Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0796620 | AES94328.1 | 98.246 | 57 | 1 | 0 | 11 | 67 | 1 | 57 | 4.27e-32 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0796620 | G7JXH1 | 98.246 | 57 | 1 | 0 | 11 | 67 | 1 | 57 | 2.04e-32 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0754820 | Msa0796620 | 0.850487 | 1.644722e-60 | -8.615850e-47 |
| Msa0796620 | Msa0833900 | 0.964134 | 6.027565e-123 | -8.615850e-47 |
| Msa0796620 | Msa0834170 | 0.952847 | 9.989519e-111 | -8.615850e-47 |
| Msa0796620 | Msa1341060 | 0.802318 | 5.938208e-49 | -8.615850e-47 |
| Msa0796620 | Msa1388080 | 0.828976 | 6.663753e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0796620 | MtrunA17_Chr5g0399651 | 98.246 | 57 | 1 | 0 | 11 | 67 | 1 | 57 | 3.93e-36 | 115 |
| Msa0796620 | MtrunA17_Chr1g0196811 | 55.556 | 45 | 20 | 0 | 19 | 63 | 37 | 81 | 2.15e-14 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0796620 | AT2G35658.3 | 51.667 | 60 | 28 | 1 | 8 | 66 | 29 | 88 | 1.51e-18 | 72.4 |
| Msa0796620 | AT2G35658.1 | 50.000 | 58 | 28 | 1 | 8 | 64 | 29 | 86 | 3.96e-17 | 68.9 |
| Msa0796620 | AT2G35658.2 | 50.877 | 57 | 27 | 1 | 8 | 63 | 29 | 85 | 4.87e-17 | 68.9 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAAGATGAACAGCTTTC+TGG | 0.180591 | 5_3:+8657383 | None:intergenic |
| TTTCATTATATTAGATTGTT+TGG | 0.314457 | 5_3:-8657133 | Msa0796620:three_prime_UTR |
| CTAAATTAGTTGGAGTGGTT+TGG | 0.336990 | 5_3:-8657540 | Msa0796620:five_prime_UTR |
| TTCTTTGTGCTATCATCCTC+TGG | 0.356172 | 5_3:-8657348 | Msa0796620:CDS |
| GTTCTTCTCTTCCCCAACTA+AGG | 0.380504 | 5_3:-8657326 | Msa0796620:intron |
| GAATTTGTGTATCAATAGAT+TGG | 0.395682 | 5_3:-8657020 | Msa0796620:three_prime_UTR |
| GATAAGATGAACAGCTTTCT+GGG | 0.396373 | 5_3:+8657384 | None:intergenic |
| AACAGAAGAGAACCTTAGTT+GGG | 0.415115 | 5_3:+8657314 | None:intergenic |
| TATATCACTGGAATCTAGAT+AGG | 0.428049 | 5_3:-8657042 | Msa0796620:three_prime_UTR |
| TCTCTCCCATGTTGCAAAGA+AGG | 0.432445 | 5_3:-8657428 | Msa0796620:CDS |
| ATGCATAGTTTCAGCATAAG+AGG | 0.446059 | 5_3:-8657193 | Msa0796620:three_prime_UTR |
| CTCCAACTAATTTAGTTCGC+TGG | 0.460222 | 5_3:+8657548 | None:intergenic |
| AGCCAGCGAACTAAATTAGT+TGG | 0.462152 | 5_3:-8657550 | Msa0796620:five_prime_UTR |
| ATTTGTACCAACTATATCAC+TGG | 0.477992 | 5_3:-8657054 | Msa0796620:three_prime_UTR |
| AAACAGAAGAGAACCTTAGT+TGG | 0.485222 | 5_3:+8657313 | None:intergenic |
| GAGGATCGTATGCTGGTAGT+AGG | 0.508758 | 5_3:+8657449 | None:intergenic |
| CTAGATTCCAGTGATATAGT+TGG | 0.510649 | 5_3:+8657047 | None:intergenic |
| CAGATTACATAATTTGATGA+CGG | 0.538839 | 5_3:-8657216 | Msa0796620:exon |
| GCACAAAGAAGAACAAGAAG+AGG | 0.551389 | 5_3:+8657361 | None:intergenic |
| TTCTTTGCAACATGGGAGAG+AGG | 0.554290 | 5_3:+8657430 | None:intergenic |
| CAGTTCCTTCTTTGCAACAT+GGG | 0.554330 | 5_3:+8657423 | None:intergenic |
| TGGGAGAGAGGATCGTATGC+TGG | 0.560198 | 5_3:+8657442 | None:intergenic |
| GCGAACTAAATTAGTTGGAG+TGG | 0.562737 | 5_3:-8657545 | Msa0796620:five_prime_UTR |
| ACAGTTCCTTCTTTGCAACA+TGG | 0.577429 | 5_3:+8657422 | None:intergenic |
| CACAAAGAAGAACAAGAAGA+GGG | 0.602024 | 5_3:+8657362 | None:intergenic |
| TTGGGGAAGAGAAGAACCAG+AGG | 0.622299 | 5_3:+8657332 | None:intergenic |
| ACAGAAGAGAACCTTAGTTG+GGG | 0.648692 | 5_3:+8657315 | None:intergenic |
| TAGAAACAAACAAACAAACA+TGG | 0.652612 | 5_3:-8657488 | Msa0796620:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTATATTAGATTGTT+TGG | - | chr5_3:8657388-8657407 | Msa0796620:CDS | 15.0% |
| !! | AAGTTATCATACAATAAAAA+TGG | + | chr5_3:8657558-8657577 | None:intergenic | 15.0% |
| !!! | TTCAATTTTCAGTTTTAGAT+TGG | - | chr5_3:8657232-8657251 | Msa0796620:intron | 20.0% |
| !! | ATTAAAGTAGTTAGCAAATA+TGG | + | chr5_3:8657268-8657287 | None:intergenic | 20.0% |
| !! | ACTATTTCGTGTTTTTCTTT+TGG | + | chr5_3:8656935-8656954 | None:intergenic | 25.0% |
| ! | TAGAAACAAACAAACAAACA+TGG | - | chr5_3:8657033-8657052 | Msa0796620:three_prime_UTR | 25.0% |
| ! | CAGATTACATAATTTGATGA+CGG | - | chr5_3:8657305-8657324 | Msa0796620:intron | 25.0% |
| ! | GAATTTGTGTATCAATAGAT+TGG | - | chr5_3:8657501-8657520 | Msa0796620:CDS | 25.0% |
| ATTTGTACCAACTATATCAC+TGG | - | chr5_3:8657467-8657486 | Msa0796620:CDS | 30.0% | |
| TATATCACTGGAATCTAGAT+AGG | - | chr5_3:8657479-8657498 | Msa0796620:CDS | 30.0% | |
| CTAAATTAGTTGGAGTGGTT+TGG | - | chr5_3:8656981-8657000 | Msa0796620:three_prime_UTR | 35.0% | |
| GATAAGATGAACAGCTTTCT+GGG | + | chr5_3:8657140-8657159 | None:intergenic | 35.0% | |
| CACAAAGAAGAACAAGAAGA+GGG | + | chr5_3:8657162-8657181 | None:intergenic | 35.0% | |
| AACAGAAGAGAACCTTAGTT+GGG | + | chr5_3:8657210-8657229 | None:intergenic | 35.0% | |
| AAACAGAAGAGAACCTTAGT+TGG | + | chr5_3:8657211-8657230 | None:intergenic | 35.0% | |
| ATGCATAGTTTCAGCATAAG+AGG | - | chr5_3:8657328-8657347 | Msa0796620:CDS | 35.0% | |
| CTAGATTCCAGTGATATAGT+TGG | + | chr5_3:8657477-8657496 | None:intergenic | 35.0% | |
| AGCCAGCGAACTAAATTAGT+TGG | - | chr5_3:8656971-8656990 | Msa0796620:three_prime_UTR | 40.0% | |
| ! | CTCCAACTAATTTAGTTCGC+TGG | + | chr5_3:8656976-8656995 | None:intergenic | 40.0% |
| GCGAACTAAATTAGTTGGAG+TGG | - | chr5_3:8656976-8656995 | Msa0796620:three_prime_UTR | 40.0% | |
| CAGTTCCTTCTTTGCAACAT+GGG | + | chr5_3:8657101-8657120 | None:intergenic | 40.0% | |
| ACAGTTCCTTCTTTGCAACA+TGG | + | chr5_3:8657102-8657121 | None:intergenic | 40.0% | |
| GGATAAGATGAACAGCTTTC+TGG | + | chr5_3:8657141-8657160 | None:intergenic | 40.0% | |
| GCACAAAGAAGAACAAGAAG+AGG | + | chr5_3:8657163-8657182 | None:intergenic | 40.0% | |
| TTCTTTGTGCTATCATCCTC+TGG | - | chr5_3:8657173-8657192 | Msa0796620:three_prime_UTR | 40.0% | |
| ACAGAAGAGAACCTTAGTTG+GGG | + | chr5_3:8657209-8657228 | None:intergenic | 40.0% | |
| TTCTTTGCAACATGGGAGAG+AGG | + | chr5_3:8657094-8657113 | None:intergenic | 45.0% | |
| TCTCTCCCATGTTGCAAAGA+AGG | - | chr5_3:8657093-8657112 | Msa0796620:three_prime_UTR | 45.0% | |
| GTTCTTCTCTTCCCCAACTA+AGG | - | chr5_3:8657195-8657214 | Msa0796620:three_prime_UTR | 45.0% | |
| GAGGATCGTATGCTGGTAGT+AGG | + | chr5_3:8657075-8657094 | None:intergenic | 50.0% | |
| TTGGGGAAGAGAAGAACCAG+AGG | + | chr5_3:8657192-8657211 | None:intergenic | 50.0% | |
| TGGGAGAGAGGATCGTATGC+TGG | + | chr5_3:8657082-8657101 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 8656925 | 8657618 | 8656925 | ID=Msa0796620;Name=Msa0796620 |
| chr5_3 | mRNA | 8656925 | 8657618 | 8656925 | ID=Msa0796620-mRNA-1;Parent=Msa0796620;Name=Msa0796620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=97|1|1|1|1|1|2|302|67 |
| chr5_3 | exon | 8656925 | 8657235 | 8656925 | ID=Msa0796620-mRNA-1:exon:3645;Parent=Msa0796620-mRNA-1 |
| chr5_3 | exon | 8657327 | 8657618 | 8657327 | ID=Msa0796620-mRNA-1:exon:3644;Parent=Msa0796620-mRNA-1 |
| chr5_3 | five_prime_UTR | 8657522 | 8657618 | 8657522 | ID=Msa0796620-mRNA-1:five_prime_utr;Parent=Msa0796620-mRNA-1 |
| chr5_3 | CDS | 8657327 | 8657521 | 8657327 | ID=Msa0796620-mRNA-1:cds;Parent=Msa0796620-mRNA-1 |
| chr5_3 | CDS | 8657227 | 8657235 | 8657227 | ID=Msa0796620-mRNA-1:cds;Parent=Msa0796620-mRNA-1 |
| chr5_3 | three_prime_UTR | 8656925 | 8657226 | 8656925 | ID=Msa0796620-mRNA-1:three_prime_utr;Parent=Msa0796620-mRNA-1 |
| Gene Sequence |
| Protein sequence |
