AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1450810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450810 MtrunA17_Chr2g0287651 40.521 691 358 7 98 749 68 744 5.92e-172 523
Msa1450810 MtrunA17_Chr1g0203051 32.997 594 345 6 68 614 34 621 2.35e-103 336
Msa1450810 MtrunA17_Chr1g0202341 31.336 584 350 9 104 642 50 627 4.62e-89 299
Msa1450810 MtrunA17_Chr5g0448761 31.524 571 334 8 109 629 64 627 2.52e-85 291
Msa1450810 MtrunA17_Chr3g0082121 30.217 599 373 11 108 670 46 635 5.28e-78 269
Msa1450810 MtrunA17_Chr2g0324011 28.475 590 362 8 109 648 14 593 5.56e-77 263
Msa1450810 MtrunA17_Chr2g0296781 28.254 676 408 16 109 730 1 653 1.14e-75 263
Msa1450810 MtrunA17_Chr6g0458391 28.413 542 360 9 109 627 229 765 1.15e-73 258
Msa1450810 MtrunA17_Chr8g0363841 26.989 641 397 13 101 682 9 637 1.17e-73 256
Msa1450810 MtrunA17_Chr3g0080351 28.476 597 380 13 100 665 71 651 1.74e-73 259
Msa1450810 MtrunA17_Chr1g0198761 29.688 576 342 10 111 631 53 620 6.16e-73 256
Msa1450810 MtrunA17_Chr1g0203721 27.564 624 397 13 111 690 69 681 8.23e-73 258
Msa1450810 MtrunA17_Chr6g0477261 34.577 402 248 6 237 627 48 445 1.08e-72 253
Msa1450810 MtrunA17_Chr5g0441581 27.113 627 392 13 109 690 36 642 1.73e-71 251
Msa1450810 MtrunA17_Chr2g0329031 26.350 611 381 12 92 660 1 584 5.14e-70 248
Msa1450810 MtrunA17_Chr1g0207551 29.464 560 346 10 109 627 38 589 2.68e-68 244
Msa1450810 MtrunA17_Chr1g0196541 30.070 572 331 16 109 627 1 556 1.07e-66 238
Msa1450810 MtrunA17_Chr5g0448511 30.653 398 266 5 237 628 5 398 9.72e-65 228
Msa1450810 MtrunA17_Chr3g0118611 30.822 438 284 7 244 675 118 542 5.79e-64 231
Msa1450810 MtrunA17_Chr5g0440451 26.763 624 375 15 109 675 47 645 4.15e-63 231
Msa1450810 MtrunA17_Chr2g0287061 28.351 582 380 14 110 675 188 748 2.99e-60 221
Msa1450810 MtrunA17_Chr2g0276841 26.282 624 398 14 111 687 76 684 1.11e-57 211
Msa1450810 MtrunA17_Chr2g0291581 27.240 558 356 13 109 627 75 621 2.92e-57 210
Msa1450810 MtrunA17_Chr2g0291591 30.103 485 281 11 106 546 65 535 2.39e-56 204
Msa1450810 MtrunA17_Chr5g0431001 30.569 422 235 7 111 478 67 484 2.68e-56 203
Msa1450810 MtrunA17_Chr2g0290391 25.457 656 393 15 109 696 55 682 3.19e-52 195
Msa1450810 MtrunA17_Chr2g0294841 26.842 570 354 13 111 628 51 609 3.50e-52 196
Msa1450810 MtrunA17_Chr1g0164591 34.007 297 164 6 192 457 12 307 9.01e-48 173
Msa1450810 MtrunA17_Chr2g0308841 33.051 236 156 1 402 637 4 237 1.57e-38 144
Msa1450810 MtrunA17_Chr7g0258861 29.825 285 147 6 109 348 14 290 7.74e-30 120
Msa1450810 MtrunA17_Chr1g0149131 34.545 110 67 1 349 453 1 110 2.47e-16 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450810 AT4G38180.1 30.233 645 395 12 33 631 3 638 2.93e-85 291
Msa1450810 AT4G15090.1 28.999 669 413 15 57 677 2 656 6.52e-79 275
Msa1450810 AT4G15090.5 28.999 669 413 15 57 677 2 656 6.52e-79 275
Msa1450810 AT4G15090.3 28.999 669 413 15 57 677 2 656 6.52e-79 275
Msa1450810 AT4G15090.2 28.999 669 413 15 57 677 2 656 6.52e-79 275
Msa1450810 AT4G15090.4 28.999 669 413 15 57 677 2 656 6.52e-79 275
Msa1450810 AT3G22170.2 28.036 667 424 16 56 686 30 676 1.06e-73 260
Msa1450810 AT3G22170.1 28.036 667 424 16 56 686 30 676 1.06e-73 260
Msa1450810 AT2G27110.2 27.316 637 396 14 63 669 14 613 1.50e-69 249
Msa1450810 AT2G27110.1 27.316 637 396 14 63 669 14 613 1.50e-69 249
Msa1450810 AT4G19990.7 27.931 580 379 11 83 631 6 577 6.15e-66 237
Msa1450810 AT4G19990.6 28.073 545 362 9 110 631 26 563 9.75e-66 236
Msa1450810 AT4G19990.2 28.073 545 362 9 110 631 26 563 9.75e-66 236
Msa1450810 AT4G19990.3 28.073 545 362 9 110 631 26 563 1.02e-65 236
Msa1450810 AT4G19990.4 28.073 545 362 9 110 631 26 563 1.02e-65 236
Msa1450810 AT3G06250.2 27.521 585 379 12 109 675 194 751 1.09e-65 236
Msa1450810 AT3G06250.1 27.521 585 379 12 109 675 194 751 1.09e-65 236
Msa1450810 AT3G06250.3 27.521 585 379 12 109 675 194 751 1.09e-65 236
Msa1450810 AT4G19990.5 28.073 545 362 9 110 631 26 563 1.10e-65 236
Msa1450810 AT4G19990.1 28.660 485 331 7 155 631 29 506 1.09e-62 226
Msa1450810 AT1G80010.1 28.151 611 362 17 84 636 79 670 1.15e-62 226
Msa1450810 AT2G27110.3 30.324 432 281 7 244 669 51 468 2.16e-62 225
Msa1450810 AT2G32250.5 27.616 583 370 12 109 667 42 596 1.52e-61 224
Msa1450810 AT2G32250.2 27.616 583 370 12 109 667 42 596 1.59e-61 224
Msa1450810 AT2G32250.4 27.616 583 370 12 109 667 42 596 1.59e-61 224
Msa1450810 AT2G32250.6 27.616 583 370 12 109 667 42 596 1.76e-61 224
Msa1450810 AT2G32250.1 27.616 583 370 12 109 667 42 596 1.76e-61 224
Msa1450810 AT2G32250.3 27.616 583 370 12 109 667 42 596 1.76e-61 224
Msa1450810 AT2G32250.8 27.616 583 370 12 109 667 1 555 1.89e-61 224
Msa1450810 AT2G32250.7 27.616 583 370 12 109 667 1 555 2.08e-61 224
Msa1450810 AT1G76320.1 26.847 555 365 12 109 630 1 547 2.09e-61 223
Msa1450810 AT1G76320.4 26.847 555 365 12 109 630 1 547 2.09e-61 223
Msa1450810 AT1G76320.2 26.847 555 365 12 109 630 1 547 2.10e-61 223
Msa1450810 AT1G76320.3 26.847 555 365 12 109 630 1 547 2.98e-61 221
Msa1450810 AT5G18960.2 26.904 591 378 13 109 675 215 775 1.06e-60 222
Msa1450810 AT5G18960.1 26.904 591 378 13 109 675 215 775 1.06e-60 222
Msa1450810 AT1G52520.1 27.417 569 354 14 109 632 89 643 1.44e-60 220
Msa1450810 AT1G10240.2 26.498 634 384 14 111 687 54 662 3.17e-57 210
Msa1450810 AT1G10240.1 26.498 634 384 14 111 687 54 662 3.17e-57 210
Msa1450810 AT5G28530.1 26.910 576 357 11 111 633 61 625 4.82e-56 207
Msa1450810 AT4G38170.1 28.337 427 277 9 276 686 45 458 5.83e-55 200
Msa1450810 AT5G28530.3 26.736 576 354 12 111 633 61 621 6.15e-55 203
Msa1450810 AT5G28530.2 26.736 576 354 12 111 633 61 621 6.15e-55 203
Msa1450810 AT5G28530.4 24.203 533 363 11 111 633 61 562 9.96e-39 154
Msa1450810 AT5G28530.5 23.827 533 361 11 111 633 61 558 1.45e-37 150

Find 162 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACTGATCCACCATTATT+TGG 0.155571 tig0033088:+24760 Msa1450810:intergenic
CAAAGGCGATGCCTTGTATT+TGG 0.159430 tig0033088:+26506 Msa1450810:intergenic
TCAATGTTGATTGTGATATT+TGG 0.171428 tig0033088:+24997 Msa1450810:intergenic
CTCTTGCAAATCAGACTTTC+AGG 0.192125 tig0033088:+25786 Msa1450810:intergenic
TCACTCAATTTACGGTAATT+AGG 0.235603 tig0033088:+27063 Msa1450810:intergenic
TATCAACTTCACTCAATTTA+CGG 0.276736 tig0033088:+27055 Msa1450810:intergenic
ATCACTCGCAACAAATTTAC+TGG 0.280329 tig0033088:+25533 Msa1450810:intergenic
CCATCAGTAATCACCGCTTT+TGG 0.302214 tig0033088:+26566 Msa1450810:intergenic
TCCATACTTGTTCTTTCTAT+AGG 0.305560 tig0033088:+26727 Msa1450810:intergenic
TAACACCATACCCAACATTA+TGG 0.313783 tig0033088:+24809 Msa1450810:intergenic
CAGTGTTAGAATGTGATGAT+AGG 0.338987 tig0033088:+24713 Msa1450810:intergenic
AGATGATGATATAAGGGATA+TGG 0.347376 tig0033088:-27400 Msa1450810:CDS
ACCTATAGAAAGAACAAGTA+TGG 0.349144 tig0033088:-26728 Msa1450810:CDS
ACGACTTTGTTCGTACGATT+TGG 0.356788 tig0033088:-27152 Msa1450810:CDS
ACACCAATTCCAGCAAGTTC+TGG 0.360337 tig0033088:+25378 Msa1450810:intergenic
ATGTGACAGATGATGATATA+AGG 0.367162 tig0033088:-27407 Msa1450810:CDS
GAGTTTACATTTACAGATGA+TGG 0.367609 tig0033088:-26833 Msa1450810:CDS
CTCGGAACATTGTGTGTTCC+AGG 0.370631 tig0033088:+24664 Msa1450810:intergenic
TAAGAGCGCAAGTGATTATA+AGG 0.371814 tig0033088:-25634 Msa1450810:CDS
AGGTTTGACTATGAGTGTTT+TGG 0.377109 tig0033088:-26773 Msa1450810:CDS
TTACCCACCTGTTGTTTGAT+AGG 0.378005 tig0033088:+26345 Msa1450810:intergenic
TGTTCTAATTGTAGAAATCC+TGG 0.392101 tig0033088:-25420 Msa1450810:CDS
CACTATGGGGTGCCAAATAA+TGG 0.393131 tig0033088:-24772 Msa1450810:CDS
ATCCATGTTGTCATACTTCA+TGG 0.394339 tig0033088:+25815 Msa1450810:intergenic
ACTGGAACACACAATGTTCC+TGG 0.398163 tig0033088:-24682 Msa1450810:CDS
ATCATTCGCAACAAATTTGT+TGG 0.403036 tig0033088:+25506 Msa1450810:intergenic
TCTAACACTGCTCTGCATAC+TGG 0.407068 tig0033088:-24700 Msa1450810:CDS
TTTGGGCAGATTCTACTAGT+AGG 0.409763 tig0033088:-26793 Msa1450810:CDS
CCCATAGTTTCTCAATGTCT+TGG 0.412782 tig0033088:+24485 Msa1450810:intergenic
GATTATAAGGAGGCTCTAGA+AGG 0.413898 tig0033088:-25621 Msa1450810:CDS
GATTCATTATCAGACTCAAT+AGG 0.414121 tig0033088:+25324 Msa1450810:intergenic
GCACTGGTATTACACGTAAG+AGG 0.418801 tig0033088:-25116 Msa1450810:CDS
CAAGGCATCGCCTTTGCGCC+TGG 0.420887 tig0033088:-26499 Msa1450810:CDS
AACAAATACAAGCACTAGAA+AGG 0.422563 tig0033088:-25037 Msa1450810:CDS
TGTCCAGAACTTGCTGGAAT+TGG 0.422787 tig0033088:-25381 Msa1450810:CDS
AAAGTTTAAAGATGTCAAAA+AGG 0.436388 tig0033088:-26036 Msa1450810:CDS
TACGGTAATTAGGCATCAAA+TGG 0.442752 tig0033088:+27073 Msa1450810:intergenic
CGCAGAACAATTAGTTCGAA+TGG 0.445789 tig0033088:+27191 Msa1450810:intergenic
TAAATTTGTTGCGAGTGATA+AGG 0.460501 tig0033088:-25529 Msa1450810:CDS
AAATGGACATGACGAGGATA+AGG 0.462491 tig0033088:-25556 Msa1450810:CDS
ATACTTGTTCTTTCTATAGG+TGG 0.464692 tig0033088:+26730 Msa1450810:intergenic
GCGTCAATTAGTATGCAATA+AGG 0.467578 tig0033088:-27265 Msa1450810:CDS
AACGCAAAGGAGCTAATTCA+TGG 0.469712 tig0033088:+27100 Msa1450810:intergenic
AGTGTTAGAATGTGATGATA+GGG 0.470693 tig0033088:+24714 Msa1450810:intergenic
TTCGAATGGTTAGCGTAGAT+CGG 0.471186 tig0033088:+27205 Msa1450810:intergenic
AATAGCCGTCATGAGGGAAA+TGG 0.477370 tig0033088:-25573 Msa1450810:CDS
CAAGGGAAAGAGAACTTTGA+TGG 0.478759 tig0033088:-27447 Msa1450810:CDS
CGGACGTGTCCAGAACTTGC+TGG 0.483560 tig0033088:-25387 Msa1450810:CDS
GAAATCCTGGGCACACAATA+CGG 0.497533 tig0033088:-25407 Msa1450810:CDS
CACTCTGTAGCAGTGTCAAA+TGG 0.499398 tig0033088:-25159 Msa1450810:CDS
CCAAAAGCGGTGATTACTGA+TGG 0.502215 tig0033088:-26566 Msa1450810:CDS
ATGTGAAGCGTGATTCTAAT+GGG 0.504223 tig0033088:-27299 Msa1450810:CDS
TATCAGTATCAGCCTTGCCT+TGG 0.504289 tig0033088:+26870 Msa1450810:intergenic
AATGGTGTGACCCGTGACTT+TGG 0.505092 tig0033088:-24880 Msa1450810:CDS
GCAAGGGTTAAAATACAAGA+TGG 0.508614 tig0033088:-26920 Msa1450810:CDS
GTATCACTATGTACATCAAA+AGG 0.509968 tig0033088:+27678 Msa1450810:intergenic
CCACGGGTTTGAAGTAAGAA+AGG 0.512169 tig0033088:-27325 Msa1450810:CDS
TTAGAACAACATCTTGTCCT+TGG 0.513299 tig0033088:+25435 Msa1450810:intergenic
TGTGATGATAGGGTAGTTGT+CGG 0.517812 tig0033088:+24724 Msa1450810:intergenic
AGTTTGAATCTGAGTGGAAG+AGG 0.520947 tig0033088:-26385 Msa1450810:CDS
ATTTAGTCTGCAACTTGTCA+AGG 0.524883 tig0033088:+25898 Msa1450810:intergenic
CAAGGGTTAAAATACAAGAT+GGG 0.527779 tig0033088:-26919 Msa1450810:CDS
AACAACGATGTTCGTACTGA+TGG 0.529175 tig0033088:-27519 Msa1450810:CDS
AAAGTCTGATTTGCAAGAGA+TGG 0.531904 tig0033088:-25782 Msa1450810:CDS
GATGTGAAGCGTGATTCTAA+TGG 0.532287 tig0033088:-27300 Msa1450810:CDS
GTTCTAATTGTAGAAATCCT+GGG 0.533951 tig0033088:-25419 Msa1450810:CDS
AAGTGCCCCTCGACGAAACA+TGG 0.534361 tig0033088:+25692 Msa1450810:intergenic
CACGTCCGTATTGTGTGCCC+AGG 0.534840 tig0033088:+25402 Msa1450810:intergenic
ACAAATACAAGCACTAGAAA+GGG 0.535750 tig0033088:-25036 Msa1450810:CDS
AGCATTTCGATGCAAATGCC+AGG 0.536176 tig0033088:+26481 Msa1450810:intergenic
GTAGTTGTCGGAGGGAATTG+TGG 0.539742 tig0033088:+24736 Msa1450810:intergenic
TTTATCGGTTGAAGAGAATG+TGG 0.541414 tig0033088:-26316 Msa1450810:CDS
GAGTTGTGTTTCCAAATACA+AGG 0.541485 tig0033088:-26517 Msa1450810:CDS
AATAAGGAGGGTGTTAATGA+TGG 0.543414 tig0033088:-27249 Msa1450810:CDS
AAGACAGCCATTGTGCAAGT+TGG 0.545484 tig0033088:-24834 Msa1450810:CDS
AGCTAATTCATGGTTGTGAG+AGG 0.545517 tig0033088:+27110 Msa1450810:intergenic
ACTTCATGGCACAAATAATG+TGG 0.547507 tig0033088:+25829 Msa1450810:intergenic
CAAAAGCGGTGATTACTGAT+GGG 0.550752 tig0033088:-26565 Msa1450810:CDS
TTGACCTATCAAACAACAGG+TGG 0.553778 tig0033088:-26349 Msa1450810:CDS
GATGCAAATGCCAGGCGCAA+AGG 0.554082 tig0033088:+26489 Msa1450810:intergenic
TCGTCATTTGCCTCCCAAGA+AGG 0.555407 tig0033088:-25732 Msa1450810:CDS
GTTTGAATCTGAGTGGAAGA+GGG 0.557830 tig0033088:-26384 Msa1450810:CDS
CACTGGTATTACACGTAAGA+GGG 0.559146 tig0033088:-25115 Msa1450810:CDS
GGTGTCGTGTCTGAAGATCA+AGG 0.560464 tig0033088:-25360 Msa1450810:CDS
CGAGATGTTGAGAAAAGCAC+TGG 0.563759 tig0033088:-25132 Msa1450810:CDS
TTCATCATTACCTTCTTGGG+AGG 0.565482 tig0033088:+25722 Msa1450810:intergenic
GTCTGAAGATCAAGGGGAGA+AGG 0.567087 tig0033088:-25352 Msa1450810:CDS
TATTGTTTGCAAAATACCAC+GGG 0.573778 tig0033088:-27341 Msa1450810:CDS
CCTTTGACATTCACCATCAG+TGG 0.574175 tig0033088:+25483 Msa1450810:intergenic
GTGGAATTTAACCATGGTAA+TGG 0.576772 tig0033088:-27483 Msa1450810:CDS
GCACAAATAATGTGGGAACA+TGG 0.578248 tig0033088:+25837 Msa1450810:intergenic
GACATTGATAGTGATACAGA+TGG 0.581147 tig0033088:-27621 Msa1450810:CDS
TGTGACAGATGATGATATAA+GGG 0.581249 tig0033088:-27406 Msa1450810:CDS
AAATGACCATGTTTCGTCGA+GGG 0.581274 tig0033088:-25698 Msa1450810:CDS
GTGTCGTGTCTGAAGATCAA+GGG 0.581492 tig0033088:-25359 Msa1450810:CDS
GCTAATTCATGGTTGTGAGA+GGG 0.581523 tig0033088:+27111 Msa1450810:intergenic
TCAATTAGTATGCAATAAGG+AGG 0.584633 tig0033088:-27262 Msa1450810:CDS
AATGACCATGTTTCGTCGAG+GGG 0.585541 tig0033088:-25697 Msa1450810:CDS
GCAAGTTGGAACCATAATGT+TGG 0.585606 tig0033088:-24820 Msa1450810:CDS
TAAGATAAATAGCCGTCATG+AGG 0.585792 tig0033088:-25580 Msa1450810:CDS
GAAGCTTCAAAGATATTCAC+CGG 0.587720 tig0033088:-26062 Msa1450810:CDS
AAGATAAATAGCCGTCATGA+GGG 0.587755 tig0033088:-25579 Msa1450810:CDS
ATGATAGGGTAGTTGTCGGA+GGG 0.591320 tig0033088:+24728 Msa1450810:intergenic
TGGAACCATAATGTTGGGTA+TGG 0.594448 tig0033088:-24814 Msa1450810:CDS
CTTCATGGCACAAATAATGT+GGG 0.594948 tig0033088:+25830 Msa1450810:intergenic
TTATCGGTTGAAGAGAATGT+GGG 0.595180 tig0033088:-26315 Msa1450810:CDS
TATGAGTGCGTTGAAGAATG+TGG 0.597255 tig0033088:-24543 Msa1450810:CDS
TGATAGTGATACAGATGGGG+TGG 0.597988 tig0033088:-27616 Msa1450810:CDS
GAACGAGATGAAGATGAGGA+TGG 0.598765 tig0033088:-25981 Msa1450810:CDS
TTTGAGTTACTTCCAAGGCA+AGG 0.598828 tig0033088:-26882 Msa1450810:CDS
GTATTGTTTGCAAAATACCA+CGG 0.599210 tig0033088:-27342 Msa1450810:CDS
CAAGTTGGAACCATAATGTT+GGG 0.599464 tig0033088:-24819 Msa1450810:CDS
GTCAATGCAGTTGTCAGCAT+AGG 0.607551 tig0033088:+26098 Msa1450810:intergenic
CTGAAAAGTTTGAATCTGAG+TGG 0.609008 tig0033088:-26391 Msa1450810:CDS
AAGGGTTAAAATACAAGATG+GGG 0.610552 tig0033088:-26918 Msa1450810:CDS
AAACATCGCAGTGACTATTG+TGG 0.611836 tig0033088:-24509 Msa1450810:CDS
ATTATAAGGAGGCTCTAGAA+GGG 0.613050 tig0033088:-25620 Msa1450810:CDS
GCTACTTTGAGTTACTTCCA+AGG 0.616840 tig0033088:-26887 Msa1450810:CDS
GTTGATAAACAAGTTGCTAG+TGG 0.617676 tig0033088:-27039 Msa1450810:CDS
CATGTCCATTTCCCTCATGA+CGG 0.619225 tig0033088:+25568 Msa1450810:intergenic
GATGATAGGGTAGTTGTCGG+AGG 0.619255 tig0033088:+24727 Msa1450810:intergenic
AAATGGTTGTTTCTCCATGG+TGG 0.622494 tig0033088:+26675 Msa1450810:intergenic
CATTGAACGAGATGAAGATG+AGG 0.623154 tig0033088:-25985 Msa1450810:CDS
ACATAGTGATACAGATGAGG+TGG 0.623452 tig0033088:-27667 Msa1450810:CDS
CCACTGATGGTGAATGTCAA+AGG 0.624002 tig0033088:-25483 Msa1450810:CDS
TATGGTTCCAACTTGCACAA+TGG 0.627946 tig0033088:+24827 Msa1450810:intergenic
ATACTCACCGATGTATAACT+CGG 0.636787 tig0033088:+24646 Msa1450810:intergenic
AAAAGCGGTGATTACTGATG+GGG 0.637407 tig0033088:-26564 Msa1450810:CDS
TTTAGTGGATATAACCACCA+TGG 0.638528 tig0033088:-26689 Msa1450810:CDS
AGAACGACATCAATCTGTGA+AGG 0.639278 tig0033088:-26254 Msa1450810:CDS
CCTTTCTTACTTCAAACCCG+TGG 0.639832 tig0033088:+27325 Msa1450810:intergenic
CATTGCAAACACCATTACCA+TGG 0.642035 tig0033088:+27472 Msa1450810:intergenic
TATGGGGTGCCAAATAATGG+TGG 0.646881 tig0033088:-24769 Msa1450810:CDS
AATGGTGTTTGCAATGAGCA+AGG 0.648235 tig0033088:-27465 Msa1450810:CDS
GCTAGTGGTCTACATTCGCA+AGG 0.649023 tig0033088:-27024 Msa1450810:CDS
AACAATAGAGACATACAAGT+GGG 0.649289 tig0033088:-26627 Msa1450810:CDS
ATCAAATGGACAAAACGCAA+AGG 0.651947 tig0033088:+27087 Msa1450810:intergenic
AAAATGACCATGTTTCGTCG+AGG 0.652304 tig0033088:-25699 Msa1450810:CDS
TGACCTATCAAACAACAGGT+GGG 0.653955 tig0033088:-26348 Msa1450810:CDS
ACATTGATAGTGATACAGAT+GGG 0.654877 tig0033088:-27620 Msa1450810:CDS
ATGGTGTTTGCAATGAGCAA+GGG 0.657603 tig0033088:-27464 Msa1450810:CDS
TCTGTAGCAGTGTCAAATGG+TGG 0.657609 tig0033088:-25156 Msa1450810:CDS
TGATGGAGATGCAGACGGTG+TGG 0.660310 tig0033088:-27502 Msa1450810:CDS
TGTCGTGTCTGAAGATCAAG+GGG 0.668190 tig0033088:-25358 Msa1450810:CDS
AAACAATAGAGACATACAAG+TGG 0.671084 tig0033088:-26628 Msa1450810:CDS
TGCCATGAAGTATGACAACA+TGG 0.671427 tig0033088:-25817 Msa1450810:CDS
GTATGACAAACATCCAAAAG+CGG 0.671446 tig0033088:-26579 Msa1450810:CDS
TGAATGTCAAAGGTGCCCGT+AGG 0.674674 tig0033088:-25473 Msa1450810:CDS
CAATTAGTATGCAATAAGGA+GGG 0.675920 tig0033088:-27261 Msa1450810:CDS
CAATGTTCCGAGTTATACAT+CGG 0.680199 tig0033088:-24653 Msa1450810:intron
CATTTGACACTGCTACAGAG+TGG 0.682675 tig0033088:+25160 Msa1450810:intergenic
GAGCGCAAGTGATTATAAGG+AGG 0.683366 tig0033088:-25631 Msa1450810:CDS
TGTTGCGAATGATCCACTGA+TGG 0.687875 tig0033088:-25496 Msa1450810:CDS
GACGGTGTGGAATTTAACCA+TGG 0.688761 tig0033088:-27489 Msa1450810:CDS
TGAGGGAAATGGACATGACG+AGG 0.691164 tig0033088:-25562 Msa1450810:CDS
TGTAGACGCTTTGAACGCAG+AGG 0.705116 tig0033088:-25864 Msa1450810:CDS
CGTACTGATGGAGATGCAGA+CGG 0.711480 tig0033088:-27507 Msa1450810:CDS
ACATAGACTATTGAACGCAG+CGG 0.718507 tig0033088:+25668 Msa1450810:intergenic
CATTGATAGTGATACAGATG+GGG 0.737791 tig0033088:-27619 Msa1450810:CDS
TGTACATAGTGATACAGATG+AGG 0.739686 tig0033088:-27670 Msa1450810:CDS
AATTATGCAAGAAATCCACA+AGG 0.743577 tig0033088:+26189 Msa1450810:intergenic
GGATATGGAATTCAACACAG+AGG 0.763384 tig0033088:-27385 Msa1450810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAATTGTATATGATATTT+TGG - tig0033088:26970-26989 Msa1450810:intron 15.0%
!!! AATTATTTTAATATGCACTA+TGG - tig0033088:27414-27433 Msa1450810:CDS 15.0%
!!! ATTATTTTAATATGCACTAT+GGG - tig0033088:27415-27434 Msa1450810:CDS 15.0%
!!! TCATAAACTGTTTTAAATAT+AGG + tig0033088:27643-27662 Msa1450810:intergenic 15.0%
!! AAAGTTTAAAGATGTCAAAA+AGG - tig0033088:26165-26184 Msa1450810:CDS 20.0%
!!! ATTTTCAAGGTTAATGTATT+TGG - tig0033088:26253-26272 Msa1450810:CDS 20.0%
!!! TTATTTTAATATGCACTATG+GGG - tig0033088:27416-27435 Msa1450810:CDS 20.0%
! GATGGAAAAAATAAGAAGAA+TGG - tig0033088:24970-24989 Msa1450810:intron 25.0%
! TATCAACTTCACTCAATTTA+CGG + tig0033088:25149-25168 Msa1450810:intergenic 25.0%
! GAAGGACTTATATAATTTCA+CGG - tig0033088:25250-25269 Msa1450810:intron 25.0%
! ATATCCACTAAAAATGACAA+GGG + tig0033088:25504-25523 Msa1450810:intergenic 25.0%
! TATATCCACTAAAAATGACA+AGG + tig0033088:25505-25524 Msa1450810:intergenic 25.0%
! AATTAAAGCACAAGCAAAAA+TGG + tig0033088:25546-25565 Msa1450810:intergenic 25.0%
!!! GGTTCTAAATTTGTTTTCAA+AGG - tig0033088:25595-25614 Msa1450810:CDS 25.0%
! GGTGGGTAAAAAAAATTTAT+CGG - tig0033088:25870-25889 Msa1450810:CDS 25.0%
!! GACATCTTTAAACTTTTTAC+CGG + tig0033088:26161-26180 Msa1450810:intergenic 25.0%
!! CAAAAAAGATGTAGCTTTTT+CGG - tig0033088:26276-26295 Msa1450810:CDS 25.0%
!! TTTTTCATCATTACCTTCTT+GGG + tig0033088:26485-26504 Msa1450810:intergenic 25.0%
!!! TTTTTTCATCATTACCTTCT+TGG + tig0033088:26486-26505 Msa1450810:intergenic 25.0%
!! TCAATGTTGATTGTGATATT+TGG + tig0033088:27207-27226 Msa1450810:intergenic 25.0%
! CAGCAAATATACTATAACAA+AGG + tig0033088:27240-27259 Msa1450810:intergenic 25.0%
!!! TTGTTTTGATTGTTGTAGAA+TGG - tig0033088:27303-27322 Msa1450810:CDS 25.0%
GTATCACTATGTACATCAAA+AGG + tig0033088:24526-24545 Msa0880280:intergenic 30.0%
!! ACATTGATAGTGATACAGAT+GGG - tig0033088:24581-24600 Msa1450810:intron 30.0%
ATGTGACAGATGATGATATA+AGG - tig0033088:24794-24813 Msa1450810:CDS 30.0%
TGTGACAGATGATGATATAA+GGG - tig0033088:24795-24814 Msa1450810:CDS 30.0%
AGATGATGATATAAGGGATA+TGG - tig0033088:24801-24820 Msa1450810:CDS 30.0%
GTATTGTTTGCAAAATACCA+CGG - tig0033088:24859-24878 Msa1450810:CDS 30.0%
TATTGTTTGCAAAATACCAC+GGG - tig0033088:24860-24879 Msa1450810:CDS 30.0%
TCAATTAGTATGCAATAAGG+AGG - tig0033088:24939-24958 Msa1450810:intron 30.0%
CAATTAGTATGCAATAAGGA+GGG - tig0033088:24940-24959 Msa1450810:intron 30.0%
TCACTCAATTTACGGTAATT+AGG + tig0033088:25141-25160 Msa1450810:intergenic 30.0%
TGTAAGAACATGTCATATCT+TGG - tig0033088:25200-25219 Msa1450810:intron 30.0%
GTAAGAACATGTCATATCTT+GGG - tig0033088:25201-25220 Msa1450810:intron 30.0%
!!! TCATATCTTGGGTTTTTTGA+AGG - tig0033088:25212-25231 Msa1450810:intron 30.0%
ATATAATTTCACGGATCATC+AGG - tig0033088:25259-25278 Msa1450810:intron 30.0%
CAAGGGTTAAAATACAAGAT+GGG - tig0033088:25282-25301 Msa1450810:intron 30.0%
AAGGGTTAAAATACAAGATG+GGG - tig0033088:25283-25302 Msa1450810:intron 30.0%
GAGTTTACATTTACAGATGA+TGG - tig0033088:25368-25387 Msa1450810:CDS 30.0%
!!! ACGCCTAGAAAACATTTTTT+GGG - tig0033088:25391-25410 Msa1450810:CDS 30.0%
ATACTTGTTCTTTCTATAGG+TGG + tig0033088:25474-25493 Msa1450810:intergenic 30.0%
ACCTATAGAAAGAACAAGTA+TGG - tig0033088:25473-25492 Msa1450810:CDS 30.0%
TCCATACTTGTTCTTTCTAT+AGG + tig0033088:25477-25496 Msa1450810:intergenic 30.0%
TATCCACTAAAAATGACAAG+GGG + tig0033088:25503-25522 Msa1450810:intergenic 30.0%
AAACAATAGAGACATACAAG+TGG - tig0033088:25573-25592 Msa1450810:CDS 30.0%
AACAATAGAGACATACAAGT+GGG - tig0033088:25574-25593 Msa1450810:CDS 30.0%
! TTTTGGATGTTTGTCATACA+TGG + tig0033088:25621-25640 Msa1450810:intergenic 30.0%
!! ATTTTGACCTATCAAACAAC+AGG - tig0033088:25849-25868 Msa1450810:CDS 30.0%
TTCTAACACCACACAAAAAA+TGG + tig0033088:25931-25950 Msa1450810:intergenic 30.0%
AATTATGCAAGAAATCCACA+AGG + tig0033088:26015-26034 Msa1450810:intergenic 30.0%
!!! TGGAAACATTGTCATTTTCA+AGG - tig0033088:26240-26259 Msa1450810:CDS 30.0%
!! TAAATTTGTTGCGAGTGATA+AGG - tig0033088:26672-26691 Msa1450810:CDS 30.0%
ATCATTCGCAACAAATTTGT+TGG + tig0033088:26698-26717 Msa1450810:intergenic 30.0%
TGTTCTAATTGTAGAAATCC+TGG - tig0033088:26781-26800 Msa1450810:CDS 30.0%
GTTCTAATTGTAGAAATCCT+GGG - tig0033088:26782-26801 Msa1450810:CDS 30.0%
GATTCATTATCAGACTCAAT+AGG + tig0033088:26880-26899 Msa1450810:intergenic 30.0%
AACAAATACAAGCACTAGAA+AGG - tig0033088:27164-27183 Msa1450810:CDS 30.0%
ACAAATACAAGCACTAGAAA+GGG - tig0033088:27165-27184 Msa1450810:CDS 30.0%
! ATTTTTCATGTCCAAAGTCA+CGG + tig0033088:27335-27354 Msa1450810:intergenic 30.0%
AAAACTGATCCACCATTATT+TGG + tig0033088:27444-27463 Msa1450810:intergenic 30.0%
AGTGTTAGAATGTGATGATA+GGG + tig0033088:27490-27509 Msa1450810:intergenic 30.0%
TGTACATAGTGATACAGATG+AGG - tig0033088:24531-24550 Msa1450810:CDS 35.0%
!! GACATTGATAGTGATACAGA+TGG - tig0033088:24580-24599 Msa1450810:intron 35.0%
!! CATTGATAGTGATACAGATG+GGG - tig0033088:24582-24601 Msa1450810:intron 35.0%
GTGGAATTTAACCATGGTAA+TGG - tig0033088:24718-24737 Msa1450810:CDS 35.0%
ATGTGAAGCGTGATTCTAAT+GGG - tig0033088:24902-24921 Msa1450810:intron 35.0%
GCGTCAATTAGTATGCAATA+AGG - tig0033088:24936-24955 Msa1450810:intron 35.0%
!! AATAAGGAGGGTGTTAATGA+TGG - tig0033088:24952-24971 Msa1450810:intron 35.0%
ATCAAATGGACAAAACGCAA+AGG + tig0033088:25117-25136 Msa1450810:intergenic 35.0%
TACGGTAATTAGGCATCAAA+TGG + tig0033088:25131-25150 Msa1450810:intergenic 35.0%
! GTTGATAAACAAGTTGCTAG+TGG - tig0033088:25162-25181 Msa1450810:CDS 35.0%
!!! TTGGGTTTTTTGAAGGACTT+GGG - tig0033088:25219-25238 Msa1450810:intron 35.0%
GCAAGGGTTAAAATACAAGA+TGG - tig0033088:25281-25300 Msa1450810:intron 35.0%
!!! GACGCCTAGAAAACATTTTT+TGG - tig0033088:25390-25409 Msa1450810:CDS 35.0%
! CTGCCCAAAAAATGTTTTCT+AGG + tig0033088:25397-25416 Msa1450810:intergenic 35.0%
! AGGTTTGACTATGAGTGTTT+TGG - tig0033088:25428-25447 Msa1450810:CDS 35.0%
!!! AAACCCCTTGTCATTTTTAG+TGG - tig0033088:25497-25516 Msa1450810:CDS 35.0%
! TTTAGTGGATATAACCACCA+TGG - tig0033088:25512-25531 Msa1450810:CDS 35.0%
!! CAAAAATGGTTGTTTCTCCA+TGG + tig0033088:25532-25551 Msa1450810:intergenic 35.0%
GTATGACAAACATCCAAAAG+CGG - tig0033088:25622-25641 Msa1450810:CDS 35.0%
GAGTTGTGTTTCCAAATACA+AGG - tig0033088:25684-25703 Msa1450810:CDS 35.0%
!!! TCAAACTTTTCAGGTGTACA+AGG + tig0033088:25803-25822 Msa1450810:intergenic 35.0%
! CACTCAGATTCAAACTTTTC+AGG + tig0033088:25812-25831 Msa1450810:intergenic 35.0%
CTGAAAAGTTTGAATCTGAG+TGG - tig0033088:25810-25829 Msa1450810:CDS 35.0%
! TTTATCGGTTGAAGAGAATG+TGG - tig0033088:25885-25904 Msa1450810:CDS 35.0%
! TTATCGGTTGAAGAGAATGT+GGG - tig0033088:25886-25905 Msa1450810:CDS 35.0%
GAAGCTTCAAAGATATTCAC+CGG - tig0033088:26139-26158 Msa1450810:CDS 35.0%
ATTTAGTCTGCAACTTGTCA+AGG + tig0033088:26306-26325 Msa1450810:intergenic 35.0%
CTTCATGGCACAAATAATGT+GGG + tig0033088:26374-26393 Msa1450810:intergenic 35.0%
ACTTCATGGCACAAATAATG+TGG + tig0033088:26375-26394 Msa1450810:intergenic 35.0%
ATCCATGTTGTCATACTTCA+TGG + tig0033088:26389-26408 Msa1450810:intergenic 35.0%
! AAAGTCTGATTTGCAAGAGA+TGG - tig0033088:26419-26438 Msa1450810:CDS 35.0%
! TAAGAGCGCAAGTGATTATA+AGG - tig0033088:26567-26586 Msa1450810:CDS 35.0%
!! ATTATAAGGAGGCTCTAGAA+GGG - tig0033088:26581-26600 Msa1450810:CDS 35.0%
TAAGATAAATAGCCGTCATG+AGG - tig0033088:26621-26640 Msa1450810:CDS 35.0%
AAGATAAATAGCCGTCATGA+GGG - tig0033088:26622-26641 Msa1450810:CDS 35.0%
ATCACTCGCAACAAATTTAC+TGG + tig0033088:26671-26690 Msa1450810:intergenic 35.0%
!!! TGTTGACTTTTTACTCCTAC+GGG + tig0033088:26746-26765 Msa1450810:intergenic 35.0%
!!! GTGTTGACTTTTTACTCCTA+CGG + tig0033088:26747-26766 Msa1450810:intergenic 35.0%
TTAGAACAACATCTTGTCCT+TGG + tig0033088:26769-26788 Msa1450810:intergenic 35.0%
TATCATTACTCATAGACAGC+TGG + tig0033088:27008-27027 Msa1450810:intergenic 35.0%
!! TGCTGAAAATTGTGTTGCTT+TGG - tig0033088:27255-27274 Msa1450810:CDS 35.0%
! TTTTTCATGTCCAAAGTCAC+GGG + tig0033088:27334-27353 Msa1450810:intergenic 35.0%
! CAAGTTGGAACCATAATGTT+GGG - tig0033088:27382-27401 Msa1450810:CDS 35.0%
TAACACCATACCCAACATTA+TGG + tig0033088:27395-27414 Msa1450810:intergenic 35.0%
CAGTGTTAGAATGTGATGAT+AGG + tig0033088:27491-27510 Msa1450810:intergenic 35.0%
CAATGTTCCGAGTTATACAT+CGG - tig0033088:27548-27567 Msa1450810:CDS 35.0%
ATACTCACCGATGTATAACT+CGG + tig0033088:27558-27577 Msa1450810:intergenic 35.0%
ACATAGTGATACAGATGAGG+TGG - tig0033088:24534-24553 Msa1450810:CDS 40.0%
AACAACGATGTTCGTACTGA+TGG - tig0033088:24682-24701 Msa1450810:CDS 40.0%
CATTGCAAACACCATTACCA+TGG + tig0033088:24732-24751 Msa1450810:intergenic 40.0%
AATGGTGTTTGCAATGAGCA+AGG - tig0033088:24736-24755 Msa1450810:CDS 40.0%
ATGGTGTTTGCAATGAGCAA+GGG - tig0033088:24737-24756 Msa1450810:CDS 40.0%
CAAGGGAAAGAGAACTTTGA+TGG - tig0033088:24754-24773 Msa1450810:CDS 40.0%
GGATATGGAATTCAACACAG+AGG - tig0033088:24816-24835 Msa1450810:CDS 40.0%
GATGTGAAGCGTGATTCTAA+TGG - tig0033088:24901-24920 Msa1450810:intron 40.0%
TTCGAATGGTTAGCGTAGAT+CGG + tig0033088:24999-25018 Msa1450810:intergenic 40.0%
CGCAGAACAATTAGTTCGAA+TGG + tig0033088:25013-25032 Msa1450810:intergenic 40.0%
ACGACTTTGTTCGTACGATT+TGG - tig0033088:25049-25068 Msa1450810:CDS 40.0%
GCTAATTCATGGTTGTGAGA+GGG + tig0033088:25093-25112 Msa1450810:intergenic 40.0%
AGCTAATTCATGGTTGTGAG+AGG + tig0033088:25094-25113 Msa1450810:intergenic 40.0%
AACGCAAAGGAGCTAATTCA+TGG + tig0033088:25104-25123 Msa1450810:intergenic 40.0%
!!! CTTGGGTTTTTTGAAGGACT+TGG - tig0033088:25218-25237 Msa1450810:intron 40.0%
AGGACTTGGGTTTATCAAGA+AGG - tig0033088:25232-25251 Msa1450810:intron 40.0%
! GCTACTTTGAGTTACTTCCA+AGG - tig0033088:25314-25333 Msa1450810:CDS 40.0%
! TTTGAGTTACTTCCAAGGCA+AGG - tig0033088:25319-25338 Msa1450810:CDS 40.0%
TTTGGGCAGATTCTACTAGT+AGG - tig0033088:25408-25427 Msa1450810:CDS 40.0%
!! AAATGGTTGTTTCTCCATGG+TGG + tig0033088:25529-25548 Msa1450810:intergenic 40.0%
CAAAAGCGGTGATTACTGAT+GGG - tig0033088:25636-25655 Msa1450810:CDS 40.0%
AAAAGCGGTGATTACTGATG+GGG - tig0033088:25637-25656 Msa1450810:CDS 40.0%
AGTTTGAATCTGAGTGGAAG+AGG - tig0033088:25816-25835 Msa1450810:CDS 40.0%
GTTTGAATCTGAGTGGAAGA+GGG - tig0033088:25817-25836 Msa1450810:CDS 40.0%
TTGACCTATCAAACAACAGG+TGG - tig0033088:25852-25871 Msa1450810:CDS 40.0%
TGACCTATCAAACAACAGGT+GGG - tig0033088:25853-25872 Msa1450810:CDS 40.0%
! TTACCCACCTGTTGTTTGAT+AGG + tig0033088:25859-25878 Msa1450810:intergenic 40.0%
!!! ATGCGAGACCATTTTTTGTG+TGG - tig0033088:25920-25939 Msa1450810:CDS 40.0%
AGAACGACATCAATCTGTGA+AGG - tig0033088:25947-25966 Msa1450810:CDS 40.0%
AGATGTCAAAAAGGCGATAG+AGG - tig0033088:26174-26193 Msa1450810:CDS 40.0%
CATTGAACGAGATGAAGATG+AGG - tig0033088:26216-26235 Msa1450810:CDS 40.0%
GCACAAATAATGTGGGAACA+TGG + tig0033088:26367-26386 Msa1450810:intergenic 40.0%
TGCCATGAAGTATGACAACA+TGG - tig0033088:26384-26403 Msa1450810:CDS 40.0%
CTCTTGCAAATCAGACTTTC+AGG + tig0033088:26418-26437 Msa1450810:intergenic 40.0%
TTCATCATTACCTTCTTGGG+AGG + tig0033088:26482-26501 Msa1450810:intergenic 40.0%
AAAATGACCATGTTTCGTCG+AGG - tig0033088:26502-26521 Msa1450810:CDS 40.0%
AAATGACCATGTTTCGTCGA+GGG - tig0033088:26503-26522 Msa1450810:CDS 40.0%
ACATAGACTATTGAACGCAG+CGG + tig0033088:26536-26555 Msa1450810:intergenic 40.0%
!! GATTATAAGGAGGCTCTAGA+AGG - tig0033088:26580-26599 Msa1450810:CDS 40.0%
AAATGGACATGACGAGGATA+AGG - tig0033088:26645-26664 Msa1450810:CDS 40.0%
GGAGTAAAAAGTCAACACCA+AGG - tig0033088:26749-26768 Msa1450810:CDS 40.0%
CACTGGTATTACACGTAAGA+GGG - tig0033088:27086-27105 Msa1450810:CDS 40.0%
TATGGTTCCAACTTGCACAA+TGG + tig0033088:27377-27396 Msa1450810:intergenic 40.0%
! GCAAGTTGGAACCATAATGT+TGG - tig0033088:27381-27400 Msa1450810:CDS 40.0%
TGGAACCATAATGTTGGGTA+TGG - tig0033088:27387-27406 Msa1450810:CDS 40.0%
TGTGATGATAGGGTAGTTGT+CGG + tig0033088:27480-27499 Msa1450810:intergenic 40.0%
TATGAGTGCGTTGAAGAATG+TGG - tig0033088:27658-27677 Msa1450810:CDS 40.0%
AAACATCGCAGTGACTATTG+TGG - tig0033088:27692-27711 Msa1450810:CDS 40.0%
! TGATAGTGATACAGATGGGG+TGG - tig0033088:24585-24604 Msa1450810:intron 45.0%
GACGGTGTGGAATTTAACCA+TGG - tig0033088:24712-24731 Msa1450810:CDS 45.0%
CCTTTCTTACTTCAAACCCG+TGG + tig0033088:24879-24898 Msa1450810:intergenic 45.0%
! CCACGGGTTTGAAGTAAGAA+AGG - tig0033088:24876-24895 Msa1450810:CDS 45.0%
ATTTCACGGATCATCAGGCA+AGG - tig0033088:25264-25283 Msa1450810:intron 45.0%
TTTCACGGATCATCAGGCAA+GGG - tig0033088:25265-25284 Msa1450810:intron 45.0%
TATCAGTATCAGCCTTGCCT+TGG + tig0033088:25334-25353 Msa1450810:intergenic 45.0%
! CCATCAGTAATCACCGCTTT+TGG + tig0033088:25638-25657 Msa1450810:intergenic 45.0%
CCAAAAGCGGTGATTACTGA+TGG - tig0033088:25635-25654 Msa1450810:CDS 45.0%
CAAAGGCGATGCCTTGTATT+TGG + tig0033088:25698-25717 Msa1450810:intergenic 45.0%
AGCATTTCGATGCAAATGCC+AGG + tig0033088:25723-25742 Msa1450810:intergenic 45.0%
!!! CCACAAGGCTGTTTTTGTGT+CGG + tig0033088:26000-26019 Msa1450810:intergenic 45.0%
GTCAATGCAGTTGTCAGCAT+AGG + tig0033088:26106-26125 Msa1450810:intergenic 45.0%
GAACGAGATGAAGATGAGGA+TGG - tig0033088:26220-26239 Msa1450810:CDS 45.0%
AATGACCATGTTTCGTCGAG+GGG - tig0033088:26504-26523 Msa1450810:CDS 45.0%
GAGCGCAAGTGATTATAAGG+AGG - tig0033088:26570-26589 Msa1450810:CDS 45.0%
AATAGCCGTCATGAGGGAAA+TGG - tig0033088:26628-26647 Msa1450810:CDS 45.0%
CATGTCCATTTCCCTCATGA+CGG + tig0033088:26636-26655 Msa1450810:intergenic 45.0%
TGTTGCGAATGATCCACTGA+TGG - tig0033088:26705-26724 Msa1450810:CDS 45.0%
CCTTTGACATTCACCATCAG+TGG + tig0033088:26721-26740 Msa1450810:intergenic 45.0%
! CCACTGATGGTGAATGTCAA+AGG - tig0033088:26718-26737 Msa1450810:CDS 45.0%
GAAATCCTGGGCACACAATA+CGG - tig0033088:26794-26813 Msa1450810:CDS 45.0%
!! TGTCCAGAACTTGCTGGAAT+TGG - tig0033088:26820-26839 Msa1450810:CDS 45.0%
ACACCAATTCCAGCAAGTTC+TGG + tig0033088:26826-26845 Msa1450810:intergenic 45.0%
GTGTCGTGTCTGAAGATCAA+GGG - tig0033088:26842-26861 Msa1450810:CDS 45.0%
TGTCGTGTCTGAAGATCAAG+GGG - tig0033088:26843-26862 Msa1450810:CDS 45.0%
CATTTGACACTGCTACAGAG+TGG + tig0033088:27044-27063 Msa1450810:intergenic 45.0%
CACTCTGTAGCAGTGTCAAA+TGG - tig0033088:27042-27061 Msa1450810:CDS 45.0%
TCTGTAGCAGTGTCAAATGG+TGG - tig0033088:27045-27064 Msa1450810:CDS 45.0%
! CGAGATGTTGAGAAAAGCAC+TGG - tig0033088:27069-27088 Msa1450810:CDS 45.0%
! GCACTGGTATTACACGTAAG+AGG - tig0033088:27085-27104 Msa1450810:CDS 45.0%
AAGACAGCCATTGTGCAAGT+TGG - tig0033088:27367-27386 Msa1450810:CDS 45.0%
! CACTATGGGGTGCCAAATAA+TGG - tig0033088:27429-27448 Msa1450810:CDS 45.0%
! TATGGGGTGCCAAATAATGG+TGG - tig0033088:27432-27451 Msa1450810:CDS 45.0%
ATGATAGGGTAGTTGTCGGA+GGG + tig0033088:27476-27495 Msa1450810:intergenic 45.0%
TCTAACACTGCTCTGCATAC+TGG - tig0033088:27501-27520 Msa1450810:CDS 45.0%
ACTGGAACACACAATGTTCC+TGG - tig0033088:27519-27538 Msa1450810:CDS 45.0%
! CGTACTGATGGAGATGCAGA+CGG - tig0033088:24694-24713 Msa1450810:CDS 50.0%
GCTAGTGGTCTACATTCGCA+AGG - tig0033088:25177-25196 Msa1450810:CDS 50.0%
CCGACACAAAAACAGCCTTG+TGG - tig0033088:25997-26016 Msa1450810:CDS 50.0%
! TGTAGACGCTTTGAACGCAG+AGG - tig0033088:26337-26356 Msa1450810:CDS 50.0%
TCGTCATTTGCCTCCCAAGA+AGG - tig0033088:26469-26488 Msa1450810:CDS 50.0%
TGAGGGAAATGGACATGACG+AGG - tig0033088:26639-26658 Msa1450810:CDS 50.0%
!! TGAATGTCAAAGGTGCCCGT+AGG - tig0033088:26728-26747 Msa1450810:CDS 50.0%
! GGTGTCGTGTCTGAAGATCA+AGG - tig0033088:26841-26860 Msa1450810:CDS 50.0%
GTCTGAAGATCAAGGGGAGA+AGG - tig0033088:26849-26868 Msa1450810:CDS 50.0%
AATGGTGTGACCCGTGACTT+TGG - tig0033088:27321-27340 Msa1450810:CDS 50.0%
GTAGTTGTCGGAGGGAATTG+TGG + tig0033088:27468-27487 Msa1450810:intergenic 50.0%
GATGATAGGGTAGTTGTCGG+AGG + tig0033088:27477-27496 Msa1450810:intergenic 50.0%
CTCGGAACATTGTGTGTTCC+AGG + tig0033088:27540-27559 Msa1450810:intergenic 50.0%
TGATGGAGATGCAGACGGTG+TGG - tig0033088:24699-24718 Msa1450810:CDS 55.0%
GATGCAAATGCCAGGCGCAA+AGG + tig0033088:25715-25734 Msa1450810:intergenic 55.0%
AAGTGCCCCTCGACGAAACA+TGG + tig0033088:26512-26531 Msa1450810:intergenic 55.0%
CACGTCCGTATTGTGTGCCC+AGG + tig0033088:26802-26821 Msa1450810:intergenic 60.0%
CGGACGTGTCCAGAACTTGC+TGG - tig0033088:26814-26833 Msa1450810:CDS 60.0%
!! CAAGGCATCGCCTTTGCGCC+TGG - tig0033088:25702-25721 Msa1450810:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0033088 gene 24496 27727 24496 ID=Msa1450810;Name=Msa1450810
tig0033088 mRNA 24496 27727 24496 ID=Msa1450810-mRNA-1;Parent=Msa1450810;Name=Msa1450810-mRNA-1;_AED=0.34;_eAED=0.35;_QI=0|0|0|0.4|1|1|5|0|959
tig0033088 exon 27023 27727 27023 ID=Msa1450810-mRNA-1:exon:17679;Parent=Msa1450810-mRNA-1
tig0033088 exon 25311 26942 25311 ID=Msa1450810-mRNA-1:exon:17678;Parent=Msa1450810-mRNA-1
tig0033088 exon 24993 25214 24993 ID=Msa1450810-mRNA-1:exon:17677;Parent=Msa1450810-mRNA-1
tig0033088 exon 24654 24902 24654 ID=Msa1450810-mRNA-1:exon:17676;Parent=Msa1450810-mRNA-1
tig0033088 exon 24496 24567 24496 ID=Msa1450810-mRNA-1:exon:17675;Parent=Msa1450810-mRNA-1
tig0033088 CDS 27023 27727 27023 ID=Msa1450810-mRNA-1:cds;Parent=Msa1450810-mRNA-1
tig0033088 CDS 25311 26942 25311 ID=Msa1450810-mRNA-1:cds;Parent=Msa1450810-mRNA-1
tig0033088 CDS 24993 25214 24993 ID=Msa1450810-mRNA-1:cds;Parent=Msa1450810-mRNA-1
tig0033088 CDS 24654 24902 24654 ID=Msa1450810-mRNA-1:cds;Parent=Msa1450810-mRNA-1
tig0033088 CDS 24496 24567 24496 ID=Msa1450810-mRNA-1:cds;Parent=Msa1450810-mRNA-1
Gene Sequence

>Msa1450810

ATGTCTACATATAGTGAAGCAACATTGCCTTTTGATGTACATAGTGATACAGATGAGGTGGAATTTAATAGTGTTAATGATATTGACATTGATAGTGATACAGATGGGGTGGAATTTAATAGTGTTAATGATGTTGACACTGATAGTGACACTGATAGTGATACTGAAGATATAAAAGTCAATAGCAACAACGATGTTCGTACTGATGGAGATGCAGACGGTGTGGAATTTAACCATGGTAATGGTGTTTGCAATGAGCAAGGGAAAGAGAACTTTGATGGCTACAAATGCATCAGTGATGTGACAGATGATGATATAAGGGATATGGAATTCAACACAGAGGATGAAGCTATAAAGTTTTATGTATTGTTTGCAAAATACCACGGGTTTGAAGTAAGAAAGGATGATGTGAAGCGTGATTCTAATGGGAACATTATTATGCGTCAATTAGTATGCAATAAGGAGGGTGTTAATGATGGAAAAAATAAGAAGAATGGCAGCCGATCTACGCTAACCATTCGAACTAATTGTTCTGCGAAACTTCGAGTAGTCTACGACTTTGTTCGTACGATTTGGAGAGTTTCTATTTTCAAACCCTCTCACAACCATGAATTAGCTCCTTTGCGTTTTGTCCATTTGATGCCTAATTACCGTAAATTGAGTGAAGTTGATAAACAAGTTGCTAGTGGTCTACATTCGCAAGGTGCAAGGGTTAAAATACAAGATGGGGATACTTTTGCTACTTTGAGTTACTTCCAAGGCAAGGCTGATACTGATACATCATTCTTTTCAGAGTTTACATTTACAGATGATGGACGCCTAGAAAACATTTTTTGGGCAGATTCTACTAGTAGGTTTGACTATGAGTGTTTTGGCGATGTTGTTGCATTTGACACCACCTATAGAAAGAACAAGTATGGAAAACCCCTTGTCATTTTTAGTGGATATAACCACCATGGAGAAACAACCATTTTTGCTTGTGCTTTAATTTGTGATGAAACAATAGAGACATACAAGTGGGTTCTAAATTTGTTTTCAAAGGCCATGTATGACAAACATCCAAAAGCGGTGATTACTGATGGGGATAAATCAATGAGAAAAGCTATCAGAGTTGTGTTTCCAAATACAAGGCATCGCCTTTGCGCCTGGCATTTGCATCGAAATGCTGTTCAACATGTGAAGAATTCAAAATTTTTAGAAGAGTTCAAGTCACTGATGTATTCTCCTTGTACACCTGAAAAGTTTGAATCTGAGTGGAAGAGGGTTGTTGAAAATTTTGACCTATCAAACAACAGGTGGGTAAAAAAAATTTATCGGTTGAAGAGAATGTGGGCAAGTGCATACATGCGAGACCATTTTTTGTGTGGTGTTAGAACGACATCAATCTGTGAAGGAGTGAATTCATTTATCAAGAAGTATGTCCGACACAAAAACAGCCTTGTGGATTTCTTGCATAATTTTGAAAGAGCTCTCAAAGAATACAGACATAATGAATTACTGTCTGATTTCAAGTCGTTCTATTATCATCCTATGCTGACAACTGCATTGACTACTTTTGAGTCTGAAGCTTCAAAGATATTCACCGGTAAAAAGTTTAAAGATGTCAAAAAGGCGATAGAGGATGTTGTTGCATTATCAGTCATTGAACGAGATGAAGATGAGGATGGAAACATTGTCATTTTCAAGGTTAATGTATTTGGCAAAAAAGATGTAGCTTTTTCGGTATACCTTGACAAGTTGCAGACTAAATTTTTATGTGATTGTAGACGCTTTGAACGCAGAGGACTGCCATGTTCCCACATTATTTGTGCCATGAAGTATGACAACATGGATGTGTTTCCTGAAAGTCTGATTTGCAAGAGATGGACAAAGACTGCAAAGAGTGATCACATCTCGTCATTTGCCTCCCAAGAAGGTAATGATGAAAAAATGACCATGTTTCGTCGAGGGGCACTTGCCGCTGCGTTCAATAGTCTATGTGATGTTTCGTGTAAGAGCGCAAGTGATTATAAGGAGGCTCTAGAAGGGATTTATAGTCTGTGTAATAAGATAAATAGCCGTCATGAGGGAAATGGACATGACGAGGATAAGGCCAGTAAATTTGTTGCGAGTGATAAGGCCAACAAATTTGTTGCGAATGATCCACTGATGGTGAATGTCAAAGGTGCCCGTAGGAGTAAAAAGTCAACACCAAGGACAAGATGTTGTTCTAATTGTAGAAATCCTGGGCACACAATACGGACGTGTCCAGAACTTGCTGGAATTGGTGTCGTGTCTGAAGATCAAGGGGAGAAGGCTTCACCTATTGAGTCTGATAATGAATCAAAAAGTTTAAGTCTGTCTATGAGTAATGATATGAATGATGACATCCACTCTGTAGCAGTGTCAAATGGTGGACGAGATGTTGAGAAAAGCACTGGTATTACACGTAAGAGGGAATGTGACAATGTTGCATTTTCAACACAAGACTCTGTCAACAAAAGAAAAACAAAAACAAATACAAGCACTAGAAAGGGTGATGCTTGTGTGACTCCAAATATCACAATCAACATTGATAATAATGGTGTGACCCGTGACTTTGGACATGAAAAATCTACTCTCAAACAAGACAGCCATTGTGCAAGTTGGAACCATAATGTTGGGTATGGTGTTAATTATTTTAATATGCACTATGGGGTGCCAAATAATGGTGGATCAGTTTTTCCACAATTCCCTCCGACAACTACCCTATCATCACATTCTAACACTGCTCTGCATACTGGAACACACAATGTTCCTGGAACACACAATGTTCCGAGTTATACATCGTTTATGAGTGCGTTGAAGAATGTGGAGCTGTTAGCAAAACATCGCAGTGACTATTGTGGACCAAGACATTGA
Protein sequence

>Msa1450810

MSTYSEATLPFDVHSDTDEVEFNSVNDIDIDSDTDGVEFNSVNDVDTDSDTDSDTEDIKVNSNNDVRTDGDADGVEFNHGNGVCNEQGKENFDGYKCISDVTDDDIRDMEFNTEDEAIKFYVLFAKYHGFEVRKDDVKRDSNGNIIMRQLVCNKEGVNDGKNKKNGSRSTLTIRTNCSAKLRVVYDFVRTIWRVSIFKPSHNHELAPLRFVHLMPNYRKLSEVDKQVASGLHSQGARVKIQDGDTFATLSYFQGKADTDTSFFSEFTFTDDGRLENIFWADSTSRFDYECFGDVVAFDTTYRKNKYGKPLVIFSGYNHHGETTIFACALICDETIETYKWVLNLFSKAMYDKHPKAVITDGDKSMRKAIRVVFPNTRHRLCAWHLHRNAVQHVKNSKFLEEFKSLMYSPCTPEKFESEWKRVVENFDLSNNRWVKKIYRLKRMWASAYMRDHFLCGVRTTSICEGVNSFIKKYVRHKNSLVDFLHNFERALKEYRHNELLSDFKSFYYHPMLTTALTTFESEASKIFTGKKFKDVKKAIEDVVALSVIERDEDEDGNIVIFKVNVFGKKDVAFSVYLDKLQTKFLCDCRRFERRGLPCSHIICAMKYDNMDVFPESLICKRWTKTAKSDHISSFASQEGNDEKMTMFRRGALAAAFNSLCDVSCKSASDYKEALEGIYSLCNKINSRHEGNGHDEDKASKFVASDKANKFVANDPLMVNVKGARRSKKSTPRTRCCSNCRNPGHTIRTCPELAGIGVVSEDQGEKASPIESDNESKSLSLSMSNDMNDDIHSVAVSNGGRDVEKSTGITRKRECDNVAFSTQDSVNKRKTKTNTSTRKGDACVTPNITINIDNNGVTRDFGHEKSTLKQDSHCASWNHNVGYGVNYFNMHYGVPNNGGSVFPQFPPTTTLSSHSNTALHTGTHNVPGTHNVPSYTSFMSALKNVELLAKHRSDYCGPRH*