AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1399220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399220 MtrunA17_Chr5g0448701 80.415 337 3 3 1 299 1 312 0.0 533
Msa1399220 MtrunA17_Chr2g0293321 69.972 363 40 7 6 323 1 339 5.36e-169 473
Msa1399220 MtrunA17_Chr2g0293211 43.697 357 144 7 16 319 45 397 3.10e-85 270
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399220 AT1G30680.1 45.875 303 116 4 64 319 107 408 9.53e-80 256
Msa1399220 AT1G30660.2 46.535 303 113 5 64 319 26 326 7.25e-78 241
Msa1399220 AT1G30660.1 46.535 303 113 5 64 319 33 333 8.39e-78 241
Msa1399220 AT1G30660.3 46.535 303 113 5 64 319 23 323 1.04e-77 241

Find 121 sgRNAs with CRISPR-Local

Find 398 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATAGACAAGGTCCTTTA+TGG 0.130566 tig0022346:-23157 Msa1399220:CDS
CGCCCCAAACCCAAAGGTTT+TGG 0.223708 tig0022346:-26358 Msa1399220:CDS
CTTTGTTGATGCTTGTTTGT+AGG 0.263948 tig0022346:+20981 None:intergenic
GAACAATGGAGAAAAGATTT+TGG 0.263962 tig0022346:-23673 Msa1399220:CDS
GCACCAAAACCTTTGGGTTT+GGG 0.271135 tig0022346:+26355 None:intergenic
AAGCTGAGTTCATCTTTCTT+TGG 0.292938 tig0022346:+21696 None:intergenic
AAAGGAGTCTGGAGCTGATT+TGG 0.295968 tig0022346:-20662 Msa1399220:three_prime_UTR
CTTTGTTGATGCTTGTTTGT+AGG 0.313060 tig0022346:+21043 None:intergenic
GGCACCAAAACCTTTGGGTT+TGG 0.313375 tig0022346:+26354 None:intergenic
GCCGTGCCAGGCTTCTGACT+TGG 0.324363 tig0022346:+20599 None:intergenic
GTGTAAAGGTGGACAGTTAA+TGG 0.331736 tig0022346:-25933 Msa1399220:CDS
TGATTGTGCAAGAACAAGTT+TGG 0.339930 tig0022346:-20936 Msa1399220:three_prime_UTR
TGCGATTCTCTACAACATTA+AGG 0.340330 tig0022346:+21265 None:intergenic
CCAGACTTTCGTATGAAACT+TGG 0.341498 tig0022346:-20875 Msa1399220:three_prime_UTR
ACCTAGTGTGCACAAGTATA+TGG 0.342634 tig0022346:+21434 None:intergenic
AAGAGCTAGCACGGCGCCTT+GGG 0.357506 tig0022346:-22289 Msa1399220:CDS
TTGGGGCGAGAAAAGTGATT+TGG 0.360080 tig0022346:+26373 None:intergenic
CCGTGCCAGGCTTCTGACTT+GGG 0.364382 tig0022346:+20600 None:intergenic
ATGAATGGCACCAAAACCTT+TGG 0.365791 tig0022346:+26348 None:intergenic
GAAGAGCTAGCACGGCGCCT+TGG 0.374758 tig0022346:-22290 Msa1399220:CDS
ACCTTAGCACGGGCCGTGCC+AGG 0.387451 tig0022346:+20587 None:intergenic
GTGAAGCCATGTGTCATCTA+AGG 0.394200 tig0022346:+21119 None:intergenic
AACCCTAAGCTCTCCTCATC+AGG 0.396006 tig0022346:+25321 None:intergenic
TACATGTGGTTGGGCTGGTC+AGG 0.399429 tig0022346:-25468 Msa1399220:intron
GTGGAACAGCAAAGCTTACT+TGG 0.403923 tig0022346:-22472 Msa1399220:CDS
ATAATATTTAGTCAAATGTA+AGG 0.424827 tig0022346:-21325 Msa1399220:three_prime_UTR
TGAATGGCACCAAAACCTTT+GGG 0.435245 tig0022346:+26349 None:intergenic
AAACCTGATGAGGAGAGCTT+AGG 0.440096 tig0022346:-25324 Msa1399220:CDS
ACCTTTCCTGGTGCACCATC+AGG 0.440161 tig0022346:+22919 None:intergenic
GTATGAACAATTGTAACTCT+TGG 0.443099 tig0022346:-21370 Msa1399220:three_prime_UTR
CACCAAAACCTTTGGGTTTG+GGG 0.445536 tig0022346:+26356 None:intergenic
TTAGACAACTCTTGTTTGCC+TGG 0.447652 tig0022346:-26075 Msa1399220:CDS
TTAGGTGGTTCGCATTATCC+TGG 0.449392 tig0022346:-22348 Msa1399220:intron
AATAAATTTGATTGTGAACA+AGG 0.453230 tig0022346:-21069 Msa1399220:three_prime_UTR
TTGACCTGGTGGATCATTAT+CGG 0.454060 tig0022346:+22322 None:intergenic
CCAAGTTTCATACGAAAGTC+TGG 0.459369 tig0022346:+20875 None:intergenic
AAACAAAATGGGTTGATTGT+TGG 0.461337 tig0022346:-23707 Msa1399220:CDS
GTTGTAGTGAATTTGCAATC+TGG 0.465654 tig0022346:-25505 Msa1399220:intron
AACCTGATGAGGAGAGCTTA+GGG 0.465798 tig0022346:-25323 Msa1399220:CDS
TAATGTCGTCAAGTCCATAA+AGG 0.465841 tig0022346:+23143 None:intergenic
TTCTGCAGATGACAAGGAAC+TGG 0.466614 tig0022346:-25352 Msa1399220:CDS
TTTATCTAAGAATATGAAGA+TGG 0.467426 tig0022346:-21399 Msa1399220:three_prime_UTR
AAGCTTTCACTTGAAGAGGC+TGG 0.467773 tig0022346:-22964 Msa1399220:CDS
AATCAAACTTATTGTGTTGG+TGG 0.468107 tig0022346:+20954 None:intergenic
AATCAAACTTATTGTGTTGG+TGG 0.468107 tig0022346:+21016 None:intergenic
TGATAAGCTTTCACTTGAAG+AGG 0.469616 tig0022346:-22968 Msa1399220:CDS
CACGGCGCCTTGGGCGAGAA+AGG 0.471934 tig0022346:-22280 Msa1399220:intron
TTAGGATCGCGTTTCAGCTA+TGG 0.472657 tig0022346:+21201 None:intergenic
CGTGCCAGGCTTCTGACTTG+GGG 0.480472 tig0022346:+20601 None:intergenic
GATAATGATAATAGGTTGAG+GGG 0.483543 tig0022346:-22996 Msa1399220:intron
GCCTGGCACGGCCCGTGCTA+AGG 0.485393 tig0022346:-20588 Msa1399220:three_prime_UTR
AGACCTACATGTGGTTGGGC+TGG 0.487821 tig0022346:-25473 Msa1399220:CDS
CAGCAAAGCTTACTTGGATA+AGG 0.491598 tig0022346:-22466 Msa1399220:intron
GTTGGAACCACTTGGTCCTA+AGG 0.491634 tig0022346:-25301 Msa1399220:intron
AGTAAGCTTTGCTGTTCCAC+AGG 0.494786 tig0022346:+22475 None:intergenic
ATATATGATGGAAGAAGTCT+TGG 0.495533 tig0022346:-20693 Msa1399220:three_prime_UTR
TGGAGCAAAAGAAAGGAGTC+TGG 0.500477 tig0022346:-20673 Msa1399220:three_prime_UTR
TGGATACCTTTGGACAAAAG+AGG 0.502264 tig0022346:+26040 None:intergenic
CCTTTGGACAAAAGAGGTTG+TGG 0.510616 tig0022346:+26046 None:intergenic
GGCCTAGGTTCTCAAATACA+TGG 0.513227 tig0022346:-21495 Msa1399220:intron
TGATAATGATAATAGGTTGA+GGG 0.514143 tig0022346:-22997 Msa1399220:intron
ACATGTCAATTTGTCAGAGC+TGG 0.514292 tig0022346:-26415 Msa1399220:CDS
AGCTTAGGGTTGGAACCACT+TGG 0.515652 tig0022346:-25309 Msa1399220:CDS
AAGAATATGAAGATGGATGT+AGG 0.516748 tig0022346:-21392 Msa1399220:three_prime_UTR
TTTCAGGAACGAATATATGA+TGG 0.517370 tig0022346:-20705 Msa1399220:three_prime_UTR
GGCACGGCCCGTGCTAAGGT+TGG 0.520990 tig0022346:-20584 Msa1399220:three_prime_UTR
GGCCGTGCCAACCTTAGCAC+GGG 0.522563 tig0022346:+20577 None:intergenic
GTGGATCATTATCGGTAGCC+AGG 0.524131 tig0022346:+22330 None:intergenic
CACAATCAAACTTATTGTGT+TGG 0.524532 tig0022346:+20951 None:intergenic
CACAATCAAACTTATTGTGT+TGG 0.524532 tig0022346:+21013 None:intergenic
ACGTACGTACGTACCTTTGG+TGG 0.527924 tig0022346:+26316 None:intergenic
AGTGTTCCTGATGGTGCACC+AGG 0.529108 tig0022346:-22925 Msa1399220:CDS
CAGACTTTCGTATGAAACTT+GGG 0.534819 tig0022346:-20874 Msa1399220:three_prime_UTR
TCCTGATGGTGCACCAGGAA+AGG 0.537409 tig0022346:-22920 Msa1399220:intron
CTTCTTCAAAGATGCAAATG+AGG 0.541918 tig0022346:-21676 Msa1399220:intron
GAAGTCTTGGAGCAAAAGAA+AGG 0.544530 tig0022346:-20680 Msa1399220:three_prime_UTR
AAGCACAAAGTGGAACCTCT+TGG 0.547568 tig0022346:-26105 Msa1399220:CDS
TAAAATATTGAAGCACAAAG+TGG 0.551755 tig0022346:-26115 Msa1399220:CDS
CGGCCGTGCCAACCTTAGCA+CGG 0.552838 tig0022346:+20576 None:intergenic
ACAACATTAAGGATCACAAT+GGG 0.557348 tig0022346:+21276 None:intergenic
TACGTACCTTTGGTGGAAAG+TGG 0.559186 tig0022346:+26323 None:intergenic
GGCCCGTGCTAAGGTTGGCA+CGG 0.562700 tig0022346:-20579 Msa1399220:three_prime_UTR
TTGATATGCCAGTGTAAAGG+TGG 0.562981 tig0022346:-25944 Msa1399220:intron
GCCATATACTTGTGCACACT+AGG 0.563415 tig0022346:-21435 Msa1399220:exon
CTTTAGCTGAAGAGCTAGCA+CGG 0.564457 tig0022346:-22298 Msa1399220:CDS
TAGACAACTCTTGTTTGCCT+GGG 0.566831 tig0022346:-26074 Msa1399220:CDS
GTTGTTGCAACTTTAATCGA+CGG 0.568479 tig0022346:-21232 Msa1399220:three_prime_UTR
TCATTACCACTTTCCACCAA+AGG 0.570913 tig0022346:-26329 Msa1399220:intron
AAGAGGTTGTGGTAGTTCCC+AGG 0.571323 tig0022346:+26057 None:intergenic
ATCGCGTTTCAGCTATGGCA+TGG 0.578515 tig0022346:+21206 None:intergenic
TGATGAGGAGAGCTTAGGGT+TGG 0.580112 tig0022346:-25319 Msa1399220:CDS
TGAATGACCTTTCTCGCCCA+AGG 0.584678 tig0022346:+22273 None:intergenic
TCAGGTGTTGGCAAGTACGC+TGG 0.585250 tig0022346:-21719 Msa1399220:intron
TCAGCTAAAGCTTGACCTGG+TGG 0.586602 tig0022346:+22311 None:intergenic
CCCAAGTCAGAAGCCTGGCA+CGG 0.589958 tig0022346:-20600 Msa1399220:three_prime_UTR
AAACTTGGGCAACTGTGGTA+CGG 0.590884 tig0022346:-20860 Msa1399220:three_prime_UTR
GTGCCAGGCTTCTGACTTGG+GGG 0.599410 tig0022346:+20602 None:intergenic
GAGAAAAGTGATTTGGTGAG+AGG 0.600510 tig0022346:+26380 None:intergenic
ATAGGTTTCTGCAGATGACA+AGG 0.602576 tig0022346:-25358 Msa1399220:intron
TCTTCAGCTAAAGCTTGACC+TGG 0.612094 tig0022346:+22308 None:intergenic
TGTCAATTTGTCAGAGCTGG+TGG 0.614230 tig0022346:-26412 Msa1399220:CDS
AAGGTGGACAGTTAATGGAG+AGG 0.614763 tig0022346:-25928 Msa1399220:CDS
GTATGAAACTTGGGCAACTG+TGG 0.615289 tig0022346:-20865 Msa1399220:three_prime_UTR
TGAAGCCATGTGTCATCTAA+GGG 0.625157 tig0022346:+21120 None:intergenic
TGGTGGAAAGTGGTAATGAA+TGG 0.627860 tig0022346:+26333 None:intergenic
TACAACATTAAGGATCACAA+TGG 0.629723 tig0022346:+21275 None:intergenic
AGTCATCCAGAGAGGTGGCA+CGG 0.631316 tig0022346:+20556 None:intergenic
TTGAATACCTTAGGACCAAG+TGG 0.632809 tig0022346:+25294 None:intergenic
TGACCAGCCCAACCACATGT+AGG 0.639589 tig0022346:+25470 None:intergenic
TGGTTGCAAGTACAGAACAA+TGG 0.639955 tig0022346:-23687 Msa1399220:CDS
ATAATGATAATAGGTTGAGG+GGG 0.644644 tig0022346:-22995 Msa1399220:intron
GCCTAGGTTCTCAAATACAT+GGG 0.646477 tig0022346:-21494 Msa1399220:intron
TAGAGAATCGCAGTGAAATG+TGG 0.655696 tig0022346:-21254 Msa1399220:three_prime_UTR
AATTGTGTTAGTGTTCCTGA+TGG 0.656404 tig0022346:-22934 Msa1399220:CDS
ACTGGACAATAAACCTGATG+AGG 0.661447 tig0022346:-25334 Msa1399220:CDS
TTGTCTAAGCTGATACCAAG+AGG 0.662148 tig0022346:+26090 None:intergenic
CTTGGGCAACTGTGGTACGG+AGG 0.664477 tig0022346:-20857 Msa1399220:three_prime_UTR
ATACTGCATATCAATACCTG+TGG 0.667697 tig0022346:-22491 Msa1399220:CDS
GCTACCGATAATGATCCACC+AGG 0.671318 tig0022346:-22326 Msa1399220:CDS
GTAGTGAATTTGCAATCTGG+AGG 0.685376 tig0022346:-25502 Msa1399220:intron
ACAGGGAAAAGTTATAGACA+AGG 0.702128 tig0022346:-23167 Msa1399220:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAAAAATAGTATAA+TGG + tig0022346:22108-22127 None:intergenic 10.0%
!!! TTCTAAAAAAAATAGATTTT+GGG + tig0022346:22884-22903 None:intergenic 10.0%
!!! TTTCTAAAAAAAATAGATTT+TGG + tig0022346:22885-22904 None:intergenic 10.0%
!! TAAAATAATTTGAATACCTT+AGG + tig0022346:21717-21736 None:intergenic 15.0%
!!! TAATTTTCATTAACAATTTG+AGG + tig0022346:22005-22024 None:intergenic 15.0%
!! GAAAATTATCAAAATAATAG+AGG - tig0022346:22017-22036 Msa1399220:intron 15.0%
!! ACATTTATATTCATTTAAGA+TGG + tig0022346:22234-22253 None:intergenic 15.0%
!! CTTAAATGAATATAAATGTA+TGG - tig0022346:22235-22254 Msa1399220:intron 15.0%
!! AAAAATGTAAAACAAGATAT+CGG + tig0022346:22477-22496 None:intergenic 15.0%
!!! ATCTTGTTTTACATTTTTTT+TGG - tig0022346:22479-22498 Msa1399220:CDS 15.0%
!!! TATATTTTTCCAAAATCATT+AGG - tig0022346:22848-22867 Msa1399220:intron 15.0%
!! ATTTATAAAAAACACTAGTA+AGG + tig0022346:23005-23024 None:intergenic 15.0%
!! TTGAATCAATTATGTTAATT+AGG + tig0022346:23524-23543 None:intergenic 15.0%
!!! ATTCAAATGCTAAATTTTTT+TGG - tig0022346:23806-23825 Msa1399220:intron 15.0%
!!! TATAGTTTGATAATGATAAT+AGG - tig0022346:23995-24014 Msa1399220:intron 15.0%
!! AAATATATAATTGAAAGAGA+AGG + tig0022346:24581-24600 None:intergenic 15.0%
!!! TTTCTTTATTTTTTAATGTC+AGG - tig0022346:25262-25281 Msa1399220:intron 15.0%
!! ATATTCTTAGATAAAAATCA+TGG + tig0022346:25595-25614 None:intergenic 15.0%
!! ATAATATTTAGTCAAATGTA+AGG - tig0022346:25674-25693 Msa1399220:intron 15.0%
!!! AATTGCATATTTTATATTTC+AGG - tig0022346:26278-26297 Msa1399220:intron 15.0%
!!! TTTAAATTGTTTTGTGAAGA+TGG - tig0022346:20789-20808 Msa1399220:three_prime_UTR 20.0%
!! TCTATAAAATTACTTATCCA+GGG - tig0022346:21357-21376 Msa1399220:three_prime_UTR 20.0%
!! ACACATATAAATAGAGTAAA+TGG + tig0022346:21458-21477 None:intergenic 20.0%
!!! TTATTTTATTCAGTTTCAGA+AGG - tig0022346:21729-21748 Msa1399220:intron 20.0%
!!! TTATTCCAAAAGTCTTATTT+AGG - tig0022346:21811-21830 Msa1399220:intron 20.0%
!!! TGATTACTATTGATTATTCT+CGG - tig0022346:21850-21869 Msa1399220:intron 20.0%
!! AGATAAAATGCAACTAATAA+AGG + tig0022346:21926-21945 None:intergenic 20.0%
!!! ATTTTATCTGAAGTAAACAT+AGG - tig0022346:21937-21956 Msa1399220:intron 20.0%
!!! TTTTATCTGAAGTAAACATA+GGG - tig0022346:21938-21957 Msa1399220:intron 20.0%
!!! TCACTTGAAATTTTGAAATA+AGG - tig0022346:22042-22061 Msa1399220:intron 20.0%
!! TGAAAGAAATAAAGGAAAAT+GGG + tig0022346:22675-22694 None:intergenic 20.0%
!!! GAACATTTTAAAAGTATATG+AGG + tig0022346:22758-22777 None:intergenic 20.0%
!! TAAATCCTAACGTATAAAAA+TGG + tig0022346:23068-23087 None:intergenic 20.0%
!! GACAACATTATAAAAAAGTA+AGG + tig0022346:23092-23111 None:intergenic 20.0%
!!! CTTTTACCTATAAACAAAAT+GGG - tig0022346:23281-23300 Msa1399220:intron 20.0%
!! TAGCATTTGAATTAAAATCA+GGG + tig0022346:23798-23817 None:intergenic 20.0%
!! TTAGCATTTGAATTAAAATC+AGG + tig0022346:23799-23818 None:intergenic 20.0%
!! AAATGAAGATAACTTCAAAA+AGG - tig0022346:23956-23975 Msa1399220:intron 20.0%
!! AATGAAGATAACTTCAAAAA+GGG - tig0022346:23957-23976 Msa1399220:intron 20.0%
!!! TTGAAACATGTATTTTAGAT+GGG + tig0022346:24429-24448 None:intergenic 20.0%
!!! TTATATATTTCTAGATCAGA+AGG - tig0022346:24591-24610 Msa1399220:intron 20.0%
!!! TATATATTTCTAGATCAGAA+GGG - tig0022346:24592-24611 Msa1399220:intron 20.0%
!!! TATTTTTCATGTTCATAGTA+AGG - tig0022346:24759-24778 Msa1399220:intron 20.0%
!! TTCATTTATTTAGTTGGATA+TGG + tig0022346:25182-25201 None:intergenic 20.0%
!! TACATATAATCATTCAATTG+TGG - tig0022346:25475-25494 Msa1399220:CDS 20.0%
!! TTTATCTAAGAATATGAAGA+TGG - tig0022346:25600-25619 Msa1399220:intron 20.0%
!! AATAAATTTGATTGTGAACA+AGG - tig0022346:25930-25949 Msa1399220:CDS 20.0%
!! TGTAGTAAAAAGATTCAAAA+AGG + tig0022346:26259-26278 None:intergenic 20.0%
! TAAAATATTGAAGCACAAAG+TGG - tig0022346:20884-20903 Msa1399220:three_prime_UTR 25.0%
! GTCTATAAAATTACTTATCC+AGG - tig0022346:21356-21375 Msa1399220:three_prime_UTR 25.0%
!!! AGTTTGATTTGATACATTCA+AGG - tig0022346:21391-21410 Msa1399220:three_prime_UTR 25.0%
!!! ACTCATTTTGGTTCTTAAAT+GGG + tig0022346:21430-21449 None:intergenic 25.0%
!!! TACTCATTTTGGTTCTTAAA+TGG + tig0022346:21431-21450 None:intergenic 25.0%
!! TAAATGGTCATTACTCATTT+TGG + tig0022346:21442-21461 None:intergenic 25.0%
! TCAAACATAGAACTTCATTA+AGG - tig0022346:21588-21607 Msa1399220:intron 25.0%
!! TTACATTTTACTTGATTACG+TGG - tig0022346:21616-21635 Msa1399220:intron 25.0%
! GACAATTTATGACAAAAAGA+AGG + tig0022346:21790-21809 None:intergenic 25.0%
!! AAAGACCTAAATAAGACTTT+TGG + tig0022346:21819-21838 None:intergenic 25.0%
!!! GAAATTTTGAAATAAGGTTC+AGG - tig0022346:22048-22067 Msa1399220:intron 25.0%
! GTGAAAGAAATAAAGGAAAA+TGG + tig0022346:22676-22695 None:intergenic 25.0%
!!! AAATGTTCTAAGACACAATA+TGG - tig0022346:22770-22789 Msa1399220:intron 25.0%
!! TAAGACACAATATGGATTTT+AGG - tig0022346:22778-22797 Msa1399220:intron 25.0%
!! AAGACACAATATGGATTTTA+GGG - tig0022346:22779-22798 Msa1399220:intron 25.0%
!! ATGCAATTGTCACTTAAAAA+TGG + tig0022346:22820-22839 None:intergenic 25.0%
!! AAAGCTAATCCTAATGATTT+TGG + tig0022346:22860-22879 None:intergenic 25.0%
! ATAAAAAACACTAGTAAGGA+GGG + tig0022346:23001-23020 None:intergenic 25.0%
! TATAAAAAACACTAGTAAGG+AGG + tig0022346:23002-23021 None:intergenic 25.0%
!!! TTTGACCATTTTTATACGTT+AGG - tig0022346:23060-23079 Msa1399220:intron 25.0%
!! TTGTAAATATTTTATGTGCG+TGG - tig0022346:23184-23203 Msa1399220:intron 25.0%
! AAGTTAAGAACTAACTAGTT+AGG + tig0022346:23225-23244 None:intergenic 25.0%
!! GCTTTTACCTATAAACAAAA+TGG - tig0022346:23280-23299 Msa1399220:intron 25.0%
!!! AATCAACCCATTTTGTTTAT+AGG + tig0022346:23290-23309 None:intergenic 25.0%
! GTATAATTTCATAACCAGTT+AGG - tig0022346:23449-23468 Msa1399220:intron 25.0%
!!! ATTTTTTTGGAGGATTAATG+TGG - tig0022346:23658-23677 Msa1399220:intron 25.0%
! CATATGAAAATGTATATGCT+TGG + tig0022346:23724-23743 None:intergenic 25.0%
!!! CTAAATTTTTTTGGTGTACA+GGG - tig0022346:23815-23834 Msa1399220:intron 25.0%
! ATTTCCAACAAAAGAAGATA+TGG + tig0022346:23932-23951 None:intergenic 25.0%
!!! TTGATAATGATAATAGGTTG+AGG - tig0022346:24001-24020 Msa1399220:intron 25.0%
!! TGATAATGATAATAGGTTGA+GGG - tig0022346:24002-24021 Msa1399220:intron 25.0%
!! ATAACACAAACCTTTTCTAT+TGG + tig0022346:24125-24144 None:intergenic 25.0%
! CATCACACTAAATATTTACT+AGG + tig0022346:24344-24363 None:intergenic 25.0%
!!! GTTGAAACATGTATTTTAGA+TGG + tig0022346:24430-24449 None:intergenic 25.0%
! GATTGAAGAGATAAAACAAT+TGG + tig0022346:24452-24471 None:intergenic 25.0%
! GAACATGAAAAATAAATGAC+AGG + tig0022346:24753-24772 None:intergenic 25.0%
!!! TCTCAACCATTGATTTAAAT+TGG - tig0022346:25021-25040 Msa1399220:intron 25.0%
!!! AACCATTGATTTAAATTGGA+TGG - tig0022346:25025-25044 Msa1399220:intron 25.0%
! AACCATCCAATTTAAATCAA+TGG + tig0022346:25030-25049 None:intergenic 25.0%
! ATGGAAATATTTGCTCTATA+TGG + tig0022346:25163-25182 None:intergenic 25.0%
! TGTGTCTTCATTTATTTAGT+TGG + tig0022346:25188-25207 None:intergenic 25.0%
!!! TATTTTTTAATGTCAGGTGT+TGG - tig0022346:25268-25287 Msa1399220:intron 25.0%
!! TCTCTATATTTGATTCCTTT+TGG - tig0022346:25368-25387 Msa1399220:intron 25.0%
!! CTAAGAAAATGAACTTTTCA+TGG - tig0022346:25396-25415 Msa1399220:intron 25.0%
! AATCATTCAATTGTGGTTAA+TGG - tig0022346:25482-25501 Msa1399220:CDS 25.0%
! ATCATTCAATTGTGGTTAAT+GGG - tig0022346:25483-25502 Msa1399220:CDS 25.0%
! GTTAACTGAAACAAAAAACA+TGG - tig0022346:26097-26116 Msa1399220:CDS 25.0%
!! ATTGTTTTGTGAAGATGGTT+TGG - tig0022346:20794-20813 Msa1399220:three_prime_UTR 30.0%
ACCAAATACATTGAAAAGAC+TGG + tig0022346:21028-21047 None:intergenic 30.0%
! TTTTTGATATGCCAGTGTAA+AGG - tig0022346:21052-21071 Msa1399220:three_prime_UTR 30.0%
! TCTTTTCACATTATCACTGA+TGG - tig0022346:21100-21119 Msa1399220:three_prime_UTR 30.0%
! CTTTTCACATTATCACTGAT+GGG - tig0022346:21101-21120 Msa1399220:three_prime_UTR 30.0%
!!! TCTTCAACTTGTTTGTTTTG+AGG - tig0022346:21130-21149 Msa1399220:three_prime_UTR 30.0%
!!! ACTTGTTTGTTTTGAGGTTT+TGG - tig0022346:21136-21155 Msa1399220:three_prime_UTR 30.0%
!!! TTTTGAGGTTTTGGTGATAT+TGG - tig0022346:21145-21164 Msa1399220:three_prime_UTR 30.0%
AACAGAAGACACAATATTTG+AGG + tig0022346:21294-21313 None:intergenic 30.0%
TTACTTGATTACGTGGTTAT+AGG - tig0022346:21623-21642 Msa1399220:intron 30.0%
ATAAGTATGGTATGTTCAAG+TGG - tig0022346:22187-22206 Msa1399220:intron 30.0%
TAAGTATGGTATGTTCAAGT+GGG - tig0022346:22188-22207 Msa1399220:intron 30.0%
CTAGTCAGATTATTTGCTTA+TGG - tig0022346:22323-22342 Msa1399220:CDS 30.0%
AACAAGCTAAAATAGGGAAA+AGG + tig0022346:22353-22372 None:intergenic 30.0%
GTAAGCAACAAGCTAAAATA+GGG + tig0022346:22359-22378 None:intergenic 30.0%
AGTAAGCAACAAGCTAAAAT+AGG + tig0022346:22360-22379 None:intergenic 30.0%
!! TTAGCTTGTTGCTTACTTTA+AGG - tig0022346:22363-22382 Msa1399220:intron 30.0%
AATGTAAAACAAGATATCGG+AGG + tig0022346:22474-22493 None:intergenic 30.0%
GGGGAACTTAAAAATTGTAT+TGG + tig0022346:22597-22616 None:intergenic 30.0%
ATAGAAATCTCTCCTCATTT+GGG + tig0022346:22618-22637 None:intergenic 30.0%
AATAGAAATCTCTCCTCATT+TGG + tig0022346:22619-22638 None:intergenic 30.0%
AGAAATAAAGGAAAATGGGA+GGG + tig0022346:22671-22690 None:intergenic 30.0%
AAGAAATAAAGGAAAATGGG+AGG + tig0022346:22672-22691 None:intergenic 30.0%
TCAGCAAGTGAAAGAAATAA+AGG + tig0022346:22683-22702 None:intergenic 30.0%
!! TTTTGACTAATAGTTCTCCT+TGG - tig0022346:22931-22950 Msa1399220:CDS 30.0%
ATTCCAATAGTAAGAGATAG+AGG + tig0022346:22961-22980 None:intergenic 30.0%
TACACTTACAGGATATGATT+AGG + tig0022346:23118-23137 None:intergenic 30.0%
!! AAACAAAATGGGTTGATTGT+TGG - tig0022346:23292-23311 Msa1399220:intron 30.0%
! GAACAATGGAGAAAAGATTT+TGG - tig0022346:23326-23345 Msa1399220:intron 30.0%
!! CAAATTTTAACATGCAAGCA+TGG + tig0022346:23374-23393 None:intergenic 30.0%
TCATTAATGCCACACAATAA+AGG + tig0022346:23484-23503 None:intergenic 30.0%
!!! GAGACGGAAAAGAATTTTTT+TGG - tig0022346:23645-23664 Msa1399220:intron 30.0%
!!! ACGGAAAAGAATTTTTTTGG+AGG - tig0022346:23648-23667 Msa1399220:intron 30.0%
!!! GCTAAATTTTTTTGGTGTAC+AGG - tig0022346:23814-23833 Msa1399220:intron 30.0%
CAACAAAAGAAGATATGGAT+GGG + tig0022346:23927-23946 None:intergenic 30.0%
! GATAATGATAATAGGTTGAG+GGG - tig0022346:24003-24022 Msa1399220:intron 30.0%
! ATAATGATAATAGGTTGAGG+GGG - tig0022346:24004-24023 Msa1399220:intron 30.0%
!! CTTCATAGATTCCACTTTTA+AGG - tig0022346:24318-24337 Msa1399220:intron 30.0%
TATTTACTAGGCCTTAAAAG+TGG + tig0022346:24332-24351 None:intergenic 30.0%
TACTGATGTTTGCATCATTA+AGG + tig0022346:24367-24386 None:intergenic 30.0%
GAAGGGACAACTTATTAATT+TGG - tig0022346:24609-24628 Msa1399220:intron 30.0%
TTGCATTCTCTTGAAATATC+TGG - tig0022346:25137-25156 Msa1399220:intron 30.0%
! ATTTTCTTAGAAGCTCCAAA+AGG + tig0022346:25386-25405 None:intergenic 30.0%
AAGAATATGAAGATGGATGT+AGG - tig0022346:25607-25626 Msa1399220:intron 30.0%
GTATGAACAATTGTAACTCT+TGG - tig0022346:25629-25648 Msa1399220:intron 30.0%
ACAACATTAAGGATCACAAT+GGG + tig0022346:25726-25745 None:intergenic 30.0%
TACAACATTAAGGATCACAA+TGG + tig0022346:25727-25746 None:intergenic 30.0%
! ACGTTTTCTATCAGCTATTT+AGG + tig0022346:25819-25838 None:intergenic 30.0%
AATCAAACTTATTGTGTTGG+TGG + tig0022346:25986-26005 None:intergenic 30.0%
CACAATCAAACTTATTGTGT+TGG + tig0022346:25989-26008 None:intergenic 30.0%
AATCAAACTTATTGTGTTGG+TGG + tig0022346:25986-26005 None:intergenic 30.0%
CACAATCAAACTTATTGTGT+TGG + tig0022346:25989-26008 None:intergenic 30.0%
TTTCAGGAACGAATATATGA+TGG - tig0022346:26294-26313 Msa1399220:intron 30.0%
! ATATATGATGGAAGAAGTCT+TGG - tig0022346:26306-26325 Msa1399220:intron 30.0%
CACTGTTGAATTTCTGATCT+GGG - tig0022346:20714-20733 Msa1399220:three_prime_UTR 35.0%
TCTCTCTACTCAACCAAAAT+GGG - tig0022346:20740-20759 Msa1399220:intron 35.0%
! GAAAACTACATGACCCATTT+TGG + tig0022346:20756-20775 None:intergenic 35.0%
! GCCAGTCTTTTCAATGTATT+TGG - tig0022346:21024-21043 Msa1399220:three_prime_UTR 35.0%
!! AGGTTTTGGTGATATTGGTA+TGG - tig0022346:21150-21169 Msa1399220:three_prime_UTR 35.0%
GACATATTCAAGGATCGAAA+TGG + tig0022346:21192-21211 None:intergenic 35.0%
! GATACACTTGGGGATATTTT+TGG + tig0022346:21252-21271 None:intergenic 35.0%
ACAAGCAAAAGATACACTTG+GGG + tig0022346:21262-21281 None:intergenic 35.0%
GACAAGCAAAAGATACACTT+GGG + tig0022346:21263-21282 None:intergenic 35.0%
TGACAAGCAAAAGATACACT+TGG + tig0022346:21264-21283 None:intergenic 35.0%
GTGTCTTCTGTTAGTTAGTT+TGG - tig0022346:21302-21321 Msa1399220:three_prime_UTR 35.0%
TTATAGACCATGCTGACAAT+TGG + tig0022346:21344-21363 None:intergenic 35.0%
CAAACTTTCAAAAACATCCC+TGG + tig0022346:21377-21396 None:intergenic 35.0%
!!! TTTTGGTTCTTAAATGGGTG+AGG + tig0022346:21425-21444 None:intergenic 35.0%
GTTGTAGTGAATTTGCAATC+TGG - tig0022346:21494-21513 Msa1399220:intron 35.0%
! TTTTAGACCTACATGTGGTT+GGG - tig0022346:21522-21541 Msa1399220:intron 35.0%
GAAGTTCTATGTTTGAGAAC+AGG + tig0022346:21584-21603 None:intergenic 35.0%
!! ACTGCCAATTTTTCAGAATG+AGG - tig0022346:21759-21778 Msa1399220:intron 35.0%
! GCTACCTCATTCTGAAAAAT+TGG + tig0022346:21766-21785 None:intergenic 35.0%
!! TGTGAGTTATGAGTATTGAG+TGG - tig0022346:21896-21915 Msa1399220:intron 35.0%
AATACATTGCAGTAGTAGTG+TGG - tig0022346:22154-22173 Msa1399220:intron 35.0%
!! TGGTCAATGGTTGATAAGTA+TGG - tig0022346:22174-22193 Msa1399220:intron 35.0%
GGCAATGTATTAACTCAACA+TGG + tig0022346:22292-22311 None:intergenic 35.0%
!! GTTAATACATTGCCACTTTC+AGG - tig0022346:22298-22317 Msa1399220:CDS 35.0%
CCTTACAGAATTTCTGCTTA+AGG + tig0022346:22410-22429 None:intergenic 35.0%
CCTTAAGCAGAAATTCTGTA+AGG - tig0022346:22407-22426 Msa1399220:intron 35.0%
AAATTCTGTAAGGCAACTCT+CGG - tig0022346:22417-22436 Msa1399220:intron 35.0%
!!! GGACGAAAAGGTATTTTCAA+TGG - tig0022346:22438-22457 Msa1399220:intron 35.0%
!!! TTTTTAAGTTCCCCCAAATG+AGG - tig0022346:22603-22622 Msa1399220:intron 35.0%
TAGAAATCTCTCCTCATTTG+GGG + tig0022346:22617-22636 None:intergenic 35.0%
GAAATAAAGGAAAATGGGAG+GGG + tig0022346:22670-22689 None:intergenic 35.0%
!! ACTTAAGCTTTGTTTGCAAG+GGG + tig0022346:22730-22749 None:intergenic 35.0%
!! CACTTAAGCTTTGTTTGCAA+GGG + tig0022346:22731-22750 None:intergenic 35.0%
!! ACACTTAAGCTTTGTTTGCA+AGG + tig0022346:22732-22751 None:intergenic 35.0%
CTTCCTCTATCTCTTACTAT+TGG - tig0022346:22955-22974 Msa1399220:CDS 35.0%
AAATGGTCAAATCTCGTCTT+AGG + tig0022346:23051-23070 None:intergenic 35.0%
ATAGCATTGCATACACTTAC+AGG + tig0022346:23129-23148 None:intergenic 35.0%
!! AATGGAGAAAAGATTTTGGC+AGG - tig0022346:23330-23349 Msa1399220:intron 35.0%
AATTTCATAACCAGTTAGGC+AGG - tig0022346:23453-23472 Msa1399220:intron 35.0%
TGATGACTATACGTTAGAGA+CGG - tig0022346:23629-23648 Msa1399220:intron 35.0%
TAATTGGTCCTCCAAATATC+AGG + tig0022346:23690-23709 None:intergenic 35.0%
ATATTTGGAGGACCAATTAG+AGG - tig0022346:23691-23710 Msa1399220:CDS 35.0%
TATTTGGAGGACCAATTAGA+GGG - tig0022346:23692-23711 Msa1399220:CDS 35.0%
ACAGGGAAAAGTTATAGACA+AGG - tig0022346:23832-23851 Msa1399220:intron 35.0%
GTTATAGACAAGGTCCTTTA+TGG - tig0022346:23842-23861 Msa1399220:intron 35.0%
TAATGTCGTCAAGTCCATAA+AGG + tig0022346:23859-23878 None:intergenic 35.0%
TGGATGGGAAAATAGGTTTA+GGG + tig0022346:23912-23931 None:intergenic 35.0%
ATGGATGGGAAAATAGGTTT+AGG + tig0022346:23913-23932 None:intergenic 35.0%
GAAGATATGGATGGGAAAAT+AGG + tig0022346:23919-23938 None:intergenic 35.0%
CCAACAAAAGAAGATATGGA+TGG + tig0022346:23928-23947 None:intergenic 35.0%
! CCATCCATATCTTCTTTTGT+TGG - tig0022346:23925-23944 Msa1399220:intron 35.0%
TGATAAGCTTTCACTTGAAG+AGG - tig0022346:24031-24050 Msa1399220:intron 35.0%
AATTGTGTTAGTGTTCCTGA+TGG - tig0022346:24065-24084 Msa1399220:intron 35.0%
!! TCTTTTGAAGATACCTTTCC+TGG + tig0022346:24095-24114 None:intergenic 35.0%
! ACACAAACCTTTTCTATTGG+TGG + tig0022346:24122-24141 None:intergenic 35.0%
!! GAATCTATGAAGCATTGACA+CGG + tig0022346:24310-24329 None:intergenic 35.0%
ATGCTTGTCTTTATGAAGTC+AGG - tig0022346:24485-24504 Msa1399220:intron 35.0%
ATACTGCATATCAATACCTG+TGG - tig0022346:24508-24527 Msa1399220:intron 35.0%
! TTCCTTTCTCCCATTCTTTT+AGG - tig0022346:24633-24652 Msa1399220:intron 35.0%
TTGAGGTGCAATTAATCGTT+GGG + tig0022346:24881-24900 None:intergenic 35.0%
AAGCTGAGTTCATCTTTCTT+TGG + tig0022346:25306-25325 None:intergenic 35.0%
CTTCTTCAAAGATGCAAATG+AGG - tig0022346:25323-25342 Msa1399220:CDS 35.0%
ATGCAAATGAGGTATAACTC+AGG - tig0022346:25334-25353 Msa1399220:CDS 35.0%
! AAGGTTTTTAATAGCAGTCG+TGG - tig0022346:25693-25712 Msa1399220:intron 35.0%
TGCGATTCTCTACAACATTA+AGG + tig0022346:25737-25756 None:intergenic 35.0%
GTTGTTGCAACTTTAATCGA+CGG - tig0022346:25767-25786 Msa1399220:intron 35.0%
AAAAACCCTTAGATGACACA+TGG - tig0022346:25874-25893 Msa1399220:intron 35.0%
CTTTGTTGATGCTTGTTTGT+AGG + tig0022346:25959-25978 None:intergenic 35.0%
CTTTGTTGATGCTTGTTTGT+AGG + tig0022346:25959-25978 None:intergenic 35.0%
TGATTGTGCAAGAACAAGTT+TGG - tig0022346:26063-26082 Msa1399220:CDS 35.0%
CAGACTTTCGTATGAAACTT+GGG - tig0022346:26125-26144 Msa1399220:CDS 35.0%
ACATGTCAATTTGTCAGAGC+TGG - tig0022346:20584-20603 Msa1399220:three_prime_UTR 40.0%
!! GAGAAAAGTGATTTGGTGAG+AGG + tig0022346:20622-20641 None:intergenic 40.0%
TGAATGGCACCAAAACCTTT+GGG + tig0022346:20653-20672 None:intergenic 40.0%
ATGAATGGCACCAAAACCTT+TGG + tig0022346:20654-20673 None:intergenic 40.0%
! TGGTGGAAAGTGGTAATGAA+TGG + tig0022346:20669-20688 None:intergenic 40.0%
TCATTACCACTTTCCACCAA+AGG - tig0022346:20670-20689 Msa1399220:three_prime_UTR 40.0%
CCAGATCAGAAATTCAACAG+TGG + tig0022346:20716-20735 None:intergenic 40.0%
CCACTGTTGAATTTCTGATC+TGG - tig0022346:20713-20732 Msa1399220:three_prime_UTR 40.0%
CTCTCTCTACTCAACCAAAA+TGG - tig0022346:20739-20758 Msa1399220:intron 40.0%
TTGTCTAAGCTGATACCAAG+AGG + tig0022346:20912-20931 None:intergenic 40.0%
! TTAGACAACTCTTGTTTGCC+TGG - tig0022346:20924-20943 Msa1399220:three_prime_UTR 40.0%
! TAGACAACTCTTGTTTGCCT+GGG - tig0022346:20925-20944 Msa1399220:three_prime_UTR 40.0%
TGGATACCTTTGGACAAAAG+AGG + tig0022346:20962-20981 None:intergenic 40.0%
GGAAGAAAAGTGGATACCTT+TGG + tig0022346:20972-20991 None:intergenic 40.0%
ATAGCTAGGTGGAAGAAAAG+TGG + tig0022346:20982-21001 None:intergenic 40.0%
TTGATATGCCAGTGTAAAGG+TGG - tig0022346:21055-21074 Msa1399220:three_prime_UTR 40.0%
TTAACTGTCCACCTTTACAC+TGG + tig0022346:21066-21085 None:intergenic 40.0%
GTGTAAAGGTGGACAGTTAA+TGG - tig0022346:21066-21085 Msa1399220:three_prime_UTR 40.0%
! GGTGATATTGGTATGGACTA+TGG - tig0022346:21157-21176 Msa1399220:three_prime_UTR 40.0%
GACAACGTCTGACATATTCA+AGG + tig0022346:21202-21221 None:intergenic 40.0%
! GTGTTTTCCAATTGTCAGCA+TGG - tig0022346:21334-21353 Msa1399220:three_prime_UTR 40.0%
CTACAACACACAAATGCAAG+AGG + tig0022346:21481-21500 None:intergenic 40.0%
GTAGTGAATTTGCAATCTGG+AGG - tig0022346:21497-21516 Msa1399220:intron 40.0%
!! AGGTGTTTTAGACCTACATG+TGG - tig0022346:21517-21536 Msa1399220:intron 40.0%
!! GTTTTAGACCTACATGTGGT+TGG - tig0022346:21521-21540 Msa1399220:intron 40.0%
ATAGGTTTCTGCAGATGACA+AGG - tig0022346:21641-21660 Msa1399220:intron 40.0%
ACTGGACAATAAACCTGATG+AGG - tig0022346:21665-21684 Msa1399220:intron 40.0%
TTGAATACCTTAGGACCAAG+TGG + tig0022346:21708-21727 None:intergenic 40.0%
TTGATTATTCTCGGAGCACA+AGG - tig0022346:21859-21878 Msa1399220:intron 40.0%
TTGACCCTTGAACATAAGGA+GGG + tig0022346:22083-22102 None:intergenic 40.0%
TTTGACCCTTGAACATAAGG+AGG + tig0022346:22084-22103 None:intergenic 40.0%
GGATTTGACCCTTGAACATA+AGG + tig0022346:22087-22106 None:intergenic 40.0%
! AAACAGAAGCGGTTCTGATA+AGG + tig0022346:22261-22280 None:intergenic 40.0%
! ATCAGAACCGCTTCTGTTTT+TGG - tig0022346:22262-22281 Msa1399220:intron 40.0%
AGAAATCTCTCCTCATTTGG+GGG + tig0022346:22616-22635 None:intergenic 40.0%
TAAGAGATAGAGGAAGACCA+AGG + tig0022346:22951-22970 None:intergenic 40.0%
!! TATGTGCGTGGTTTAGTTCT+TGG - tig0022346:23196-23215 Msa1399220:intron 40.0%
TGGTTGCAAGTACAGAACAA+TGG - tig0022346:23312-23331 Msa1399220:intron 40.0%
TGTGGTTACAGCCTGATATT+TGG - tig0022346:23676-23695 Msa1399220:CDS 40.0%
CTTGGAGTAAACCCTCTAAT+TGG + tig0022346:23706-23725 None:intergenic 40.0%
AGATCTGCCACCAATAGAAA+AGG - tig0022346:24112-24131 Msa1399220:intron 40.0%
AGTAGCACAGACACTTCTAA+GGG - tig0022346:24172-24191 Msa1399220:intron 40.0%
GATTGAACGTAATCACGTGT+CGG + tig0022346:24220-24239 None:intergenic 40.0%
CAGCAAAGCTTACTTGGATA+AGG - tig0022346:24533-24552 Msa1399220:intron 40.0%
CACCTAAAAGAATGGGAGAA+AGG + tig0022346:24638-24657 None:intergenic 40.0%
! CTTTCTCCCATTCTTTTAGG+TGG - tig0022346:24636-24655 Msa1399220:intron 40.0%
TGCGAACCACCTAAAAGAAT+GGG + tig0022346:24645-24664 None:intergenic 40.0%
ATGCGAACCACCTAAAAGAA+TGG + tig0022346:24646-24665 None:intergenic 40.0%
! TTGACCTGGTGGATCATTAT+CGG + tig0022346:24680-24699 None:intergenic 40.0%
GTGCACATAGTATGTTGCAT+GGG - tig0022346:24819-24838 Msa1399220:intron 40.0%
GTTGAGGTGCAATTAATCGT+TGG + tig0022346:24882-24901 None:intergenic 40.0%
! CTCTGCAATCGATTTACGAT+CGG - tig0022346:24958-24977 Msa1399220:intron 40.0%
! TCTGCAATCGATTTACGATC+GGG - tig0022346:24959-24978 Msa1399220:intron 40.0%
ACAATGCGTTAAGCAGTTGA+GGG + tig0022346:25097-25116 None:intergenic 40.0%
ATGAAGACACATCCTGAATC+CGG - tig0022346:25197-25216 Msa1399220:intron 40.0%
GGACCTAAGAAAGATGAACT+CGG - tig0022346:25230-25249 Msa1399220:intron 40.0%
AAGCCGAGTTCATCTTTCTT+AGG + tig0022346:25236-25255 None:intergenic 40.0%
GCCTAGGTTCTCAAATACAT+GGG - tig0022346:25505-25524 Msa1399220:intron 40.0%
TCCCATGTATTTGAGAACCT+AGG + tig0022346:25509-25528 None:intergenic 40.0%
TAGAGAATCGCAGTGAAATG+TGG - tig0022346:25745-25764 Msa1399220:intron 40.0%
TGAAGCCATGTGTCATCTAA+GGG + tig0022346:25882-25901 None:intergenic 40.0%
CCAAGTTTCATACGAAAGTC+TGG + tig0022346:26127-26146 None:intergenic 40.0%
CCAGACTTTCGTATGAAACT+TGG - tig0022346:26124-26143 Msa1399220:CDS 40.0%
GAAGTCTTGGAGCAAAAGAA+AGG - tig0022346:26319-26338 Msa1399220:intron 40.0%
TGTCAATTTGTCAGAGCTGG+TGG - tig0022346:20587-20606 Msa1399220:three_prime_UTR 45.0%
TTGGGGCGAGAAAAGTGATT+TGG + tig0022346:20629-20648 None:intergenic 45.0%
! CACCAAAACCTTTGGGTTTG+GGG + tig0022346:20646-20665 None:intergenic 45.0%
! GCACCAAAACCTTTGGGTTT+GGG + tig0022346:20647-20666 None:intergenic 45.0%
TACGTACCTTTGGTGGAAAG+TGG + tig0022346:20679-20698 None:intergenic 45.0%
AAGCACAAAGTGGAACCTCT+TGG - tig0022346:20894-20913 Msa1399220:three_prime_UTR 45.0%
CCTTTGGACAAAAGAGGTTG+TGG + tig0022346:20956-20975 None:intergenic 45.0%
! CCACAACCTCTTTTGTCCAA+AGG - tig0022346:20953-20972 Msa1399220:three_prime_UTR 45.0%
CAGCTTAAGTCATAGCTAGG+TGG + tig0022346:20993-21012 None:intergenic 45.0%
CTGCAGCTTAAGTCATAGCT+AGG + tig0022346:20996-21015 None:intergenic 45.0%
CTAGCTATGACTTAAGCTGC+AGG - tig0022346:20994-21013 Msa1399220:three_prime_UTR 45.0%
TGACTTAAGCTGCAGGTAAC+AGG - tig0022346:21001-21020 Msa1399220:three_prime_UTR 45.0%
GACTTAAGCTGCAGGTAACA+GGG - tig0022346:21002-21021 Msa1399220:three_prime_UTR 45.0%
AAGGTGGACAGTTAATGGAG+AGG - tig0022346:21071-21090 Msa1399220:three_prime_UTR 45.0%
TTCTGCAGATGACAAGGAAC+TGG - tig0022346:21647-21666 Msa1399220:intron 45.0%
AAACCTGATGAGGAGAGCTT+AGG - tig0022346:21675-21694 Msa1399220:intron 45.0%
AACCTGATGAGGAGAGCTTA+GGG - tig0022346:21676-21695 Msa1399220:intron 45.0%
TCTACCCCTCCTTATGTTCA+AGG - tig0022346:22075-22094 Msa1399220:intron 45.0%
CTACCCCTCCTTATGTTCAA+GGG - tig0022346:22076-22095 Msa1399220:intron 45.0%
TGACCCTTGAACATAAGGAG+GGG + tig0022346:22082-22101 None:intergenic 45.0%
TGCAGTAGTAGTGTGGTCAA+TGG - tig0022346:22161-22180 Msa1399220:intron 45.0%
GTATGTTCAAGTGGGTACCA+AGG - tig0022346:22196-22215 Msa1399220:intron 45.0%
AGTGGGTACCAAGGAAGTTA+AGG - tig0022346:22205-22224 Msa1399220:intron 45.0%
! GATGGTAGCCTTAACTTCCT+TGG + tig0022346:22216-22235 None:intergenic 45.0%
TGGACAGCCAAAAACAGAAG+CGG + tig0022346:22272-22291 None:intergenic 45.0%
ATCTGACTAGCACCTGAAAG+TGG + tig0022346:22313-22332 None:intergenic 45.0%
ATAAAGGAAAATGGGAGGGG+AGG + tig0022346:22667-22686 None:intergenic 45.0%
!! GAAGGGAGGCTTTGGAAAAA+AGG + tig0022346:22708-22727 None:intergenic 45.0%
!! AGCTTTGTTTGCAAGGGGAA+GGG + tig0022346:22725-22744 None:intergenic 45.0%
!! AAGCTTTGTTTGCAAGGGGA+AGG + tig0022346:22726-22745 None:intergenic 45.0%
CATGCAAGCATGGTTTGAAC+TGG + tig0022346:23364-23383 None:intergenic 45.0%
TTCATAACCAGTTAGGCAGG+TGG - tig0022346:23456-23475 Msa1399220:intron 45.0%
CAGGTGGTTCCTTTATTGTG+TGG - tig0022346:23472-23491 Msa1399220:intron 45.0%
GGTTACAGCCTGATATTTGG+AGG - tig0022346:23679-23698 Msa1399220:CDS 45.0%
AAGCTTTCACTTGAAGAGGC+TGG - tig0022346:24035-24054 Msa1399220:intron 45.0%
CAGTAGCACAGACACTTCTA+AGG - tig0022346:24171-24190 Msa1399220:intron 45.0%
! CTTCTAAGGGAATGTGTGTC+CGG - tig0022346:24185-24204 Msa1399220:intron 45.0%
CACCAGACATGACACTTACA+CGG + tig0022346:24280-24299 None:intergenic 45.0%
!! AGTAAGCTTTGCTGTTCCAC+AGG + tig0022346:24527-24546 None:intergenic 45.0%
GTGGAACAGCAAAGCTTACT+TGG - tig0022346:24527-24546 Msa1399220:intron 45.0%
TTAGGTGGTTCGCATTATCC+TGG - tig0022346:24651-24670 Msa1399220:intron 45.0%
TCTTCAGCTAAAGCTTGACC+TGG + tig0022346:24694-24713 None:intergenic 45.0%
CTTTAGCTGAAGAGCTAGCA+CGG - tig0022346:24701-24720 Msa1399220:intron 45.0%
CGTGCACATAGTATGTTGCA+TGG - tig0022346:24818-24837 Msa1399220:intron 45.0%
ATTGTTACTGCAGGGAATCC+AGG + tig0022346:24911-24930 None:intergenic 45.0%
TGCGTGGTATTGTTACTGCA+GGG + tig0022346:24919-24938 None:intergenic 45.0%
GATTGTTACGAGTAACTGCG+TGG + tig0022346:24935-24954 None:intergenic 45.0%
CACAATGCGTTAAGCAGTTG+AGG + tig0022346:25098-25117 None:intergenic 45.0%
! CAATTGTGGTTAATGGGCCT+AGG - tig0022346:25489-25508 Msa1399220:CDS 45.0%
GGCCTAGGTTCTCAAATACA+TGG - tig0022346:25504-25523 Msa1399220:intron 45.0%
TTAGGATCGCGTTTCAGCTA+TGG + tig0022346:25801-25820 None:intergenic 45.0%
GTGAAGCCATGTGTCATCTA+AGG + tig0022346:25883-25902 None:intergenic 45.0%
GTATGAAACTTGGGCAACTG+TGG - tig0022346:26134-26153 Msa1399220:CDS 45.0%
AAACTTGGGCAACTGTGGTA+CGG - tig0022346:26139-26158 Msa1399220:CDS 45.0%
TGGAGCAAAAGAAAGGAGTC+TGG - tig0022346:26326-26345 Msa1399220:intron 45.0%
! AAAGGAGTCTGGAGCTGATT+TGG - tig0022346:26337-26356 Msa1399220:CDS 45.0%
!!! ATATGATTTTTTTTTTTTTT+TGG - tig0022346:23739-23758 Msa1399220:intron 5.0%
! TTTTCTCGCCCCAAACCCAA+AGG - tig0022346:20635-20654 Msa1399220:three_prime_UTR 50.0%
! GGCACCAAAACCTTTGGGTT+TGG + tig0022346:20648-20667 None:intergenic 50.0%
ACGTACGTACGTACCTTTGG+TGG + tig0022346:20686-20705 None:intergenic 50.0%
CGTACGTACGTACGTACCTT+TGG + tig0022346:20689-20708 None:intergenic 50.0%
AAGAGGTTGTGGTAGTTCCC+AGG + tig0022346:20945-20964 None:intergenic 50.0%
AACCCTAAGCTCTCCTCATC+AGG + tig0022346:21681-21700 None:intergenic 50.0%
TGATGAGGAGAGCTTAGGGT+TGG - tig0022346:21680-21699 Msa1399220:CDS 50.0%
AGCTTAGGGTTGGAACCACT+TGG - tig0022346:21690-21709 Msa1399220:CDS 50.0%
GTTGGAACCACTTGGTCCTA+AGG - tig0022346:21698-21717 Msa1399220:CDS 50.0%
AAGGCAACTCTCGGACGAAA+AGG - tig0022346:22426-22445 Msa1399220:intron 50.0%
AAATGGGAGGGGAGGACTTT+GGG + tig0022346:22659-22678 None:intergenic 50.0%
AAAATGGGAGGGGAGGACTT+TGG + tig0022346:22660-22679 None:intergenic 50.0%
TTTGTTTGCAAGGGGAAGGG+AGG + tig0022346:22722-22741 None:intergenic 50.0%
AAAGGAACCACCTGCCTAAC+TGG + tig0022346:23466-23485 None:intergenic 50.0%
! ACGTAATCACGTGTCGGTGT+CGG + tig0022346:24214-24233 None:intergenic 50.0%
TTACACGGACACGTTGAGAC+TGG + tig0022346:24265-24284 None:intergenic 50.0%
GTCCGTGTAAGTGTCATGTC+TGG - tig0022346:24275-24294 Msa1399220:intron 50.0%
GTGGATCATTATCGGTAGCC+AGG + tig0022346:24672-24691 None:intergenic 50.0%
GCTACCGATAATGATCCACC+AGG - tig0022346:24673-24692 Msa1399220:intron 50.0%
TCAGCTAAAGCTTGACCTGG+TGG + tig0022346:24691-24710 None:intergenic 50.0%
TGAATGACCTTTCTCGCCCA+AGG + tig0022346:24729-24748 None:intergenic 50.0%
TTGCACCTCAACTACAGACC+TGG - tig0022346:24890-24909 Msa1399220:intron 50.0%
GGAATCCAGGTCTGTAGTTG+AGG + tig0022346:24898-24917 None:intergenic 50.0%
CTGCGTGGTATTGTTACTGC+AGG + tig0022346:24920-24939 None:intergenic 50.0%
CTTAGGTCCATTATGAGCGC+CGG + tig0022346:25219-25238 None:intergenic 50.0%
ATCGCGTTTCAGCTATGGCA+TGG + tig0022346:25796-25815 None:intergenic 50.0%
! CGCCCCAAACCCAAAGGTTT+TGG - tig0022346:20641-20660 Msa1399220:three_prime_UTR 55.0%
AGACCTACATGTGGTTGGGC+TGG - tig0022346:21526-21545 Msa1399220:intron 55.0%
TGACCAGCCCAACCACATGT+AGG + tig0022346:21532-21551 None:intergenic 55.0%
!! TACATGTGGTTGGGCTGGTC+AGG - tig0022346:21531-21550 Msa1399220:intron 55.0%
AATGGGAGGGGAGGACTTTG+GGG + tig0022346:22658-22677 None:intergenic 55.0%
! AGTGTTCCTGATGGTGCACC+AGG - tig0022346:24074-24093 Msa1399220:intron 55.0%
! TCCTGATGGTGCACCAGGAA+AGG - tig0022346:24079-24098 Msa1399220:intron 55.0%
ACCTTTCCTGGTGCACCATC+AGG + tig0022346:24083-24102 None:intergenic 55.0%
TTGCGCTCGAACTTGCAGCT+TGG - tig0022346:24846-24865 Msa1399220:intron 55.0%
!! CCATTATGAGCGCCGGATTC+AGG + tig0022346:25212-25231 None:intergenic 55.0%
CCTGAATCCGGCGCTCATAA+TGG - tig0022346:25209-25228 Msa1399220:intron 55.0%
!! TCAGGTGTTGGCAAGTACGC+TGG - tig0022346:25280-25299 Msa1399220:intron 55.0%
ATGGGAGGGGAGGACTTTGG+GGG + tig0022346:22657-22676 None:intergenic 60.0%
TGCAAGGGGAAGGGAGGCTT+TGG + tig0022346:22716-22735 None:intergenic 60.0%
AAGAGCTAGCACGGCGCCTT+GGG - tig0022346:24710-24729 Msa1399220:intron 60.0%
CTTGGGCAACTGTGGTACGG+AGG - tig0022346:26142-26161 Msa1399220:CDS 60.0%
TCTCCCCCAAGTCAGAAGCC+TGG - tig0022346:26394-26413 Msa1399220:CDS 60.0%
!! GTGCCAGGCTTCTGACTTGG+GGG + tig0022346:26400-26419 None:intergenic 60.0%
! CGTGCCAGGCTTCTGACTTG+GGG + tig0022346:26401-26420 None:intergenic 60.0%
CCGTGCCAGGCTTCTGACTT+GGG + tig0022346:26402-26421 None:intergenic 60.0%
CCCAAGTCAGAAGCCTGGCA+CGG - tig0022346:26399-26418 Msa1399220:CDS 60.0%
!! CACGTGTCGGTGTCGGACAC+CGG + tig0022346:24207-24226 None:intergenic 65.0%
GAAGAGCTAGCACGGCGCCT+TGG - tig0022346:24709-24728 Msa1399220:intron 65.0%
GCCGTGCCAGGCTTCTGACT+TGG + tig0022346:26403-26422 None:intergenic 65.0%
GGCCCGTGCTAAGGTTGGCA+CGG - tig0022346:26420-26439 Msa1399220:CDS 65.0%
GGCCGTGCCAACCTTAGCAC+GGG + tig0022346:26425-26444 None:intergenic 65.0%
CGGCCGTGCCAACCTTAGCA+CGG + tig0022346:26426-26445 None:intergenic 65.0%
CACGGCGCCTTGGGCGAGAA+AGG - tig0022346:24719-24738 Msa1399220:intron 70.0%
ACCTTAGCACGGGCCGTGCC+AGG + tig0022346:26415-26434 None:intergenic 70.0%
GGCACGGCCCGTGCTAAGGT+TGG - tig0022346:26415-26434 Msa1399220:CDS 70.0%
GCCTGGCACGGCCCGTGCTA+AGG - tig0022346:26411-26430 Msa1399220:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0022346 gene 20571 26450 20571 ID=Msa1399220;Name=Msa1399220
tig0022346 mRNA 20571 26450 20571 ID=Msa1399220-mRNA-1;Parent=Msa1399220;Name=Msa1399220-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0.75|0.76|1|0.83|0.84|13|832|323
tig0022346 exon 20571 20754 20571 ID=Msa1399220-mRNA-1:exon:17136;Parent=Msa1399220-mRNA-1
tig0022346 exon 20788 21510 20788 ID=Msa1399220-mRNA-1:exon:17135;Parent=Msa1399220-mRNA-1
tig0022346 exon 21677 21737 21677 ID=Msa1399220-mRNA-1:exon:17134;Parent=Msa1399220-mRNA-1
tig0022346 exon 22281 22366 22281 ID=Msa1399220-mRNA-1:exon:17133;Parent=Msa1399220-mRNA-1
tig0022346 exon 22467 22514 22467 ID=Msa1399220-mRNA-1:exon:17132;Parent=Msa1399220-mRNA-1
tig0022346 exon 22921 23004 22921 ID=Msa1399220-mRNA-1:exon:17131;Parent=Msa1399220-mRNA-1
tig0022346 exon 23120 23185 23120 ID=Msa1399220-mRNA-1:exon:17130;Parent=Msa1399220-mRNA-1
tig0022346 exon 23670 23750 23670 ID=Msa1399220-mRNA-1:exon:17129;Parent=Msa1399220-mRNA-1
tig0022346 exon 25302 25376 25302 ID=Msa1399220-mRNA-1:exon:17128;Parent=Msa1399220-mRNA-1
tig0022346 exon 25469 25520 25469 ID=Msa1399220-mRNA-1:exon:17127;Parent=Msa1399220-mRNA-1
tig0022346 exon 25899 25954 25899 ID=Msa1399220-mRNA-1:exon:17126;Parent=Msa1399220-mRNA-1
tig0022346 exon 26047 26213 26047 ID=Msa1399220-mRNA-1:exon:17125;Parent=Msa1399220-mRNA-1
tig0022346 exon 26330 26450 26330 ID=Msa1399220-mRNA-1:exon:17124;Parent=Msa1399220-mRNA-1
tig0022346 CDS 26330 26450 26330 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 26047 26213 26047 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 25899 25954 25899 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 25469 25520 25469 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 25302 25376 25302 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 23670 23750 23670 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 23120 23185 23120 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 22921 23004 22921 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 22467 22514 22467 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 22281 22366 22281 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 21677 21737 21677 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 CDS 21436 21510 21436 ID=Msa1399220-mRNA-1:cds;Parent=Msa1399220-mRNA-1
tig0022346 three_prime_UTR 20788 21435 20788 ID=Msa1399220-mRNA-1:three_prime_utr;Parent=Msa1399220-mRNA-1
tig0022346 three_prime_UTR 20571 20754 20571 ID=Msa1399220-mRNA-1:three_prime_utr;Parent=Msa1399220-mRNA-1
Gene Sequence

>Msa1399220

ATGAAATTGAAAAACATGTCAATTTGTCAGAGCTGGTGGCTATGTCGTCCTCTCACCAAATCACTTTTCTCGCCCCAAACCCAAAGGTTTTGGTGCCATTCATTACCACTTTCCACCAAAGATGGTTTGGAGCATGTTTTTGACAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAGTTAAAATATTGAAGCACAAAGTGGAACCTCTTGGTATCAGCTTAGACAACTCTTGTTTGCCTGGGAACTACCACAACCTCTTTTGTCCAAAGTGTAAAGGTGGACAGTTAATGGAGAGGAGTTTGTCTTTTCACATTATCACTGATGGTGAATTTGCAATCTGGAGGTGTTTTAGACCTACATGTGGTTGGGCTGGTCAGGTTTCTGCAGATGACAAGGAACTGGACAATAAACCTGATGAGGAGAGCTTAGGGTTGGAACCACTTGGTCCTAAGAATGTTATTGCTTTTACCTATAAACAAAATGGGTTGATTGTTGGTTGCAAGTACAGAACAATGGAGAAAAGATTTTGGCAGGGAAAAGTTATAGACAAGGTCCTTTATGGACTTGACGACATTAGTCATGCAACTGAAATCATCATTGTTGAGGGGGAAATTGATAAGCTTTCACTTGAAGAGGCTGGATATCAAAATTGTGTTAGTGTTCCTGATGGTGCACCAGGAAAGGATACTGCATATCAATACCTGTGGAACAGCAAAGCTTACTTGGATAAGGTGGTTCGCATTATCCTGGCTACCGATAATGATCCACCAGGTCAAGCTTTAGCTGAAGAGCTAGCACGGCGCCTTGGGCGAGAAAGGTGTTGGCAAGTACGCTGGCCAAAGAAAGATGAACTCAGCTTCTTCAAAGATGCAAATGAGGTTCTCAAATACATGGGAGCTGATGCTTTGAAGAGAATAGTTGAAAAAGCAGAGCCATATACTTGTGCACACTAG
Protein sequence

>Msa1399220

MKLKNMSICQSWWLCRPLTKSLFSPQTQRFWCHSLPLSTKDGLEHVFDNNNNNNNNNNNNNNNNKVKILKHKVEPLGISLDNSCLPGNYHNLFCPKCKGGQLMERSLSFHIITDGEFAIWRCFRPTCGWAGQVSADDKELDNKPDEESLGLEPLGPKNVIAFTYKQNGLIVGCKYRTMEKRFWQGKVIDKVLYGLDDISHATEIIIVEGEIDKLSLEEAGYQNCVSVPDGAPGKDTAYQYLWNSKAYLDKVVRIILATDNDPPGQALAEELARRLGRERCWQVRWPKKDELSFFKDANEVLKYMGADALKRIVEKAEPYTCAH*