AlfalfaGEDB | Msa1395320

M. sativa cultivar zhongmu-4 / Msa1395320

KEGG: K03012
GO: -
PFAM: PF03874.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1395320	ABN08874.1	97.238	181	5	0	1	181	22	202	1.02e-124	361

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1395320	sp\|Q6DBA5\|NRPD4_ARATH	45.078	193	79	7	1	178	1	181	3.07e-40	139

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1395320	A2Q5H4	97.238	181	5	0	1	181	22	202	4.85e-125	361

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0106650	Msa1395320	0.806748	7.076518e-50	-8.615850e-47
Msa0239760	Msa1395320	0.962957	1.684624e-121	-8.615850e-47
Msa0542360	Msa1395320	-0.803718	3.049325e-49	-8.615850e-47
Msa0598560	Msa1395320	-0.802476	5.509502e-49	-8.615850e-47
Msa0866800	Msa1395320	-0.812228	4.711082e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1395320	MtrunA17_Chr2g0290791	96.685	181	5	1	1	181	1	180	1.93e-126	354
Msa1395320	MtrunA17_Chr8g0338791	40.426	94	54	1	85	178	22	113	8.60e-20	81.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1395320	AT5G09920.1	44.681	94	50	1	85	178	22	113	2.07e-23	91.3

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Find 53 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTCATCAGCAGTTTC+TGG	0.183875	tig0021939:-91175	None:intergenic
AAACTTCATCAGCAGTTTCT+GGG	0.430453	tig0021939:-91174	None:intergenic
AAGAGCGTGTCTAACAGACT+CGG	0.617302	tig0021939:-90876	None:intergenic
AATCTTATATATGACAACAA+AGG	0.660283	tig0021939:+92190	Msa1395320:three_prime_UTR
AATGTCTGACAAAGGAGGAA+AGG	0.587565	tig0021939:+88564	Msa1395320:exon
ACAAGGAATTCAAGATCAAA+TGG	0.440815	tig0021939:+90636	Msa1395320:CDS
ACTCTATGTTCAAATTGATG+TGG	0.554604	tig0021939:-90112	None:intergenic
AGAAAACTTCAAATGCTTGA+TGG	0.417461	tig0021939:+90591	Msa1395320:CDS
AGAAGTTGGACCGTTTGATC+TGG	0.404517	tig0021939:-89881	None:intergenic
AGAGCGTGTCTAACAGACTC+GGG	0.590587	tig0021939:-90875	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGTCAAAACTTCCTCTTT+AGG	-	tig0021939:88547-88566	None:intergenic	30.0%
TTATGATGTTGGGAAATTCT+GGG	+	tig0021939:88751-88770	Msa1395320:intron	30.0%
ATACTTGTACATAGACACTA+TGG	+	tig0021939:88839-88858	Msa1395320:intron	30.0%
CAATACAAAGTCTCAATTGA+GGG	-	tig0021939:89056-89075	None:intergenic	30.0%
ACAATACAAAGTCTCAATTG+AGG	-	tig0021939:89057-89076	None:intergenic	30.0%
CAATTGAGACTTTGTATTGT+CGG	+	tig0021939:89057-89076	Msa1395320:intron	30.0%
CTTAAGTAATTAAAGCGAAG+GGG	-	tig0021939:89239-89258	None:intergenic	30.0%
CACTTAAGTAATTAAAGCGA+AGG	-	tig0021939:89241-89260	None:intergenic	30.0%
ATCATACATGCATAACTACA+CGG	-	tig0021939:89348-89367	None:intergenic	30.0%
GTCTGAGAATTAGTGTTTAT+GGG	+	tig0021939:89439-89458	Msa1395320:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0021939	gene	88282	92274	88282	ID=Msa1395320;Name=Msa1395320
tig0021939	mRNA	88282	92274	88282	ID=Msa1395320-mRNA-1;Parent=Msa1395320;Name=Msa1395320-mRNA-1;_AED=0.19;_eAED=0.19;_QI=99\|0.77\|0.9\|1\|0.88\|0.8\|10\|178\|202
tig0021939	exon	88282	88358	88282	ID=Msa1395320-mRNA-1:exon:14820;Parent=Msa1395320-mRNA-1
tig0021939	exon	88543	88610	88543	ID=Msa1395320-mRNA-1:exon:14821;Parent=Msa1395320-mRNA-1
tig0021939	exon	89659	89727	89659	ID=Msa1395320-mRNA-1:exon:14822;Parent=Msa1395320-mRNA-1
tig0021939	exon	89869	89913	89869	ID=Msa1395320-mRNA-1:exon:14823;Parent=Msa1395320-mRNA-1
tig0021939	exon	90068	90142	90068	ID=Msa1395320-mRNA-1:exon:14824;Parent=Msa1395320-mRNA-1
tig0021939	exon	90584	90695	90584	ID=Msa1395320-mRNA-1:exon:14825;Parent=Msa1395320-mRNA-1
tig0021939	exon	90817	90900	90817	ID=Msa1395320-mRNA-1:exon:14826;Parent=Msa1395320-mRNA-1
tig0021939	exon	91039	91072	91039	ID=Msa1395320-mRNA-1:exon:14827;Parent=Msa1395320-mRNA-1
tig0021939	exon	91151	91219	91151	ID=Msa1395320-mRNA-1:exon:14828;Parent=Msa1395320-mRNA-1
tig0021939	exon	92022	92274	92022	ID=Msa1395320-mRNA-1:exon:14829;Parent=Msa1395320-mRNA-1
tig0021939	five_prime_UTR	88282	88358	88282	ID=Msa1395320-mRNA-1:five_prime_utr;Parent=Msa1395320-mRNA-1
tig0021939	five_prime_UTR	88543	88564	88543	ID=Msa1395320-mRNA-1:five_prime_utr;Parent=Msa1395320-mRNA-1
tig0021939	CDS	88565	88610	88565	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	89659	89727	89659	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	89869	89913	89869	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	90068	90142	90068	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	90584	90695	90584	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	90817	90900	90817	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	91039	91072	91039	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	91151	91219	91151	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	CDS	92022	92096	92022	ID=Msa1395320-mRNA-1:cds;Parent=Msa1395320-mRNA-1
tig0021939	three_prime_UTR	92097	92274	92097	ID=Msa1395320-mRNA-1:three_prime_utr;Parent=Msa1395320-mRNA-1

Gene Sequence

>Msa1395320

ATGTCTGACAAAGGAGGAAAGGGTGGTTCTCTGTTGAGTAAAGGTGGAGGGCTGAAGGGGAAAGATGATAGTGCAACAAAATCAGCAAAGGCAAGGAAAGTTCAGTTTTCCGAAGGTCTATTTGAATCCAGATCAAACGGTCCAACTTCTGGTGGCAAAGGGGACAAGGTTGCAAATGGTGGAAAGAGTTCTGCTGCAAAAGACCCACATCAATTTGAACATAGAGTTGATCAAGAACTACCAGAAAACTTCAAATGCTTGATGGACTGCGAAGCTGCATCTATGTTACAAGGAATTCAAGATCAAATGGTTGCGTTATCAAGAGATCCATCTATCAAAATGCCCGCATCATTTGACAAGGGGCTGCACTATGCCAAAAGCAGCAGCAGCAGCAAGTATTCAAATCCCGAGTCTGTTAGACACGCTCTTGAGCCTCTAATGAATCATGATCTCACCGAGAGCGAGATATGTGTGATTGCTAATGTATGCCCAGAAACTGCTGATGAAGTTTTTGCTCTCCTCCCATCATTAAAGTTTTACCGTTTAGGAGAGCTAGAAGTAATCGCTCTGGCAAATGTAGCACATTCAAAACGCATTGAGACAATATAA

Protein sequence

>Msa1395320

MSDKGGKGGSLLSKGGGLKGKDDSATKSAKARKVQFSEGLFESRSNGPTSGGKGDKVANGGKSSAAKDPHQFEHRVDQELPENFKCLMDCEAASMLQGIQDQMVALSRDPSIKMPASFDKGLHYAKSSSSSKYSNPESVRHALEPLMNHDLTESEICVIANVCPETADEVFALLPSLKFYRLGELEVIALANVAHSKRIETI*