AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0759280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0759280 MtrunA17_Chr2g0287651 42.960 696 378 6 93 783 63 744 0.0 586
Msa0759280 MtrunA17_Chr1g0203051 34.564 596 367 6 68 648 34 621 3.95e-117 373
Msa0759280 MtrunA17_Chr1g0202341 32.709 587 372 10 104 676 50 627 5.53e-97 321
Msa0759280 MtrunA17_Chr5g0448761 32.632 570 363 8 109 663 64 627 4.81e-94 316
Msa0759280 MtrunA17_Chr2g0324011 29.181 586 397 6 109 682 14 593 7.15e-86 288
Msa0759280 MtrunA17_Chr2g0296781 29.204 678 433 15 109 764 1 653 2.54e-84 288
Msa0759280 MtrunA17_Chr8g0363841 28.081 641 424 11 101 716 9 637 4.58e-84 285
Msa0759280 MtrunA17_Chr5g0441581 28.346 635 408 12 109 724 36 642 1.10e-82 282
Msa0759280 MtrunA17_Chr1g0198761 31.196 577 366 11 111 665 53 620 1.20e-80 278
Msa0759280 MtrunA17_Chr3g0082121 30.099 608 396 12 108 704 46 635 8.85e-80 274
Msa0759280 MtrunA17_Chr6g0477261 34.813 428 264 6 245 661 22 445 2.45e-78 269
Msa0759280 MtrunA17_Chr1g0203721 27.885 624 429 11 111 724 69 681 1.21e-76 270
Msa0759280 MtrunA17_Chr1g0207551 30.838 561 371 9 109 661 38 589 3.10e-75 264
Msa0759280 MtrunA17_Chr6g0458391 28.042 567 364 11 109 661 229 765 1.38e-72 256
Msa0759280 MtrunA17_Chr3g0080351 27.885 624 383 13 100 699 71 651 9.80e-72 255
Msa0759280 MtrunA17_Chr1g0196541 30.594 572 362 15 109 661 1 556 2.37e-70 249
Msa0759280 MtrunA17_Chr2g0329031 26.094 617 409 11 92 694 1 584 1.91e-69 246
Msa0759280 MtrunA17_Chr5g0440451 27.404 624 405 16 109 709 47 645 3.66e-65 237
Msa0759280 MtrunA17_Chr3g0118611 29.862 509 325 11 211 709 56 542 1.81e-64 233
Msa0759280 MtrunA17_Chr5g0448511 30.653 398 266 5 271 662 5 398 1.84e-64 228
Msa0759280 MtrunA17_Chr2g0276841 27.360 625 424 14 111 721 76 684 1.04e-63 229
Msa0759280 MtrunA17_Chr5g0431001 32.151 423 261 8 111 512 67 484 3.05e-63 223
Msa0759280 MtrunA17_Chr2g0290391 26.596 658 417 16 109 730 55 682 2.00e-61 222
Msa0759280 MtrunA17_Chr2g0291581 27.592 569 374 13 109 661 75 621 4.10e-60 218
Msa0759280 MtrunA17_Chr2g0287061 27.723 606 387 14 110 709 188 748 1.61e-59 219
Msa0759280 MtrunA17_Chr2g0291591 30.453 486 312 10 106 580 65 535 1.63e-58 211
Msa0759280 MtrunA17_Chr2g0294841 28.196 571 379 14 111 662 51 609 1.33e-57 213
Msa0759280 MtrunA17_Chr1g0164591 32.459 305 192 3 192 491 12 307 2.22e-50 181
Msa0759280 MtrunA17_Chr2g0308841 33.051 236 156 1 436 671 4 237 1.33e-38 145
Msa0759280 MtrunA17_Chr7g0258861 31.579 285 176 5 109 382 14 290 1.02e-37 144
Msa0759280 MtrunA17_Chr1g0149131 34.545 110 67 1 383 487 1 110 2.94e-16 76.3
Msa0759280 MtrunA17_Chr8g0340801 33.945 109 70 1 213 321 1 107 3.81e-14 69.7
Msa0759280 MtrunA17_Chr6g0451441 25.600 250 170 6 31 269 2 246 5.91e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0759280 AT4G38180.1 31.424 646 420 12 33 665 3 638 8.33e-94 315
Msa0759280 AT4G15090.1 29.701 670 441 14 57 711 2 656 1.87e-85 293
Msa0759280 AT4G15090.5 29.701 670 441 14 57 711 2 656 1.87e-85 293
Msa0759280 AT4G15090.3 29.701 670 441 14 57 711 2 656 1.87e-85 293
Msa0759280 AT4G15090.2 29.701 670 441 14 57 711 2 656 1.87e-85 293
Msa0759280 AT4G15090.4 29.701 670 441 14 57 711 2 656 1.87e-85 293
Msa0759280 AT3G22170.2 26.744 688 440 14 56 720 30 676 1.94e-72 257
Msa0759280 AT3G22170.1 26.744 688 440 14 56 720 30 676 1.94e-72 257
Msa0759280 AT2G27110.2 26.147 654 416 14 63 703 14 613 3.72e-67 242
Msa0759280 AT2G27110.1 26.147 654 416 14 63 703 14 613 3.72e-67 242
Msa0759280 AT5G28530.1 28.423 577 381 11 111 667 61 625 1.66e-64 231
Msa0759280 AT3G06250.2 27.107 605 390 11 109 709 194 751 3.55e-64 232
Msa0759280 AT3G06250.1 27.107 605 390 11 109 709 194 751 3.55e-64 232
Msa0759280 AT3G06250.3 27.107 605 390 11 109 709 194 751 3.55e-64 232
Msa0759280 AT1G10240.2 27.244 635 412 13 111 721 54 662 1.26e-63 229
Msa0759280 AT1G10240.1 27.244 635 412 13 111 721 54 662 1.26e-63 229
Msa0759280 AT5G28530.3 28.076 577 379 11 111 667 61 621 3.41e-63 228
Msa0759280 AT5G28530.2 28.076 577 379 11 111 667 61 621 3.41e-63 228
Msa0759280 AT1G76320.1 27.051 573 375 13 109 664 1 547 1.21e-62 227
Msa0759280 AT1G76320.4 27.051 573 375 13 109 664 1 547 1.21e-62 227
Msa0759280 AT1G76320.2 27.051 573 375 13 109 664 1 547 1.46e-62 227
Msa0759280 AT1G76320.3 27.051 573 375 13 109 664 1 547 1.80e-62 225
Msa0759280 AT2G27110.3 30.324 432 281 7 278 703 51 468 3.50e-62 225
Msa0759280 AT4G19990.3 27.903 577 356 13 110 665 26 563 6.43e-62 225
Msa0759280 AT4G19990.4 27.903 577 356 13 110 665 26 563 6.43e-62 225
Msa0759280 AT4G19990.6 27.556 577 358 12 110 665 26 563 7.86e-62 225
Msa0759280 AT4G19990.2 27.556 577 358 12 110 665 26 563 7.86e-62 225
Msa0759280 AT4G19990.7 27.451 612 375 14 83 665 6 577 8.10e-62 225
Msa0759280 AT4G19990.5 27.903 577 356 13 110 665 26 563 8.93e-62 225
Msa0759280 AT1G52520.1 27.604 576 378 13 109 666 89 643 9.58e-62 224
Msa0759280 AT1G80010.1 28.155 618 387 20 84 670 79 670 1.03e-61 224
Msa0759280 AT5G18960.2 26.733 606 394 13 109 709 215 775 3.58e-60 221
Msa0759280 AT5G18960.1 26.733 606 394 13 109 709 215 775 3.58e-60 221
Msa0759280 AT4G19990.1 29.825 399 272 4 273 665 110 506 3.90e-59 216
Msa0759280 AT2G32250.2 26.904 617 365 14 109 701 42 596 2.01e-56 210
Msa0759280 AT2G32250.4 26.904 617 365 14 109 701 42 596 2.01e-56 210
Msa0759280 AT2G32250.8 26.742 617 366 14 109 701 1 555 2.16e-56 209
Msa0759280 AT2G32250.6 26.904 617 365 14 109 701 42 596 2.58e-56 210
Msa0759280 AT2G32250.1 26.904 617 365 14 109 701 42 596 2.58e-56 210
Msa0759280 AT2G32250.3 26.904 617 365 14 109 701 42 596 2.58e-56 210
Msa0759280 AT2G32250.7 26.742 617 366 14 109 701 1 555 2.82e-56 209
Msa0759280 AT2G32250.5 26.904 617 365 14 109 701 42 596 3.30e-56 209
Msa0759280 AT4G38170.1 28.337 427 277 9 310 720 45 458 5.74e-55 201
Msa0759280 AT5G28530.4 25.397 567 348 14 111 667 61 562 1.13e-43 169
Msa0759280 AT5G28530.5 25.044 567 346 14 111 667 61 558 1.30e-42 166

Find 173 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACTGATCCACCATTATT+TGG 0.155571 5_2:+14834133 None:intergenic
CAAAGGCGATGCCTTGTATT+TGG 0.159430 5_2:+14835879 None:intergenic
TCAATGTTGATTGTGATATT+TGG 0.171428 5_2:+14834370 None:intergenic
CTCTTGCAAATCAGACTTTC+AGG 0.192125 5_2:+14835159 None:intergenic
TCACTCAATTTACGGTAATT+AGG 0.235603 5_2:+14836458 None:intergenic
CCCATAGTTTCTCAATGTTT+TGG 0.242135 5_2:+14833858 None:intergenic
TATCAACTTCACTCAATTTA+CGG 0.276736 5_2:+14836450 None:intergenic
ATCACTCGCAACAAATTTAC+TGG 0.280329 5_2:+14834906 None:intergenic
CCATCAGTAATCACCGCTTT+TGG 0.302214 5_2:+14835939 None:intergenic
TCCATACTTGTTCTTTCTAT+AGG 0.305560 5_2:+14836100 None:intergenic
TAACACCATACCCAACATTA+TGG 0.313783 5_2:+14834182 None:intergenic
AGGACTTGGGTTTATCAAAA+AGG 0.326599 5_2:-14836342 Msa0759280:CDS
CAGTGTTAGAATGTGATGAT+AGG 0.338987 5_2:+14834086 None:intergenic
TGATAAACCCAAGTCCTTCA+TGG 0.346028 5_2:+14836348 None:intergenic
AGATGATGATATAAGGGATA+TGG 0.347376 5_2:-14836795 Msa0759280:CDS
ACCTATAGAAAGAACAAGTA+TGG 0.349144 5_2:-14836101 Msa0759280:CDS
ACGACTTTGTTCGTACGATT+TGG 0.356788 5_2:-14836547 Msa0759280:CDS
ACACCAATTCCAGCAAGTTC+TGG 0.360337 5_2:+14834751 None:intergenic
ATGTGACAGATGATGATATA+AGG 0.367162 5_2:-14836802 Msa0759280:CDS
GAGTTTACATTTACAGATGA+TGG 0.367609 5_2:-14836206 Msa0759280:CDS
CTCGGAACATTGTGTGTTCC+AGG 0.370631 5_2:+14834037 None:intergenic
TAAGAGCGCAAGTGATTATA+AGG 0.371814 5_2:-14835007 Msa0759280:CDS
AGGTTTGACTATGAGTGTTT+TGG 0.377109 5_2:-14836146 Msa0759280:CDS
TTACCCACCTGTTGTTTGAT+AGG 0.378005 5_2:+14835718 None:intergenic
TGTAAGAACATGTCATATCT+TGG 0.387474 5_2:-14836396 Msa0759280:CDS
TGTTCTAATTGTAGAAATCC+TGG 0.392101 5_2:-14834793 Msa0759280:CDS
CACTATGGGGTGCCAAATAA+TGG 0.393131 5_2:-14834145 Msa0759280:CDS
ATCCATGTTGTCATACTTCA+TGG 0.394339 5_2:+14835188 None:intergenic
ACTGGAACACACAATGTTCC+TGG 0.398163 5_2:-14834055 Msa0759280:CDS
ATCATTCGCAACAAATTTGT+TGG 0.403036 5_2:+14834879 None:intergenic
TCTAACACTGCTCTGCATAC+TGG 0.407068 5_2:-14834073 Msa0759280:CDS
ATATAATTTCACGGATCATC+AGG 0.409639 5_2:-14836315 Msa0759280:CDS
TTTGGGCAGATTCTACTAGT+AGG 0.409763 5_2:-14836166 Msa0759280:CDS
GATTATAAGGAGGCTCTAGA+AGG 0.413898 5_2:-14834994 Msa0759280:CDS
GATTCATTATCAGACTCAAT+AGG 0.414121 5_2:+14834697 None:intergenic
GCACTGGTATTACACGTAAG+AGG 0.418801 5_2:-14834489 Msa0759280:CDS
CAAGGCATCGCCTTTGCGCC+TGG 0.420887 5_2:-14835872 Msa0759280:CDS
AACAAATACAAGCACTAGAA+AGG 0.422563 5_2:-14834410 Msa0759280:CDS
TGTCCAGAACTTGCTGGAAT+TGG 0.422787 5_2:-14834754 Msa0759280:CDS
AAAGTTTAAAGATGTCAAAA+AGG 0.436388 5_2:-14835409 Msa0759280:CDS
TACGGTAATTAGGCATCAAA+TGG 0.442752 5_2:+14836468 None:intergenic
CGCAGAACAATTAGTTCGAA+TGG 0.445789 5_2:+14836586 None:intergenic
AAGGGTGGCCATGAAGGACT+TGG 0.449428 5_2:-14836356 Msa0759280:CDS
TAAATTTGTTGCGAGTGATA+AGG 0.460501 5_2:-14834902 Msa0759280:CDS
AAATGGACATGACGAGGATA+AGG 0.462491 5_2:-14834929 Msa0759280:CDS
ATACTTGTTCTTTCTATAGG+TGG 0.464692 5_2:+14836103 None:intergenic
GTAAGAACATGTCATATCTT+GGG 0.464989 5_2:-14836395 Msa0759280:CDS
GCGTCAATTAGTATGCAATA+AGG 0.467578 5_2:-14836660 Msa0759280:CDS
AACGCAAAGGAGCTAATTCA+TGG 0.469712 5_2:+14836495 None:intergenic
AGTGTTAGAATGTGATGATA+GGG 0.470693 5_2:+14834087 None:intergenic
TTCGAATGGTTAGCGTAGAT+CGG 0.471186 5_2:+14836600 None:intergenic
AATAGCCGTCATGAGGGAAA+TGG 0.477370 5_2:-14834946 Msa0759280:CDS
CAAGGGAAAGAGAACTTTGA+TGG 0.478759 5_2:-14836842 Msa0759280:CDS
CGGACGTGTCCAGAACTTGC+TGG 0.483560 5_2:-14834760 Msa0759280:CDS
AAAGGACTTATATAATTTCA+CGG 0.493961 5_2:-14836324 Msa0759280:CDS
GAAATCCTGGGCACACAATA+CGG 0.497533 5_2:-14834780 Msa0759280:CDS
CACTCTGTAGCAGTGTCAAA+TGG 0.499398 5_2:-14834532 Msa0759280:CDS
CCAAAAGCGGTGATTACTGA+TGG 0.502215 5_2:-14835939 Msa0759280:CDS
ATGTGAAGCGTGATTCTAAT+GGG 0.504223 5_2:-14836694 Msa0759280:CDS
AATGGTGTGACCCGTGACTT+TGG 0.505092 5_2:-14834253 Msa0759280:CDS
GCAAGGGTTAAAATACAAGA+TGG 0.508614 5_2:-14836293 Msa0759280:CDS
GTATCACTATGTACATCAAA+AGG 0.509968 5_2:+14837073 None:intergenic
GGTCAAAAGGGTGGCCATGA+AGG 0.510809 5_2:-14836362 Msa0759280:CDS
TTTGAGTTACTTCCAAGGTA+AGG 0.511685 5_2:-14836255 Msa0759280:CDS
CCACGGGTTTGAAGTAAGAA+AGG 0.512169 5_2:-14836720 Msa0759280:CDS
TTAGAACAACATCTTGTCCT+TGG 0.513299 5_2:+14834808 None:intergenic
TGTGATGATAGGGTAGTTGT+CGG 0.517812 5_2:+14834097 None:intergenic
AGTTTGAATCTGAGTGGAAG+AGG 0.520947 5_2:-14835758 Msa0759280:CDS
ATTTAGTCTGCAACTTGTCA+AGG 0.524883 5_2:+14835271 None:intergenic
CAAGGGTTAAAATACAAGAT+GGG 0.527779 5_2:-14836292 Msa0759280:CDS
AACAACGATGTTCGTACTGA+TGG 0.529175 5_2:-14836914 Msa0759280:CDS
AAAGTCTGATTTGCAAGAGA+TGG 0.531904 5_2:-14835155 Msa0759280:CDS
GATGTGAAGCGTGATTCTAA+TGG 0.532287 5_2:-14836695 Msa0759280:CDS
GTTCTAATTGTAGAAATCCT+GGG 0.533951 5_2:-14834792 Msa0759280:CDS
AAGTGCCCCTCGACGAAACA+TGG 0.534361 5_2:+14835065 None:intergenic
CACGTCCGTATTGTGTGCCC+AGG 0.534840 5_2:+14834775 None:intergenic
ACAAATACAAGCACTAGAAA+GGG 0.535750 5_2:-14834409 Msa0759280:CDS
AGCATTTCGATGCAAATGCC+AGG 0.536176 5_2:+14835854 None:intergenic
GTAGTTGTCGGAGGGAATTG+TGG 0.539742 5_2:+14834109 None:intergenic
TTTATCGGTTGAAGAGAATG+TGG 0.541414 5_2:-14835689 Msa0759280:CDS
GAGTTGTGTTTCCAAATACA+AGG 0.541485 5_2:-14835890 Msa0759280:CDS
AATAAGGAGGGTGTTAATGA+TGG 0.543414 5_2:-14836644 Msa0759280:CDS
AAGACAGCCATTGTGCAAGT+TGG 0.545484 5_2:-14834207 Msa0759280:CDS
AGCTAATTCATGGTTGTGAG+AGG 0.545517 5_2:+14836505 None:intergenic
TATCAGTATCAGCCTTACCT+TGG 0.546983 5_2:+14836243 None:intergenic
ACTTCATGGCACAAATAATG+TGG 0.547507 5_2:+14835202 None:intergenic
CAAAAGCGGTGATTACTGAT+GGG 0.550752 5_2:-14835938 Msa0759280:CDS
TTGACCTATCAAACAACAGG+TGG 0.553778 5_2:-14835722 Msa0759280:CDS
GATGCAAATGCCAGGCGCAA+AGG 0.554082 5_2:+14835862 None:intergenic
TCGTCATTTGCCTCCCAAGA+AGG 0.555407 5_2:-14835105 Msa0759280:CDS
GTTTGAATCTGAGTGGAAGA+GGG 0.557830 5_2:-14835757 Msa0759280:CDS
CACTGGTATTACACGTAAGA+GGG 0.559146 5_2:-14834488 Msa0759280:CDS
GGTGTCGTGTCTGAAGATCA+AGG 0.560464 5_2:-14834733 Msa0759280:CDS
CGAGATGTTGAGAAAAGCAC+TGG 0.563759 5_2:-14834505 Msa0759280:CDS
TTCATCATTACCTTCTTGGG+AGG 0.565482 5_2:+14835095 None:intergenic
GTCTGAAGATCAAGGGGAGA+AGG 0.567087 5_2:-14834725 Msa0759280:CDS
TATTGTTTGCAAAATACCAC+GGG 0.573778 5_2:-14836736 Msa0759280:CDS
CCTTTGACATTCACCATCAG+TGG 0.574175 5_2:+14834856 None:intergenic
GTGGAATTTAACCATGGTAA+TGG 0.576772 5_2:-14836878 Msa0759280:CDS
GCACAAATAATGTGGGAACA+TGG 0.578248 5_2:+14835210 None:intergenic
TGTGACAGATGATGATATAA+GGG 0.581249 5_2:-14836801 Msa0759280:CDS
AAATGACCATGTTTCGTCGA+GGG 0.581274 5_2:-14835071 Msa0759280:CDS
GTGTCGTGTCTGAAGATCAA+GGG 0.581492 5_2:-14834732 Msa0759280:CDS
GCTAATTCATGGTTGTGAGA+GGG 0.581523 5_2:+14836506 None:intergenic
TCAATTAGTATGCAATAAGG+AGG 0.584633 5_2:-14836657 Msa0759280:CDS
AATGACCATGTTTCGTCGAG+GGG 0.585541 5_2:-14835070 Msa0759280:CDS
GCAAGTTGGAACCATAATGT+TGG 0.585606 5_2:-14834193 Msa0759280:CDS
TAAGATAAATAGCCGTCATG+AGG 0.585792 5_2:-14834953 Msa0759280:CDS
AGGGTGGCCATGAAGGACTT+GGG 0.586883 5_2:-14836355 Msa0759280:CDS
GAAGCTTCAAAGATATTCAC+CGG 0.587720 5_2:-14835435 Msa0759280:CDS
AAGATAAATAGCCGTCATGA+GGG 0.587755 5_2:-14834952 Msa0759280:CDS
ATACTCACCGGTGTATAACT+CGG 0.588936 5_2:+14834019 None:intergenic
ATGATAGGGTAGTTGTCGGA+GGG 0.591320 5_2:+14834101 None:intergenic
ATTTCACGGATCATCAGGCA+AGG 0.592596 5_2:-14836310 Msa0759280:CDS
TGGAACCATAATGTTGGGTA+TGG 0.594448 5_2:-14834187 Msa0759280:CDS
CTTCATGGCACAAATAATGT+GGG 0.594948 5_2:+14835203 None:intergenic
TTATCGGTTGAAGAGAATGT+GGG 0.595180 5_2:-14835688 Msa0759280:CDS
TATGAGTGCGTTGAAGAATG+TGG 0.597255 5_2:-14833916 Msa0759280:CDS
GAACGAGATGAAGATGAGGA+TGG 0.598765 5_2:-14835354 Msa0759280:CDS
GTATTGTTTGCAAAATACCA+CGG 0.599210 5_2:-14836737 Msa0759280:CDS
CAAGTTGGAACCATAATGTT+GGG 0.599464 5_2:-14834192 Msa0759280:CDS
GTCAATGCAGTTGTCAGCAT+AGG 0.607551 5_2:+14835471 None:intergenic
CTGAAAAGTTTGAATCTGAG+TGG 0.609008 5_2:-14835764 Msa0759280:CDS
AAGGGTTAAAATACAAGATG+GGG 0.610552 5_2:-14836291 Msa0759280:CDS
AAACATCGCAGTGACTATTG+TGG 0.611836 5_2:-14833882 Msa0759280:CDS
ATTATAAGGAGGCTCTAGAA+GGG 0.613050 5_2:-14834993 Msa0759280:CDS
GACACTGATAGTGATACAGA+TGG 0.614810 5_2:-14837016 Msa0759280:CDS
GCTACTTTGAGTTACTTCCA+AGG 0.617165 5_2:-14836260 Msa0759280:CDS
GTTGATAAACAAGTTGCTAG+TGG 0.617676 5_2:-14836434 Msa0759280:CDS
TGATAGTGATACAGATGGGG+TGG 0.618742 5_2:-14837011 Msa0759280:CDS
CATGTCCATTTCCCTCATGA+CGG 0.619225 5_2:+14834941 None:intergenic
GATGATAGGGTAGTTGTCGG+AGG 0.619255 5_2:+14834100 None:intergenic
AAATGGTTGTTTCTCCATGG+TGG 0.622494 5_2:+14836048 None:intergenic
CATTGAACGAGATGAAGATG+AGG 0.623154 5_2:-14835358 Msa0759280:CDS
ACATAGTGATACAGATGAGG+TGG 0.623452 5_2:-14837062 Msa0759280:CDS
CCACTGATGGTGAATGTCAA+AGG 0.624002 5_2:-14834856 Msa0759280:CDS
TATGGTTCCAACTTGCACAA+TGG 0.627946 5_2:+14834200 None:intergenic
TTTCACGGATCATCAGGCAA+GGG 0.637022 5_2:-14836309 Msa0759280:CDS
AAAAGCGGTGATTACTGATG+GGG 0.637407 5_2:-14835937 Msa0759280:CDS
TTTAGTGGATATAACCACCA+TGG 0.638528 5_2:-14836062 Msa0759280:CDS
AGAACGACATCAATCTGTGA+AGG 0.639278 5_2:-14835627 Msa0759280:CDS
CCTTTCTTACTTCAAACCCG+TGG 0.639832 5_2:+14836720 None:intergenic
CATTGCAAACACCATTACCA+TGG 0.642035 5_2:+14836867 None:intergenic
TATGGGGTGCCAAATAATGG+TGG 0.646881 5_2:-14834142 Msa0759280:CDS
AATGGTGTTTGCAATGAGCA+AGG 0.648235 5_2:-14836860 Msa0759280:CDS
GCTAGTGGTCTACATTCGCA+AGG 0.649023 5_2:-14836419 Msa0759280:CDS
AACAATAGAGACATACAAGT+GGG 0.649289 5_2:-14836000 Msa0759280:CDS
ATCAAATGGACAAAACGCAA+AGG 0.651947 5_2:+14836482 None:intergenic
AAAATGACCATGTTTCGTCG+AGG 0.652304 5_2:-14835072 Msa0759280:CDS
TGACCTATCAAACAACAGGT+GGG 0.653955 5_2:-14835721 Msa0759280:CDS
ATGGTGTTTGCAATGAGCAA+GGG 0.657603 5_2:-14836859 Msa0759280:CDS
TCTGTAGCAGTGTCAAATGG+TGG 0.657609 5_2:-14834529 Msa0759280:CDS
TGATGGAGATGCAGACGGTG+TGG 0.660310 5_2:-14836897 Msa0759280:CDS
ACACTGATAGTGATACAGAT+GGG 0.663559 5_2:-14837015 Msa0759280:CDS
TGTCGTGTCTGAAGATCAAG+GGG 0.668190 5_2:-14834731 Msa0759280:CDS
AAACAATAGAGACATACAAG+TGG 0.671084 5_2:-14836001 Msa0759280:CDS
TGCCATGAAGTATGACAACA+TGG 0.671427 5_2:-14835190 Msa0759280:CDS
GTATGACAAACATCCAAAAG+CGG 0.671446 5_2:-14835952 Msa0759280:CDS
TGAATGTCAAAGGTGCCCGT+AGG 0.674674 5_2:-14834846 Msa0759280:CDS
CAATTAGTATGCAATAAGGA+GGG 0.675920 5_2:-14836656 Msa0759280:CDS
TGTAGACGCTTTGAACGTAG+AGG 0.677604 5_2:-14835237 Msa0759280:CDS
CAATGTTCCGAGTTATACAC+CGG 0.682160 5_2:-14834026 Msa0759280:intron
CATTTGACACTGCTACAGAG+TGG 0.682675 5_2:+14834533 None:intergenic
GAGCGCAAGTGATTATAAGG+AGG 0.683366 5_2:-14835004 Msa0759280:CDS
TGTTGCGAATGATCCACTGA+TGG 0.687875 5_2:-14834869 Msa0759280:CDS
GACGGTGTGGAATTTAACCA+TGG 0.688761 5_2:-14836884 Msa0759280:CDS
TGAGGGAAATGGACATGACG+AGG 0.691164 5_2:-14834935 Msa0759280:CDS
CGTACTGATGGAGATGCAGA+CGG 0.711480 5_2:-14836902 Msa0759280:CDS
ACATAGACTATTGAACGCAG+CGG 0.718507 5_2:+14835041 None:intergenic
CACTGATAGTGATACAGATG+GGG 0.724418 5_2:-14837014 Msa0759280:CDS
TGTACATAGTGATACAGATG+AGG 0.739686 5_2:-14837065 Msa0759280:CDS
AATTATGCAAGAAATCCACA+AGG 0.743577 5_2:+14835562 None:intergenic
GGATATGGAATTCAACACAG+AGG 0.763384 5_2:-14836780 Msa0759280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAATTGTATATGATATTT+TGG - chr5_2:14836365-14836384 Msa0759280:CDS 15.0%
!!! AATTATTTTAATATGCACTA+TGG - chr5_2:14836809-14836828 Msa0759280:CDS 15.0%
!!! ATTATTTTAATATGCACTAT+GGG - chr5_2:14836810-14836829 Msa0759280:CDS 15.0%
!!! TCATAAACTGTTTTAAATAT+AGG + chr5_2:14837038-14837057 None:intergenic 15.0%
!! AAAGGACTTATATAATTTCA+CGG - chr5_2:14834645-14834664 Msa0759280:intron 20.0%
!! AAAGTTTAAAGATGTCAAAA+AGG - chr5_2:14835560-14835579 Msa0759280:CDS 20.0%
!!! ATTTTCAAGGTTAATGTATT+TGG - chr5_2:14835648-14835667 Msa0759280:CDS 20.0%
!!! TTATTTTAATATGCACTATG+GGG - chr5_2:14836811-14836830 Msa0759280:CDS 20.0%
! GATGGAAAAAATAAGAAGAA+TGG - chr5_2:14834343-14834362 Msa0759280:intron 25.0%
! TATCAACTTCACTCAATTTA+CGG + chr5_2:14834522-14834541 None:intergenic 25.0%
! ATATCCACTAAAAATGACAA+GGG + chr5_2:14834899-14834918 None:intergenic 25.0%
! TATATCCACTAAAAATGACA+AGG + chr5_2:14834900-14834919 None:intergenic 25.0%
! AATTAAAGCACAAGCAAAAA+TGG + chr5_2:14834941-14834960 None:intergenic 25.0%
!!! GGTTCTAAATTTGTTTTCAA+AGG - chr5_2:14834990-14835009 Msa0759280:CDS 25.0%
! GGTGGGTAAAAAAAATTTAT+CGG - chr5_2:14835265-14835284 Msa0759280:CDS 25.0%
!! GACATCTTTAAACTTTTTAC+CGG + chr5_2:14835556-14835575 None:intergenic 25.0%
!! CAAAAAAGATGTAGCTTTTT+CGG - chr5_2:14835671-14835690 Msa0759280:CDS 25.0%
!! TTTTTCATCATTACCTTCTT+GGG + chr5_2:14835880-14835899 None:intergenic 25.0%
!!! TTTTTTCATCATTACCTTCT+TGG + chr5_2:14835881-14835900 None:intergenic 25.0%
!! TCAATGTTGATTGTGATATT+TGG + chr5_2:14836602-14836621 None:intergenic 25.0%
! CAGCAAATATACTATAACAA+AGG + chr5_2:14836635-14836654 None:intergenic 25.0%
!!! TTGTTTTGATTGTTGTAGAA+TGG - chr5_2:14836698-14836717 Msa0759280:CDS 25.0%
! TAAACAATCAAAATACTCAC+CGG + chr5_2:14836965-14836984 None:intergenic 25.0%
GTATCACTATGTACATCAAA+AGG + chr5_2:14833899-14833918 None:intergenic 30.0%
ATGTGACAGATGATGATATA+AGG - chr5_2:14834167-14834186 Msa0759280:CDS 30.0%
TGTGACAGATGATGATATAA+GGG - chr5_2:14834168-14834187 Msa0759280:CDS 30.0%
AGATGATGATATAAGGGATA+TGG - chr5_2:14834174-14834193 Msa0759280:CDS 30.0%
GTATTGTTTGCAAAATACCA+CGG - chr5_2:14834232-14834251 Msa0759280:CDS 30.0%
TATTGTTTGCAAAATACCAC+GGG - chr5_2:14834233-14834252 Msa0759280:CDS 30.0%
TCAATTAGTATGCAATAAGG+AGG - chr5_2:14834312-14834331 Msa0759280:intron 30.0%
CAATTAGTATGCAATAAGGA+GGG - chr5_2:14834313-14834332 Msa0759280:intron 30.0%
TCACTCAATTTACGGTAATT+AGG + chr5_2:14834514-14834533 None:intergenic 30.0%
TGTAAGAACATGTCATATCT+TGG - chr5_2:14834573-14834592 Msa0759280:intron 30.0%
GTAAGAACATGTCATATCTT+GGG - chr5_2:14834574-14834593 Msa0759280:intron 30.0%
!!! TCATATCTTGGGTTTTTTGA+TGG - chr5_2:14834585-14834604 Msa0759280:intron 30.0%
!!! CATATCTTGGGTTTTTTGAT+GGG - chr5_2:14834586-14834605 Msa0759280:intron 30.0%
ATATAATTTCACGGATCATC+AGG - chr5_2:14834654-14834673 Msa0759280:intron 30.0%
CAAGGGTTAAAATACAAGAT+GGG - chr5_2:14834677-14834696 Msa0759280:intron 30.0%
AAGGGTTAAAATACAAGATG+GGG - chr5_2:14834678-14834697 Msa0759280:intron 30.0%
GAGTTTACATTTACAGATGA+TGG - chr5_2:14834763-14834782 Msa0759280:CDS 30.0%
!!! ACGCCTAGAAAACATTTTTT+GGG - chr5_2:14834786-14834805 Msa0759280:CDS 30.0%
ATACTTGTTCTTTCTATAGG+TGG + chr5_2:14834869-14834888 None:intergenic 30.0%
ACCTATAGAAAGAACAAGTA+TGG - chr5_2:14834868-14834887 Msa0759280:CDS 30.0%
TCCATACTTGTTCTTTCTAT+AGG + chr5_2:14834872-14834891 None:intergenic 30.0%
TATCCACTAAAAATGACAAG+GGG + chr5_2:14834898-14834917 None:intergenic 30.0%
AAACAATAGAGACATACAAG+TGG - chr5_2:14834968-14834987 Msa0759280:CDS 30.0%
AACAATAGAGACATACAAGT+GGG - chr5_2:14834969-14834988 Msa0759280:CDS 30.0%
! TTTTGGATGTTTGTCATACA+TGG + chr5_2:14835016-14835035 None:intergenic 30.0%
!! ATTTTGACCTATCAAACAAC+AGG - chr5_2:14835244-14835263 Msa0759280:CDS 30.0%
TTCTAACACCACACAAAAAA+TGG + chr5_2:14835326-14835345 None:intergenic 30.0%
AATTATGCAAGAAATCCACA+AGG + chr5_2:14835410-14835429 None:intergenic 30.0%
!!! TGGAAACATTGTCATTTTCA+AGG - chr5_2:14835635-14835654 Msa0759280:CDS 30.0%
!! TAAATTTGTTGCGAGTGATA+AGG - chr5_2:14836067-14836086 Msa0759280:CDS 30.0%
ATCATTCGCAACAAATTTGT+TGG + chr5_2:14836093-14836112 None:intergenic 30.0%
TGTTCTAATTGTAGAAATCC+TGG - chr5_2:14836176-14836195 Msa0759280:CDS 30.0%
GTTCTAATTGTAGAAATCCT+GGG - chr5_2:14836177-14836196 Msa0759280:CDS 30.0%
GATTCATTATCAGACTCAAT+AGG + chr5_2:14836275-14836294 None:intergenic 30.0%
AACAAATACAAGCACTAGAA+AGG - chr5_2:14836559-14836578 Msa0759280:CDS 30.0%
ACAAATACAAGCACTAGAAA+GGG - chr5_2:14836560-14836579 Msa0759280:CDS 30.0%
! ATTTTTCATGTCCAAAGTCA+CGG + chr5_2:14836730-14836749 None:intergenic 30.0%
AAAACTGATCCACCATTATT+TGG + chr5_2:14836839-14836858 None:intergenic 30.0%
AGTGTTAGAATGTGATGATA+GGG + chr5_2:14836885-14836904 None:intergenic 30.0%
TGTACATAGTGATACAGATG+AGG - chr5_2:14833904-14833923 Msa0759280:CDS 35.0%
! ACACTGATAGTGATACAGAT+GGG - chr5_2:14833954-14833973 Msa0759280:intron 35.0%
GTGGAATTTAACCATGGTAA+TGG - chr5_2:14834091-14834110 Msa0759280:CDS 35.0%
ATGTGAAGCGTGATTCTAAT+GGG - chr5_2:14834275-14834294 Msa0759280:intron 35.0%
GCGTCAATTAGTATGCAATA+AGG - chr5_2:14834309-14834328 Msa0759280:intron 35.0%
!! AATAAGGAGGGTGTTAATGA+TGG - chr5_2:14834325-14834344 Msa0759280:intron 35.0%
ATCAAATGGACAAAACGCAA+AGG + chr5_2:14834490-14834509 None:intergenic 35.0%
TACGGTAATTAGGCATCAAA+TGG + chr5_2:14834504-14834523 None:intergenic 35.0%
! GTTGATAAACAAGTTGCTAG+TGG - chr5_2:14834535-14834554 Msa0759280:CDS 35.0%
!!! GGTTTTTTGATGGGTCAAAA+GGG - chr5_2:14834595-14834614 Msa0759280:intron 35.0%
AGGACTTGGGTTTATCAAAA+AGG - chr5_2:14834627-14834646 Msa0759280:intron 35.0%
GCAAGGGTTAAAATACAAGA+TGG - chr5_2:14834676-14834695 Msa0759280:intron 35.0%
! TTTGAGTTACTTCCAAGGTA+AGG - chr5_2:14834714-14834733 Msa0759280:CDS 35.0%
!!! GACGCCTAGAAAACATTTTT+TGG - chr5_2:14834785-14834804 Msa0759280:CDS 35.0%
! CTGCCCAAAAAATGTTTTCT+AGG + chr5_2:14834792-14834811 None:intergenic 35.0%
! AGGTTTGACTATGAGTGTTT+TGG - chr5_2:14834823-14834842 Msa0759280:CDS 35.0%
!!! AAACCCCTTGTCATTTTTAG+TGG - chr5_2:14834892-14834911 Msa0759280:CDS 35.0%
! TTTAGTGGATATAACCACCA+TGG - chr5_2:14834907-14834926 Msa0759280:CDS 35.0%
!! CAAAAATGGTTGTTTCTCCA+TGG + chr5_2:14834927-14834946 None:intergenic 35.0%
GTATGACAAACATCCAAAAG+CGG - chr5_2:14835017-14835036 Msa0759280:CDS 35.0%
GAGTTGTGTTTCCAAATACA+AGG - chr5_2:14835079-14835098 Msa0759280:CDS 35.0%
!!! TCAAACTTTTCAGGTGTACA+AGG + chr5_2:14835198-14835217 None:intergenic 35.0%
! CACTCAGATTCAAACTTTTC+AGG + chr5_2:14835207-14835226 None:intergenic 35.0%
CTGAAAAGTTTGAATCTGAG+TGG - chr5_2:14835205-14835224 Msa0759280:CDS 35.0%
! TTTATCGGTTGAAGAGAATG+TGG - chr5_2:14835280-14835299 Msa0759280:CDS 35.0%
! TTATCGGTTGAAGAGAATGT+GGG - chr5_2:14835281-14835300 Msa0759280:CDS 35.0%
GAAGCTTCAAAGATATTCAC+CGG - chr5_2:14835534-14835553 Msa0759280:CDS 35.0%
ATTTAGTCTGCAACTTGTCA+AGG + chr5_2:14835701-14835720 None:intergenic 35.0%
CTTCATGGCACAAATAATGT+GGG + chr5_2:14835769-14835788 None:intergenic 35.0%
ACTTCATGGCACAAATAATG+TGG + chr5_2:14835770-14835789 None:intergenic 35.0%
ATCCATGTTGTCATACTTCA+TGG + chr5_2:14835784-14835803 None:intergenic 35.0%
! AAAGTCTGATTTGCAAGAGA+TGG - chr5_2:14835814-14835833 Msa0759280:CDS 35.0%
! TAAGAGCGCAAGTGATTATA+AGG - chr5_2:14835962-14835981 Msa0759280:CDS 35.0%
!! ATTATAAGGAGGCTCTAGAA+GGG - chr5_2:14835976-14835995 Msa0759280:CDS 35.0%
TAAGATAAATAGCCGTCATG+AGG - chr5_2:14836016-14836035 Msa0759280:CDS 35.0%
AAGATAAATAGCCGTCATGA+GGG - chr5_2:14836017-14836036 Msa0759280:CDS 35.0%
ATCACTCGCAACAAATTTAC+TGG + chr5_2:14836066-14836085 None:intergenic 35.0%
!!! TGTTGACTTTTTACTCCTAC+GGG + chr5_2:14836141-14836160 None:intergenic 35.0%
!!! GTGTTGACTTTTTACTCCTA+CGG + chr5_2:14836142-14836161 None:intergenic 35.0%
TTAGAACAACATCTTGTCCT+TGG + chr5_2:14836164-14836183 None:intergenic 35.0%
TATCATTACTCATAGACAGC+TGG + chr5_2:14836403-14836422 None:intergenic 35.0%
!! TGCTGAAAATTGTGTTGCTT+TGG - chr5_2:14836650-14836669 Msa0759280:CDS 35.0%
! TTTTTCATGTCCAAAGTCAC+GGG + chr5_2:14836729-14836748 None:intergenic 35.0%
! CAAGTTGGAACCATAATGTT+GGG - chr5_2:14836777-14836796 Msa0759280:CDS 35.0%
TAACACCATACCCAACATTA+TGG + chr5_2:14836790-14836809 None:intergenic 35.0%
CAGTGTTAGAATGTGATGAT+AGG + chr5_2:14836886-14836905 None:intergenic 35.0%
ACATAGTGATACAGATGAGG+TGG - chr5_2:14833907-14833926 Msa0759280:CDS 40.0%
! GACACTGATAGTGATACAGA+TGG - chr5_2:14833953-14833972 Msa0759280:intron 40.0%
! CACTGATAGTGATACAGATG+GGG - chr5_2:14833955-14833974 Msa0759280:intron 40.0%
AACAACGATGTTCGTACTGA+TGG - chr5_2:14834055-14834074 Msa0759280:CDS 40.0%
CATTGCAAACACCATTACCA+TGG + chr5_2:14834105-14834124 None:intergenic 40.0%
AATGGTGTTTGCAATGAGCA+AGG - chr5_2:14834109-14834128 Msa0759280:CDS 40.0%
ATGGTGTTTGCAATGAGCAA+GGG - chr5_2:14834110-14834129 Msa0759280:CDS 40.0%
CAAGGGAAAGAGAACTTTGA+TGG - chr5_2:14834127-14834146 Msa0759280:CDS 40.0%
GGATATGGAATTCAACACAG+AGG - chr5_2:14834189-14834208 Msa0759280:CDS 40.0%
GATGTGAAGCGTGATTCTAA+TGG - chr5_2:14834274-14834293 Msa0759280:intron 40.0%
TTCGAATGGTTAGCGTAGAT+CGG + chr5_2:14834372-14834391 None:intergenic 40.0%
CGCAGAACAATTAGTTCGAA+TGG + chr5_2:14834386-14834405 None:intergenic 40.0%
ACGACTTTGTTCGTACGATT+TGG - chr5_2:14834422-14834441 Msa0759280:CDS 40.0%
GCTAATTCATGGTTGTGAGA+GGG + chr5_2:14834466-14834485 None:intergenic 40.0%
AGCTAATTCATGGTTGTGAG+AGG + chr5_2:14834467-14834486 None:intergenic 40.0%
AACGCAAAGGAGCTAATTCA+TGG + chr5_2:14834477-14834496 None:intergenic 40.0%
!!! GGGTTTTTTGATGGGTCAAA+AGG - chr5_2:14834594-14834613 Msa0759280:intron 40.0%
!! TTTTTGATGGGTCAAAAGGG+TGG - chr5_2:14834598-14834617 Msa0759280:intron 40.0%
TGATAAACCCAAGTCCTTCA+TGG + chr5_2:14834624-14834643 None:intergenic 40.0%
! GCTACTTTGAGTTACTTCCA+AGG - chr5_2:14834709-14834728 Msa0759280:CDS 40.0%
TATCAGTATCAGCCTTACCT+TGG + chr5_2:14834729-14834748 None:intergenic 40.0%
TTTGGGCAGATTCTACTAGT+AGG - chr5_2:14834803-14834822 Msa0759280:CDS 40.0%
!! AAATGGTTGTTTCTCCATGG+TGG + chr5_2:14834924-14834943 None:intergenic 40.0%
CAAAAGCGGTGATTACTGAT+GGG - chr5_2:14835031-14835050 Msa0759280:CDS 40.0%
AAAAGCGGTGATTACTGATG+GGG - chr5_2:14835032-14835051 Msa0759280:CDS 40.0%
AGTTTGAATCTGAGTGGAAG+AGG - chr5_2:14835211-14835230 Msa0759280:CDS 40.0%
GTTTGAATCTGAGTGGAAGA+GGG - chr5_2:14835212-14835231 Msa0759280:CDS 40.0%
TTGACCTATCAAACAACAGG+TGG - chr5_2:14835247-14835266 Msa0759280:CDS 40.0%
TGACCTATCAAACAACAGGT+GGG - chr5_2:14835248-14835267 Msa0759280:CDS 40.0%
! TTACCCACCTGTTGTTTGAT+AGG + chr5_2:14835254-14835273 None:intergenic 40.0%
!!! ATGCGAGACCATTTTTTGTG+TGG - chr5_2:14835315-14835334 Msa0759280:CDS 40.0%
AGAACGACATCAATCTGTGA+AGG - chr5_2:14835342-14835361 Msa0759280:CDS 40.0%
AGATGTCAAAAAGGCGATAG+AGG - chr5_2:14835569-14835588 Msa0759280:CDS 40.0%
CATTGAACGAGATGAAGATG+AGG - chr5_2:14835611-14835630 Msa0759280:CDS 40.0%
GCACAAATAATGTGGGAACA+TGG + chr5_2:14835762-14835781 None:intergenic 40.0%
TGCCATGAAGTATGACAACA+TGG - chr5_2:14835779-14835798 Msa0759280:CDS 40.0%
CTCTTGCAAATCAGACTTTC+AGG + chr5_2:14835813-14835832 None:intergenic 40.0%
TTCATCATTACCTTCTTGGG+AGG + chr5_2:14835877-14835896 None:intergenic 40.0%
AAAATGACCATGTTTCGTCG+AGG - chr5_2:14835897-14835916 Msa0759280:CDS 40.0%
AAATGACCATGTTTCGTCGA+GGG - chr5_2:14835898-14835917 Msa0759280:CDS 40.0%
ACATAGACTATTGAACGCAG+CGG + chr5_2:14835931-14835950 None:intergenic 40.0%
!! GATTATAAGGAGGCTCTAGA+AGG - chr5_2:14835975-14835994 Msa0759280:CDS 40.0%
AAATGGACATGACGAGGATA+AGG - chr5_2:14836040-14836059 Msa0759280:CDS 40.0%
GGAGTAAAAAGTCAACACCA+AGG - chr5_2:14836144-14836163 Msa0759280:CDS 40.0%
CACTGGTATTACACGTAAGA+GGG - chr5_2:14836481-14836500 Msa0759280:CDS 40.0%
TATGGTTCCAACTTGCACAA+TGG + chr5_2:14836772-14836791 None:intergenic 40.0%
! GCAAGTTGGAACCATAATGT+TGG - chr5_2:14836776-14836795 Msa0759280:CDS 40.0%
TGGAACCATAATGTTGGGTA+TGG - chr5_2:14836782-14836801 Msa0759280:CDS 40.0%
TGTGATGATAGGGTAGTTGT+CGG + chr5_2:14836875-14836894 None:intergenic 40.0%
CAATGTTCCGAGTTATACAC+CGG - chr5_2:14836943-14836962 Msa0759280:CDS 40.0%
ATACTCACCGGTGTATAACT+CGG + chr5_2:14836953-14836972 None:intergenic 40.0%
TATGAGTGCGTTGAAGAATG+TGG - chr5_2:14837053-14837072 Msa0759280:CDS 40.0%
AAACATCGCAGTGACTATTG+TGG - chr5_2:14837087-14837106 Msa0759280:CDS 40.0%
! TGATAGTGATACAGATGGGG+TGG - chr5_2:14833958-14833977 Msa0759280:intron 45.0%
GACGGTGTGGAATTTAACCA+TGG - chr5_2:14834085-14834104 Msa0759280:CDS 45.0%
CCTTTCTTACTTCAAACCCG+TGG + chr5_2:14834252-14834271 None:intergenic 45.0%
! CCACGGGTTTGAAGTAAGAA+AGG - chr5_2:14834249-14834268 Msa0759280:CDS 45.0%
ATTTCACGGATCATCAGGCA+AGG - chr5_2:14834659-14834678 Msa0759280:intron 45.0%
TTTCACGGATCATCAGGCAA+GGG - chr5_2:14834660-14834679 Msa0759280:intron 45.0%
! CCATCAGTAATCACCGCTTT+TGG + chr5_2:14835033-14835052 None:intergenic 45.0%
CCAAAAGCGGTGATTACTGA+TGG - chr5_2:14835030-14835049 Msa0759280:CDS 45.0%
CAAAGGCGATGCCTTGTATT+TGG + chr5_2:14835093-14835112 None:intergenic 45.0%
AGCATTTCGATGCAAATGCC+AGG + chr5_2:14835118-14835137 None:intergenic 45.0%
!!! CCACAAGGCTGTTTTTGTGT+CGG + chr5_2:14835395-14835414 None:intergenic 45.0%
GTCAATGCAGTTGTCAGCAT+AGG + chr5_2:14835501-14835520 None:intergenic 45.0%
GAACGAGATGAAGATGAGGA+TGG - chr5_2:14835615-14835634 Msa0759280:CDS 45.0%
! TGTAGACGCTTTGAACGTAG+AGG - chr5_2:14835732-14835751 Msa0759280:CDS 45.0%
AATGACCATGTTTCGTCGAG+GGG - chr5_2:14835899-14835918 Msa0759280:CDS 45.0%
GAGCGCAAGTGATTATAAGG+AGG - chr5_2:14835965-14835984 Msa0759280:CDS 45.0%
AATAGCCGTCATGAGGGAAA+TGG - chr5_2:14836023-14836042 Msa0759280:CDS 45.0%
CATGTCCATTTCCCTCATGA+CGG + chr5_2:14836031-14836050 None:intergenic 45.0%
TGTTGCGAATGATCCACTGA+TGG - chr5_2:14836100-14836119 Msa0759280:CDS 45.0%
CCTTTGACATTCACCATCAG+TGG + chr5_2:14836116-14836135 None:intergenic 45.0%
! CCACTGATGGTGAATGTCAA+AGG - chr5_2:14836113-14836132 Msa0759280:CDS 45.0%
GAAATCCTGGGCACACAATA+CGG - chr5_2:14836189-14836208 Msa0759280:CDS 45.0%
!! TGTCCAGAACTTGCTGGAAT+TGG - chr5_2:14836215-14836234 Msa0759280:CDS 45.0%
ACACCAATTCCAGCAAGTTC+TGG + chr5_2:14836221-14836240 None:intergenic 45.0%
GTGTCGTGTCTGAAGATCAA+GGG - chr5_2:14836237-14836256 Msa0759280:CDS 45.0%
TGTCGTGTCTGAAGATCAAG+GGG - chr5_2:14836238-14836257 Msa0759280:CDS 45.0%
CATTTGACACTGCTACAGAG+TGG + chr5_2:14836439-14836458 None:intergenic 45.0%
CACTCTGTAGCAGTGTCAAA+TGG - chr5_2:14836437-14836456 Msa0759280:CDS 45.0%
TCTGTAGCAGTGTCAAATGG+TGG - chr5_2:14836440-14836459 Msa0759280:CDS 45.0%
! CGAGATGTTGAGAAAAGCAC+TGG - chr5_2:14836464-14836483 Msa0759280:CDS 45.0%
! GCACTGGTATTACACGTAAG+AGG - chr5_2:14836480-14836499 Msa0759280:CDS 45.0%
AAGACAGCCATTGTGCAAGT+TGG - chr5_2:14836762-14836781 Msa0759280:CDS 45.0%
! CACTATGGGGTGCCAAATAA+TGG - chr5_2:14836824-14836843 Msa0759280:CDS 45.0%
! TATGGGGTGCCAAATAATGG+TGG - chr5_2:14836827-14836846 Msa0759280:CDS 45.0%
ATGATAGGGTAGTTGTCGGA+GGG + chr5_2:14836871-14836890 None:intergenic 45.0%
TCTAACACTGCTCTGCATAC+TGG - chr5_2:14836896-14836915 Msa0759280:CDS 45.0%
ACTGGAACACACAATGTTCC+TGG - chr5_2:14836914-14836933 Msa0759280:CDS 45.0%
! CGTACTGATGGAGATGCAGA+CGG - chr5_2:14834067-14834086 Msa0759280:CDS 50.0%
GCTAGTGGTCTACATTCGCA+AGG - chr5_2:14834550-14834569 Msa0759280:CDS 50.0%
CCGACACAAAAACAGCCTTG+TGG - chr5_2:14835392-14835411 Msa0759280:CDS 50.0%
TCGTCATTTGCCTCCCAAGA+AGG - chr5_2:14835864-14835883 Msa0759280:CDS 50.0%
TGAGGGAAATGGACATGACG+AGG - chr5_2:14836034-14836053 Msa0759280:CDS 50.0%
!! TGAATGTCAAAGGTGCCCGT+AGG - chr5_2:14836123-14836142 Msa0759280:CDS 50.0%
! GGTGTCGTGTCTGAAGATCA+AGG - chr5_2:14836236-14836255 Msa0759280:CDS 50.0%
GTCTGAAGATCAAGGGGAGA+AGG - chr5_2:14836244-14836263 Msa0759280:CDS 50.0%
AATGGTGTGACCCGTGACTT+TGG - chr5_2:14836716-14836735 Msa0759280:CDS 50.0%
GTAGTTGTCGGAGGGAATTG+TGG + chr5_2:14836863-14836882 None:intergenic 50.0%
GATGATAGGGTAGTTGTCGG+AGG + chr5_2:14836872-14836891 None:intergenic 50.0%
CTCGGAACATTGTGTGTTCC+AGG + chr5_2:14836935-14836954 None:intergenic 50.0%
TGATGGAGATGCAGACGGTG+TGG - chr5_2:14834072-14834091 Msa0759280:CDS 55.0%
GGTCAAAAGGGTGGCCATGA+AGG - chr5_2:14834607-14834626 Msa0759280:intron 55.0%
AAGGGTGGCCATGAAGGACT+TGG - chr5_2:14834613-14834632 Msa0759280:intron 55.0%
AGGGTGGCCATGAAGGACTT+GGG - chr5_2:14834614-14834633 Msa0759280:intron 55.0%
GATGCAAATGCCAGGCGCAA+AGG + chr5_2:14835110-14835129 None:intergenic 55.0%
AAGTGCCCCTCGACGAAACA+TGG + chr5_2:14835907-14835926 None:intergenic 55.0%
CACGTCCGTATTGTGTGCCC+AGG + chr5_2:14836197-14836216 None:intergenic 60.0%
CGGACGTGTCCAGAACTTGC+TGG - chr5_2:14836209-14836228 Msa0759280:CDS 60.0%
!! CAAGGCATCGCCTTTGCGCC+TGG - chr5_2:14835097-14835116 Msa0759280:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 14833869 14837122 14833869 ID=Msa0759280;Name=Msa0759280
chr5_2 mRNA 14833869 14837122 14833869 ID=Msa0759280-mRNA-1;Parent=Msa0759280;Name=Msa0759280-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|0|0|0.25|1|1|4|0|993
chr5_2 exon 14834684 14837122 14834684 ID=Msa0759280-mRNA-1:exon:6763;Parent=Msa0759280-mRNA-1
chr5_2 exon 14834366 14834587 14834366 ID=Msa0759280-mRNA-1:exon:6762;Parent=Msa0759280-mRNA-1
chr5_2 exon 14834027 14834275 14834027 ID=Msa0759280-mRNA-1:exon:6761;Parent=Msa0759280-mRNA-1
chr5_2 exon 14833869 14833940 14833869 ID=Msa0759280-mRNA-1:exon:6760;Parent=Msa0759280-mRNA-1
chr5_2 CDS 14834684 14837122 14834684 ID=Msa0759280-mRNA-1:cds;Parent=Msa0759280-mRNA-1
chr5_2 CDS 14834366 14834587 14834366 ID=Msa0759280-mRNA-1:cds;Parent=Msa0759280-mRNA-1
chr5_2 CDS 14834027 14834275 14834027 ID=Msa0759280-mRNA-1:cds;Parent=Msa0759280-mRNA-1
chr5_2 CDS 14833869 14833940 14833869 ID=Msa0759280-mRNA-1:cds;Parent=Msa0759280-mRNA-1
Gene Sequence

>Msa0759280

ATGTCTACATATAGTGAAGCAACATTGCCTTTTGATGTACATAGTGATACAGATGAGGTGGAATTTAATAGTGTTAATGATATTGACACTGATAGTGATACAGATGGGGTGGAATTTAATAGTGTTAATGATGTTGACACTGATAGTGACACTGATAGTGATACTGAAGATATAAAAGTCAATAGCAACAACGATGTTCGTACTGATGGAGATGCAGACGGTGTGGAATTTAACCATGGTAATGGTGTTTGCAATGAGCAAGGGAAAGAGAACTTTGATGGCTACAAATGCATCAGTGATGTGACAGATGATGATATAAGGGATATGGAATTCAACACAGAGGATGAAGCTATAAAGTTTTATGTATTGTTTGCAAAATACCACGGGTTTGAAGTAAGAAAGGATGATGTGAAGCGTGATTCTAATGGGAACATTATTATGCGTCAATTAGTATGCAATAAGGAGGGTGTTAATGATGGAAAAAATAAGAAGAATGGCAGCCGATCTACGCTAACCATTCGAACTAATTGTTCTGCGAAACTTCGAGTAGTCTACGACTTTGTTCGTACGATTTGGAGAGTTTCTATTTTCAAACCCTCTCACAACCATGAATTAGCTCCTTTGCGTTTTGTCCATTTGATGCCTAATTACCGTAAATTGAGTGAAGTTGATAAACAAGTTGCTAGTGGTCTACATTCGCAAGGTGTAAGAACATGTCATATCTTGGGTTTTTTGATGGGTCAAAAGGGTGGCCATGAAGGACTTGGGTTTATCAAAAAGGACTTATATAATTTCACGGATCATCAGGCAAGGGTTAAAATACAAGATGGGGATACTTTTGCTACTTTGAGTTACTTCCAAGGTAAGGCTGATACTGATACATCATTCTTTTCAGAGTTTACATTTACAGATGATGGACGCCTAGAAAACATTTTTTGGGCAGATTCTACTAGTAGGTTTGACTATGAGTGTTTTGGCGATGTTGTTGCATTTGACACCACCTATAGAAAGAACAAGTATGGAAAACCCCTTGTCATTTTTAGTGGATATAACCACCATGGAGAAACAACCATTTTTGCTTGTGCTTTAATTTGTGATGAAACAATAGAGACATACAAGTGGGTTCTAAATTTGTTTTCAAAGGCCATGTATGACAAACATCCAAAAGCGGTGATTACTGATGGGGATAAATCAATGAGAAAAGCTATCAGAGTTGTGTTTCCAAATACAAGGCATCGCCTTTGCGCCTGGCATTTGCATCGAAATGCTGTTCAACATGTGAAGAATTCAAAATTTTTAGAAGAGTTCAAGTCACTGATGTATTCTCCTTGTACACCTGAAAAGTTTGAATCTGAGTGGAAGAGGGTTGTTGAAAATTTTGACCTATCAAACAACAGGTGGGTAAAAAAAATTTATCGGTTGAAGAGAATGTGGGCAAGTGCATACATGCGAGACCATTTTTTGTGTGGTGTTAGAACGACATCAATCTGTGAAGGAGTGAATTCATTTATCAAGAAGTATGTCCGACACAAAAACAGCCTTGTGGATTTCTTGCATAATTTTGAAAGAGCTCTCAAAGAATACAGACATAATGAATTATTGTCTGATTTCAAGTCGTTCTATTATCATCCTATGCTGACAACTGCATTGACTACTTTTGAGTCTGAAGCTTCAAAGATATTCACCGGTAAAAAGTTTAAAGATGTCAAAAAGGCGATAGAGGATGTTGTTGCATTATCAGTCATTGAACGAGATGAAGATGAGGATGGAAACATTGTCATTTTCAAGGTTAATGTATTTGGCAAAAAAGATGTAGCTTTTTCGGTATACCTTGACAAGTTGCAGACTAAATTTTTATGTGATTGTAGACGCTTTGAACGTAGAGGACTGCCATGTTCCCACATTATTTGTGCCATGAAGTATGACAACATGGATGTGTTTCCTGAAAGTCTGATTTGCAAGAGATGGACAAAGACTGCAAAGAGTGATCACATCTCGTCATTTGCCTCCCAAGAAGGTAATGATGAAAAAATGACCATGTTTCGTCGAGGGGCACTTGCCGCTGCGTTCAATAGTCTATGTGATGTTTCGTGTAAGAGCGCAAGTGATTATAAGGAGGCTCTAGAAGGGATTTATAGTCTGTGTAATAAGATAAATAGCCGTCATGAGGGAAATGGACATGACGAGGATAAGGCCAGTAAATTTGTTGCGAGTGATAAGGCCAACAAATTTGTTGCGAATGATCCACTGATGGTGAATGTCAAAGGTGCCCGTAGGAGTAAAAAGTCAACACCAAGGACAAGATGTTGTTCTAATTGTAGAAATCCTGGGCACACAATACGGACGTGTCCAGAACTTGCTGGAATTGGTGTCGTGTCTGAAGATCAAGGGGAGAAGGCTTCACCTATTGAGTCTGATAATGAATCAAAAAGTTTAAGTCTGTCTATGAGTAATGATATGAATGATGACATCCACTCTGTAGCAGTGTCAAATGGTGGACGAGATGTTGAGAAAAGCACTGGTATTACACGTAAGAGGGAATGTGACAATGTTGCATTTTCAACACAAGACTCTGTCAACAAAAGAAAAACAAAAACAAATACAAGCACTAGAAAGGGTGATGCTTGTGTGACTCCAAATATCACAATCAACATTGATAATAATGGTGTGACCCGTGACTTTGGACATGAAAAATCTACTCTCAAACAAGACAGCCATTGTGCAAGTTGGAACCATAATGTTGGGTATGGTGTTAATTATTTTAATATGCACTATGGGGTGCCAAATAATGGTGGATCAGTTTTTCCACAATTCCCTCCGACAACTACCCTATCATCACATTCTAACACTGCTCTGCATACTGGAACACACAATGTTCCTGGAACACACAATGTTCCGAGTTATACACCGTTTATGAGTGCGTTGAAGAATGTGGAGCTGTTAGCAAAACATCGCAGTGACTATTGTGGACCAAAACATTGA
Protein sequence

>Msa0759280

MSTYSEATLPFDVHSDTDEVEFNSVNDIDTDSDTDGVEFNSVNDVDTDSDTDSDTEDIKVNSNNDVRTDGDADGVEFNHGNGVCNEQGKENFDGYKCISDVTDDDIRDMEFNTEDEAIKFYVLFAKYHGFEVRKDDVKRDSNGNIIMRQLVCNKEGVNDGKNKKNGSRSTLTIRTNCSAKLRVVYDFVRTIWRVSIFKPSHNHELAPLRFVHLMPNYRKLSEVDKQVASGLHSQGVRTCHILGFLMGQKGGHEGLGFIKKDLYNFTDHQARVKIQDGDTFATLSYFQGKADTDTSFFSEFTFTDDGRLENIFWADSTSRFDYECFGDVVAFDTTYRKNKYGKPLVIFSGYNHHGETTIFACALICDETIETYKWVLNLFSKAMYDKHPKAVITDGDKSMRKAIRVVFPNTRHRLCAWHLHRNAVQHVKNSKFLEEFKSLMYSPCTPEKFESEWKRVVENFDLSNNRWVKKIYRLKRMWASAYMRDHFLCGVRTTSICEGVNSFIKKYVRHKNSLVDFLHNFERALKEYRHNELLSDFKSFYYHPMLTTALTTFESEASKIFTGKKFKDVKKAIEDVVALSVIERDEDEDGNIVIFKVNVFGKKDVAFSVYLDKLQTKFLCDCRRFERRGLPCSHIICAMKYDNMDVFPESLICKRWTKTAKSDHISSFASQEGNDEKMTMFRRGALAAAFNSLCDVSCKSASDYKEALEGIYSLCNKINSRHEGNGHDEDKASKFVASDKANKFVANDPLMVNVKGARRSKKSTPRTRCCSNCRNPGHTIRTCPELAGIGVVSEDQGEKASPIESDNESKSLSLSMSNDMNDDIHSVAVSNGGRDVEKSTGITRKRECDNVAFSTQDSVNKRKTKTNTSTRKGDACVTPNITINIDNNGVTRDFGHEKSTLKQDSHCASWNHNVGYGVNYFNMHYGVPNNGGSVFPQFPPTTTLSSHSNTALHTGTHNVPGTHNVPSYTPFMSALKNVELLAKHRSDYCGPKH*