Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438470 | XP_003591967.1 | 95.238 | 252 | 10 | 2 | 1 | 250 | 1 | 252 | 6.03e-148 | 423 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438470 | G7I9M1 | 95.238 | 252 | 10 | 2 | 1 | 250 | 1 | 252 | 2.88e-148 | 423 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0070120 | Msa1438470 | 0.830960 | 2.187405e-55 | -8.615850e-47 |
| Msa0073110 | Msa1438470 | 0.804171 | 2.454753e-49 | -8.615850e-47 |
| Msa0107830 | Msa1438470 | 0.824323 | 8.589592e-54 | -8.615850e-47 |
| Msa0117650 | Msa1438470 | 0.825474 | 4.597212e-54 | -8.615850e-47 |
| Msa1377050 | Msa1438470 | 0.801695 | 7.973518e-49 | -8.615850e-47 |
| Msa1382740 | Msa1438470 | 0.800486 | 1.409278e-48 | -8.615850e-47 |
| Msa1387610 | Msa1438470 | 0.800362 | 1.493583e-48 | -8.615850e-47 |
| Msa1398120 | Msa1438470 | 0.823219 | 1.558583e-53 | -8.615850e-47 |
| Msa1438470 | Msa1443990 | 0.989113 | 9.554389e-177 | -8.615850e-47 |
| Msa1438470 | Msa1455480 | 0.804535 | 2.061542e-49 | -8.615850e-47 |
| Msa1438470 | Msa1459920 | 0.831299 | 1.805828e-55 | -8.615850e-47 |
| Msa1438470 | Msa1459930 | 0.802301 | 5.987563e-49 | -8.615850e-47 |
| Msa1438470 | Msa1461190 | 0.920086 | 1.996361e-87 | -8.615850e-47 |
| Msa0603300 | Msa1438470 | 0.802818 | 4.683773e-49 | -8.615850e-47 |
| Msa0603310 | Msa1438470 | 0.838823 | 2.287206e-57 | -8.615850e-47 |
| Msa0611060 | Msa1438470 | 0.804869 | 1.755646e-49 | -8.615850e-47 |
| Msa0614960 | Msa1438470 | 0.834541 | 2.823288e-56 | -8.615850e-47 |
| Msa0615200 | Msa1438470 | 0.811438 | 7.000298e-51 | -8.615850e-47 |
| Msa0628200 | Msa1438470 | 0.811904 | 5.543690e-51 | -8.615850e-47 |
| Msa0631130 | Msa1438470 | 0.814514 | 1.482293e-51 | -8.615850e-47 |
| Msa0637590 | Msa1438470 | 0.822897 | 1.852439e-53 | -8.615850e-47 |
| Msa0637700 | Msa1438470 | 0.805222 | 1.481750e-49 | -8.615850e-47 |
| Msa0644340 | Msa1438470 | 0.818021 | 2.436016e-52 | -8.615850e-47 |
| Msa0652590 | Msa1438470 | 0.840121 | 1.051970e-57 | -8.615850e-47 |
| Msa0652600 | Msa1438470 | 0.837618 | 4.673333e-57 | -8.615850e-47 |
| Msa0655370 | Msa1438470 | 0.802583 | 5.238114e-49 | -8.615850e-47 |
| Msa0657340 | Msa1438470 | 0.816213 | 6.210672e-52 | -8.615850e-47 |
| Msa0669350 | Msa1438470 | 0.811034 | 8.564253e-51 | -8.615850e-47 |
| Msa0675590 | Msa1438470 | 0.800123 | 1.670678e-48 | -8.615850e-47 |
| Msa0682110 | Msa1438470 | 0.804617 | 1.982466e-49 | -8.615850e-47 |
| Msa0682160 | Msa1438470 | 0.810726 | 9.990129e-51 | -8.615850e-47 |
| Msa0694320 | Msa1438470 | 0.819034 | 1.435132e-52 | -8.615850e-47 |
| Msa0699170 | Msa1438470 | 0.846046 | 2.777433e-59 | -8.615850e-47 |
| Msa0699800 | Msa1438470 | 0.806830 | 6.799436e-50 | -8.615850e-47 |
| Msa0701430 | Msa1438470 | 0.816942 | 4.264240e-52 | -8.615850e-47 |
| Msa0716620 | Msa1438470 | 0.837403 | 5.306653e-57 | -8.615850e-47 |
| Msa0728230 | Msa1438470 | 0.802056 | 6.722190e-49 | -8.615850e-47 |
| Msa0731580 | Msa1438470 | 0.806940 | 6.444819e-50 | -8.615850e-47 |
| Msa0736560 | Msa1438470 | 0.835019 | 2.140791e-56 | -8.615850e-47 |
| Msa0744550 | Msa1438470 | 0.825735 | 3.986532e-54 | -8.615850e-47 |
| Msa0744560 | Msa1438470 | 0.824765 | 6.758318e-54 | -8.615850e-47 |
| Msa0744710 | Msa1438470 | 0.839188 | 1.840041e-57 | -8.615850e-47 |
| Msa0750830 | Msa1438470 | 0.804181 | 2.443462e-49 | -8.615850e-47 |
| Msa0772350 | Msa1438470 | 0.814762 | 1.306263e-51 | -8.615850e-47 |
| Msa0793270 | Msa1438470 | 0.801989 | 6.938999e-49 | -8.615850e-47 |
| Msa0795320 | Msa1438470 | 0.836303 | 1.012476e-56 | -8.615850e-47 |
| Msa0812050 | Msa1438470 | 0.806986 | 6.301633e-50 | -8.615850e-47 |
| Msa0826240 | Msa1438470 | 0.840897 | 6.592008e-58 | -8.615850e-47 |
| Msa0826360 | Msa1438470 | 0.809098 | 2.238525e-50 | -8.615850e-47 |
| Msa0837550 | Msa1438470 | 0.805849 | 1.094643e-49 | -8.615850e-47 |
| Msa0126430 | Msa1438470 | 0.806050 | 9.926679e-50 | -8.615850e-47 |
| Msa0129290 | Msa1438470 | 0.822102 | 2.834266e-53 | -8.615850e-47 |
| Msa0143640 | Msa1438470 | 0.801834 | 7.469305e-49 | -8.615850e-47 |
| Msa0150640 | Msa1438470 | 0.802506 | 5.432000e-49 | -8.615850e-47 |
| Msa0161230 | Msa1438470 | 0.805034 | 1.621746e-49 | -8.615850e-47 |
| Msa0185040 | Msa1438470 | 0.840655 | 7.627704e-58 | -8.615850e-47 |
| Msa0186240 | Msa1438470 | 0.838134 | 3.443158e-57 | -8.615850e-47 |
| Msa1239190 | Msa1438470 | 0.810652 | 1.036259e-50 | -8.615850e-47 |
| Msa1239210 | Msa1438470 | 0.819299 | 1.249272e-52 | -8.615850e-47 |
| Msa1239940 | Msa1438470 | 0.801237 | 9.897963e-49 | -8.615850e-47 |
| Msa1254870 | Msa1438470 | 0.846269 | 2.415624e-59 | -8.615850e-47 |
| Msa1269200 | Msa1438470 | 0.814757 | 1.309109e-51 | -8.615850e-47 |
| Msa1299480 | Msa1438470 | 0.829331 | 5.463920e-55 | -8.615850e-47 |
| Msa1320160 | Msa1438470 | 0.800728 | 1.257558e-48 | -8.615850e-47 |
| Msa1328430 | Msa1438470 | 0.847963 | 8.293491e-60 | -8.615850e-47 |
| Msa0378320 | Msa1438470 | 0.813864 | 2.063051e-51 | -8.615850e-47 |
| Msa0383900 | Msa1438470 | 0.835751 | 1.398201e-56 | -8.615850e-47 |
| Msa0390200 | Msa1438470 | 0.822467 | 2.332474e-53 | -8.615850e-47 |
| Msa0393100 | Msa1438470 | 0.820587 | 6.339196e-53 | -8.615850e-47 |
| Msa0400010 | Msa1438470 | 0.816285 | 5.982213e-52 | -8.615850e-47 |
| Msa0405200 | Msa1438470 | 0.802999 | 4.297009e-49 | -8.615850e-47 |
| Msa0421080 | Msa1438470 | 0.810609 | 1.058461e-50 | -8.615850e-47 |
| Msa0427660 | Msa1438470 | 0.824494 | 7.832025e-54 | -8.615850e-47 |
| Msa0434300 | Msa1438470 | 0.835941 | 1.251181e-56 | -8.615850e-47 |
| Msa0434400 | Msa1438470 | 0.832688 | 8.195691e-56 | -8.615850e-47 |
| Msa0441750 | Msa1438470 | 0.806631 | 7.491110e-50 | -8.615850e-47 |
| Msa0449500 | Msa1438470 | 0.811181 | 7.959509e-51 | -8.615850e-47 |
| Msa0459930 | Msa1438470 | 0.802005 | 6.887843e-49 | -8.615850e-47 |
| Msa0460600 | Msa1438470 | 0.812047 | 5.159189e-51 | -8.615850e-47 |
| Msa0478080 | Msa1438470 | 0.806104 | 9.670502e-50 | -8.615850e-47 |
| Msa0480540 | Msa1438470 | 0.823103 | 1.658121e-53 | -8.615850e-47 |
| Msa0480550 | Msa1438470 | 0.825453 | 4.648212e-54 | -8.615850e-47 |
| Msa1001120 | Msa1438470 | 0.824020 | 1.011785e-53 | -8.615850e-47 |
| Msa1003920 | Msa1438470 | 0.801448 | 8.960618e-49 | -8.615850e-47 |
| Msa1027340 | Msa1438470 | 0.805917 | 1.059129e-49 | -8.615850e-47 |
| Msa1027400 | Msa1438470 | 0.805690 | 1.181951e-49 | -8.615850e-47 |
| Msa1030210 | Msa1438470 | 0.846114 | 2.661496e-59 | -8.615850e-47 |
| Msa1035050 | Msa1438470 | 0.832688 | 8.194730e-56 | -8.615850e-47 |
| Msa1041250 | Msa1438470 | 0.800233 | 1.586493e-48 | -8.615850e-47 |
| Msa1061840 | Msa1438470 | 0.812275 | 4.600500e-51 | -8.615850e-47 |
| Msa1062060 | Msa1438470 | 0.824193 | 9.214466e-54 | -8.615850e-47 |
| Msa1069250 | Msa1438470 | 0.828444 | 8.962115e-55 | -8.615850e-47 |
| Msa1075090 | Msa1438470 | 0.837656 | 4.569884e-57 | -8.615850e-47 |
| Msa1077970 | Msa1438470 | 0.823568 | 1.291508e-53 | -8.615850e-47 |
| Msa1102210 | Msa1438470 | 0.814666 | 1.371476e-51 | -8.615850e-47 |
| Msa0242720 | Msa1438470 | 0.844424 | 7.627191e-59 | -8.615850e-47 |
| Msa0246140 | Msa1438470 | 0.813254 | 2.809809e-51 | -8.615850e-47 |
| Msa0265650 | Msa1438470 | 0.834811 | 2.415625e-56 | -8.615850e-47 |
| Msa0267700 | Msa1438470 | 0.815736 | 7.934369e-52 | -8.615850e-47 |
| Msa0271700 | Msa1438470 | 0.821303 | 4.338299e-53 | -8.615850e-47 |
| Msa0271720 | Msa1438470 | 0.820027 | 8.521907e-53 | -8.615850e-47 |
| Msa0275730 | Msa1438470 | 0.807916 | 3.999999e-50 | -8.615850e-47 |
| Msa0281000 | Msa1438470 | 0.803330 | 3.670825e-49 | -8.615850e-47 |
| Msa0303900 | Msa1438470 | 0.801878 | 7.315371e-49 | -8.615850e-47 |
| Msa0316230 | Msa1438470 | 0.812835 | 3.471550e-51 | -8.615850e-47 |
| Msa0319680 | Msa1438470 | 0.811676 | 6.213674e-51 | -8.615850e-47 |
| Msa0320170 | Msa1438470 | 0.833180 | 6.185008e-56 | -8.615850e-47 |
| Msa0324810 | Msa1438470 | 0.820128 | 8.079355e-53 | -8.615850e-47 |
| Msa0333770 | Msa1438470 | 0.800188 | 1.620219e-48 | -8.615850e-47 |
| Msa0346170 | Msa1438470 | 0.803153 | 3.992732e-49 | -8.615850e-47 |
| Msa0353010 | Msa1438470 | 0.828782 | 7.424701e-55 | -8.615850e-47 |
| Msa1107220 | Msa1438470 | 0.821268 | 4.419182e-53 | -8.615850e-47 |
| Msa1107250 | Msa1438470 | 0.805714 | 1.168178e-49 | -8.615850e-47 |
| Msa1110900 | Msa1438470 | 0.841203 | 5.480378e-58 | -8.615850e-47 |
| Msa1114670 | Msa1438470 | 0.805154 | 1.530908e-49 | -8.615850e-47 |
| Msa1115980 | Msa1438470 | 0.813175 | 2.923524e-51 | -8.615850e-47 |
| Msa1120580 | Msa1438470 | 0.835707 | 1.434477e-56 | -8.615850e-47 |
| Msa1136630 | Msa1438470 | 0.817000 | 4.137142e-52 | -8.615850e-47 |
| Msa1137750 | Msa1438470 | 0.810391 | 1.179974e-50 | -8.615850e-47 |
| Msa1150760 | Msa1438470 | 0.815718 | 8.007134e-52 | -8.615850e-47 |
| Msa1151070 | Msa1438470 | 0.805804 | 1.118541e-49 | -8.615850e-47 |
| Msa1154570 | Msa1438470 | 0.807531 | 4.831240e-50 | -8.615850e-47 |
| Msa1157110 | Msa1438470 | 0.848032 | 7.935391e-60 | -8.615850e-47 |
| Msa1162430 | Msa1438470 | 0.853939 | 1.714670e-61 | -8.615850e-47 |
| Msa1174280 | Msa1438470 | 0.844903 | 5.667211e-59 | -8.615850e-47 |
| Msa1185270 | Msa1438470 | 0.822712 | 2.046129e-53 | -8.615850e-47 |
| Msa1202110 | Msa1438470 | 0.846362 | 2.278642e-59 | -8.615850e-47 |
| Msa1211610 | Msa1438470 | 0.834751 | 2.500592e-56 | -8.615850e-47 |
| Msa1223990 | Msa1438470 | 0.808458 | 3.067281e-50 | -8.615850e-47 |
| Msa0488580 | Msa1438470 | 0.809515 | 1.821549e-50 | -8.615850e-47 |
| Msa0493870 | Msa1438470 | 0.806090 | 9.739334e-50 | -8.615850e-47 |
| Msa0497680 | Msa1438470 | 0.816144 | 6.433404e-52 | -8.615850e-47 |
| Msa0497860 | Msa1438470 | 0.820595 | 6.311378e-53 | -8.615850e-47 |
| Msa0518490 | Msa1438470 | 0.814094 | 1.835393e-51 | -8.615850e-47 |
| Msa0522140 | Msa1438470 | 0.840556 | 8.098717e-58 | -8.615850e-47 |
| Msa0522170 | Msa1438470 | 0.838794 | 2.327183e-57 | -8.615850e-47 |
| Msa0534610 | Msa1438470 | 0.810988 | 8.766879e-51 | -8.615850e-47 |
| Msa0548420 | Msa1438470 | 0.810062 | 1.389374e-50 | -8.615850e-47 |
| Msa0548790 | Msa1438470 | 0.812360 | 4.408414e-51 | -8.615850e-47 |
| Msa0556270 | Msa1438470 | 0.813580 | 2.382066e-51 | -8.615850e-47 |
| Msa0557240 | Msa1438470 | 0.828997 | 6.587189e-55 | -8.615850e-47 |
| Msa0557250 | Msa1438470 | 0.832800 | 7.686536e-56 | -8.615850e-47 |
| Msa0561110 | Msa1438470 | 0.804855 | 1.767615e-49 | -8.615850e-47 |
| Msa0561830 | Msa1438470 | 0.804399 | 2.201335e-49 | -8.615850e-47 |
| Msa0565180 | Msa1438470 | 0.820649 | 6.134250e-53 | -8.615850e-47 |
| Msa0588040 | Msa1438470 | 0.814349 | 1.612127e-51 | -8.615850e-47 |
| Msa0588730 | Msa1438470 | 0.810946 | 8.950663e-51 | -8.615850e-47 |
| Msa0590810 | Msa1438470 | 0.816997 | 4.144103e-52 | -8.615850e-47 |
| Msa0597820 | Msa1438470 | 0.804352 | 2.251278e-49 | -8.615850e-47 |
| Msa0855260 | Msa1438470 | 0.805458 | 1.322131e-49 | -8.615850e-47 |
| Msa0865810 | Msa1438470 | 0.802831 | 4.654225e-49 | -8.615850e-47 |
| Msa0865820 | Msa1438470 | 0.812761 | 3.603076e-51 | -8.615850e-47 |
| Msa0865830 | Msa1438470 | 0.826860 | 2.151347e-54 | -8.615850e-47 |
| Msa0874640 | Msa1438470 | 0.810725 | 9.994623e-51 | -8.615850e-47 |
| Msa0874750 | Msa1438470 | 0.833926 | 4.028427e-56 | -8.615850e-47 |
| Msa0901270 | Msa1438470 | 0.806557 | 7.764017e-50 | -8.615850e-47 |
| Msa0925940 | Msa1438470 | 0.821663 | 3.581382e-53 | -8.615850e-47 |
| Msa0945330 | Msa1438470 | 0.821005 | 5.080608e-53 | -8.615850e-47 |
| Msa0968990 | Msa1438470 | 0.810632 | 1.046364e-50 | -8.615850e-47 |
| Msa0976400 | Msa1438470 | 0.817634 | 2.978828e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438470 | MtrunA17_Chr1g0200151 | 95.238 | 252 | 10 | 2 | 1 | 250 | 1 | 252 | 5.55e-152 | 423 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTTTCCGACACCGGAAAA+TGG | 0.187842 | tig0028560:-26055 | Msa1438470:CDS |
| TGAGGTGAAATTGTGGAATT+TGG | 0.256995 | tig0028560:+26541 | None:intergenic |
| TTCCACAATTTCACCTCAAC+GGG | 0.312827 | tig0028560:-26536 | Msa1438470:CDS |
| ATGGTGATCCTTGCTGTAAT+TGG | 0.353696 | tig0028560:+26504 | None:intergenic |
| GGAACCATTACTAGATTTCA+TGG | 0.377121 | tig0028560:+26318 | None:intergenic |
| TGTTTCCGACACCGGAAAAT+GGG | 0.386344 | tig0028560:-26054 | Msa1438470:CDS |
| CATAGCTAGCTTGAAGAGTA+AGG | 0.421195 | tig0028560:-26296 | Msa1438470:CDS |
| AGTAGGAATAGGAATAGTAA+AGG | 0.434921 | tig0028560:-26016 | Msa1438470:CDS |
| TTGAAGAGTAAGGGAAATGA+AGG | 0.446303 | tig0028560:-26286 | Msa1438470:CDS |
| GGAAATGAAGGAGAAGTAAA+GGG | 0.448337 | tig0028560:-26274 | Msa1438470:CDS |
| AGGTTGTTAAGAGTAGGAAT+AGG | 0.451218 | tig0028560:-26027 | Msa1438470:CDS |
| TTGGAATGTCGTCGAGTTCG+AGG | 0.453818 | tig0028560:-26150 | Msa1438470:CDS |
| TTCTACTCAGATCAAGAAGC+AGG | 0.454222 | tig0028560:-26640 | Msa1438470:CDS |
| GTCACTAAGTTGCATCTCCT+TGG | 0.456940 | tig0028560:+26120 | None:intergenic |
| GGGAAATGAAGGAGAAGTAA+AGG | 0.459006 | tig0028560:-26275 | Msa1438470:CDS |
| TGCTGGTGACTACACATCAA+TGG | 0.471580 | tig0028560:-26230 | Msa1438470:CDS |
| GCTGGTGACTACACATCAAT+GGG | 0.479840 | tig0028560:-26229 | Msa1438470:CDS |
| GTAGGAATAGGAATAGTAAA+GGG | 0.484219 | tig0028560:-26015 | Msa1438470:CDS |
| AACATGTTGATGATCTTTGA+AGG | 0.488709 | tig0028560:+26387 | None:intergenic |
| CTTACTCTTCAAGCTAGCTA+TGG | 0.509662 | tig0028560:+26297 | None:intergenic |
| ACTTGCTAATGATGTAGTAA+AGG | 0.515795 | tig0028560:-25939 | Msa1438470:CDS |
| GAAGTCCAACAAAATCTAGA+TGG | 0.518576 | tig0028560:-26186 | Msa1438470:CDS |
| ATAGGAATAGTAAAGGGATG+TGG | 0.520278 | tig0028560:-26009 | Msa1438470:CDS |
| ATGGCCTTTGAATTTGAAGT+GGG | 0.521547 | tig0028560:+26337 | None:intergenic |
| CATGGCCTTTGAATTTGAAG+TGG | 0.528240 | tig0028560:+26336 | None:intergenic |
| GGAATTTGGAAGGTCACAAA+GGG | 0.536647 | tig0028560:+26555 | None:intergenic |
| GAAATAGACAGAGCAGAAGC+CGG | 0.555893 | tig0028560:-26093 | Msa1438470:CDS |
| TAAACCCACTTCAAATTCAA+AGG | 0.577655 | tig0028560:-26341 | Msa1438470:CDS |
| GGAAAGAGGTTGTTAAGAGT+AGG | 0.578489 | tig0028560:-26033 | Msa1438470:CDS |
| ATTCCACAATTTCACCTCAA+CGG | 0.579273 | tig0028560:-26537 | Msa1438470:CDS |
| ATAGCTAGCTTGAAGAGTAA+GGG | 0.580739 | tig0028560:-26295 | Msa1438470:CDS |
| GACACCGGAAAATGGGAAAG+AGG | 0.585817 | tig0028560:-26047 | Msa1438470:CDS |
| ACATCGTCATCGGCTCCCTC+CGG | 0.586108 | tig0028560:+26074 | None:intergenic |
| CATGACAACATAATCTTCTG+TGG | 0.586506 | tig0028560:-26421 | Msa1438470:CDS |
| ACATGTTGATGATCTTTGAA+GGG | 0.588773 | tig0028560:+26388 | None:intergenic |
| TGGAATTTGGAAGGTCACAA+AGG | 0.590193 | tig0028560:+26554 | None:intergenic |
| GAGTTCGAGGATGTCTTCCA+AGG | 0.590470 | tig0028560:-26137 | Msa1438470:CDS |
| GTGAAATTGTGGAATTTGGA+AGG | 0.591726 | tig0028560:+26545 | None:intergenic |
| ACCCCGTTGAGGTGAAATTG+TGG | 0.594385 | tig0028560:+26534 | None:intergenic |
| ATAGACAGAGCAGAAGCCGG+AGG | 0.610980 | tig0028560:-26090 | Msa1438470:CDS |
| CTACACATCAATGGGAGGGA+AGG | 0.612457 | tig0028560:-26221 | Msa1438470:CDS |
| AAGGCCATGAAATCTAGTAA+TGG | 0.615347 | tig0028560:-26322 | Msa1438470:CDS |
| GGTGTCGGAAACATCGTCAT+CGG | 0.617855 | tig0028560:+26064 | None:intergenic |
| TAGACAGAGCAGAAGCCGGA+GGG | 0.622934 | tig0028560:-26089 | Msa1438470:CDS |
| GATGACGATGTTTCCGACAC+CGG | 0.635603 | tig0028560:-26062 | Msa1438470:CDS |
| TCTACTCAGATCAAGAAGCA+GGG | 0.638478 | tig0028560:-26639 | Msa1438470:CDS |
| GTGACTACACATCAATGGGA+GGG | 0.664793 | tig0028560:-26225 | Msa1438470:CDS |
| GGTGACTACACATCAATGGG+AGG | 0.668735 | tig0028560:-26226 | Msa1438470:CDS |
| TCCACAATTTCACCTCAACG+GGG | 0.743576 | tig0028560:-26535 | Msa1438470:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACCATCTAGATTTTGT+TGG | + | tig0028560:26402-26421 | None:intergenic | 25.0% |
| !!! | AGATGGTTTTTGTTTATGTT+TGG | - | tig0028560:26411-26430 | Msa1438470:CDS | 25.0% |
| !!! | GATTTTGAAGTCAATTTCAT+TGG | - | tig0028560:26596-26615 | Msa1438470:CDS | 25.0% |
| !!! | AAGTCAATTTCATTGGTTTT+AGG | - | tig0028560:26603-26622 | Msa1438470:CDS | 25.0% |
| !! | AAAAACCATCTAGATTTTGT+TGG | + | tig0028560:26402-26421 | None:intergenic | 25.0% |
| !!! | AGATGGTTTTTGTTTATGTT+TGG | - | tig0028560:26411-26430 | Msa1438470:CDS | 25.0% |
| !!! | GATTTTGAAGTCAATTTCAT+TGG | - | tig0028560:26596-26615 | Msa1438470:CDS | 25.0% |
| !!! | AAGTCAATTTCATTGGTTTT+AGG | - | tig0028560:26603-26622 | Msa1438470:CDS | 25.0% |
| ! | TGATTTTTCCAATTACAGCA+AGG | - | tig0028560:26068-26087 | Msa1438470:CDS | 30.0% |
| AGTTATCATCATCATCATCA+TGG | + | tig0028560:26098-26117 | None:intergenic | 30.0% | |
| !! | ACATGTTGATGATCTTTGAA+GGG | + | tig0028560:26195-26214 | None:intergenic | 30.0% |
| !! | AACATGTTGATGATCTTTGA+AGG | + | tig0028560:26196-26215 | None:intergenic | 30.0% |
| !!! | GCACAATTTTTTTGGTTATG+AGG | + | tig0028560:26219-26238 | None:intergenic | 30.0% |
| !!! | TGGGTTTAGCACAATTTTTT+TGG | + | tig0028560:26227-26246 | None:intergenic | 30.0% |
| TAAACCCACTTCAAATTCAA+AGG | - | tig0028560:26239-26258 | Msa1438470:CDS | 30.0% | |
| ! | TTGAATGTAAAGAGTTTTGC+TGG | - | tig0028560:26333-26352 | Msa1438470:CDS | 30.0% |
| AGTAGGAATAGGAATAGTAA+AGG | - | tig0028560:26564-26583 | Msa1438470:CDS | 30.0% | |
| GTAGGAATAGGAATAGTAAA+GGG | - | tig0028560:26565-26584 | Msa1438470:CDS | 30.0% | |
| ! | ACTTGCTAATGATGTAGTAA+AGG | - | tig0028560:26641-26660 | Msa1438470:CDS | 30.0% |
| ! | TGATTTTTCCAATTACAGCA+AGG | - | tig0028560:26068-26087 | Msa1438470:CDS | 30.0% |
| AGTTATCATCATCATCATCA+TGG | + | tig0028560:26098-26117 | None:intergenic | 30.0% | |
| !! | ACATGTTGATGATCTTTGAA+GGG | + | tig0028560:26195-26214 | None:intergenic | 30.0% |
| !! | AACATGTTGATGATCTTTGA+AGG | + | tig0028560:26196-26215 | None:intergenic | 30.0% |
| !!! | GCACAATTTTTTTGGTTATG+AGG | + | tig0028560:26219-26238 | None:intergenic | 30.0% |
| !!! | TGGGTTTAGCACAATTTTTT+TGG | + | tig0028560:26227-26246 | None:intergenic | 30.0% |
| TAAACCCACTTCAAATTCAA+AGG | - | tig0028560:26239-26258 | Msa1438470:CDS | 30.0% | |
| ! | TTGAATGTAAAGAGTTTTGC+TGG | - | tig0028560:26333-26352 | Msa1438470:CDS | 30.0% |
| AGTAGGAATAGGAATAGTAA+AGG | - | tig0028560:26564-26583 | Msa1438470:CDS | 30.0% | |
| GTAGGAATAGGAATAGTAAA+GGG | - | tig0028560:26565-26584 | Msa1438470:CDS | 30.0% | |
| ! | ACTTGCTAATGATGTAGTAA+AGG | - | tig0028560:26641-26660 | Msa1438470:CDS | 30.0% |
| GTGAAATTGTGGAATTTGGA+AGG | + | tig0028560:26038-26057 | None:intergenic | 35.0% | |
| TGAGGTGAAATTGTGGAATT+TGG | + | tig0028560:26042-26061 | None:intergenic | 35.0% | |
| ATTCCACAATTTCACCTCAA+CGG | - | tig0028560:26043-26062 | Msa1438470:CDS | 35.0% | |
| CATGACAACATAATCTTCTG+TGG | - | tig0028560:26159-26178 | Msa1438470:CDS | 35.0% | |
| ! | ATGGCCTTTGAATTTGAAGT+GGG | + | tig0028560:26246-26265 | None:intergenic | 35.0% |
| AAGGCCATGAAATCTAGTAA+TGG | - | tig0028560:26258-26277 | Msa1438470:CDS | 35.0% | |
| GGAACCATTACTAGATTTCA+TGG | + | tig0028560:26265-26284 | None:intergenic | 35.0% | |
| ! | ATAGCTAGCTTGAAGAGTAA+GGG | - | tig0028560:26285-26304 | Msa1438470:CDS | 35.0% |
| TTGAAGAGTAAGGGAAATGA+AGG | - | tig0028560:26294-26313 | Msa1438470:CDS | 35.0% | |
| GGAAATGAAGGAGAAGTAAA+GGG | - | tig0028560:26306-26325 | Msa1438470:CDS | 35.0% | |
| GAAGTCCAACAAAATCTAGA+TGG | - | tig0028560:26394-26413 | Msa1438470:CDS | 35.0% | |
| AGGTTGTTAAGAGTAGGAAT+AGG | - | tig0028560:26553-26572 | Msa1438470:CDS | 35.0% | |
| ATAGGAATAGTAAAGGGATG+TGG | - | tig0028560:26571-26590 | Msa1438470:CDS | 35.0% | |
| GTGAAATTGTGGAATTTGGA+AGG | + | tig0028560:26038-26057 | None:intergenic | 35.0% | |
| TGAGGTGAAATTGTGGAATT+TGG | + | tig0028560:26042-26061 | None:intergenic | 35.0% | |
| ATTCCACAATTTCACCTCAA+CGG | - | tig0028560:26043-26062 | Msa1438470:CDS | 35.0% | |
| CATGACAACATAATCTTCTG+TGG | - | tig0028560:26159-26178 | Msa1438470:CDS | 35.0% | |
| ! | ATGGCCTTTGAATTTGAAGT+GGG | + | tig0028560:26246-26265 | None:intergenic | 35.0% |
| AAGGCCATGAAATCTAGTAA+TGG | - | tig0028560:26258-26277 | Msa1438470:CDS | 35.0% | |
| GGAACCATTACTAGATTTCA+TGG | + | tig0028560:26265-26284 | None:intergenic | 35.0% | |
| ! | ATAGCTAGCTTGAAGAGTAA+GGG | - | tig0028560:26285-26304 | Msa1438470:CDS | 35.0% |
| TTGAAGAGTAAGGGAAATGA+AGG | - | tig0028560:26294-26313 | Msa1438470:CDS | 35.0% | |
| GGAAATGAAGGAGAAGTAAA+GGG | - | tig0028560:26306-26325 | Msa1438470:CDS | 35.0% | |
| GAAGTCCAACAAAATCTAGA+TGG | - | tig0028560:26394-26413 | Msa1438470:CDS | 35.0% | |
| AGGTTGTTAAGAGTAGGAAT+AGG | - | tig0028560:26553-26572 | Msa1438470:CDS | 35.0% | |
| ATAGGAATAGTAAAGGGATG+TGG | - | tig0028560:26571-26590 | Msa1438470:CDS | 35.0% | |
| TTCTACTCAGATCAAGAAGC+AGG | - | tig0028560:25940-25959 | Msa1438470:CDS | 40.0% | |
| TCTACTCAGATCAAGAAGCA+GGG | - | tig0028560:25941-25960 | Msa1438470:CDS | 40.0% | |
| GGAATTTGGAAGGTCACAAA+GGG | + | tig0028560:26028-26047 | None:intergenic | 40.0% | |
| TGGAATTTGGAAGGTCACAA+AGG | + | tig0028560:26029-26048 | None:intergenic | 40.0% | |
| TTCCACAATTTCACCTCAAC+GGG | - | tig0028560:26044-26063 | Msa1438470:CDS | 40.0% | |
| ! | ATGGTGATCCTTGCTGTAAT+TGG | + | tig0028560:26079-26098 | None:intergenic | 40.0% |
| CATGGCCTTTGAATTTGAAG+TGG | + | tig0028560:26247-26266 | None:intergenic | 40.0% | |
| CTTACTCTTCAAGCTAGCTA+TGG | + | tig0028560:26286-26305 | None:intergenic | 40.0% | |
| ! | CATAGCTAGCTTGAAGAGTA+AGG | - | tig0028560:26284-26303 | Msa1438470:CDS | 40.0% |
| GGGAAATGAAGGAGAAGTAA+AGG | - | tig0028560:26305-26324 | Msa1438470:CDS | 40.0% | |
| ! | ACAACCTCTTTCCCATTTTC+CGG | + | tig0028560:26540-26559 | None:intergenic | 40.0% |
| GGAAAGAGGTTGTTAAGAGT+AGG | - | tig0028560:26547-26566 | Msa1438470:CDS | 40.0% | |
| TTCTACTCAGATCAAGAAGC+AGG | - | tig0028560:25940-25959 | Msa1438470:CDS | 40.0% | |
| TCTACTCAGATCAAGAAGCA+GGG | - | tig0028560:25941-25960 | Msa1438470:CDS | 40.0% | |
| GGAATTTGGAAGGTCACAAA+GGG | + | tig0028560:26028-26047 | None:intergenic | 40.0% | |
| TGGAATTTGGAAGGTCACAA+AGG | + | tig0028560:26029-26048 | None:intergenic | 40.0% | |
| TTCCACAATTTCACCTCAAC+GGG | - | tig0028560:26044-26063 | Msa1438470:CDS | 40.0% | |
| ! | ATGGTGATCCTTGCTGTAAT+TGG | + | tig0028560:26079-26098 | None:intergenic | 40.0% |
| CATGGCCTTTGAATTTGAAG+TGG | + | tig0028560:26247-26266 | None:intergenic | 40.0% | |
| CTTACTCTTCAAGCTAGCTA+TGG | + | tig0028560:26286-26305 | None:intergenic | 40.0% | |
| ! | CATAGCTAGCTTGAAGAGTA+AGG | - | tig0028560:26284-26303 | Msa1438470:CDS | 40.0% |
| GGGAAATGAAGGAGAAGTAA+AGG | - | tig0028560:26305-26324 | Msa1438470:CDS | 40.0% | |
| ! | ACAACCTCTTTCCCATTTTC+CGG | + | tig0028560:26540-26559 | None:intergenic | 40.0% |
| GGAAAGAGGTTGTTAAGAGT+AGG | - | tig0028560:26547-26566 | Msa1438470:CDS | 40.0% | |
| TCCACAATTTCACCTCAACG+GGG | - | tig0028560:26045-26064 | Msa1438470:CDS | 45.0% | |
| TTGGAAAAATCACCCCGTTG+AGG | + | tig0028560:26060-26079 | None:intergenic | 45.0% | |
| TGCTGGTGACTACACATCAA+TGG | - | tig0028560:26350-26369 | Msa1438470:CDS | 45.0% | |
| GCTGGTGACTACACATCAAT+GGG | - | tig0028560:26351-26370 | Msa1438470:CDS | 45.0% | |
| GTGACTACACATCAATGGGA+GGG | - | tig0028560:26355-26374 | Msa1438470:CDS | 45.0% | |
| GTCACTAAGTTGCATCTCCT+TGG | + | tig0028560:26463-26482 | None:intergenic | 45.0% | |
| GAAATAGACAGAGCAGAAGC+CGG | - | tig0028560:26487-26506 | Msa1438470:CDS | 45.0% | |
| ATGTTTCCGACACCGGAAAA+TGG | - | tig0028560:26525-26544 | Msa1438470:CDS | 45.0% | |
| TGTTTCCGACACCGGAAAAT+GGG | - | tig0028560:26526-26545 | Msa1438470:CDS | 45.0% | |
| ! | TCTTTCCCATTTTCCGGTGT+CGG | + | tig0028560:26534-26553 | None:intergenic | 45.0% |
| TCCACAATTTCACCTCAACG+GGG | - | tig0028560:26045-26064 | Msa1438470:CDS | 45.0% | |
| TTGGAAAAATCACCCCGTTG+AGG | + | tig0028560:26060-26079 | None:intergenic | 45.0% | |
| TGCTGGTGACTACACATCAA+TGG | - | tig0028560:26350-26369 | Msa1438470:CDS | 45.0% | |
| GCTGGTGACTACACATCAAT+GGG | - | tig0028560:26351-26370 | Msa1438470:CDS | 45.0% | |
| GTGACTACACATCAATGGGA+GGG | - | tig0028560:26355-26374 | Msa1438470:CDS | 45.0% | |
| GTCACTAAGTTGCATCTCCT+TGG | + | tig0028560:26463-26482 | None:intergenic | 45.0% | |
| GAAATAGACAGAGCAGAAGC+CGG | - | tig0028560:26487-26506 | Msa1438470:CDS | 45.0% | |
| ATGTTTCCGACACCGGAAAA+TGG | - | tig0028560:26525-26544 | Msa1438470:CDS | 45.0% | |
| TGTTTCCGACACCGGAAAAT+GGG | - | tig0028560:26526-26545 | Msa1438470:CDS | 45.0% | |
| ! | TCTTTCCCATTTTCCGGTGT+CGG | + | tig0028560:26534-26553 | None:intergenic | 45.0% |
| ACCCCGTTGAGGTGAAATTG+TGG | + | tig0028560:26049-26068 | None:intergenic | 50.0% | |
| GGTGACTACACATCAATGGG+AGG | - | tig0028560:26354-26373 | Msa1438470:CDS | 50.0% | |
| CTACACATCAATGGGAGGGA+AGG | - | tig0028560:26359-26378 | Msa1438470:CDS | 50.0% | |
| TTGGAATGTCGTCGAGTTCG+AGG | - | tig0028560:26430-26449 | Msa1438470:CDS | 50.0% | |
| GAGTTCGAGGATGTCTTCCA+AGG | - | tig0028560:26443-26462 | Msa1438470:CDS | 50.0% | |
| ! | GGTGTCGGAAACATCGTCAT+CGG | + | tig0028560:26519-26538 | None:intergenic | 50.0% |
| GATGACGATGTTTCCGACAC+CGG | - | tig0028560:26518-26537 | Msa1438470:CDS | 50.0% | |
| GACACCGGAAAATGGGAAAG+AGG | - | tig0028560:26533-26552 | Msa1438470:CDS | 50.0% | |
| ACCCCGTTGAGGTGAAATTG+TGG | + | tig0028560:26049-26068 | None:intergenic | 50.0% | |
| GGTGACTACACATCAATGGG+AGG | - | tig0028560:26354-26373 | Msa1438470:CDS | 50.0% | |
| CTACACATCAATGGGAGGGA+AGG | - | tig0028560:26359-26378 | Msa1438470:CDS | 50.0% | |
| TTGGAATGTCGTCGAGTTCG+AGG | - | tig0028560:26430-26449 | Msa1438470:CDS | 50.0% | |
| GAGTTCGAGGATGTCTTCCA+AGG | - | tig0028560:26443-26462 | Msa1438470:CDS | 50.0% | |
| ! | GGTGTCGGAAACATCGTCAT+CGG | + | tig0028560:26519-26538 | None:intergenic | 50.0% |
| GATGACGATGTTTCCGACAC+CGG | - | tig0028560:26518-26537 | Msa1438470:CDS | 50.0% | |
| GACACCGGAAAATGGGAAAG+AGG | - | tig0028560:26533-26552 | Msa1438470:CDS | 50.0% | |
| ATAGACAGAGCAGAAGCCGG+AGG | - | tig0028560:26490-26509 | Msa1438470:CDS | 55.0% | |
| TAGACAGAGCAGAAGCCGGA+GGG | - | tig0028560:26491-26510 | Msa1438470:CDS | 55.0% | |
| ATAGACAGAGCAGAAGCCGG+AGG | - | tig0028560:26490-26509 | Msa1438470:CDS | 55.0% | |
| TAGACAGAGCAGAAGCCGGA+GGG | - | tig0028560:26491-26510 | Msa1438470:CDS | 55.0% | |
| !! | ACATCGTCATCGGCTCCCTC+CGG | + | tig0028560:26509-26528 | None:intergenic | 60.0% |
| !! | ACATCGTCATCGGCTCCCTC+CGG | + | tig0028560:26509-26528 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0028560 | gene | 25925 | 26677 | 25925 | ID=Msa1438470;Name=Msa1438470 |
| tig0028560 | mRNA | 25925 | 26677 | 25925 | ID=Msa1438470-mRNA-1;Parent=Msa1438470;Name=Msa1438470-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|250 |
| tig0028560 | exon | 25925 | 26677 | 25925 | ID=Msa1438470-mRNA-1:exon:11262;Parent=Msa1438470-mRNA-1 |
| tig0028560 | CDS | 25925 | 26677 | 25925 | ID=Msa1438470-mRNA-1:cds;Parent=Msa1438470-mRNA-1 |
| Gene Sequence |
| Protein sequence |
