AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1461190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461190 MtrunA17_Chr1g0200151 95.238 252 11 1 1 251 1 252 2.51e-153 426
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 48 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTTCCGACACCGGAAAA+TGG 0.187842 tig0041342:-6006 Msa1461190:CDS
TGAGGTGAAATTGTGGAATT+TGG 0.256995 tig0041342:+6492 None:intergenic
TTCCACAATTTCACCTCAAC+GGG 0.312827 tig0041342:-6487 Msa1461190:CDS
AGAGGAATAGGAATAGTAAA+AGG 0.330599 tig0041342:-5963 Msa1461190:CDS
ATGGTGATCCTTGCTGTAAT+TGG 0.353696 tig0041342:+6455 None:intergenic
TTGTTAAGAGTAAGAGGAAT+AGG 0.359418 tig0041342:-5975 Msa1461190:CDS
GGAACCATTACTAGATTTCA+TGG 0.377121 tig0041342:+6269 None:intergenic
TGTTTCCGACACCGGAAAAT+GGG 0.386344 tig0041342:-6005 Msa1461190:CDS
AAGAGGTTGTTAAGAGTAAG+AGG 0.411804 tig0041342:-5981 Msa1461190:CDS
CATAGCTAGCTTGAAGAGTA+AGG 0.421195 tig0041342:-6247 Msa1461190:CDS
TTGAAGAGTAAGGGAAATGA+AGG 0.446303 tig0041342:-6237 Msa1461190:CDS
GGAAATGAAGGAGAAGTAAA+GGG 0.448337 tig0041342:-6225 Msa1461190:CDS
TTGGAATGTCGTCGAGTTCG+AGG 0.453818 tig0041342:-6101 Msa1461190:CDS
TTCTACTCAGATCAAGAAGC+AGG 0.454222 tig0041342:-6591 Msa1461190:CDS
GTCACTAAGTTGCATCTCCT+TGG 0.456940 tig0041342:+6071 None:intergenic
GGGAAATGAAGGAGAAGTAA+AGG 0.459006 tig0041342:-6226 Msa1461190:CDS
TGCTGGTGACTACACATCAA+TGG 0.471580 tig0041342:-6181 Msa1461190:CDS
GCTGGTGACTACACATCAAT+GGG 0.479840 tig0041342:-6180 Msa1461190:CDS
AACATGTTGATGATCTTTGA+AGG 0.488709 tig0041342:+6338 None:intergenic
CTTACTCTTCAAGCTAGCTA+TGG 0.509662 tig0041342:+6248 None:intergenic
ATAGGAATAGTAAAAGGATG+TGG 0.514498 tig0041342:-5957 Msa1461190:CDS
ACTTGCTAATGATGTAGTAA+AGG 0.515795 tig0041342:-5887 Msa1461190:CDS
GAAGTCCAACAAAATCTAGA+TGG 0.518576 tig0041342:-6137 Msa1461190:CDS
ATGGCCTTTGAATTTGAAGT+GGG 0.521547 tig0041342:+6288 None:intergenic
CATGGCCTTTGAATTTGAAG+TGG 0.528240 tig0041342:+6287 None:intergenic
GGAATTTGGAAGGTCACAAA+GGG 0.536647 tig0041342:+6506 None:intergenic
GAAATAGACAGAGCAGAAGC+CGG 0.555893 tig0041342:-6044 Msa1461190:CDS
TAAACCCACTTCAAATTCAA+AGG 0.577655 tig0041342:-6292 Msa1461190:CDS
ATTCCACAATTTCACCTCAA+CGG 0.579273 tig0041342:-6488 Msa1461190:CDS
ATAGCTAGCTTGAAGAGTAA+GGG 0.580739 tig0041342:-6246 Msa1461190:CDS
GACACCGGAAAATGGGAAAG+AGG 0.585817 tig0041342:-5998 Msa1461190:CDS
ACATCGTCATCGGCTCCCTC+CGG 0.586108 tig0041342:+6025 None:intergenic
CATGACAACATAATCTTCTG+TGG 0.586506 tig0041342:-6372 Msa1461190:CDS
ACATGTTGATGATCTTTGAA+GGG 0.588773 tig0041342:+6339 None:intergenic
TGGAATTTGGAAGGTCACAA+AGG 0.590193 tig0041342:+6505 None:intergenic
GAGTTCGAGGATGTCTTCCA+AGG 0.590470 tig0041342:-6088 Msa1461190:CDS
GTGAAATTGTGGAATTTGGA+AGG 0.591726 tig0041342:+6496 None:intergenic
ACCCCGTTGAGGTGAAATTG+TGG 0.594385 tig0041342:+6485 None:intergenic
ATAGACAGAGCAGAAGCCGG+AGG 0.610980 tig0041342:-6041 Msa1461190:CDS
CTACACATCAATGGGAGGGA+AGG 0.612457 tig0041342:-6172 Msa1461190:CDS
AAGGCCATGAAATCTAGTAA+TGG 0.615347 tig0041342:-6273 Msa1461190:CDS
GGTGTCGGAAACATCGTCAT+CGG 0.617855 tig0041342:+6015 None:intergenic
TAGACAGAGCAGAAGCCGGA+GGG 0.622934 tig0041342:-6040 Msa1461190:CDS
GATGACGATGTTTCCGACAC+CGG 0.635603 tig0041342:-6013 Msa1461190:CDS
TCTACTCAGATCAAGAAGCA+GGG 0.638478 tig0041342:-6590 Msa1461190:CDS
GTGACTACACATCAATGGGA+GGG 0.664793 tig0041342:-6176 Msa1461190:CDS
GGTGACTACACATCAATGGG+AGG 0.668735 tig0041342:-6177 Msa1461190:CDS
TCCACAATTTCACCTCAACG+GGG 0.743576 tig0041342:-6486 Msa1461190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACCATCTAGATTTTGT+TGG + tig0041342:6350-6369 None:intergenic 25.0%
!!! AGATGGTTTTTGTTTATGTT+TGG - tig0041342:6359-6378 Msa1461190:CDS 25.0%
!!! GATTTTGAAGTCAATTTCAT+TGG - tig0041342:6547-6566 Msa1461190:CDS 25.0%
!!! AAGTCAATTTCATTGGTTTT+AGG - tig0041342:6554-6573 Msa1461190:CDS 25.0%
!! AAAAACCATCTAGATTTTGT+TGG + tig0041342:6350-6369 None:intergenic 25.0%
!!! AGATGGTTTTTGTTTATGTT+TGG - tig0041342:6359-6378 Msa1461190:CDS 25.0%
!!! GATTTTGAAGTCAATTTCAT+TGG - tig0041342:6547-6566 Msa1461190:CDS 25.0%
!!! AAGTCAATTTCATTGGTTTT+AGG - tig0041342:6554-6573 Msa1461190:CDS 25.0%
! TGATTTTTCCAATTACAGCA+AGG - tig0041342:6016-6035 Msa1461190:CDS 30.0%
AGTTATCATCATCATCATCA+TGG + tig0041342:6046-6065 None:intergenic 30.0%
!! ACATGTTGATGATCTTTGAA+GGG + tig0041342:6143-6162 None:intergenic 30.0%
!! AACATGTTGATGATCTTTGA+AGG + tig0041342:6144-6163 None:intergenic 30.0%
!!! GCACAATTTTTTTGGTTATG+AGG + tig0041342:6167-6186 None:intergenic 30.0%
!!! TGGGTTTAGCACAATTTTTT+TGG + tig0041342:6175-6194 None:intergenic 30.0%
TAAACCCACTTCAAATTCAA+AGG - tig0041342:6187-6206 Msa1461190:CDS 30.0%
! TTGAATGTAAAGAGTTTTGC+TGG - tig0041342:6281-6300 Msa1461190:CDS 30.0%
TTGTTAAGAGTAAGAGGAAT+AGG - tig0041342:6504-6523 Msa1461190:CDS 30.0%
AGAGGAATAGGAATAGTAAA+AGG - tig0041342:6516-6535 Msa1461190:CDS 30.0%
ATAGGAATAGTAAAAGGATG+TGG - tig0041342:6522-6541 Msa1461190:CDS 30.0%
! ACTTGCTAATGATGTAGTAA+AGG - tig0041342:6592-6611 Msa1461190:CDS 30.0%
! TGATTTTTCCAATTACAGCA+AGG - tig0041342:6016-6035 Msa1461190:CDS 30.0%
AGTTATCATCATCATCATCA+TGG + tig0041342:6046-6065 None:intergenic 30.0%
!! ACATGTTGATGATCTTTGAA+GGG + tig0041342:6143-6162 None:intergenic 30.0%
!! AACATGTTGATGATCTTTGA+AGG + tig0041342:6144-6163 None:intergenic 30.0%
!!! GCACAATTTTTTTGGTTATG+AGG + tig0041342:6167-6186 None:intergenic 30.0%
!!! TGGGTTTAGCACAATTTTTT+TGG + tig0041342:6175-6194 None:intergenic 30.0%
TAAACCCACTTCAAATTCAA+AGG - tig0041342:6187-6206 Msa1461190:CDS 30.0%
! TTGAATGTAAAGAGTTTTGC+TGG - tig0041342:6281-6300 Msa1461190:CDS 30.0%
TTGTTAAGAGTAAGAGGAAT+AGG - tig0041342:6504-6523 Msa1461190:CDS 30.0%
AGAGGAATAGGAATAGTAAA+AGG - tig0041342:6516-6535 Msa1461190:CDS 30.0%
ATAGGAATAGTAAAAGGATG+TGG - tig0041342:6522-6541 Msa1461190:CDS 30.0%
! ACTTGCTAATGATGTAGTAA+AGG - tig0041342:6592-6611 Msa1461190:CDS 30.0%
GTGAAATTGTGGAATTTGGA+AGG + tig0041342:5986-6005 None:intergenic 35.0%
TGAGGTGAAATTGTGGAATT+TGG + tig0041342:5990-6009 None:intergenic 35.0%
ATTCCACAATTTCACCTCAA+CGG - tig0041342:5991-6010 Msa1461190:CDS 35.0%
CATGACAACATAATCTTCTG+TGG - tig0041342:6107-6126 Msa1461190:CDS 35.0%
! ATGGCCTTTGAATTTGAAGT+GGG + tig0041342:6194-6213 None:intergenic 35.0%
AAGGCCATGAAATCTAGTAA+TGG - tig0041342:6206-6225 Msa1461190:CDS 35.0%
GGAACCATTACTAGATTTCA+TGG + tig0041342:6213-6232 None:intergenic 35.0%
! ATAGCTAGCTTGAAGAGTAA+GGG - tig0041342:6233-6252 Msa1461190:CDS 35.0%
TTGAAGAGTAAGGGAAATGA+AGG - tig0041342:6242-6261 Msa1461190:CDS 35.0%
GGAAATGAAGGAGAAGTAAA+GGG - tig0041342:6254-6273 Msa1461190:CDS 35.0%
GAAGTCCAACAAAATCTAGA+TGG - tig0041342:6342-6361 Msa1461190:CDS 35.0%
AAGAGGTTGTTAAGAGTAAG+AGG - tig0041342:6498-6517 Msa1461190:CDS 35.0%
GTGAAATTGTGGAATTTGGA+AGG + tig0041342:5986-6005 None:intergenic 35.0%
TGAGGTGAAATTGTGGAATT+TGG + tig0041342:5990-6009 None:intergenic 35.0%
ATTCCACAATTTCACCTCAA+CGG - tig0041342:5991-6010 Msa1461190:CDS 35.0%
CATGACAACATAATCTTCTG+TGG - tig0041342:6107-6126 Msa1461190:CDS 35.0%
! ATGGCCTTTGAATTTGAAGT+GGG + tig0041342:6194-6213 None:intergenic 35.0%
AAGGCCATGAAATCTAGTAA+TGG - tig0041342:6206-6225 Msa1461190:CDS 35.0%
GGAACCATTACTAGATTTCA+TGG + tig0041342:6213-6232 None:intergenic 35.0%
! ATAGCTAGCTTGAAGAGTAA+GGG - tig0041342:6233-6252 Msa1461190:CDS 35.0%
TTGAAGAGTAAGGGAAATGA+AGG - tig0041342:6242-6261 Msa1461190:CDS 35.0%
GGAAATGAAGGAGAAGTAAA+GGG - tig0041342:6254-6273 Msa1461190:CDS 35.0%
GAAGTCCAACAAAATCTAGA+TGG - tig0041342:6342-6361 Msa1461190:CDS 35.0%
AAGAGGTTGTTAAGAGTAAG+AGG - tig0041342:6498-6517 Msa1461190:CDS 35.0%
TTCTACTCAGATCAAGAAGC+AGG - tig0041342:5888-5907 Msa1461190:CDS 40.0%
TCTACTCAGATCAAGAAGCA+GGG - tig0041342:5889-5908 Msa1461190:CDS 40.0%
GGAATTTGGAAGGTCACAAA+GGG + tig0041342:5976-5995 None:intergenic 40.0%
TGGAATTTGGAAGGTCACAA+AGG + tig0041342:5977-5996 None:intergenic 40.0%
TTCCACAATTTCACCTCAAC+GGG - tig0041342:5992-6011 Msa1461190:CDS 40.0%
! ATGGTGATCCTTGCTGTAAT+TGG + tig0041342:6027-6046 None:intergenic 40.0%
CATGGCCTTTGAATTTGAAG+TGG + tig0041342:6195-6214 None:intergenic 40.0%
CTTACTCTTCAAGCTAGCTA+TGG + tig0041342:6234-6253 None:intergenic 40.0%
! CATAGCTAGCTTGAAGAGTA+AGG - tig0041342:6232-6251 Msa1461190:CDS 40.0%
GGGAAATGAAGGAGAAGTAA+AGG - tig0041342:6253-6272 Msa1461190:CDS 40.0%
! ACAACCTCTTTCCCATTTTC+CGG + tig0041342:6488-6507 None:intergenic 40.0%
TTCTACTCAGATCAAGAAGC+AGG - tig0041342:5888-5907 Msa1461190:CDS 40.0%
TCTACTCAGATCAAGAAGCA+GGG - tig0041342:5889-5908 Msa1461190:CDS 40.0%
GGAATTTGGAAGGTCACAAA+GGG + tig0041342:5976-5995 None:intergenic 40.0%
TGGAATTTGGAAGGTCACAA+AGG + tig0041342:5977-5996 None:intergenic 40.0%
TTCCACAATTTCACCTCAAC+GGG - tig0041342:5992-6011 Msa1461190:CDS 40.0%
! ATGGTGATCCTTGCTGTAAT+TGG + tig0041342:6027-6046 None:intergenic 40.0%
CATGGCCTTTGAATTTGAAG+TGG + tig0041342:6195-6214 None:intergenic 40.0%
CTTACTCTTCAAGCTAGCTA+TGG + tig0041342:6234-6253 None:intergenic 40.0%
! CATAGCTAGCTTGAAGAGTA+AGG - tig0041342:6232-6251 Msa1461190:CDS 40.0%
GGGAAATGAAGGAGAAGTAA+AGG - tig0041342:6253-6272 Msa1461190:CDS 40.0%
! ACAACCTCTTTCCCATTTTC+CGG + tig0041342:6488-6507 None:intergenic 40.0%
TCCACAATTTCACCTCAACG+GGG - tig0041342:5993-6012 Msa1461190:CDS 45.0%
TTGGAAAAATCACCCCGTTG+AGG + tig0041342:6008-6027 None:intergenic 45.0%
TGCTGGTGACTACACATCAA+TGG - tig0041342:6298-6317 Msa1461190:CDS 45.0%
GCTGGTGACTACACATCAAT+GGG - tig0041342:6299-6318 Msa1461190:CDS 45.0%
GTGACTACACATCAATGGGA+GGG - tig0041342:6303-6322 Msa1461190:CDS 45.0%
GTCACTAAGTTGCATCTCCT+TGG + tig0041342:6411-6430 None:intergenic 45.0%
GAAATAGACAGAGCAGAAGC+CGG - tig0041342:6435-6454 Msa1461190:CDS 45.0%
ATGTTTCCGACACCGGAAAA+TGG - tig0041342:6473-6492 Msa1461190:CDS 45.0%
TGTTTCCGACACCGGAAAAT+GGG - tig0041342:6474-6493 Msa1461190:CDS 45.0%
! TCTTTCCCATTTTCCGGTGT+CGG + tig0041342:6482-6501 None:intergenic 45.0%
TCCACAATTTCACCTCAACG+GGG - tig0041342:5993-6012 Msa1461190:CDS 45.0%
TTGGAAAAATCACCCCGTTG+AGG + tig0041342:6008-6027 None:intergenic 45.0%
TGCTGGTGACTACACATCAA+TGG - tig0041342:6298-6317 Msa1461190:CDS 45.0%
GCTGGTGACTACACATCAAT+GGG - tig0041342:6299-6318 Msa1461190:CDS 45.0%
GTGACTACACATCAATGGGA+GGG - tig0041342:6303-6322 Msa1461190:CDS 45.0%
GTCACTAAGTTGCATCTCCT+TGG + tig0041342:6411-6430 None:intergenic 45.0%
GAAATAGACAGAGCAGAAGC+CGG - tig0041342:6435-6454 Msa1461190:CDS 45.0%
ATGTTTCCGACACCGGAAAA+TGG - tig0041342:6473-6492 Msa1461190:CDS 45.0%
TGTTTCCGACACCGGAAAAT+GGG - tig0041342:6474-6493 Msa1461190:CDS 45.0%
! TCTTTCCCATTTTCCGGTGT+CGG + tig0041342:6482-6501 None:intergenic 45.0%
ACCCCGTTGAGGTGAAATTG+TGG + tig0041342:5997-6016 None:intergenic 50.0%
GGTGACTACACATCAATGGG+AGG - tig0041342:6302-6321 Msa1461190:CDS 50.0%
CTACACATCAATGGGAGGGA+AGG - tig0041342:6307-6326 Msa1461190:CDS 50.0%
TTGGAATGTCGTCGAGTTCG+AGG - tig0041342:6378-6397 Msa1461190:CDS 50.0%
GAGTTCGAGGATGTCTTCCA+AGG - tig0041342:6391-6410 Msa1461190:CDS 50.0%
! GGTGTCGGAAACATCGTCAT+CGG + tig0041342:6467-6486 None:intergenic 50.0%
GATGACGATGTTTCCGACAC+CGG - tig0041342:6466-6485 Msa1461190:CDS 50.0%
GACACCGGAAAATGGGAAAG+AGG - tig0041342:6481-6500 Msa1461190:CDS 50.0%
ACCCCGTTGAGGTGAAATTG+TGG + tig0041342:5997-6016 None:intergenic 50.0%
GGTGACTACACATCAATGGG+AGG - tig0041342:6302-6321 Msa1461190:CDS 50.0%
CTACACATCAATGGGAGGGA+AGG - tig0041342:6307-6326 Msa1461190:CDS 50.0%
TTGGAATGTCGTCGAGTTCG+AGG - tig0041342:6378-6397 Msa1461190:CDS 50.0%
GAGTTCGAGGATGTCTTCCA+AGG - tig0041342:6391-6410 Msa1461190:CDS 50.0%
! GGTGTCGGAAACATCGTCAT+CGG + tig0041342:6467-6486 None:intergenic 50.0%
GATGACGATGTTTCCGACAC+CGG - tig0041342:6466-6485 Msa1461190:CDS 50.0%
GACACCGGAAAATGGGAAAG+AGG - tig0041342:6481-6500 Msa1461190:CDS 50.0%
ATAGACAGAGCAGAAGCCGG+AGG - tig0041342:6438-6457 Msa1461190:CDS 55.0%
TAGACAGAGCAGAAGCCGGA+GGG - tig0041342:6439-6458 Msa1461190:CDS 55.0%
ATAGACAGAGCAGAAGCCGG+AGG - tig0041342:6438-6457 Msa1461190:CDS 55.0%
TAGACAGAGCAGAAGCCGGA+GGG - tig0041342:6439-6458 Msa1461190:CDS 55.0%
!! ACATCGTCATCGGCTCCCTC+CGG + tig0041342:6457-6476 None:intergenic 60.0%
!! ACATCGTCATCGGCTCCCTC+CGG + tig0041342:6457-6476 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0041342 gene 5873 6628 5873 ID=Msa1461190;Name=Msa1461190
tig0041342 mRNA 5873 6628 5873 ID=Msa1461190-mRNA-1;Parent=Msa1461190;Name=Msa1461190-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|251
tig0041342 exon 5873 6628 5873 ID=Msa1461190-mRNA-1:exon:23070;Parent=Msa1461190-mRNA-1
tig0041342 CDS 5873 6628 5873 ID=Msa1461190-mRNA-1:cds;Parent=Msa1461190-mRNA-1
Gene Sequence

>Msa1461190

ATGGTGGCTAAAGAATTCTACTCAGATCAAGAAGCAGGGAATGAAGAAAGTACTTTGCTACAAGATGAGTATGATGATTATGAAGAAGAAGAAACTTTATCCCTTTGTGACCTTCCAAATTCCACAATTTCACCTCAACGGGGTGATTTTTCCAATTACAGCAAGGATCACCATGATGATGATGATGATAACTTGTTTGAGTTTTTCAGTGAAGAATTCAACACTTCAACAGTTCATGACAACATAATCTTCTGTGGAAAACTCATTCCCTTCAAAGATCATCAACATGTTCCTCATAACCAAAAAAATTGTGCTAAACCCACTTCAAATTCAAAGGCCATGAAATCTAGTAATGGTTCCATAGCTAGCTTGAAGAGTAAGGGAAATGAAGGAGAAGTAAAGGGTAGTTTGAATGTAAAGAGTTTTGCTGGTGACTACACATCAATGGGAGGGAAGGTTTCACTTGTGAGAAGTCCAACAAAATCTAGATGGTTTTTGTTTATGTTTGGAATGTCGTCGAGTTCGAGGATGTCTTCCAAGGAGATGCAACTTAGTGACATTAGAAATAGACAGAGCAGAAGCCGGAGGGAGCCGATGACGATGTTTCCGACACCGGAAAATGGGAAAGAGGTTGTTAAGAGTAAGAGGAATAGGAATAGTAAAAGGATGTGGAAGATTTTGAAGTCAATTTCATTGGTTTTAGGATGCAGTAGTAGTAAACTTGCTAATGATGTAGTAAAGGCTGCTTTTGTGTGA

Protein sequence

>Msa1461190

MVAKEFYSDQEAGNEESTLLQDEYDDYEEEETLSLCDLPNSTISPQRGDFSNYSKDHHDDDDDNLFEFFSEEFNTSTVHDNIIFCGKLIPFKDHQHVPHNQKNCAKPTSNSKAMKSSNGSIASLKSKGNEGEVKGSLNVKSFAGDYTSMGGKVSLVRSPTKSRWFLFMFGMSSSSRMSSKEMQLSDIRNRQSRSRREPMTMFPTPENGKEVVKSKRNRNSKRMWKILKSISLVLGCSSSKLANDVVKAAFV*