AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686270 MtrunA17_Chr1g0186411 100.000 35 0 0 1 35 402 436 1.10e-16 71.6
Msa0686270 MtrunA17_Chr1g0168221 82.857 35 6 0 1 35 406 440 1.07e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAATAGCCACTCTTATT+CGG 0.242620 4_4:-50703134 None:intergenic
CTTTGGGCTGCTATATGCTT+TGG 0.295273 4_4:+50703404 Msa0686270:three_prime_UTR
ATGGCTAAATTATTCGTATA+AGG 0.295433 4_4:+50702523 Msa0686270:three_prime_UTR
TGAAGATATATGAAGATATT+AGG 0.315234 4_4:+50703306 Msa0686270:three_prime_UTR
AGTTAGTACCATCTTGGAAT+TGG 0.364864 4_4:-50702599 None:intergenic
AAAAGATGTATGAAGATATC+AGG 0.367738 4_4:+50703174 Msa0686270:three_prime_UTR
GCCATGATGCCTCTTCTTCA+CGG 0.372818 4_4:-50702243 None:intergenic
ATAAATAGCCACTCTTATTC+GGG 0.385024 4_4:-50703133 None:intergenic
TTCCCAGCTGATTAGAGTTA+TGG 0.402928 4_4:+50702504 Msa0686270:exon
TAGCCATAACTCTAATCAGC+TGG 0.446311 4_4:-50702507 None:intergenic
TTGCAAATTGATGTCTAAGC+TGG 0.453642 4_4:-50703056 None:intergenic
TGTCGATCACTTGAGTGCCT+TGG 0.466505 4_4:-50703083 None:intergenic
CTCAAGTGATCGACAAGACC+AGG 0.485596 4_4:+50703091 Msa0686270:three_prime_UTR
TTTCTACTAGTATACCCATC+AGG 0.511384 4_4:+50703010 Msa0686270:intron
ACCATCTTGGAATTGGACTT+AGG 0.530179 4_4:-50702592 None:intergenic
GGGTAGAGTTAGTACCATCT+TGG 0.533104 4_4:-50702605 None:intergenic
CAAAACGTGGAGTCAATGAG+AGG 0.543462 4_4:+50702287 Msa0686270:CDS
CGACAAGACCAGGGTTGTGA+AGG 0.552215 4_4:+50703101 Msa0686270:three_prime_UTR
AAAACTAGTTTAAGAGGTGA+AGG 0.552582 4_4:-50702554 None:intergenic
CGATCATAGACCTTCCTGAT+GGG 0.559470 4_4:-50703024 None:intergenic
AGAACTCATCCGTGAAGAAG+AGG 0.564259 4_4:+50702234 Msa0686270:CDS
AGTCAATGAGAGGGAAAAGA+AGG 0.577856 4_4:+50702297 Msa0686270:CDS
TCCGTGAAGAAGAGGCATCA+TGG 0.580342 4_4:+50702242 Msa0686270:CDS
GGTCAGTCGATCAAGAACCT+TGG 0.587010 4_4:-50703264 None:intergenic
TTGAAGATCCTTCACAACCC+TGG 0.590563 4_4:-50703109 None:intergenic
GAGGCATCATGGCAAGCTGA+AGG 0.593174 4_4:+50702253 Msa0686270:CDS
AGCCATAACTCTAATCAGCT+GGG 0.594139 4_4:-50702506 None:intergenic
TCGATCATAGACCTTCCTGA+TGG 0.594164 4_4:-50703025 None:intergenic
GAAGCACAGACAAATATGCT+TGG 0.598550 4_4:-50702644 None:intergenic
CGAACAAGTACAAGGAGTCA+AGG 0.618584 4_4:-50703481 None:intergenic
TGTACATGCAAGTTTAACCA+AGG 0.622831 4_4:+50703247 Msa0686270:three_prime_UTR
ACCTAAGTCCAATTCCAAGA+TGG 0.626598 4_4:+50702591 Msa0686270:three_prime_UTR
GCACAACTTACACTCAGGAC+TGG 0.628922 4_4:-50702672 None:intergenic
AAAACGTGGAGTCAATGAGA+GGG 0.644020 4_4:+50702288 Msa0686270:CDS
TACTAGTATACCCATCAGGA+AGG 0.654261 4_4:+50703014 Msa0686270:intron
TCTTCAATCCCGAATAAGAG+TGG 0.657199 4_4:+50703125 Msa0686270:three_prime_UTR
CATCAATTTGCAAAAGACCA+AGG 0.673397 4_4:+50703066 Msa0686270:three_prime_UTR
GGTGATCAGAAAACAAAACG+TGG 0.704649 4_4:+50702274 Msa0686270:CDS
TCAAGTGATCGACAAGACCA+GGG 0.710990 4_4:+50703092 Msa0686270:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATAATAAAGAATTAAATT+TGG - chr4_4:50702720-50702739 None:intergenic 10.0%
!! TCATAATAAAGAATTAAATT+TGG - chr4_4:50702720-50702739 None:intergenic 10.0%
!! TAATTCTTTATTATGATGTA+AGG + chr4_4:50702724-50702743 Msa0686270:intron 15.0%
!!! ATTTTAAAAGTGAAGATTTA+TGG + chr4_4:50703205-50703224 Msa0686270:three_prime_UTR 15.0%
!!! TATGTTCATAGTTTTTTTTA+GGG - chr4_4:50703455-50703474 None:intergenic 15.0%
!!! ATATGTTCATAGTTTTTTTT+AGG - chr4_4:50703456-50703475 None:intergenic 15.0%
!! AAAAAAACTATGAACATATA+TGG + chr4_4:50703457-50703476 Msa0686270:three_prime_UTR 15.0%
!! AAAAAAGAAATTTGTAAGAT+GGG + chr4_4:50703504-50703523 Msa0686270:three_prime_UTR 15.0%
!! TAATTCTTTATTATGATGTA+AGG + chr4_4:50702724-50702743 Msa0686270:intron 15.0%
!!! ATTTTAAAAGTGAAGATTTA+TGG + chr4_4:50703205-50703224 Msa0686270:three_prime_UTR 15.0%
!!! TATGTTCATAGTTTTTTTTA+GGG - chr4_4:50703455-50703474 None:intergenic 15.0%
!!! ATATGTTCATAGTTTTTTTT+AGG - chr4_4:50703456-50703475 None:intergenic 15.0%
!! AAAAAAACTATGAACATATA+TGG + chr4_4:50703457-50703476 Msa0686270:three_prime_UTR 15.0%
!! TGAAGATATATGAAGATATT+AGG + chr4_4:50703306-50703325 Msa0686270:three_prime_UTR 20.0%
!!! TAATGTTAATTTTGAACTAC+AGG + chr4_4:50703381-50703400 Msa0686270:three_prime_UTR 20.0%
!! GAAAAAAGAAATTTGTAAGA+TGG + chr4_4:50703503-50703522 Msa0686270:three_prime_UTR 20.0%
!! TGAAGATATATGAAGATATT+AGG + chr4_4:50703306-50703325 Msa0686270:three_prime_UTR 20.0%
!!! TAATGTTAATTTTGAACTAC+AGG + chr4_4:50703381-50703400 Msa0686270:three_prime_UTR 20.0%
! AGATTGAAATTGCATCATTA+AGG + chr4_4:50702344-50702363 Msa0686270:intron 25.0%
! ATGGCTAAATTATTCGTATA+AGG + chr4_4:50702523-50702542 Msa0686270:three_prime_UTR 25.0%
!! ATTGCAATGCATTTTGAATA+AGG + chr4_4:50702775-50702794 Msa0686270:intron 25.0%
! AACAAATCGAAAAACAAACA+TGG - chr4_4:50702836-50702855 None:intergenic 25.0%
! AATAAATAGCCACTCTTATT+CGG - chr4_4:50703137-50703156 None:intergenic 25.0%
! AAAAGATGTATGAAGATATC+AGG + chr4_4:50703174-50703193 Msa0686270:three_prime_UTR 25.0%
! ATTAGGTAAAAAAAGTGAAG+TGG + chr4_4:50703323-50703342 Msa0686270:three_prime_UTR 25.0%
! AGATTGAAATTGCATCATTA+AGG + chr4_4:50702344-50702363 Msa0686270:intron 25.0%
! ATGGCTAAATTATTCGTATA+AGG + chr4_4:50702523-50702542 Msa0686270:three_prime_UTR 25.0%
!! ATTGCAATGCATTTTGAATA+AGG + chr4_4:50702775-50702794 Msa0686270:intron 25.0%
! AACAAATCGAAAAACAAACA+TGG - chr4_4:50702836-50702855 None:intergenic 25.0%
! AATAAATAGCCACTCTTATT+CGG - chr4_4:50703137-50703156 None:intergenic 25.0%
! AAAAGATGTATGAAGATATC+AGG + chr4_4:50703174-50703193 Msa0686270:three_prime_UTR 25.0%
! ATTAGGTAAAAAAAGTGAAG+TGG + chr4_4:50703323-50703342 Msa0686270:three_prime_UTR 25.0%
! AAAACTAGTTTAAGAGGTGA+AGG - chr4_4:50702557-50702576 None:intergenic 30.0%
!!! TCACCTCTTAAACTAGTTTT+TGG + chr4_4:50702557-50702576 Msa0686270:three_prime_UTR 30.0%
! ACTCCAAAAACTAGTTTAAG+AGG - chr4_4:50702563-50702582 None:intergenic 30.0%
!!! TAAACTAGTTTTTGGAGTTG+AGG + chr4_4:50702565-50702584 Msa0686270:three_prime_UTR 30.0%
!!! TCTACCCTAGTTTTGTTTTT+GGG + chr4_4:50702621-50702640 Msa0686270:three_prime_UTR 30.0%
CTCAAGAACAACAATTTCAA+TGG - chr4_4:50702807-50702826 None:intergenic 30.0%
ATAAATAGCCACTCTTATTC+GGG - chr4_4:50703136-50703155 None:intergenic 30.0%
! TAATTTTGAACTACAGGCTT+TGG + chr4_4:50703387-50703406 Msa0686270:three_prime_UTR 30.0%
! AATTTTGAACTACAGGCTTT+GGG + chr4_4:50703388-50703407 Msa0686270:three_prime_UTR 30.0%
!!! TCTTTTTTCGAACAAGTACA+AGG - chr4_4:50703492-50703511 None:intergenic 30.0%
! AAAACTAGTTTAAGAGGTGA+AGG - chr4_4:50702557-50702576 None:intergenic 30.0%
!!! TCACCTCTTAAACTAGTTTT+TGG + chr4_4:50702557-50702576 Msa0686270:three_prime_UTR 30.0%
! ACTCCAAAAACTAGTTTAAG+AGG - chr4_4:50702563-50702582 None:intergenic 30.0%
!!! TAAACTAGTTTTTGGAGTTG+AGG + chr4_4:50702565-50702584 Msa0686270:three_prime_UTR 30.0%
!!! TCTACCCTAGTTTTGTTTTT+GGG + chr4_4:50702621-50702640 Msa0686270:three_prime_UTR 30.0%
CTCAAGAACAACAATTTCAA+TGG - chr4_4:50702807-50702826 None:intergenic 30.0%
ATAAATAGCCACTCTTATTC+GGG - chr4_4:50703136-50703155 None:intergenic 30.0%
! TAATTTTGAACTACAGGCTT+TGG + chr4_4:50703387-50703406 Msa0686270:three_prime_UTR 30.0%
! AATTTTGAACTACAGGCTTT+GGG + chr4_4:50703388-50703407 Msa0686270:three_prime_UTR 30.0%
!!! TCTTTTTTCGAACAAGTACA+AGG - chr4_4:50703492-50703511 None:intergenic 30.0%
AAAGAAGGCAAAGAAAAAGC+AGG + chr4_4:50702312-50702331 Msa0686270:CDS 35.0%
AGTTAGTACCATCTTGGAAT+TGG - chr4_4:50702602-50702621 None:intergenic 35.0%
!!! CTCTACCCTAGTTTTGTTTT+TGG + chr4_4:50702620-50702639 Msa0686270:three_prime_UTR 35.0%
TTGGCCCAAAAACAAAACTA+GGG - chr4_4:50702628-50702647 None:intergenic 35.0%
! TGTGCTAAGATCTCTGTTTT+TGG + chr4_4:50702690-50702709 Msa0686270:intron 35.0%
!! GTGCTAAGATCTCTGTTTTT+GGG + chr4_4:50702691-50702710 Msa0686270:intron 35.0%
! GCAATGCATTTTGAATAAGG+TGG + chr4_4:50702778-50702797 Msa0686270:intron 35.0%
TTTCTACTAGTATACCCATC+AGG + chr4_4:50703010-50703029 Msa0686270:intron 35.0%
TTGCAAATTGATGTCTAAGC+TGG - chr4_4:50703059-50703078 None:intergenic 35.0%
CATCAATTTGCAAAAGACCA+AGG + chr4_4:50703066-50703085 Msa0686270:three_prime_UTR 35.0%
TGTACATGCAAGTTTAACCA+AGG + chr4_4:50703247-50703266 Msa0686270:three_prime_UTR 35.0%
CAACGATGCTTCGAAAAATT+TGG - chr4_4:50703288-50703307 None:intergenic 35.0%
AAAGAAGGCAAAGAAAAAGC+AGG + chr4_4:50702312-50702331 Msa0686270:CDS 35.0%
AGTTAGTACCATCTTGGAAT+TGG - chr4_4:50702602-50702621 None:intergenic 35.0%
!!! CTCTACCCTAGTTTTGTTTT+TGG + chr4_4:50702620-50702639 Msa0686270:three_prime_UTR 35.0%
TTGGCCCAAAAACAAAACTA+GGG - chr4_4:50702628-50702647 None:intergenic 35.0%
! TGTGCTAAGATCTCTGTTTT+TGG + chr4_4:50702690-50702709 Msa0686270:intron 35.0%
!! GTGCTAAGATCTCTGTTTTT+GGG + chr4_4:50702691-50702710 Msa0686270:intron 35.0%
! GCAATGCATTTTGAATAAGG+TGG + chr4_4:50702778-50702797 Msa0686270:intron 35.0%
TTTCTACTAGTATACCCATC+AGG + chr4_4:50703010-50703029 Msa0686270:intron 35.0%
TTGCAAATTGATGTCTAAGC+TGG - chr4_4:50703059-50703078 None:intergenic 35.0%
CATCAATTTGCAAAAGACCA+AGG + chr4_4:50703066-50703085 Msa0686270:three_prime_UTR 35.0%
TGTACATGCAAGTTTAACCA+AGG + chr4_4:50703247-50703266 Msa0686270:three_prime_UTR 35.0%
CAACGATGCTTCGAAAAATT+TGG - chr4_4:50703288-50703307 None:intergenic 35.0%
GGTGATCAGAAAACAAAACG+TGG + chr4_4:50702274-50702293 Msa0686270:CDS 40.0%
AAAACGTGGAGTCAATGAGA+GGG + chr4_4:50702288-50702307 Msa0686270:CDS 40.0%
AGTCAATGAGAGGGAAAAGA+AGG + chr4_4:50702297-50702316 Msa0686270:CDS 40.0%
TTCCCAGCTGATTAGAGTTA+TGG + chr4_4:50702504-50702523 Msa0686270:exon 40.0%
AGCCATAACTCTAATCAGCT+GGG - chr4_4:50702509-50702528 None:intergenic 40.0%
TAGCCATAACTCTAATCAGC+TGG - chr4_4:50702510-50702529 None:intergenic 40.0%
!!! ACTAGTTTTTGGAGTTGAGG+TGG + chr4_4:50702568-50702587 Msa0686270:three_prime_UTR 40.0%
ACCTAAGTCCAATTCCAAGA+TGG + chr4_4:50702591-50702610 Msa0686270:three_prime_UTR 40.0%
ACCATCTTGGAATTGGACTT+AGG - chr4_4:50702595-50702614 None:intergenic 40.0%
CTTGGCCCAAAAACAAAACT+AGG - chr4_4:50702629-50702648 None:intergenic 40.0%
GAAGCACAGACAAATATGCT+TGG - chr4_4:50702647-50702666 None:intergenic 40.0%
TCTTAGCACAACTTACACTC+AGG - chr4_4:50702680-50702699 None:intergenic 40.0%
GATCGTGCTCAATTATGACA+CGG + chr4_4:50702873-50702892 Msa0686270:intron 40.0%
TGTCGTGCTATATATTGGCA+CGG + chr4_4:50702951-50702970 Msa0686270:intron 40.0%
TACTAGTATACCCATCAGGA+AGG + chr4_4:50703014-50703033 Msa0686270:intron 40.0%
TCTTCAATCCCGAATAAGAG+TGG + chr4_4:50703125-50703144 Msa0686270:three_prime_UTR 40.0%
GGTGATCAGAAAACAAAACG+TGG + chr4_4:50702274-50702293 Msa0686270:CDS 40.0%
AAAACGTGGAGTCAATGAGA+GGG + chr4_4:50702288-50702307 Msa0686270:CDS 40.0%
AGTCAATGAGAGGGAAAAGA+AGG + chr4_4:50702297-50702316 Msa0686270:CDS 40.0%
TTCCCAGCTGATTAGAGTTA+TGG + chr4_4:50702504-50702523 Msa0686270:exon 40.0%
AGCCATAACTCTAATCAGCT+GGG - chr4_4:50702509-50702528 None:intergenic 40.0%
TAGCCATAACTCTAATCAGC+TGG - chr4_4:50702510-50702529 None:intergenic 40.0%
!!! ACTAGTTTTTGGAGTTGAGG+TGG + chr4_4:50702568-50702587 Msa0686270:three_prime_UTR 40.0%
ACCTAAGTCCAATTCCAAGA+TGG + chr4_4:50702591-50702610 Msa0686270:three_prime_UTR 40.0%
ACCATCTTGGAATTGGACTT+AGG - chr4_4:50702595-50702614 None:intergenic 40.0%
CTTGGCCCAAAAACAAAACT+AGG - chr4_4:50702629-50702648 None:intergenic 40.0%
GAAGCACAGACAAATATGCT+TGG - chr4_4:50702647-50702666 None:intergenic 40.0%
TCTTAGCACAACTTACACTC+AGG - chr4_4:50702680-50702699 None:intergenic 40.0%
GATCGTGCTCAATTATGACA+CGG + chr4_4:50702873-50702892 Msa0686270:intron 40.0%
TGTCGTGCTATATATTGGCA+CGG + chr4_4:50702951-50702970 Msa0686270:intron 40.0%
TACTAGTATACCCATCAGGA+AGG + chr4_4:50703014-50703033 Msa0686270:intron 40.0%
TCTTCAATCCCGAATAAGAG+TGG + chr4_4:50703125-50703144 Msa0686270:three_prime_UTR 40.0%
AGAACTCATCCGTGAAGAAG+AGG + chr4_4:50702234-50702253 Msa0686270:CDS 45.0%
CAAAACGTGGAGTCAATGAG+AGG + chr4_4:50702287-50702306 Msa0686270:CDS 45.0%
GGGTAGAGTTAGTACCATCT+TGG - chr4_4:50702608-50702627 None:intergenic 45.0%
CGCACTGTCGTGCTATATAT+TGG + chr4_4:50702946-50702965 Msa0686270:intron 45.0%
CGATCATAGACCTTCCTGAT+GGG - chr4_4:50703027-50703046 None:intergenic 45.0%
TCGATCATAGACCTTCCTGA+TGG - chr4_4:50703028-50703047 None:intergenic 45.0%
TCAAGTGATCGACAAGACCA+GGG + chr4_4:50703092-50703111 Msa0686270:three_prime_UTR 45.0%
TTGAAGATCCTTCACAACCC+TGG - chr4_4:50703112-50703131 None:intergenic 45.0%
CTTTGGGCTGCTATATGCTT+TGG + chr4_4:50703404-50703423 Msa0686270:three_prime_UTR 45.0%
CGAACAAGTACAAGGAGTCA+AGG - chr4_4:50703484-50703503 None:intergenic 45.0%
AGAACTCATCCGTGAAGAAG+AGG + chr4_4:50702234-50702253 Msa0686270:CDS 45.0%
CAAAACGTGGAGTCAATGAG+AGG + chr4_4:50702287-50702306 Msa0686270:CDS 45.0%
GGGTAGAGTTAGTACCATCT+TGG - chr4_4:50702608-50702627 None:intergenic 45.0%
CGCACTGTCGTGCTATATAT+TGG + chr4_4:50702946-50702965 Msa0686270:intron 45.0%
CGATCATAGACCTTCCTGAT+GGG - chr4_4:50703027-50703046 None:intergenic 45.0%
TCGATCATAGACCTTCCTGA+TGG - chr4_4:50703028-50703047 None:intergenic 45.0%
TCAAGTGATCGACAAGACCA+GGG + chr4_4:50703092-50703111 Msa0686270:three_prime_UTR 45.0%
TTGAAGATCCTTCACAACCC+TGG - chr4_4:50703112-50703131 None:intergenic 45.0%
CTTTGGGCTGCTATATGCTT+TGG + chr4_4:50703404-50703423 Msa0686270:three_prime_UTR 45.0%
CGAACAAGTACAAGGAGTCA+AGG - chr4_4:50703484-50703503 None:intergenic 45.0%
!! TCCGTGAAGAAGAGGCATCA+TGG + chr4_4:50702242-50702261 Msa0686270:CDS 50.0%
GCCATGATGCCTCTTCTTCA+CGG - chr4_4:50702246-50702265 None:intergenic 50.0%
GCACAACTTACACTCAGGAC+TGG - chr4_4:50702675-50702694 None:intergenic 50.0%
ACGGTCGTGTTTGTTTGCAC+TGG + chr4_4:50702892-50702911 Msa0686270:intron 50.0%
TGTCGATCACTTGAGTGCCT+TGG - chr4_4:50703086-50703105 None:intergenic 50.0%
CTCAAGTGATCGACAAGACC+AGG + chr4_4:50703091-50703110 Msa0686270:three_prime_UTR 50.0%
GGTCAGTCGATCAAGAACCT+TGG - chr4_4:50703267-50703286 None:intergenic 50.0%
!! TCCGTGAAGAAGAGGCATCA+TGG + chr4_4:50702242-50702261 Msa0686270:CDS 50.0%
GCCATGATGCCTCTTCTTCA+CGG - chr4_4:50702246-50702265 None:intergenic 50.0%
GCACAACTTACACTCAGGAC+TGG - chr4_4:50702675-50702694 None:intergenic 50.0%
ACGGTCGTGTTTGTTTGCAC+TGG + chr4_4:50702892-50702911 Msa0686270:intron 50.0%
TGTCGATCACTTGAGTGCCT+TGG - chr4_4:50703086-50703105 None:intergenic 50.0%
CTCAAGTGATCGACAAGACC+AGG + chr4_4:50703091-50703110 Msa0686270:three_prime_UTR 50.0%
GGTCAGTCGATCAAGAACCT+TGG - chr4_4:50703267-50703286 None:intergenic 50.0%
! GAGGCATCATGGCAAGCTGA+AGG + chr4_4:50702253-50702272 Msa0686270:CDS 55.0%
CGACAAGACCAGGGTTGTGA+AGG + chr4_4:50703101-50703120 Msa0686270:three_prime_UTR 55.0%
! GAGGCATCATGGCAAGCTGA+AGG + chr4_4:50702253-50702272 Msa0686270:CDS 55.0%
CGACAAGACCAGGGTTGTGA+AGG + chr4_4:50703101-50703120 Msa0686270:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50702208 50703525 50702208 ID=Msa0686270;Name=Msa0686270
chr4_4 mRNA 50702208 50703525 50702208 ID=Msa0686270-mRNA-1;Parent=Msa0686270;Name=Msa0686270-mRNA-1;_AED=0.09;_eAED=0.15;_QI=0|1|0.66|1|0.5|0.33|3|675|59
chr4_4 exon 50702208 50702333 50702208 ID=Msa0686270-mRNA-1:exon:13020;Parent=Msa0686270-mRNA-1
chr4_4 exon 50702461 50702683 50702461 ID=Msa0686270-mRNA-1:exon:13021;Parent=Msa0686270-mRNA-1
chr4_4 exon 50703020 50703525 50703020 ID=Msa0686270-mRNA-1:exon:13022;Parent=Msa0686270-mRNA-1
chr4_4 CDS 50702208 50702333 50702208 ID=Msa0686270-mRNA-1:cds;Parent=Msa0686270-mRNA-1
chr4_4 CDS 50702461 50702514 50702461 ID=Msa0686270-mRNA-1:cds;Parent=Msa0686270-mRNA-1
chr4_4 three_prime_UTR 50702515 50702683 50702515 ID=Msa0686270-mRNA-1:three_prime_utr;Parent=Msa0686270-mRNA-1
chr4_4 three_prime_UTR 50703020 50703525 50703020 ID=Msa0686270-mRNA-1:three_prime_utr;Parent=Msa0686270-mRNA-1
Gene Sequence

>Msa0686270

GAAGCTGTTGCTCTAAAGAGACAAGAAGAACTCATCCGTGAAGAAGAGGCATCATGGCAAGCTGAAGGTGATCAGAAAACAAAACGTGGAGTCAATGAGAGGGAAAAGAAGGCAAAGAAAAAGCAGACTGAAATGCAATTGTCTGACAATTATATGAGATCTGTCGAGATTCCCAGCTGA
Protein sequence

>Msa0686270

EAVALKRQEELIREEEASWQAEGDQKTKRGVNEREKKAKKKQTEMQLSDNYMRSVEIPS*