AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1215070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215070 MtrunA17_Chr4g0064141 98.496 133 2 0 46 178 1 133 1.24e-87 271
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215070 AT5G52580.2 69.853 136 35 4 46 178 3 135 5.83e-52 178
Msa1215070 AT5G52580.1 69.853 136 35 4 46 178 3 135 6.42e-52 177
Msa1215070 AT5G52580.3 66.901 142 35 5 46 178 3 141 1.53e-49 171

Find 40 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGGGACTTCCTTGTTTA+TGG 0.149479 8_1:+76147212 Msa1215070:CDS
CCAACTCAGTTTGCTATTAG+TGG 0.288769 8_1:+76147171 Msa1215070:CDS
ACAGAAACCTTTACACCATT+AGG 0.321041 8_1:+76147461 Msa1215070:CDS
TTACATGGTTGATACATATC+AGG 0.331972 8_1:-76147655 None:intergenic
CATTAGGGCGGTGCCGTTTA+CGG 0.353169 8_1:+76147477 Msa1215070:CDS
CAGAAACCTTTACACCATTA+GGG 0.356617 8_1:+76147462 Msa1215070:CDS
GGAGAGAAAGAACGCGCGTT+TGG 0.359846 8_1:-76146690 None:intergenic
AAAGGGCATAACGCGGATAA+TGG 0.370518 8_1:+76147334 Msa1215070:CDS
CGTAAACGGCACCGCCCTAA+TGG 0.383654 8_1:-76147476 None:intergenic
GATAATGGTTTGTCTGATAA+AGG 0.386252 8_1:+76147349 Msa1215070:CDS
ATCATTGTTGTTCTATCGTC+AGG 0.398946 8_1:+76147547 Msa1215070:CDS
AAGCTCATCTTCTAATGAAC+TGG 0.399668 8_1:+76147104 Msa1215070:CDS
CATTAGAAGATGAGCTTTGT+TGG 0.405449 8_1:-76147098 None:intergenic
ATGAATTGCAACATTATCCT+TGG 0.422003 8_1:-76147148 None:intergenic
CATTAATAACAGAACTTACA+TGG 0.431158 8_1:-76147670 None:intergenic
TCCGCGTTATGCCCTTTGTA+AGG 0.444284 8_1:-76147328 None:intergenic
AGGAGGCATACTCCTGCTCT+TGG 0.450918 8_1:+76147514 Msa1215070:CDS
ATTGACCTGACATCCGTAAA+CGG 0.471464 8_1:-76147490 None:intergenic
TTACGGATGTCAGGTCAATC+AGG 0.498746 8_1:+76147494 Msa1215070:CDS
CCACTAATAGCAAACTGAGT+TGG 0.524205 8_1:-76147171 None:intergenic
CGGTGCCGTTTACGGATGTC+AGG 0.524230 8_1:+76147485 Msa1215070:CDS
GAGATGTATCTTCGAGGCGA+AGG 0.534477 8_1:-76146737 None:intergenic
AGACTTGGATACCTTACAAA+GGG 0.544915 8_1:+76147317 Msa1215070:intron
ATATATTGCCATCCAAGAGC+AGG 0.548583 8_1:-76147526 None:intergenic
AGAAAATCTGTGATGTGAGA+AGG 0.554615 8_1:-76146711 None:intergenic
GATAGAGAGATGTATCTTCG+AGG 0.561619 8_1:-76146743 None:intergenic
GGCATACTCCTGCTCTTGGA+TGG 0.564573 8_1:+76147518 Msa1215070:CDS
GCACCGCCCTAATGGTGTAA+AGG 0.570683 8_1:-76147468 None:intergenic
TGCTGAAATTGTATATTCCA+AGG 0.571019 8_1:+76147131 Msa1215070:CDS
AGATTGAAGTTAATTAAGCA+AGG 0.571779 8_1:+76147195 Msa1215070:CDS
GATTGAAGTTAATTAAGCAA+GGG 0.611023 8_1:+76147196 Msa1215070:CDS
TCATCTTCTAATGAACTGGA+AGG 0.615316 8_1:+76147108 Msa1215070:CDS
CAGACTTGGATACCTTACAA+AGG 0.621609 8_1:+76147316 Msa1215070:intron
AGCAGCGTAATCGCTGTCGT+CGG 0.627659 8_1:-76146847 None:intergenic
TACATGGTTGATACATATCA+GGG 0.633605 8_1:-76147654 None:intergenic
CGTAATCGCTGTCGTCGGAG+AGG 0.638919 8_1:-76146842 None:intergenic
ATCGATTATCACCATAAACA+AGG 0.649905 8_1:-76147223 None:intergenic
CGGATGTCAGGTCAATCAGG+AGG 0.673482 8_1:+76147497 Msa1215070:CDS
ACCTTACAAAGGGCATAACG+CGG 0.687799 8_1:+76147327 Msa1215070:CDS
AAACCTTTACACCATTAGGG+CGG 0.720822 8_1:+76147465 Msa1215070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTCTTTTGTTAATTTT+CGG + chr8_1:76147245-76147264 Msa1215070:intron 10.0%
!!! TTAATTCTTTTGTTAATTTT+CGG + chr8_1:76147245-76147264 Msa1215070:intron 10.0%
!! AATCCAATTTCATTTCAATT+AGG + chr8_1:76146913-76146932 Msa1215070:intron 20.0%
!! ATCCAATTTCATTTCAATTA+GGG + chr8_1:76146914-76146933 Msa1215070:intron 20.0%
!! AATTTGAATTGTTTGTGATT+AGG + chr8_1:76147041-76147060 Msa1215070:intron 20.0%
!! ATTTGAATTGTTTGTGATTA+GGG + chr8_1:76147042-76147061 Msa1215070:intron 20.0%
!!! CTTTATTTTTCAGTTTATCA+TGG + chr8_1:76147619-76147638 Msa1215070:CDS 20.0%
!! AATCCAATTTCATTTCAATT+AGG + chr8_1:76146913-76146932 Msa1215070:intron 20.0%
!! ATCCAATTTCATTTCAATTA+GGG + chr8_1:76146914-76146933 Msa1215070:intron 20.0%
!! AATTTGAATTGTTTGTGATT+AGG + chr8_1:76147041-76147060 Msa1215070:intron 20.0%
!! ATTTGAATTGTTTGTGATTA+GGG + chr8_1:76147042-76147061 Msa1215070:intron 20.0%
!!! CTTTATTTTTCAGTTTATCA+TGG + chr8_1:76147619-76147638 Msa1215070:CDS 20.0%
!!! AATTGGATTTTGAAGTGAAA+AGG - chr8_1:76146902-76146921 None:intergenic 25.0%
! AACCCTAATTGAAATGAAAT+TGG - chr8_1:76146919-76146938 None:intergenic 25.0%
! AGATTTGAATTGAATTGAAC+CGG - chr8_1:76146966-76146985 None:intergenic 25.0%
!! CACTACTTTGTTTGTATTAT+TGG + chr8_1:76146995-76147014 Msa1215070:intron 25.0%
! AGATTGAAGTTAATTAAGCA+AGG + chr8_1:76147195-76147214 Msa1215070:CDS 25.0%
! GATTGAAGTTAATTAAGCAA+GGG + chr8_1:76147196-76147215 Msa1215070:CDS 25.0%
! AACAAAAAAACTTCAACACT+TGG - chr8_1:76147427-76147446 None:intergenic 25.0%
!!! AATTGGATTTTGAAGTGAAA+AGG - chr8_1:76146902-76146921 None:intergenic 25.0%
! AACCCTAATTGAAATGAAAT+TGG - chr8_1:76146919-76146938 None:intergenic 25.0%
! AGATTTGAATTGAATTGAAC+CGG - chr8_1:76146966-76146985 None:intergenic 25.0%
!! CACTACTTTGTTTGTATTAT+TGG + chr8_1:76146995-76147014 Msa1215070:intron 25.0%
! AGATTGAAGTTAATTAAGCA+AGG + chr8_1:76147195-76147214 Msa1215070:CDS 25.0%
! GATTGAAGTTAATTAAGCAA+GGG + chr8_1:76147196-76147215 Msa1215070:CDS 25.0%
! AACAAAAAAACTTCAACACT+TGG - chr8_1:76147427-76147446 None:intergenic 25.0%
ATTGTGATTGTGATTCAATC+CGG + chr8_1:76146944-76146963 Msa1215070:intron 30.0%
TGCTGAAATTGTATATTCCA+AGG + chr8_1:76147131-76147150 Msa1215070:CDS 30.0%
ATGAATTGCAACATTATCCT+TGG - chr8_1:76147151-76147170 None:intergenic 30.0%
ATCGATTATCACCATAAACA+AGG - chr8_1:76147226-76147245 None:intergenic 30.0%
! GATAATGGTTTGTCTGATAA+AGG + chr8_1:76147349-76147368 Msa1215070:CDS 30.0%
! AAGGTAGTTTGCATTTATAG+TGG + chr8_1:76147368-76147387 Msa1215070:intron 30.0%
! TACATGGTTGATACATATCA+GGG - chr8_1:76147657-76147676 None:intergenic 30.0%
! TTACATGGTTGATACATATC+AGG - chr8_1:76147658-76147677 None:intergenic 30.0%
ATTGTGATTGTGATTCAATC+CGG + chr8_1:76146944-76146963 Msa1215070:intron 30.0%
TGCTGAAATTGTATATTCCA+AGG + chr8_1:76147131-76147150 Msa1215070:CDS 30.0%
ATGAATTGCAACATTATCCT+TGG - chr8_1:76147151-76147170 None:intergenic 30.0%
ATCGATTATCACCATAAACA+AGG - chr8_1:76147226-76147245 None:intergenic 30.0%
! GATAATGGTTTGTCTGATAA+AGG + chr8_1:76147349-76147368 Msa1215070:CDS 30.0%
! AAGGTAGTTTGCATTTATAG+TGG + chr8_1:76147368-76147387 Msa1215070:intron 30.0%
! TACATGGTTGATACATATCA+GGG - chr8_1:76147657-76147676 None:intergenic 30.0%
! TTACATGGTTGATACATATC+AGG - chr8_1:76147658-76147677 None:intergenic 30.0%
AGAAAATCTGTGATGTGAGA+AGG - chr8_1:76146714-76146733 None:intergenic 35.0%
! CATTAGAAGATGAGCTTTGT+TGG - chr8_1:76147101-76147120 None:intergenic 35.0%
AAGCTCATCTTCTAATGAAC+TGG + chr8_1:76147104-76147123 Msa1215070:CDS 35.0%
TCATCTTCTAATGAACTGGA+AGG + chr8_1:76147108-76147127 Msa1215070:CDS 35.0%
! ATTATTTTCGCTTGCAGACT+TGG + chr8_1:76147302-76147321 Msa1215070:intron 35.0%
AGACTTGGATACCTTACAAA+GGG + chr8_1:76147317-76147336 Msa1215070:intron 35.0%
TTTACTGCTCTGTGAAATTC+AGG + chr8_1:76147401-76147420 Msa1215070:intron 35.0%
ACAGAAACCTTTACACCATT+AGG + chr8_1:76147461-76147480 Msa1215070:CDS 35.0%
CAGAAACCTTTACACCATTA+GGG + chr8_1:76147462-76147481 Msa1215070:CDS 35.0%
! ATCATTGTTGTTCTATCGTC+AGG + chr8_1:76147547-76147566 Msa1215070:CDS 35.0%
AGAAAATCTGTGATGTGAGA+AGG - chr8_1:76146714-76146733 None:intergenic 35.0%
! CATTAGAAGATGAGCTTTGT+TGG - chr8_1:76147101-76147120 None:intergenic 35.0%
AAGCTCATCTTCTAATGAAC+TGG + chr8_1:76147104-76147123 Msa1215070:CDS 35.0%
TCATCTTCTAATGAACTGGA+AGG + chr8_1:76147108-76147127 Msa1215070:CDS 35.0%
! ATTATTTTCGCTTGCAGACT+TGG + chr8_1:76147302-76147321 Msa1215070:intron 35.0%
AGACTTGGATACCTTACAAA+GGG + chr8_1:76147317-76147336 Msa1215070:intron 35.0%
TTTACTGCTCTGTGAAATTC+AGG + chr8_1:76147401-76147420 Msa1215070:intron 35.0%
ACAGAAACCTTTACACCATT+AGG + chr8_1:76147461-76147480 Msa1215070:CDS 35.0%
CAGAAACCTTTACACCATTA+GGG + chr8_1:76147462-76147481 Msa1215070:CDS 35.0%
! ATCATTGTTGTTCTATCGTC+AGG + chr8_1:76147547-76147566 Msa1215070:CDS 35.0%
GATAGAGAGATGTATCTTCG+AGG - chr8_1:76146746-76146765 None:intergenic 40.0%
CCACTAATAGCAAACTGAGT+TGG - chr8_1:76147174-76147193 None:intergenic 40.0%
!! CCAACTCAGTTTGCTATTAG+TGG + chr8_1:76147171-76147190 Msa1215070:CDS 40.0%
CAGACTTGGATACCTTACAA+AGG + chr8_1:76147316-76147335 Msa1215070:intron 40.0%
AAACCTTTACACCATTAGGG+CGG + chr8_1:76147465-76147484 Msa1215070:CDS 40.0%
ATTGACCTGACATCCGTAAA+CGG - chr8_1:76147493-76147512 None:intergenic 40.0%
ATATATTGCCATCCAAGAGC+AGG - chr8_1:76147529-76147548 None:intergenic 40.0%
GATAGAGAGATGTATCTTCG+AGG - chr8_1:76146746-76146765 None:intergenic 40.0%
CCACTAATAGCAAACTGAGT+TGG - chr8_1:76147174-76147193 None:intergenic 40.0%
!! CCAACTCAGTTTGCTATTAG+TGG + chr8_1:76147171-76147190 Msa1215070:CDS 40.0%
CAGACTTGGATACCTTACAA+AGG + chr8_1:76147316-76147335 Msa1215070:intron 40.0%
AAACCTTTACACCATTAGGG+CGG + chr8_1:76147465-76147484 Msa1215070:CDS 40.0%
ATTGACCTGACATCCGTAAA+CGG - chr8_1:76147493-76147512 None:intergenic 40.0%
ATATATTGCCATCCAAGAGC+AGG - chr8_1:76147529-76147548 None:intergenic 40.0%
! GCAAGGGACTTCCTTGTTTA+TGG + chr8_1:76147212-76147231 Msa1215070:CDS 45.0%
! ACCTTACAAAGGGCATAACG+CGG + chr8_1:76147327-76147346 Msa1215070:CDS 45.0%
! AAAGGGCATAACGCGGATAA+TGG + chr8_1:76147334-76147353 Msa1215070:CDS 45.0%
TTACGGATGTCAGGTCAATC+AGG + chr8_1:76147494-76147513 Msa1215070:CDS 45.0%
! GCAAGGGACTTCCTTGTTTA+TGG + chr8_1:76147212-76147231 Msa1215070:CDS 45.0%
! ACCTTACAAAGGGCATAACG+CGG + chr8_1:76147327-76147346 Msa1215070:CDS 45.0%
! AAAGGGCATAACGCGGATAA+TGG + chr8_1:76147334-76147353 Msa1215070:CDS 45.0%
TTACGGATGTCAGGTCAATC+AGG + chr8_1:76147494-76147513 Msa1215070:CDS 45.0%
GAGATGTATCTTCGAGGCGA+AGG - chr8_1:76146740-76146759 None:intergenic 50.0%
TCCGCGTTATGCCCTTTGTA+AGG - chr8_1:76147331-76147350 None:intergenic 50.0%
GAGATGTATCTTCGAGGCGA+AGG - chr8_1:76146740-76146759 None:intergenic 50.0%
TCCGCGTTATGCCCTTTGTA+AGG - chr8_1:76147331-76147350 None:intergenic 50.0%
GGAGAGAAAGAACGCGCGTT+TGG - chr8_1:76146693-76146712 None:intergenic 55.0%
AGCAGCGTAATCGCTGTCGT+CGG - chr8_1:76146850-76146869 None:intergenic 55.0%
GCACCGCCCTAATGGTGTAA+AGG - chr8_1:76147471-76147490 None:intergenic 55.0%
!! CATTAGGGCGGTGCCGTTTA+CGG + chr8_1:76147477-76147496 Msa1215070:CDS 55.0%
CGGATGTCAGGTCAATCAGG+AGG + chr8_1:76147497-76147516 Msa1215070:CDS 55.0%
! AGGAGGCATACTCCTGCTCT+TGG + chr8_1:76147514-76147533 Msa1215070:CDS 55.0%
GGCATACTCCTGCTCTTGGA+TGG + chr8_1:76147518-76147537 Msa1215070:CDS 55.0%
GGAGAGAAAGAACGCGCGTT+TGG - chr8_1:76146693-76146712 None:intergenic 55.0%
AGCAGCGTAATCGCTGTCGT+CGG - chr8_1:76146850-76146869 None:intergenic 55.0%
GCACCGCCCTAATGGTGTAA+AGG - chr8_1:76147471-76147490 None:intergenic 55.0%
!! CATTAGGGCGGTGCCGTTTA+CGG + chr8_1:76147477-76147496 Msa1215070:CDS 55.0%
CGGATGTCAGGTCAATCAGG+AGG + chr8_1:76147497-76147516 Msa1215070:CDS 55.0%
! AGGAGGCATACTCCTGCTCT+TGG + chr8_1:76147514-76147533 Msa1215070:CDS 55.0%
GGCATACTCCTGCTCTTGGA+TGG + chr8_1:76147518-76147537 Msa1215070:CDS 55.0%
CGTAATCGCTGTCGTCGGAG+AGG - chr8_1:76146845-76146864 None:intergenic 60.0%
CGTAAACGGCACCGCCCTAA+TGG - chr8_1:76147479-76147498 None:intergenic 60.0%
!! CGGTGCCGTTTACGGATGTC+AGG + chr8_1:76147485-76147504 Msa1215070:CDS 60.0%
CGTAATCGCTGTCGTCGGAG+AGG - chr8_1:76146845-76146864 None:intergenic 60.0%
CGTAAACGGCACCGCCCTAA+TGG - chr8_1:76147479-76147498 None:intergenic 60.0%
!! CGGTGCCGTTTACGGATGTC+AGG + chr8_1:76147485-76147504 Msa1215070:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 76146684 76147687 76146684 ID=Msa1215070;Name=Msa1215070
chr8_1 mRNA 76146684 76147687 76146684 ID=Msa1215070-mRNA-1;Parent=Msa1215070;Name=Msa1215070-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|1|1|1|1|4|0|217
chr8_1 exon 76146684 76146884 76146684 ID=Msa1215070-mRNA-1:exon:26138;Parent=Msa1215070-mRNA-1
chr8_1 exon 76147063 76147233 76147063 ID=Msa1215070-mRNA-1:exon:26139;Parent=Msa1215070-mRNA-1
chr8_1 exon 76147319 76147370 76147319 ID=Msa1215070-mRNA-1:exon:26140;Parent=Msa1215070-mRNA-1
chr8_1 exon 76147461 76147687 76147461 ID=Msa1215070-mRNA-1:exon:26141;Parent=Msa1215070-mRNA-1
chr8_1 CDS 76146684 76146884 76146684 ID=Msa1215070-mRNA-1:cds;Parent=Msa1215070-mRNA-1
chr8_1 CDS 76147063 76147233 76147063 ID=Msa1215070-mRNA-1:cds;Parent=Msa1215070-mRNA-1
chr8_1 CDS 76147319 76147370 76147319 ID=Msa1215070-mRNA-1:cds;Parent=Msa1215070-mRNA-1
chr8_1 CDS 76147461 76147687 76147461 ID=Msa1215070-mRNA-1:cds;Parent=Msa1215070-mRNA-1
Gene Sequence

>Msa1215070

CGCAAACCAAACGCGCGTTCTTTCTCTCCTTCTCACATCACAGATTTTCTTTTCCTTCGCCTCGAAGATACATCTCTCTATCATAAACAAACAATAATCGATTTTGTAAATAATCGCTTACATACACAATCGCTGATGCTAGAATCTGAACTTCACGACCTCTCCGACGACAGCGATTACGCTGCTGCGTCACAGCAACAAGGTTCGACTAGCGTGATGCAGCGTAGCGACAGCTTCCAACAAAGCTCATCTTCTAATGAACTGGAAGGTGCTGAAATTGTATATTCCAAGGATAATGTTGCAATTCATCCAACTCAGTTTGCTATTAGTGGAAGATTGAAGTTAATTAAGCAAGGGACTTCCTTGTTTATGACTTGGATACCTTACAAAGGGCATAACGCGGATAATGGTTTGTCTGATAAAGACAGAAACCTTTACACCATTAGGGCGGTGCCGTTTACGGATGTCAGGTCAATCAGGAGGCATACTCCTGCTCTTGGATGGCAATATATCATTGTTGTTCTATCGTCAGGTGAATTTTCTGTTGTTGAATTGAATCAATTGCATTTTATATTTATTCTGCTTTATTTTTCAGTTTATCATGGTTTTGTTATCTTCCCTGATATGTATCAACCATGTAAGTTCTGTTAT

Protein sequence

>Msa1215070

RKPNARSFSPSHITDFLFLRLEDTSLYHKQTIIDFVNNRLHTQSLMLESELHDLSDDSDYAAASQQQGSTSVMQRSDSFQQSSSSNELEGAEIVYSKDNVAIHPTQFAISGRLKLIKQGTSLFMTWIPYKGHNADNGLSDKDRNLYTIRAVPFTDVRSIRRHTPALGWQYIIVVLSSGEFSVVELNQLHFIFILLYFSVYHGFVIFPDMYQPCKFCY