Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0598790 | GAU47905.1 | 90.476 | 42 | 4 | 0 | 1 | 42 | 407 | 448 | 1.75e-14 | 77.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0598790 | A0A2Z6PEI7 | 90.476 | 42 | 4 | 0 | 1 | 42 | 407 | 448 | 8.38e-15 | 77.0 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0009420 | Msa0598790 | 0.808568 | 2.905304e-50 | -8.615850e-47 |
| Msa0015390 | Msa0598790 | 0.809662 | 1.693546e-50 | -8.615850e-47 |
| Msa0064970 | Msa0598790 | 0.805525 | 1.279837e-49 | -8.615850e-47 |
| Msa0117710 | Msa0598790 | 0.818393 | 2.006305e-52 | -8.615850e-47 |
| Msa0201070 | Msa0598790 | 0.817992 | 2.472507e-52 | -8.615850e-47 |
| Msa0220740 | Msa0598790 | 0.821894 | 3.167094e-53 | -8.615850e-47 |
| Msa0222890 | Msa0598790 | 0.805334 | 1.403727e-49 | -8.615850e-47 |
| Msa0237750 | Msa0598790 | 0.881065 | 3.270871e-70 | -8.615850e-47 |
| Msa0402290 | Msa0598790 | 0.890067 | 1.376959e-73 | -8.615850e-47 |
| Msa0411390 | Msa0598790 | 0.803445 | 3.474210e-49 | -8.615850e-47 |
| Msa0434000 | Msa0598790 | 0.809089 | 2.247757e-50 | -8.615850e-47 |
| Msa0342750 | Msa0598790 | 0.810392 | 1.179235e-50 | -8.615850e-47 |
| Msa0355220 | Msa0598790 | 0.896874 | 2.424440e-76 | -8.615850e-47 |
| Msa0362450 | Msa0598790 | 0.816366 | 5.739335e-52 | -8.615850e-47 |
| Msa0493000 | Msa0598790 | 0.842260 | 2.883324e-58 | -8.615850e-47 |
| Msa0537720 | Msa0598790 | 0.803991 | 2.676183e-49 | -8.615850e-47 |
| Msa0554740 | Msa0598790 | 0.803293 | 3.734793e-49 | -8.615850e-47 |
| Msa0585230 | Msa0598790 | 0.807210 | 5.649175e-50 | -8.615850e-47 |
| Msa0598780 | Msa0598790 | 0.857340 | 1.744580e-62 | -8.615850e-47 |
| Msa0598790 | Msa0626820 | 0.804004 | 2.659974e-49 | -8.615850e-47 |
| Msa0598790 | Msa0649110 | 0.813737 | 2.199355e-51 | -8.615850e-47 |
| Msa0598790 | Msa0686270 | 0.878194 | 3.421054e-69 | -8.615850e-47 |
| Msa0598790 | Msa0745790 | 0.800778 | 1.228396e-48 | -8.615850e-47 |
| Msa0598790 | Msa0748970 | 0.802329 | 5.908578e-49 | -8.615850e-47 |
| Msa0598790 | Msa0766630 | 0.825711 | 4.038909e-54 | -8.615850e-47 |
| Msa0598790 | Msa0802830 | 0.800203 | 1.609374e-48 | -8.615850e-47 |
| Msa0598790 | Msa0824390 | 0.805083 | 1.584009e-49 | -8.615850e-47 |
| Msa0598790 | Msa0832340 | 0.800328 | 1.517169e-48 | -8.615850e-47 |
| Msa0598790 | Msa0900980 | 0.801953 | 7.060673e-49 | -8.615850e-47 |
| Msa0598790 | Msa0907260 | 0.824164 | 9.361577e-54 | -8.615850e-47 |
| Msa0598790 | Msa0913060 | 0.812782 | 3.564328e-51 | -8.615850e-47 |
| Msa0598790 | Msa0925290 | 0.811948 | 5.422982e-51 | -8.615850e-47 |
| Msa0598790 | Msa0956270 | 0.815855 | 7.466200e-52 | -8.615850e-47 |
| Msa0598790 | Msa0967280 | 0.839855 | 1.234062e-57 | -8.615850e-47 |
| Msa0598790 | Msa0976670 | 0.834342 | 3.168049e-56 | -8.615850e-47 |
| Msa0598790 | Msa0998680 | 0.939611 | 9.507517e-100 | -8.615850e-47 |
| Msa0598790 | Msa0998690 | 0.841176 | 5.569188e-58 | -8.615850e-47 |
| Msa0598790 | Msa1014540 | 0.803314 | 3.698137e-49 | -8.615850e-47 |
| Msa0598790 | Msa1069990 | 0.800278 | 1.553218e-48 | -8.615850e-47 |
| Msa0598790 | Msa1073240 | 0.923756 | 1.744738e-89 | -8.615850e-47 |
| Msa0598790 | Msa1075650 | 0.804477 | 2.120180e-49 | -8.615850e-47 |
| Msa0598790 | Msa1121490 | 0.809353 | 1.973132e-50 | -8.615850e-47 |
| Msa0598790 | Msa1241130 | -0.831081 | 2.042935e-55 | -8.615850e-47 |
| Msa0598790 | Msa1397860 | 0.902339 | 1.072116e-78 | -8.615850e-47 |
| Msa0598790 | Msa1420290 | 0.807614 | 4.638491e-50 | -8.615850e-47 |
| Msa0598790 | Msa1424150 | 0.927088 | 1.911961e-91 | -8.615850e-47 |
| Msa0598790 | Msa1448640 | 0.800161 | 1.640881e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0598790 | MtrunA17_Chr1g0186411 | 97.143 | 35 | 1 | 0 | 1 | 35 | 402 | 436 | 4.90e-16 | 69.7 |
| Msa0598790 | MtrunA17_Chr1g0168221 | 80.000 | 35 | 7 | 0 | 1 | 35 | 406 | 440 | 4.05e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGCTAAATTATTCGTATA+AGG | 0.295433 | 4_2:-36530565 | Msa0598790:three_prime_UTR |
| AGTTAGTACCATCTTGGAAT+TGG | 0.364864 | 4_2:+36530489 | Msa0598780:intron |
| GCCATGATGCCTCTTCTTCA+CGG | 0.372818 | 4_2:+36530845 | None:intergenic |
| TTCCCAGCTGATTAGAGTTA+TGG | 0.402928 | 4_2:-36530584 | Msa0598790:exon |
| TAGCCATAACTCTAATCAGC+TGG | 0.446311 | 4_2:+36530581 | None:intergenic |
| ACCATCTTGGAATTGGACTT+AGG | 0.515604 | 4_2:+36530496 | Msa0598780:intron |
| GGGTAGAGTTAGTACCATCT+TGG | 0.533104 | 4_2:+36530483 | Msa0598780:intron |
| CAAAACGTGGAGTCAATGAG+AGG | 0.543462 | 4_2:-36530801 | Msa0598790:CDS |
| AAAACTAGTTTAAGAGGTGA+AGG | 0.552582 | 4_2:+36530534 | None:intergenic |
| AGAACTCATCCGTGAAGAAG+AGG | 0.564259 | 4_2:-36530854 | Msa0598790:CDS |
| AGTCAATGAGAGGGAAAAGA+AGG | 0.577856 | 4_2:-36530791 | Msa0598790:CDS |
| TCCGTGAAGAAGAGGCATCA+TGG | 0.580342 | 4_2:-36530846 | Msa0598790:CDS |
| GAGGCATCATGGCAAGCTGA+AGG | 0.593174 | 4_2:-36530835 | Msa0598790:CDS |
| AGCCATAACTCTAATCAGCT+GGG | 0.594139 | 4_2:+36530582 | None:intergenic |
| ACCTAAGTCCAATTCCAAGA+TGG | 0.606889 | 4_2:-36530497 | Msa0598790:three_prime_UTR |
| AAAACGTGGAGTCAATGAGA+GGG | 0.644020 | 4_2:-36530800 | Msa0598790:CDS |
| GGTGATCAGAAAACAAAACG+TGG | 0.704649 | 4_2:-36530814 | Msa0598790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTGAAATTGCATCATTA+AGG | - | chr4_2:36530598-36530617 | Msa0598790:CDS | 20.0% |
| ! | ATGGCTAAATTATTCGTATA+AGG | - | chr4_2:36530777-36530796 | Msa0598790:CDS | 25.0% |
| ! | AAAACTAGTTTAAGAGGTGA+AGG | + | chr4_2:36530811-36530830 | None:intergenic | 30.0% |
| !!! | TCACCTCTTAAACTAGTTTT+TGG | - | chr4_2:36530811-36530830 | Msa0598790:CDS | 30.0% |
| ! | ACTCCAAAAACTAGTTTAAG+AGG | + | chr4_2:36530817-36530836 | None:intergenic | 30.0% |
| !!! | TAAACTAGTTTTTGGAGTTG+AGG | - | chr4_2:36530819-36530838 | Msa0598790:CDS | 30.0% |
| !!! | TCTACCCTAGTTTTGTTTTT+GGG | - | chr4_2:36530875-36530894 | Msa0598790:CDS | 30.0% |
| AAAGAAGGCAAAGAAAAAGC+AGG | - | chr4_2:36530566-36530585 | Msa0598790:three_prime_UTR | 35.0% | |
| AGTTAGTACCATCTTGGAAT+TGG | + | chr4_2:36530856-36530875 | None:intergenic | 35.0% | |
| !!! | CTCTACCCTAGTTTTGTTTT+TGG | - | chr4_2:36530874-36530893 | Msa0598790:CDS | 35.0% |
| TTGGCCCAAAAACAAAACTA+GGG | + | chr4_2:36530882-36530901 | None:intergenic | 35.0% | |
| GGTGATCAGAAAACAAAACG+TGG | - | chr4_2:36530528-36530547 | Msa0598790:three_prime_UTR | 40.0% | |
| AAAACGTGGAGTCAATGAGA+GGG | - | chr4_2:36530542-36530561 | Msa0598790:three_prime_UTR | 40.0% | |
| AGTCAATGAGAGGGAAAAGA+AGG | - | chr4_2:36530551-36530570 | Msa0598790:three_prime_UTR | 40.0% | |
| TTCCCAGCTGATTAGAGTTA+TGG | - | chr4_2:36530758-36530777 | Msa0598790:intron | 40.0% | |
| AGCCATAACTCTAATCAGCT+GGG | + | chr4_2:36530763-36530782 | None:intergenic | 40.0% | |
| TAGCCATAACTCTAATCAGC+TGG | + | chr4_2:36530764-36530783 | None:intergenic | 40.0% | |
| ACCTAAGTCCAATTCCAAGA+TGG | - | chr4_2:36530845-36530864 | Msa0598790:CDS | 40.0% | |
| ACCATCTTGGAATTGGACTT+AGG | + | chr4_2:36530849-36530868 | None:intergenic | 40.0% | |
| CTTGGCCCAAAAACAAAACT+AGG | + | chr4_2:36530883-36530902 | None:intergenic | 40.0% | |
| AGAACTCATCCGTGAAGAAG+AGG | - | chr4_2:36530488-36530507 | Msa0598790:three_prime_UTR | 45.0% | |
| CAAAACGTGGAGTCAATGAG+AGG | - | chr4_2:36530541-36530560 | Msa0598790:three_prime_UTR | 45.0% | |
| GGGTAGAGTTAGTACCATCT+TGG | + | chr4_2:36530862-36530881 | None:intergenic | 45.0% | |
| !! | TCCGTGAAGAAGAGGCATCA+TGG | - | chr4_2:36530496-36530515 | Msa0598790:three_prime_UTR | 50.0% |
| GCCATGATGCCTCTTCTTCA+CGG | + | chr4_2:36530500-36530519 | Msa0598780:intron | 50.0% | |
| ! | GAGGCATCATGGCAAGCTGA+AGG | - | chr4_2:36530507-36530526 | Msa0598790:three_prime_UTR | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 36530462 | 36530902 | 36530462 | ID=Msa0598790;Name=Msa0598790 |
| chr4_2 | mRNA | 36530462 | 36530902 | 36530462 | ID=Msa0598790-mRNA-1;Parent=Msa0598790;Name=Msa0598790-mRNA-1;_AED=0.27;_eAED=0.42;_QI=0|1|0.5|1|1|1|2|134|59 |
| chr4_2 | exon | 36530462 | 36530649 | 36530462 | ID=Msa0598790-mRNA-1:exon:8288;Parent=Msa0598790-mRNA-1 |
| chr4_2 | exon | 36530777 | 36530902 | 36530777 | ID=Msa0598790-mRNA-1:exon:8287;Parent=Msa0598790-mRNA-1 |
| chr4_2 | CDS | 36530777 | 36530902 | 36530777 | ID=Msa0598790-mRNA-1:cds;Parent=Msa0598790-mRNA-1 |
| chr4_2 | CDS | 36530596 | 36530649 | 36530596 | ID=Msa0598790-mRNA-1:cds;Parent=Msa0598790-mRNA-1 |
| chr4_2 | three_prime_UTR | 36530462 | 36530595 | 36530462 | ID=Msa0598790-mRNA-1:three_prime_utr;Parent=Msa0598790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
