Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410530 | sp|Q9XI31|GLYI4_ARATH | 62.130 | 169 | 59 | 4 | 2 | 167 | 6 | 172 | 1.24e-69 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410530 | G7JCV5 | 96.471 | 170 | 3 | 3 | 1 | 169 | 1 | 168 | 8.35e-113 | 327 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1262190 | Msa1410530 | 0.816272 | 6.024455e-52 | -8.615850e-47 |
| Msa1265300 | Msa1410530 | 0.812142 | 4.918775e-51 | -8.615850e-47 |
| Msa1220850 | Msa1410530 | 0.885382 | 8.530590e-72 | -8.615850e-47 |
| Msa1221270 | Msa1410530 | 0.840761 | 7.158596e-58 | -8.615850e-47 |
| Msa0581350 | Msa1410530 | 0.838702 | 2.458685e-57 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410530 | MtrunA17_Chr4g0063501 | 96.471 | 170 | 3 | 3 | 1 | 169 | 1 | 168 | 1.61e-116 | 327 |
| Msa1410530 | MtrunA17_Chr4g0003581 | 66.026 | 156 | 51 | 2 | 1 | 156 | 5 | 158 | 3.81e-76 | 225 |
| Msa1410530 | MtrunA17_Chr2g0332851 | 62.733 | 161 | 57 | 3 | 1 | 160 | 5 | 163 | 5.87e-67 | 201 |
| Msa1410530 | MtrunA17_Chr2g0332841 | 57.738 | 168 | 66 | 4 | 1 | 167 | 5 | 168 | 2.99e-62 | 189 |
| Msa1410530 | MtrunA17_Chr2g0277151 | 60.432 | 139 | 53 | 1 | 4 | 142 | 2 | 138 | 4.44e-57 | 177 |
| Msa1410530 | MtrunA17_Chr5g0405171 | 57.534 | 146 | 60 | 1 | 2 | 145 | 22 | 167 | 2.23e-56 | 176 |
| Msa1410530 | MtrunA17_Chr8g0372141 | 55.556 | 144 | 62 | 1 | 4 | 145 | 12 | 155 | 1.27e-54 | 171 |
| Msa1410530 | MtrunA17_Chr4g0020201 | 52.143 | 140 | 65 | 2 | 3 | 140 | 32 | 171 | 4.21e-50 | 159 |
| Msa1410530 | MtrunA17_Chr1g0212241 | 54.730 | 148 | 62 | 4 | 20 | 166 | 1 | 144 | 3.28e-49 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410530 | AT1G80160.1 | 63.804 | 163 | 57 | 2 | 2 | 164 | 6 | 166 | 4.97e-73 | 217 |
| Msa1410530 | AT1G80160.3 | 63.804 | 163 | 57 | 2 | 2 | 164 | 47 | 207 | 1.60e-72 | 217 |
| Msa1410530 | AT1G15380.2 | 62.130 | 169 | 59 | 4 | 2 | 167 | 6 | 172 | 1.26e-70 | 211 |
| Msa1410530 | AT1G15380.1 | 62.130 | 169 | 59 | 4 | 2 | 167 | 6 | 172 | 1.26e-70 | 211 |
| Msa1410530 | AT2G28420.1 | 51.852 | 135 | 63 | 2 | 7 | 140 | 18 | 151 | 4.63e-51 | 162 |
| Msa1410530 | AT1G80160.2 | 59.350 | 123 | 48 | 2 | 42 | 164 | 20 | 140 | 2.31e-45 | 146 |
Find 28 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATTCTTGGTCATACTTTC+TGG | 0.158615 | tig0023670:-43708 | None:intergenic |
| TTGATTAGGTTGTTTAATTA+TGG | 0.210559 | tig0023670:+43651 | Msa1410530:intron |
| AAGAACAGAGTAGAGGAAAA+TGG | 0.294782 | tig0023670:+43920 | Msa1410530:CDS |
| ATTAATGTGCACATTATTCT+TGG | 0.304684 | tig0023670:-43721 | None:intergenic |
| CAAATTGTGTTCTCTGATAA+AGG | 0.332337 | tig0023670:-44025 | None:intergenic |
| GATATACTTGGAAAGAAATA+TGG | 0.341030 | tig0023670:-43755 | None:intergenic |
| TTCTCTGATAAAGGTACAAT+TGG | 0.358152 | tig0023670:-44016 | None:intergenic |
| AAAGAAATATGGTTGTCCTT+TGG | 0.368432 | tig0023670:-43744 | None:intergenic |
| AAGGAGGTAGGTCTCTTAAT+AGG | 0.373287 | tig0023670:-43506 | None:intergenic |
| CTTTATCAGAGAACACAATT+TGG | 0.412500 | tig0023670:+44026 | Msa1410530:CDS |
| TTTCTGGATCATCATCTGAT+TGG | 0.413129 | tig0023670:-43692 | None:intergenic |
| TCGTTGCTAGTGTGAAAGTA+TGG | 0.421471 | tig0023670:+43853 | Msa1410530:intron |
| ATCATTGAATCACATCTCAT+TGG | 0.477448 | tig0023670:+43427 | Msa1410530:CDS |
| ATTCAATGATTTGAGTTGTA+GGG | 0.480431 | tig0023670:-43414 | None:intergenic |
| ATTTCAATCATAGTGCCATC+TGG | 0.481430 | tig0023670:-43974 | None:intergenic |
| TCATAGTGCCATCTGGATCA+TGG | 0.483510 | tig0023670:-43967 | None:intergenic |
| CCTACCTCCTTAGTCTTCAA+TGG | 0.495557 | tig0023670:+43518 | Msa1410530:CDS |
| AGGTTGTTTAATTATGGCAT+TGG | 0.497038 | tig0023670:+43657 | Msa1410530:intron |
| GATTCAATGATTTGAGTTGT+AGG | 0.509824 | tig0023670:-43415 | None:intergenic |
| GTGTGAAAGTATGGCAGTAG+TGG | 0.540760 | tig0023670:+43862 | Msa1410530:intron |
| CCATGCACCATTGAAGACTA+AGG | 0.547692 | tig0023670:-43525 | None:intergenic |
| TTCAATGATTTGAGTTGTAG+GGG | 0.553026 | tig0023670:-43413 | None:intergenic |
| GTATGTGAAGAACAGAGTAG+AGG | 0.586484 | tig0023670:+43913 | Msa1410530:CDS |
| TTGTTCTTCCATGATCCAGA+TGG | 0.596741 | tig0023670:+43959 | Msa1410530:CDS |
| CCTTAGTCTTCAATGGTGCA+TGG | 0.608606 | tig0023670:+43525 | Msa1410530:CDS |
| TAATGTGCACATTAATCCAA+AGG | 0.618094 | tig0023670:+43728 | Msa1410530:CDS |
| CCATTGAAGACTAAGGAGGT+AGG | 0.633678 | tig0023670:-43518 | None:intergenic |
| TGCACCATTGAAGACTAAGG+AGG | 0.725875 | tig0023670:-43522 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAACTTATGTTTACATTTT+TGG | + | tig0023670:43627-43646 | Msa1410530:intron | 15.0% |
| !! | AAATAATTTACACAAATAGA+GGG | - | tig0023670:43786-43805 | None:intergenic | 15.0% |
| !! | AAATCACATAATAAGTATAT+AGG | - | tig0023670:43829-43848 | None:intergenic | 15.0% |
| !! | AACATAAGTTATTCTAACAA+TGG | - | tig0023670:43619-43638 | None:intergenic | 20.0% |
| !!! | TTTACATTTTTGGTTTGATT+AGG | + | tig0023670:43637-43656 | Msa1410530:intron | 20.0% |
| !!! | TTGATTAGGTTGTTTAATTA+TGG | + | tig0023670:43651-43670 | Msa1410530:intron | 20.0% |
| !! | GAAATAATTTACACAAATAG+AGG | - | tig0023670:43787-43806 | None:intergenic | 20.0% |
| !!! | ATTCAATGATTTGAGTTGTA+GGG | - | tig0023670:43417-43436 | None:intergenic | 25.0% |
| ! | ATTAATGTGCACATTATTCT+TGG | - | tig0023670:43724-43743 | None:intergenic | 25.0% |
| ! | GATATACTTGGAAAGAAATA+TGG | - | tig0023670:43758-43777 | None:intergenic | 25.0% |
| !! | TTCAATGATTTGAGTTGTAG+GGG | - | tig0023670:43416-43435 | None:intergenic | 30.0% |
| !! | GATTCAATGATTTGAGTTGT+AGG | - | tig0023670:43418-43437 | None:intergenic | 30.0% |
| ATCATTGAATCACATCTCAT+TGG | + | tig0023670:43427-43446 | Msa1410530:CDS | 30.0% | |
| ! | GATTTTTACGTCAATGTTCT+TGG | + | tig0023670:43476-43495 | Msa1410530:CDS | 30.0% |
| AGGTTGTTTAATTATGGCAT+TGG | + | tig0023670:43657-43676 | Msa1410530:intron | 30.0% | |
| TTATTCTTGGTCATACTTTC+TGG | - | tig0023670:43711-43730 | None:intergenic | 30.0% | |
| TAATGTGCACATTAATCCAA+AGG | + | tig0023670:43728-43747 | Msa1410530:CDS | 30.0% | |
| AAAGAAATATGGTTGTCCTT+TGG | - | tig0023670:43747-43766 | None:intergenic | 30.0% | |
| ! | TTCTCTGATAAAGGTACAAT+TGG | - | tig0023670:44019-44038 | None:intergenic | 30.0% |
| CAAATTGTGTTCTCTGATAA+AGG | - | tig0023670:44028-44047 | None:intergenic | 30.0% | |
| CTTTATCAGAGAACACAATT+TGG | + | tig0023670:44026-44045 | Msa1410530:CDS | 30.0% | |
| TTTCTGGATCATCATCTGAT+TGG | - | tig0023670:43695-43714 | None:intergenic | 35.0% | |
| AAGAACAGAGTAGAGGAAAA+TGG | + | tig0023670:43920-43939 | Msa1410530:CDS | 35.0% | |
| ATTTCAATCATAGTGCCATC+TGG | - | tig0023670:43977-43996 | None:intergenic | 35.0% | |
| AAGGAGGTAGGTCTCTTAAT+AGG | - | tig0023670:43509-43528 | None:intergenic | 40.0% | |
| TAGAGGGAGAGAGATATACT+TGG | - | tig0023670:43770-43789 | None:intergenic | 40.0% | |
| TCGTTGCTAGTGTGAAAGTA+TGG | + | tig0023670:43853-43872 | Msa1410530:intron | 40.0% | |
| GTATGTGAAGAACAGAGTAG+AGG | + | tig0023670:43913-43932 | Msa1410530:CDS | 40.0% | |
| TTGTTCTTCCATGATCCAGA+TGG | + | tig0023670:43959-43978 | Msa1410530:CDS | 40.0% | |
| CCATTGAAGACTAAGGAGGT+AGG | - | tig0023670:43521-43540 | None:intergenic | 45.0% | |
| ! | CCTACCTCCTTAGTCTTCAA+TGG | + | tig0023670:43518-43537 | Msa1410530:CDS | 45.0% |
| TGCACCATTGAAGACTAAGG+AGG | - | tig0023670:43525-43544 | None:intergenic | 45.0% | |
| CCATGCACCATTGAAGACTA+AGG | - | tig0023670:43528-43547 | None:intergenic | 45.0% | |
| ! | CCTTAGTCTTCAATGGTGCA+TGG | + | tig0023670:43525-43544 | Msa1410530:CDS | 45.0% |
| ! | GTGTGAAAGTATGGCAGTAG+TGG | + | tig0023670:43862-43881 | Msa1410530:intron | 45.0% |
| TCATAGTGCCATCTGGATCA+TGG | - | tig0023670:43970-43989 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023670 | gene | 43404 | 44117 | 43404 | ID=Msa1410530;Name=Msa1410530 |
| tig0023670 | mRNA | 43404 | 44117 | 43404 | ID=Msa1410530-mRNA-1;Parent=Msa1410530;Name=Msa1410530-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|3|0|169 |
| tig0023670 | exon | 43404 | 43546 | 43404 | ID=Msa1410530-mRNA-1:exon:26614;Parent=Msa1410530-mRNA-1 |
| tig0023670 | exon | 43659 | 43770 | 43659 | ID=Msa1410530-mRNA-1:exon:26615;Parent=Msa1410530-mRNA-1 |
| tig0023670 | exon | 43863 | 44117 | 43863 | ID=Msa1410530-mRNA-1:exon:26616;Parent=Msa1410530-mRNA-1 |
| tig0023670 | CDS | 43404 | 43546 | 43404 | ID=Msa1410530-mRNA-1:cds;Parent=Msa1410530-mRNA-1 |
| tig0023670 | CDS | 43659 | 43770 | 43659 | ID=Msa1410530-mRNA-1:cds;Parent=Msa1410530-mRNA-1 |
| tig0023670 | CDS | 43863 | 44117 | 43863 | ID=Msa1410530-mRNA-1:cds;Parent=Msa1410530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
