Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0833900 | AES94328.1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.26e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0833900 | G7JXH1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 6.02e-32 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0071250 | Msa0833900 | 0.812972 | 3.239483e-51 | -8.615850e-47 |
| Msa0611540 | Msa0833900 | -0.805206 | 1.492895e-49 | -8.615850e-47 |
| Msa0679030 | Msa0833900 | 0.807438 | 5.054348e-50 | -8.615850e-47 |
| Msa0754820 | Msa0833900 | 0.879775 | 9.462377e-70 | -8.615850e-47 |
| Msa0796620 | Msa0833900 | 0.964134 | 6.027565e-123 | -8.615850e-47 |
| Msa0833900 | Msa0834170 | 0.957987 | 7.145791e-116 | -8.615850e-47 |
| Msa0833900 | Msa1388080 | 0.854946 | 8.771578e-62 | -8.615850e-47 |
| Msa0144530 | Msa0833900 | 0.809346 | 1.980354e-50 | -8.615850e-47 |
| Msa0148080 | Msa0833900 | 0.800369 | 1.488943e-48 | -8.615850e-47 |
| Msa0204960 | Msa0833900 | 0.806244 | 9.038387e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0833900 | MtrunA17_Chr5g0399651 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.16e-35 | 114 |
| Msa0833900 | MtrunA17_Chr1g0196811 | 55.556 | 45 | 20 | 0 | 9 | 53 | 37 | 81 | 2.87e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0833900 | AT2G35658.3 | 53.571 | 56 | 25 | 1 | 2 | 56 | 33 | 88 | 7.06e-18 | 70.5 |
| Msa0833900 | AT2G35658.1 | 52.830 | 53 | 24 | 1 | 2 | 53 | 33 | 85 | 1.79e-16 | 66.6 |
| Msa0833900 | AT2G35658.2 | 50.909 | 55 | 26 | 1 | 2 | 55 | 33 | 87 | 1.82e-16 | 67.0 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAAGATGAACAGCTTTC+TGG | 0.180591 | 5_4:-7008380 | None:intergenic |
| TTTCATTATATTAGATTGTT+TGG | 0.314457 | 5_4:+7008630 | Msa0833900:three_prime_UTR |
| CTAAATTAGTTGGAGTGGTT+TGG | 0.336990 | 5_4:+7008222 | Msa0833900:five_prime_UTR |
| TTCTTTGTGCTATCATCCTC+TGG | 0.356172 | 5_4:+7008415 | Msa0833900:CDS |
| GTTCTTCTCTTCCCCAACTA+AGG | 0.380504 | 5_4:+7008437 | Msa0833900:CDS |
| GAATTTGTGTATCAATAGAT+TGG | 0.395682 | 5_4:+7008743 | Msa0833900:three_prime_UTR |
| GATAAGATGAACAGCTTTCT+GGG | 0.396373 | 5_4:-7008379 | None:intergenic |
| AACAGAAGAGAACCTTAGTT+GGG | 0.415115 | 5_4:-7008449 | None:intergenic |
| TATATCACTGGAATCTAGAT+AGG | 0.428049 | 5_4:+7008721 | Msa0833900:three_prime_UTR |
| TCTCTCCCATGTTGCAAAGA+AGG | 0.432445 | 5_4:+7008335 | Msa0833900:CDS |
| ATGCATAGTTTCAGCATAAG+AGG | 0.446059 | 5_4:+7008570 | Msa0833900:three_prime_UTR |
| CTCCAACTAATTTAGTTCGC+TGG | 0.460222 | 5_4:-7008214 | None:intergenic |
| AGCCAGCGAACTAAATTAGT+TGG | 0.462152 | 5_4:+7008212 | Msa0833900:five_prime_UTR |
| ATTTGTACCAACTATATCAC+TGG | 0.477992 | 5_4:+7008709 | Msa0833900:three_prime_UTR |
| AAACAGAAGAGAACCTTAGT+TGG | 0.485222 | 5_4:-7008450 | None:intergenic |
| GAGGATCGTATGCTGGTAGT+AGG | 0.508758 | 5_4:-7008314 | None:intergenic |
| CTAGATTCCAGTGATATAGT+TGG | 0.510649 | 5_4:-7008716 | None:intergenic |
| CAGATTACATAATTTGATGA+CGG | 0.538839 | 5_4:+7008547 | Msa0833900:intron |
| GCACAAAGAAGAACAAGAAG+AGG | 0.551389 | 5_4:-7008402 | None:intergenic |
| TTCTTTGCAACATGGGAGAG+AGG | 0.554290 | 5_4:-7008333 | None:intergenic |
| CAGTTCCTTCTTTGCAACAT+GGG | 0.554330 | 5_4:-7008340 | None:intergenic |
| TGGGAGAGAGGATCGTATGC+TGG | 0.560198 | 5_4:-7008321 | None:intergenic |
| GCGAACTAAATTAGTTGGAG+TGG | 0.562737 | 5_4:+7008217 | Msa0833900:five_prime_UTR |
| ACAGTTCCTTCTTTGCAACA+TGG | 0.577429 | 5_4:-7008341 | None:intergenic |
| CACAAAGAAGAACAAGAAGA+GGG | 0.602024 | 5_4:-7008401 | None:intergenic |
| TTGGGGAAGAGAAGAACCAG+AGG | 0.622299 | 5_4:-7008431 | None:intergenic |
| ACAGAAGAGAACCTTAGTTG+GGG | 0.648692 | 5_4:-7008448 | None:intergenic |
| TAGAAACAAACAAACAAACA+TGG | 0.652612 | 5_4:+7008275 | Msa0833900:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTATATTAGATTGTT+TGG | + | chr5_4:7008630-7008649 | Msa0833900:three_prime_UTR | 15.0% |
| !! | AAGTTATCATACAATAAAAA+TGG | - | chr5_4:7008800-7008819 | None:intergenic | 15.0% |
| !!! | TTCAATTTTCAGTTTTAGAT+TGG | + | chr5_4:7008474-7008493 | Msa0833900:intron | 20.0% |
| !! | ATTAAAGTAGTTAGCAAATA+TGG | - | chr5_4:7008510-7008529 | None:intergenic | 20.0% |
| !! | ACTATTTCGTGTTTTTCTTT+TGG | - | chr5_4:7008176-7008195 | None:intergenic | 25.0% |
| ! | TAGAAACAAACAAACAAACA+TGG | + | chr5_4:7008275-7008294 | Msa0833900:exon | 25.0% |
| ! | CAGATTACATAATTTGATGA+CGG | + | chr5_4:7008547-7008566 | Msa0833900:intron | 25.0% |
| ! | GAATTTGTGTATCAATAGAT+TGG | + | chr5_4:7008743-7008762 | Msa0833900:three_prime_UTR | 25.0% |
| ATTTGTACCAACTATATCAC+TGG | + | chr5_4:7008709-7008728 | Msa0833900:three_prime_UTR | 30.0% | |
| TATATCACTGGAATCTAGAT+AGG | + | chr5_4:7008721-7008740 | Msa0833900:three_prime_UTR | 30.0% | |
| CTAAATTAGTTGGAGTGGTT+TGG | + | chr5_4:7008222-7008241 | Msa0833900:five_prime_UTR | 35.0% | |
| GATAAGATGAACAGCTTTCT+GGG | - | chr5_4:7008382-7008401 | None:intergenic | 35.0% | |
| CACAAAGAAGAACAAGAAGA+GGG | - | chr5_4:7008404-7008423 | None:intergenic | 35.0% | |
| AACAGAAGAGAACCTTAGTT+GGG | - | chr5_4:7008452-7008471 | None:intergenic | 35.0% | |
| AAACAGAAGAGAACCTTAGT+TGG | - | chr5_4:7008453-7008472 | None:intergenic | 35.0% | |
| ATGCATAGTTTCAGCATAAG+AGG | + | chr5_4:7008570-7008589 | Msa0833900:three_prime_UTR | 35.0% | |
| CTAGATTCCAGTGATATAGT+TGG | - | chr5_4:7008719-7008738 | None:intergenic | 35.0% | |
| AGCCAGCGAACTAAATTAGT+TGG | + | chr5_4:7008212-7008231 | Msa0833900:five_prime_UTR | 40.0% | |
| ! | CTCCAACTAATTTAGTTCGC+TGG | - | chr5_4:7008217-7008236 | None:intergenic | 40.0% |
| GCGAACTAAATTAGTTGGAG+TGG | + | chr5_4:7008217-7008236 | Msa0833900:five_prime_UTR | 40.0% | |
| CAGTTCCTTCTTTGCAACAT+GGG | - | chr5_4:7008343-7008362 | None:intergenic | 40.0% | |
| ACAGTTCCTTCTTTGCAACA+TGG | - | chr5_4:7008344-7008363 | None:intergenic | 40.0% | |
| GGATAAGATGAACAGCTTTC+TGG | - | chr5_4:7008383-7008402 | None:intergenic | 40.0% | |
| GCACAAAGAAGAACAAGAAG+AGG | - | chr5_4:7008405-7008424 | None:intergenic | 40.0% | |
| TTCTTTGTGCTATCATCCTC+TGG | + | chr5_4:7008415-7008434 | Msa0833900:CDS | 40.0% | |
| ACAGAAGAGAACCTTAGTTG+GGG | - | chr5_4:7008451-7008470 | None:intergenic | 40.0% | |
| TTCTTTGCAACATGGGAGAG+AGG | - | chr5_4:7008336-7008355 | None:intergenic | 45.0% | |
| TCTCTCCCATGTTGCAAAGA+AGG | + | chr5_4:7008335-7008354 | Msa0833900:CDS | 45.0% | |
| GTTCTTCTCTTCCCCAACTA+AGG | + | chr5_4:7008437-7008456 | Msa0833900:CDS | 45.0% | |
| GAGGATCGTATGCTGGTAGT+AGG | - | chr5_4:7008317-7008336 | None:intergenic | 50.0% | |
| TTGGGGAAGAGAAGAACCAG+AGG | - | chr5_4:7008434-7008453 | None:intergenic | 50.0% | |
| TGGGAGAGAGGATCGTATGC+TGG | - | chr5_4:7008324-7008343 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 7008166 | 7008860 | 7008166 | ID=Msa0833900;Name=Msa0833900 |
| chr5_4 | mRNA | 7008166 | 7008860 | 7008166 | ID=Msa0833900-mRNA-1;Parent=Msa0833900;Name=Msa0833900-mRNA-1;_AED=0.00;_eAED=0.00;_QI=128|1|1|1|1|1|2|302|57 |
| chr5_4 | exon | 7008166 | 7008458 | 7008166 | ID=Msa0833900-mRNA-1:exon:3073;Parent=Msa0833900-mRNA-1 |
| chr5_4 | exon | 7008550 | 7008860 | 7008550 | ID=Msa0833900-mRNA-1:exon:3074;Parent=Msa0833900-mRNA-1 |
| chr5_4 | five_prime_UTR | 7008166 | 7008293 | 7008166 | ID=Msa0833900-mRNA-1:five_prime_utr;Parent=Msa0833900-mRNA-1 |
| chr5_4 | CDS | 7008294 | 7008458 | 7008294 | ID=Msa0833900-mRNA-1:cds;Parent=Msa0833900-mRNA-1 |
| chr5_4 | CDS | 7008550 | 7008558 | 7008550 | ID=Msa0833900-mRNA-1:cds;Parent=Msa0833900-mRNA-1 |
| chr5_4 | three_prime_UTR | 7008559 | 7008860 | 7008559 | ID=Msa0833900-mRNA-1:three_prime_utr;Parent=Msa0833900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
