AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1430290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1430290 MtrunA17_Chr4g0046091 93.182 264 9 2 1 255 23 286 6.24e-178 490
Msa1430290 MtrunA17_Chr2g0283341 46.460 226 100 3 7 232 1 205 6.40e-66 205
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1430290 AT1G28960.3 70.635 252 62 4 14 254 42 292 7.37e-120 344
Msa1430290 AT1G28960.1 70.635 252 62 4 14 254 42 292 7.37e-120 344
Msa1430290 AT1G28960.5 70.635 252 62 4 14 254 42 292 7.37e-120 344
Msa1430290 AT1G28960.7 71.429 245 58 4 14 247 42 285 1.15e-117 338
Msa1430290 AT1G28960.6 71.429 245 58 4 14 247 42 285 1.15e-117 338
Msa1430290 AT2G33980.2 64.372 247 73 4 11 247 18 259 1.68e-103 301
Msa1430290 AT2G33980.4 64.372 247 73 4 11 247 18 259 1.68e-103 301
Msa1430290 AT2G33980.1 64.372 247 73 4 11 247 18 259 5.78e-103 301
Msa1430290 AT2G33980.3 64.372 247 73 4 11 247 18 259 1.22e-102 301
Msa1430290 AT5G45940.1 63.743 171 61 1 73 242 47 217 1.88e-74 226
Msa1430290 AT5G45940.2 67.273 110 36 0 73 182 47 156 5.79e-47 154
Msa1430290 AT5G45940.3 68.519 108 34 0 73 180 47 154 1.46e-46 153

Find 85 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCCTCTTCCTTTCTTTC+AGG 0.144264 tig0026647:-13572 None:intergenic
AACCTGTGGAACTTTGAATT+TGG 0.148694 tig0026647:-13430 None:intergenic
TGAATCATGGTAGCTGATTC+AGG 0.246118 tig0026647:-11116 None:intergenic
GTAACAACATTGACTAGTTT+TGG 0.246703 tig0026647:-11686 None:intergenic
CGAGAGGCAGAAGAGGAAAT+TGG 0.270083 tig0026647:+11656 Msa1430290:CDS
TCTTGAACCATTCTTGTCTA+AGG 0.274255 tig0026647:+11712 Msa1430290:CDS
CTCTGTTCCAAAACATCTTC+AGG 0.287071 tig0026647:-11050 None:intergenic
ATTTCTGCTCACCTTGTGTT+TGG 0.302067 tig0026647:+13616 Msa1430290:three_prime_UTR
TGGCAAAGTTGTTTCTCAAT+TGG 0.304222 tig0026647:+11088 Msa1430290:CDS
ACCTGTGGAACTTTGAATTT+GGG 0.326845 tig0026647:-13429 None:intergenic
GCAAACTGCCATCACATCTA+TGG 0.329294 tig0026647:-13809 None:intergenic
AAAGTGATGATGGGGATCTA+AGG 0.334445 tig0026647:+11191 Msa1430290:intron
GGCAAAGTTGTTTCTCAATT+GGG 0.351294 tig0026647:+11089 Msa1430290:CDS
TAGAATAAATCAAATGGAAA+AGG 0.394241 tig0026647:+13654 Msa1430290:three_prime_UTR
CGATGACAGGAACTACTCTT+AGG 0.397188 tig0026647:-13050 None:intergenic
TCCAGATCCCAAAATTAGTC+TGG 0.402451 tig0026647:-13691 None:intergenic
TGCAGCCCTAATCAAAATTC+CGG 0.409951 tig0026647:-13364 None:intergenic
TCAGGAAGAGAGAGAGTGGA+TGG 0.414184 tig0026647:+13266 Msa1430290:CDS
CCTTCCTCCGCTTTCCCACC+AGG 0.426104 tig0026647:-11605 None:intergenic
TACAATAATATATATTCGTC+AGG 0.427184 tig0026647:+13533 Msa1430290:three_prime_UTR
AGAATAAATCAAATGGAAAA+GGG 0.430085 tig0026647:+13655 Msa1430290:three_prime_UTR
TGTTTATTTGAAAGTGATGA+TGG 0.443684 tig0026647:+11181 Msa1430290:intron
GAAAGCGGAGGAAGGGGATA+AGG 0.452799 tig0026647:+11613 Msa1430290:CDS
GATCGGTAGAATAAATCAAA+TGG 0.459510 tig0026647:+13648 Msa1430290:three_prime_UTR
ACCTGAGTGAGTAGAGAGCT+TGG 0.464566 tig0026647:-11236 None:intergenic
AATACTGATTCCACTTCTGC+AGG 0.466410 tig0026647:-13114 None:intergenic
ACTTCTGCAGGATTCAGAAC+AGG 0.470641 tig0026647:-13102 None:intergenic
TGGTGGGAAAGCGGAGGAAG+GGG 0.473889 tig0026647:+11607 Msa1430290:CDS
GCTTTAAGCAGGATGAAAAT+CGG 0.478548 tig0026647:+13241 Msa1430290:intron
CCTGGTGGGAAAGCGGAGGA+AGG 0.480967 tig0026647:+11605 Msa1430290:CDS
TCCAAAACATCTTCAGGAAA+AGG 0.484289 tig0026647:-11044 None:intergenic
GGAATAATAAAATGTTGTAA+AGG 0.484974 tig0026647:+13711 Msa1430290:three_prime_UTR
ATTTATATCTCCTGAAAGAA+AGG 0.485574 tig0026647:+13562 Msa1430290:three_prime_UTR
GAAATATGAATTCCAAGTGA+TGG 0.489702 tig0026647:-13598 None:intergenic
GAGAGGCAGAAGAGGAAATT+GGG 0.494487 tig0026647:+11657 Msa1430290:CDS
TGTTCTGAATCCTGCAGAAG+TGG 0.496762 tig0026647:+13104 Msa1430290:CDS
CTGGTGGGAAAGCGGAGGAA+GGG 0.497359 tig0026647:+11606 Msa1430290:CDS
GCAGGATGAAAATCGGAGTC+AGG 0.500021 tig0026647:+13248 Msa1430290:intron
ACCTTGAGAAACATTTCTAA+AGG 0.503154 tig0026647:-13144 None:intergenic
TCTTCCAACGTTACCACTGG+TGG 0.507992 tig0026647:+10966 Msa1430290:CDS
TCCCAAATTCAAAGTTCCAC+AGG 0.509189 tig0026647:+13428 Msa1430290:CDS
CTGCATCAATTGTGTACAAA+CGG 0.513281 tig0026647:+13382 Msa1430290:CDS
CTCATATGGGGATTGACTGC+CGG 0.519065 tig0026647:+13345 Msa1430290:CDS
ATCTCCTGAAAGAAAGGAAG+AGG 0.524063 tig0026647:+13568 Msa1430290:three_prime_UTR
GGCAGAAGAGGAAATTGGGT+TGG 0.528513 tig0026647:+11661 Msa1430290:CDS
TCATGAAGTATGCCGATGAC+AGG 0.534330 tig0026647:-13063 None:intergenic
TCTTCTTCCAACGTTACCAC+TGG 0.535311 tig0026647:+10963 Msa1430290:CDS
AGAGGATGCTGTCCATCACT+TGG 0.536088 tig0026647:+13586 Msa1430290:three_prime_UTR
CAGGAAGAGAGAGAGTGGAT+GGG 0.539082 tig0026647:+13267 Msa1430290:CDS
AAGTGATGATGGGGATCTAA+GGG 0.540717 tig0026647:+11192 Msa1430290:intron
TGAAGAAAAGAAATATATTG+AGG 0.545140 tig0026647:-13507 None:intergenic
GTGTTTGGATATCTAATGAT+CGG 0.547839 tig0026647:+13631 Msa1430290:three_prime_UTR
ACCTTTAGAAATGTTTCTCA+AGG 0.548045 tig0026647:+13143 Msa1430290:CDS
GGATTCTGCTCTATGAAAGC+CGG 0.548477 tig0026647:-13408 None:intergenic
TGCAAAACGAGAGGCAGAAG+AGG 0.549211 tig0026647:+11649 Msa1430290:CDS
ATCTCAGAGACTTCTTGCGT+TGG 0.549765 tig0026647:+10989 Msa1430290:CDS
GTTTATTTGAAAGTGATGAT+GGG 0.557864 tig0026647:+11182 Msa1430290:intron
AGTTCCACAGGTTGTAAGCA+AGG 0.560370 tig0026647:+13440 Msa1430290:CDS
CTATCCTTGCTTACAACCTG+TGG 0.562710 tig0026647:-13444 None:intergenic
ATAGATACCTTAGACAAGAA+TGG 0.563801 tig0026647:-11719 None:intergenic
AATTGTGTACAAACGGCCAC+CGG 0.564192 tig0026647:+13389 Msa1430290:CDS
TCTTCAGGAAAAGGTGGTGG+TGG 0.564320 tig0026647:-11035 None:intergenic
GTCATTGCCTGGTGGGAAAG+CGG 0.565959 tig0026647:+11598 Msa1430290:CDS
GAATAAATCAAATGGAAAAG+GGG 0.577318 tig0026647:+13656 Msa1430290:three_prime_UTR
TGAAATGATAAGCAATAAGA+TGG 0.579545 tig0026647:+13749 Msa1430290:three_prime_UTR
CTAAGAGTAGTTCCTGTCAT+CGG 0.582497 tig0026647:+13051 Msa1430290:CDS
GGAGTCAGGAAGAGAGAGAG+TGG 0.584588 tig0026647:+13262 Msa1430290:CDS
GTAGGTGAAGTGTCATTGCC+TGG 0.586937 tig0026647:+11587 Msa1430290:intron
AGGATAGTTCAATGACTTGA+CGG 0.587268 tig0026647:+13460 Msa1430290:CDS
CGCGGCCACTGCAAAACGAG+AGG 0.591310 tig0026647:+11640 Msa1430290:CDS
ACATCTTCAGGAAAAGGTGG+TGG 0.591697 tig0026647:-11038 None:intergenic
TCCAAGCTCTCTACTCACTC+AGG 0.594205 tig0026647:+11235 Msa1430290:CDS
TGACAGGAACTACTCTTAGG+AGG 0.607814 tig0026647:-13047 None:intergenic
TTTATTTGAAAGTGATGATG+GGG 0.614807 tig0026647:+11183 Msa1430290:intron
AAAACATCTTCAGGAAAAGG+TGG 0.635918 tig0026647:-11041 None:intergenic
GAACAGAGCATTGAAGAGAG+TGG 0.639203 tig0026647:+11065 Msa1430290:CDS
TTCTGCTCTATGAAAGCCGG+TGG 0.643205 tig0026647:-13405 None:intergenic
AGATCCACCAGTGGTAACGT+TGG 0.652080 tig0026647:-10970 None:intergenic
GTGAAGTGTCATTGCCTGGT+GGG 0.658192 tig0026647:+11591 Msa1430290:CDS
ATTGCCTGGTGGGAAAGCGG+AGG 0.658525 tig0026647:+11601 Msa1430290:CDS
CAGAGCATTGAAGAGAGTGG+TGG 0.680698 tig0026647:+11068 Msa1430290:CDS
GGTGAAGTGTCATTGCCTGG+TGG 0.686704 tig0026647:+11590 Msa1430290:intron
TCATTAGATATCCAAACACA+AGG 0.698944 tig0026647:-13627 None:intergenic
GGAAGGGGATAAGGACGACG+CGG 0.736631 tig0026647:+11622 Msa1430290:CDS
AAGTCTCTGAGATCCACCAG+TGG 0.760789 tig0026647:-10979 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATCATTTTAAGAAAA+GGG - tig0026647:12278-12297 None:intergenic 10.0%
!!! TTATAAATCATTTTAAGAAA+AGG - tig0026647:12279-12298 None:intergenic 10.0%
!!! TTTTCTTTAATAGGAAAAAA+AGG - tig0026647:12431-12450 None:intergenic 15.0%
!!! AATCAATTTGAGAAATTTTT+CGG + tig0026647:12747-12766 Msa1430290:intron 15.0%
!!! TTTTTAGGACTAAAATTATC+TGG - tig0026647:11146-11165 None:intergenic 20.0%
!!! TTTTTTTAGACAAATGTTAG+TGG + tig0026647:11338-11357 Msa1430290:intron 20.0%
!!! TACTTTAGTTATTGTGATTT+TGG + tig0026647:11509-11528 Msa1430290:intron 20.0%
!!! TTTTGGTTATTTGAATTTAG+AGG + tig0026647:11526-11545 Msa1430290:intron 20.0%
!! TTTGGTTATTTGAATTTAGA+GGG + tig0026647:11527-11546 Msa1430290:intron 20.0%
!!! TTTCAGCAATTTTCTTTAAT+AGG - tig0026647:12440-12459 None:intergenic 20.0%
!!! TTTTTCGGAAATTTATTTGT+TGG + tig0026647:12762-12781 Msa1430290:intron 20.0%
!!! GAAATTTATTTGTTGGATAA+TGG + tig0026647:12769-12788 Msa1430290:intron 20.0%
!! GGGTTATAAATTTGTATTAT+TGG + tig0026647:12882-12901 Msa1430290:intron 20.0%
!! TGAAGAAAAGAAATATATTG+AGG - tig0026647:13510-13529 None:intergenic 20.0%
!! TACAATAATATATATTCGTC+AGG + tig0026647:13533-13552 Msa1430290:three_prime_UTR 20.0%
!! TAGAATAAATCAAATGGAAA+AGG + tig0026647:13654-13673 Msa1430290:three_prime_UTR 20.0%
!! AGAATAAATCAAATGGAAAA+GGG + tig0026647:13655-13674 Msa1430290:three_prime_UTR 20.0%
!!! AAAAAATTCCAGACTAATTT+TGG + tig0026647:13683-13702 Msa1430290:three_prime_UTR 20.0%
!!! AAAAATTCCAGACTAATTTT+GGG + tig0026647:13684-13703 Msa1430290:three_prime_UTR 20.0%
!! GGAATAATAAAATGTTGTAA+AGG + tig0026647:13711-13730 Msa1430290:three_prime_UTR 20.0%
!! ATTATCTGGATTTTGAATCA+TGG - tig0026647:11132-11151 None:intergenic 25.0%
! TGTTTATTTGAAAGTGATGA+TGG + tig0026647:11181-11200 Msa1430290:intron 25.0%
! GTTTATTTGAAAGTGATGAT+GGG + tig0026647:11182-11201 Msa1430290:intron 25.0%
!! TTTATTTGAAAGTGATGATG+GGG + tig0026647:11183-11202 Msa1430290:intron 25.0%
! TAAAGATTCAAAATTCCAAG+TGG - tig0026647:11796-11815 None:intergenic 25.0%
!!! CTAGAAAATGTTGATTTTGT+TGG + tig0026647:11864-11883 Msa1430290:intron 25.0%
!! TGTAATTATTTAGCTCATGA+AGG + tig0026647:11907-11926 Msa1430290:intron 25.0%
! ACTTCATTCTTGAATATCAT+CGG + tig0026647:12049-12068 Msa1430290:intron 25.0%
! AATTCCTGAAAACAATAAAC+AGG - tig0026647:12314-12333 None:intergenic 25.0%
! TAACAACACTTCATAGATTT+AGG + tig0026647:12662-12681 Msa1430290:intron 25.0%
! GATACAAATAAAACTAAGCA+TGG - tig0026647:13171-13190 None:intergenic 25.0%
! AGCAGAAAAAATATCTCATA+TGG + tig0026647:13331-13350 Msa1430290:CDS 25.0%
! GCAGAAAAAATATCTCATAT+GGG + tig0026647:13332-13351 Msa1430290:CDS 25.0%
! CAGAAAAAATATCTCATATG+GGG + tig0026647:13333-13352 Msa1430290:CDS 25.0%
! ATTTATATCTCCTGAAAGAA+AGG + tig0026647:13562-13581 Msa1430290:three_prime_UTR 25.0%
! GAATAAATCAAATGGAAAAG+GGG + tig0026647:13656-13675 Msa1430290:three_prime_UTR 25.0%
! TGAAATGATAAGCAATAAGA+TGG + tig0026647:13749-13768 Msa1430290:three_prime_UTR 25.0%
!! TTTTTAATCCATAGATGTGA+TGG + tig0026647:13801-13820 Msa1430290:three_prime_UTR 25.0%
! AATCAAAACAGCAGCTTTTT+AGG - tig0026647:11161-11180 None:intergenic 30.0%
AATTTAGAGGGAATGAATTG+AGG + tig0026647:11539-11558 Msa1430290:intron 30.0%
ATTTAGAGGGAATGAATTGA+GGG + tig0026647:11540-11559 Msa1430290:intron 30.0%
!!! GTAACAACATTGACTAGTTT+TGG - tig0026647:11689-11708 None:intergenic 30.0%
ATAGATACCTTAGACAAGAA+TGG - tig0026647:11722-11741 None:intergenic 30.0%
TTAATTGAGTATTCTCCACT+TGG + tig0026647:11778-11797 Msa1430290:intron 30.0%
AAATTTATGTCTGCGATTGA+TGG + tig0026647:11818-11837 Msa1430290:intron 30.0%
CCAAGACAACAATCATAATA+AGG - tig0026647:12161-12180 None:intergenic 30.0%
CCTTATTATGATTGTTGTCT+TGG + tig0026647:12158-12177 Msa1430290:intron 30.0%
! TCTACCTGTTTATTGTTTTC+AGG + tig0026647:12307-12326 Msa1430290:intron 30.0%
! CTAATCAGTTTGTTTACATC+AGG - tig0026647:12341-12360 None:intergenic 30.0%
CTGATGTAAACAAACTGATT+AGG + tig0026647:12339-12358 Msa1430290:intron 30.0%
CTAAGAAATGTTGCATTCAA+TGG + tig0026647:12395-12414 Msa1430290:intron 30.0%
AAAGCATAAGTTCGTTATTG+TGG - tig0026647:12539-12558 None:intergenic 30.0%
! TTATGCTTTGAACTAGATAG+AGG + tig0026647:12550-12569 Msa1430290:intron 30.0%
!!! TGCATAAAGTTTTGAAACTG+TGG - tig0026647:12795-12814 None:intergenic 30.0%
GAGGAATAGACTAATAGAAA+AGG - tig0026647:12948-12967 None:intergenic 30.0%
GCTGTAAAAGAAACAATGAT+AGG - tig0026647:13026-13045 None:intergenic 30.0%
! ACCTTTAGAAATGTTTCTCA+AGG + tig0026647:13143-13162 Msa1430290:CDS 30.0%
ACCTTGAGAAACATTTCTAA+AGG - tig0026647:13147-13166 None:intergenic 30.0%
GAAATATGAATTCCAAGTGA+TGG - tig0026647:13601-13620 None:intergenic 30.0%
TCATTAGATATCCAAACACA+AGG - tig0026647:13630-13649 None:intergenic 30.0%
GTGTTTGGATATCTAATGAT+CGG + tig0026647:13631-13650 Msa1430290:three_prime_UTR 30.0%
GATCGGTAGAATAAATCAAA+TGG + tig0026647:13648-13667 Msa1430290:three_prime_UTR 30.0%
AAGATGGTGAAAAATGAACT+TGG + tig0026647:13765-13784 Msa1430290:three_prime_UTR 30.0%
AAAACATCTTCAGGAAAAGG+TGG - tig0026647:11044-11063 None:intergenic 35.0%
! ACCTTTTCCTGAAGATGTTT+TGG + tig0026647:11043-11062 Msa1430290:CDS 35.0%
TCCAAAACATCTTCAGGAAA+AGG - tig0026647:11047-11066 None:intergenic 35.0%
!! TGGCAAAGTTGTTTCTCAAT+TGG + tig0026647:11088-11107 Msa1430290:CDS 35.0%
!! GGCAAAGTTGTTTCTCAATT+GGG + tig0026647:11089-11108 Msa1430290:CDS 35.0%
GCACATACACTTCTCATATT+AGG - tig0026647:11410-11429 None:intergenic 35.0%
AACCATTTGCCCAATAGTAT+AGG - tig0026647:11471-11490 None:intergenic 35.0%
ATACTATTGGGCAAATGGTT+TGG + tig0026647:11471-11490 Msa1430290:intron 35.0%
TACTATTGGGCAAATGGTTT+GGG + tig0026647:11472-11491 Msa1430290:intron 35.0%
TGGTGTGTGTGTTTATATGT+AGG + tig0026647:11569-11588 Msa1430290:intron 35.0%
TCTTGAACCATTCTTGTCTA+AGG + tig0026647:11712-11731 Msa1430290:CDS 35.0%
GTCTGTATAAAGTAATGCAG+TGG + tig0026647:12206-12225 Msa1430290:intron 35.0%
GGGTATGTATCTGATTCTTT+AGG - tig0026647:12258-12277 None:intergenic 35.0%
! TACATGACTTTTACAGTTGG+TGG - tig0026647:12477-12496 None:intergenic 35.0%
! GGATACATGACTTTTACAGT+TGG - tig0026647:12480-12499 None:intergenic 35.0%
AAAAGTCATGTATCCATTGC+AGG + tig0026647:12485-12504 Msa1430290:intron 35.0%
ACGTTCTTATTAGCAACGAT+TGG + tig0026647:12717-12736 Msa1430290:intron 35.0%
AAGTATGACCATTTCAGAGT+CGG + tig0026647:12848-12867 Msa1430290:intron 35.0%
ATGTTTACATTCCCTGTTGT+TGG + tig0026647:12910-12929 Msa1430290:intron 35.0%
GTGTAAACTATCCAACAACA+GGG - tig0026647:12924-12943 None:intergenic 35.0%
TGTGTAAACTATCCAACAAC+AGG - tig0026647:12925-12944 None:intergenic 35.0%
! GCTTTAAGCAGGATGAAAAT+CGG + tig0026647:13241-13260 Msa1430290:intron 35.0%
CTGCATCAATTGTGTACAAA+CGG + tig0026647:13382-13401 Msa1430290:CDS 35.0%
ACCTGTGGAACTTTGAATTT+GGG - tig0026647:13432-13451 None:intergenic 35.0%
AACCTGTGGAACTTTGAATT+TGG - tig0026647:13433-13452 None:intergenic 35.0%
AGGATAGTTCAATGACTTGA+CGG + tig0026647:13460-13479 Msa1430290:CDS 35.0%
CTCTGTTCCAAAACATCTTC+AGG - tig0026647:11053-11072 None:intergenic 40.0%
! TGAATCATGGTAGCTGATTC+AGG - tig0026647:11119-11138 None:intergenic 40.0%
!! AAAGTGATGATGGGGATCTA+AGG + tig0026647:11191-11210 Msa1430290:intron 40.0%
!! AAGTGATGATGGGGATCTAA+GGG + tig0026647:11192-11211 Msa1430290:intron 40.0%
TTTGCCCAATAGTATAGGCT+GGG - tig0026647:11466-11485 None:intergenic 40.0%
ATTTGCCCAATAGTATAGGC+TGG - tig0026647:11467-11486 None:intergenic 40.0%
AGCCTATACTATTGGGCAAA+TGG + tig0026647:11466-11485 Msa1430290:intron 40.0%
ACTATTGGGCAAATGGTTTG+GGG + tig0026647:11473-11492 Msa1430290:intron 40.0%
! TGCGATTGATGGTTAGTTCA+TGG + tig0026647:11829-11848 Msa1430290:intron 40.0%
TACTTATTCCTCACACAGCA+TGG - tig0026647:11938-11957 None:intergenic 40.0%
TAAAGTAATGCAGTGGCCAT+TGG + tig0026647:12213-12232 Msa1430290:intron 40.0%
TCCATTGCAGGTCATTAAAC+TGG + tig0026647:12497-12516 Msa1430290:intron 40.0%
CCATTGCAGGTCATTAAACT+GGG + tig0026647:12498-12517 Msa1430290:intron 40.0%
ACTTAGTGCTCAGCATAAAC+AGG + tig0026647:12622-12641 Msa1430290:intron 40.0%
AAATTTATAACCCGTGACCG+CGG - tig0026647:12875-12894 None:intergenic 40.0%
AGTCTATTCCTCATCTGCTT+CGG + tig0026647:12956-12975 Msa1430290:intron 40.0%
!! CGGATTGAAAGTGTTCAATG+AGG + tig0026647:12976-12995 Msa1430290:intron 40.0%
TGATAGGTGAGATGAAGTAC+TGG - tig0026647:13010-13029 None:intergenic 40.0%
CTAAGAGTAGTTCCTGTCAT+CGG + tig0026647:13051-13070 Msa1430290:CDS 40.0%
!! AATACTGATTCCACTTCTGC+AGG - tig0026647:13117-13136 None:intergenic 40.0%
!!! TGACTGCCGGAATTTTGATT+AGG + tig0026647:13358-13377 Msa1430290:CDS 40.0%
!!! GACTGCCGGAATTTTGATTA+GGG + tig0026647:13359-13378 Msa1430290:CDS 40.0%
TGCAGCCCTAATCAAAATTC+CGG - tig0026647:13367-13386 None:intergenic 40.0%
TCCCAAATTCAAAGTTCCAC+AGG + tig0026647:13428-13447 Msa1430290:CDS 40.0%
ATCTCCTGAAAGAAAGGAAG+AGG + tig0026647:13568-13587 Msa1430290:three_prime_UTR 40.0%
ATTTCTGCTCACCTTGTGTT+TGG + tig0026647:13616-13635 Msa1430290:three_prime_UTR 40.0%
!!! TCCAGACTAATTTTGGGATC+TGG + tig0026647:13690-13709 Msa1430290:three_prime_UTR 40.0%
TCCAGATCCCAAAATTAGTC+TGG - tig0026647:13694-13713 None:intergenic 40.0%
TCTTCTTCCAACGTTACCAC+TGG + tig0026647:10963-10982 Msa1430290:CDS 45.0%
ATCTCAGAGACTTCTTGCGT+TGG + tig0026647:10989-11008 Msa1430290:CDS 45.0%
ACATCTTCAGGAAAAGGTGG+TGG - tig0026647:11041-11060 None:intergenic 45.0%
!! GAACAGAGCATTGAAGAGAG+TGG + tig0026647:11065-11084 Msa1430290:CDS 45.0%
AGATAACGTGTGTCTGACAC+AGG + tig0026647:11378-11397 Msa1430290:intron 45.0%
GATAACGTGTGTCTGACACA+GGG + tig0026647:11379-11398 Msa1430290:intron 45.0%
TGTGTCCCAGCCTATACTAT+TGG + tig0026647:11458-11477 Msa1430290:intron 45.0%
GTGTCCCAGCCTATACTATT+GGG + tig0026647:11459-11478 Msa1430290:intron 45.0%
AGGGAATGAATTGAGGGTTG+TGG + tig0026647:11546-11565 Msa1430290:intron 45.0%
GAATGAATTGAGGGTTGTGG+TGG + tig0026647:11549-11568 Msa1430290:intron 45.0%
GAGAGGCAGAAGAGGAAATT+GGG + tig0026647:11657-11676 Msa1430290:CDS 45.0%
! TGCTCTGAGTCTGAGATGAT+TGG + tig0026647:11965-11984 Msa1430290:intron 45.0%
! GAGTCTGAGATGATTGGAGA+TGG + tig0026647:11971-11990 Msa1430290:intron 45.0%
! TCTGAGATGATTGGAGATGG+AGG + tig0026647:11974-11993 Msa1430290:intron 45.0%
CTGAGATGATTGGAGATGGA+GGG + tig0026647:11975-11994 Msa1430290:intron 45.0%
TCAGCTGCACTTCATGTATC+TGG - tig0026647:12114-12133 None:intergenic 45.0%
AGATACATGAAGTGCAGCTG+AGG + tig0026647:12113-12132 Msa1430290:intron 45.0%
ATCCCATTCCCATTCACCAA+TGG - tig0026647:12232-12251 None:intergenic 45.0%
TTCTTCACTGTCCACGTTCA+TGG + tig0026647:12363-12382 Msa1430290:intron 45.0%
AGTACGTACAACCATGAACG+TGG - tig0026647:12377-12396 None:intergenic 45.0%
CCCAGTTTAATGACCTGCAA+TGG - tig0026647:12501-12520 None:intergenic 45.0%
CTTTCAATCCGAAGCAGATG+AGG - tig0026647:12967-12986 None:intergenic 45.0%
TGACAGGAACTACTCTTAGG+AGG - tig0026647:13050-13069 None:intergenic 45.0%
CGATGACAGGAACTACTCTT+AGG - tig0026647:13053-13072 None:intergenic 45.0%
TCATGAAGTATGCCGATGAC+AGG - tig0026647:13066-13085 None:intergenic 45.0%
ACTTCTGCAGGATTCAGAAC+AGG - tig0026647:13105-13124 None:intergenic 45.0%
! TGTTCTGAATCCTGCAGAAG+TGG + tig0026647:13104-13123 Msa1430290:CDS 45.0%
! CTGAAGCGATTGCTTTAAGC+AGG + tig0026647:13230-13249 Msa1430290:intron 45.0%
AATTGTGTACAAACGGCCAC+CGG + tig0026647:13389-13408 Msa1430290:CDS 45.0%
GGATTCTGCTCTATGAAAGC+CGG - tig0026647:13411-13430 None:intergenic 45.0%
AGTTCCACAGGTTGTAAGCA+AGG + tig0026647:13440-13459 Msa1430290:CDS 45.0%
CTATCCTTGCTTACAACCTG+TGG - tig0026647:13447-13466 None:intergenic 45.0%
GCATCCTCTTCCTTTCTTTC+AGG - tig0026647:13575-13594 None:intergenic 45.0%
GCAAACTGCCATCACATCTA+TGG - tig0026647:13812-13831 None:intergenic 45.0%
TCTTCCAACGTTACCACTGG+TGG + tig0026647:10966-10985 Msa1430290:CDS 50.0%
! AGATCCACCAGTGGTAACGT+TGG - tig0026647:10973-10992 None:intergenic 50.0%
!! AAGTCTCTGAGATCCACCAG+TGG - tig0026647:10982-11001 None:intergenic 50.0%
! TCTTCAGGAAAAGGTGGTGG+TGG - tig0026647:11038-11057 None:intergenic 50.0%
!! CAGAGCATTGAAGAGAGTGG+TGG + tig0026647:11068-11087 Msa1430290:CDS 50.0%
TCCAAGCTCTCTACTCACTC+AGG + tig0026647:11235-11254 Msa1430290:CDS 50.0%
ACCTGAGTGAGTAGAGAGCT+TGG - tig0026647:11239-11258 None:intergenic 50.0%
! GTAGGTGAAGTGTCATTGCC+TGG + tig0026647:11587-11606 Msa1430290:intron 50.0%
! GTGAAGTGTCATTGCCTGGT+GGG + tig0026647:11591-11610 Msa1430290:CDS 50.0%
! TTCTGCCTCTCGTTTTGCAG+TGG - tig0026647:11648-11667 None:intergenic 50.0%
TGCAAAACGAGAGGCAGAAG+AGG + tig0026647:11649-11668 Msa1430290:CDS 50.0%
CGAGAGGCAGAAGAGGAAAT+TGG + tig0026647:11656-11675 Msa1430290:CDS 50.0%
GGCAGAAGAGGAAATTGGGT+TGG + tig0026647:11661-11680 Msa1430290:CDS 50.0%
AGGAGATTCCATGCTGTGTG+AGG + tig0026647:11927-11946 Msa1430290:intron 50.0%
ATGCAGTGGCCATTGGTGAA+TGG + tig0026647:12220-12239 Msa1430290:intron 50.0%
TGCAGTGGCCATTGGTGAAT+GGG + tig0026647:12221-12240 Msa1430290:intron 50.0%
TGGCCATTGGTGAATGGGAA+TGG + tig0026647:12226-12245 Msa1430290:intron 50.0%
GGCCATTGGTGAATGGGAAT+GGG + tig0026647:12227-12246 Msa1430290:intron 50.0%
GCAGGATGAAAATCGGAGTC+AGG + tig0026647:13248-13267 Msa1430290:intron 50.0%
TCAGGAAGAGAGAGAGTGGA+TGG + tig0026647:13266-13285 Msa1430290:CDS 50.0%
CAGGAAGAGAGAGAGTGGAT+GGG + tig0026647:13267-13286 Msa1430290:CDS 50.0%
CTCATATGGGGATTGACTGC+CGG + tig0026647:13345-13364 Msa1430290:CDS 50.0%
TTCTGCTCTATGAAAGCCGG+TGG - tig0026647:13408-13427 None:intergenic 50.0%
AGAGGATGCTGTCCATCACT+TGG + tig0026647:13586-13605 Msa1430290:three_prime_UTR 50.0%
! GGTGAAGTGTCATTGCCTGG+TGG + tig0026647:11590-11609 Msa1430290:intron 55.0%
GTCATTGCCTGGTGGGAAAG+CGG + tig0026647:11598-11617 Msa1430290:CDS 55.0%
GAAAGCGGAGGAAGGGGATA+AGG + tig0026647:11613-11632 Msa1430290:CDS 55.0%
!! GAAGTGCAGCTGAGGTACTG+AGG + tig0026647:12121-12140 Msa1430290:intron 55.0%
!! ACAGTTGGTGGCACGAGCAA+TGG - tig0026647:12465-12484 None:intergenic 55.0%
ACCATTTCAGAGTCGGTCCG+CGG + tig0026647:12855-12874 Msa1430290:intron 55.0%
GGAGTCAGGAAGAGAGAGAG+TGG + tig0026647:13262-13281 Msa1430290:CDS 55.0%
ATTGCCTGGTGGGAAAGCGG+AGG + tig0026647:11601-11620 Msa1430290:CDS 60.0%
CTGGTGGGAAAGCGGAGGAA+GGG + tig0026647:11606-11625 Msa1430290:CDS 60.0%
TGGTGGGAAAGCGGAGGAAG+GGG + tig0026647:11607-11626 Msa1430290:CDS 60.0%
GGAAGGGGATAAGGACGACG+CGG + tig0026647:11622-11641 Msa1430290:CDS 60.0%
! ACCGCGGACCGACTCTGAAA+TGG - tig0026647:12859-12878 None:intergenic 60.0%
CCTTCCTCCGCTTTCCCACC+AGG - tig0026647:11608-11627 None:intergenic 65.0%
CCTGGTGGGAAAGCGGAGGA+AGG + tig0026647:11605-11624 Msa1430290:CDS 65.0%
CGCGGCCACTGCAAAACGAG+AGG + tig0026647:11640-11659 Msa1430290:CDS 65.0%
TCAGAGTCGGTCCGCGGTCA+CGG + tig0026647:12861-12880 Msa1430290:intron 65.0%
CAGAGTCGGTCCGCGGTCAC+GGG + tig0026647:12862-12881 Msa1430290:intron 70.0%
Chromosome Type Strat End Strand Name
tig0026647 gene 10957 13860 10957 ID=Msa1430290;Name=Msa1430290
tig0026647 mRNA 10957 13860 10957 ID=Msa1430290-mRNA-1;Parent=Msa1430290;Name=Msa1430290-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.75|0.6|1|1|1|5|381|255
tig0026647 exon 10957 11171 10957 ID=Msa1430290-mRNA-1:exon:6951;Parent=Msa1430290-mRNA-1
tig0026647 exon 11195 11256 11195 ID=Msa1430290-mRNA-1:exon:6952;Parent=Msa1430290-mRNA-1
tig0026647 exon 11591 11733 11591 ID=Msa1430290-mRNA-1:exon:6953;Parent=Msa1430290-mRNA-1
tig0026647 exon 13045 13164 13045 ID=Msa1430290-mRNA-1:exon:6954;Parent=Msa1430290-mRNA-1
tig0026647 exon 13252 13860 13252 ID=Msa1430290-mRNA-1:exon:6955;Parent=Msa1430290-mRNA-1
tig0026647 CDS 10957 11171 10957 ID=Msa1430290-mRNA-1:cds;Parent=Msa1430290-mRNA-1
tig0026647 CDS 11195 11256 11195 ID=Msa1430290-mRNA-1:cds;Parent=Msa1430290-mRNA-1
tig0026647 CDS 11591 11733 11591 ID=Msa1430290-mRNA-1:cds;Parent=Msa1430290-mRNA-1
tig0026647 CDS 13045 13164 13045 ID=Msa1430290-mRNA-1:cds;Parent=Msa1430290-mRNA-1
tig0026647 CDS 13252 13479 13252 ID=Msa1430290-mRNA-1:cds;Parent=Msa1430290-mRNA-1
tig0026647 three_prime_UTR 13480 13860 13480 ID=Msa1430290-mRNA-1:three_prime_utr;Parent=Msa1430290-mRNA-1
Gene Sequence

>Msa1430290

ATGGATTCTTCTTCCAACGTTACCACTGGTGGATCTCAGAGACTTCTTGCGTTGGCACAACATCTTCGTCTCTACAAGCCACCACCACCTTTTCCTGAAGATGTTTTGGAACAGAGCATTGAAGAGAGTGGTGGCAAAGTTGTTTCTCAATTGGGTTTTCCTGAATCAGCTACCATGATTCAAAATCCAGATAATTTTAGTCCTAAAAAGCTGCTTGATGATGGGGATCTAAGGGTTATTCTCACTAAACGATCTTCCAAGCTCTCTACTCACTCAGGTGAAGTGTCATTGCCTGGTGGGAAAGCGGAGGAAGGGGATAAGGACGACGCGGCCACTGCAAAACGAGAGGCAGAAGAGGAAATTGGGTTGGATCCAAAACTAGTCAATGTTGTTACTGTTCTTGAACCATTCTTGTCTAAGCACCTCCTAAGAGTAGTTCCTGTCATCGGCATACTTCATGATAAGAAAGCGTTCAAACCTGTTCTGAATCCTGCAGAAGTGGAATCAGTATTTGACGCACCTTTAGAAATGTTTCTCAAGGATGAAAATCGGAGTCAGGAAGAGAGAGAGTGGATGGGAGAGAAGTATTTGATACATTTTTTTGACTATATTGACATCGAGCAGAAAAAATATCTCATATGGGGATTGACTGCCGGAATTTTGATTAGGGCTGCATCAATTGTGTACAAACGGCCACCGGCTTTCATAGAGCAGAATCCCAAATTCAAAGTTCCACAGGTTGTAAGCAAGGATAGTTCAATGACTTGA
Protein sequence

>Msa1430290

MDSSSNVTTGGSQRLLALAQHLRLYKPPPPFPEDVLEQSIEESGGKVVSQLGFPESATMIQNPDNFSPKKLLDDGDLRVILTKRSSKLSTHSGEVSLPGGKAEEGDKDDAATAKREAEEEIGLDPKLVNVVTVLEPFLSKHLLRVVPVIGILHDKKAFKPVLNPAEVESVFDAPLEMFLKDENRSQEEREWMGEKYLIHFFDYIDIEQKKYLIWGLTAGILIRAASIVYKRPPAFIEQNPKFKVPQVVSKDSSMT*