Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438470 | XP_003591967.1 | 95.238 | 252 | 10 | 2 | 1 | 250 | 1 | 252 | 6.03e-148 | 423 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0070120 | Msa1438470 | 0.830960 | 2.187405e-55 | -8.615850e-47 |
| Msa0073110 | Msa1438470 | 0.804171 | 2.454753e-49 | -8.615850e-47 |
| Msa0107830 | Msa1438470 | 0.824323 | 8.589592e-54 | -8.615850e-47 |
| Msa0117650 | Msa1438470 | 0.825474 | 4.597212e-54 | -8.615850e-47 |
| Msa0126430 | Msa1438470 | 0.806050 | 9.926679e-50 | -8.615850e-47 |
| Msa0129290 | Msa1438470 | 0.822102 | 2.834266e-53 | -8.615850e-47 |
| Msa0143640 | Msa1438470 | 0.801834 | 7.469305e-49 | -8.615850e-47 |
| Msa0150640 | Msa1438470 | 0.802506 | 5.432000e-49 | -8.615850e-47 |
| Msa0161230 | Msa1438470 | 0.805034 | 1.621746e-49 | -8.615850e-47 |
| Msa0185040 | Msa1438470 | 0.840655 | 7.627704e-58 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438470 | MtrunA17_Chr1g0200151 | 95.238 | 252 | 10 | 2 | 1 | 250 | 1 | 252 | 5.55e-152 | 423 |
Find 49 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATGTTGATGATCTTTGA+AGG | 0.488709 | tig0028560:+26387 | None:intergenic |
| AAGGCCATGAAATCTAGTAA+TGG | 0.615347 | tig0028560:-26322 | Msa1438470:CDS |
| ACATCGTCATCGGCTCCCTC+CGG | 0.586108 | tig0028560:+26074 | None:intergenic |
| ACATGTTGATGATCTTTGAA+GGG | 0.588773 | tig0028560:+26388 | None:intergenic |
| ACCCCGTTGAGGTGAAATTG+TGG | 0.594385 | tig0028560:+26534 | None:intergenic |
| ACTTGCTAATGATGTAGTAA+AGG | 0.515795 | tig0028560:-25939 | Msa1438470:CDS |
| AGGTTGTTAAGAGTAGGAAT+AGG | 0.451218 | tig0028560:-26027 | Msa1438470:CDS |
| AGTAGGAATAGGAATAGTAA+AGG | 0.434921 | tig0028560:-26016 | Msa1438470:CDS |
| ATAGACAGAGCAGAAGCCGG+AGG | 0.610980 | tig0028560:-26090 | Msa1438470:CDS |
| ATAGCTAGCTTGAAGAGTAA+GGG | 0.580739 | tig0028560:-26295 | Msa1438470:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTTATCATCATCATCATCA+TGG | + | tig0028560:26098-26117 | None:intergenic | 30.0% | |
| TAAACCCACTTCAAATTCAA+AGG | - | tig0028560:26239-26258 | Msa1438470:CDS | 30.0% | |
| AGTAGGAATAGGAATAGTAA+AGG | - | tig0028560:26564-26583 | Msa1438470:CDS | 30.0% | |
| GTAGGAATAGGAATAGTAAA+GGG | - | tig0028560:26565-26584 | Msa1438470:CDS | 30.0% | |
| AGTTATCATCATCATCATCA+TGG | + | tig0028560:26098-26117 | None:intergenic | 30.0% | |
| TAAACCCACTTCAAATTCAA+AGG | - | tig0028560:26239-26258 | Msa1438470:CDS | 30.0% | |
| AGTAGGAATAGGAATAGTAA+AGG | - | tig0028560:26564-26583 | Msa1438470:CDS | 30.0% | |
| GTAGGAATAGGAATAGTAAA+GGG | - | tig0028560:26565-26584 | Msa1438470:CDS | 30.0% | |
| GTGAAATTGTGGAATTTGGA+AGG | + | tig0028560:26038-26057 | None:intergenic | 35.0% | |
| TGAGGTGAAATTGTGGAATT+TGG | + | tig0028560:26042-26061 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0028560 | gene | 25925 | 26677 | 25925 | ID=Msa1438470;Name=Msa1438470 |
| tig0028560 | mRNA | 25925 | 26677 | 25925 | ID=Msa1438470-mRNA-1;Parent=Msa1438470;Name=Msa1438470-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|250 |
| tig0028560 | exon | 25925 | 26677 | 25925 | ID=Msa1438470-mRNA-1:exon:11262;Parent=Msa1438470-mRNA-1 |
| tig0028560 | CDS | 25925 | 26677 | 25925 | ID=Msa1438470-mRNA-1:cds;Parent=Msa1438470-mRNA-1 |
| Gene Sequence |
>Msa1438470 ATGGTGGCTAAAGAATTCTACTCAGATCAAGAAGCAGGGAATGAAGAAAGTACTTTGCTACAAGATGAGTATGATGATTATGAAGAAGAAGAAACTTTATCCCTTTGTGACCTTCCAAATTCCACAATTTCACCTCAACGGGGTGATTTTTCCAATTACAGCAAGGATCACCATGATGATGATGATGATAACTTGTTTGAGTTTTTCAGTGAAGAATTCAACACTTCAACAGTTCATGACAACATAATCTTCTGTGGAAAACTCATTCCCTTCAAAGATCATCAACATGTTCCTCATAACCAAAAAAATTGTGCTAAACCCACTTCAAATTCAAAGGCCATGAAATCTAGTAATGGTTCCATAGCTAGCTTGAAGAGTAAGGGAAATGAAGGAGAAGTAAAGGGTAGTTTGAATGTAAAGAGTTTTGCTGGTGACTACACATCAATGGGAGGGAAGGTTTCACTTGTGAGAAGTCCAACAAAATCTAGATGGTTTTTGTTTATGTTTGGAATGTCGTCGAGTTCGAGGATGTCTTCCAAGGAGATGCAACTTAGTGACATTAGAAATAGACAGAGCAGAAGCCGGAGGGAGCCGATGACGATGTTTCCGACACCGGAAAATGGGAAAGAGGTTGTTAAGAGTAGGAATAGGAATAGTAAAGGGATGTGGAAGATTTTGAAGTCAATTTCATTGGTTTTAGGATGCAGTAGTAGTAAACTTGCTAATGATGTAGTAAAGGCTGCTTTTGTGTGA |
| Protein sequence |
>Msa1438470 MVAKEFYSDQEAGNEESTLLQDEYDDYEEEETLSLCDLPNSTISPQRGDFSNYSKDHHDDDDDNLFEFFSEEFNTSTVHDNIIFCGKLIPFKDHQHVPHNQKNCAKPTSNSKAMKSSNGSIASLKSKGNEGEVKGSLNVKSFAGDYTSMGGKVSLVRSPTKSRWFLFMFGMSSSSRMSSKEMQLSDIRNRQSRSRREPMTMFPTPENGKEVVKSRNRNSKGMWKILKSISLVLGCSSSKLANDVVKAAFV* |
