AlfalfaGEDB | Msa1424150

M. sativa cultivar zhongmu-4 / Msa1424150

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424150	A0A2Z6PEI7	90.476	42	4	0	19	60	407	448	7.98e-15	77.8

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0237750	Msa1424150	0.819384	1.194817e-52	-8.615850e-47
Msa0355220	Msa1424150	0.844098	9.329646e-59	-8.615850e-47
Msa0402290	Msa1424150	0.852294	5.073569e-61	-8.615850e-47
Msa0493000	Msa1424150	0.806926	6.487360e-50	-8.615850e-47
Msa0598780	Msa1424150	0.802486	5.483045e-49	-8.615850e-47
Msa0598790	Msa1424150	0.927088	1.911961e-91	-8.615850e-47
Msa0686270	Msa1424150	0.822312	2.534522e-53	-8.615850e-47
Msa0967280	Msa1424150	0.806425	8.276832e-50	-8.615850e-47
Msa0998680	Msa1424150	0.913477	5.881867e-84	-8.615850e-47
Msa1073240	Msa1424150	0.991834	8.486069e-190	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1424150	MtrunA17_Chr1g0186411	97.143	35	1	0	19	53	402	436	4.51e-16	70.9
Msa1424150	MtrunA17_Chr1g0168221	80.000	35	7	0	19	53	406	440	3.03e-11	57.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 21 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTGGAGTCAATGAGA+GGG	0.644020	tig0025631:-56055	Msa1424150:CDS
AAAACTAGTTTAAGAGGTGA+AGG	0.552582	tig0025631:+55789	None:intergenic
ACCATCTTGGAATTGGACTT+AGG	0.515604	tig0025631:+55751	None:intergenic
ACCTAAGTCCAATTCCAAGA+TGG	0.606889	tig0025631:-55752	Msa1424150:three_prime_UTR
ACTCACGTGAGATTCAAAAT+GGG	0.510554	tig0025631:+56507	None:intergenic
AGAACTCATCCGTGAAGAAG+AGG	0.564259	tig0025631:-56109	Msa1424150:CDS
AGCCATAACTCTAATCAGCT+GGG	0.594139	tig0025631:+55837	None:intergenic
AGTCAATGAGAGGGAAAAGA+AGG	0.577856	tig0025631:-56046	Msa1424150:CDS
AGTTAGTACCATCTTGGAAT+TGG	0.364864	tig0025631:+55744	None:intergenic
ATGGCTAAATTATTCGTATA+AGG	0.295433	tig0025631:-55820	Msa1424150:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGCAATCTCCAAACTTTAA+TGG	+	tig0025631:55935-55954	None:intergenic	30.0%
AAAAATTGAGGTTGCTTAAC+AGG	-	tig0025631:56104-56123	Msa1424150:CDS	30.0%
TTGCAATCTCCAAACTTTAA+TGG	+	tig0025631:55935-55954	None:intergenic	30.0%
AAAAATTGAGGTTGCTTAAC+AGG	-	tig0025631:56104-56123	Msa1424150:CDS	30.0%
ACTCACGTGAGATTCAAAAT+GGG	+	tig0025631:55757-55776	None:intergenic	35.0%
TCTTTGTGAGATTCAAGTGA+AGG	+	tig0025631:55794-55813	None:intergenic	35.0%
TTGGAGATTGCAACTTAGTT+TGG	-	tig0025631:55942-55961	Msa1424150:intron	35.0%
AAAGAAGGCAAAGAAAAAGC+AGG	-	tig0025631:56230-56249	Msa1424150:intron	35.0%
AGTTAGTACCATCTTGGAAT+TGG	+	tig0025631:56520-56539	None:intergenic	35.0%
TTGGCCCAAAAACAAAACTA+GGG	+	tig0025631:56546-56565	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0025631	gene	55717	56566	55717	ID=Msa1424150;Name=Msa1424150
tig0025631	mRNA	55717	56566	55717	ID=Msa1424150-mRNA-1;Parent=Msa1424150;Name=Msa1424150-mRNA-1;_AED=0.35;_eAED=0.48;_QI=0\|0.5\|0.33\|0.66\|1\|1\|3\|134\|77
tig0025631	exon	55717	55904	55717	ID=Msa1424150-mRNA-1:exon:3632;Parent=Msa1424150-mRNA-1
tig0025631	exon	56032	56157	56032	ID=Msa1424150-mRNA-1:exon:3631;Parent=Msa1424150-mRNA-1
tig0025631	exon	56513	56566	56513	ID=Msa1424150-mRNA-1:exon:3630;Parent=Msa1424150-mRNA-1
tig0025631	CDS	56513	56566	56513	ID=Msa1424150-mRNA-1:cds;Parent=Msa1424150-mRNA-1
tig0025631	CDS	56032	56157	56032	ID=Msa1424150-mRNA-1:cds;Parent=Msa1424150-mRNA-1
tig0025631	CDS	55851	55904	55851	ID=Msa1424150-mRNA-1:cds;Parent=Msa1424150-mRNA-1
tig0025631	three_prime_UTR	55717	55850	55717	ID=Msa1424150-mRNA-1:three_prime_utr;Parent=Msa1424150-mRNA-1

Gene Sequence	>Msa1424150 ATGCAGAGTAGCAATTCAAACTTACTAACCATCAACCCCCATTTTGAATCTCACGAAGTTGTTGCTCTAAAGAGACAAGAAGAACTCATCCGTGAAGAAGAGGCATCATGGCAAGCTGAAGGTGATCAGAAAACAAAACGTGGAGTCAATGAGAGGGAAAAGAAGGCAAAGAAAAAGCAGACTGAAATGCAATTGTCTGACAATTATATGAGATCTGTCGAGATTCCCAGCTGA
Protein sequence	>Msa1424150 MQSSNSNLLTINPHFESHEVVALKRQEELIREEEASWQAEGDQKTKRGVNEREKKAKKKQTEMQLSDNYMRSVEIPS*