAATTGGGGCTTG+TGG - tig0015456:978-997 Msa1356140:CDS 45.0%
GATTGGATAGTTTGGCGATG+GGG - tig0015456:1244-1263 Msa1356140:CDS 45.0%
CAGAGATTAATGAAGCCTCG+AGG - tig0015456:335-354 Msa1356140:three_prime_UTR 45.0%
! GCTTTTCCTTCTTCCGAATC+AGG - tig0015456:357-376 Msa1356140:three_prime_UTR 45.0%
!! AAAGTTTTGGCGCCTAAGCA+GGG + tig0015456:417-436 Msa1356140:intergenic 45.0%
CTTTCAGATGATGTTGGTGC+TGG - tig0015456:521-540 Msa1356140:three_prime_UTR 45.0%
TTCAATTCAGAAGAGGTGGC+AGG + tig0015456:572-591 Msa1356140:intergenic 45.0%
TTGCCAGCTCAGGATGAAAT+TGG - tig0015456:611-630 Msa1356140:CDS 45.0%
TGCCAGCTCAGGATGAAATT+GGG - tig0015456:612-631 Msa1356140:CDS 45.0%
TTCCCAATTTCATCCTGAGC+TGG + tig0015456:617-636 Msa1356140:intergenic 45.0%
AATCACCAAGGACCGAATTG+AGG - tig0015456:910-929 Msa1356140:CDS 45.0%
ATCACCAAGGACCGAATTGA+GGG - tig0015456:911-930 Msa1356140:CDS 45.0%
TTTGAGGGGGATCAATTGTG+CGG + tig0015456:955-974 Msa1356140:intergenic 45.0%
GAATGCTAAATTGGGGCTTG+TGG - tig0015456:978-997 Msa1356140:CDS 45.0%
GATTGGATAGTTTGGCGATG+GGG - tig0015456:1244-1263 Msa1356140:CDS 45.0%
! TGTCTGAAGCTTCTGTCAGC+AGG - tig0015456:239-258 Msa1356140:three_prime_UTR 50.0%
TTGTTCCTGCAGGAAGTTCC+AGG - tig0015456:278-297 Msa1356140:three_prime_UTR 50.0%
TGTTCCTGCAGGAAGTTCCA+GGG - tig0015456:279-298 Msa1356140:three_prime_UTR 50.0%
CAGGAAGTTCCAGGGATGAA+GGG - tig0015456:287-306 Msa1356140:three_prime_UTR 50.0%
AAGTTCCAGGGATGAAGGGT+GGG - tig0015456:291-310 Msa1356140:three_prime_UTR 50.0%
GGCAGCATTCAGGAACATTC+TGG - tig0015456:312-331 Msa1356140:three_prime_UTR 50.0%
!! AAGTTTTGGCGCCTAAGCAG+GGG + tig0015456:416-435 Msa1356140:intergenic 50.0%
!! GAAAGTTTTGGCGCCTAAGC+AGG + tig0015456:418-437 Msa1356140:intergenic 50.0%
ATCTGAAAGTAGTCGCTCCG+AGG + tig0015456:510-529 Msa1356140:intergenic 50.0%
!! GTTGGTGCTGGCTTCATATC+TGG - tig0015456:533-552 Msa1356140:three_prime_UTR 50.0%
CATACCCTCAATTCGGTCCT+TGG + tig0015456:918-937 Msa1356140:intergenic 50.0%
ATAAGTAGTCCCCTCTGCCA+AGG - tig0015456:1006-1025 Msa1356140:CDS 50.0%
! TGTCTGAAGCTTCTGTCAGC+AGG - tig0015456:239-258 Msa1356140:three_prime_UTR 50.0%
TTGTTCCTGCAGGAAGTTCC+AGG - tig0015456:278-297 Msa1356140:three_prime_UTR 50.0%
TGTTCCTGCAGGAAGTTCCA+GGG - tig0015456:279-298 Msa1356140:three_prime_UTR 50.0%
CAGGAAGTTCCAGGGATGAA+GGG - tig0015456:287-306 Msa1356140:three_prime_UTR 50.0%
AAGTTCCAGGGATGAAGGGT+GGG - tig0015456:291-310 Msa1356140:three_prime_UTR 50.0%
GGCAGCATTCAGGAACATTC+TGG - tig0015456:312-331 Msa1356140:three_prime_UTR 50.0%
!! AAGTTTTGGCGCCTAAGCAG+GGG + tig0015456:416-435 Msa1356140:intergenic 50.0%
!! GAAAGTTTTGGCGCCTAAGC+AGG + tig0015456:418-437 Msa1356140:intergenic 50.0%
ATCTGAAAGTAGTCGCTCCG+AGG + tig0015456:510-529 Msa1356140:intergenic 50.0%
!! GTTGGTGCTGGCTTCATATC+TGG - tig0015456:533-552 Msa1356140:three_prime_UTR 50.0%
CATACCCTCAATTCGGTCCT+TGG + tig0015456:918-937 Msa1356140:intergenic 50.0%
ATAAGTAGTCCCCTCTGCCA+AGG - tig0015456:1006-1025 Msa1356140:CDS 50.0%
TCATCCCTGGAACTTCCTGC+AGG + tig0015456:286-305 Msa1356140:intergenic 55.0%
GCAGGAAGTTCCAGGGATGA+AGG - tig0015456:286-305 Msa1356140:three_prime_UTR 55.0%
GAAGTTCCAGGGATGAAGGG+TGG - tig0015456:290-309 Msa1356140:three_prime_UTR 55.0%
ATGAAGGGTGGGCAGCATTC+AGG - tig0015456:302-321 Msa1356140:three_prime_UTR 55.0%
CGGAAGAAGGAAAAGCCTCG+AGG + tig0015456:353-372 Msa1356140:intergenic 55.0%
!! AGTTTTGGCGCCTAAGCAGG+GGG + tig0015456:415-434 Msa1356140:intergenic 55.0%
!! GTCGGTTGATTTGCCAGCTC+AGG - tig0015456:601-620 Msa1356140:CDS 55.0%
AACTGCTTGAGCCCCGACAA+TGG + tig0015456:863-882 Msa1356140:intergenic 55.0%
CTGACGAAACCTTGGCAGAG+GGG + tig0015456:1018-1037 Msa1356140:intergenic 55.0%
CCTGACGAAACCTTGGCAGA+GGG + tig0015456:1019-1038 Msa1356140:intergenic 55.0%
CCTCTGCCAAGGTTTCGTCA+GGG - tig0015456:1017-1036 Msa1356140:CDS 55.0%
TCATCCCTGGAACTTCCTGC+AGG + tig0015456:286-305 Msa1356140:intergenic 55.0%
GCAGGAAGTTCCAGGGATGA+AGG - tig0015456:286-305 Msa1356140:three_prime_UTR 55.0%
GAAGTTCCAGGGATGAAGGG+TGG - tig0015456:290-309 Msa1356140:three_prime_UTR 55.0%
ATGAAGGGTGGGCAGCATTC+AGG - tig0015456:302-321 Msa1356140:three_prime_UTR 55.0%
CGGAAGAAGGAAAAGCCTCG+AGG + tig0015456:353-372 Msa1356140:intergenic 55.0%
!! AGTTTTGGCGCCTAAGCAGG+GGG + tig0015456:415-434 Msa1356140:intergenic 55.0%
!! GTCGGTTGATTTGCCAGCTC+AGG - tig0015456:601-620 Msa1356140:CDS 55.0%
AACTGCTTGAGCCCCGACAA+TGG + tig0015456:863-882 Msa1356140:intergenic 55.0%
CTGACGAAACCTTGGCAGAG+GGG + tig0015456:1018-1037 Msa1356140:intergenic 55.0%
CCTGACGAAACCTTGGCAGA+GGG + tig0015456:1019-1038 Msa1356140:intergenic 55.0%
CCTCTGCCAAGGTTTCGTCA+GGG - tig0015456:1017-1036 Msa1356140:CDS 55.0%
CCCTCTGCCAAGGTTTCGTC+AGG - tig0015456:1016-1035 Msa1356140:CDS 60.0%
CCCTGACGAAACCTTGGCAG+AGG + tig0015456:1020-1039 Msa1356140:intergenic 60.0%
CCCTCTGCCAAGGTTTCGTC+AGG - tig0015456:1016-1035 Msa1356140:CDS 60.0%
CCCTGACGAAACCTTGGCAG+AGG + tig0015456:1020-1039 Msa1356140:intergenic 60.0%
TGCTGCCCACCCTTCATCCC+TGG + tig0015456:299-318 Msa1356140:intergenic 65.0%
TGCTGCCCACCCTTCATCCC+TGG + tig0015456:299-318 Msa1356140:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0015456 gene 238 1317 238 ID=Msa1356140;Name=Msa1356140
tig0015456 mRNA 238 1317 238 ID=Msa1356140-mRNA-1;Parent=Msa1356140;Name=Msa1356140-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0.5|0.33|1|1|1|3|331|152
tig0015456 exon 238 728 238 ID=Msa1356140-mRNA-1:exon:9767;Parent=Msa1356140-mRNA-1
tig0015456 exon 904 1061 904 ID=Msa1356140-mRNA-1:exon:9766;Parent=Msa1356140-mRNA-1
tig0015456 exon 1177 1317 1177 ID=Msa1356140-mRNA-1:exon:9765;Parent=Msa1356140-mRNA-1
tig0015456 CDS 1177 1317 1177 ID=Msa1356140-mRNA-1:cds;Parent=Msa1356140-mRNA-1
tig0015456 CDS 904 1061 904 ID=Msa1356140-mRNA-1:cds;Parent=Msa1356140-mRNA-1
tig0015456 CDS 569 728 569 ID=Msa1356140-mRNA-1:cds;Parent=Msa1356140-mRNA-1
tig0015456 three_prime_UTR 238 568 238 ID=Msa1356140-mRNA-1:three_prime_utr;Parent=Msa1356140-mRNA-1
Gene Sequence

>Msa1356140

GTGTCTGAAGCTTCTGTCAGCAGGAACCGTAGCACTATAATTGTTCCTGCAGGAAGTTCCAGGGATGAAGGGTGGGCAGCATTCAGGAACATTCTGGCAGAGATTAATGAAGCCTCGAGGCTTTTCCTTCTTCCGAATCAGCAAAATTCTGAATCCTCGGAGCGACTACTTTCAGATGATGTTGGTGCTGGCTTCATATCTGGTCATAATACTACACCTGCCACCTCTTCTGAATTGAATGTGGACAGGTCGGTTGATTTGCCAGCTCAGGATGAAATTGGGAACTTGGGGTCTCCAAAGTTGCAGGTTCTGATCGATCCTCAATCATTCTTCCATTGTCGGGGCTCAAGCAGTTTCATGAGATTGTTGGTCACTTTGTGGAAATCACCAAGGACCGAATTGAGGGTATGTCAGTTGCTAATGTCCGCACAATTGATCCCCCTCAAAGATGAATGCTAA
Protein sequence

>Msa1356140

VSEASVSRNRSTIIVPAGSSRDEGWAAFRNILAEINEASRLFLLPNQQNSESSERLLSDDVGAGFISGHNTTPATSSELNVDRSVDLPAQDEIGNLGSPKLQVLIDPQSFFHCRGSSSFMRLLVTLWKSPRTELRVCQLLMSAQLIPLKDEC*