Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000800 | Msa1443990 | 0.804351 | 2.252605e-49 | -8.615850e-47 |
| Msa0048380 | Msa1443990 | 0.806101 | 9.687095e-50 | -8.615850e-47 |
| Msa0051480 | Msa1443990 | 0.806453 | 8.164390e-50 | -8.615850e-47 |
| Msa0070120 | Msa1443990 | 0.840790 | 7.032604e-58 | -8.615850e-47 |
| Msa0073110 | Msa1443990 | 0.814003 | 1.922363e-51 | -8.615850e-47 |
| Msa0098380 | Msa1443990 | 0.806360 | 8.544852e-50 | -8.615850e-47 |
| Msa0107830 | Msa1443990 | 0.829367 | 5.355882e-55 | -8.615850e-47 |
| Msa0117650 | Msa1443990 | 0.828475 | 8.807328e-55 | -8.615850e-47 |
| Msa0126430 | Msa1443990 | 0.811676 | 6.213799e-51 | -8.615850e-47 |
| Msa0129290 | Msa1443990 | 0.831127 | 1.990794e-55 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1443990 | MtrunA17_Chr1g0200151 | 95.238 | 252 | 10 | 2 | 1 | 250 | 1 | 252 | 5.55e-152 | 423 |
Find 49 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATGTTGATGATCTTTGA+AGG | 0.488709 | tig0030644:+6883 | None:intergenic |
| AAGGCCATGAAATCTAGTAA+TGG | 0.615347 | tig0030644:-6818 | Msa1443990:CDS |
| ACATCGTCATCGGCTCCCTC+CGG | 0.586108 | tig0030644:+6570 | None:intergenic |
| ACATGTTGATGATCTTTGAA+GGG | 0.588773 | tig0030644:+6884 | None:intergenic |
| ACCCCGTTGAGGTGAAATTG+TGG | 0.594385 | tig0030644:+7030 | None:intergenic |
| ACTTGCTAATGATGTAGTAA+AGG | 0.515795 | tig0030644:-6435 | Msa1443990:CDS |
| AGGTTGTTAAGAGTAGGAAT+AGG | 0.451218 | tig0030644:-6523 | Msa1443990:CDS |
| AGTAGGAATAGGAATAGTAA+AGG | 0.434921 | tig0030644:-6512 | Msa1443990:CDS |
| ATAGACAGAGCAGAAGCCGG+AGG | 0.610980 | tig0030644:-6586 | Msa1443990:CDS |
| ATAGCTAGCTTGAAGAGTAA+GGG | 0.580739 | tig0030644:-6791 | Msa1443990:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTTATCATCATCATCATCA+TGG | + | tig0030644:6594-6613 | None:intergenic | 30.0% | |
| TAAACCCACTTCAAATTCAA+AGG | - | tig0030644:6735-6754 | Msa1443990:CDS | 30.0% | |
| AGTAGGAATAGGAATAGTAA+AGG | - | tig0030644:7060-7079 | Msa1443990:CDS | 30.0% | |
| GTAGGAATAGGAATAGTAAA+GGG | - | tig0030644:7061-7080 | Msa1443990:CDS | 30.0% | |
| AGTTATCATCATCATCATCA+TGG | + | tig0030644:6594-6613 | None:intergenic | 30.0% | |
| TAAACCCACTTCAAATTCAA+AGG | - | tig0030644:6735-6754 | Msa1443990:CDS | 30.0% | |
| AGTAGGAATAGGAATAGTAA+AGG | - | tig0030644:7060-7079 | Msa1443990:CDS | 30.0% | |
| GTAGGAATAGGAATAGTAAA+GGG | - | tig0030644:7061-7080 | Msa1443990:CDS | 30.0% | |
| GTGAAATTGTGGAATTTGGA+AGG | + | tig0030644:6534-6553 | None:intergenic | 35.0% | |
| TGAGGTGAAATTGTGGAATT+TGG | + | tig0030644:6538-6557 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0030644 | gene | 6421 | 7173 | 6421 | ID=Msa1443990;Name=Msa1443990 |
| tig0030644 | mRNA | 6421 | 7173 | 6421 | ID=Msa1443990-mRNA-1;Parent=Msa1443990;Name=Msa1443990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|250 |
| tig0030644 | exon | 6421 | 7173 | 6421 | ID=Msa1443990-mRNA-1:exon:14261;Parent=Msa1443990-mRNA-1 |
| tig0030644 | CDS | 6421 | 7173 | 6421 | ID=Msa1443990-mRNA-1:cds;Parent=Msa1443990-mRNA-1 |
| Gene Sequence |
>Msa1443990 ATGGTGGCTAAAGAATTCTACTCAGATCAAGAAGCAGGGAATGAAGAAAGTACTTTGCTACAAGATGAGTATGATGATTATGAAGAAGAAGAAACTTTATCCCTTTGTGACCTTCCAAATTCCACAATTTCACCTCAACGGGGTGATTTTTCCAATTACAGCAAGGATCACCATGATGATGATGATGATAACTTGTTTGAGTTTTTCAGTGAAGAATTCAACACTTCAACAGTTCATGACAACATAATCTTCTGTGGAAAACTCATTCCCTTCAAAGATCATCAACATGTTCCTCATAACCAAAAAAATTGTGCTAAACCCACTTCAAATTCAAAGGCCATGAAATCTAGTAATGGTTCCATAGCTAGCTTGAAGAGTAAGGGAAATGAAGGAGAAGTAAAGGGTAGTTTGAATGTAAAGAGTTTTGCTGGTGACTACACATCAATGGGAGGGAAGGTTTCACTTGTGAGAAGTCCAACAAAATCTAGATGGTTTTTGTTTATGTTTGGAATGTCGTCGAGTTCGAGGATGTCTTCCAAGGAGATGCAACTTAGTGACATTAGAAATAGACAGAGCAGAAGCCGGAGGGAGCCGATGACGATGTTTCCGACACCGGAAAATGGGAAAGAGGTTGTTAAGAGTAGGAATAGGAATAGTAAAGGGATGTGGAAGATTTTGAAGTCAATTTCATTGGTTTTAGGATGCAGTAGTAGTAAACTTGCTAATGATGTAGTAAAGGCTGCTTTTGTGTGA |
| Protein sequence |
>Msa1443990 MVAKEFYSDQEAGNEESTLLQDEYDDYEEEETLSLCDLPNSTISPQRGDFSNYSKDHHDDDDDNLFEFFSEEFNTSTVHDNIIFCGKLIPFKDHQHVPHNQKNCAKPTSNSKAMKSSNGSIASLKSKGNEGEVKGSLNVKSFAGDYTSMGGKVSLVRSPTKSRWFLFMFGMSSSSRMSSKEMQLSDIRNRQSRSRREPMTMFPTPENGKEVVKSRNRNSKGMWKILKSISLVLGCSSSKLANDVVKAAFV* |
