Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1424150 | A0A2Z6PEI7 | 90.476 | 42 | 4 | 0 | 19 | 60 | 407 | 448 | 7.98e-15 | 77.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1397860 | Msa1424150 | 0.843106 | 1.718399e-58 | -8.615850e-47 |
| Msa0686270 | Msa1424150 | 0.822312 | 2.534522e-53 | -8.615850e-47 |
| Msa0237750 | Msa1424150 | 0.819384 | 1.194817e-52 | -8.615850e-47 |
| Msa0402290 | Msa1424150 | 0.852294 | 5.073569e-61 | -8.615850e-47 |
| Msa0998680 | Msa1424150 | 0.913477 | 5.881867e-84 | -8.615850e-47 |
| Msa1073240 | Msa1424150 | 0.991834 | 8.486069e-190 | -8.615850e-47 |
| Msa0355220 | Msa1424150 | 0.844098 | 9.329646e-59 | -8.615850e-47 |
| Msa0493000 | Msa1424150 | 0.806926 | 6.487360e-50 | -8.615850e-47 |
| Msa0598780 | Msa1424150 | 0.802486 | 5.483045e-49 | -8.615850e-47 |
| Msa0598790 | Msa1424150 | 0.927088 | 1.911961e-91 | -8.615850e-47 |
| Msa0967280 | Msa1424150 | 0.806425 | 8.276832e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1424150 | MtrunA17_Chr1g0186411 | 97.143 | 35 | 1 | 0 | 19 | 53 | 402 | 436 | 4.51e-16 | 70.9 |
| Msa1424150 | MtrunA17_Chr1g0168221 | 80.000 | 35 | 7 | 0 | 19 | 53 | 406 | 440 | 3.03e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTCACGTGAGATTCAAAA+TGG | 0.279377 | tig0025631:+56506 | None:intergenic |
| ATGGCTAAATTATTCGTATA+AGG | 0.295433 | tig0025631:-55820 | Msa1424150:three_prime_UTR |
| AGTTAGTACCATCTTGGAAT+TGG | 0.364864 | tig0025631:+55744 | None:intergenic |
| GCCATGATGCCTCTTCTTCA+CGG | 0.372818 | tig0025631:+56100 | None:intergenic |
| TTCCCAGCTGATTAGAGTTA+TGG | 0.402928 | tig0025631:-55839 | Msa1424150:exon |
| TAGCCATAACTCTAATCAGC+TGG | 0.446311 | tig0025631:+55836 | None:intergenic |
| ACTCACGTGAGATTCAAAAT+GGG | 0.510554 | tig0025631:+56507 | None:intergenic |
| ACCATCTTGGAATTGGACTT+AGG | 0.515604 | tig0025631:+55751 | None:intergenic |
| GGGTAGAGTTAGTACCATCT+TGG | 0.533104 | tig0025631:+55738 | None:intergenic |
| CAAAACGTGGAGTCAATGAG+AGG | 0.543462 | tig0025631:-56056 | Msa1424150:CDS |
| AAAACTAGTTTAAGAGGTGA+AGG | 0.552582 | tig0025631:+55789 | None:intergenic |
| AGAACTCATCCGTGAAGAAG+AGG | 0.564259 | tig0025631:-56109 | Msa1424150:CDS |
| AGTCAATGAGAGGGAAAAGA+AGG | 0.577856 | tig0025631:-56046 | Msa1424150:CDS |
| TCCGTGAAGAAGAGGCATCA+TGG | 0.580342 | tig0025631:-56101 | Msa1424150:CDS |
| GAGGCATCATGGCAAGCTGA+AGG | 0.593174 | tig0025631:-56090 | Msa1424150:CDS |
| AGCCATAACTCTAATCAGCT+GGG | 0.594139 | tig0025631:+55837 | None:intergenic |
| ACCTAAGTCCAATTCCAAGA+TGG | 0.606889 | tig0025631:-55752 | Msa1424150:three_prime_UTR |
| CTCACGTGAGATTCAAAATG+GGG | 0.622186 | tig0025631:+56508 | None:intergenic |
| AAAACGTGGAGTCAATGAGA+GGG | 0.644020 | tig0025631:-56055 | Msa1424150:CDS |
| GGTGATCAGAAAACAAAACG+TGG | 0.704649 | tig0025631:-56069 | Msa1424150:CDS |
| TCACGTGAGATTCAAAATGG+GGG | 0.709510 | tig0025631:+56509 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATTTTTTATTATTTTTT+TGG | - | tig0025631:56051-56070 | Msa1424150:CDS | 0.0% |
| !!! | TTAATTTTTTATTATTTTTT+TGG | - | tig0025631:56051-56070 | Msa1424150:CDS | 0.0% |
| !!! | AAGATGAAATTTAAGTTAAT+GGG | + | tig0025631:55879-55898 | None:intergenic | 15.0% |
| !!! | AAGATGAAATTTAAGTTAAT+GGG | + | tig0025631:55879-55898 | None:intergenic | 15.0% |
| !!! | TTCTGAAAATATGAAGAAAA+TGG | + | tig0025631:55834-55853 | None:intergenic | 20.0% |
| !!! | GAAGATGAAATTTAAGTTAA+TGG | + | tig0025631:55880-55899 | None:intergenic | 20.0% |
| !!! | TATTTTCAGCAAAAAAATTG+AGG | - | tig0025631:56092-56111 | Msa1424150:CDS | 20.0% |
| !!! | TTCTGAAAATATGAAGAAAA+TGG | + | tig0025631:55834-55853 | None:intergenic | 20.0% |
| !!! | GAAGATGAAATTTAAGTTAA+TGG | + | tig0025631:55880-55899 | None:intergenic | 20.0% |
| !!! | TATTTTCAGCAAAAAAATTG+AGG | - | tig0025631:56092-56111 | Msa1424150:CDS | 20.0% |
| ! | TGAAAATATGAAGAAAATGG+TGG | + | tig0025631:55831-55850 | None:intergenic | 25.0% |
| !! | TGAAATTTAAGTTAATGGGT+AGG | + | tig0025631:55875-55894 | None:intergenic | 25.0% |
| ! | TTTCACTTTCCATTAAAGTT+TGG | - | tig0025631:55923-55942 | Msa1424150:intron | 25.0% |
| !!! | TCTGAAAACTTTTAACTTGA+AGG | - | tig0025631:56002-56021 | Msa1424150:intron | 25.0% |
| ! | AGATTGAAATTGCATCATTA+AGG | - | tig0025631:56262-56281 | Msa1424150:intron | 25.0% |
| ! | ATGGCTAAATTATTCGTATA+AGG | - | tig0025631:56441-56460 | Msa1424150:intron | 25.0% |
| ! | TGAAAATATGAAGAAAATGG+TGG | + | tig0025631:55831-55850 | None:intergenic | 25.0% |
| !! | TGAAATTTAAGTTAATGGGT+AGG | + | tig0025631:55875-55894 | None:intergenic | 25.0% |
| ! | TTTCACTTTCCATTAAAGTT+TGG | - | tig0025631:55923-55942 | Msa1424150:intron | 25.0% |
| !!! | TCTGAAAACTTTTAACTTGA+AGG | - | tig0025631:56002-56021 | Msa1424150:intron | 25.0% |
| ! | AGATTGAAATTGCATCATTA+AGG | - | tig0025631:56262-56281 | Msa1424150:intron | 25.0% |
| ! | ATGGCTAAATTATTCGTATA+AGG | - | tig0025631:56441-56460 | Msa1424150:intron | 25.0% |
| TTGCAATCTCCAAACTTTAA+TGG | + | tig0025631:55935-55954 | None:intergenic | 30.0% | |
| AAAAATTGAGGTTGCTTAAC+AGG | - | tig0025631:56104-56123 | Msa1424150:CDS | 30.0% | |
| ! | AAAACTAGTTTAAGAGGTGA+AGG | + | tig0025631:56475-56494 | None:intergenic | 30.0% |
| !!! | TCACCTCTTAAACTAGTTTT+TGG | - | tig0025631:56475-56494 | Msa1424150:intron | 30.0% |
| ! | ACTCCAAAAACTAGTTTAAG+AGG | + | tig0025631:56481-56500 | None:intergenic | 30.0% |
| !!! | TAAACTAGTTTTTGGAGTTG+AGG | - | tig0025631:56483-56502 | Msa1424150:intron | 30.0% |
| !!! | TCTACCCTAGTTTTGTTTTT+GGG | - | tig0025631:56539-56558 | Msa1424150:CDS | 30.0% |
| TTGCAATCTCCAAACTTTAA+TGG | + | tig0025631:55935-55954 | None:intergenic | 30.0% | |
| AAAAATTGAGGTTGCTTAAC+AGG | - | tig0025631:56104-56123 | Msa1424150:CDS | 30.0% | |
| ! | AAAACTAGTTTAAGAGGTGA+AGG | + | tig0025631:56475-56494 | None:intergenic | 30.0% |
| !!! | TCACCTCTTAAACTAGTTTT+TGG | - | tig0025631:56475-56494 | Msa1424150:intron | 30.0% |
| ! | ACTCCAAAAACTAGTTTAAG+AGG | + | tig0025631:56481-56500 | None:intergenic | 30.0% |
| !!! | TAAACTAGTTTTTGGAGTTG+AGG | - | tig0025631:56483-56502 | Msa1424150:intron | 30.0% |
| !!! | TCTACCCTAGTTTTGTTTTT+GGG | - | tig0025631:56539-56558 | Msa1424150:CDS | 30.0% |
| ACTCACGTGAGATTCAAAAT+GGG | + | tig0025631:55757-55776 | None:intergenic | 35.0% | |
| TCTTTGTGAGATTCAAGTGA+AGG | + | tig0025631:55794-55813 | None:intergenic | 35.0% | |
| TTGGAGATTGCAACTTAGTT+TGG | - | tig0025631:55942-55961 | Msa1424150:intron | 35.0% | |
| AAAGAAGGCAAAGAAAAAGC+AGG | - | tig0025631:56230-56249 | Msa1424150:intron | 35.0% | |
| AGTTAGTACCATCTTGGAAT+TGG | + | tig0025631:56520-56539 | None:intergenic | 35.0% | |
| !!! | CTCTACCCTAGTTTTGTTTT+TGG | - | tig0025631:56538-56557 | Msa1424150:CDS | 35.0% |
| TTGGCCCAAAAACAAAACTA+GGG | + | tig0025631:56546-56565 | None:intergenic | 35.0% | |
| ACTCACGTGAGATTCAAAAT+GGG | + | tig0025631:55757-55776 | None:intergenic | 35.0% | |
| TCTTTGTGAGATTCAAGTGA+AGG | + | tig0025631:55794-55813 | None:intergenic | 35.0% | |
| TTGGAGATTGCAACTTAGTT+TGG | - | tig0025631:55942-55961 | Msa1424150:intron | 35.0% | |
| AAAGAAGGCAAAGAAAAAGC+AGG | - | tig0025631:56230-56249 | Msa1424150:intron | 35.0% | |
| AGTTAGTACCATCTTGGAAT+TGG | + | tig0025631:56520-56539 | None:intergenic | 35.0% | |
| !!! | CTCTACCCTAGTTTTGTTTT+TGG | - | tig0025631:56538-56557 | Msa1424150:CDS | 35.0% |
| TTGGCCCAAAAACAAAACTA+GGG | + | tig0025631:56546-56565 | None:intergenic | 35.0% | |
| AGATTCAAAATGGGGGTTGA+TGG | + | tig0025631:55748-55767 | None:intergenic | 40.0% | |
| TCACGTGAGATTCAAAATGG+GGG | + | tig0025631:55755-55774 | None:intergenic | 40.0% | |
| CTCACGTGAGATTCAAAATG+GGG | + | tig0025631:55756-55775 | None:intergenic | 40.0% | |
| GACTCACGTGAGATTCAAAA+TGG | + | tig0025631:55758-55777 | None:intergenic | 40.0% | |
| GGTGATCAGAAAACAAAACG+TGG | - | tig0025631:56192-56211 | Msa1424150:intron | 40.0% | |
| AAAACGTGGAGTCAATGAGA+GGG | - | tig0025631:56206-56225 | Msa1424150:intron | 40.0% | |
| AGTCAATGAGAGGGAAAAGA+AGG | - | tig0025631:56215-56234 | Msa1424150:intron | 40.0% | |
| TTCCCAGCTGATTAGAGTTA+TGG | - | tig0025631:56422-56441 | Msa1424150:intron | 40.0% | |
| AGCCATAACTCTAATCAGCT+GGG | + | tig0025631:56427-56446 | None:intergenic | 40.0% | |
| TAGCCATAACTCTAATCAGC+TGG | + | tig0025631:56428-56447 | None:intergenic | 40.0% | |
| ACCTAAGTCCAATTCCAAGA+TGG | - | tig0025631:56509-56528 | Msa1424150:intron | 40.0% | |
| ACCATCTTGGAATTGGACTT+AGG | + | tig0025631:56513-56532 | None:intergenic | 40.0% | |
| CTTGGCCCAAAAACAAAACT+AGG | + | tig0025631:56547-56566 | None:intergenic | 40.0% | |
| AGATTCAAAATGGGGGTTGA+TGG | + | tig0025631:55748-55767 | None:intergenic | 40.0% | |
| TCACGTGAGATTCAAAATGG+GGG | + | tig0025631:55755-55774 | None:intergenic | 40.0% | |
| CTCACGTGAGATTCAAAATG+GGG | + | tig0025631:55756-55775 | None:intergenic | 40.0% | |
| GACTCACGTGAGATTCAAAA+TGG | + | tig0025631:55758-55777 | None:intergenic | 40.0% | |
| GGTGATCAGAAAACAAAACG+TGG | - | tig0025631:56192-56211 | Msa1424150:intron | 40.0% | |
| AAAACGTGGAGTCAATGAGA+GGG | - | tig0025631:56206-56225 | Msa1424150:intron | 40.0% | |
| AGTCAATGAGAGGGAAAAGA+AGG | - | tig0025631:56215-56234 | Msa1424150:intron | 40.0% | |
| TTCCCAGCTGATTAGAGTTA+TGG | - | tig0025631:56422-56441 | Msa1424150:intron | 40.0% | |
| AGCCATAACTCTAATCAGCT+GGG | + | tig0025631:56427-56446 | None:intergenic | 40.0% | |
| TAGCCATAACTCTAATCAGC+TGG | + | tig0025631:56428-56447 | None:intergenic | 40.0% | |
| ACCTAAGTCCAATTCCAAGA+TGG | - | tig0025631:56509-56528 | Msa1424150:intron | 40.0% | |
| ACCATCTTGGAATTGGACTT+AGG | + | tig0025631:56513-56532 | None:intergenic | 40.0% | |
| CTTGGCCCAAAAACAAAACT+AGG | + | tig0025631:56547-56566 | None:intergenic | 40.0% | |
| AGAACTCATCCGTGAAGAAG+AGG | - | tig0025631:56152-56171 | Msa1424150:intron | 45.0% | |
| CAAAACGTGGAGTCAATGAG+AGG | - | tig0025631:56205-56224 | Msa1424150:intron | 45.0% | |
| GGGTAGAGTTAGTACCATCT+TGG | + | tig0025631:56526-56545 | None:intergenic | 45.0% | |
| AGAACTCATCCGTGAAGAAG+AGG | - | tig0025631:56152-56171 | Msa1424150:intron | 45.0% | |
| CAAAACGTGGAGTCAATGAG+AGG | - | tig0025631:56205-56224 | Msa1424150:intron | 45.0% | |
| GGGTAGAGTTAGTACCATCT+TGG | + | tig0025631:56526-56545 | None:intergenic | 45.0% | |
| !! | TCCGTGAAGAAGAGGCATCA+TGG | - | tig0025631:56160-56179 | Msa1424150:intron | 50.0% |
| GCCATGATGCCTCTTCTTCA+CGG | + | tig0025631:56164-56183 | None:intergenic | 50.0% | |
| !! | TCCGTGAAGAAGAGGCATCA+TGG | - | tig0025631:56160-56179 | Msa1424150:intron | 50.0% |
| GCCATGATGCCTCTTCTTCA+CGG | + | tig0025631:56164-56183 | None:intergenic | 50.0% | |
| ! | GAGGCATCATGGCAAGCTGA+AGG | - | tig0025631:56171-56190 | Msa1424150:intron | 55.0% |
| ! | GAGGCATCATGGCAAGCTGA+AGG | - | tig0025631:56171-56190 | Msa1424150:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0025631 | gene | 55717 | 56566 | 55717 | ID=Msa1424150;Name=Msa1424150 |
| tig0025631 | mRNA | 55717 | 56566 | 55717 | ID=Msa1424150-mRNA-1;Parent=Msa1424150;Name=Msa1424150-mRNA-1;_AED=0.35;_eAED=0.48;_QI=0|0.5|0.33|0.66|1|1|3|134|77 |
| tig0025631 | exon | 55717 | 55904 | 55717 | ID=Msa1424150-mRNA-1:exon:3632;Parent=Msa1424150-mRNA-1 |
| tig0025631 | exon | 56032 | 56157 | 56032 | ID=Msa1424150-mRNA-1:exon:3631;Parent=Msa1424150-mRNA-1 |
| tig0025631 | exon | 56513 | 56566 | 56513 | ID=Msa1424150-mRNA-1:exon:3630;Parent=Msa1424150-mRNA-1 |
| tig0025631 | CDS | 56513 | 56566 | 56513 | ID=Msa1424150-mRNA-1:cds;Parent=Msa1424150-mRNA-1 |
| tig0025631 | CDS | 56032 | 56157 | 56032 | ID=Msa1424150-mRNA-1:cds;Parent=Msa1424150-mRNA-1 |
| tig0025631 | CDS | 55851 | 55904 | 55851 | ID=Msa1424150-mRNA-1:cds;Parent=Msa1424150-mRNA-1 |
| tig0025631 | three_prime_UTR | 55717 | 55850 | 55717 | ID=Msa1424150-mRNA-1:three_prime_utr;Parent=Msa1424150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
