AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1262190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262190 MtrunA17_Chr4g0063501 95.238 168 7 1 1 167 1 168 5.40e-110 310
Msa1262190 MtrunA17_Chr4g0003581 67.532 154 50 0 1 154 5 158 3.51e-79 232
Msa1262190 MtrunA17_Chr2g0332851 60.479 167 63 2 1 166 5 169 1.68e-69 207
Msa1262190 MtrunA17_Chr2g0332841 58.434 166 66 2 1 165 5 168 2.35e-66 200
Msa1262190 MtrunA17_Chr2g0277151 60.584 137 54 0 4 140 2 138 1.98e-57 177
Msa1262190 MtrunA17_Chr5g0405171 54.795 146 62 1 2 143 22 167 9.21e-56 174
Msa1262190 MtrunA17_Chr8g0372141 54.422 147 59 2 3 143 11 155 3.39e-54 170
Msa1262190 MtrunA17_Chr1g0212241 54.795 146 63 2 20 164 1 144 6.74e-52 162
Msa1262190 MtrunA17_Chr4g0020201 52.206 136 61 2 7 138 36 171 1.61e-49 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262190 AT1G80160.1 65.839 161 55 0 2 162 6 166 9.51e-78 229
Msa1262190 AT1G80160.3 65.839 161 55 0 2 162 47 207 3.16e-77 229
Msa1262190 AT1G15380.2 62.275 167 60 2 2 165 6 172 2.55e-73 218
Msa1262190 AT1G15380.1 62.275 167 60 2 2 165 6 172 2.55e-73 218
Msa1262190 AT2G28420.1 50.714 140 67 2 1 138 12 151 8.37e-52 164
Msa1262190 AT1G80160.2 65.789 114 39 0 49 162 27 140 2.66e-50 158

Find 29 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATTCTTGGTCATACTTTC+TGG 0.185353 8_2:+64809052 None:intergenic
TTGATTAGGTTGTTTAATTA+TGG 0.210559 8_2:-64809106 Msa1262190:intron
TTGGCTTCTTTCCTGTTAAA+AGG 0.248281 8_2:-64809309 Msa1262190:CDS
TGGATTAATGTGCACATTCT+TGG 0.286505 8_2:+64809039 None:intergenic
AAGAACAGAGTAGAGGAAAA+TGG 0.294782 8_2:-64808840 Msa1262190:CDS
CATTCTTGGTCATACTTTCT+GGG 0.322846 8_2:+64809053 None:intergenic
ATCATTGAATCACATTTCAT+TGG 0.362713 8_2:-64809377 Msa1262190:CDS
TACTTTCTGGGTCATCTGAT+TGG 0.372059 8_2:+64809065 None:intergenic
TTCTCTGATAGAGGTACAAC+TGG 0.404856 8_2:+64808744 None:intergenic
AAAGAAATGTGGTTGTCCTT+TGG 0.424055 8_2:+64809019 None:intergenic
ACAATTGCAGATTTCAATCA+TGG 0.433463 8_2:+64808776 None:intergenic
ATTTCAATCATGGTGCCATC+TGG 0.434106 8_2:+64808786 None:intergenic
CTCTATCAGAGAACACAATT+TGG 0.449396 8_2:-64808734 Msa1262190:CDS
TACGTACCATGCACCATTGA+AGG 0.464268 8_2:+64809273 None:intergenic
CAAATTGTGTTCTCTGATAG+AGG 0.467908 8_2:+64808735 None:intergenic
CTGGGTCATCTGATTGGAGA+AGG 0.480699 8_2:+64809071 None:intergenic
TTGTTGCTAGTGTGAAAGTA+TGG 0.480825 8_2:-64808907 Msa1262190:intron
CCTACCTCCTTAGCCTTCAA+TGG 0.490822 8_2:-64809286 Msa1262190:CDS
TCATGGTGCCATCTGGATCA+TGG 0.496370 8_2:+64808793 None:intergenic
AGGTTGTTTAATTATGGCAT+TGG 0.497038 8_2:-64809100 Msa1262190:CDS
CCATGCACCATTGAAGGCTA+AGG 0.575830 8_2:+64809279 None:intergenic
GTATGTGAAGAACAGAGTAG+AGG 0.586484 8_2:-64808847 Msa1262190:CDS
CCATTGAAGGCTAAGGAGGT+AGG 0.594731 8_2:+64809286 None:intergenic
TTGTTCTTCCATGATCCAGA+TGG 0.596741 8_2:-64808801 Msa1262190:CDS
GTGTGAAAGTATGGTAGCAG+TGG 0.597656 8_2:-64808898 Msa1262190:CDS
GATATACTTGGAAAGAAATG+TGG 0.600017 8_2:+64809008 None:intergenic
CCTTAGCCTTCAATGGTGCA+TGG 0.610902 8_2:-64809279 Msa1262190:intron
GAATGTGCACATTAATCCAA+AGG 0.612046 8_2:-64809035 Msa1262190:CDS
TGCACCATTGAAGGCTAAGG+AGG 0.624890 8_2:+64809282 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATAAGTTATTTTAACAA+TGG + chr8_2:64808876-64808895 None:intergenic 10.0%
!!! TATATAAGTTATTTTAACAA+TGG + chr8_2:64808876-64808895 None:intergenic 10.0%
!! AATTTACACAAATAAATAGA+GGG + chr8_2:64809090-64809109 None:intergenic 15.0%
!! TAATTTACACAAATAAATAG+AGG + chr8_2:64809091-64809110 None:intergenic 15.0%
!! AATTTACACAAATAAATAGA+GGG + chr8_2:64809090-64809109 None:intergenic 15.0%
!! TAATTTACACAAATAAATAG+AGG + chr8_2:64809091-64809110 None:intergenic 15.0%
!!! ATTCAATGATTTTAGTTGAA+GGG + chr8_2:64808678-64808697 None:intergenic 20.0%
!! GATTCATCAACATAATATTT+AGG + chr8_2:64808843-64808862 None:intergenic 20.0%
!! AGTATCAAAATTCAATGAAA+AGG + chr8_2:64808924-64808943 None:intergenic 20.0%
!!! TTTTGATACTAATTGCATTT+TGG - chr8_2:64808934-64808953 Msa1262190:intron 20.0%
!!! TTTGATACTAATTGCATTTT+GGG - chr8_2:64808935-64808954 Msa1262190:intron 20.0%
!!! TTGATTAGGTTGTTTAATTA+TGG - chr8_2:64808959-64808978 Msa1262190:intron 20.0%
!!! ATTCAATGATTTTAGTTGAA+GGG + chr8_2:64808678-64808697 None:intergenic 20.0%
!! GATTCATCAACATAATATTT+AGG + chr8_2:64808843-64808862 None:intergenic 20.0%
!! AGTATCAAAATTCAATGAAA+AGG + chr8_2:64808924-64808943 None:intergenic 20.0%
!!! TTTTGATACTAATTGCATTT+TGG - chr8_2:64808934-64808953 Msa1262190:intron 20.0%
!!! TTTGATACTAATTGCATTTT+GGG - chr8_2:64808935-64808954 Msa1262190:intron 20.0%
!!! TTGATTAGGTTGTTTAATTA+TGG - chr8_2:64808959-64808978 Msa1262190:intron 20.0%
!!! TTCAATGATTTTAGTTGAAG+GGG + chr8_2:64808677-64808696 None:intergenic 25.0%
!!! GATTCAATGATTTTAGTTGA+AGG + chr8_2:64808679-64808698 None:intergenic 25.0%
! ATCATTGAATCACATTTCAT+TGG - chr8_2:64808688-64808707 Msa1262190:CDS 25.0%
!!! TTCAATGATTTTAGTTGAAG+GGG + chr8_2:64808677-64808696 None:intergenic 25.0%
!!! GATTCAATGATTTTAGTTGA+AGG + chr8_2:64808679-64808698 None:intergenic 25.0%
! ATCATTGAATCACATTTCAT+TGG - chr8_2:64808688-64808707 Msa1262190:CDS 25.0%
! GATTTTTACGTCAATGTTCT+TGG - chr8_2:64808737-64808756 Msa1262190:CDS 30.0%
!!! ATTGCATTTTGGGTTTGATT+AGG - chr8_2:64808945-64808964 Msa1262190:intron 30.0%
AGGTTGTTTAATTATGGCAT+TGG - chr8_2:64808965-64808984 Msa1262190:intron 30.0%
GATATACTTGGAAAGAAATG+TGG + chr8_2:64809060-64809079 None:intergenic 30.0%
ACAATTGCAGATTTCAATCA+TGG + chr8_2:64809292-64809311 None:intergenic 30.0%
! GATTTTTACGTCAATGTTCT+TGG - chr8_2:64808737-64808756 Msa1262190:CDS 30.0%
!!! ATTGCATTTTGGGTTTGATT+AGG - chr8_2:64808945-64808964 Msa1262190:intron 30.0%
AGGTTGTTTAATTATGGCAT+TGG - chr8_2:64808965-64808984 Msa1262190:intron 30.0%
GATATACTTGGAAAGAAATG+TGG + chr8_2:64809060-64809079 None:intergenic 30.0%
ACAATTGCAGATTTCAATCA+TGG + chr8_2:64809292-64809311 None:intergenic 30.0%
TTGGCTTCTTTCCTGTTAAA+AGG - chr8_2:64808756-64808775 Msa1262190:CDS 35.0%
CATTCTTGGTCATACTTTCT+GGG + chr8_2:64809015-64809034 None:intergenic 35.0%
ACATTCTTGGTCATACTTTC+TGG + chr8_2:64809016-64809035 None:intergenic 35.0%
TGGATTAATGTGCACATTCT+TGG + chr8_2:64809029-64809048 None:intergenic 35.0%
GAATGTGCACATTAATCCAA+AGG - chr8_2:64809030-64809049 Msa1262190:CDS 35.0%
AAAGAAATGTGGTTGTCCTT+TGG + chr8_2:64809049-64809068 None:intergenic 35.0%
TTGTTGCTAGTGTGAAAGTA+TGG - chr8_2:64809158-64809177 Msa1262190:intron 35.0%
AAGAACAGAGTAGAGGAAAA+TGG - chr8_2:64809225-64809244 Msa1262190:intron 35.0%
CAAATTGTGTTCTCTGATAG+AGG + chr8_2:64809333-64809352 None:intergenic 35.0%
CTCTATCAGAGAACACAATT+TGG - chr8_2:64809331-64809350 Msa1262190:CDS 35.0%
TTGGCTTCTTTCCTGTTAAA+AGG - chr8_2:64808756-64808775 Msa1262190:CDS 35.0%
CATTCTTGGTCATACTTTCT+GGG + chr8_2:64809015-64809034 None:intergenic 35.0%
ACATTCTTGGTCATACTTTC+TGG + chr8_2:64809016-64809035 None:intergenic 35.0%
TGGATTAATGTGCACATTCT+TGG + chr8_2:64809029-64809048 None:intergenic 35.0%
GAATGTGCACATTAATCCAA+AGG - chr8_2:64809030-64809049 Msa1262190:CDS 35.0%
AAAGAAATGTGGTTGTCCTT+TGG + chr8_2:64809049-64809068 None:intergenic 35.0%
TTGTTGCTAGTGTGAAAGTA+TGG - chr8_2:64809158-64809177 Msa1262190:intron 35.0%
AAGAACAGAGTAGAGGAAAA+TGG - chr8_2:64809225-64809244 Msa1262190:intron 35.0%
CAAATTGTGTTCTCTGATAG+AGG + chr8_2:64809333-64809352 None:intergenic 35.0%
CTCTATCAGAGAACACAATT+TGG - chr8_2:64809331-64809350 Msa1262190:CDS 35.0%
TACTTTCTGGGTCATCTGAT+TGG + chr8_2:64809003-64809022 None:intergenic 40.0%
GAGGGAGAGAATGATATACT+TGG + chr8_2:64809072-64809091 None:intergenic 40.0%
GTATGTGAAGAACAGAGTAG+AGG - chr8_2:64809218-64809237 Msa1262190:intron 40.0%
TTGTTCTTCCATGATCCAGA+TGG - chr8_2:64809264-64809283 Msa1262190:intron 40.0%
!! ATTTCAATCATGGTGCCATC+TGG + chr8_2:64809282-64809301 None:intergenic 40.0%
! TTCTCTGATAGAGGTACAAC+TGG + chr8_2:64809324-64809343 None:intergenic 40.0%
TACTTTCTGGGTCATCTGAT+TGG + chr8_2:64809003-64809022 None:intergenic 40.0%
GAGGGAGAGAATGATATACT+TGG + chr8_2:64809072-64809091 None:intergenic 40.0%
GTATGTGAAGAACAGAGTAG+AGG - chr8_2:64809218-64809237 Msa1262190:intron 40.0%
TTGTTCTTCCATGATCCAGA+TGG - chr8_2:64809264-64809283 Msa1262190:intron 40.0%
!! ATTTCAATCATGGTGCCATC+TGG + chr8_2:64809282-64809301 None:intergenic 40.0%
! TTCTCTGATAGAGGTACAAC+TGG + chr8_2:64809324-64809343 None:intergenic 40.0%
! AAGGAGGTAGGCCTTTTAAC+AGG + chr8_2:64808770-64808789 None:intergenic 45.0%
TACGTACCATGCACCATTGA+AGG + chr8_2:64808795-64808814 None:intergenic 45.0%
! GTGTGAAAGTATGGTAGCAG+TGG - chr8_2:64809167-64809186 Msa1262190:intron 45.0%
! AAGGAGGTAGGCCTTTTAAC+AGG + chr8_2:64808770-64808789 None:intergenic 45.0%
TACGTACCATGCACCATTGA+AGG + chr8_2:64808795-64808814 None:intergenic 45.0%
! GTGTGAAAGTATGGTAGCAG+TGG - chr8_2:64809167-64809186 Msa1262190:intron 45.0%
CCATTGAAGGCTAAGGAGGT+AGG + chr8_2:64808782-64808801 None:intergenic 50.0%
! CCTACCTCCTTAGCCTTCAA+TGG - chr8_2:64808779-64808798 Msa1262190:CDS 50.0%
TGCACCATTGAAGGCTAAGG+AGG + chr8_2:64808786-64808805 None:intergenic 50.0%
CCATGCACCATTGAAGGCTA+AGG + chr8_2:64808789-64808808 None:intergenic 50.0%
! CCTTAGCCTTCAATGGTGCA+TGG - chr8_2:64808786-64808805 Msa1262190:CDS 50.0%
CTGGGTCATCTGATTGGAGA+AGG + chr8_2:64808997-64809016 None:intergenic 50.0%
!! TCATGGTGCCATCTGGATCA+TGG + chr8_2:64809275-64809294 None:intergenic 50.0%
CCATTGAAGGCTAAGGAGGT+AGG + chr8_2:64808782-64808801 None:intergenic 50.0%
! CCTACCTCCTTAGCCTTCAA+TGG - chr8_2:64808779-64808798 Msa1262190:CDS 50.0%
TGCACCATTGAAGGCTAAGG+AGG + chr8_2:64808786-64808805 None:intergenic 50.0%
CCATGCACCATTGAAGGCTA+AGG + chr8_2:64808789-64808808 None:intergenic 50.0%
! CCTTAGCCTTCAATGGTGCA+TGG - chr8_2:64808786-64808805 Msa1262190:CDS 50.0%
CTGGGTCATCTGATTGGAGA+AGG + chr8_2:64808997-64809016 None:intergenic 50.0%
!! TCATGGTGCCATCTGGATCA+TGG + chr8_2:64809275-64809294 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 64808665 64809422 64808665 ID=Msa1262190;Name=Msa1262190
chr8_2 mRNA 64808665 64809422 64808665 ID=Msa1262190-mRNA-1;Parent=Msa1262190;Name=Msa1262190-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0|0|1|1|1|3|0|167
chr8_2 exon 64809280 64809422 64809280 ID=Msa1262190-mRNA-1:exon:21004;Parent=Msa1262190-mRNA-1
chr8_2 exon 64809015 64809120 64809015 ID=Msa1262190-mRNA-1:exon:21003;Parent=Msa1262190-mRNA-1
chr8_2 exon 64808665 64808919 64808665 ID=Msa1262190-mRNA-1:exon:21002;Parent=Msa1262190-mRNA-1
chr8_2 CDS 64809280 64809422 64809280 ID=Msa1262190-mRNA-1:cds;Parent=Msa1262190-mRNA-1
chr8_2 CDS 64809015 64809120 64809015 ID=Msa1262190-mRNA-1:cds;Parent=Msa1262190-mRNA-1
chr8_2 CDS 64808665 64808919 64808665 ID=Msa1262190-mRNA-1:cds;Parent=Msa1262190-mRNA-1
Gene Sequence

>Msa1262190

ATGGGCAGTCCCCTTCAACTAAAATCATTGAATCACATTTCATTGGTGTGCAGATCATTAGACAAGTCAATTGATTTTTACGTCAATGTTCTTGGCTTCTTTCCTGTTAAAAGGCCTACCTCCTTAGCCTTCAATGGTGCATGGTTGTTTAATTATGGCATTGGCATACACCTTCTCCAATCAGATGACCCAGAAAGTATGACCAAGAATGTGCACATTAATCCAAAGGACAACCACATTTCTTTCCAATGTGAAAGTATGGTAGCAGTGGAGAATAAACTGCAGCAAATGAAGATAGAGTATGTGAAGAACAGAGTAGAGGAAAATGGAATCTACGTTGATCAGTTGTTCTTCCATGATCCAGATGGCACCATGATTGAAATCTGCAATTGTGACAATATTCCAGTTGTACCTCTATCAGAGAACACAATTTGGTCATGCTCGCGCTTCAACTGCAATATTCAAAATCAGCAGCAGCAAATTCATCAAATGATCTCAATGTAG

Protein sequence

>Msa1262190

MGSPLQLKSLNHISLVCRSLDKSIDFYVNVLGFFPVKRPTSLAFNGAWLFNYGIGIHLLQSDDPESMTKNVHINPKDNHISFQCESMVAVENKLQQMKIEYVKNRVEENGIYVDQLFFHDPDGTMIEICNCDNIPVVPLSENTIWSCSRFNCNIQNQQQQIHQMISM*