Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449260 | G7ITZ6 | 95.556 | 135 | 6 | 0 | 1 | 135 | 1 | 135 | 3.58e-86 | 256 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1449260 | Msa1454900 | 0.964869 | 7.139839e-124 | -8.615850e-47 |
| Msa0202800 | Msa1449260 | 0.804028 | 2.628836e-49 | -8.615850e-47 |
| Msa1244500 | Msa1449260 | 0.800401 | 1.466371e-48 | -8.615850e-47 |
| Msa1285550 | Msa1449260 | 0.820908 | 5.349314e-53 | -8.615850e-47 |
| Msa0476680 | Msa1449260 | 0.800434 | 1.443594e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449260 | MtrunA17_Chr2g0322651 | 95.556 | 135 | 6 | 0 | 1 | 135 | 1 | 135 | 6.89e-90 | 256 |
| Msa1449260 | MtrunA17_Chr4g0024531 | 40.541 | 148 | 69 | 6 | 1 | 134 | 1 | 143 | 3.15e-19 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTACTACCTATAGCTTTA+TGG | 0.181508 | tig0032473:+23783 | None:intergenic |
| ATGCTATCTTCTCTCCTTGA+TGG | 0.225457 | tig0032473:+24087 | None:intergenic |
| CATATTGTGCTGATGATATT+TGG | 0.253195 | tig0032473:+24154 | None:intergenic |
| TCTTCTCTCCTTGATGGTTT+AGG | 0.273383 | tig0032473:+24093 | None:intergenic |
| TGGTGTTAAGAATACTTCTT+TGG | 0.346243 | tig0032473:-23923 | Msa1449260:CDS |
| CTTCTACAAATGGGATTATG+AGG | 0.369074 | tig0032473:-24008 | Msa1449260:CDS |
| GATGGTAATTCTTCTACAAA+TGG | 0.380283 | tig0032473:-24018 | Msa1449260:CDS |
| AAAATACAAGATGGTAATAA+AGG | 0.383448 | tig0032473:-24045 | Msa1449260:CDS |
| TGAGCAAGGAAGAGTTGAAA+AGG | 0.433978 | tig0032473:-23972 | Msa1449260:CDS |
| GGATCACTCCATAAAGCTAT+AGG | 0.447618 | tig0032473:-23791 | None:intergenic |
| AGCTTTATGGAGTGATCCTC+AGG | 0.453586 | tig0032473:+23796 | None:intergenic |
| ATGGTAATTCTTCTACAAAT+GGG | 0.455241 | tig0032473:-24017 | Msa1449260:CDS |
| TGTTAAGAATACTTCTTTGG+AGG | 0.491214 | tig0032473:-23920 | Msa1449260:CDS |
| AGAAGATAGCATGAAGAAGA+AGG | 0.497833 | tig0032473:-24076 | Msa1449260:CDS |
| AGGGTTGAAGAAATTGATGA+TGG | 0.510389 | tig0032473:-23856 | Msa1449260:CDS |
| ATAGCATGAAGAAGAAGGTA+AGG | 0.516436 | tig0032473:-24071 | Msa1449260:CDS |
| AGCAATAAGAACATTGAGAA+TGG | 0.527142 | tig0032473:-23943 | Msa1449260:CDS |
| GATGGTAATAAAGGAAGTGA+TGG | 0.535398 | tig0032473:-24036 | Msa1449260:CDS |
| GATTAAGCTAGTTGTGAGCA+AGG | 0.543965 | tig0032473:-23986 | Msa1449260:CDS |
| GGTTGAAGAAATTGATGATG+GGG | 0.548126 | tig0032473:-23854 | Msa1449260:CDS |
| GAGCAAGGAAGAGTTGAAAA+GGG | 0.552525 | tig0032473:-23971 | Msa1449260:CDS |
| GGGTTGAAGAAATTGATGAT+GGG | 0.553313 | tig0032473:-23855 | Msa1449260:CDS |
| TCAGGAATACTATCTAAAGC+TGG | 0.566598 | tig0032473:+23814 | None:intergenic |
| GTTGAAGAAATTGATGATGG+GGG | 0.576047 | tig0032473:-23853 | Msa1449260:CDS |
| AAAGAAAGTTGAGAAGATGA+AGG | 0.577557 | tig0032473:-24118 | Msa1449260:CDS |
| TGAAGGCACCTAAACCATCA+AGG | 0.604079 | tig0032473:-24101 | Msa1449260:CDS |
| GTAAGGTTCAAAATACAAGA+TGG | 0.613431 | tig0032473:-24054 | Msa1449260:CDS |
| AGCTTTAGATAGTATTCCTG+AGG | 0.616536 | tig0032473:-23812 | Msa1449260:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATACAAGATGGTAATAA+AGG | - | tig0032473:23924-23943 | Msa1449260:CDS | 20.0% |
| ! | ATGGTAATTCTTCTACAAAT+GGG | - | tig0032473:23952-23971 | Msa1449260:CDS | 25.0% |
| ! | CATATTGTGCTGATGATATT+TGG | + | tig0032473:23818-23837 | None:intergenic | 30.0% |
| AAAGAAAGTTGAGAAGATGA+AGG | - | tig0032473:23851-23870 | Msa1449260:CDS | 30.0% | |
| GTAAGGTTCAAAATACAAGA+TGG | - | tig0032473:23915-23934 | Msa1449260:CDS | 30.0% | |
| GATGGTAATTCTTCTACAAA+TGG | - | tig0032473:23951-23970 | Msa1449260:CDS | 30.0% | |
| AGCAATAAGAACATTGAGAA+TGG | - | tig0032473:24026-24045 | Msa1449260:CDS | 30.0% | |
| ! | TGGTGTTAAGAATACTTCTT+TGG | - | tig0032473:24046-24065 | Msa1449260:CDS | 30.0% |
| TGTTAAGAATACTTCTTTGG+AGG | - | tig0032473:24049-24068 | Msa1449260:CDS | 30.0% | |
| ! | TGAAAGAAAAAAGTGTGTCT+AGG | - | tig0032473:24093-24112 | Msa1449260:CDS | 30.0% |
| ! | GAAAGAAAAAAGTGTGTCTA+GGG | - | tig0032473:24094-24113 | Msa1449260:CDS | 30.0% |
| AGAAGATAGCATGAAGAAGA+AGG | - | tig0032473:23893-23912 | Msa1449260:CDS | 35.0% | |
| ATAGCATGAAGAAGAAGGTA+AGG | - | tig0032473:23898-23917 | Msa1449260:CDS | 35.0% | |
| GATGGTAATAAAGGAAGTGA+TGG | - | tig0032473:23933-23952 | Msa1449260:CDS | 35.0% | |
| CTTCTACAAATGGGATTATG+AGG | - | tig0032473:23961-23980 | Msa1449260:CDS | 35.0% | |
| AGGGTTGAAGAAATTGATGA+TGG | - | tig0032473:24113-24132 | Msa1449260:CDS | 35.0% | |
| ! | GGGTTGAAGAAATTGATGAT+GGG | - | tig0032473:24114-24133 | Msa1449260:CDS | 35.0% |
| !! | GGTTGAAGAAATTGATGATG+GGG | - | tig0032473:24115-24134 | Msa1449260:CDS | 35.0% |
| !! | GTTGAAGAAATTGATGATGG+GGG | - | tig0032473:24116-24135 | Msa1449260:CDS | 35.0% |
| ! | TCAGGAATACTATCTAAAGC+TGG | + | tig0032473:24158-24177 | None:intergenic | 35.0% |
| !! | AGCTTTAGATAGTATTCCTG+AGG | - | tig0032473:24157-24176 | Msa1449260:CDS | 35.0% |
| ! | TCTTCTCTCCTTGATGGTTT+AGG | + | tig0032473:23879-23898 | None:intergenic | 40.0% |
| ATGCTATCTTCTCTCCTTGA+TGG | + | tig0032473:23885-23904 | None:intergenic | 40.0% | |
| GATTAAGCTAGTTGTGAGCA+AGG | - | tig0032473:23983-24002 | Msa1449260:CDS | 40.0% | |
| TGAGCAAGGAAGAGTTGAAA+AGG | - | tig0032473:23997-24016 | Msa1449260:CDS | 40.0% | |
| GAGCAAGGAAGAGTTGAAAA+GGG | - | tig0032473:23998-24017 | Msa1449260:CDS | 40.0% | |
| ! | ATGATGGGGGATTAGATTCT+TGG | - | tig0032473:24129-24148 | Msa1449260:CDS | 40.0% |
| !! | TGAAGGCACCTAAACCATCA+AGG | - | tig0032473:23868-23887 | Msa1449260:CDS | 45.0% |
| ATGGGGGATTAGATTCTTGG+AGG | - | tig0032473:24132-24151 | Msa1449260:CDS | 45.0% | |
| ! | AGCTTTATGGAGTGATCCTC+AGG | + | tig0032473:24176-24195 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0032473 | gene | 23792 | 24199 | 23792 | ID=Msa1449260;Name=Msa1449260 |
| tig0032473 | mRNA | 23792 | 24199 | 23792 | ID=Msa1449260-mRNA-1;Parent=Msa1449260;Name=Msa1449260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|135 |
| tig0032473 | exon | 23792 | 24199 | 23792 | ID=Msa1449260-mRNA-1:exon:16874;Parent=Msa1449260-mRNA-1 |
| tig0032473 | CDS | 23792 | 24199 | 23792 | ID=Msa1449260-mRNA-1:cds;Parent=Msa1449260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
