Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1422980 | G4XDS1 | 81.159 | 69 | 6 | 3 | 1 | 66 | 1 | 65 | 1.88e-23 | 102 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0615030 | Msa1422980 | 0.846589 | 1.974885e-59 | -8.615850e-47 |
| Msa0758250 | Msa1422980 | 0.800034 | 1.741728e-48 | -8.615850e-47 |
| Msa0836650 | Msa1422980 | 0.803607 | 3.215518e-49 | -8.615850e-47 |
| Msa0464440 | Msa1422980 | 0.801699 | 7.960468e-49 | -8.615850e-47 |
| Msa1095790 | Msa1422980 | 0.805199 | 1.497821e-49 | -8.615850e-47 |
| Msa1215070 | Msa1422980 | 0.809716 | 1.648841e-50 | -8.615850e-47 |
| Msa0559510 | Msa1422980 | 0.815117 | 1.089451e-51 | -8.615850e-47 |
| Msa0973280 | Msa1422980 | 0.807553 | 4.779552e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1422980 | MtrunA17_Chr7g0272591 | 94.030 | 67 | 2 | 2 | 1 | 66 | 1 | 66 | 6.50e-27 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAAATTTATCTTCTTTCA+TGG | 0.231855 | tig0025496:+62268 | None:intergenic |
| ATTAGGTGATGAATAAATTA+CGG | 0.358514 | tig0025496:-62305 | Msa1422980:intron |
| GAAACTAACGGACCGCTTAA+AGG | 0.384520 | tig0025496:+63248 | None:intergenic |
| ATCATCATCATCAAGAAATA+TGG | 0.391274 | tig0025496:-63334 | Msa1422980:CDS |
| CTCCACCTCATCGCTGAACC+TGG | 0.430406 | tig0025496:+63193 | None:intergenic |
| CAGGTTCAGCGATGAGGTGG+AGG | 0.523948 | tig0025496:-63192 | Msa1422980:CDS |
| CGCCAGGTTCAGCGATGAGG+TGG | 0.525195 | tig0025496:-63195 | Msa1422980:CDS |
| AGCACGAGAGTAGGCTTCAG+TGG | 0.544891 | tig0025496:-63284 | Msa1422980:CDS |
| GGAAGCAGAAGCACGAGAGT+AGG | 0.557076 | tig0025496:-63293 | Msa1422980:CDS |
| AAAGCAGCAAGAAAAGCGCC+AGG | 0.558816 | tig0025496:-63211 | Msa1422980:CDS |
| TCAAGAAATATGGTCGGAGA+CGG | 0.582861 | tig0025496:-63324 | Msa1422980:CDS |
| TGGTCGGAGACGGAGAGTAA+CGG | 0.584768 | tig0025496:-63314 | Msa1422980:CDS |
| TTCTTGTTGTTGGAAACTAA+CGG | 0.585089 | tig0025496:+63236 | None:intergenic |
| AAGCGCCAGGTTCAGCGATG+AGG | 0.642839 | tig0025496:-63198 | Msa1422980:CDS |
| TCATCATCAAGAAATATGGT+CGG | 0.656566 | tig0025496:-63330 | Msa1422980:CDS |
| GGCTTCAGTGGATCACTAAG+CGG | 0.669801 | tig0025496:-63272 | Msa1422980:CDS |
| TCACTAAGCGGACCTTTAAG+CGG | 0.676159 | tig0025496:-63260 | Msa1422980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTATTTTAATGAATTGTAA+AGG | + | tig0025496:62762-62781 | None:intergenic | 15.0% |
| !!! | TTATCGTAAAAATCATTTTA+TGG | + | tig0025496:62961-62980 | None:intergenic | 15.0% |
| !! | TGTGAATAATTTACTTAATA+CGG | - | tig0025496:63027-63046 | Msa1422980:intron | 15.0% |
| !! | GGTTAAACATATAAAAAAAA+AGG | - | tig0025496:63094-63113 | Msa1422980:intron | 15.0% |
| !! | AAGGAAATTATTAACATAAA+AGG | - | tig0025496:63113-63132 | Msa1422980:intron | 15.0% |
| !!! | GTTATTTTAATGAATTGTAA+AGG | + | tig0025496:62762-62781 | None:intergenic | 15.0% |
| !!! | TTATCGTAAAAATCATTTTA+TGG | + | tig0025496:62961-62980 | None:intergenic | 15.0% |
| !! | TGTGAATAATTTACTTAATA+CGG | - | tig0025496:63027-63046 | Msa1422980:intron | 15.0% |
| !! | GGTTAAACATATAAAAAAAA+AGG | - | tig0025496:63094-63113 | Msa1422980:intron | 15.0% |
| !! | AAGGAAATTATTAACATAAA+AGG | - | tig0025496:63113-63132 | Msa1422980:intron | 15.0% |
| !! | CACAAAAATAGTCATATTTA+TGG | - | tig0025496:62908-62927 | Msa1422980:intron | 20.0% |
| !! | TAATACGGTCAATAAAAATT+AGG | - | tig0025496:63042-63061 | Msa1422980:intron | 20.0% |
| !! | AATACGGTCAATAAAAATTA+GGG | - | tig0025496:63043-63062 | Msa1422980:intron | 20.0% |
| !! | TTACATTATATGATTGTGAT+CGG | + | tig0025496:63250-63269 | None:intergenic | 20.0% |
| !!! | TGACCTTGTTAATTTTTATT+AGG | - | tig0025496:63312-63331 | Msa1422980:CDS | 20.0% |
| !! | TCACCTAATAAAAATTAACA+AGG | + | tig0025496:63318-63337 | None:intergenic | 20.0% |
| !! | ATTAGGTGATGAATAAATTA+CGG | - | tig0025496:63329-63348 | Msa1422980:CDS | 20.0% |
| !! | CACAAAAATAGTCATATTTA+TGG | - | tig0025496:62908-62927 | Msa1422980:intron | 20.0% |
| !! | TAATACGGTCAATAAAAATT+AGG | - | tig0025496:63042-63061 | Msa1422980:intron | 20.0% |
| !! | AATACGGTCAATAAAAATTA+GGG | - | tig0025496:63043-63062 | Msa1422980:intron | 20.0% |
| !! | TTACATTATATGATTGTGAT+CGG | + | tig0025496:63250-63269 | None:intergenic | 20.0% |
| !!! | TGACCTTGTTAATTTTTATT+AGG | - | tig0025496:63312-63331 | Msa1422980:CDS | 20.0% |
| !! | TCACCTAATAAAAATTAACA+AGG | + | tig0025496:63318-63337 | None:intergenic | 20.0% |
| !! | ATTAGGTGATGAATAAATTA+CGG | - | tig0025496:63329-63348 | Msa1422980:CDS | 20.0% |
| ! | ATCATCATCATCAAGAAATA+TGG | - | tig0025496:62300-62319 | Msa1422980:CDS | 25.0% |
| !! | ATTTTACTCGTTTCCTAATA+TGG | + | tig0025496:62537-62556 | None:intergenic | 25.0% |
| ! | TATACAAGAAACTAATGCTA+TGG | - | tig0025496:62607-62626 | Msa1422980:intron | 25.0% |
| !!! | TATCATTTGACTTTTGTTTG+CGG | + | tig0025496:62854-62873 | None:intergenic | 25.0% |
| !! | TCGGCATGTAATTAATTTTT+AGG | + | tig0025496:63231-63250 | None:intergenic | 25.0% |
| ! | GTGGAATAAAAATTAACATG+TGG | + | tig0025496:63280-63299 | None:intergenic | 25.0% |
| ! | ATCATCATCATCAAGAAATA+TGG | - | tig0025496:62300-62319 | Msa1422980:CDS | 25.0% |
| !! | ATTTTACTCGTTTCCTAATA+TGG | + | tig0025496:62537-62556 | None:intergenic | 25.0% |
| ! | TATACAAGAAACTAATGCTA+TGG | - | tig0025496:62607-62626 | Msa1422980:intron | 25.0% |
| !!! | TATCATTTGACTTTTGTTTG+CGG | + | tig0025496:62854-62873 | None:intergenic | 25.0% |
| !! | TCGGCATGTAATTAATTTTT+AGG | + | tig0025496:63231-63250 | None:intergenic | 25.0% |
| ! | GTGGAATAAAAATTAACATG+TGG | + | tig0025496:63280-63299 | None:intergenic | 25.0% |
| TCATCATCAAGAAATATGGT+CGG | - | tig0025496:62304-62323 | Msa1422980:intron | 30.0% | |
| !! | TTCTTGTTGTTGGAAACTAA+CGG | + | tig0025496:62401-62420 | None:intergenic | 30.0% |
| AATGCTATGGTAAGCTATTA+TGG | - | tig0025496:62620-62639 | Msa1422980:intron | 30.0% | |
| ! | TCGCTGTGTAATAACTTTTA+CGG | - | tig0025496:62714-62733 | Msa1422980:intron | 30.0% |
| ! | TAAAGGTGTGTTAAAATGCA+TGG | + | tig0025496:62745-62764 | None:intergenic | 30.0% |
| !! | AAGAGAGAAGTATTACACAA+TGG | - | tig0025496:62808-62827 | Msa1422980:intron | 30.0% |
| AAATACTAACTGATGTCTCT+GGG | + | tig0025496:63207-63226 | None:intergenic | 30.0% | |
| AAAATACTAACTGATGTCTC+TGG | + | tig0025496:63208-63227 | None:intergenic | 30.0% | |
| AAGGTCAGTATATTTGAAAG+TGG | + | tig0025496:63299-63318 | None:intergenic | 30.0% | |
| TCATCATCAAGAAATATGGT+CGG | - | tig0025496:62304-62323 | Msa1422980:intron | 30.0% | |
| !! | TTCTTGTTGTTGGAAACTAA+CGG | + | tig0025496:62401-62420 | None:intergenic | 30.0% |
| AATGCTATGGTAAGCTATTA+TGG | - | tig0025496:62620-62639 | Msa1422980:intron | 30.0% | |
| ! | TCGCTGTGTAATAACTTTTA+CGG | - | tig0025496:62714-62733 | Msa1422980:intron | 30.0% |
| ! | TAAAGGTGTGTTAAAATGCA+TGG | + | tig0025496:62745-62764 | None:intergenic | 30.0% |
| !! | AAGAGAGAAGTATTACACAA+TGG | - | tig0025496:62808-62827 | Msa1422980:intron | 30.0% |
| AAATACTAACTGATGTCTCT+GGG | + | tig0025496:63207-63226 | None:intergenic | 30.0% | |
| AAAATACTAACTGATGTCTC+TGG | + | tig0025496:63208-63227 | None:intergenic | 30.0% | |
| AAGGTCAGTATATTTGAAAG+TGG | + | tig0025496:63299-63318 | None:intergenic | 30.0% | |
| ! | GAAGTATTACACAATGGTAG+AGG | - | tig0025496:62814-62833 | Msa1422980:intron | 35.0% |
| AAAATTAGGGTCATGCTAAC+CGG | - | tig0025496:63056-63075 | Msa1422980:intron | 35.0% | |
| ! | GAAGTATTACACAATGGTAG+AGG | - | tig0025496:62814-62833 | Msa1422980:intron | 35.0% |
| AAAATTAGGGTCATGCTAAC+CGG | - | tig0025496:63056-63075 | Msa1422980:intron | 35.0% | |
| TCAAGAAATATGGTCGGAGA+CGG | - | tig0025496:62310-62329 | Msa1422980:intron | 40.0% | |
| !!! | GCTGCTTTTCTTCTTGTTGT+TGG | + | tig0025496:62411-62430 | None:intergenic | 40.0% |
| TGCGCAAGTTATTGTAGCTA+GGG | + | tig0025496:62665-62684 | None:intergenic | 40.0% | |
| TCAAGAAATATGGTCGGAGA+CGG | - | tig0025496:62310-62329 | Msa1422980:intron | 40.0% | |
| !!! | GCTGCTTTTCTTCTTGTTGT+TGG | + | tig0025496:62411-62430 | None:intergenic | 40.0% |
| TGCGCAAGTTATTGTAGCTA+GGG | + | tig0025496:62665-62684 | None:intergenic | 40.0% | |
| TCACTAAGCGGACCTTTAAG+CGG | - | tig0025496:62374-62393 | Msa1422980:intron | 45.0% | |
| ! | GAAACTAACGGACCGCTTAA+AGG | + | tig0025496:62389-62408 | None:intergenic | 45.0% |
| ! | TCGAATGTTCTGGACCGAAA+CGG | + | tig0025496:62504-62523 | None:intergenic | 45.0% |
| CTGCGCAAGTTATTGTAGCT+AGG | + | tig0025496:62666-62685 | None:intergenic | 45.0% | |
| TACACAATGGTAGAGGCAAG+TGG | - | tig0025496:62821-62840 | Msa1422980:intron | 45.0% | |
| ACACAATGGTAGAGGCAAGT+GGG | - | tig0025496:62822-62841 | Msa1422980:intron | 45.0% | |
| ATATGTTTAACCAGTGCCGC+AGG | + | tig0025496:63086-63105 | None:intergenic | 45.0% | |
| !! | GATGTCTCTGGGACACATAT+AGG | + | tig0025496:63196-63215 | None:intergenic | 45.0% |
| TCACTAAGCGGACCTTTAAG+CGG | - | tig0025496:62374-62393 | Msa1422980:intron | 45.0% | |
| ! | GAAACTAACGGACCGCTTAA+AGG | + | tig0025496:62389-62408 | None:intergenic | 45.0% |
| ! | TCGAATGTTCTGGACCGAAA+CGG | + | tig0025496:62504-62523 | None:intergenic | 45.0% |
| CTGCGCAAGTTATTGTAGCT+AGG | + | tig0025496:62666-62685 | None:intergenic | 45.0% | |
| TACACAATGGTAGAGGCAAG+TGG | - | tig0025496:62821-62840 | Msa1422980:intron | 45.0% | |
| ACACAATGGTAGAGGCAAGT+GGG | - | tig0025496:62822-62841 | Msa1422980:intron | 45.0% | |
| ATATGTTTAACCAGTGCCGC+AGG | + | tig0025496:63086-63105 | None:intergenic | 45.0% | |
| !! | GATGTCTCTGGGACACATAT+AGG | + | tig0025496:63196-63215 | None:intergenic | 45.0% |
| GGCTTCAGTGGATCACTAAG+CGG | - | tig0025496:62362-62381 | Msa1422980:intron | 50.0% | |
| !! | AAAGCAGCAAGAAAAGCGCC+AGG | - | tig0025496:62423-62442 | Msa1422980:intron | 50.0% |
| GTTTCGGTCCAGAACATTCG+AGG | - | tig0025496:62503-62522 | Msa1422980:intron | 50.0% | |
| TATCACCGCCTCGAATGTTC+TGG | + | tig0025496:62514-62533 | None:intergenic | 50.0% | |
| CGAGGCGGTGATACCATATT+AGG | - | tig0025496:62521-62540 | Msa1422980:intron | 50.0% | |
| GGCTTCAGTGGATCACTAAG+CGG | - | tig0025496:62362-62381 | Msa1422980:intron | 50.0% | |
| !! | AAAGCAGCAAGAAAAGCGCC+AGG | - | tig0025496:62423-62442 | Msa1422980:intron | 50.0% |
| GTTTCGGTCCAGAACATTCG+AGG | - | tig0025496:62503-62522 | Msa1422980:intron | 50.0% | |
| TATCACCGCCTCGAATGTTC+TGG | + | tig0025496:62514-62533 | None:intergenic | 50.0% | |
| CGAGGCGGTGATACCATATT+AGG | - | tig0025496:62521-62540 | Msa1422980:intron | 50.0% | |
| TGGTCGGAGACGGAGAGTAA+CGG | - | tig0025496:62320-62339 | Msa1422980:intron | 55.0% | |
| ! | GGAAGCAGAAGCACGAGAGT+AGG | - | tig0025496:62341-62360 | Msa1422980:intron | 55.0% |
| AGCACGAGAGTAGGCTTCAG+TGG | - | tig0025496:62350-62369 | Msa1422980:intron | 55.0% | |
| CGATGAGGTGGAGGATTACG+TGG | - | tig0025496:62451-62470 | Msa1422980:intron | 55.0% | |
| CGTTCGCGATGACACCGTTT+CGG | - | tig0025496:62487-62506 | Msa1422980:intron | 55.0% | |
| TCGGTCCAGAACATTCGAGG+CGG | - | tig0025496:62506-62525 | Msa1422980:intron | 55.0% | |
| TGGTCGGAGACGGAGAGTAA+CGG | - | tig0025496:62320-62339 | Msa1422980:intron | 55.0% | |
| ! | GGAAGCAGAAGCACGAGAGT+AGG | - | tig0025496:62341-62360 | Msa1422980:intron | 55.0% |
| AGCACGAGAGTAGGCTTCAG+TGG | - | tig0025496:62350-62369 | Msa1422980:intron | 55.0% | |
| CGATGAGGTGGAGGATTACG+TGG | - | tig0025496:62451-62470 | Msa1422980:intron | 55.0% | |
| CGTTCGCGATGACACCGTTT+CGG | - | tig0025496:62487-62506 | Msa1422980:intron | 55.0% | |
| TCGGTCCAGAACATTCGAGG+CGG | - | tig0025496:62506-62525 | Msa1422980:intron | 55.0% | |
| AAGCGCCAGGTTCAGCGATG+AGG | - | tig0025496:62436-62455 | Msa1422980:intron | 60.0% | |
| CTCCACCTCATCGCTGAACC+TGG | + | tig0025496:62444-62463 | None:intergenic | 60.0% | |
| CAGGTTCAGCGATGAGGTGG+AGG | - | tig0025496:62442-62461 | Msa1422980:intron | 60.0% | |
| CATGCTAACCGGTGCTCCTG+CGG | - | tig0025496:63067-63086 | Msa1422980:intron | 60.0% | |
| AAGCGCCAGGTTCAGCGATG+AGG | - | tig0025496:62436-62455 | Msa1422980:intron | 60.0% | |
| CTCCACCTCATCGCTGAACC+TGG | + | tig0025496:62444-62463 | None:intergenic | 60.0% | |
| CAGGTTCAGCGATGAGGTGG+AGG | - | tig0025496:62442-62461 | Msa1422980:intron | 60.0% | |
| CATGCTAACCGGTGCTCCTG+CGG | - | tig0025496:63067-63086 | Msa1422980:intron | 60.0% | |
| CGCCAGGTTCAGCGATGAGG+TGG | - | tig0025496:62439-62458 | Msa1422980:intron | 65.0% | |
| ! | AACCAGTGCCGCAGGAGCAC+CGG | + | tig0025496:63078-63097 | None:intergenic | 65.0% |
| CGCCAGGTTCAGCGATGAGG+TGG | - | tig0025496:62439-62458 | Msa1422980:intron | 65.0% | |
| ! | AACCAGTGCCGCAGGAGCAC+CGG | + | tig0025496:63078-63097 | None:intergenic | 65.0% |
| AACCGGTGCTCCTGCGGCAC+TGG | - | tig0025496:63073-63092 | Msa1422980:intron | 70.0% | |
| AACCGGTGCTCCTGCGGCAC+TGG | - | tig0025496:63073-63092 | Msa1422980:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0025496 | gene | 62275 | 63381 | 62275 | ID=Msa1422980;Name=Msa1422980 |
| tig0025496 | mRNA | 62275 | 63381 | 62275 | ID=Msa1422980-mRNA-1;Parent=Msa1422980;Name=Msa1422980-mRNA-1;_AED=0.57;_eAED=0.58;_QI=0|0|0|0.5|0|0|2|0|80 |
| tig0025496 | exon | 63187 | 63381 | 63187 | ID=Msa1422980-mRNA-1:exon:2986;Parent=Msa1422980-mRNA-1 |
| tig0025496 | exon | 62275 | 62322 | 62275 | ID=Msa1422980-mRNA-1:exon:2985;Parent=Msa1422980-mRNA-1 |
| tig0025496 | CDS | 63187 | 63381 | 63187 | ID=Msa1422980-mRNA-1:cds;Parent=Msa1422980-mRNA-1 |
| tig0025496 | CDS | 62275 | 62322 | 62275 | ID=Msa1422980-mRNA-1:cds;Parent=Msa1422980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
