AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0695010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0695010 MtrunA17_Chr4g0046091 96.154 286 10 1 1 285 1 286 0.0 558
Msa0695010 MtrunA17_Chr2g0283341 49.145 234 90 4 29 262 1 205 5.81e-74 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0695010 AT1G28960.3 69.825 285 81 4 3 284 10 292 1.22e-132 377
Msa0695010 AT1G28960.1 69.825 285 81 4 3 284 10 292 1.22e-132 377
Msa0695010 AT1G28960.5 69.825 285 81 4 3 284 10 292 1.22e-132 377
Msa0695010 AT1G28960.7 70.504 278 77 4 3 277 10 285 1.30e-130 372
Msa0695010 AT1G28960.6 70.504 278 77 4 3 277 10 285 1.30e-130 372
Msa0695010 AT2G33980.2 68.016 247 72 3 33 277 18 259 3.12e-114 330
Msa0695010 AT2G33980.4 68.016 247 72 3 33 277 18 259 3.12e-114 330
Msa0695010 AT2G33980.1 68.016 247 72 3 33 277 18 259 1.33e-113 330
Msa0695010 AT2G33980.3 68.016 247 72 3 33 277 18 259 3.52e-113 329
Msa0695010 AT5G45940.1 61.053 190 70 2 87 272 28 217 2.81e-79 239
Msa0695010 AT5G45940.2 62.791 129 45 1 87 212 28 156 1.70e-51 166
Msa0695010 AT5G45940.3 63.780 127 43 1 87 210 28 154 5.19e-51 166

Find 98 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCCTCTTCCTTTCTTTC+AGG 0.144264 4_4:-64418507 None:intergenic
AACCTGTGGAACTTTGAATT+TGG 0.148694 4_4:-64418365 None:intergenic
TGAATCATGGTAGCTGATTC+AGG 0.246118 4_4:-64416046 None:intergenic
GTAACAACATTGACTAGTTT+TGG 0.246703 4_4:-64416618 None:intergenic
CGAGAGGCAGAAGAGGAAAT+TGG 0.270083 4_4:+64416588 Msa0695010:CDS
TCTTGAACCATTCTTGTCTA+AGG 0.274255 4_4:+64416644 Msa0695010:CDS
CTCTGTTCCAAAACATCTTC+AGG 0.287071 4_4:-64415980 None:intergenic
ATTTCTGCTCACCTTGTGTT+TGG 0.302067 4_4:+64418551 Msa0695010:three_prime_UTR
TGGCAAAGTTGTTTCTCAAT+TGG 0.304222 4_4:+64416018 Msa0695010:CDS
ACCTGTGGAACTTTGAATTT+GGG 0.326845 4_4:-64418364 None:intergenic
GCAAACTGCCATCACATCTA+TGG 0.329294 4_4:-64418744 None:intergenic
AAAGTGATGATGGGGATCTA+AGG 0.334445 4_4:+64416122 Msa0695010:CDS
GGCAAAGTTGTTTCTCAATT+GGG 0.351294 4_4:+64416019 Msa0695010:CDS
CAATAACAATTTGATACTAT+AGG 0.355004 4_4:+64415747 Msa0695010:five_prime_UTR
TTGGTGACAGATACTGTTCT+TGG 0.364656 4_4:-64415860 None:intergenic
ACTAGCAGTTTAATGTTTGA+AGG 0.371560 4_4:-64415687 None:intergenic
TAGAATAAATCAAATGGAAA+AGG 0.394241 4_4:+64418589 Msa0695010:three_prime_UTR
CGATGACAGGAACTACTCTT+AGG 0.397188 4_4:-64417985 None:intergenic
AATAACAATTTGATACTATA+GGG 0.401857 4_4:+64415748 Msa0695010:five_prime_UTR
TCCAGATCCCAAAATTAGTC+TGG 0.402451 4_4:-64418626 None:intergenic
TGCAGCCCTAATCAAAATTC+CGG 0.409951 4_4:-64418299 None:intergenic
TCAGGAAGAGAGAGAGTGGA+TGG 0.414184 4_4:+64418201 Msa0695010:CDS
TGCTAGTTTAACTACTTGTC+CGG 0.424659 4_4:+64415703 Msa0695010:five_prime_UTR
CCTTCCTCCGCTTTCCCACC+AGG 0.426104 4_4:-64416537 None:intergenic
TACAATAATATATATTCGTC+AGG 0.427184 4_4:+64418468 Msa0695010:three_prime_UTR
AGAATAAATCAAATGGAAAA+GGG 0.430085 4_4:+64418590 Msa0695010:three_prime_UTR
TGTTTATTTGAAAGTGATGA+TGG 0.443684 4_4:+64416112 Msa0695010:CDS
TTGCAAAAGCTAATCTAAAC+CGG 0.444277 4_4:-64415722 None:intergenic
GAAAGCGGAGGAAGGGGATA+AGG 0.452799 4_4:+64416545 Msa0695010:CDS
GATCGGTAGAATAAATCAAA+TGG 0.459510 4_4:+64418583 Msa0695010:three_prime_UTR
ACCTGAGTGAGTAGAGAGCT+TGG 0.464566 4_4:-64416167 None:intergenic
AATACTGATTCCACTTCTGC+AGG 0.466410 4_4:-64418049 None:intergenic
ACTTCTGCAGGATTCAGAAC+AGG 0.470641 4_4:-64418037 None:intergenic
TGGTGGGAAAGCGGAGGAAG+GGG 0.473889 4_4:+64416539 Msa0695010:CDS
GCTTTAAGCAGGATGAAAAT+CGG 0.478548 4_4:+64418176 Msa0695010:intron
TTTGAAGGTCTGATCCTTGT+CGG 0.479051 4_4:-64415672 None:intergenic
CCTGGTGGGAAAGCGGAGGA+AGG 0.480967 4_4:+64416537 Msa0695010:CDS
TCCAAAACATCTTCAGGAAA+AGG 0.484289 4_4:-64415974 None:intergenic
GGAATAATAAAATGTTGTAA+AGG 0.484974 4_4:+64418646 Msa0695010:three_prime_UTR
ATTTATATCTCCTGAAAGAA+AGG 0.485574 4_4:+64418497 Msa0695010:three_prime_UTR
GGAAGAAGAATCCATGACTT+TGG 0.487067 4_4:-64415879 None:intergenic
GAAATATGAATTCCAAGTGA+TGG 0.489702 4_4:-64418533 None:intergenic
GAGAGGCAGAAGAGGAAATT+GGG 0.494487 4_4:+64416589 Msa0695010:CDS
TGTTCTGAATCCTGCAGAAG+TGG 0.496762 4_4:+64418039 Msa0695010:CDS
CTGGTGGGAAAGCGGAGGAA+GGG 0.497359 4_4:+64416538 Msa0695010:CDS
GCAGGATGAAAATCGGAGTC+AGG 0.500021 4_4:+64418183 Msa0695010:intron
ACCTTGAGAAACATTTCTAA+AGG 0.503154 4_4:-64418079 None:intergenic
TCTTCCAACGTTACCACTGG+TGG 0.507992 4_4:+64415896 Msa0695010:CDS
TCCCAAATTCAAAGTTCCAC+AGG 0.509189 4_4:+64418363 Msa0695010:CDS
CTGCATCAATTGTGTACAAA+CGG 0.513281 4_4:+64418317 Msa0695010:CDS
CTCATATGGGGATTGACTGC+CGG 0.519065 4_4:+64418280 Msa0695010:CDS
ATCTCCTGAAAGAAAGGAAG+AGG 0.524063 4_4:+64418503 Msa0695010:three_prime_UTR
GGCAGAAGAGGAAATTGGGT+TGG 0.528513 4_4:+64416593 Msa0695010:CDS
TCATGAAGTATGCCGATGAC+AGG 0.534330 4_4:-64417998 None:intergenic
TCTTCTTCCAACGTTACCAC+TGG 0.535311 4_4:+64415893 Msa0695010:CDS
AGAGGATGCTGTCCATCACT+TGG 0.536088 4_4:+64418521 Msa0695010:three_prime_UTR
CAGGAAGAGAGAGAGTGGAT+GGG 0.539082 4_4:+64418202 Msa0695010:CDS
AAGTGATGATGGGGATCTAA+GGG 0.540717 4_4:+64416123 Msa0695010:CDS
TGAAGAAAAGAAATATATTG+AGG 0.545140 4_4:-64418442 None:intergenic
GTGTTTGGATATCTAATGAT+CGG 0.547839 4_4:+64418566 Msa0695010:three_prime_UTR
ACCTTTAGAAATGTTTCTCA+AGG 0.548045 4_4:+64418078 Msa0695010:CDS
GGATTCTGCTCTATGAAAGC+CGG 0.548477 4_4:-64418343 None:intergenic
TGCAAAACGAGAGGCAGAAG+AGG 0.549211 4_4:+64416581 Msa0695010:CDS
ATCTCAGAGACTTCTTGCGT+TGG 0.549765 4_4:+64415919 Msa0695010:CDS
ATTTGATACTATAGGGTAGG+TGG 0.554437 4_4:+64415755 Msa0695010:five_prime_UTR
GTTTATTTGAAAGTGATGAT+GGG 0.557864 4_4:+64416113 Msa0695010:CDS
AGTTCCACAGGTTGTAAGCA+AGG 0.560370 4_4:+64418375 Msa0695010:CDS
CTATCCTTGCTTACAACCTG+TGG 0.562710 4_4:-64418379 None:intergenic
ATAGATACCTTAGACAAGAA+TGG 0.563801 4_4:-64416651 None:intergenic
AATTGTGTACAAACGGCCAC+CGG 0.564192 4_4:+64418324 Msa0695010:CDS
TCTTCAGGAAAAGGTGGTGG+TGG 0.564320 4_4:-64415965 None:intergenic
GTCATTGCCTGGTGGGAAAG+CGG 0.565959 4_4:+64416530 Msa0695010:CDS
GAATAAATCAAATGGAAAAG+GGG 0.577318 4_4:+64418591 Msa0695010:three_prime_UTR
TGAAATGATAAGCAATAAGA+TGG 0.579545 4_4:+64418684 Msa0695010:three_prime_UTR
AGTATCTGTCACCAAAGTCA+TGG 0.580073 4_4:+64415868 Msa0695010:CDS
CTAAGAGTAGTTCCTGTCAT+CGG 0.582497 4_4:+64417986 Msa0695010:CDS
GGAGTCAGGAAGAGAGAGAG+TGG 0.584588 4_4:+64418197 Msa0695010:CDS
GTAGGTGAAGTGTCATTGCC+TGG 0.586937 4_4:+64416519 Msa0695010:intron
AGGATAGTTCAATGACTTGA+CGG 0.587268 4_4:+64418395 Msa0695010:CDS
CGCGGCCACTGCAAAACGAG+AGG 0.591310 4_4:+64416572 Msa0695010:CDS
ACATCTTCAGGAAAAGGTGG+TGG 0.591697 4_4:-64415968 None:intergenic
TCCAAGCTCTCTACTCACTC+AGG 0.594205 4_4:+64416166 Msa0695010:CDS
ACAATTTGATACTATAGGGT+AGG 0.599534 4_4:+64415752 Msa0695010:five_prime_UTR
TTTGATACTATAGGGTAGGT+GGG 0.601183 4_4:+64415756 Msa0695010:five_prime_UTR
TGACAGGAACTACTCTTAGG+AGG 0.607814 4_4:-64417982 None:intergenic
TTTATTTGAAAGTGATGATG+GGG 0.614807 4_4:+64416114 Msa0695010:CDS
AAAACATCTTCAGGAAAAGG+TGG 0.635918 4_4:-64415971 None:intergenic
GAACAGAGCATTGAAGAGAG+TGG 0.639203 4_4:+64415995 Msa0695010:CDS
TTCTGCTCTATGAAAGCCGG+TGG 0.643205 4_4:-64418340 None:intergenic
AGATCCACCAGTGGTAACGT+TGG 0.652080 4_4:-64415900 None:intergenic
GTGAAGTGTCATTGCCTGGT+GGG 0.658192 4_4:+64416523 Msa0695010:CDS
ATTGCCTGGTGGGAAAGCGG+AGG 0.658525 4_4:+64416533 Msa0695010:CDS
ACAAAGATTTCATTCCGACA+AGG 0.669608 4_4:+64415658 Msa0695010:five_prime_UTR
CAGAGCATTGAAGAGAGTGG+TGG 0.680698 4_4:+64415998 Msa0695010:CDS
GGTGAAGTGTCATTGCCTGG+TGG 0.686704 4_4:+64416522 Msa0695010:intron
TCATTAGATATCCAAACACA+AGG 0.698944 4_4:-64418562 None:intergenic
GGAAGGGGATAAGGACGACG+CGG 0.736631 4_4:+64416554 Msa0695010:CDS
AAGTCTCTGAGATCCACCAG+TGG 0.760789 4_4:-64415909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATCATTTTAAGAAAA+GGG - chr4_4:64417211-64417230 None:intergenic 10.0%
!!! TTATAAATCATTTTAAGAAA+AGG - chr4_4:64417212-64417231 None:intergenic 10.0%
!! AATAACAATTTGATACTATA+GGG + chr4_4:64415748-64415767 Msa0695010:five_prime_UTR 15.0%
!!! TTTTCTTTAATAGGAAAAAA+AGG - chr4_4:64417364-64417383 None:intergenic 15.0%
!!! AATCAATTTGAGAAATTTTT+CGG + chr4_4:64417680-64417699 Msa0695010:intron 15.0%
!!! TCTTTGTCTTAGATTTTATA+TGG - chr4_4:64415645-64415664 None:intergenic 20.0%
!! CAATAACAATTTGATACTAT+AGG + chr4_4:64415747-64415766 Msa0695010:five_prime_UTR 20.0%
!!! TTTTTAGGACTAAAATTATC+TGG - chr4_4:64416076-64416095 None:intergenic 20.0%
!!! TTTTTTTAGACAAATGTTAG+TGG + chr4_4:64416269-64416288 Msa0695010:intron 20.0%
!!! TACTTTAGTTATTGTGATTT+TGG + chr4_4:64416440-64416459 Msa0695010:intron 20.0%
!!! TTTTGGTTATTTGAATTTAG+AGG + chr4_4:64416457-64416476 Msa0695010:intron 20.0%
!! TTTGGTTATTTGAATTTAGA+GGG + chr4_4:64416458-64416477 Msa0695010:intron 20.0%
!!! TTTCAGCAATTTTCTTTAAT+AGG - chr4_4:64417373-64417392 None:intergenic 20.0%
!!! TTTTTCGGAAATTTATTTGT+TGG + chr4_4:64417695-64417714 Msa0695010:intron 20.0%
!!! GAAATTTATTTGTTGGATAA+TGG + chr4_4:64417702-64417721 Msa0695010:intron 20.0%
!! GGGTTATAAATTTGTATTAT+TGG + chr4_4:64417817-64417836 Msa0695010:intron 20.0%
!! TGAAGAAAAGAAATATATTG+AGG - chr4_4:64418445-64418464 None:intergenic 20.0%
!! TACAATAATATATATTCGTC+AGG + chr4_4:64418468-64418487 Msa0695010:three_prime_UTR 20.0%
!! TAGAATAAATCAAATGGAAA+AGG + chr4_4:64418589-64418608 Msa0695010:three_prime_UTR 20.0%
!! AGAATAAATCAAATGGAAAA+GGG + chr4_4:64418590-64418609 Msa0695010:three_prime_UTR 20.0%
!!! AAAAAATTCCAGACTAATTT+TGG + chr4_4:64418618-64418637 Msa0695010:three_prime_UTR 20.0%
!!! AAAAATTCCAGACTAATTTT+GGG + chr4_4:64418619-64418638 Msa0695010:three_prime_UTR 20.0%
!! GGAATAATAAAATGTTGTAA+AGG + chr4_4:64418646-64418665 Msa0695010:three_prime_UTR 20.0%
!! ATTATCTGGATTTTGAATCA+TGG - chr4_4:64416062-64416081 None:intergenic 25.0%
! TGTTTATTTGAAAGTGATGA+TGG + chr4_4:64416112-64416131 Msa0695010:CDS 25.0%
! GTTTATTTGAAAGTGATGAT+GGG + chr4_4:64416113-64416132 Msa0695010:CDS 25.0%
!! TTTATTTGAAAGTGATGATG+GGG + chr4_4:64416114-64416133 Msa0695010:CDS 25.0%
! TAAAGATTCAAAATTCCAAG+TGG - chr4_4:64416728-64416747 None:intergenic 25.0%
!!! CTAGAAAATGTTGATTTTGT+TGG + chr4_4:64416796-64416815 Msa0695010:intron 25.0%
!! TGTAATTATTTAGCTCATGA+AGG + chr4_4:64416839-64416858 Msa0695010:intron 25.0%
! ACTTCATTCTTGAATATCAT+CGG + chr4_4:64416981-64417000 Msa0695010:intron 25.0%
! AATTCCTGAAAACAATAAAC+AGG - chr4_4:64417247-64417266 None:intergenic 25.0%
! TAACAACACTTCATAGATTT+AGG + chr4_4:64417595-64417614 Msa0695010:intron 25.0%
! GATACAAATAAAACTAAGCA+TGG - chr4_4:64418106-64418125 None:intergenic 25.0%
! AGCAGAAAAAATATCTCATA+TGG + chr4_4:64418266-64418285 Msa0695010:CDS 25.0%
! GCAGAAAAAATATCTCATAT+GGG + chr4_4:64418267-64418286 Msa0695010:CDS 25.0%
! CAGAAAAAATATCTCATATG+GGG + chr4_4:64418268-64418287 Msa0695010:CDS 25.0%
! ATTTATATCTCCTGAAAGAA+AGG + chr4_4:64418497-64418516 Msa0695010:three_prime_UTR 25.0%
! GAATAAATCAAATGGAAAAG+GGG + chr4_4:64418591-64418610 Msa0695010:three_prime_UTR 25.0%
! TGAAATGATAAGCAATAAGA+TGG + chr4_4:64418684-64418703 Msa0695010:three_prime_UTR 25.0%
!! TTTTTAATCCATAGATGTGA+TGG + chr4_4:64418736-64418755 Msa0695010:three_prime_UTR 25.0%
ACTAGCAGTTTAATGTTTGA+AGG - chr4_4:64415690-64415709 None:intergenic 30.0%
TTGCAAAAGCTAATCTAAAC+CGG - chr4_4:64415725-64415744 None:intergenic 30.0%
ACAATTTGATACTATAGGGT+AGG + chr4_4:64415752-64415771 Msa0695010:five_prime_UTR 30.0%
!!! ATCAAAACAGCAGCTTTTTT+AGG - chr4_4:64416091-64416110 None:intergenic 30.0%
AATTTAGAGGGAATGAATTG+AGG + chr4_4:64416470-64416489 Msa0695010:intron 30.0%
ATTTAGAGGGAATGAATTGA+GGG + chr4_4:64416471-64416490 Msa0695010:intron 30.0%
!!! GTAACAACATTGACTAGTTT+TGG - chr4_4:64416621-64416640 None:intergenic 30.0%
ATAGATACCTTAGACAAGAA+TGG - chr4_4:64416654-64416673 None:intergenic 30.0%
TTAATTGAGTATTCTCCACT+TGG + chr4_4:64416710-64416729 Msa0695010:intron 30.0%
AAATTTATGTCTGCGATTGA+TGG + chr4_4:64416750-64416769 Msa0695010:intron 30.0%
CCAAGACAACAATCATAATA+AGG - chr4_4:64417094-64417113 None:intergenic 30.0%
CCTTATTATGATTGTTGTCT+TGG + chr4_4:64417091-64417110 Msa0695010:intron 30.0%
! TCTACCTGTTTATTGTTTTC+AGG + chr4_4:64417240-64417259 Msa0695010:intron 30.0%
! CTAATCAGTTTGTTTACATC+AGG - chr4_4:64417274-64417293 None:intergenic 30.0%
CTGATGTAAACAAACTGATT+AGG + chr4_4:64417272-64417291 Msa0695010:intron 30.0%
CTAAGAAATGTTGCATTCAA+TGG + chr4_4:64417328-64417347 Msa0695010:intron 30.0%
AAAGCATAAGTTCGTTATTG+TGG - chr4_4:64417472-64417491 None:intergenic 30.0%
! TTATGCTTTGAACTAGATAG+AGG + chr4_4:64417483-64417502 Msa0695010:intron 30.0%
!!! TGCATAAAGTTTTGAAACTG+TGG - chr4_4:64417728-64417747 None:intergenic 30.0%
GAGGAATAGACTAATAGAAA+AGG - chr4_4:64417883-64417902 None:intergenic 30.0%
GCTGTAAAAGAAACAATGAT+AGG - chr4_4:64417961-64417980 None:intergenic 30.0%
! ACCTTTAGAAATGTTTCTCA+AGG + chr4_4:64418078-64418097 Msa0695010:CDS 30.0%
ACCTTGAGAAACATTTCTAA+AGG - chr4_4:64418082-64418101 None:intergenic 30.0%
GAAATATGAATTCCAAGTGA+TGG - chr4_4:64418536-64418555 None:intergenic 30.0%
TCATTAGATATCCAAACACA+AGG - chr4_4:64418565-64418584 None:intergenic 30.0%
GTGTTTGGATATCTAATGAT+CGG + chr4_4:64418566-64418585 Msa0695010:three_prime_UTR 30.0%
GATCGGTAGAATAAATCAAA+TGG + chr4_4:64418583-64418602 Msa0695010:three_prime_UTR 30.0%
AAGATGGTGAAAAATGAACT+TGG + chr4_4:64418700-64418719 Msa0695010:three_prime_UTR 30.0%
ACAAAGATTTCATTCCGACA+AGG + chr4_4:64415658-64415677 Msa0695010:five_prime_UTR 35.0%
! TGCTAGTTTAACTACTTGTC+CGG + chr4_4:64415703-64415722 Msa0695010:five_prime_UTR 35.0%
ATTTGATACTATAGGGTAGG+TGG + chr4_4:64415755-64415774 Msa0695010:five_prime_UTR 35.0%
TTTGATACTATAGGGTAGGT+GGG + chr4_4:64415756-64415775 Msa0695010:five_prime_UTR 35.0%
AAAACATCTTCAGGAAAAGG+TGG - chr4_4:64415974-64415993 None:intergenic 35.0%
! ACCTTTTCCTGAAGATGTTT+TGG + chr4_4:64415973-64415992 Msa0695010:CDS 35.0%
TCCAAAACATCTTCAGGAAA+AGG - chr4_4:64415977-64415996 None:intergenic 35.0%
!! TGGCAAAGTTGTTTCTCAAT+TGG + chr4_4:64416018-64416037 Msa0695010:CDS 35.0%
!! GGCAAAGTTGTTTCTCAATT+GGG + chr4_4:64416019-64416038 Msa0695010:CDS 35.0%
GCACATACACTTCTCATATT+AGG - chr4_4:64416341-64416360 None:intergenic 35.0%
AACCATTTGCCCAATAGTAT+AGG - chr4_4:64416402-64416421 None:intergenic 35.0%
ATACTATTGGGCAAATGGTT+TGG + chr4_4:64416402-64416421 Msa0695010:intron 35.0%
TACTATTGGGCAAATGGTTT+GGG + chr4_4:64416403-64416422 Msa0695010:intron 35.0%
! GGTGTGTGTGTTTTATATGT+AGG + chr4_4:64416501-64416520 Msa0695010:intron 35.0%
TCTTGAACCATTCTTGTCTA+AGG + chr4_4:64416644-64416663 Msa0695010:CDS 35.0%
GTCTGTATAAAGTAATGCAG+TGG + chr4_4:64417139-64417158 Msa0695010:intron 35.0%
GGGTATGTATCTGATTCTTT+AGG - chr4_4:64417191-64417210 None:intergenic 35.0%
! TACATGACTTTTACAGTTGG+TGG - chr4_4:64417410-64417429 None:intergenic 35.0%
! GGATACATGACTTTTACAGT+TGG - chr4_4:64417413-64417432 None:intergenic 35.0%
AAAAGTCATGTATCCATTGC+AGG + chr4_4:64417418-64417437 Msa0695010:intron 35.0%
ACGTTCTTATTAGCAACGAT+TGG + chr4_4:64417650-64417669 Msa0695010:intron 35.0%
AAGTATGACCATTTCAGAGT+CGG + chr4_4:64417783-64417802 Msa0695010:intron 35.0%
ATGTTTACATTCCCTGTTGT+TGG + chr4_4:64417845-64417864 Msa0695010:intron 35.0%
GTGTAAACTATCCAACAACA+GGG - chr4_4:64417859-64417878 None:intergenic 35.0%
TGTGTAAACTATCCAACAAC+AGG - chr4_4:64417860-64417879 None:intergenic 35.0%
! GCTTTAAGCAGGATGAAAAT+CGG + chr4_4:64418176-64418195 Msa0695010:intron 35.0%
CTGCATCAATTGTGTACAAA+CGG + chr4_4:64418317-64418336 Msa0695010:CDS 35.0%
ACCTGTGGAACTTTGAATTT+GGG - chr4_4:64418367-64418386 None:intergenic 35.0%
AACCTGTGGAACTTTGAATT+TGG - chr4_4:64418368-64418387 None:intergenic 35.0%
AGGATAGTTCAATGACTTGA+CGG + chr4_4:64418395-64418414 Msa0695010:CDS 35.0%
TTTGAAGGTCTGATCCTTGT+CGG - chr4_4:64415675-64415694 None:intergenic 40.0%
! TTGGTGACAGATACTGTTCT+TGG - chr4_4:64415863-64415882 None:intergenic 40.0%
AGTATCTGTCACCAAAGTCA+TGG + chr4_4:64415868-64415887 Msa0695010:CDS 40.0%
GGAAGAAGAATCCATGACTT+TGG - chr4_4:64415882-64415901 None:intergenic 40.0%
CTCTGTTCCAAAACATCTTC+AGG - chr4_4:64415983-64416002 None:intergenic 40.0%
! TGAATCATGGTAGCTGATTC+AGG - chr4_4:64416049-64416068 None:intergenic 40.0%
!! AAAGTGATGATGGGGATCTA+AGG + chr4_4:64416122-64416141 Msa0695010:CDS 40.0%
!! AAGTGATGATGGGGATCTAA+GGG + chr4_4:64416123-64416142 Msa0695010:CDS 40.0%
TTTGCCCAATAGTATAGGCT+GGG - chr4_4:64416397-64416416 None:intergenic 40.0%
ATTTGCCCAATAGTATAGGC+TGG - chr4_4:64416398-64416417 None:intergenic 40.0%
AGCCTATACTATTGGGCAAA+TGG + chr4_4:64416397-64416416 Msa0695010:intron 40.0%
ACTATTGGGCAAATGGTTTG+GGG + chr4_4:64416404-64416423 Msa0695010:intron 40.0%
! TGCGATTGATGGTTAGTTCA+TGG + chr4_4:64416761-64416780 Msa0695010:intron 40.0%
TACTTATTCCTCACACAGCA+TGG - chr4_4:64416870-64416889 None:intergenic 40.0%
TAAAGTAATGCAGTGGCCAT+TGG + chr4_4:64417146-64417165 Msa0695010:intron 40.0%
TCCATTGCAGGTCATTAAAC+TGG + chr4_4:64417430-64417449 Msa0695010:intron 40.0%
CCATTGCAGGTCATTAAACT+GGG + chr4_4:64417431-64417450 Msa0695010:intron 40.0%
ACTTAGTGCTCAGCATAAAC+AGG + chr4_4:64417555-64417574 Msa0695010:intron 40.0%
AAATTTATAACCCGTGACCG+CGG - chr4_4:64417810-64417829 None:intergenic 40.0%
AGTCTATTCCTCATCTGCTT+CGG + chr4_4:64417891-64417910 Msa0695010:intron 40.0%
!! CGGATTGAAAGTGTTCAATG+AGG + chr4_4:64417911-64417930 Msa0695010:intron 40.0%
TGATAGGTGAGATGAAGTAC+TGG - chr4_4:64417945-64417964 None:intergenic 40.0%
CTAAGAGTAGTTCCTGTCAT+CGG + chr4_4:64417986-64418005 Msa0695010:CDS 40.0%
!! AATACTGATTCCACTTCTGC+AGG - chr4_4:64418052-64418071 None:intergenic 40.0%
!!! TGACTGCCGGAATTTTGATT+AGG + chr4_4:64418293-64418312 Msa0695010:CDS 40.0%
!!! GACTGCCGGAATTTTGATTA+GGG + chr4_4:64418294-64418313 Msa0695010:CDS 40.0%
TGCAGCCCTAATCAAAATTC+CGG - chr4_4:64418302-64418321 None:intergenic 40.0%
TCCCAAATTCAAAGTTCCAC+AGG + chr4_4:64418363-64418382 Msa0695010:CDS 40.0%
ATCTCCTGAAAGAAAGGAAG+AGG + chr4_4:64418503-64418522 Msa0695010:three_prime_UTR 40.0%
ATTTCTGCTCACCTTGTGTT+TGG + chr4_4:64418551-64418570 Msa0695010:three_prime_UTR 40.0%
!!! TCCAGACTAATTTTGGGATC+TGG + chr4_4:64418625-64418644 Msa0695010:three_prime_UTR 40.0%
TCCAGATCCCAAAATTAGTC+TGG - chr4_4:64418629-64418648 None:intergenic 40.0%
TCTTCTTCCAACGTTACCAC+TGG + chr4_4:64415893-64415912 Msa0695010:CDS 45.0%
ATCTCAGAGACTTCTTGCGT+TGG + chr4_4:64415919-64415938 Msa0695010:CDS 45.0%
ACATCTTCAGGAAAAGGTGG+TGG - chr4_4:64415971-64415990 None:intergenic 45.0%
!! GAACAGAGCATTGAAGAGAG+TGG + chr4_4:64415995-64416014 Msa0695010:CDS 45.0%
AGATAACGTGTGTCTGACAC+AGG + chr4_4:64416309-64416328 Msa0695010:intron 45.0%
GATAACGTGTGTCTGACACA+GGG + chr4_4:64416310-64416329 Msa0695010:intron 45.0%
TGTGTCCCAGCCTATACTAT+TGG + chr4_4:64416389-64416408 Msa0695010:intron 45.0%
GTGTCCCAGCCTATACTATT+GGG + chr4_4:64416390-64416409 Msa0695010:intron 45.0%
AGGGAATGAATTGAGGGTTG+TGG + chr4_4:64416477-64416496 Msa0695010:intron 45.0%
GAATGAATTGAGGGTTGTGG+TGG + chr4_4:64416480-64416499 Msa0695010:intron 45.0%
GAGAGGCAGAAGAGGAAATT+GGG + chr4_4:64416589-64416608 Msa0695010:CDS 45.0%
! TGCTCTGAGTCTGAGATGAT+TGG + chr4_4:64416897-64416916 Msa0695010:intron 45.0%
! GAGTCTGAGATGATTGGAGA+TGG + chr4_4:64416903-64416922 Msa0695010:intron 45.0%
! TCTGAGATGATTGGAGATGG+AGG + chr4_4:64416906-64416925 Msa0695010:intron 45.0%
CTGAGATGATTGGAGATGGA+GGG + chr4_4:64416907-64416926 Msa0695010:intron 45.0%
TCAGCTGCACTTCATGTATC+TGG - chr4_4:64417047-64417066 None:intergenic 45.0%
AGATACATGAAGTGCAGCTG+AGG + chr4_4:64417046-64417065 Msa0695010:intron 45.0%
ATCCCATTCCCATTCACCAA+TGG - chr4_4:64417165-64417184 None:intergenic 45.0%
TTCTTCACTGTCCACGTTCA+TGG + chr4_4:64417296-64417315 Msa0695010:intron 45.0%
AGTACGTACAACCATGAACG+TGG - chr4_4:64417310-64417329 None:intergenic 45.0%
CCCAGTTTAATGACCTGCAA+TGG - chr4_4:64417434-64417453 None:intergenic 45.0%
CTTTCAATCCGAAGCAGATG+AGG - chr4_4:64417902-64417921 None:intergenic 45.0%
TGACAGGAACTACTCTTAGG+AGG - chr4_4:64417985-64418004 None:intergenic 45.0%
CGATGACAGGAACTACTCTT+AGG - chr4_4:64417988-64418007 None:intergenic 45.0%
TCATGAAGTATGCCGATGAC+AGG - chr4_4:64418001-64418020 None:intergenic 45.0%
ACTTCTGCAGGATTCAGAAC+AGG - chr4_4:64418040-64418059 None:intergenic 45.0%
! TGTTCTGAATCCTGCAGAAG+TGG + chr4_4:64418039-64418058 Msa0695010:CDS 45.0%
! CTGAAGCGATTGCTTTAAGC+AGG + chr4_4:64418165-64418184 Msa0695010:intron 45.0%
AATTGTGTACAAACGGCCAC+CGG + chr4_4:64418324-64418343 Msa0695010:CDS 45.0%
GGATTCTGCTCTATGAAAGC+CGG - chr4_4:64418346-64418365 None:intergenic 45.0%
AGTTCCACAGGTTGTAAGCA+AGG + chr4_4:64418375-64418394 Msa0695010:CDS 45.0%
CTATCCTTGCTTACAACCTG+TGG - chr4_4:64418382-64418401 None:intergenic 45.0%
GCATCCTCTTCCTTTCTTTC+AGG - chr4_4:64418510-64418529 None:intergenic 45.0%
GCAAACTGCCATCACATCTA+TGG - chr4_4:64418747-64418766 None:intergenic 45.0%
TCTTCCAACGTTACCACTGG+TGG + chr4_4:64415896-64415915 Msa0695010:CDS 50.0%
! AGATCCACCAGTGGTAACGT+TGG - chr4_4:64415903-64415922 None:intergenic 50.0%
!! AAGTCTCTGAGATCCACCAG+TGG - chr4_4:64415912-64415931 None:intergenic 50.0%
! TCTTCAGGAAAAGGTGGTGG+TGG - chr4_4:64415968-64415987 None:intergenic 50.0%
!! CAGAGCATTGAAGAGAGTGG+TGG + chr4_4:64415998-64416017 Msa0695010:CDS 50.0%
TCCAAGCTCTCTACTCACTC+AGG + chr4_4:64416166-64416185 Msa0695010:CDS 50.0%
ACCTGAGTGAGTAGAGAGCT+TGG - chr4_4:64416170-64416189 None:intergenic 50.0%
! GTAGGTGAAGTGTCATTGCC+TGG + chr4_4:64416519-64416538 Msa0695010:intron 50.0%
! GTGAAGTGTCATTGCCTGGT+GGG + chr4_4:64416523-64416542 Msa0695010:CDS 50.0%
! TTCTGCCTCTCGTTTTGCAG+TGG - chr4_4:64416580-64416599 None:intergenic 50.0%
TGCAAAACGAGAGGCAGAAG+AGG + chr4_4:64416581-64416600 Msa0695010:CDS 50.0%
CGAGAGGCAGAAGAGGAAAT+TGG + chr4_4:64416588-64416607 Msa0695010:CDS 50.0%
GGCAGAAGAGGAAATTGGGT+TGG + chr4_4:64416593-64416612 Msa0695010:CDS 50.0%
AGGAGATTCCATGCTGTGTG+AGG + chr4_4:64416859-64416878 Msa0695010:intron 50.0%
ATGCAGTGGCCATTGGTGAA+TGG + chr4_4:64417153-64417172 Msa0695010:intron 50.0%
TGCAGTGGCCATTGGTGAAT+GGG + chr4_4:64417154-64417173 Msa0695010:intron 50.0%
TGGCCATTGGTGAATGGGAA+TGG + chr4_4:64417159-64417178 Msa0695010:intron 50.0%
GGCCATTGGTGAATGGGAAT+GGG + chr4_4:64417160-64417179 Msa0695010:intron 50.0%
GCAGGATGAAAATCGGAGTC+AGG + chr4_4:64418183-64418202 Msa0695010:intron 50.0%
TCAGGAAGAGAGAGAGTGGA+TGG + chr4_4:64418201-64418220 Msa0695010:CDS 50.0%
CAGGAAGAGAGAGAGTGGAT+GGG + chr4_4:64418202-64418221 Msa0695010:CDS 50.0%
CTCATATGGGGATTGACTGC+CGG + chr4_4:64418280-64418299 Msa0695010:CDS 50.0%
TTCTGCTCTATGAAAGCCGG+TGG - chr4_4:64418343-64418362 None:intergenic 50.0%
AGAGGATGCTGTCCATCACT+TGG + chr4_4:64418521-64418540 Msa0695010:three_prime_UTR 50.0%
! GGTGAAGTGTCATTGCCTGG+TGG + chr4_4:64416522-64416541 Msa0695010:intron 55.0%
GTCATTGCCTGGTGGGAAAG+CGG + chr4_4:64416530-64416549 Msa0695010:CDS 55.0%
GAAAGCGGAGGAAGGGGATA+AGG + chr4_4:64416545-64416564 Msa0695010:CDS 55.0%
!! GAAGTGCAGCTGAGGTACTG+AGG + chr4_4:64417054-64417073 Msa0695010:intron 55.0%
!! ACAGTTGGTGGCACGAGCAA+TGG - chr4_4:64417398-64417417 None:intergenic 55.0%
ACCATTTCAGAGTCGGTCCG+CGG + chr4_4:64417790-64417809 Msa0695010:intron 55.0%
GGAGTCAGGAAGAGAGAGAG+TGG + chr4_4:64418197-64418216 Msa0695010:CDS 55.0%
ATTGCCTGGTGGGAAAGCGG+AGG + chr4_4:64416533-64416552 Msa0695010:CDS 60.0%
CTGGTGGGAAAGCGGAGGAA+GGG + chr4_4:64416538-64416557 Msa0695010:CDS 60.0%
TGGTGGGAAAGCGGAGGAAG+GGG + chr4_4:64416539-64416558 Msa0695010:CDS 60.0%
GGAAGGGGATAAGGACGACG+CGG + chr4_4:64416554-64416573 Msa0695010:CDS 60.0%
! ACCGCGGACCGACTCTGAAA+TGG - chr4_4:64417794-64417813 None:intergenic 60.0%
CCTTCCTCCGCTTTCCCACC+AGG - chr4_4:64416540-64416559 None:intergenic 65.0%
CCTGGTGGGAAAGCGGAGGA+AGG + chr4_4:64416537-64416556 Msa0695010:CDS 65.0%
CGCGGCCACTGCAAAACGAG+AGG + chr4_4:64416572-64416591 Msa0695010:CDS 65.0%
TCAGAGTCGGTCCGCGGTCA+CGG + chr4_4:64417796-64417815 Msa0695010:intron 65.0%
CAGAGTCGGTCCGCGGTCAC+GGG + chr4_4:64417797-64417816 Msa0695010:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 64415635 64418795 64415635 ID=Msa0695010;Name=Msa0695010
chr4_4 mRNA 64415635 64418795 64415635 ID=Msa0695010-mRNA-1;Parent=Msa0695010;Name=Msa0695010-mRNA-1;_AED=0.06;_eAED=0.06;_QI=186|1|1|1|1|1|4|381|285
chr4_4 exon 64415635 64416187 64415635 ID=Msa0695010-mRNA-1:exon:18426;Parent=Msa0695010-mRNA-1
chr4_4 exon 64416523 64416665 64416523 ID=Msa0695010-mRNA-1:exon:18427;Parent=Msa0695010-mRNA-1
chr4_4 exon 64417980 64418099 64417980 ID=Msa0695010-mRNA-1:exon:18428;Parent=Msa0695010-mRNA-1
chr4_4 exon 64418187 64418795 64418187 ID=Msa0695010-mRNA-1:exon:18429;Parent=Msa0695010-mRNA-1
chr4_4 five_prime_UTR 64415635 64415820 64415635 ID=Msa0695010-mRNA-1:five_prime_utr;Parent=Msa0695010-mRNA-1
chr4_4 CDS 64415821 64416187 64415821 ID=Msa0695010-mRNA-1:cds;Parent=Msa0695010-mRNA-1
chr4_4 CDS 64416523 64416665 64416523 ID=Msa0695010-mRNA-1:cds;Parent=Msa0695010-mRNA-1
chr4_4 CDS 64417980 64418099 64417980 ID=Msa0695010-mRNA-1:cds;Parent=Msa0695010-mRNA-1
chr4_4 CDS 64418187 64418414 64418187 ID=Msa0695010-mRNA-1:cds;Parent=Msa0695010-mRNA-1
chr4_4 three_prime_UTR 64418415 64418795 64418415 ID=Msa0695010-mRNA-1:three_prime_utr;Parent=Msa0695010-mRNA-1
Gene Sequence

>Msa0695010

ATGAATTCAATTCTAAGAACAATAACAACAACGTTAATACCAAGAACAGTATCTGTCACCAAAGTCATGGATTCTTCTTCCAACGTTACCACTGGTGGATCTCAGAGACTTCTTGCGTTGGCACAACATCTTCGTCTCTACAAGCCACCACCACCTTTTCCTGAAGATGTTTTGGAACAGAGCATTGAAGAGAGTGGTGGCAAAGTTGTTTCTCAATTGGGTTTTCCTGAATCAGCTACCATGATTCAAAATCCAGATAATTTTAGTCCTAAAAAAGCTGCTGTTTTGATTTGTTTATTTGAAAGTGATGATGGGGATCTAAGGGTTATTCTCACTAAACGATCTTCCAAGCTCTCTACTCACTCAGGTGAAGTGTCATTGCCTGGTGGGAAAGCGGAGGAAGGGGATAAGGACGACGCGGCCACTGCAAAACGAGAGGCAGAAGAGGAAATTGGGTTGGATCCAAAACTAGTCAATGTTGTTACTGTTCTTGAACCATTCTTGTCTAAGCACCTCCTAAGAGTAGTTCCTGTCATCGGCATACTTCATGATAAGAAAGCGTTCAAACCTGTTCTGAATCCTGCAGAAGTGGAATCAGTATTTGACGCACCTTTAGAAATGTTTCTCAAGGATGAAAATCGGAGTCAGGAAGAGAGAGAGTGGATGGGAGAGAAGTATTTGATACATTTTTTTGACTATATTGACATCGAGCAGAAAAAATATCTCATATGGGGATTGACTGCCGGAATTTTGATTAGGGCTGCATCAATTGTGTACAAACGGCCACCGGCTTTCATAGAGCAGAATCCCAAATTCAAAGTTCCACAGGTTGTAAGCAAGGATAGTTCAATGACTTGA
Protein sequence

>Msa0695010

MNSILRTITTTLIPRTVSVTKVMDSSSNVTTGGSQRLLALAQHLRLYKPPPPFPEDVLEQSIEESGGKVVSQLGFPESATMIQNPDNFSPKKAAVLICLFESDDGDLRVILTKRSSKLSTHSGEVSLPGGKAEEGDKDDAATAKREAEEEIGLDPKLVNVVTVLEPFLSKHLLRVVPVIGILHDKKAFKPVLNPAEVESVFDAPLEMFLKDENRSQEEREWMGEKYLIHFFDYIDIEQKKYLIWGLTAGILIRAASIVYKRPPAFIEQNPKFKVPQVVSKDSSMT*