Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458300 | AES87914.2 | 84.173 | 139 | 9 | 2 | 1 | 138 | 1 | 127 | 4.58e-64 | 201 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458300 | sp|P47925|CWPX_ARATH | 38.739 | 111 | 61 | 4 | 23 | 130 | 23 | 129 | 5.46e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458300 | G7JSH2 | 84.173 | 139 | 9 | 2 | 1 | 138 | 1 | 127 | 2.19e-64 | 201 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1420060 | Msa1458300 | 0.811354 | 7.301562e-51 | -8.615850e-47 |
| Msa1421550 | Msa1458300 | 0.831317 | 1.787778e-55 | -8.615850e-47 |
| Msa0669840 | Msa1458300 | 0.809898 | 1.506888e-50 | -8.615850e-47 |
| Msa0424330 | Msa1458300 | 0.804239 | 2.376992e-49 | -8.615850e-47 |
| Msa0451400 | Msa1458300 | 0.814420 | 1.554519e-51 | -8.615850e-47 |
| Msa0477460 | Msa1458300 | 0.814291 | 1.660320e-51 | -8.615850e-47 |
| Msa1037900 | Msa1458300 | 0.807015 | 6.211714e-50 | -8.615850e-47 |
| Msa1037910 | Msa1458300 | 0.813343 | 2.685545e-51 | -8.615850e-47 |
| Msa0359250 | Msa1458300 | 0.818704 | 1.706196e-52 | -8.615850e-47 |
| Msa0898730 | Msa1458300 | 0.805734 | 1.156931e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458300 | MtrunA17_Chr4g0022211 | 89.130 | 138 | 15 | 0 | 1 | 138 | 1 | 138 | 8.18e-66 | 196 |
| Msa1458300 | MtrunA17_Chr4g0022191 | 96.104 | 77 | 3 | 0 | 1 | 77 | 1 | 77 | 6.17e-51 | 157 |
| Msa1458300 | MtrunA17_Chr2g0324051 | 53.237 | 139 | 51 | 5 | 1 | 138 | 1 | 126 | 2.90e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458300 | AT2G20870.1 | 38.739 | 111 | 61 | 4 | 23 | 130 | 23 | 129 | 5.55e-13 | 62.4 |
Find 51 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCTAATAATCCATTTAT+TGG | 0.205431 | tig0039517:+14990 | Msa1458300:CDS |
| TACCCTGGCATTGGACATTT+AGG | 0.235381 | tig0039517:+14945 | Msa1458300:CDS |
| GTCGTCATTGTCGGAGTTCT+TGG | 0.244678 | tig0039517:-14874 | Msa1458300:intergenic |
| CCTCCAGTGCCACCAATAAA+TGG | 0.254443 | tig0039517:-15002 | Msa1458300:intergenic |
| CCACCAATAAATGGATTATT+AGG | 0.286771 | tig0039517:-14993 | Msa1458300:intergenic |
| CTCTTTCTTGTCGTCATTGT+CGG | 0.295441 | tig0039517:-14883 | Msa1458300:intergenic |
| GGAGGATCAGGTTCAGGTTT+AGG | 0.307374 | tig0039517:+15020 | Msa1458300:CDS |
| CCGCCACCACTGCCAGGATT+TGG | 0.317121 | tig0039517:-15140 | Msa1458300:intergenic |
| GGCACTGGAGGATCAGGTTC+AGG | 0.329383 | tig0039517:+15014 | Msa1458300:CDS |
| TTAGGATTTCCACCGAAATT+CGG | 0.332231 | tig0039517:+14963 | Msa1458300:CDS |
| TAGGATTTCCACCGAAATTC+GGG | 0.357846 | tig0039517:+14964 | Msa1458300:CDS |
| GGAACCTCAAAGCCGGGGTT+TGG | 0.358168 | tig0039517:-15119 | Msa1458300:intergenic |
| CCTGGCAGTGGTGGCGGCGG+TGG | 0.368789 | tig0039517:+15146 | Msa1458300:CDS |
| GGCTTTGAGGTTCCAAATCC+TGG | 0.373171 | tig0039517:+15128 | Msa1458300:CDS |
| ATTGGTGGCACTGGAGGATC+AGG | 0.397280 | tig0039517:+15008 | Msa1458300:CDS |
| TCAGGTCACAGTTACGTTCC+CGG | 0.409019 | tig0039517:+15077 | Msa1458300:CDS |
| TCAGGTTTAGGATCAGGATC+AGG | 0.415541 | tig0039517:+15032 | Msa1458300:CDS |
| TGTTCCAAACCCCGGCTTTG+AGG | 0.417503 | tig0039517:+15115 | Msa1458300:CDS |
| AATCCATTTATTGGTGGCAC+TGG | 0.434316 | tig0039517:+14999 | Msa1458300:CDS |
| TCAGGTTCAGGTTTAGGATC+AGG | 0.442027 | tig0039517:+15026 | Msa1458300:CDS |
| TTAGGAGTAAGCCCGAATTT+CGG | 0.445449 | tig0039517:-14975 | Msa1458300:intergenic |
| CCAAATCCTGGCAGTGGTGG+CGG | 0.451387 | tig0039517:+15140 | Msa1458300:CDS |
| ACAACACGGCAAGCAGTTGC+TGG | 0.458864 | tig0039517:+14840 | Msa1458300:CDS |
| GGAGCAGGATCAGGATCATC+AGG | 0.476965 | tig0039517:+15059 | Msa1458300:CDS |
| CCACCGCCGCCACCACTGCC+AGG | 0.483162 | tig0039517:-15146 | Msa1458300:intergenic |
| GGATTTGGAACCTCAAAGCC+GGG | 0.491389 | tig0039517:-15125 | Msa1458300:intergenic |
| CCTAATAATCCATTTATTGG+TGG | 0.494125 | tig0039517:+14993 | Msa1458300:CDS |
| AACAAATGTGTCATCGCCAC+CGG | 0.495697 | tig0039517:-15096 | Msa1458300:intergenic |
| CCATTTATTGGTGGCACTGG+AGG | 0.501560 | tig0039517:+15002 | Msa1458300:CDS |
| AATCCTAAATGTCCAATGCC+AGG | 0.501871 | tig0039517:-14948 | Msa1458300:intergenic |
| CGGAGTTCTTGGCAATGTTG+CGG | 0.505040 | tig0039517:-14863 | Msa1458300:intergenic |
| GAGGTTCCAAATCCTGGCAG+TGG | 0.507650 | tig0039517:+15134 | Msa1458300:CDS |
| AAACTACATTACCCTGGCAT+TGG | 0.520118 | tig0039517:+14936 | Msa1458300:CDS |
| TCAGGGATAGGAGCAGGATC+AGG | 0.520901 | tig0039517:+15050 | Msa1458300:CDS |
| AATCCTGGCAGTGGTGGCGG+CGG | 0.526554 | tig0039517:+15143 | Msa1458300:CDS |
| CGGTGGTAGAGTTGCATCAA+CGG | 0.529347 | tig0039517:+15163 | Msa1458300:CDS |
| AGGATTTGGAACCTCAAAGC+CGG | 0.531760 | tig0039517:-15126 | Msa1458300:intergenic |
| TCAGGATCAGGGATAGGAGC+AGG | 0.535138 | tig0039517:+15044 | Msa1458300:CDS |
| GACACATTTGTTCCAAACCC+CGG | 0.566437 | tig0039517:+15107 | Msa1458300:CDS |
| TTAGGATCAGGATCAGGGAT+AGG | 0.568662 | tig0039517:+15038 | Msa1458300:CDS |
| CAGGTTTAGGATCAGGATCA+GGG | 0.573753 | tig0039517:+15033 | Msa1458300:CDS |
| GTTCCAAATCCTGGCAGTGG+TGG | 0.581950 | tig0039517:+15137 | Msa1458300:CDS |
| GGAGTAAGCCCGAATTTCGG+TGG | 0.586534 | tig0039517:-14972 | Msa1458300:intergenic |
| ACAAATGTGTCATCGCCACC+GGG | 0.594099 | tig0039517:-15095 | Msa1458300:intergenic |
| CTGCTTGCCGTGTTGTGACA+AGG | 0.611217 | tig0039517:-14833 | Msa1458300:intergenic |
| ATCCTAAATGTCCAATGCCA+GGG | 0.617878 | tig0039517:-14947 | Msa1458300:intergenic |
| ATATCCTCCTTGTCACAACA+CGG | 0.657664 | tig0039517:+14826 | Msa1458300:CDS |
| CATGGTAAACTACATTACCC+TGG | 0.662493 | tig0039517:+14930 | Msa1458300:CDS |
| GATTTGGAACCTCAAAGCCG+GGG | 0.694571 | tig0039517:-15124 | Msa1458300:intergenic |
| GGTCACAGTTACGTTCCCGG+TGG | 0.780851 | tig0039517:+15080 | Msa1458300:CDS |
| CTTGCCGTGTTGTGACAAGG+AGG | 0.782718 | tig0039517:-14830 | Msa1458290:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTTTATTCAAGCATGATCA+TGG | + | tig0039517:14912-14931 | Msa1458300:CDS | 25.0% |
| ! | ACTCCTAATAATCCATTTAT+TGG | + | tig0039517:14990-15009 | Msa1458300:CDS | 25.0% |
| TCATGCTTGAATAAAAACTG+CGG | - | tig0039517:14909-14928 | Msa1458300:intergenic | 30.0% | |
| !! | CCACCAATAAATGGATTATT+AGG | - | tig0039517:14996-15015 | Msa1458300:intergenic | 30.0% |
| CCTAATAATCCATTTATTGG+TGG | + | tig0039517:14993-15012 | Msa1458300:CDS | 30.0% | |
| TTAGGATTTCCACCGAAATT+CGG | + | tig0039517:14963-14982 | Msa1458300:CDS | 35.0% | |
| ATATCCTCCTTGTCACAACA+CGG | + | tig0039517:14826-14845 | Msa1458300:CDS | 40.0% | |
| CTCTTTCTTGTCGTCATTGT+CGG | - | tig0039517:14886-14905 | Msa1458300:intergenic | 40.0% | |
| CATGGTAAACTACATTACCC+TGG | + | tig0039517:14930-14949 | Msa1458300:CDS | 40.0% | |
| AAACTACATTACCCTGGCAT+TGG | + | tig0039517:14936-14955 | Msa1458300:CDS | 40.0% | |
| ATCCTAAATGTCCAATGCCA+GGG | - | tig0039517:14950-14969 | Msa1458300:intergenic | 40.0% | |
| AATCCTAAATGTCCAATGCC+AGG | - | tig0039517:14951-14970 | Msa1458300:intergenic | 40.0% | |
| TAGGATTTCCACCGAAATTC+GGG | + | tig0039517:14964-14983 | Msa1458300:CDS | 40.0% | |
| TTAGGAGTAAGCCCGAATTT+CGG | - | tig0039517:14978-14997 | Msa1458300:intergenic | 40.0% | |
| ! | AATCCATTTATTGGTGGCAC+TGG | + | tig0039517:14999-15018 | Msa1458300:CDS | 40.0% |
| ! | TACCCTGGCATTGGACATTT+AGG | + | tig0039517:14945-14964 | Msa1458300:CDS | 45.0% |
| ! | TCAGGTTCAGGTTTAGGATC+AGG | + | tig0039517:15026-15045 | Msa1458300:CDS | 45.0% |
| ! | TCAGGTTTAGGATCAGGATC+AGG | + | tig0039517:15032-15051 | Msa1458300:CDS | 45.0% |
| ! | CAGGTTTAGGATCAGGATCA+GGG | + | tig0039517:15033-15052 | Msa1458300:CDS | 45.0% |
| TTAGGATCAGGATCAGGGAT+AGG | + | tig0039517:15038-15057 | Msa1458300:CDS | 45.0% | |
| AACAAATGTGTCATCGCCAC+CGG | - | tig0039517:15099-15118 | Msa1458300:intergenic | 45.0% | |
| GACACATTTGTTCCAAACCC+CGG | + | tig0039517:15107-15126 | Msa1458300:CDS | 45.0% | |
| ! | AGGATTTGGAACCTCAAAGC+CGG | - | tig0039517:15129-15148 | Msa1458300:intergenic | 45.0% |
| CGGAGTTCTTGGCAATGTTG+CGG | - | tig0039517:14866-14885 | Msa1458300:intergenic | 50.0% | |
| GTCGTCATTGTCGGAGTTCT+TGG | - | tig0039517:14877-14896 | Msa1458300:intergenic | 50.0% | |
| CCTCCAGTGCCACCAATAAA+TGG | - | tig0039517:15005-15024 | Msa1458300:intergenic | 50.0% | |
| ! | CCATTTATTGGTGGCACTGG+AGG | + | tig0039517:15002-15021 | Msa1458300:CDS | 50.0% |
| ! | GGAGGATCAGGTTCAGGTTT+AGG | + | tig0039517:15020-15039 | Msa1458300:CDS | 50.0% |
| TCAGGTCACAGTTACGTTCC+CGG | + | tig0039517:15077-15096 | Msa1458300:CDS | 50.0% | |
| ACAAATGTGTCATCGCCACC+GGG | - | tig0039517:15098-15117 | Msa1458300:intergenic | 50.0% | |
| ! | GATTTGGAACCTCAAAGCCG+GGG | - | tig0039517:15127-15146 | Msa1458300:intergenic | 50.0% |
| ! | GGATTTGGAACCTCAAAGCC+GGG | - | tig0039517:15128-15147 | Msa1458300:intergenic | 50.0% |
| !! | GGCTTTGAGGTTCCAAATCC+TGG | + | tig0039517:15128-15147 | Msa1458300:CDS | 50.0% |
| CGGTGGTAGAGTTGCATCAA+CGG | + | tig0039517:15163-15182 | Msa1458300:CDS | 50.0% | |
| CTTGCCGTGTTGTGACAAGG+AGG | - | tig0039517:14833-14852 | Msa1458300:intergenic | 55.0% | |
| CTGCTTGCCGTGTTGTGACA+AGG | - | tig0039517:14836-14855 | Msa1458300:intergenic | 55.0% | |
| ACAACACGGCAAGCAGTTGC+TGG | + | tig0039517:14840-14859 | Msa1458300:CDS | 55.0% | |
| GGAGTAAGCCCGAATTTCGG+TGG | - | tig0039517:14975-14994 | Msa1458300:intergenic | 55.0% | |
| ! | ATTGGTGGCACTGGAGGATC+AGG | + | tig0039517:15008-15027 | Msa1458300:CDS | 55.0% |
| TCAGGATCAGGGATAGGAGC+AGG | + | tig0039517:15044-15063 | Msa1458300:CDS | 55.0% | |
| TCAGGGATAGGAGCAGGATC+AGG | + | tig0039517:15050-15069 | Msa1458300:CDS | 55.0% | |
| GGAGCAGGATCAGGATCATC+AGG | + | tig0039517:15059-15078 | Msa1458300:CDS | 55.0% | |
| TGTTCCAAACCCCGGCTTTG+AGG | + | tig0039517:15115-15134 | Msa1458300:CDS | 55.0% | |
| GAGGTTCCAAATCCTGGCAG+TGG | + | tig0039517:15134-15153 | Msa1458300:CDS | 55.0% | |
| GTTCCAAATCCTGGCAGTGG+TGG | + | tig0039517:15137-15156 | Msa1458300:CDS | 55.0% | |
| ! | GGCACTGGAGGATCAGGTTC+AGG | + | tig0039517:15014-15033 | Msa1458300:CDS | 60.0% |
| GGTCACAGTTACGTTCCCGG+TGG | + | tig0039517:15080-15099 | Msa1458300:CDS | 60.0% | |
| GGAACCTCAAAGCCGGGGTT+TGG | - | tig0039517:15122-15141 | Msa1458300:intergenic | 60.0% | |
| CCAAATCCTGGCAGTGGTGG+CGG | + | tig0039517:15140-15159 | Msa1458300:CDS | 60.0% | |
| CCGCCACCACTGCCAGGATT+TGG | - | tig0039517:15143-15162 | Msa1458300:intergenic | 65.0% | |
| !! | AATCCTGGCAGTGGTGGCGG+CGG | + | tig0039517:15143-15162 | Msa1458300:CDS | 65.0% |
| ! | CCACCGCCGCCACCACTGCC+AGG | - | tig0039517:15149-15168 | Msa1458300:intergenic | 80.0% |
| !!! | CCTGGCAGTGGTGGCGGCGG+TGG | + | tig0039517:15146-15165 | Msa1458300:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0039517 | gene | 14780 | 15196 | 14780 | ID=Msa1458300;Name=Msa1458300 |
| tig0039517 | mRNA | 14780 | 15196 | 14780 | ID=Msa1458300-mRNA-1;Parent=Msa1458300;Name=Msa1458300-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|138 |
| tig0039517 | exon | 14780 | 15196 | 14780 | ID=Msa1458300-mRNA-1:exon:21681;Parent=Msa1458300-mRNA-1 |
| tig0039517 | CDS | 14780 | 15196 | 14780 | ID=Msa1458300-mRNA-1:cds;Parent=Msa1458300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
