AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0695110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0695110 MtrunA17_Chr4g0046091 90.625 288 24 2 1 287 1 286 0.0 523
Msa0695110 MtrunA17_Chr2g0283341 44.492 236 100 5 29 264 1 205 1.55e-61 195
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0695110 AT1G28960.3 65.157 287 93 5 3 286 10 292 2.42e-120 347
Msa0695110 AT1G28960.1 65.157 287 93 5 3 286 10 292 2.42e-120 347
Msa0695110 AT1G28960.5 65.157 287 93 5 3 286 10 292 2.42e-120 347
Msa0695110 AT1G28960.7 66.071 280 88 5 3 279 10 285 1.87e-118 341
Msa0695110 AT1G28960.6 66.071 280 88 5 3 279 10 285 1.87e-118 341
Msa0695110 AT2G33980.2 63.052 249 83 4 33 279 18 259 4.88e-102 299
Msa0695110 AT2G33980.4 63.052 249 83 4 33 279 18 259 4.88e-102 299
Msa0695110 AT2G33980.1 63.052 249 83 4 33 279 18 259 3.79e-101 298
Msa0695110 AT2G33980.3 63.052 249 83 4 33 279 18 259 7.55e-101 298
Msa0695110 AT5G45940.1 54.688 192 81 3 87 274 28 217 3.99e-67 209
Msa0695110 AT5G45940.2 53.435 131 56 2 87 214 28 156 1.93e-39 135
Msa0695110 AT5G45940.3 54.264 129 54 2 87 212 28 154 3.91e-39 135

Find 99 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCCTCTTCCTTTCTTTC+AGG 0.144264 4_4:+64535718 None:intergenic
AACCTGTGGAACTTTGAATT+TGG 0.148694 4_4:+64535860 None:intergenic
TGAATCATGGTAGCTGATTC+AGG 0.246118 4_4:+64538185 None:intergenic
GTAACAACATTGACTAGTTT+TGG 0.246703 4_4:+64537607 None:intergenic
TCTTGAACCATTCTTGTCTA+AGG 0.274255 4_4:-64537581 Msa0695110:intron
CTCTGTTCCAAAACATCTTC+AGG 0.287071 4_4:+64538251 None:intergenic
ATTTCTGCTCACCTTGTGTT+TGG 0.302067 4_4:-64535674 Msa0695110:three_prime_UTR
TGGCAAAGTTGTTTCTCAAT+TGG 0.304222 4_4:-64538213 Msa0695110:CDS
ACCTGTGGAACTTTGAATTT+GGG 0.326845 4_4:+64535861 None:intergenic
GCAAACTGCCATCACATCTA+TGG 0.329294 4_4:+64535481 None:intergenic
AAAGTGATGATGGGGATCTA+AGG 0.334445 4_4:-64538109 Msa0695110:CDS
GGCAAAGTTGTTTCTCAATT+GGG 0.351294 4_4:-64538212 Msa0695110:CDS
CAATAACAATTTGATACTAT+AGG 0.355004 4_4:-64538484 Msa0695110:five_prime_UTR
TTGGTGACAGATACTGTTCT+TGG 0.364656 4_4:+64538371 None:intergenic
ACTAGCAGTTTAATGTTTGA+AGG 0.371560 4_4:+64538544 None:intergenic
CGAGAGGCAGAAGAGGAAAT+TGG 0.388816 4_4:-64537636 Msa0695110:CDS
TAGAATAAATCAAATGGAAA+AGG 0.394241 4_4:-64535636 Msa0695110:three_prime_UTR
CGATGACAGGAACTACTCTT+AGG 0.397188 4_4:+64536240 None:intergenic
AATAACAATTTGATACTATA+GGG 0.401857 4_4:-64538483 Msa0695110:five_prime_UTR
TCCAGATCCCAAAATTAGTC+TGG 0.402451 4_4:+64535599 None:intergenic
TGCAGCCCTAATCAAAATTC+CGG 0.409951 4_4:+64535926 None:intergenic
TCAGGAAGAGAGAGAGTGGA+TGG 0.414184 4_4:-64536024 Msa0695110:CDS
TGCTAGTTTAACTACTTGTC+CGG 0.424659 4_4:-64538528 Msa0695110:five_prime_UTR
TACAATAATATATATTCGTC+AGG 0.427184 4_4:-64535757 Msa0695110:three_prime_UTR
AGAATAAATCAAATGGAAAA+GGG 0.430085 4_4:-64535635 Msa0695110:three_prime_UTR
TGTTTATTTGAAAGTGATGA+TGG 0.443684 4_4:-64538119 Msa0695110:CDS
TTGCAAAAGCTAATCTAAAC+CGG 0.444277 4_4:+64538509 None:intergenic
GGAAAGCGGAGGAAGGGATA+AGG 0.446569 4_4:-64537687 Msa0695110:CDS
GATCGGTAGAATAAATCAAA+TGG 0.459510 4_4:-64535642 Msa0695110:three_prime_UTR
ACCTGAGTGAGTAGAGAGCT+TGG 0.464566 4_4:+64538064 None:intergenic
AATACTGATTCCACTTCTGC+AGG 0.466410 4_4:+64536176 None:intergenic
ACTTCTGCAGGATTCAGAAC+AGG 0.470641 4_4:+64536188 None:intergenic
CCTTCCTCCGCTTTCCCACC+AGG 0.471747 4_4:+64537694 None:intergenic
GCTTTAAGCAGGATGAAAAT+CGG 0.478548 4_4:-64536049 Msa0695110:intron
TTTGAAGGTCTGATCCTTGT+CGG 0.479051 4_4:+64538559 None:intergenic
TCCAAAACATCTTCAGGAAA+AGG 0.484289 4_4:+64538257 None:intergenic
GGAATAATAAAATGTTGTAA+AGG 0.484974 4_4:-64535579 Msa0695110:three_prime_UTR
ATTTATATCTCCTGAAAGAA+AGG 0.485574 4_4:-64535728 Msa0695110:three_prime_UTR
AGGAAGGGATAAGGATGAAC+GGG 0.486431 4_4:-64537678 Msa0695110:CDS
GGAAGAAGAATCCATGACTT+TGG 0.487067 4_4:+64538352 None:intergenic
GAAATATGAATTCCAAGTGA+TGG 0.489702 4_4:+64535692 None:intergenic
TGTTCTGAATCCTGCAGAAG+TGG 0.496762 4_4:-64536186 Msa0695110:CDS
GCAGGATGAAAATCGGAGTC+AGG 0.500021 4_4:-64536042 Msa0695110:intron
ACCTTGAGAAACATTTCTAA+AGG 0.503154 4_4:+64536146 None:intergenic
TCTTCCAACGTTACCACTGG+TGG 0.507992 4_4:-64538335 Msa0695110:CDS
TCCCAAATTCAAAGTTCCAC+AGG 0.509189 4_4:-64535862 Msa0695110:CDS
CTGCATCAATTGTGTACAAA+CGG 0.513281 4_4:-64535908 Msa0695110:CDS
CTCATATGGGGATTGACTGC+CGG 0.519065 4_4:-64535945 Msa0695110:CDS
CCTGGTGGGAAAGCGGAGGA+AGG 0.522496 4_4:-64537694 Msa0695110:CDS
ATCTCCTGAAAGAAAGGAAG+AGG 0.524063 4_4:-64535722 Msa0695110:three_prime_UTR
TCATGAAGTATGCCGATGAC+AGG 0.534330 4_4:+64536227 None:intergenic
TCTTCTTCCAACGTTACCAC+TGG 0.535311 4_4:-64538338 Msa0695110:CDS
AGAGGATGCTGTCCATCACT+TGG 0.536088 4_4:-64535704 Msa0695110:three_prime_UTR
CAGGAAGAGAGAGAGTGGAT+GGG 0.539082 4_4:-64536023 Msa0695110:CDS
AAGTGATGATGGGGATCTAA+GGG 0.540717 4_4:-64538108 Msa0695110:CDS
TGAAGAAAAGAAATATATTG+AGG 0.545140 4_4:+64535783 None:intergenic
GTGTTTGGATATCTAATGAT+CGG 0.547839 4_4:-64535659 Msa0695110:three_prime_UTR
ACCTTTAGAAATGTTTCTCA+AGG 0.548045 4_4:-64536147 Msa0695110:intron
GGATTCTGCTCTATGAAAGC+CGG 0.548477 4_4:+64535882 None:intergenic
ATCTCAGAGACTTCTTGCGT+TGG 0.549765 4_4:-64538312 Msa0695110:CDS
AGGCAGAAGAGGAAATTGGT+TGG 0.552420 4_4:-64537632 Msa0695110:CDS
ATTTGATACTATAGGGTAGG+TGG 0.554437 4_4:-64538476 Msa0695110:five_prime_UTR
GTTTATTTGAAAGTGATGAT+GGG 0.557864 4_4:-64538118 Msa0695110:CDS
AGTTCCACAGGTTGTAAGCA+AGG 0.560370 4_4:-64535850 Msa0695110:CDS
GCGGCGCGCTGCAAAACGAG+AGG 0.561419 4_4:-64537652 Msa0695110:CDS
CTATCCTTGCTTACAACCTG+TGG 0.562710 4_4:+64535846 None:intergenic
ATAGATACCTTAGACAAGAA+TGG 0.563801 4_4:+64537574 None:intergenic
AATTGTGTACAAACGGCCAC+CGG 0.564192 4_4:-64535901 Msa0695110:CDS
TCTTCAGGAAAAGGTGGTGG+TGG 0.564320 4_4:+64538266 None:intergenic
GTCATTGCCTGGTGGGAAAG+CGG 0.565959 4_4:-64537701 Msa0695110:CDS
CTGGTGGGAAAGCGGAGGAA+GGG 0.566722 4_4:-64537693 Msa0695110:CDS
GAATAAATCAAATGGAAAAG+GGG 0.577318 4_4:-64535634 Msa0695110:three_prime_UTR
TGAAATGATAAGCAATAAGA+TGG 0.579545 4_4:-64535541 Msa0695110:three_prime_UTR
AGTATCTGTCACCAAAGTCA+TGG 0.580073 4_4:-64538363 Msa0695110:CDS
CTAAGAGTAGTTCCTGTCAT+CGG 0.582497 4_4:-64536239 Msa0695110:CDS
TGCAAAACGAGAGGCAGAAG+AGG 0.583690 4_4:-64537643 Msa0695110:CDS
GGAGTCAGGAAGAGAGAGAG+TGG 0.584588 4_4:-64536028 Msa0695110:CDS
GTAGGTGAAGTGTCATTGCC+TGG 0.586937 4_4:-64537712 Msa0695110:intron
AGGATAGTTCAATGACTTGA+CGG 0.587268 4_4:-64535830 Msa0695110:exon
ACATCTTCAGGAAAAGGTGG+TGG 0.591697 4_4:+64538263 None:intergenic
TCCAAGCTCTCTACTCACTC+AGG 0.594205 4_4:-64538065 Msa0695110:intron
ACAATTTGATACTATAGGGT+AGG 0.599534 4_4:-64538479 Msa0695110:five_prime_UTR
TTTGATACTATAGGGTAGGT+GGG 0.601183 4_4:-64538475 Msa0695110:five_prime_UTR
GATAAGGATGAACGGGACGG+CGG 0.605481 4_4:-64537671 Msa0695110:CDS
TGACAGGAACTACTCTTAGG+AGG 0.607814 4_4:+64536243 None:intergenic
TTTATTTGAAAGTGATGATG+GGG 0.614807 4_4:-64538117 Msa0695110:CDS
GAGGAAGGGATAAGGATGAA+CGG 0.617258 4_4:-64537679 Msa0695110:CDS
AGGGATAAGGATGAACGGGA+CGG 0.621181 4_4:-64537674 Msa0695110:CDS
AAAACATCTTCAGGAAAAGG+TGG 0.635918 4_4:+64538260 None:intergenic
GAACAGAGCATTGAAGAGAG+TGG 0.639203 4_4:-64538236 Msa0695110:CDS
TTCTGCTCTATGAAAGCCGG+TGG 0.643205 4_4:+64535885 None:intergenic
AGATCCACCAGTGGTAACGT+TGG 0.652080 4_4:+64538331 None:intergenic
GTGAAGTGTCATTGCCTGGT+GGG 0.658192 4_4:-64537708 Msa0695110:CDS
ATTGCCTGGTGGGAAAGCGG+AGG 0.658525 4_4:-64537698 Msa0695110:CDS
ACAAAGATTTCATTCCGACA+AGG 0.669608 4_4:-64538573 Msa0695110:five_prime_UTR
CAGAGCATTGAAGAGAGTGG+TGG 0.680698 4_4:-64538233 Msa0695110:CDS
GGTGAAGTGTCATTGCCTGG+TGG 0.686704 4_4:-64537709 Msa0695110:CDS
TCATTAGATATCCAAACACA+AGG 0.698944 4_4:+64535663 None:intergenic
AAGTCTCTGAGATCCACCAG+TGG 0.760789 4_4:+64538322 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATCATTTTAAGAAAA+GGG + chr4_4:64537034-64537053 None:intergenic 10.0%
!!! TTATAAATCATTTTAAGAAA+AGG + chr4_4:64537035-64537054 None:intergenic 10.0%
!! AATAACAATTTGATACTATA+GGG - chr4_4:64535565-64535584 Msa0695110:three_prime_UTR 15.0%
!!! TTTTCTTTAATAGGAAAAAA+AGG + chr4_4:64537187-64537206 None:intergenic 15.0%
!!! AATCAATTTGAGAAATTTTT+CGG - chr4_4:64537503-64537522 Msa0695110:intron 15.0%
!!! TCTTTGTCTTAGATTTTATA+TGG + chr4_4:64535462-64535481 None:intergenic 20.0%
!! CAATAACAATTTGATACTAT+AGG - chr4_4:64535564-64535583 Msa0695110:three_prime_UTR 20.0%
!!! TTTTTAGGACTAAAATTATC+TGG + chr4_4:64535893-64535912 None:intergenic 20.0%
!!! TTTTTTTAGACAAATGTTAG+TGG - chr4_4:64536086-64536105 Msa0695110:intron 20.0%
!!! TACTTTAGTTATTGTGATTT+TGG - chr4_4:64536257-64536276 Msa0695110:intron 20.0%
!!! TTTTGGTTATTTGAATTTAG+AGG - chr4_4:64536274-64536293 Msa0695110:intron 20.0%
!! TTTGGTTATTTGAATTTAGA+GGG - chr4_4:64536275-64536294 Msa0695110:intron 20.0%
!!! TTTCAGCAATTTTCTTTAAT+AGG + chr4_4:64537196-64537215 None:intergenic 20.0%
!!! TTTTTCGGAAATTTATTTGT+TGG - chr4_4:64537518-64537537 Msa0695110:intron 20.0%
!!! GAAATTTATTTGTTGGATAA+TGG - chr4_4:64537525-64537544 Msa0695110:intron 20.0%
!! GGGTTATAAATTTGTATTAT+TGG - chr4_4:64537640-64537659 Msa0695110:CDS 20.0%
!! TGAAGAAAAGAAATATATTG+AGG + chr4_4:64538268-64538287 None:intergenic 20.0%
!! TACAATAATATATATTCGTC+AGG - chr4_4:64538291-64538310 Msa0695110:CDS 20.0%
!! TAGAATAAATCAAATGGAAA+AGG - chr4_4:64538412-64538431 Msa0695110:CDS 20.0%
!! AGAATAAATCAAATGGAAAA+GGG - chr4_4:64538413-64538432 Msa0695110:CDS 20.0%
!!! AAAAAATTCCAGACTAATTT+TGG - chr4_4:64538441-64538460 Msa0695110:five_prime_UTR 20.0%
!!! AAAAATTCCAGACTAATTTT+GGG - chr4_4:64538442-64538461 Msa0695110:five_prime_UTR 20.0%
!! GGAATAATAAAATGTTGTAA+AGG - chr4_4:64538469-64538488 Msa0695110:five_prime_UTR 20.0%
!! ATTATCTGGATTTTGAATCA+TGG + chr4_4:64535879-64535898 None:intergenic 25.0%
! TGTTTATTTGAAAGTGATGA+TGG - chr4_4:64535929-64535948 Msa0695110:CDS 25.0%
! GTTTATTTGAAAGTGATGAT+GGG - chr4_4:64535930-64535949 Msa0695110:CDS 25.0%
!! TTTATTTGAAAGTGATGATG+GGG - chr4_4:64535931-64535950 Msa0695110:CDS 25.0%
! TAAAGATTCAAAATTCCAAG+TGG + chr4_4:64536551-64536570 None:intergenic 25.0%
!!! CTAGAAAATGTTGATTTTGT+TGG - chr4_4:64536619-64536638 Msa0695110:intron 25.0%
!! TGTAATTATTTAGCTCATGA+AGG - chr4_4:64536662-64536681 Msa0695110:intron 25.0%
! ACTTCATTCTTGAATATCAT+CGG - chr4_4:64536804-64536823 Msa0695110:intron 25.0%
! AATTCCTGAAAACAATAAAC+AGG + chr4_4:64537070-64537089 None:intergenic 25.0%
! TAACAACACTTCATAGATTT+AGG - chr4_4:64537418-64537437 Msa0695110:intron 25.0%
! GATACAAATAAAACTAAGCA+TGG + chr4_4:64537929-64537948 None:intergenic 25.0%
! AGCAGAAAAAATATCTCATA+TGG - chr4_4:64538089-64538108 Msa0695110:CDS 25.0%
! GCAGAAAAAATATCTCATAT+GGG - chr4_4:64538090-64538109 Msa0695110:CDS 25.0%
! CAGAAAAAATATCTCATATG+GGG - chr4_4:64538091-64538110 Msa0695110:CDS 25.0%
! ATTTATATCTCCTGAAAGAA+AGG - chr4_4:64538320-64538339 Msa0695110:CDS 25.0%
! GAATAAATCAAATGGAAAAG+GGG - chr4_4:64538414-64538433 Msa0695110:exon 25.0%
! TGAAATGATAAGCAATAAGA+TGG - chr4_4:64538507-64538526 Msa0695110:five_prime_UTR 25.0%
!! TTTTTAATCCATAGATGTGA+TGG - chr4_4:64538559-64538578 Msa0695110:five_prime_UTR 25.0%
ACTAGCAGTTTAATGTTTGA+AGG + chr4_4:64535507-64535526 None:intergenic 30.0%
TTGCAAAAGCTAATCTAAAC+CGG + chr4_4:64535542-64535561 None:intergenic 30.0%
ACAATTTGATACTATAGGGT+AGG - chr4_4:64535569-64535588 Msa0695110:three_prime_UTR 30.0%
!!! ATCAAAACAGCAGCTTTTTT+AGG + chr4_4:64535908-64535927 None:intergenic 30.0%
AATTTAGAGGGAATGAATTG+AGG - chr4_4:64536287-64536306 Msa0695110:intron 30.0%
ATTTAGAGGGAATGAATTGA+GGG - chr4_4:64536288-64536307 Msa0695110:intron 30.0%
!!! GTAACAACATTGACTAGTTT+TGG + chr4_4:64536444-64536463 None:intergenic 30.0%
ATAGATACCTTAGACAAGAA+TGG + chr4_4:64536477-64536496 None:intergenic 30.0%
TTAATTGAGTATTCTCCACT+TGG - chr4_4:64536533-64536552 Msa0695110:intron 30.0%
AAATTTATGTCTGCGATTGA+TGG - chr4_4:64536573-64536592 Msa0695110:intron 30.0%
CCAAGACAACAATCATAATA+AGG + chr4_4:64536917-64536936 None:intergenic 30.0%
CCTTATTATGATTGTTGTCT+TGG - chr4_4:64536914-64536933 Msa0695110:intron 30.0%
! TCTACCTGTTTATTGTTTTC+AGG - chr4_4:64537063-64537082 Msa0695110:intron 30.0%
! CTAATCAGTTTGTTTACATC+AGG + chr4_4:64537097-64537116 None:intergenic 30.0%
CTGATGTAAACAAACTGATT+AGG - chr4_4:64537095-64537114 Msa0695110:intron 30.0%
CTAAGAAATGTTGCATTCAA+TGG - chr4_4:64537151-64537170 Msa0695110:intron 30.0%
AAAGCATAAGTTCGTTATTG+TGG + chr4_4:64537295-64537314 None:intergenic 30.0%
! TTATGCTTTGAACTAGATAG+AGG - chr4_4:64537306-64537325 Msa0695110:intron 30.0%
!!! TGCATAAAGTTTTGAAACTG+TGG + chr4_4:64537551-64537570 None:intergenic 30.0%
GAGGAATAGACTAATAGAAA+AGG + chr4_4:64537706-64537725 None:intergenic 30.0%
GCTGTAAAAGAAACAATGAT+AGG + chr4_4:64537784-64537803 None:intergenic 30.0%
! ACCTTTAGAAATGTTTCTCA+AGG - chr4_4:64537901-64537920 Msa0695110:intron 30.0%
ACCTTGAGAAACATTTCTAA+AGG + chr4_4:64537905-64537924 None:intergenic 30.0%
GAAATATGAATTCCAAGTGA+TGG + chr4_4:64538359-64538378 None:intergenic 30.0%
TCATTAGATATCCAAACACA+AGG + chr4_4:64538388-64538407 None:intergenic 30.0%
GTGTTTGGATATCTAATGAT+CGG - chr4_4:64538389-64538408 Msa0695110:CDS 30.0%
GATCGGTAGAATAAATCAAA+TGG - chr4_4:64538406-64538425 Msa0695110:CDS 30.0%
AAGATGGTGAAAAATGAACT+TGG - chr4_4:64538523-64538542 Msa0695110:five_prime_UTR 30.0%
ACAAAGATTTCATTCCGACA+AGG - chr4_4:64535475-64535494 Msa0695110:three_prime_UTR 35.0%
! TGCTAGTTTAACTACTTGTC+CGG - chr4_4:64535520-64535539 Msa0695110:three_prime_UTR 35.0%
ATTTGATACTATAGGGTAGG+TGG - chr4_4:64535572-64535591 Msa0695110:three_prime_UTR 35.0%
TTTGATACTATAGGGTAGGT+GGG - chr4_4:64535573-64535592 Msa0695110:three_prime_UTR 35.0%
AAAACATCTTCAGGAAAAGG+TGG + chr4_4:64535791-64535810 None:intergenic 35.0%
! ACCTTTTCCTGAAGATGTTT+TGG - chr4_4:64535790-64535809 Msa0695110:three_prime_UTR 35.0%
TCCAAAACATCTTCAGGAAA+AGG + chr4_4:64535794-64535813 None:intergenic 35.0%
!! TGGCAAAGTTGTTTCTCAAT+TGG - chr4_4:64535835-64535854 Msa0695110:CDS 35.0%
!! GGCAAAGTTGTTTCTCAATT+GGG - chr4_4:64535836-64535855 Msa0695110:CDS 35.0%
GCACATACACTTCTCATATT+AGG + chr4_4:64536158-64536177 None:intergenic 35.0%
AACCATTTGCCCAATAGTAT+AGG + chr4_4:64536219-64536238 None:intergenic 35.0%
ATACTATTGGGCAAATGGTT+TGG - chr4_4:64536219-64536238 Msa0695110:CDS 35.0%
TACTATTGGGCAAATGGTTT+GGG - chr4_4:64536220-64536239 Msa0695110:CDS 35.0%
GTGTGTGTGTGTTTATATGT+AGG - chr4_4:64536318-64536337 Msa0695110:intron 35.0%
TCTTGAACCATTCTTGTCTA+AGG - chr4_4:64536467-64536486 Msa0695110:intron 35.0%
GTCTGTATAAAGTAATGCAG+TGG - chr4_4:64536962-64536981 Msa0695110:intron 35.0%
GGGTATGTATCTGATTCTTT+AGG + chr4_4:64537014-64537033 None:intergenic 35.0%
! TACATGACTTTTACAGTTGG+TGG + chr4_4:64537233-64537252 None:intergenic 35.0%
! GGATACATGACTTTTACAGT+TGG + chr4_4:64537236-64537255 None:intergenic 35.0%
AAAAGTCATGTATCCATTGC+AGG - chr4_4:64537241-64537260 Msa0695110:intron 35.0%
ACGTTCTTATTAGCAACGAT+TGG - chr4_4:64537473-64537492 Msa0695110:intron 35.0%
AAGTATGACCATTTCAGAGT+CGG - chr4_4:64537606-64537625 Msa0695110:CDS 35.0%
ATGTTTACATTCCCTGTTGT+TGG - chr4_4:64537668-64537687 Msa0695110:CDS 35.0%
GTGTAAACTATCCAACAACA+GGG + chr4_4:64537682-64537701 None:intergenic 35.0%
TGTGTAAACTATCCAACAAC+AGG + chr4_4:64537683-64537702 None:intergenic 35.0%
! GCTTTAAGCAGGATGAAAAT+CGG - chr4_4:64537999-64538018 Msa0695110:intron 35.0%
CTGCATCAATTGTGTACAAA+CGG - chr4_4:64538140-64538159 Msa0695110:CDS 35.0%
ACCTGTGGAACTTTGAATTT+GGG + chr4_4:64538190-64538209 None:intergenic 35.0%
AACCTGTGGAACTTTGAATT+TGG + chr4_4:64538191-64538210 None:intergenic 35.0%
AGGATAGTTCAATGACTTGA+CGG - chr4_4:64538218-64538237 Msa0695110:CDS 35.0%
TTTGAAGGTCTGATCCTTGT+CGG + chr4_4:64535492-64535511 None:intergenic 40.0%
! TTGGTGACAGATACTGTTCT+TGG + chr4_4:64535680-64535699 None:intergenic 40.0%
AGTATCTGTCACCAAAGTCA+TGG - chr4_4:64535685-64535704 Msa0695110:three_prime_UTR 40.0%
GGAAGAAGAATCCATGACTT+TGG + chr4_4:64535699-64535718 None:intergenic 40.0%
CTCTGTTCCAAAACATCTTC+AGG + chr4_4:64535800-64535819 None:intergenic 40.0%
! TGAATCATGGTAGCTGATTC+AGG + chr4_4:64535866-64535885 None:intergenic 40.0%
!! AAAGTGATGATGGGGATCTA+AGG - chr4_4:64535939-64535958 Msa0695110:CDS 40.0%
!! AAGTGATGATGGGGATCTAA+GGG - chr4_4:64535940-64535959 Msa0695110:CDS 40.0%
TTTGCCCAATAGTATAGGCT+GGG + chr4_4:64536214-64536233 None:intergenic 40.0%
ATTTGCCCAATAGTATAGGC+TGG + chr4_4:64536215-64536234 None:intergenic 40.0%
AGCCTATACTATTGGGCAAA+TGG - chr4_4:64536214-64536233 Msa0695110:CDS 40.0%
ACTATTGGGCAAATGGTTTG+GGG - chr4_4:64536221-64536240 Msa0695110:CDS 40.0%
! TGCGATTGATGGTTAGTTCA+TGG - chr4_4:64536584-64536603 Msa0695110:intron 40.0%
TACTTATTCCTCACACAGCA+TGG + chr4_4:64536693-64536712 None:intergenic 40.0%
TAAAGTAATGCAGTGGCCAT+TGG - chr4_4:64536969-64536988 Msa0695110:intron 40.0%
TCCATTGCAGGTCATTAAAC+TGG - chr4_4:64537253-64537272 Msa0695110:intron 40.0%
CCATTGCAGGTCATTAAACT+GGG - chr4_4:64537254-64537273 Msa0695110:intron 40.0%
ACTTAGTGCTCAGCATAAAC+AGG - chr4_4:64537378-64537397 Msa0695110:intron 40.0%
AAATTTATAACCCGTGACCG+CGG + chr4_4:64537633-64537652 None:intergenic 40.0%
AGTCTATTCCTCATCTGCTT+CGG - chr4_4:64537714-64537733 Msa0695110:intron 40.0%
!! CGGATTGAAAGTGTTCAATG+AGG - chr4_4:64537734-64537753 Msa0695110:intron 40.0%
TGATAGGTGAGATGAAGTAC+TGG + chr4_4:64537768-64537787 None:intergenic 40.0%
CTAAGAGTAGTTCCTGTCAT+CGG - chr4_4:64537809-64537828 Msa0695110:intron 40.0%
!! AATACTGATTCCACTTCTGC+AGG + chr4_4:64537875-64537894 None:intergenic 40.0%
!!! TGACTGCCGGAATTTTGATT+AGG - chr4_4:64538116-64538135 Msa0695110:CDS 40.0%
!!! GACTGCCGGAATTTTGATTA+GGG - chr4_4:64538117-64538136 Msa0695110:CDS 40.0%
TGCAGCCCTAATCAAAATTC+CGG + chr4_4:64538125-64538144 None:intergenic 40.0%
TCCCAAATTCAAAGTTCCAC+AGG - chr4_4:64538186-64538205 Msa0695110:CDS 40.0%
ATCTCCTGAAAGAAAGGAAG+AGG - chr4_4:64538326-64538345 Msa0695110:CDS 40.0%
ATTTCTGCTCACCTTGTGTT+TGG - chr4_4:64538374-64538393 Msa0695110:CDS 40.0%
!!! TCCAGACTAATTTTGGGATC+TGG - chr4_4:64538448-64538467 Msa0695110:five_prime_UTR 40.0%
TCCAGATCCCAAAATTAGTC+TGG + chr4_4:64538452-64538471 None:intergenic 40.0%
TCTTCTTCCAACGTTACCAC+TGG - chr4_4:64535710-64535729 Msa0695110:three_prime_UTR 45.0%
ATCTCAGAGACTTCTTGCGT+TGG - chr4_4:64535736-64535755 Msa0695110:three_prime_UTR 45.0%
ACATCTTCAGGAAAAGGTGG+TGG + chr4_4:64535788-64535807 None:intergenic 45.0%
!! GAACAGAGCATTGAAGAGAG+TGG - chr4_4:64535812-64535831 Msa0695110:three_prime_UTR 45.0%
AGATAACGTGTGTCTGACAC+AGG - chr4_4:64536126-64536145 Msa0695110:intron 45.0%
GATAACGTGTGTCTGACACA+GGG - chr4_4:64536127-64536146 Msa0695110:intron 45.0%
TGTGTCCCAGCCTATACTAT+TGG - chr4_4:64536206-64536225 Msa0695110:CDS 45.0%
GTGTCCCAGCCTATACTATT+GGG - chr4_4:64536207-64536226 Msa0695110:CDS 45.0%
AGGGAATGAATTGAGGGTTG+TGG - chr4_4:64536294-64536313 Msa0695110:intron 45.0%
GAGGAAGGGATAAGGATGAA+CGG - chr4_4:64536369-64536388 Msa0695110:intron 45.0%
AGGAAGGGATAAGGATGAAC+GGG - chr4_4:64536370-64536389 Msa0695110:intron 45.0%
AGGCAGAAGAGGAAATTGGT+TGG - chr4_4:64536416-64536435 Msa0695110:intron 45.0%
! TGCTCTGAGTCTGAGATGAT+TGG - chr4_4:64536720-64536739 Msa0695110:intron 45.0%
! GAGTCTGAGATGATTGGAGA+TGG - chr4_4:64536726-64536745 Msa0695110:intron 45.0%
! TCTGAGATGATTGGAGATGG+AGG - chr4_4:64536729-64536748 Msa0695110:intron 45.0%
CTGAGATGATTGGAGATGGA+GGG - chr4_4:64536730-64536749 Msa0695110:intron 45.0%
TCAGCTGCACTTCATGTATC+TGG + chr4_4:64536870-64536889 None:intergenic 45.0%
AGATACATGAAGTGCAGCTG+AGG - chr4_4:64536869-64536888 Msa0695110:intron 45.0%
ATCCCATTCCCATTCACCAA+TGG + chr4_4:64536988-64537007 None:intergenic 45.0%
TTCTTCACTGTCCACGTTCA+TGG - chr4_4:64537119-64537138 Msa0695110:intron 45.0%
AGTACGTACAACCATGAACG+TGG + chr4_4:64537133-64537152 None:intergenic 45.0%
CCCAGTTTAATGACCTGCAA+TGG + chr4_4:64537257-64537276 None:intergenic 45.0%
CTTTCAATCCGAAGCAGATG+AGG + chr4_4:64537725-64537744 None:intergenic 45.0%
TGACAGGAACTACTCTTAGG+AGG + chr4_4:64537808-64537827 None:intergenic 45.0%
CGATGACAGGAACTACTCTT+AGG + chr4_4:64537811-64537830 None:intergenic 45.0%
TCATGAAGTATGCCGATGAC+AGG + chr4_4:64537824-64537843 None:intergenic 45.0%
ACTTCTGCAGGATTCAGAAC+AGG + chr4_4:64537863-64537882 None:intergenic 45.0%
! TGTTCTGAATCCTGCAGAAG+TGG - chr4_4:64537862-64537881 Msa0695110:intron 45.0%
! CTGAAGCGATTGCTTTAAGC+AGG - chr4_4:64537988-64538007 Msa0695110:intron 45.0%
AATTGTGTACAAACGGCCAC+CGG - chr4_4:64538147-64538166 Msa0695110:CDS 45.0%
GGATTCTGCTCTATGAAAGC+CGG + chr4_4:64538169-64538188 None:intergenic 45.0%
AGTTCCACAGGTTGTAAGCA+AGG - chr4_4:64538198-64538217 Msa0695110:CDS 45.0%
CTATCCTTGCTTACAACCTG+TGG + chr4_4:64538205-64538224 None:intergenic 45.0%
GCATCCTCTTCCTTTCTTTC+AGG + chr4_4:64538333-64538352 None:intergenic 45.0%
GCAAACTGCCATCACATCTA+TGG + chr4_4:64538570-64538589 None:intergenic 45.0%
TCTTCCAACGTTACCACTGG+TGG - chr4_4:64535713-64535732 Msa0695110:three_prime_UTR 50.0%
! AGATCCACCAGTGGTAACGT+TGG + chr4_4:64535720-64535739 None:intergenic 50.0%
!! AAGTCTCTGAGATCCACCAG+TGG + chr4_4:64535729-64535748 None:intergenic 50.0%
! TCTTCAGGAAAAGGTGGTGG+TGG + chr4_4:64535785-64535804 None:intergenic 50.0%
!! CAGAGCATTGAAGAGAGTGG+TGG - chr4_4:64535815-64535834 Msa0695110:exon 50.0%
TCCAAGCTCTCTACTCACTC+AGG - chr4_4:64535983-64536002 Msa0695110:CDS 50.0%
ACCTGAGTGAGTAGAGAGCT+TGG + chr4_4:64535987-64536006 None:intergenic 50.0%
! GTAGGTGAAGTGTCATTGCC+TGG - chr4_4:64536336-64536355 Msa0695110:intron 50.0%
! GTGAAGTGTCATTGCCTGGT+GGG - chr4_4:64536340-64536359 Msa0695110:intron 50.0%
AGGGATAAGGATGAACGGGA+CGG - chr4_4:64536374-64536393 Msa0695110:intron 50.0%
TGCAAAACGAGAGGCAGAAG+AGG - chr4_4:64536405-64536424 Msa0695110:intron 50.0%
CGAGAGGCAGAAGAGGAAAT+TGG - chr4_4:64536412-64536431 Msa0695110:intron 50.0%
AGGAGATTCCATGCTGTGTG+AGG - chr4_4:64536682-64536701 Msa0695110:intron 50.0%
ATGCAGTGGCCATTGGTGAA+TGG - chr4_4:64536976-64536995 Msa0695110:intron 50.0%
TGCAGTGGCCATTGGTGAAT+GGG - chr4_4:64536977-64536996 Msa0695110:intron 50.0%
TGGCCATTGGTGAATGGGAA+TGG - chr4_4:64536982-64537001 Msa0695110:intron 50.0%
GGCCATTGGTGAATGGGAAT+GGG - chr4_4:64536983-64537002 Msa0695110:intron 50.0%
GCAGGATGAAAATCGGAGTC+AGG - chr4_4:64538006-64538025 Msa0695110:intron 50.0%
TCAGGAAGAGAGAGAGTGGA+TGG - chr4_4:64538024-64538043 Msa0695110:intron 50.0%
CAGGAAGAGAGAGAGTGGAT+GGG - chr4_4:64538025-64538044 Msa0695110:intron 50.0%
CTCATATGGGGATTGACTGC+CGG - chr4_4:64538103-64538122 Msa0695110:CDS 50.0%
TTCTGCTCTATGAAAGCCGG+TGG + chr4_4:64538166-64538185 None:intergenic 50.0%
AGAGGATGCTGTCCATCACT+TGG - chr4_4:64538344-64538363 Msa0695110:CDS 50.0%
! GGTGAAGTGTCATTGCCTGG+TGG - chr4_4:64536339-64536358 Msa0695110:intron 55.0%
GTCATTGCCTGGTGGGAAAG+CGG - chr4_4:64536347-64536366 Msa0695110:intron 55.0%
GGAAAGCGGAGGAAGGGATA+AGG - chr4_4:64536361-64536380 Msa0695110:intron 55.0%
GATAAGGATGAACGGGACGG+CGG - chr4_4:64536377-64536396 Msa0695110:intron 55.0%
!! GAAGTGCAGCTGAGGTACTG+AGG - chr4_4:64536877-64536896 Msa0695110:intron 55.0%
!! ACAGTTGGTGGCACGAGCAA+TGG + chr4_4:64537221-64537240 None:intergenic 55.0%
ACCATTTCAGAGTCGGTCCG+CGG - chr4_4:64537613-64537632 Msa0695110:CDS 55.0%
GGAGTCAGGAAGAGAGAGAG+TGG - chr4_4:64538020-64538039 Msa0695110:intron 55.0%
ATTGCCTGGTGGGAAAGCGG+AGG - chr4_4:64536350-64536369 Msa0695110:intron 60.0%
CTGGTGGGAAAGCGGAGGAA+GGG - chr4_4:64536355-64536374 Msa0695110:intron 60.0%
! ACCGCGGACCGACTCTGAAA+TGG + chr4_4:64537617-64537636 None:intergenic 60.0%
CCTTCCTCCGCTTTCCCACC+AGG + chr4_4:64536357-64536376 None:intergenic 65.0%
CCTGGTGGGAAAGCGGAGGA+AGG - chr4_4:64536354-64536373 Msa0695110:intron 65.0%
TCAGAGTCGGTCCGCGGTCA+CGG - chr4_4:64537619-64537638 Msa0695110:CDS 65.0%
GCGGCGCGCTGCAAAACGAG+AGG - chr4_4:64536396-64536415 Msa0695110:intron 70.0%
CAGAGTCGGTCCGCGGTCAC+GGG - chr4_4:64537620-64537639 Msa0695110:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 64535452 64538618 64535452 ID=Msa0695110;Name=Msa0695110
chr4_4 mRNA 64535452 64538618 64535452 ID=Msa0695110-mRNA-1;Parent=Msa0695110;Name=Msa0695110-mRNA-1;_AED=0.06;_eAED=0.06;_QI=186|1|1|1|1|1|4|381|287
chr4_4 exon 64535452 64536060 64535452 ID=Msa0695110-mRNA-1:exon:18503;Parent=Msa0695110-mRNA-1
chr4_4 exon 64536148 64536267 64536148 ID=Msa0695110-mRNA-1:exon:18502;Parent=Msa0695110-mRNA-1
chr4_4 exon 64537582 64537730 64537582 ID=Msa0695110-mRNA-1:exon:18501;Parent=Msa0695110-mRNA-1
chr4_4 exon 64538066 64538618 64538066 ID=Msa0695110-mRNA-1:exon:18500;Parent=Msa0695110-mRNA-1
chr4_4 five_prime_UTR 64538433 64538618 64538433 ID=Msa0695110-mRNA-1:five_prime_utr;Parent=Msa0695110-mRNA-1
chr4_4 CDS 64538066 64538432 64538066 ID=Msa0695110-mRNA-1:cds;Parent=Msa0695110-mRNA-1
chr4_4 CDS 64537582 64537730 64537582 ID=Msa0695110-mRNA-1:cds;Parent=Msa0695110-mRNA-1
chr4_4 CDS 64536148 64536267 64536148 ID=Msa0695110-mRNA-1:cds;Parent=Msa0695110-mRNA-1
chr4_4 CDS 64535833 64536060 64535833 ID=Msa0695110-mRNA-1:cds;Parent=Msa0695110-mRNA-1
chr4_4 three_prime_UTR 64535452 64535832 64535452 ID=Msa0695110-mRNA-1:three_prime_utr;Parent=Msa0695110-mRNA-1
Gene Sequence

>Msa0695110

ATGAATTCAATTCTAAGAACAATAACAACAACGTTAATACCAAGAACAGTATCTGTCACCAAAGTCATGGATTCTTCTTCCAACGTTACCACTGGTGGATCTCAGAGACTTCTTGCGTTGGCACAACATCTTCGTCTCTACAAGCCACCACCACCTTTTCCTGAAGATGTTTTGGAACAGAGCATTGAAGAGAGTGGTGGCAAAGTTGTTTCTCAATTGGGTTTTCCTGAATCAGCTACCATGATTCAAAATCCAGATAATTTTAGTCCTAAAAAAGCTGCTGTTTTGATTTGTTTATTTGAAAGTGATGATGGGGATCTAAGGGTTATTCTCACTAAACGATCTTCCAAGCTCTCTACTCACTCAGGTGAAGTGTCATTGCCTGGTGGGAAAGCGGAGGAAGGGATAAGGATGAACGGGACGGCGGCGCGCTGCAAAACGAGAGGCAGAAGAGGAAATTGGTTGGATCCAAAACTAGTCAATGTTGTTACTGTTCTTGAACCATTCTTGTCTAAGCACCTCCTAAGAGTAGTTCCTGTCATCGGCATACTTCATGATAAGAAAGCGTTCAAACCTGTTCTGAATCCTGCAGAAGTGGAATCAGTATTTGACGCACCTTTAGAAATGTTTCTCAAGGATGAAAATCGGAGTCAGGAAGAGAGAGAGTGGATGGGAGAGAAGTATTTGATACATTTTTTTGACTATATTGACATCGAGCAGAAAAAATATCTCATATGGGGATTGACTGCCGGAATTTTGATTAGGGCTGCATCAATTGTGTACAAACGGCCACCGGCTTTCATAGAGCAGAATCCCAAATTCAAAGTTCCACAGGTTGTAAGCAAGGATAGTTCAATGACTTGA
Protein sequence

>Msa0695110

MNSILRTITTTLIPRTVSVTKVMDSSSNVTTGGSQRLLALAQHLRLYKPPPPFPEDVLEQSIEESGGKVVSQLGFPESATMIQNPDNFSPKKAAVLICLFESDDGDLRVILTKRSSKLSTHSGEVSLPGGKAEEGIRMNGTAARCKTRGRRGNWLDPKLVNVVTVLEPFLSKHLLRVVPVIGILHDKKAFKPVLNPAEVESVFDAPLEMFLKDENRSQEEREWMGEKYLIHFFDYIDIEQKKYLIWGLTAGILIRAASIVYKRPPAFIEQNPKFKVPQVVSKDSSMT*