AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1345310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1345310 MtrunA17_Chr2g0287651 42.960 696 378 6 93 783 63 744 0.0 586
Msa1345310 MtrunA17_Chr1g0203051 34.564 596 367 6 68 648 34 621 1.83e-117 374
Msa1345310 MtrunA17_Chr1g0202341 32.709 587 372 10 104 676 50 627 3.77e-97 321
Msa1345310 MtrunA17_Chr5g0448761 32.807 570 362 9 109 663 64 627 4.78e-94 315
Msa1345310 MtrunA17_Chr2g0324011 29.181 586 397 6 109 682 14 593 4.44e-86 288
Msa1345310 MtrunA17_Chr2g0296781 29.204 678 433 15 109 764 1 653 3.27e-84 287
Msa1345310 MtrunA17_Chr8g0363841 28.169 639 422 11 101 714 9 635 5.43e-84 285
Msa1345310 MtrunA17_Chr5g0441581 28.346 635 408 12 109 724 36 642 1.52e-82 281
Msa1345310 MtrunA17_Chr1g0198761 31.023 577 367 10 111 665 53 620 7.42e-81 278
Msa1345310 MtrunA17_Chr3g0082121 30.099 608 396 12 108 704 46 635 7.18e-80 274
Msa1345310 MtrunA17_Chr6g0477261 34.813 428 264 6 245 661 22 445 3.64e-78 268
Msa1345310 MtrunA17_Chr1g0203721 27.885 624 429 11 111 724 69 681 5.76e-77 270
Msa1345310 MtrunA17_Chr1g0207551 30.838 561 371 9 109 661 38 589 2.68e-75 264
Msa1345310 MtrunA17_Chr6g0458391 28.042 567 364 11 109 661 229 765 5.78e-73 256
Msa1345310 MtrunA17_Chr3g0080351 27.885 624 383 13 100 699 71 651 1.52e-71 254
Msa1345310 MtrunA17_Chr1g0196541 30.594 572 362 15 109 661 1 556 2.73e-70 248
Msa1345310 MtrunA17_Chr2g0329031 26.094 617 409 11 92 694 1 584 1.99e-69 246
Msa1345310 MtrunA17_Chr5g0440451 27.404 624 405 16 109 709 47 645 4.61e-65 236
Msa1345310 MtrunA17_Chr3g0118611 29.862 509 325 11 211 709 56 542 8.77e-65 233
Msa1345310 MtrunA17_Chr5g0448511 30.653 398 266 5 271 662 5 398 9.35e-65 228
Msa1345310 MtrunA17_Chr2g0276841 27.360 625 424 14 111 721 76 684 1.06e-63 229
Msa1345310 MtrunA17_Chr5g0431001 32.151 423 261 8 111 512 67 484 3.38e-63 223
Msa1345310 MtrunA17_Chr2g0290391 26.596 658 417 16 109 730 55 682 2.22e-61 222
Msa1345310 MtrunA17_Chr2g0291581 27.592 569 374 13 109 661 75 621 4.69e-60 218
Msa1345310 MtrunA17_Chr2g0287061 27.723 606 387 14 110 709 188 748 1.03e-59 219
Msa1345310 MtrunA17_Chr2g0291591 30.247 486 313 10 106 580 65 535 6.66e-58 209
Msa1345310 MtrunA17_Chr2g0294841 28.196 571 379 14 111 662 51 609 7.86e-58 213
Msa1345310 MtrunA17_Chr1g0164591 32.459 305 192 3 192 491 12 307 2.32e-50 181
Msa1345310 MtrunA17_Chr2g0308841 33.051 236 156 1 436 671 4 237 1.99e-38 144
Msa1345310 MtrunA17_Chr7g0258861 31.579 285 176 5 109 382 14 290 9.90e-38 144
Msa1345310 MtrunA17_Chr1g0149131 35.455 110 66 1 383 487 1 110 5.60e-17 78.2
Msa1345310 MtrunA17_Chr8g0340801 33.945 109 70 1 213 321 1 107 4.01e-14 69.3
Msa1345310 MtrunA17_Chr6g0451441 25.600 250 170 6 31 269 2 246 7.75e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1345310 AT4G38180.1 31.424 646 420 12 33 665 3 638 7.32e-94 315
Msa1345310 AT4G15090.1 30.000 670 439 15 57 711 2 656 1.57e-85 293
Msa1345310 AT4G15090.5 30.000 670 439 15 57 711 2 656 1.57e-85 293
Msa1345310 AT4G15090.3 30.000 670 439 15 57 711 2 656 1.57e-85 293
Msa1345310 AT4G15090.2 30.000 670 439 15 57 711 2 656 1.57e-85 293
Msa1345310 AT4G15090.4 30.000 670 439 15 57 711 2 656 1.57e-85 293
Msa1345310 AT3G22170.2 26.163 688 444 13 56 720 30 676 5.14e-72 256
Msa1345310 AT3G22170.1 26.163 688 444 13 56 720 30 676 5.14e-72 256
Msa1345310 AT2G27110.2 26.453 654 414 15 63 703 14 613 1.87e-67 243
Msa1345310 AT2G27110.1 26.453 654 414 15 63 703 14 613 1.87e-67 243
Msa1345310 AT5G28530.1 28.423 577 381 11 111 667 61 625 1.42e-64 231
Msa1345310 AT3G06250.2 27.558 606 386 12 109 709 194 751 2.95e-64 232
Msa1345310 AT3G06250.1 27.558 606 386 12 109 709 194 751 2.95e-64 232
Msa1345310 AT3G06250.3 27.558 606 386 12 109 709 194 751 2.95e-64 232
Msa1345310 AT1G10240.2 27.373 632 409 13 111 718 54 659 8.92e-64 229
Msa1345310 AT1G10240.1 27.373 632 409 13 111 718 54 659 8.92e-64 229
Msa1345310 AT5G28530.3 28.250 577 378 12 111 667 61 621 2.47e-63 228
Msa1345310 AT5G28530.2 28.250 577 378 12 111 667 61 621 2.47e-63 228
Msa1345310 AT1G76320.2 26.702 573 377 12 109 664 1 547 6.48e-63 228
Msa1345310 AT1G76320.1 26.702 573 377 12 109 664 1 547 8.10e-63 227
Msa1345310 AT1G76320.4 26.702 573 377 12 109 664 1 547 8.10e-63 227
Msa1345310 AT1G76320.3 26.702 573 377 12 109 664 1 547 1.03e-62 226
Msa1345310 AT2G27110.3 30.787 432 279 8 278 703 51 468 2.92e-62 225
Msa1345310 AT4G19990.6 27.903 577 356 13 110 665 26 563 8.06e-62 225
Msa1345310 AT4G19990.2 27.903 577 356 13 110 665 26 563 8.06e-62 225
Msa1345310 AT4G19990.7 27.778 612 373 15 83 665 6 577 8.47e-62 225
Msa1345310 AT4G19990.3 27.903 577 356 13 110 665 26 563 1.10e-61 224
Msa1345310 AT4G19990.4 27.903 577 356 13 110 665 26 563 1.10e-61 224
Msa1345310 AT4G19990.5 27.903 577 356 13 110 665 26 563 1.41e-61 224
Msa1345310 AT1G52520.1 27.604 576 378 13 109 666 89 643 1.89e-61 223
Msa1345310 AT1G80010.1 27.994 618 388 20 84 670 79 670 5.07e-61 222
Msa1345310 AT5G18960.2 26.733 606 394 13 109 709 215 775 5.86e-60 220
Msa1345310 AT5G18960.1 26.733 606 394 13 109 709 215 775 5.86e-60 220
Msa1345310 AT4G19990.1 30.326 399 270 5 273 665 110 506 3.10e-59 216
Msa1345310 AT2G32250.6 26.742 617 366 14 109 701 42 596 3.81e-56 209
Msa1345310 AT2G32250.1 26.742 617 366 14 109 701 42 596 3.81e-56 209
Msa1345310 AT2G32250.3 26.742 617 366 14 109 701 42 596 3.81e-56 209
Msa1345310 AT2G32250.8 26.742 617 366 14 109 701 1 555 4.10e-56 208
Msa1345310 AT2G32250.2 26.742 617 366 14 109 701 42 596 4.13e-56 209
Msa1345310 AT2G32250.4 26.742 617 366 14 109 701 42 596 4.13e-56 209
Msa1345310 AT2G32250.5 26.904 617 365 14 109 701 42 596 4.69e-56 208
Msa1345310 AT2G32250.7 26.742 617 366 14 109 701 1 555 5.56e-56 208
Msa1345310 AT4G38170.1 28.337 427 277 9 310 720 45 458 5.68e-55 201
Msa1345310 AT5G28530.4 25.397 567 348 14 111 667 61 562 9.13e-44 169
Msa1345310 AT5G28530.5 25.044 567 346 14 111 667 61 558 1.76e-42 165

Find 165 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGCGATGCCTTGTATT+TGG 0.159430 tig0007116:+13096 None:intergenic
TCAATGTTGATTGTGATATT+TGG 0.171428 tig0007116:+11587 None:intergenic
CTCTTGCAAATCAGACTTTC+AGG 0.192125 tig0007116:+12376 None:intergenic
TCACTCAATTTACGGTAATT+AGG 0.235603 tig0007116:+13675 None:intergenic
CCCATAGTTTCTCAATGTTT+TGG 0.242135 tig0007116:+11073 None:intergenic
TATCAACTTCACTCAATTTA+CGG 0.276736 tig0007116:+13667 None:intergenic
ATCACTCGCAACAAATTTAC+TGG 0.280329 tig0007116:+12123 None:intergenic
CCATCAGTAATCACCGCTTT+TGG 0.302214 tig0007116:+13156 None:intergenic
TCCATACTTGTTCTTTCTAT+AGG 0.305560 tig0007116:+13317 None:intergenic
AGGACTTGGGTTTATCAAAA+AGG 0.326599 tig0007116:-13559 Msa1345310:CDS
CAGTGTTAGAATGTGATGAT+AGG 0.338987 tig0007116:+11301 None:intergenic
TGATAAACCCAAGTCCTTCA+TGG 0.346028 tig0007116:+13565 None:intergenic
AGATGATGATATAAGGGATA+TGG 0.347376 tig0007116:-14012 Msa1345310:CDS
ACCTATAGAAAGAACAAGTA+TGG 0.349144 tig0007116:-13318 Msa1345310:CDS
ACGACTTTGTTCGTACGATT+TGG 0.356788 tig0007116:-13764 Msa1345310:CDS
ACACCAATTCCAGCAAGTTC+TGG 0.360337 tig0007116:+11968 None:intergenic
ATGTGACAGATGATGATATA+AGG 0.367162 tig0007116:-14019 Msa1345310:CDS
GAGTTTACATTTACAGATGA+TGG 0.367609 tig0007116:-13423 Msa1345310:CDS
CTCGGAACATTGTGTGTTCC+AGG 0.370631 tig0007116:+11252 None:intergenic
TAAGAGCGCAAGTGATTATA+AGG 0.371814 tig0007116:-12224 Msa1345310:CDS
AGGTTTGACTATGAGTGTTT+TGG 0.377109 tig0007116:-13363 Msa1345310:CDS
TTACCCACCTGTTGTTTGAT+AGG 0.378005 tig0007116:+12935 None:intergenic
TGTAAGAACATGTCATATCT+TGG 0.387474 tig0007116:-13613 Msa1345310:CDS
TGTTCTAATTGTAGAAATCC+TGG 0.392101 tig0007116:-12010 Msa1345310:CDS
ATCCATGTTGTCATACTTCA+TGG 0.394339 tig0007116:+12405 None:intergenic
ACTGGAACACACAATGTTCC+TGG 0.398163 tig0007116:-11270 Msa1345310:CDS
ATCATTCGCAACAAATTTGT+TGG 0.403036 tig0007116:+12096 None:intergenic
TCTAACACTGCTCTGCATAC+TGG 0.407068 tig0007116:-11288 Msa1345310:CDS
ATATAATTTCACGGATCATC+AGG 0.409639 tig0007116:-13532 Msa1345310:CDS
TTTGGGCAGATTCTACTAGT+AGG 0.409763 tig0007116:-13383 Msa1345310:CDS
GATTATAAGGAGGCTCTAGA+AGG 0.413898 tig0007116:-12211 Msa1345310:CDS
GATTCATTATCAGACTCAAT+AGG 0.414121 tig0007116:+11914 None:intergenic
GCACTGGTATTACACGTAAG+AGG 0.418801 tig0007116:-11706 Msa1345310:CDS
CAAGGCATCGCCTTTGCGCC+TGG 0.420887 tig0007116:-13089 Msa1345310:CDS
AACAAATACAAGCACTAGAA+AGG 0.422563 tig0007116:-11627 Msa1345310:CDS
TGTCCAGAACTTGCTGGAAT+TGG 0.422787 tig0007116:-11971 Msa1345310:CDS
AAAGTTTAAAGATGTCAAAA+AGG 0.436388 tig0007116:-12626 Msa1345310:CDS
TACGGTAATTAGGCATCAAA+TGG 0.442752 tig0007116:+13685 None:intergenic
CGCAGAACAATTAGTTCGAA+TGG 0.445789 tig0007116:+13803 None:intergenic
AAGGGTGGCCATGAAGGACT+TGG 0.449428 tig0007116:-13573 Msa1345310:CDS
TAAATTTGTTGCGAGTGATA+AGG 0.460501 tig0007116:-12119 Msa1345310:CDS
AAATGGACATGACGAGGATA+AGG 0.462491 tig0007116:-12146 Msa1345310:CDS
ATACTTGTTCTTTCTATAGG+TGG 0.464692 tig0007116:+13320 None:intergenic
GTAAGAACATGTCATATCTT+GGG 0.464989 tig0007116:-13612 Msa1345310:CDS
GCGTCAATTAGTATGCAATA+AGG 0.467578 tig0007116:-13877 Msa1345310:CDS
AACGCAAAGGAGCTAATTCA+TGG 0.469712 tig0007116:+13712 None:intergenic
AGTGTTAGAATGTGATGATA+GGG 0.470693 tig0007116:+11302 None:intergenic
TTCGAATGGTTAGCGTAGAT+CGG 0.471186 tig0007116:+13817 None:intergenic
AATAGCCGTCATGAGGGAAA+TGG 0.477370 tig0007116:-12163 Msa1345310:CDS
CAAGGGAAAGAGAACTTTGA+TGG 0.478759 tig0007116:-14059 Msa1345310:CDS
CGGACGTGTCCAGAACTTGC+TGG 0.483560 tig0007116:-11977 Msa1345310:CDS
AAAGGACTTATATAATTTCA+CGG 0.493961 tig0007116:-13541 Msa1345310:CDS
GAAATCCTGGGCACACAATA+CGG 0.497533 tig0007116:-11997 Msa1345310:CDS
CACTCTGTAGCAGTGTCAAA+TGG 0.499398 tig0007116:-11749 Msa1345310:CDS
CCAAAAGCGGTGATTACTGA+TGG 0.502215 tig0007116:-13156 Msa1345310:CDS
ATGTGAAGCGTGATTCTAAT+GGG 0.504223 tig0007116:-13911 Msa1345310:CDS
AATGGTGTGACCCGTGACTT+TGG 0.505092 tig0007116:-11470 Msa1345310:CDS
GCAAGGGTTAAAATACAAGA+TGG 0.508614 tig0007116:-13510 Msa1345310:CDS
GTATCACTATGTACATCAAA+AGG 0.509968 tig0007116:+14290 None:intergenic
GGTCAAAAGGGTGGCCATGA+AGG 0.510809 tig0007116:-13579 Msa1345310:CDS
TTTGAGTTACTTCCAAGGTA+AGG 0.511685 tig0007116:-13472 Msa1345310:CDS
CCACGGGTTTGAAGTAAGAA+AGG 0.512169 tig0007116:-13937 Msa1345310:CDS
TTAGAACAACATCTTGTCCT+TGG 0.513299 tig0007116:+12025 None:intergenic
TGTGATGATAGGGTAGTTGT+CGG 0.517812 tig0007116:+11312 None:intergenic
AGTTTGAATCTGAGTGGAAG+AGG 0.520947 tig0007116:-12975 Msa1345310:CDS
ATTTAGTCTGCAACTTGTCA+AGG 0.524883 tig0007116:+12488 None:intergenic
CAAGGGTTAAAATACAAGAT+GGG 0.527779 tig0007116:-13509 Msa1345310:CDS
AACAACGATGTTCGTACTGA+TGG 0.529175 tig0007116:-14131 Msa1345310:CDS
AAAGTCTGATTTGCAAGAGA+TGG 0.531904 tig0007116:-12372 Msa1345310:CDS
GATGTGAAGCGTGATTCTAA+TGG 0.532287 tig0007116:-13912 Msa1345310:CDS
GTTCTAATTGTAGAAATCCT+GGG 0.533951 tig0007116:-12009 Msa1345310:CDS
AAGTGCCCCTCGACGAAACA+TGG 0.534361 tig0007116:+12282 None:intergenic
CACGTCCGTATTGTGTGCCC+AGG 0.534840 tig0007116:+11992 None:intergenic
ACAAATACAAGCACTAGAAA+GGG 0.535750 tig0007116:-11626 Msa1345310:CDS
AGCATTTCGATGCAAATGCC+AGG 0.536176 tig0007116:+13071 None:intergenic
GTAGTTGTCGGAGGGAATTG+TGG 0.539742 tig0007116:+11324 None:intergenic
TTTATCGGTTGAAGAGAATG+TGG 0.541414 tig0007116:-12906 Msa1345310:CDS
GAGTTGTGTTTCCAAATACA+AGG 0.541485 tig0007116:-13107 Msa1345310:CDS
AATAAGGAGGGTGTTAATGA+TGG 0.543414 tig0007116:-13861 Msa1345310:CDS
AGCTAATTCATGGTTGTGAG+AGG 0.545517 tig0007116:+13722 None:intergenic
TATCAGTATCAGCCTTACCT+TGG 0.546983 tig0007116:+13460 None:intergenic
ACTTCATGGCACAAATAATG+TGG 0.547507 tig0007116:+12419 None:intergenic
CAAAAGCGGTGATTACTGAT+GGG 0.550752 tig0007116:-13155 Msa1345310:CDS
TTGACCTATCAAACAACAGG+TGG 0.553778 tig0007116:-12939 Msa1345310:CDS
GATGCAAATGCCAGGCGCAA+AGG 0.554082 tig0007116:+13079 None:intergenic
TCGTCATTTGCCTCCCAAGA+AGG 0.555407 tig0007116:-12322 Msa1345310:CDS
GTTTGAATCTGAGTGGAAGA+GGG 0.557830 tig0007116:-12974 Msa1345310:CDS
CACTGGTATTACACGTAAGA+GGG 0.559146 tig0007116:-11705 Msa1345310:CDS
GGTGTCGTGTCTGAAGATCA+AGG 0.560464 tig0007116:-11950 Msa1345310:CDS
CGAGATGTTGAGAAAAGCAC+TGG 0.563759 tig0007116:-11722 Msa1345310:CDS
TTCATCATTACCTTCTTGGG+AGG 0.565482 tig0007116:+12312 None:intergenic
GTCTGAAGATCAAGGGGAGA+AGG 0.567087 tig0007116:-11942 Msa1345310:CDS
TATTGTTTGCAAAATACCAC+GGG 0.573778 tig0007116:-13953 Msa1345310:CDS
CCTTTGACATTCACCATCAG+TGG 0.574175 tig0007116:+12073 None:intergenic
GTGGAATTTAACCATGGTAA+TGG 0.576772 tig0007116:-14095 Msa1345310:CDS
GCACAAATAATGTGGGAACA+TGG 0.578248 tig0007116:+12427 None:intergenic
TGTGACAGATGATGATATAA+GGG 0.581249 tig0007116:-14018 Msa1345310:CDS
AAATGACCATGTTTCGTCGA+GGG 0.581274 tig0007116:-12288 Msa1345310:CDS
GTGTCGTGTCTGAAGATCAA+GGG 0.581492 tig0007116:-11949 Msa1345310:CDS
GCTAATTCATGGTTGTGAGA+GGG 0.581523 tig0007116:+13723 None:intergenic
TCAATTAGTATGCAATAAGG+AGG 0.584633 tig0007116:-13874 Msa1345310:CDS
AATGACCATGTTTCGTCGAG+GGG 0.585541 tig0007116:-12287 Msa1345310:CDS
TAAGATAAATAGCCGTCATG+AGG 0.585792 tig0007116:-12170 Msa1345310:CDS
AGGGTGGCCATGAAGGACTT+GGG 0.586883 tig0007116:-13572 Msa1345310:CDS
GAAGCTTCAAAGATATTCAC+CGG 0.587720 tig0007116:-12652 Msa1345310:CDS
AAGATAAATAGCCGTCATGA+GGG 0.587755 tig0007116:-12169 Msa1345310:CDS
ATACTCACCGGTGTATAACT+CGG 0.588936 tig0007116:+11234 None:intergenic
ATGATAGGGTAGTTGTCGGA+GGG 0.591320 tig0007116:+11316 None:intergenic
ATTTCACGGATCATCAGGCA+AGG 0.592596 tig0007116:-13527 Msa1345310:CDS
CTTCATGGCACAAATAATGT+GGG 0.594948 tig0007116:+12420 None:intergenic
TTATCGGTTGAAGAGAATGT+GGG 0.595180 tig0007116:-12905 Msa1345310:CDS
TATGAGTGCGTTGAAGAATG+TGG 0.597255 tig0007116:-11131 Msa1345310:CDS
GAACGAGATGAAGATGAGGA+TGG 0.598765 tig0007116:-12571 Msa1345310:CDS
GTATTGTTTGCAAAATACCA+CGG 0.599210 tig0007116:-13954 Msa1345310:CDS
GTCAATGCAGTTGTCAGCAT+AGG 0.607551 tig0007116:+12688 None:intergenic
CTGAAAAGTTTGAATCTGAG+TGG 0.609008 tig0007116:-12981 Msa1345310:CDS
AAGGGTTAAAATACAAGATG+GGG 0.610552 tig0007116:-13508 Msa1345310:CDS
AAACATCGCAGTGACTATTG+TGG 0.611836 tig0007116:-11097 Msa1345310:CDS
ATTATAAGGAGGCTCTAGAA+GGG 0.613050 tig0007116:-12210 Msa1345310:CDS
GACACTGATAGTGATACAGA+TGG 0.614810 tig0007116:-14233 Msa1345310:CDS
GCTACTTTGAGTTACTTCCA+AGG 0.617165 tig0007116:-13477 Msa1345310:CDS
GTTGATAAACAAGTTGCTAG+TGG 0.617676 tig0007116:-13651 Msa1345310:CDS
TGATAGTGATACAGATGGGG+TGG 0.618742 tig0007116:-14228 Msa1345310:CDS
CATGTCCATTTCCCTCATGA+CGG 0.619225 tig0007116:+12158 None:intergenic
GATGATAGGGTAGTTGTCGG+AGG 0.619255 tig0007116:+11315 None:intergenic
AAATGGTTGTTTCTCCATGG+TGG 0.622494 tig0007116:+13265 None:intergenic
CATTGAACGAGATGAAGATG+AGG 0.623154 tig0007116:-12575 Msa1345310:CDS
ACATAGTGATACAGATGAGG+TGG 0.623452 tig0007116:-14279 Msa1345310:CDS
CCACTGATGGTGAATGTCAA+AGG 0.624002 tig0007116:-12073 Msa1345310:CDS
TATGGTTCCAACTTGCACAA+TGG 0.627946 tig0007116:+11417 None:intergenic
TTTCACGGATCATCAGGCAA+GGG 0.637022 tig0007116:-13526 Msa1345310:CDS
AAAAGCGGTGATTACTGATG+GGG 0.637407 tig0007116:-13154 Msa1345310:CDS
TTTAGTGGATATAACCACCA+TGG 0.638528 tig0007116:-13279 Msa1345310:CDS
AGAACGACATCAATCTGTGA+AGG 0.639278 tig0007116:-12844 Msa1345310:CDS
CCTTTCTTACTTCAAACCCG+TGG 0.639832 tig0007116:+13937 None:intergenic
CATTGCAAACACCATTACCA+TGG 0.642035 tig0007116:+14084 None:intergenic
AATGGTGTTTGCAATGAGCA+AGG 0.648235 tig0007116:-14077 Msa1345310:CDS
GCTAGTGGTCTACATTCGCA+AGG 0.649023 tig0007116:-13636 Msa1345310:CDS
AACAATAGAGACATACAAGT+GGG 0.649289 tig0007116:-13217 Msa1345310:CDS
ATCAAATGGACAAAACGCAA+AGG 0.651947 tig0007116:+13699 None:intergenic
AAAATGACCATGTTTCGTCG+AGG 0.652304 tig0007116:-12289 Msa1345310:CDS
TGACCTATCAAACAACAGGT+GGG 0.653955 tig0007116:-12938 Msa1345310:CDS
ATGGTGTTTGCAATGAGCAA+GGG 0.657603 tig0007116:-14076 Msa1345310:CDS
TCTGTAGCAGTGTCAAATGG+TGG 0.657609 tig0007116:-11746 Msa1345310:CDS
TGATGGAGATGCAGACGGTG+TGG 0.660310 tig0007116:-14114 Msa1345310:CDS
ACACTGATAGTGATACAGAT+GGG 0.663559 tig0007116:-14232 Msa1345310:CDS
TGTCGTGTCTGAAGATCAAG+GGG 0.668190 tig0007116:-11948 Msa1345310:CDS
AAACAATAGAGACATACAAG+TGG 0.671084 tig0007116:-13218 Msa1345310:CDS
TGCCATGAAGTATGACAACA+TGG 0.671427 tig0007116:-12407 Msa1345310:CDS
GTATGACAAACATCCAAAAG+CGG 0.671446 tig0007116:-13169 Msa1345310:CDS
TGAATGTCAAAGGTGCCCGT+AGG 0.674674 tig0007116:-12063 Msa1345310:CDS
CAATTAGTATGCAATAAGGA+GGG 0.675920 tig0007116:-13873 Msa1345310:CDS
CAATGTTCCGAGTTATACAC+CGG 0.682160 tig0007116:-11241 Msa1345310:intron
CATTTGACACTGCTACAGAG+TGG 0.682675 tig0007116:+11750 None:intergenic
GAGCGCAAGTGATTATAAGG+AGG 0.683366 tig0007116:-12221 Msa1345310:CDS
TGTTGCGAATGATCCACTGA+TGG 0.687875 tig0007116:-12086 Msa1345310:CDS
GACGGTGTGGAATTTAACCA+TGG 0.688761 tig0007116:-14101 Msa1345310:CDS
TGAGGGAAATGGACATGACG+AGG 0.691164 tig0007116:-12152 Msa1345310:CDS
TGTAGACGCTTTGAACGCAG+AGG 0.705116 tig0007116:-12454 Msa1345310:CDS
CGTACTGATGGAGATGCAGA+CGG 0.711480 tig0007116:-14119 Msa1345310:CDS
ACATAGACTATTGAACGCAG+CGG 0.718507 tig0007116:+12258 None:intergenic
CACTGATAGTGATACAGATG+GGG 0.724418 tig0007116:-14231 Msa1345310:CDS
TGTACATAGTGATACAGATG+AGG 0.739686 tig0007116:-14282 Msa1345310:CDS
AATTATGCAAGAAATCCACA+AGG 0.743577 tig0007116:+12779 None:intergenic
GGATATGGAATTCAACACAG+AGG 0.763384 tig0007116:-13997 Msa1345310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAATTGTATATGATATTT+TGG - tig0007116:13580-13599 Msa1345310:CDS 15.0%
!!! TTATTTTAATATGCACTATA+TGG - tig0007116:14026-14045 Msa1345310:CDS 15.0%
!!! TATTTTAATATGCACTATAT+GGG - tig0007116:14027-14046 Msa1345310:CDS 15.0%
!!! TCATAAACTGTTTTAAATAT+AGG + tig0007116:14255-14274 None:intergenic 15.0%
!! AAAGGACTTATATAATTTCA+CGG - tig0007116:11860-11879 Msa1345310:intron 20.0%
!! AAAGTTTAAAGATGTCAAAA+AGG - tig0007116:12775-12794 Msa1345310:CDS 20.0%
!!! ATTTTCAAGGTTAATGTATT+TGG - tig0007116:12863-12882 Msa1345310:CDS 20.0%
!!! ATTTTAATATGCACTATATG+GGG - tig0007116:14028-14047 Msa1345310:CDS 20.0%
! GATGGAAAAAATAAGAAGAA+TGG - tig0007116:11558-11577 Msa1345310:intron 25.0%
! TATCAACTTCACTCAATTTA+CGG + tig0007116:11737-11756 None:intergenic 25.0%
! ATATCCACTAAAAATGACAA+GGG + tig0007116:12114-12133 None:intergenic 25.0%
! TATATCCACTAAAAATGACA+AGG + tig0007116:12115-12134 None:intergenic 25.0%
! AATTAAAGCACAAGCAAAAA+TGG + tig0007116:12156-12175 None:intergenic 25.0%
!!! GGTTCTAAATTTGTTTTCAA+AGG - tig0007116:12205-12224 Msa1345310:CDS 25.0%
! GGTGGGTAAAAAAAATTTAT+CGG - tig0007116:12480-12499 Msa1345310:CDS 25.0%
!! GACATCTTTAAACTTTTTAC+CGG + tig0007116:12771-12790 None:intergenic 25.0%
!! CAAAAAAGATGTAGCTTTTT+CGG - tig0007116:12886-12905 Msa1345310:CDS 25.0%
!! TTTTTCATCATTACCTTCTT+GGG + tig0007116:13095-13114 None:intergenic 25.0%
!!! TTTTTTCATCATTACCTTCT+TGG + tig0007116:13096-13115 None:intergenic 25.0%
!! TCAATGTTGATTGTGATATT+TGG + tig0007116:13817-13836 None:intergenic 25.0%
! CAGCAAATATACTATAACAA+AGG + tig0007116:13850-13869 None:intergenic 25.0%
!!! TTGTTTTGATTGTTGTAGAA+TGG - tig0007116:13913-13932 Msa1345310:CDS 25.0%
! TAAACAATCAAAATACTCAC+CGG + tig0007116:14182-14201 None:intergenic 25.0%
GTATCACTATGTACATCAAA+AGG + tig0007116:11114-11133 None:intergenic 30.0%
ATGTGACAGATGATGATATA+AGG - tig0007116:11382-11401 Msa1345310:intron 30.0%
TGTGACAGATGATGATATAA+GGG - tig0007116:11383-11402 Msa1345310:intron 30.0%
AGATGATGATATAAGGGATA+TGG - tig0007116:11389-11408 Msa1345310:intron 30.0%
GTATTGTTTGCAAAATACCA+CGG - tig0007116:11447-11466 Msa1345310:CDS 30.0%
TATTGTTTGCAAAATACCAC+GGG - tig0007116:11448-11467 Msa1345310:CDS 30.0%
TCAATTAGTATGCAATAAGG+AGG - tig0007116:11527-11546 Msa1345310:intron 30.0%
CAATTAGTATGCAATAAGGA+GGG - tig0007116:11528-11547 Msa1345310:intron 30.0%
TCACTCAATTTACGGTAATT+AGG + tig0007116:11729-11748 None:intergenic 30.0%
TGTAAGAACATGTCATATCT+TGG - tig0007116:11788-11807 Msa1345310:intron 30.0%
GTAAGAACATGTCATATCTT+GGG - tig0007116:11789-11808 Msa1345310:intron 30.0%
!!! TCATATCTTGGGTTTTTTGA+TGG - tig0007116:11800-11819 Msa1345310:intron 30.0%
!!! CATATCTTGGGTTTTTTGAT+GGG - tig0007116:11801-11820 Msa1345310:intron 30.0%
ATATAATTTCACGGATCATC+AGG - tig0007116:11869-11888 Msa1345310:intron 30.0%
CAAGGGTTAAAATACAAGAT+GGG - tig0007116:11892-11911 Msa1345310:intron 30.0%
AAGGGTTAAAATACAAGATG+GGG - tig0007116:11893-11912 Msa1345310:intron 30.0%
GAGTTTACATTTACAGATGA+TGG - tig0007116:11978-11997 Msa1345310:CDS 30.0%
!!! ACGCCTAGAAAACATTTTTT+GGG - tig0007116:12001-12020 Msa1345310:CDS 30.0%
ATACTTGTTCTTTCTATAGG+TGG + tig0007116:12084-12103 None:intergenic 30.0%
ACCTATAGAAAGAACAAGTA+TGG - tig0007116:12083-12102 Msa1345310:CDS 30.0%
TCCATACTTGTTCTTTCTAT+AGG + tig0007116:12087-12106 None:intergenic 30.0%
TATCCACTAAAAATGACAAG+GGG + tig0007116:12113-12132 None:intergenic 30.0%
AAACAATAGAGACATACAAG+TGG - tig0007116:12183-12202 Msa1345310:CDS 30.0%
AACAATAGAGACATACAAGT+GGG - tig0007116:12184-12203 Msa1345310:CDS 30.0%
! TTTTGGATGTTTGTCATACA+TGG + tig0007116:12231-12250 None:intergenic 30.0%
!! ATTTTGACCTATCAAACAAC+AGG - tig0007116:12459-12478 Msa1345310:CDS 30.0%
TTCTAACACCACACAAAAAA+TGG + tig0007116:12541-12560 None:intergenic 30.0%
AATTATGCAAGAAATCCACA+AGG + tig0007116:12625-12644 None:intergenic 30.0%
!!! TGGAAACATTGTCATTTTCA+AGG - tig0007116:12850-12869 Msa1345310:CDS 30.0%
!! TAAATTTGTTGCGAGTGATA+AGG - tig0007116:13282-13301 Msa1345310:CDS 30.0%
ATCATTCGCAACAAATTTGT+TGG + tig0007116:13308-13327 None:intergenic 30.0%
TGTTCTAATTGTAGAAATCC+TGG - tig0007116:13391-13410 Msa1345310:CDS 30.0%
GTTCTAATTGTAGAAATCCT+GGG - tig0007116:13392-13411 Msa1345310:CDS 30.0%
GATTCATTATCAGACTCAAT+AGG + tig0007116:13490-13509 None:intergenic 30.0%
AACAAATACAAGCACTAGAA+AGG - tig0007116:13774-13793 Msa1345310:CDS 30.0%
ACAAATACAAGCACTAGAAA+GGG - tig0007116:13775-13794 Msa1345310:CDS 30.0%
! ATTTTTCATGTCCAAAGTCA+CGG + tig0007116:13945-13964 None:intergenic 30.0%
AAAACTGATCCACCATTATT+TGG + tig0007116:14056-14075 None:intergenic 30.0%
AGTGTTAGAATGTGATGATA+GGG + tig0007116:14102-14121 None:intergenic 30.0%
TGTACATAGTGATACAGATG+AGG - tig0007116:11119-11138 Msa1345310:CDS 35.0%
! ACACTGATAGTGATACAGAT+GGG - tig0007116:11169-11188 Msa1345310:intron 35.0%
GTGGAATTTAACCATGGTAA+TGG - tig0007116:11306-11325 Msa1345310:CDS 35.0%
ATGTGAAGCGTGATTCTAAT+GGG - tig0007116:11490-11509 Msa1345310:intron 35.0%
GCGTCAATTAGTATGCAATA+AGG - tig0007116:11524-11543 Msa1345310:intron 35.0%
!! AATAAGGAGGGTGTTAATGA+TGG - tig0007116:11540-11559 Msa1345310:intron 35.0%
ATCAAATGGACAAAACGCAA+AGG + tig0007116:11705-11724 None:intergenic 35.0%
TACGGTAATTAGGCATCAAA+TGG + tig0007116:11719-11738 None:intergenic 35.0%
! GTTGATAAACAAGTTGCTAG+TGG - tig0007116:11750-11769 Msa1345310:CDS 35.0%
!!! GGTTTTTTGATGGGTCAAAA+GGG - tig0007116:11810-11829 Msa1345310:intron 35.0%
AGGACTTGGGTTTATCAAAA+AGG - tig0007116:11842-11861 Msa1345310:intron 35.0%
GCAAGGGTTAAAATACAAGA+TGG - tig0007116:11891-11910 Msa1345310:intron 35.0%
! TTTGAGTTACTTCCAAGGTA+AGG - tig0007116:11929-11948 Msa1345310:CDS 35.0%
!!! GACGCCTAGAAAACATTTTT+TGG - tig0007116:12000-12019 Msa1345310:CDS 35.0%
! CTGCCCAAAAAATGTTTTCT+AGG + tig0007116:12007-12026 None:intergenic 35.0%
! AGGTTTGACTATGAGTGTTT+TGG - tig0007116:12038-12057 Msa1345310:CDS 35.0%
!!! AAACCCCTTGTCATTTTTAG+TGG - tig0007116:12107-12126 Msa1345310:CDS 35.0%
! TTTAGTGGATATAACCACCA+TGG - tig0007116:12122-12141 Msa1345310:CDS 35.0%
!! CAAAAATGGTTGTTTCTCCA+TGG + tig0007116:12142-12161 None:intergenic 35.0%
GTATGACAAACATCCAAAAG+CGG - tig0007116:12232-12251 Msa1345310:CDS 35.0%
GAGTTGTGTTTCCAAATACA+AGG - tig0007116:12294-12313 Msa1345310:CDS 35.0%
!!! TCAAACTTTTCAGGTGTACA+AGG + tig0007116:12413-12432 None:intergenic 35.0%
! CACTCAGATTCAAACTTTTC+AGG + tig0007116:12422-12441 None:intergenic 35.0%
CTGAAAAGTTTGAATCTGAG+TGG - tig0007116:12420-12439 Msa1345310:CDS 35.0%
! TTTATCGGTTGAAGAGAATG+TGG - tig0007116:12495-12514 Msa1345310:CDS 35.0%
! TTATCGGTTGAAGAGAATGT+GGG - tig0007116:12496-12515 Msa1345310:CDS 35.0%
GAAGCTTCAAAGATATTCAC+CGG - tig0007116:12749-12768 Msa1345310:CDS 35.0%
ATTTAGTCTGCAACTTGTCA+AGG + tig0007116:12916-12935 None:intergenic 35.0%
CTTCATGGCACAAATAATGT+GGG + tig0007116:12984-13003 None:intergenic 35.0%
ACTTCATGGCACAAATAATG+TGG + tig0007116:12985-13004 None:intergenic 35.0%
ATCCATGTTGTCATACTTCA+TGG + tig0007116:12999-13018 None:intergenic 35.0%
! AAAGTCTGATTTGCAAGAGA+TGG - tig0007116:13029-13048 Msa1345310:CDS 35.0%
! TAAGAGCGCAAGTGATTATA+AGG - tig0007116:13177-13196 Msa1345310:CDS 35.0%
!! ATTATAAGGAGGCTCTAGAA+GGG - tig0007116:13191-13210 Msa1345310:CDS 35.0%
TAAGATAAATAGCCGTCATG+AGG - tig0007116:13231-13250 Msa1345310:CDS 35.0%
AAGATAAATAGCCGTCATGA+GGG - tig0007116:13232-13251 Msa1345310:CDS 35.0%
ATCACTCGCAACAAATTTAC+TGG + tig0007116:13281-13300 None:intergenic 35.0%
!!! TGTTGACTTTTTACTCCTAC+GGG + tig0007116:13356-13375 None:intergenic 35.0%
!!! GTGTTGACTTTTTACTCCTA+CGG + tig0007116:13357-13376 None:intergenic 35.0%
TTAGAACAACATCTTGTCCT+TGG + tig0007116:13379-13398 None:intergenic 35.0%
TATCATTACTCATAGACAGC+TGG + tig0007116:13618-13637 None:intergenic 35.0%
!! TGCTGAAAATTGTGTTGCTT+TGG - tig0007116:13865-13884 Msa1345310:CDS 35.0%
! TTTTTCATGTCCAAAGTCAC+GGG + tig0007116:13944-13963 None:intergenic 35.0%
! CAAGTTGGAACCATAATGTT+GGG - tig0007116:13992-14011 Msa1345310:CDS 35.0%
TAACACCATACCCAACATTA+TGG + tig0007116:14005-14024 None:intergenic 35.0%
CAGTGTTAGAATGTGATGAT+AGG + tig0007116:14103-14122 None:intergenic 35.0%
ACATAGTGATACAGATGAGG+TGG - tig0007116:11122-11141 Msa1345310:CDS 40.0%
! GACACTGATAGTGATACAGA+TGG - tig0007116:11168-11187 Msa1345310:intron 40.0%
! CACTGATAGTGATACAGATG+GGG - tig0007116:11170-11189 Msa1345310:intron 40.0%
AACAACGATGTTCGTACTGA+TGG - tig0007116:11270-11289 Msa1345310:CDS 40.0%
CATTGCAAACACCATTACCA+TGG + tig0007116:11320-11339 None:intergenic 40.0%
AATGGTGTTTGCAATGAGCA+AGG - tig0007116:11324-11343 Msa1345310:CDS 40.0%
ATGGTGTTTGCAATGAGCAA+GGG - tig0007116:11325-11344 Msa1345310:CDS 40.0%
CAAGGGAAAGAGAACTTTGA+TGG - tig0007116:11342-11361 Msa1345310:intron 40.0%
GGATATGGAATTCAACACAG+AGG - tig0007116:11404-11423 Msa1345310:intron 40.0%
GATGTGAAGCGTGATTCTAA+TGG - tig0007116:11489-11508 Msa1345310:intron 40.0%
TTCGAATGGTTAGCGTAGAT+CGG + tig0007116:11587-11606 None:intergenic 40.0%
CGCAGAACAATTAGTTCGAA+TGG + tig0007116:11601-11620 None:intergenic 40.0%
ACGACTTTGTTCGTACGATT+TGG - tig0007116:11637-11656 Msa1345310:CDS 40.0%
GCTAATTCATGGTTGTGAGA+GGG + tig0007116:11681-11700 None:intergenic 40.0%
AGCTAATTCATGGTTGTGAG+AGG + tig0007116:11682-11701 None:intergenic 40.0%
AACGCAAAGGAGCTAATTCA+TGG + tig0007116:11692-11711 None:intergenic 40.0%
!!! GGGTTTTTTGATGGGTCAAA+AGG - tig0007116:11809-11828 Msa1345310:intron 40.0%
!! TTTTTGATGGGTCAAAAGGG+TGG - tig0007116:11813-11832 Msa1345310:intron 40.0%
TGATAAACCCAAGTCCTTCA+TGG + tig0007116:11839-11858 None:intergenic 40.0%
! GCTACTTTGAGTTACTTCCA+AGG - tig0007116:11924-11943 Msa1345310:CDS 40.0%
TATCAGTATCAGCCTTACCT+TGG + tig0007116:11944-11963 None:intergenic 40.0%
TTTGGGCAGATTCTACTAGT+AGG - tig0007116:12018-12037 Msa1345310:CDS 40.0%
!! AAATGGTTGTTTCTCCATGG+TGG + tig0007116:12139-12158 None:intergenic 40.0%
CAAAAGCGGTGATTACTGAT+GGG - tig0007116:12246-12265 Msa1345310:CDS 40.0%
AAAAGCGGTGATTACTGATG+GGG - tig0007116:12247-12266 Msa1345310:CDS 40.0%
AGTTTGAATCTGAGTGGAAG+AGG - tig0007116:12426-12445 Msa1345310:CDS 40.0%
GTTTGAATCTGAGTGGAAGA+GGG - tig0007116:12427-12446 Msa1345310:CDS 40.0%
TTGACCTATCAAACAACAGG+TGG - tig0007116:12462-12481 Msa1345310:CDS 40.0%
TGACCTATCAAACAACAGGT+GGG - tig0007116:12463-12482 Msa1345310:CDS 40.0%
! TTACCCACCTGTTGTTTGAT+AGG + tig0007116:12469-12488 None:intergenic 40.0%
!!! ATGCGAGACCATTTTTTGTG+TGG - tig0007116:12530-12549 Msa1345310:CDS 40.0%
AGAACGACATCAATCTGTGA+AGG - tig0007116:12557-12576 Msa1345310:CDS 40.0%
AGATGTCAAAAAGGCGATAG+AGG - tig0007116:12784-12803 Msa1345310:CDS 40.0%
CATTGAACGAGATGAAGATG+AGG - tig0007116:12826-12845 Msa1345310:CDS 40.0%
GCACAAATAATGTGGGAACA+TGG + tig0007116:12977-12996 None:intergenic 40.0%
TGCCATGAAGTATGACAACA+TGG - tig0007116:12994-13013 Msa1345310:CDS 40.0%
CTCTTGCAAATCAGACTTTC+AGG + tig0007116:13028-13047 None:intergenic 40.0%
TTCATCATTACCTTCTTGGG+AGG + tig0007116:13092-13111 None:intergenic 40.0%
AAAATGACCATGTTTCGTCG+AGG - tig0007116:13112-13131 Msa1345310:CDS 40.0%
AAATGACCATGTTTCGTCGA+GGG - tig0007116:13113-13132 Msa1345310:CDS 40.0%
ACATAGACTATTGAACGCAG+CGG + tig0007116:13146-13165 None:intergenic 40.0%
!! GATTATAAGGAGGCTCTAGA+AGG - tig0007116:13190-13209 Msa1345310:CDS 40.0%
AAATGGACATGACGAGGATA+AGG - tig0007116:13255-13274 Msa1345310:CDS 40.0%
GGAGTAAAAAGTCAACACCA+AGG - tig0007116:13359-13378 Msa1345310:CDS 40.0%
CACTGGTATTACACGTAAGA+GGG - tig0007116:13696-13715 Msa1345310:CDS 40.0%
TATGGTTCCAACTTGCACAA+TGG + tig0007116:13987-14006 None:intergenic 40.0%
! GCAAGTTGGAACCATAATGT+TGG - tig0007116:13991-14010 Msa1345310:CDS 40.0%
TGGAACCATAATGTTGGGTA+TGG - tig0007116:13997-14016 Msa1345310:CDS 40.0%
! CTATATGGGGTGCCAAATAA+TGG - tig0007116:14041-14060 Msa1345310:CDS 40.0%
TGTGATGATAGGGTAGTTGT+CGG + tig0007116:14092-14111 None:intergenic 40.0%
CAATGTTCCGAGTTATACAC+CGG - tig0007116:14160-14179 Msa1345310:CDS 40.0%
ATACTCACCGGTGTATAACT+CGG + tig0007116:14170-14189 None:intergenic 40.0%
TATGAGTGCGTTGAAGAATG+TGG - tig0007116:14270-14289 Msa1345310:CDS 40.0%
AAACATCGCAGTGACTATTG+TGG - tig0007116:14304-14323 Msa1345310:CDS 40.0%
! TGATAGTGATACAGATGGGG+TGG - tig0007116:11173-11192 Msa1345310:intron 45.0%
GACGGTGTGGAATTTAACCA+TGG - tig0007116:11300-11319 Msa1345310:CDS 45.0%
CCTTTCTTACTTCAAACCCG+TGG + tig0007116:11467-11486 None:intergenic 45.0%
! CCACGGGTTTGAAGTAAGAA+AGG - tig0007116:11464-11483 Msa1345310:CDS 45.0%
ATTTCACGGATCATCAGGCA+AGG - tig0007116:11874-11893 Msa1345310:intron 45.0%
TTTCACGGATCATCAGGCAA+GGG - tig0007116:11875-11894 Msa1345310:intron 45.0%
! CCATCAGTAATCACCGCTTT+TGG + tig0007116:12248-12267 None:intergenic 45.0%
CCAAAAGCGGTGATTACTGA+TGG - tig0007116:12245-12264 Msa1345310:CDS 45.0%
CAAAGGCGATGCCTTGTATT+TGG + tig0007116:12308-12327 None:intergenic 45.0%
AGCATTTCGATGCAAATGCC+AGG + tig0007116:12333-12352 None:intergenic 45.0%
!!! CCACAAGGCTGTTTTTGTGT+CGG + tig0007116:12610-12629 None:intergenic 45.0%
GTCAATGCAGTTGTCAGCAT+AGG + tig0007116:12716-12735 None:intergenic 45.0%
GAACGAGATGAAGATGAGGA+TGG - tig0007116:12830-12849 Msa1345310:CDS 45.0%
AATGACCATGTTTCGTCGAG+GGG - tig0007116:13114-13133 Msa1345310:CDS 45.0%
GAGCGCAAGTGATTATAAGG+AGG - tig0007116:13180-13199 Msa1345310:CDS 45.0%
AATAGCCGTCATGAGGGAAA+TGG - tig0007116:13238-13257 Msa1345310:CDS 45.0%
CATGTCCATTTCCCTCATGA+CGG + tig0007116:13246-13265 None:intergenic 45.0%
TGTTGCGAATGATCCACTGA+TGG - tig0007116:13315-13334 Msa1345310:CDS 45.0%
CCTTTGACATTCACCATCAG+TGG + tig0007116:13331-13350 None:intergenic 45.0%
! CCACTGATGGTGAATGTCAA+AGG - tig0007116:13328-13347 Msa1345310:CDS 45.0%
GAAATCCTGGGCACACAATA+CGG - tig0007116:13404-13423 Msa1345310:CDS 45.0%
!! TGTCCAGAACTTGCTGGAAT+TGG - tig0007116:13430-13449 Msa1345310:CDS 45.0%
ACACCAATTCCAGCAAGTTC+TGG + tig0007116:13436-13455 None:intergenic 45.0%
GTGTCGTGTCTGAAGATCAA+GGG - tig0007116:13452-13471 Msa1345310:CDS 45.0%
TGTCGTGTCTGAAGATCAAG+GGG - tig0007116:13453-13472 Msa1345310:CDS 45.0%
CATTTGACACTGCTACAGAG+TGG + tig0007116:13654-13673 None:intergenic 45.0%
CACTCTGTAGCAGTGTCAAA+TGG - tig0007116:13652-13671 Msa1345310:CDS 45.0%
TCTGTAGCAGTGTCAAATGG+TGG - tig0007116:13655-13674 Msa1345310:CDS 45.0%
! CGAGATGTTGAGAAAAGCAC+TGG - tig0007116:13679-13698 Msa1345310:CDS 45.0%
! GCACTGGTATTACACGTAAG+AGG - tig0007116:13695-13714 Msa1345310:CDS 45.0%
AAGACAGCCATTGTGCAAGT+TGG - tig0007116:13977-13996 Msa1345310:CDS 45.0%
! TATGGGGTGCCAAATAATGG+TGG - tig0007116:14044-14063 Msa1345310:CDS 45.0%
ATGATAGGGTAGTTGTCGGA+GGG + tig0007116:14088-14107 None:intergenic 45.0%
TCTAACACTGCTCTGCATAC+TGG - tig0007116:14113-14132 Msa1345310:CDS 45.0%
ACTGGAACACACAATGTTCC+TGG - tig0007116:14131-14150 Msa1345310:CDS 45.0%
! CGTACTGATGGAGATGCAGA+CGG - tig0007116:11282-11301 Msa1345310:CDS 50.0%
GCTAGTGGTCTACATTCGCA+AGG - tig0007116:11765-11784 Msa1345310:CDS 50.0%
CCGACACAAAAACAGCCTTG+TGG - tig0007116:12607-12626 Msa1345310:CDS 50.0%
! TGTAGACGCTTTGAACGCAG+AGG - tig0007116:12947-12966 Msa1345310:CDS 50.0%
TCGTCATTTGCCTCCCAAGA+AGG - tig0007116:13079-13098 Msa1345310:CDS 50.0%
TGAGGGAAATGGACATGACG+AGG - tig0007116:13249-13268 Msa1345310:CDS 50.0%
!! TGAATGTCAAAGGTGCCCGT+AGG - tig0007116:13338-13357 Msa1345310:CDS 50.0%
! GGTGTCGTGTCTGAAGATCA+AGG - tig0007116:13451-13470 Msa1345310:CDS 50.0%
GTCTGAAGATCAAGGGGAGA+AGG - tig0007116:13459-13478 Msa1345310:CDS 50.0%
AATGGTGTGACCCGTGACTT+TGG - tig0007116:13931-13950 Msa1345310:CDS 50.0%
GTAGTTGTCGGAGGGAATTG+TGG + tig0007116:14080-14099 None:intergenic 50.0%
GATGATAGGGTAGTTGTCGG+AGG + tig0007116:14089-14108 None:intergenic 50.0%
CTCGGAACATTGTGTGTTCC+AGG + tig0007116:14152-14171 None:intergenic 50.0%
TGATGGAGATGCAGACGGTG+TGG - tig0007116:11287-11306 Msa1345310:CDS 55.0%
GGTCAAAAGGGTGGCCATGA+AGG - tig0007116:11822-11841 Msa1345310:intron 55.0%
AAGGGTGGCCATGAAGGACT+TGG - tig0007116:11828-11847 Msa1345310:intron 55.0%
AGGGTGGCCATGAAGGACTT+GGG - tig0007116:11829-11848 Msa1345310:intron 55.0%
GATGCAAATGCCAGGCGCAA+AGG + tig0007116:12325-12344 None:intergenic 55.0%
AAGTGCCCCTCGACGAAACA+TGG + tig0007116:13122-13141 None:intergenic 55.0%
CACGTCCGTATTGTGTGCCC+AGG + tig0007116:13412-13431 None:intergenic 60.0%
CGGACGTGTCCAGAACTTGC+TGG - tig0007116:13424-13443 Msa1345310:CDS 60.0%
!! CAAGGCATCGCCTTTGCGCC+TGG - tig0007116:12312-12331 Msa1345310:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0007116 gene 11084 14339 11084 ID=Msa1345310;Name=Msa1345310
tig0007116 mRNA 11084 14339 11084 ID=Msa1345310-mRNA-1;Parent=Msa1345310;Name=Msa1345310-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0|0|0.2|1|1|5|0|968
tig0007116 exon 11901 14339 11901 ID=Msa1345310-mRNA-1:exon:4959;Parent=Msa1345310-mRNA-1
tig0007116 exon 11583 11804 11583 ID=Msa1345310-mRNA-1:exon:4958;Parent=Msa1345310-mRNA-1
tig0007116 exon 11429 11492 11429 ID=Msa1345310-mRNA-1:exon:4957;Parent=Msa1345310-mRNA-1
tig0007116 exon 11242 11351 11242 ID=Msa1345310-mRNA-1:exon:4956;Parent=Msa1345310-mRNA-1
tig0007116 exon 11084 11155 11084 ID=Msa1345310-mRNA-1:exon:4955;Parent=Msa1345310-mRNA-1
tig0007116 CDS 11901 14339 11901 ID=Msa1345310-mRNA-1:cds;Parent=Msa1345310-mRNA-1
tig0007116 CDS 11583 11804 11583 ID=Msa1345310-mRNA-1:cds;Parent=Msa1345310-mRNA-1
tig0007116 CDS 11429 11492 11429 ID=Msa1345310-mRNA-1:cds;Parent=Msa1345310-mRNA-1
tig0007116 CDS 11242 11351 11242 ID=Msa1345310-mRNA-1:cds;Parent=Msa1345310-mRNA-1
tig0007116 CDS 11084 11155 11084 ID=Msa1345310-mRNA-1:cds;Parent=Msa1345310-mRNA-1
Gene Sequence

>Msa1345310

ATGTCTACGTATAGTGAAGCAACATTGCCTTTTGATGTACATAGTGATACAGATGAGGTGGAATTTAATAGTGTTAATGATATTGACACTGATAGTGATACAGATGGGGTGGAATTTAATAGTGTTAATGATGTTGACACTGATAGTGACACTGATAGTGATACTGAAGATATAAAAGTCAATAGCAACAACGATGTTCGTACTGATGGAGATGCAGACGGTGTGGAATTTAACCATGGTAATGGTGTTTGCAATGAGCAAGGGAAAGAGAACTTTGATGGCTACAAATGCATCAGTGATGTGACAGATGATGATATAAGGGATATGGAATTCAACACAGAGGATGAAGCTATAAAGTTTTATGTATTGTTTGCAAAATACCACGGGTTTGAAGTAAGAAAGGATGATGTGAAGCGTGATTCTAATGGGAACATTATTATGCGTCAATTAGTATGCAATAAGGAGGGTGTTAATGATGGAAAAAATAAGAAGAATGGCAGCCGATCTACGCTAACCATTCGAACTAATTGTTCTGCGAAACTTCGAGTAGTCTACGACTTTGTTCGTACGATTTGGAGAGTTTCTATTTTCAAACCCTCTCACAACCATGAATTAGCTCCTTTGCGTTTTGTCCATTTGATGCCTAATTACCGTAAATTGAGTGAAGTTGATAAACAAGTTGCTAGTGGTCTACATTCGCAAGGTGTAAGAACATGTCATATCTTGGGTTTTTTGATGGGTCAAAAGGGTGGCCATGAAGGACTTGGGTTTATCAAAAAGGACTTATATAATTTCACGGATCATCAGGCAAGGGTTAAAATACAAGATGGGGATACTTTTGCTACTTTGAGTTACTTCCAAGGTAAGGCTGATACTGATACATCATTCTTTTCAGAGTTTACATTTACAGATGATGGACGCCTAGAAAACATTTTTTGGGCAGATTCTACTAGTAGGTTTGACTATGAGTGTTTTGGCGATGTTGTTGCATTTGACACCACCTATAGAAAGAACAAGTATGGAAAACCCCTTGTCATTTTTAGTGGATATAACCACCATGGAGAAACAACCATTTTTGCTTGTGCTTTAATTTGTGATGAAACAATAGAGACATACAAGTGGGTTCTAAATTTGTTTTCAAAGGCCATGTATGACAAACATCCAAAAGCGGTGATTACTGATGGGGATAAATCAATGAGAAAAGCTATCAGAGTTGTGTTTCCAAATACAAGGCATCGCCTTTGCGCCTGGCATTTGCATCGAAATGCTGTTCGACATGTGAAGAATTCAAAATTTTTAGAAGAGTTCAAGTCACTGATGTATTCTCCTTGTACACCTGAAAAGTTTGAATCTGAGTGGAAGAGGGTTGTTGAAAATTTTGACCTATCAAACAACAGGTGGGTAAAAAAAATTTATCGGTTGAAGAGAATGTGGGCAAGTGCATACATGCGAGACCATTTTTTGTGTGGTGTTAGAACGACATCAATCTGTGAAGGAGTGAATTCATTTATCAAGAAGTATGTCCGACACAAAAACAGCCTTGTGGATTTCTTGCATAATTTTGAAAGAGCTCTCAAAGAATACAGACATAATGAATTATTGTCTGATTTCAAGTCGTTCTATTATCATCCTATGCTGACAACTGCATTGACTACTTTTGAGTCTGAAGCTTCAAAGATATTCACCGGTAAAAAGTTTAAAGATGTCAAAAAGGCGATAGAGGATGTTGTTGCATTATCAGTCATTGAACGAGATGAAGATGAGGATGGAAACATTGTCATTTTCAAGGTTAATGTATTTGGCAAAAAAGATGTAGCTTTTTCGGTATACCTTGACAAGTTGCAGACTAAATTTTTATGTGATTGTAGACGCTTTGAACGCAGAGGACTGCCATGTTCCCACATTATTTGTGCCATGAAGTATGACAACATGGATGTGTTTCCTGAAAGTCTGATTTGCAAGAGATGGACAAAGACTGCAAAGAGTGATCACATCTCGTCATTTGCCTCCCAAGAAGGTAATGATGAAAAAATGACCATGTTTCGTCGAGGGGCACTTGCCGCTGCGTTCAATAGTCTATGTGATGTTTCGTGTAAGAGCGCAAGTGATTATAAGGAGGCTCTAGAAGGGATTTATAGTCTGTGTAATAAGATAAATAGCCGTCATGAGGGAAATGGACATGACGAGGATAAGGCCAGTAAATTTGTTGCGAGTGATAAGGCCAACAAATTTGTTGCGAATGATCCACTGATGGTGAATGTCAAAGGTGCCCGTAGGAGTAAAAAGTCAACACCAAGGACAAGATGTTGTTCTAATTGTAGAAATCCTGGGCACACAATACGGACGTGTCCAGAACTTGCTGGAATTGGTGTCGTGTCTGAAGATCAAGGGGAGAAGGCTTCACCTATTGAGTCTGATAATGAATCAAAAAGTTTAAGTCTGTCTATGAGTAATGATATGAATGATGACATCCACTCTGTAGCAGTGTCAAATGGTGGACGAGATGTTGAGAAAAGCACTGGTATTACACGTAAGAGGGAATGTGACAATGTTGCATTTTCAACACAAGACTCTGTCAACAAAAGAAAAACAAAAACAAATACAAGCACTAGAAAGGGTGATGCTTGTGTGACTCCAAATATCACAATCAACATTGATAATAATGGTGTGACCCGTGACTTTGGACATGAAAAATCTACTCTCAAACAAGACAGCCATTGTGCAATTTTTCCACAATTCCCTCCGACAACTACCCTATCATCACATTCTAACACTGCTCTGCATACTGGAACACACAATGTTCCTGGAACACACAATGTTCCGAGTTATACACCGTTTATGAGTGCGTTGAAGAATGTGGAGCTGTTAGCAAAACATCGCAGTGACTATTGTGGACCAAAACATTGA
Protein sequence

>Msa1345310

MSTYSEATLPFDVHSDTDEVEFNSVNDIDTDSDTDGVEFNSVNDVDTDSDTDSDTEDIKVNSNNDVRTDGDADGVEFNHGNGVCNEQGKENFDGYKCISDVTDDDIRDMEFNTEDEAIKFYVLFAKYHGFEVRKDDVKRDSNGNIIMRQLVCNKEGVNDGKNKKNGSRSTLTIRTNCSAKLRVVYDFVRTIWRVSIFKPSHNHELAPLRFVHLMPNYRKLSEVDKQVASGLHSQGVRTCHILGFLMGQKGGHEGLGFIKKDLYNFTDHQARVKIQDGDTFATLSYFQGKADTDTSFFSEFTFTDDGRLENIFWADSTSRFDYECFGDVVAFDTTYRKNKYGKPLVIFSGYNHHGETTIFACALICDETIETYKWVLNLFSKAMYDKHPKAVITDGDKSMRKAIRVVFPNTRHRLCAWHLHRNAVRHVKNSKFLEEFKSLMYSPCTPEKFESEWKRVVENFDLSNNRWVKKIYRLKRMWASAYMRDHFLCGVRTTSICEGVNSFIKKYVRHKNSLVDFLHNFERALKEYRHNELLSDFKSFYYHPMLTTALTTFESEASKIFTGKKFKDVKKAIEDVVALSVIERDEDEDGNIVIFKVNVFGKKDVAFSVYLDKLQTKFLCDCRRFERRGLPCSHIICAMKYDNMDVFPESLICKRWTKTAKSDHISSFASQEGNDEKMTMFRRGALAAAFNSLCDVSCKSASDYKEALEGIYSLCNKINSRHEGNGHDEDKASKFVASDKANKFVANDPLMVNVKGARRSKKSTPRTRCCSNCRNPGHTIRTCPELAGIGVVSEDQGEKASPIESDNESKSLSLSMSNDMNDDIHSVAVSNGGRDVEKSTGITRKRECDNVAFSTQDSVNKRKTKTNTSTRKGDACVTPNITINIDNNGVTRDFGHEKSTLKQDSHCAIFPQFPPTTTLSSHSNTALHTGTHNVPGTHNVPSYTPFMSALKNVELLAKHRSDYCGPKH*