Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1073240 | A0A2Z6PEI7 | 90.476 | 42 | 4 | 0 | 19 | 60 | 407 | 448 | 7.98e-15 | 77.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0686270 | Msa1073240 | 0.830552 | 2.754047e-55 | -8.615850e-47 |
| Msa0201070 | Msa1073240 | 0.813756 | 2.178271e-51 | -8.615850e-47 |
| Msa0237750 | Msa1073240 | 0.817926 | 2.559850e-52 | -8.615850e-47 |
| Msa0402290 | Msa1073240 | 0.847148 | 1.389346e-59 | -8.615850e-47 |
| Msa0998680 | Msa1073240 | 0.912475 | 1.866432e-83 | -8.615850e-47 |
| Msa0998690 | Msa1073240 | 0.801195 | 1.009760e-48 | -8.615850e-47 |
| Msa1073240 | Msa1397860 | 0.836859 | 7.311242e-57 | -8.615850e-47 |
| Msa1073240 | Msa1424150 | 0.991834 | 8.486069e-190 | -8.615850e-47 |
| Msa0355220 | Msa1073240 | 0.838302 | 3.118227e-57 | -8.615850e-47 |
| Msa0493000 | Msa1073240 | 0.814447 | 1.533947e-51 | -8.615850e-47 |
| Msa0598780 | Msa1073240 | 0.806431 | 8.253087e-50 | -8.615850e-47 |
| Msa0598790 | Msa1073240 | 0.923756 | 1.744738e-89 | -8.615850e-47 |
| Msa0967280 | Msa1073240 | 0.814969 | 1.175211e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1073240 | MtrunA17_Chr1g0186411 | 97.143 | 35 | 1 | 0 | 19 | 53 | 402 | 436 | 4.51e-16 | 70.9 |
| Msa1073240 | MtrunA17_Chr1g0168221 | 80.000 | 35 | 7 | 0 | 19 | 53 | 406 | 440 | 3.03e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTCACGTGAGATTCAAAA+TGG | 0.279377 | 7_2:+70691100 | None:intergenic |
| ATGGCTAAATTATTCGTATA+AGG | 0.295433 | 7_2:-70690414 | Msa1073240:three_prime_UTR |
| AGTTAGTACCATCTTGGAAT+TGG | 0.364864 | 7_2:+70690338 | None:intergenic |
| GCCATGATGCCTCTTCTTCA+CGG | 0.372818 | 7_2:+70690694 | None:intergenic |
| TTCCCAGCTGATTAGAGTTA+TGG | 0.402928 | 7_2:-70690433 | Msa1073240:exon |
| TAGCCATAACTCTAATCAGC+TGG | 0.446311 | 7_2:+70690430 | None:intergenic |
| ACTCACGTGAGATTCAAAAT+GGG | 0.510554 | 7_2:+70691101 | None:intergenic |
| ACCATCTTGGAATTGGACTT+AGG | 0.515604 | 7_2:+70690345 | None:intergenic |
| GGGTAGAGTTAGTACCATCT+TGG | 0.533104 | 7_2:+70690332 | None:intergenic |
| CAAAACGTGGAGTCAATGAG+AGG | 0.543462 | 7_2:-70690650 | Msa1073240:CDS |
| AAAACTAGTTTAAGAGGTGA+AGG | 0.552582 | 7_2:+70690383 | None:intergenic |
| AGAACTCATCCGTGAAGAAG+AGG | 0.564259 | 7_2:-70690703 | Msa1073240:CDS |
| AGTCAATGAGAGGGAAAAGA+AGG | 0.577856 | 7_2:-70690640 | Msa1073240:CDS |
| TCCGTGAAGAAGAGGCATCA+TGG | 0.580342 | 7_2:-70690695 | Msa1073240:CDS |
| GAGGCATCATGGCAAGCTGA+AGG | 0.593174 | 7_2:-70690684 | Msa1073240:CDS |
| AGCCATAACTCTAATCAGCT+GGG | 0.594139 | 7_2:+70690431 | None:intergenic |
| ACCTAAGTCCAATTCCAAGA+TGG | 0.606889 | 7_2:-70690346 | Msa1073240:three_prime_UTR |
| CTCACGTGAGATTCAAAATG+GGG | 0.622186 | 7_2:+70691102 | None:intergenic |
| AAAACGTGGAGTCAATGAGA+GGG | 0.644020 | 7_2:-70690649 | Msa1073240:CDS |
| GGTGATCAGAAAACAAAACG+TGG | 0.704649 | 7_2:-70690663 | Msa1073240:CDS |
| TCACGTGAGATTCAAAATGG+GGG | 0.709510 | 7_2:+70691103 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATTTTTTATTATTTTTT+TGG | - | chr7_2:70690645-70690664 | Msa1073240:CDS | 0.0% |
| !!! | TTAATTTTTTATTATTTTTT+TGG | - | chr7_2:70690645-70690664 | Msa1073240:CDS | 0.0% |
| !!! | AAGATGAAATTTAAGTTAAT+GGG | + | chr7_2:70690473-70690492 | None:intergenic | 15.0% |
| !!! | AAGATGAAATTTAAGTTAAT+GGG | + | chr7_2:70690473-70690492 | None:intergenic | 15.0% |
| !!! | TTCTGAAAATATGAAGAAAA+TGG | + | chr7_2:70690428-70690447 | None:intergenic | 20.0% |
| !!! | GAAGATGAAATTTAAGTTAA+TGG | + | chr7_2:70690474-70690493 | None:intergenic | 20.0% |
| !!! | TATTTTCAGCAAAAAAATTG+AGG | - | chr7_2:70690686-70690705 | Msa1073240:CDS | 20.0% |
| !!! | TTCTGAAAATATGAAGAAAA+TGG | + | chr7_2:70690428-70690447 | None:intergenic | 20.0% |
| !!! | GAAGATGAAATTTAAGTTAA+TGG | + | chr7_2:70690474-70690493 | None:intergenic | 20.0% |
| !!! | TATTTTCAGCAAAAAAATTG+AGG | - | chr7_2:70690686-70690705 | Msa1073240:CDS | 20.0% |
| ! | TGAAAATATGAAGAAAATGG+TGG | + | chr7_2:70690425-70690444 | None:intergenic | 25.0% |
| !! | TGAAATTTAAGTTAATGGGT+AGG | + | chr7_2:70690469-70690488 | None:intergenic | 25.0% |
| ! | TTTCACTTTCCATTAAAGTT+TGG | - | chr7_2:70690517-70690536 | Msa1073240:intron | 25.0% |
| !!! | TCTGAAAACTTTTAACTTGA+AGG | - | chr7_2:70690596-70690615 | Msa1073240:intron | 25.0% |
| ! | AGATTGAAATTGCATCATTA+AGG | - | chr7_2:70690856-70690875 | Msa1073240:intron | 25.0% |
| ! | ATGGCTAAATTATTCGTATA+AGG | - | chr7_2:70691035-70691054 | Msa1073240:intron | 25.0% |
| ! | TGAAAATATGAAGAAAATGG+TGG | + | chr7_2:70690425-70690444 | None:intergenic | 25.0% |
| !! | TGAAATTTAAGTTAATGGGT+AGG | + | chr7_2:70690469-70690488 | None:intergenic | 25.0% |
| ! | TTTCACTTTCCATTAAAGTT+TGG | - | chr7_2:70690517-70690536 | Msa1073240:intron | 25.0% |
| !!! | TCTGAAAACTTTTAACTTGA+AGG | - | chr7_2:70690596-70690615 | Msa1073240:intron | 25.0% |
| ! | AGATTGAAATTGCATCATTA+AGG | - | chr7_2:70690856-70690875 | Msa1073240:intron | 25.0% |
| ! | ATGGCTAAATTATTCGTATA+AGG | - | chr7_2:70691035-70691054 | Msa1073240:intron | 25.0% |
| TTGCAATCTCCAAACTTTAA+TGG | + | chr7_2:70690529-70690548 | None:intergenic | 30.0% | |
| AAAAATTGAGGTTGCTTAAC+AGG | - | chr7_2:70690698-70690717 | Msa1073240:CDS | 30.0% | |
| ! | AAAACTAGTTTAAGAGGTGA+AGG | + | chr7_2:70691069-70691088 | None:intergenic | 30.0% |
| !!! | TCACCTCTTAAACTAGTTTT+TGG | - | chr7_2:70691069-70691088 | Msa1073240:intron | 30.0% |
| ! | ACTCCAAAAACTAGTTTAAG+AGG | + | chr7_2:70691075-70691094 | None:intergenic | 30.0% |
| !!! | TAAACTAGTTTTTGGAGTTG+AGG | - | chr7_2:70691077-70691096 | Msa1073240:intron | 30.0% |
| !!! | TCTACCCTAGTTTTGTTTTT+GGG | - | chr7_2:70691133-70691152 | Msa1073240:CDS | 30.0% |
| TTGCAATCTCCAAACTTTAA+TGG | + | chr7_2:70690529-70690548 | None:intergenic | 30.0% | |
| AAAAATTGAGGTTGCTTAAC+AGG | - | chr7_2:70690698-70690717 | Msa1073240:CDS | 30.0% | |
| ! | AAAACTAGTTTAAGAGGTGA+AGG | + | chr7_2:70691069-70691088 | None:intergenic | 30.0% |
| !!! | TCACCTCTTAAACTAGTTTT+TGG | - | chr7_2:70691069-70691088 | Msa1073240:intron | 30.0% |
| ! | ACTCCAAAAACTAGTTTAAG+AGG | + | chr7_2:70691075-70691094 | None:intergenic | 30.0% |
| !!! | TAAACTAGTTTTTGGAGTTG+AGG | - | chr7_2:70691077-70691096 | Msa1073240:intron | 30.0% |
| !!! | TCTACCCTAGTTTTGTTTTT+GGG | - | chr7_2:70691133-70691152 | Msa1073240:CDS | 30.0% |
| ACTCACGTGAGATTCAAAAT+GGG | + | chr7_2:70690351-70690370 | None:intergenic | 35.0% | |
| TCTTTGTGAGATTCAAGTGA+AGG | + | chr7_2:70690388-70690407 | None:intergenic | 35.0% | |
| TTGGAGATTGCAACTTAGTT+TGG | - | chr7_2:70690536-70690555 | Msa1073240:intron | 35.0% | |
| AAAGAAGGCAAAGAAAAAGC+AGG | - | chr7_2:70690824-70690843 | Msa1073240:intron | 35.0% | |
| AGTTAGTACCATCTTGGAAT+TGG | + | chr7_2:70691114-70691133 | None:intergenic | 35.0% | |
| !!! | CTCTACCCTAGTTTTGTTTT+TGG | - | chr7_2:70691132-70691151 | Msa1073240:CDS | 35.0% |
| TTGGCCCAAAAACAAAACTA+GGG | + | chr7_2:70691140-70691159 | None:intergenic | 35.0% | |
| ACTCACGTGAGATTCAAAAT+GGG | + | chr7_2:70690351-70690370 | None:intergenic | 35.0% | |
| TCTTTGTGAGATTCAAGTGA+AGG | + | chr7_2:70690388-70690407 | None:intergenic | 35.0% | |
| TTGGAGATTGCAACTTAGTT+TGG | - | chr7_2:70690536-70690555 | Msa1073240:intron | 35.0% | |
| AAAGAAGGCAAAGAAAAAGC+AGG | - | chr7_2:70690824-70690843 | Msa1073240:intron | 35.0% | |
| AGTTAGTACCATCTTGGAAT+TGG | + | chr7_2:70691114-70691133 | None:intergenic | 35.0% | |
| !!! | CTCTACCCTAGTTTTGTTTT+TGG | - | chr7_2:70691132-70691151 | Msa1073240:CDS | 35.0% |
| TTGGCCCAAAAACAAAACTA+GGG | + | chr7_2:70691140-70691159 | None:intergenic | 35.0% | |
| AGATTCAAAATGGGGGTTGA+TGG | + | chr7_2:70690342-70690361 | None:intergenic | 40.0% | |
| TCACGTGAGATTCAAAATGG+GGG | + | chr7_2:70690349-70690368 | None:intergenic | 40.0% | |
| CTCACGTGAGATTCAAAATG+GGG | + | chr7_2:70690350-70690369 | None:intergenic | 40.0% | |
| GACTCACGTGAGATTCAAAA+TGG | + | chr7_2:70690352-70690371 | None:intergenic | 40.0% | |
| GGTGATCAGAAAACAAAACG+TGG | - | chr7_2:70690786-70690805 | Msa1073240:intron | 40.0% | |
| AAAACGTGGAGTCAATGAGA+GGG | - | chr7_2:70690800-70690819 | Msa1073240:intron | 40.0% | |
| AGTCAATGAGAGGGAAAAGA+AGG | - | chr7_2:70690809-70690828 | Msa1073240:intron | 40.0% | |
| TTCCCAGCTGATTAGAGTTA+TGG | - | chr7_2:70691016-70691035 | Msa1073240:intron | 40.0% | |
| AGCCATAACTCTAATCAGCT+GGG | + | chr7_2:70691021-70691040 | None:intergenic | 40.0% | |
| TAGCCATAACTCTAATCAGC+TGG | + | chr7_2:70691022-70691041 | None:intergenic | 40.0% | |
| ACCTAAGTCCAATTCCAAGA+TGG | - | chr7_2:70691103-70691122 | Msa1073240:intron | 40.0% | |
| ACCATCTTGGAATTGGACTT+AGG | + | chr7_2:70691107-70691126 | None:intergenic | 40.0% | |
| CTTGGCCCAAAAACAAAACT+AGG | + | chr7_2:70691141-70691160 | None:intergenic | 40.0% | |
| AGATTCAAAATGGGGGTTGA+TGG | + | chr7_2:70690342-70690361 | None:intergenic | 40.0% | |
| TCACGTGAGATTCAAAATGG+GGG | + | chr7_2:70690349-70690368 | None:intergenic | 40.0% | |
| CTCACGTGAGATTCAAAATG+GGG | + | chr7_2:70690350-70690369 | None:intergenic | 40.0% | |
| GACTCACGTGAGATTCAAAA+TGG | + | chr7_2:70690352-70690371 | None:intergenic | 40.0% | |
| GGTGATCAGAAAACAAAACG+TGG | - | chr7_2:70690786-70690805 | Msa1073240:intron | 40.0% | |
| AAAACGTGGAGTCAATGAGA+GGG | - | chr7_2:70690800-70690819 | Msa1073240:intron | 40.0% | |
| AGTCAATGAGAGGGAAAAGA+AGG | - | chr7_2:70690809-70690828 | Msa1073240:intron | 40.0% | |
| TTCCCAGCTGATTAGAGTTA+TGG | - | chr7_2:70691016-70691035 | Msa1073240:intron | 40.0% | |
| AGCCATAACTCTAATCAGCT+GGG | + | chr7_2:70691021-70691040 | None:intergenic | 40.0% | |
| TAGCCATAACTCTAATCAGC+TGG | + | chr7_2:70691022-70691041 | None:intergenic | 40.0% | |
| ACCTAAGTCCAATTCCAAGA+TGG | - | chr7_2:70691103-70691122 | Msa1073240:intron | 40.0% | |
| ACCATCTTGGAATTGGACTT+AGG | + | chr7_2:70691107-70691126 | None:intergenic | 40.0% | |
| CTTGGCCCAAAAACAAAACT+AGG | + | chr7_2:70691141-70691160 | None:intergenic | 40.0% | |
| AGAACTCATCCGTGAAGAAG+AGG | - | chr7_2:70690746-70690765 | Msa1073240:intron | 45.0% | |
| CAAAACGTGGAGTCAATGAG+AGG | - | chr7_2:70690799-70690818 | Msa1073240:intron | 45.0% | |
| GGGTAGAGTTAGTACCATCT+TGG | + | chr7_2:70691120-70691139 | None:intergenic | 45.0% | |
| AGAACTCATCCGTGAAGAAG+AGG | - | chr7_2:70690746-70690765 | Msa1073240:intron | 45.0% | |
| CAAAACGTGGAGTCAATGAG+AGG | - | chr7_2:70690799-70690818 | Msa1073240:intron | 45.0% | |
| GGGTAGAGTTAGTACCATCT+TGG | + | chr7_2:70691120-70691139 | None:intergenic | 45.0% | |
| !! | TCCGTGAAGAAGAGGCATCA+TGG | - | chr7_2:70690754-70690773 | Msa1073240:intron | 50.0% |
| GCCATGATGCCTCTTCTTCA+CGG | + | chr7_2:70690758-70690777 | None:intergenic | 50.0% | |
| !! | TCCGTGAAGAAGAGGCATCA+TGG | - | chr7_2:70690754-70690773 | Msa1073240:intron | 50.0% |
| GCCATGATGCCTCTTCTTCA+CGG | + | chr7_2:70690758-70690777 | None:intergenic | 50.0% | |
| ! | GAGGCATCATGGCAAGCTGA+AGG | - | chr7_2:70690765-70690784 | Msa1073240:intron | 55.0% |
| ! | GAGGCATCATGGCAAGCTGA+AGG | - | chr7_2:70690765-70690784 | Msa1073240:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 70690311 | 70691160 | 70690311 | ID=Msa1073240;Name=Msa1073240 |
| chr7_2 | mRNA | 70690311 | 70691160 | 70690311 | ID=Msa1073240-mRNA-1;Parent=Msa1073240;Name=Msa1073240-mRNA-1;_AED=0.35;_eAED=0.48;_QI=0|0.5|0.33|0.66|1|1|3|134|77 |
| chr7_2 | exon | 70690311 | 70690498 | 70690311 | ID=Msa1073240-mRNA-1:exon:15995;Parent=Msa1073240-mRNA-1 |
| chr7_2 | exon | 70690626 | 70690751 | 70690626 | ID=Msa1073240-mRNA-1:exon:15994;Parent=Msa1073240-mRNA-1 |
| chr7_2 | exon | 70691107 | 70691160 | 70691107 | ID=Msa1073240-mRNA-1:exon:15993;Parent=Msa1073240-mRNA-1 |
| chr7_2 | CDS | 70691107 | 70691160 | 70691107 | ID=Msa1073240-mRNA-1:cds;Parent=Msa1073240-mRNA-1 |
| chr7_2 | CDS | 70690626 | 70690751 | 70690626 | ID=Msa1073240-mRNA-1:cds;Parent=Msa1073240-mRNA-1 |
| chr7_2 | CDS | 70690445 | 70690498 | 70690445 | ID=Msa1073240-mRNA-1:cds;Parent=Msa1073240-mRNA-1 |
| chr7_2 | three_prime_UTR | 70690311 | 70690444 | 70690311 | ID=Msa1073240-mRNA-1:three_prime_utr;Parent=Msa1073240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
