AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1443990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443990 MtrunA17_Chr1g0200151 95.238 252 10 2 1 250 1 252 5.55e-152 423
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTTCCGACACCGGAAAA+TGG 0.187842 tig0030644:-6551 Msa1443990:CDS
TGAGGTGAAATTGTGGAATT+TGG 0.256995 tig0030644:+7037 None:intergenic
TTCCACAATTTCACCTCAAC+GGG 0.312827 tig0030644:-7032 Msa1443990:CDS
ATGGTGATCCTTGCTGTAAT+TGG 0.353696 tig0030644:+7000 None:intergenic
GGAACCATTACTAGATTTCA+TGG 0.377121 tig0030644:+6814 None:intergenic
TGTTTCCGACACCGGAAAAT+GGG 0.386344 tig0030644:-6550 Msa1443990:CDS
CATAGCTAGCTTGAAGAGTA+AGG 0.421195 tig0030644:-6792 Msa1443990:CDS
AGTAGGAATAGGAATAGTAA+AGG 0.434921 tig0030644:-6512 Msa1443990:CDS
TTGAAGAGTAAGGGAAATGA+AGG 0.446303 tig0030644:-6782 Msa1443990:CDS
GGAAATGAAGGAGAAGTAAA+GGG 0.448337 tig0030644:-6770 Msa1443990:CDS
AGGTTGTTAAGAGTAGGAAT+AGG 0.451218 tig0030644:-6523 Msa1443990:CDS
TTGGAATGTCGTCGAGTTCG+AGG 0.453818 tig0030644:-6646 Msa1443990:CDS
TTCTACTCAGATCAAGAAGC+AGG 0.454222 tig0030644:-7136 Msa1443990:CDS
GTCACTAAGTTGCATCTCCT+TGG 0.456940 tig0030644:+6616 None:intergenic
GGGAAATGAAGGAGAAGTAA+AGG 0.459006 tig0030644:-6771 Msa1443990:CDS
TGCTGGTGACTACACATCAA+TGG 0.471580 tig0030644:-6726 Msa1443990:CDS
GCTGGTGACTACACATCAAT+GGG 0.479840 tig0030644:-6725 Msa1443990:CDS
GTAGGAATAGGAATAGTAAA+GGG 0.484219 tig0030644:-6511 Msa1443990:CDS
AACATGTTGATGATCTTTGA+AGG 0.488709 tig0030644:+6883 None:intergenic
CTTACTCTTCAAGCTAGCTA+TGG 0.509662 tig0030644:+6793 None:intergenic
ACTTGCTAATGATGTAGTAA+AGG 0.515795 tig0030644:-6435 Msa1443990:CDS
GAAGTCCAACAAAATCTAGA+TGG 0.518576 tig0030644:-6682 Msa1443990:CDS
ATAGGAATAGTAAAGGGATG+TGG 0.520278 tig0030644:-6505 Msa1443990:CDS
ATGGCCTTTGAATTTGAAGT+GGG 0.521547 tig0030644:+6833 None:intergenic
CATGGCCTTTGAATTTGAAG+TGG 0.528240 tig0030644:+6832 None:intergenic
GGAATTTGGAAGGTCACAAA+GGG 0.536647 tig0030644:+7051 None:intergenic
GAAATAGACAGAGCAGAAGC+CGG 0.555893 tig0030644:-6589 Msa1443990:CDS
TAAACCCACTTCAAATTCAA+AGG 0.577655 tig0030644:-6837 Msa1443990:CDS
GGAAAGAGGTTGTTAAGAGT+AGG 0.578489 tig0030644:-6529 Msa1443990:CDS
ATTCCACAATTTCACCTCAA+CGG 0.579273 tig0030644:-7033 Msa1443990:CDS
ATAGCTAGCTTGAAGAGTAA+GGG 0.580739 tig0030644:-6791 Msa1443990:CDS
GACACCGGAAAATGGGAAAG+AGG 0.585817 tig0030644:-6543 Msa1443990:CDS
ACATCGTCATCGGCTCCCTC+CGG 0.586108 tig0030644:+6570 None:intergenic
CATGACAACATAATCTTCTG+TGG 0.586506 tig0030644:-6917 Msa1443990:CDS
ACATGTTGATGATCTTTGAA+GGG 0.588773 tig0030644:+6884 None:intergenic
TGGAATTTGGAAGGTCACAA+AGG 0.590193 tig0030644:+7050 None:intergenic
GAGTTCGAGGATGTCTTCCA+AGG 0.590470 tig0030644:-6633 Msa1443990:CDS
GTGAAATTGTGGAATTTGGA+AGG 0.591726 tig0030644:+7041 None:intergenic
ACCCCGTTGAGGTGAAATTG+TGG 0.594385 tig0030644:+7030 None:intergenic
ATAGACAGAGCAGAAGCCGG+AGG 0.610980 tig0030644:-6586 Msa1443990:CDS
CTACACATCAATGGGAGGGA+AGG 0.612457 tig0030644:-6717 Msa1443990:CDS
AAGGCCATGAAATCTAGTAA+TGG 0.615347 tig0030644:-6818 Msa1443990:CDS
GGTGTCGGAAACATCGTCAT+CGG 0.617855 tig0030644:+6560 None:intergenic
TAGACAGAGCAGAAGCCGGA+GGG 0.622934 tig0030644:-6585 Msa1443990:CDS
GATGACGATGTTTCCGACAC+CGG 0.635603 tig0030644:-6558 Msa1443990:CDS
TCTACTCAGATCAAGAAGCA+GGG 0.638478 tig0030644:-7135 Msa1443990:CDS
GTGACTACACATCAATGGGA+GGG 0.664793 tig0030644:-6721 Msa1443990:CDS
GGTGACTACACATCAATGGG+AGG 0.668735 tig0030644:-6722 Msa1443990:CDS
TCCACAATTTCACCTCAACG+GGG 0.743576 tig0030644:-7031 Msa1443990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACCATCTAGATTTTGT+TGG + tig0030644:6898-6917 None:intergenic 25.0%
!!! AGATGGTTTTTGTTTATGTT+TGG - tig0030644:6907-6926 Msa1443990:CDS 25.0%
!!! GATTTTGAAGTCAATTTCAT+TGG - tig0030644:7092-7111 Msa1443990:CDS 25.0%
!!! AAGTCAATTTCATTGGTTTT+AGG - tig0030644:7099-7118 Msa1443990:CDS 25.0%
!! AAAAACCATCTAGATTTTGT+TGG + tig0030644:6898-6917 None:intergenic 25.0%
!!! AGATGGTTTTTGTTTATGTT+TGG - tig0030644:6907-6926 Msa1443990:CDS 25.0%
!!! GATTTTGAAGTCAATTTCAT+TGG - tig0030644:7092-7111 Msa1443990:CDS 25.0%
!!! AAGTCAATTTCATTGGTTTT+AGG - tig0030644:7099-7118 Msa1443990:CDS 25.0%
! TGATTTTTCCAATTACAGCA+AGG - tig0030644:6564-6583 Msa1443990:CDS 30.0%
AGTTATCATCATCATCATCA+TGG + tig0030644:6594-6613 None:intergenic 30.0%
!! ACATGTTGATGATCTTTGAA+GGG + tig0030644:6691-6710 None:intergenic 30.0%
!! AACATGTTGATGATCTTTGA+AGG + tig0030644:6692-6711 None:intergenic 30.0%
!!! GCACAATTTTTTTGGTTATG+AGG + tig0030644:6715-6734 None:intergenic 30.0%
!!! TGGGTTTAGCACAATTTTTT+TGG + tig0030644:6723-6742 None:intergenic 30.0%
TAAACCCACTTCAAATTCAA+AGG - tig0030644:6735-6754 Msa1443990:CDS 30.0%
! TTGAATGTAAAGAGTTTTGC+TGG - tig0030644:6829-6848 Msa1443990:CDS 30.0%
AGTAGGAATAGGAATAGTAA+AGG - tig0030644:7060-7079 Msa1443990:CDS 30.0%
GTAGGAATAGGAATAGTAAA+GGG - tig0030644:7061-7080 Msa1443990:CDS 30.0%
! ACTTGCTAATGATGTAGTAA+AGG - tig0030644:7137-7156 Msa1443990:CDS 30.0%
! TGATTTTTCCAATTACAGCA+AGG - tig0030644:6564-6583 Msa1443990:CDS 30.0%
AGTTATCATCATCATCATCA+TGG + tig0030644:6594-6613 None:intergenic 30.0%
!! ACATGTTGATGATCTTTGAA+GGG + tig0030644:6691-6710 None:intergenic 30.0%
!! AACATGTTGATGATCTTTGA+AGG + tig0030644:6692-6711 None:intergenic 30.0%
!!! GCACAATTTTTTTGGTTATG+AGG + tig0030644:6715-6734 None:intergenic 30.0%
!!! TGGGTTTAGCACAATTTTTT+TGG + tig0030644:6723-6742 None:intergenic 30.0%
TAAACCCACTTCAAATTCAA+AGG - tig0030644:6735-6754 Msa1443990:CDS 30.0%
! TTGAATGTAAAGAGTTTTGC+TGG - tig0030644:6829-6848 Msa1443990:CDS 30.0%
AGTAGGAATAGGAATAGTAA+AGG - tig0030644:7060-7079 Msa1443990:CDS 30.0%
GTAGGAATAGGAATAGTAAA+GGG - tig0030644:7061-7080 Msa1443990:CDS 30.0%
! ACTTGCTAATGATGTAGTAA+AGG - tig0030644:7137-7156 Msa1443990:CDS 30.0%
GTGAAATTGTGGAATTTGGA+AGG + tig0030644:6534-6553 None:intergenic 35.0%
TGAGGTGAAATTGTGGAATT+TGG + tig0030644:6538-6557 None:intergenic 35.0%
ATTCCACAATTTCACCTCAA+CGG - tig0030644:6539-6558 Msa1443990:CDS 35.0%
CATGACAACATAATCTTCTG+TGG - tig0030644:6655-6674 Msa1443990:CDS 35.0%
! ATGGCCTTTGAATTTGAAGT+GGG + tig0030644:6742-6761 None:intergenic 35.0%
AAGGCCATGAAATCTAGTAA+TGG - tig0030644:6754-6773 Msa1443990:CDS 35.0%
GGAACCATTACTAGATTTCA+TGG + tig0030644:6761-6780 None:intergenic 35.0%
! ATAGCTAGCTTGAAGAGTAA+GGG - tig0030644:6781-6800 Msa1443990:CDS 35.0%
TTGAAGAGTAAGGGAAATGA+AGG - tig0030644:6790-6809 Msa1443990:CDS 35.0%
GGAAATGAAGGAGAAGTAAA+GGG - tig0030644:6802-6821 Msa1443990:CDS 35.0%
GAAGTCCAACAAAATCTAGA+TGG - tig0030644:6890-6909 Msa1443990:CDS 35.0%
AGGTTGTTAAGAGTAGGAAT+AGG - tig0030644:7049-7068 Msa1443990:CDS 35.0%
ATAGGAATAGTAAAGGGATG+TGG - tig0030644:7067-7086 Msa1443990:CDS 35.0%
GTGAAATTGTGGAATTTGGA+AGG + tig0030644:6534-6553 None:intergenic 35.0%
TGAGGTGAAATTGTGGAATT+TGG + tig0030644:6538-6557 None:intergenic 35.0%
ATTCCACAATTTCACCTCAA+CGG - tig0030644:6539-6558 Msa1443990:CDS 35.0%
CATGACAACATAATCTTCTG+TGG - tig0030644:6655-6674 Msa1443990:CDS 35.0%
! ATGGCCTTTGAATTTGAAGT+GGG + tig0030644:6742-6761 None:intergenic 35.0%
AAGGCCATGAAATCTAGTAA+TGG - tig0030644:6754-6773 Msa1443990:CDS 35.0%
GGAACCATTACTAGATTTCA+TGG + tig0030644:6761-6780 None:intergenic 35.0%
! ATAGCTAGCTTGAAGAGTAA+GGG - tig0030644:6781-6800 Msa1443990:CDS 35.0%
TTGAAGAGTAAGGGAAATGA+AGG - tig0030644:6790-6809 Msa1443990:CDS 35.0%
GGAAATGAAGGAGAAGTAAA+GGG - tig0030644:6802-6821 Msa1443990:CDS 35.0%
GAAGTCCAACAAAATCTAGA+TGG - tig0030644:6890-6909 Msa1443990:CDS 35.0%
AGGTTGTTAAGAGTAGGAAT+AGG - tig0030644:7049-7068 Msa1443990:CDS 35.0%
ATAGGAATAGTAAAGGGATG+TGG - tig0030644:7067-7086 Msa1443990:CDS 35.0%
TTCTACTCAGATCAAGAAGC+AGG - tig0030644:6436-6455 Msa1443990:CDS 40.0%
TCTACTCAGATCAAGAAGCA+GGG - tig0030644:6437-6456 Msa1443990:CDS 40.0%
GGAATTTGGAAGGTCACAAA+GGG + tig0030644:6524-6543 None:intergenic 40.0%
TGGAATTTGGAAGGTCACAA+AGG + tig0030644:6525-6544 None:intergenic 40.0%
TTCCACAATTTCACCTCAAC+GGG - tig0030644:6540-6559 Msa1443990:CDS 40.0%
! ATGGTGATCCTTGCTGTAAT+TGG + tig0030644:6575-6594 None:intergenic 40.0%
CATGGCCTTTGAATTTGAAG+TGG + tig0030644:6743-6762 None:intergenic 40.0%
CTTACTCTTCAAGCTAGCTA+TGG + tig0030644:6782-6801 None:intergenic 40.0%
! CATAGCTAGCTTGAAGAGTA+AGG - tig0030644:6780-6799 Msa1443990:CDS 40.0%
GGGAAATGAAGGAGAAGTAA+AGG - tig0030644:6801-6820 Msa1443990:CDS 40.0%
! ACAACCTCTTTCCCATTTTC+CGG + tig0030644:7036-7055 None:intergenic 40.0%
GGAAAGAGGTTGTTAAGAGT+AGG - tig0030644:7043-7062 Msa1443990:CDS 40.0%
TTCTACTCAGATCAAGAAGC+AGG - tig0030644:6436-6455 Msa1443990:CDS 40.0%
TCTACTCAGATCAAGAAGCA+GGG - tig0030644:6437-6456 Msa1443990:CDS 40.0%
GGAATTTGGAAGGTCACAAA+GGG + tig0030644:6524-6543 None:intergenic 40.0%
TGGAATTTGGAAGGTCACAA+AGG + tig0030644:6525-6544 None:intergenic 40.0%
TTCCACAATTTCACCTCAAC+GGG - tig0030644:6540-6559 Msa1443990:CDS 40.0%
! ATGGTGATCCTTGCTGTAAT+TGG + tig0030644:6575-6594 None:intergenic 40.0%
CATGGCCTTTGAATTTGAAG+TGG + tig0030644:6743-6762 None:intergenic 40.0%
CTTACTCTTCAAGCTAGCTA+TGG + tig0030644:6782-6801 None:intergenic 40.0%
! CATAGCTAGCTTGAAGAGTA+AGG - tig0030644:6780-6799 Msa1443990:CDS 40.0%
GGGAAATGAAGGAGAAGTAA+AGG - tig0030644:6801-6820 Msa1443990:CDS 40.0%
! ACAACCTCTTTCCCATTTTC+CGG + tig0030644:7036-7055 None:intergenic 40.0%
GGAAAGAGGTTGTTAAGAGT+AGG - tig0030644:7043-7062 Msa1443990:CDS 40.0%
TCCACAATTTCACCTCAACG+GGG - tig0030644:6541-6560 Msa1443990:CDS 45.0%
TTGGAAAAATCACCCCGTTG+AGG + tig0030644:6556-6575 None:intergenic 45.0%
TGCTGGTGACTACACATCAA+TGG - tig0030644:6846-6865 Msa1443990:CDS 45.0%
GCTGGTGACTACACATCAAT+GGG - tig0030644:6847-6866 Msa1443990:CDS 45.0%
GTGACTACACATCAATGGGA+GGG - tig0030644:6851-6870 Msa1443990:CDS 45.0%
GTCACTAAGTTGCATCTCCT+TGG + tig0030644:6959-6978 None:intergenic 45.0%
GAAATAGACAGAGCAGAAGC+CGG - tig0030644:6983-7002 Msa1443990:CDS 45.0%
ATGTTTCCGACACCGGAAAA+TGG - tig0030644:7021-7040 Msa1443990:CDS 45.0%
TGTTTCCGACACCGGAAAAT+GGG - tig0030644:7022-7041 Msa1443990:CDS 45.0%
! TCTTTCCCATTTTCCGGTGT+CGG + tig0030644:7030-7049 None:intergenic 45.0%
TCCACAATTTCACCTCAACG+GGG - tig0030644:6541-6560 Msa1443990:CDS 45.0%
TTGGAAAAATCACCCCGTTG+AGG + tig0030644:6556-6575 None:intergenic 45.0%
TGCTGGTGACTACACATCAA+TGG - tig0030644:6846-6865 Msa1443990:CDS 45.0%
GCTGGTGACTACACATCAAT+GGG - tig0030644:6847-6866 Msa1443990:CDS 45.0%
GTGACTACACATCAATGGGA+GGG - tig0030644:6851-6870 Msa1443990:CDS 45.0%
GTCACTAAGTTGCATCTCCT+TGG + tig0030644:6959-6978 None:intergenic 45.0%
GAAATAGACAGAGCAGAAGC+CGG - tig0030644:6983-7002 Msa1443990:CDS 45.0%
ATGTTTCCGACACCGGAAAA+TGG - tig0030644:7021-7040 Msa1443990:CDS 45.0%
TGTTTCCGACACCGGAAAAT+GGG - tig0030644:7022-7041 Msa1443990:CDS 45.0%
! TCTTTCCCATTTTCCGGTGT+CGG + tig0030644:7030-7049 None:intergenic 45.0%
ACCCCGTTGAGGTGAAATTG+TGG + tig0030644:6545-6564 None:intergenic 50.0%
GGTGACTACACATCAATGGG+AGG - tig0030644:6850-6869 Msa1443990:CDS 50.0%
CTACACATCAATGGGAGGGA+AGG - tig0030644:6855-6874 Msa1443990:CDS 50.0%
TTGGAATGTCGTCGAGTTCG+AGG - tig0030644:6926-6945 Msa1443990:CDS 50.0%
GAGTTCGAGGATGTCTTCCA+AGG - tig0030644:6939-6958 Msa1443990:CDS 50.0%
! GGTGTCGGAAACATCGTCAT+CGG + tig0030644:7015-7034 None:intergenic 50.0%
GATGACGATGTTTCCGACAC+CGG - tig0030644:7014-7033 Msa1443990:CDS 50.0%
GACACCGGAAAATGGGAAAG+AGG - tig0030644:7029-7048 Msa1443990:CDS 50.0%
ACCCCGTTGAGGTGAAATTG+TGG + tig0030644:6545-6564 None:intergenic 50.0%
GGTGACTACACATCAATGGG+AGG - tig0030644:6850-6869 Msa1443990:CDS 50.0%
CTACACATCAATGGGAGGGA+AGG - tig0030644:6855-6874 Msa1443990:CDS 50.0%
TTGGAATGTCGTCGAGTTCG+AGG - tig0030644:6926-6945 Msa1443990:CDS 50.0%
GAGTTCGAGGATGTCTTCCA+AGG - tig0030644:6939-6958 Msa1443990:CDS 50.0%
! GGTGTCGGAAACATCGTCAT+CGG + tig0030644:7015-7034 None:intergenic 50.0%
GATGACGATGTTTCCGACAC+CGG - tig0030644:7014-7033 Msa1443990:CDS 50.0%
GACACCGGAAAATGGGAAAG+AGG - tig0030644:7029-7048 Msa1443990:CDS 50.0%
ATAGACAGAGCAGAAGCCGG+AGG - tig0030644:6986-7005 Msa1443990:CDS 55.0%
TAGACAGAGCAGAAGCCGGA+GGG - tig0030644:6987-7006 Msa1443990:CDS 55.0%
ATAGACAGAGCAGAAGCCGG+AGG - tig0030644:6986-7005 Msa1443990:CDS 55.0%
TAGACAGAGCAGAAGCCGGA+GGG - tig0030644:6987-7006 Msa1443990:CDS 55.0%
!! ACATCGTCATCGGCTCCCTC+CGG + tig0030644:7005-7024 None:intergenic 60.0%
!! ACATCGTCATCGGCTCCCTC+CGG + tig0030644:7005-7024 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0030644 gene 6421 7173 6421 ID=Msa1443990;Name=Msa1443990
tig0030644 mRNA 6421 7173 6421 ID=Msa1443990-mRNA-1;Parent=Msa1443990;Name=Msa1443990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|250
tig0030644 exon 6421 7173 6421 ID=Msa1443990-mRNA-1:exon:14261;Parent=Msa1443990-mRNA-1
tig0030644 CDS 6421 7173 6421 ID=Msa1443990-mRNA-1:cds;Parent=Msa1443990-mRNA-1
Gene Sequence

>Msa1443990

ATGGTGGCTAAAGAATTCTACTCAGATCAAGAAGCAGGGAATGAAGAAAGTACTTTGCTACAAGATGAGTATGATGATTATGAAGAAGAAGAAACTTTATCCCTTTGTGACCTTCCAAATTCCACAATTTCACCTCAACGGGGTGATTTTTCCAATTACAGCAAGGATCACCATGATGATGATGATGATAACTTGTTTGAGTTTTTCAGTGAAGAATTCAACACTTCAACAGTTCATGACAACATAATCTTCTGTGGAAAACTCATTCCCTTCAAAGATCATCAACATGTTCCTCATAACCAAAAAAATTGTGCTAAACCCACTTCAAATTCAAAGGCCATGAAATCTAGTAATGGTTCCATAGCTAGCTTGAAGAGTAAGGGAAATGAAGGAGAAGTAAAGGGTAGTTTGAATGTAAAGAGTTTTGCTGGTGACTACACATCAATGGGAGGGAAGGTTTCACTTGTGAGAAGTCCAACAAAATCTAGATGGTTTTTGTTTATGTTTGGAATGTCGTCGAGTTCGAGGATGTCTTCCAAGGAGATGCAACTTAGTGACATTAGAAATAGACAGAGCAGAAGCCGGAGGGAGCCGATGACGATGTTTCCGACACCGGAAAATGGGAAAGAGGTTGTTAAGAGTAGGAATAGGAATAGTAAAGGGATGTGGAAGATTTTGAAGTCAATTTCATTGGTTTTAGGATGCAGTAGTAGTAAACTTGCTAATGATGTAGTAAAGGCTGCTTTTGTGTGA

Protein sequence

>Msa1443990

MVAKEFYSDQEAGNEESTLLQDEYDDYEEEETLSLCDLPNSTISPQRGDFSNYSKDHHDDDDDNLFEFFSEEFNTSTVHDNIIFCGKLIPFKDHQHVPHNQKNCAKPTSNSKAMKSSNGSIASLKSKGNEGEVKGSLNVKSFAGDYTSMGGKVSLVRSPTKSRWFLFMFGMSSSSRMSSKEMQLSDIRNRQSRSRREPMTMFPTPENGKEVVKSRNRNSKGMWKILKSISLVLGCSSSKLANDVVKAAFV*