AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1465480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465480 MtrunA17_Chr4g0027321 54.598 174 62 6 60 227 130 292 3.85e-49 162
Msa1465480 MtrunA17_Chr3g0119711 38.835 206 105 9 33 227 3 198 2.00e-30 111
Msa1465480 MtrunA17_Chr3g0120641 36.364 220 116 8 28 227 26 241 4.26e-21 88.2
Msa1465480 MtrunA17_Chr3g0120941 36.364 198 98 11 44 227 59 242 3.14e-18 80.5
Msa1465480 MtrunA17_Chr3g0120951 36.686 169 85 9 68 227 51 206 1.08e-17 78.6
Msa1465480 MtrunA17_Chr3g0120591 34.615 208 123 6 30 227 18 222 1.74e-17 78.2
Msa1465480 MtrunA17_Chr3g0120981 37.853 177 84 11 60 227 46 205 8.73e-16 73.2
Msa1465480 MtrunA17_Chr3g0120961 36.538 156 72 7 74 221 47 183 1.92e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTAATGTTTCTGTATAAT+TGG 0.200274 tig0045273:+1086 Msa1465480:three_prime_UTR
GCTTTAAAAGTAATGTAATA+TGG 0.262097 tig0045273:-817 None:intergenic
GATTTCAGCCGTTTCGATTT+CGG 0.272727 tig0045273:-193 None:intergenic
GTTGCCTTGGAAAGACTTCT+AGG 0.274118 tig0045273:+1003 Msa1465480:three_prime_UTR
TGGTTCTTTGTATACGAATA+AGG 0.284069 tig0045273:+435 Msa1465480:CDS
TGCCATGGATATGCTTGTTT+TGG 0.307797 tig0045273:+981 Msa1465480:three_prime_UTR
GAATCTTCTGGATCCGGTTT+CGG 0.312704 tig0045273:-334 None:intergenic
TCACGACGCTTATACCAAAA+TGG 0.333746 tig0045273:-412 None:intergenic
TCACTATACTCAGAATCTTC+TGG 0.339930 tig0045273:-346 None:intergenic
GAAATCGAAACGGCTGAAAT+CGG 0.340115 tig0045273:+195 Msa1465480:CDS
AATACACTCCGCTATGGTTA+TGG 0.350217 tig0045273:+950 Msa1465480:exon
ACTTTATCAAACACAAAAGT+TGG 0.407632 tig0045273:-748 None:intergenic
GTGTTTCAAAATGTCGAAAT+CGG 0.413339 tig0045273:+162 Msa1465480:exon
GCTGAAATCGGAACCGAAAC+CGG 0.414648 tig0045273:+321 Msa1465480:CDS
AAATCGAAACGGCTGAAATC+GGG 0.418006 tig0045273:+196 Msa1465480:CDS
ATGAAACACTCTTTGACAAC+AGG 0.423369 tig0045273:-916 None:intergenic
ACTTCTAGTCATTCATTCAA+TGG 0.426022 tig0045273:-1195 None:intergenic
CTACTTCTTTATATGCTCCT+AGG 0.435411 tig0045273:+794 Msa1465480:CDS
ATCTGTGGAGGAGGAGGAGG+AGG 0.436107 tig0045273:+291 Msa1465480:CDS
TACTCAGAATCTTCTGGATC+CGG 0.439841 tig0045273:-340 None:intergenic
TGCAGAGGGGTTTGAGAGTA+CGG 0.440533 tig0045273:-591 None:intergenic
AGTTAATTAAATAAATATGA+TGG 0.443216 tig0045273:+1029 Msa1465480:three_prime_UTR
GGAGGAGAAACGGCTGAAAT+CGG 0.450703 tig0045273:+309 Msa1465480:CDS
TTCATCTGTGGAGGAGGAGG+AGG 0.455235 tig0045273:+288 Msa1465480:CDS
CAAAATGTCGAAATCGGAAC+AGG 0.457893 tig0045273:+168 Msa1465480:exon
CTTCTAGTCATTCATTCAAT+GGG 0.464158 tig0045273:-1194 None:intergenic
CACTGTCCACCACGTTGTGC+TGG 0.467693 tig0045273:+532 Msa1465480:CDS
TACGCTGCAATACATGAGTC+TGG 0.467899 tig0045273:-882 None:intergenic
AAAGTAATGTAATATGGCCT+AGG 0.487724 tig0045273:-811 None:intergenic
CAATCAAACAAATATTAAGC+AGG 0.490074 tig0045273:+633 Msa1465480:CDS
CATTCAATGGGAGAAAATAC+AGG 0.498747 tig0045273:-1182 None:intergenic
TAAAGCAAAAGCAGAAGCAA+AGG 0.500294 tig0045273:+834 Msa1465480:CDS
TGAGGATGAGGAGGATAAGC+AGG 0.508361 tig0045273:+249 Msa1465480:CDS
AGAAAGAAATGAAACTCTAA+AGG 0.508555 tig0045273:-1154 None:intergenic
ACACGATGAAGATTCATATG+AGG 0.514467 tig0045273:+231 Msa1465480:CDS
CTTTGTATACGAATAAGGCT+TGG 0.515687 tig0045273:+440 Msa1465480:CDS
AGATTCATCTGTGGAGGAGG+AGG 0.522683 tig0045273:+285 Msa1465480:CDS
GTCTGGAAAGTTGTTAGAGC+AGG 0.523457 tig0045273:-865 None:intergenic
GTTAATTAAATAAATATGAT+GGG 0.528007 tig0045273:+1030 Msa1465480:three_prime_UTR
GTTGTCAAGTGTACCCATCC+TGG 0.532074 tig0045273:+769 Msa1465480:CDS
TGTATACGAATAAGGCTTGG+AGG 0.537395 tig0045273:+443 Msa1465480:CDS
AAAGCAAAAGCAGAAGCAAA+GGG 0.538089 tig0045273:+835 Msa1465480:CDS
ACAAGATTCATCTGTGGAGG+AGG 0.546974 tig0045273:+282 Msa1465480:CDS
AAATCTAATACACTCCGCTA+TGG 0.547572 tig0045273:+944 Msa1465480:exon
TGAAGATTCATATGAGGATG+AGG 0.548643 tig0045273:+237 Msa1465480:CDS
CACATCATCGCTTAAGTCGA+GGG 0.551344 tig0045273:-566 None:intergenic
TAAGGCTTGGAGGGATCGTA+AGG 0.553542 tig0045273:+453 Msa1465480:CDS
AACAGGAACCGAAATCGAAA+CGG 0.558710 tig0045273:+185 Msa1465480:CDS
CTTCAAAGCAGCATTGCAGA+GGG 0.559702 tig0045273:-605 None:intergenic
GCATATAAAGAAGTAGCACC+AGG 0.567371 tig0045273:-787 None:intergenic
TGTCCACCACGTTGTGCTGG+CGG 0.572730 tig0045273:+535 Msa1465480:CDS
CATTAGTGACATAAATAACA+TGG 0.577507 tig0045273:-1071 None:intergenic
GTATACGAATAAGGCTTGGA+GGG 0.581642 tig0045273:+444 Msa1465480:CDS
ATGAGACATGAATTTAGCAA+TGG 0.582250 tig0045273:-1244 None:intergenic
AACCAAAACAAGCATATCCA+TGG 0.587307 tig0045273:-983 None:intergenic
GTTATGGAATCTACCTGCCA+TGG 0.589249 tig0045273:+966 Msa1465480:three_prime_UTR
TAAAGAAGTAGCACCAGGAT+GGG 0.590468 tig0045273:-782 None:intergenic
AAAACAAGCATATCCATGGC+AGG 0.590966 tig0045273:-979 None:intergenic
GCACATCATCGCTTAAGTCG+AGG 0.599300 tig0045273:-567 None:intergenic
TACACCTAGAAGTCTTTCCA+AGG 0.607189 tig0045273:-1007 None:intergenic
ATAAAGAAGTAGCACCAGGA+TGG 0.610411 tig0045273:-783 None:intergenic
CAGCACAACGTGGTGGACAG+TGG 0.610706 tig0045273:-531 None:intergenic
GAAACAAGATTCATCTGTGG+AGG 0.613961 tig0045273:+279 Msa1465480:CDS
TCTTCAAAGCAGCATTGCAG+AGG 0.630589 tig0045273:-606 None:intergenic
AGATTCATATGAGGATGAGG+AGG 0.633765 tig0045273:+240 Msa1465480:CDS
TGAGTATAGTGAGGAAGACG+AGG 0.634076 tig0045273:+357 Msa1465480:CDS
GAAACGGCTGAAATCGGGAT+CGG 0.652067 tig0045273:+201 Msa1465480:CDS
AGAAGATTCTGAGTATAGTG+AGG 0.652619 tig0045273:+348 Msa1465480:CDS
GCAAAAGCAGAAGCAAAGGG+AGG 0.667232 tig0045273:+838 Msa1465480:CDS
CATGAAACAAGATTCATCTG+TGG 0.677002 tig0045273:+276 Msa1465480:CDS
ATACCGCCAGCACAACGTGG+TGG 0.689012 tig0045273:-538 None:intergenic
ACATCATCGCTTAAGTCGAG+GGG 0.690101 tig0045273:-565 None:intergenic
TTCAAAGCAGCATTGCAGAG+GGG 0.703047 tig0045273:-604 None:intergenic
GGTAGATTCCATAACCATAG+CGG 0.737684 tig0045273:-958 None:intergenic
ACAATACCGCCAGCACAACG+TGG 0.758364 tig0045273:-541 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTGTTTATTATTATTAATT+AGG + tig0045273:723-742 Msa1465480:intron 10.0%
!! AGTTAATTAAATAAATATGA+TGG + tig0045273:1029-1048 Msa1465480:three_prime_UTR 10.0%
!! GTTAATTAAATAAATATGAT+GGG + tig0045273:1030-1049 Msa1465480:three_prime_UTR 10.0%
!! CTTGTTTATTATTATTAATT+AGG + tig0045273:723-742 Msa1465480:intron 10.0%
!! AGTTAATTAAATAAATATGA+TGG + tig0045273:1029-1048 Msa1465480:three_prime_UTR 10.0%
!! GTTAATTAAATAAATATGAT+GGG + tig0045273:1030-1049 Msa1465480:three_prime_UTR 10.0%
!!! GCTTTAAAAGTAATGTAATA+TGG - tig0045273:820-839 None:intergenic 20.0%
!!! GCTTTAAAAGTAATGTAATA+TGG - tig0045273:820-839 None:intergenic 20.0%
! CAATCAAACAAATATTAAGC+AGG + tig0045273:633-652 Msa1465480:CDS 25.0%
! ACTTTATCAAACACAAAAGT+TGG - tig0045273:751-770 None:intergenic 25.0%
! CATTAGTGACATAAATAACA+TGG - tig0045273:1074-1093 None:intergenic 25.0%
!! CACTAATGTTTCTGTATAAT+TGG + tig0045273:1086-1105 Msa1465480:three_prime_UTR 25.0%
! AGAAAGAAATGAAACTCTAA+AGG - tig0045273:1157-1176 None:intergenic 25.0%
! CAATCAAACAAATATTAAGC+AGG + tig0045273:633-652 Msa1465480:CDS 25.0%
! ACTTTATCAAACACAAAAGT+TGG - tig0045273:751-770 None:intergenic 25.0%
! CATTAGTGACATAAATAACA+TGG - tig0045273:1074-1093 None:intergenic 25.0%
!! CACTAATGTTTCTGTATAAT+TGG + tig0045273:1086-1105 Msa1465480:three_prime_UTR 25.0%
! AGAAAGAAATGAAACTCTAA+AGG - tig0045273:1157-1176 None:intergenic 25.0%
!!! GATTTCGACATTTTGAAACA+CGG - tig0045273:163-182 None:intergenic 30.0%
GTGTTTCAAAATGTCGAAAT+CGG + tig0045273:162-181 Msa1465480:exon 30.0%
! ACAATGACAGTTTTCCATTT+TGG + tig0045273:398-417 Msa1465480:CDS 30.0%
TGGTTCTTTGTATACGAATA+AGG + tig0045273:435-454 Msa1465480:CDS 30.0%
CAAACAAATATTAAGCAGGT+TGG + tig0045273:637-656 Msa1465480:intron 30.0%
AAAGTAATGTAATATGGCCT+AGG - tig0045273:814-833 None:intergenic 30.0%
CTTCTAGTCATTCATTCAAT+GGG - tig0045273:1197-1216 None:intergenic 30.0%
ACTTCTAGTCATTCATTCAA+TGG - tig0045273:1198-1217 None:intergenic 30.0%
ATGAGACATGAATTTAGCAA+TGG - tig0045273:1247-1266 None:intergenic 30.0%
!!! GATTTCGACATTTTGAAACA+CGG - tig0045273:163-182 None:intergenic 30.0%
GTGTTTCAAAATGTCGAAAT+CGG + tig0045273:162-181 Msa1465480:exon 30.0%
! ACAATGACAGTTTTCCATTT+TGG + tig0045273:398-417 Msa1465480:CDS 30.0%
TGGTTCTTTGTATACGAATA+AGG + tig0045273:435-454 Msa1465480:CDS 30.0%
CAAACAAATATTAAGCAGGT+TGG + tig0045273:637-656 Msa1465480:intron 30.0%
AAAGTAATGTAATATGGCCT+AGG - tig0045273:814-833 None:intergenic 30.0%
CTTCTAGTCATTCATTCAAT+GGG - tig0045273:1197-1216 None:intergenic 30.0%
ACTTCTAGTCATTCATTCAA+TGG - tig0045273:1198-1217 None:intergenic 30.0%
ATGAGACATGAATTTAGCAA+TGG - tig0045273:1247-1266 None:intergenic 30.0%
ACACGATGAAGATTCATATG+AGG + tig0045273:231-250 Msa1465480:CDS 35.0%
TGAAGATTCATATGAGGATG+AGG + tig0045273:237-256 Msa1465480:CDS 35.0%
CATGAAACAAGATTCATCTG+TGG + tig0045273:276-295 Msa1465480:CDS 35.0%
TCACTATACTCAGAATCTTC+TGG - tig0045273:349-368 None:intergenic 35.0%
AGAAGATTCTGAGTATAGTG+AGG + tig0045273:348-367 Msa1465480:CDS 35.0%
CTTTGTATACGAATAAGGCT+TGG + tig0045273:440-459 Msa1465480:CDS 35.0%
CTACTTCTTTATATGCTCCT+AGG + tig0045273:794-813 Msa1465480:CDS 35.0%
TAAAGCAAAAGCAGAAGCAA+AGG + tig0045273:834-853 Msa1465480:CDS 35.0%
AAAGCAAAAGCAGAAGCAAA+GGG + tig0045273:835-854 Msa1465480:CDS 35.0%
ATGAAACACTCTTTGACAAC+AGG - tig0045273:919-938 None:intergenic 35.0%
AAATCTAATACACTCCGCTA+TGG + tig0045273:944-963 Msa1465480:exon 35.0%
AACCAAAACAAGCATATCCA+TGG - tig0045273:986-1005 None:intergenic 35.0%
CATTCAATGGGAGAAAATAC+AGG - tig0045273:1185-1204 None:intergenic 35.0%
ACACGATGAAGATTCATATG+AGG + tig0045273:231-250 Msa1465480:CDS 35.0%
TGAAGATTCATATGAGGATG+AGG + tig0045273:237-256 Msa1465480:CDS 35.0%
CATGAAACAAGATTCATCTG+TGG + tig0045273:276-295 Msa1465480:CDS 35.0%
TCACTATACTCAGAATCTTC+TGG - tig0045273:349-368 None:intergenic 35.0%
AGAAGATTCTGAGTATAGTG+AGG + tig0045273:348-367 Msa1465480:CDS 35.0%
CTTTGTATACGAATAAGGCT+TGG + tig0045273:440-459 Msa1465480:CDS 35.0%
CTACTTCTTTATATGCTCCT+AGG + tig0045273:794-813 Msa1465480:CDS 35.0%
TAAAGCAAAAGCAGAAGCAA+AGG + tig0045273:834-853 Msa1465480:CDS 35.0%
AAAGCAAAAGCAGAAGCAAA+GGG + tig0045273:835-854 Msa1465480:CDS 35.0%
ATGAAACACTCTTTGACAAC+AGG - tig0045273:919-938 None:intergenic 35.0%
AAATCTAATACACTCCGCTA+TGG + tig0045273:944-963 Msa1465480:exon 35.0%
AACCAAAACAAGCATATCCA+TGG - tig0045273:986-1005 None:intergenic 35.0%
CATTCAATGGGAGAAAATAC+AGG - tig0045273:1185-1204 None:intergenic 35.0%
!!! TCGACATTTTGAAACACGGA+AGG - tig0045273:159-178 None:intergenic 40.0%
CAAAATGTCGAAATCGGAAC+AGG + tig0045273:168-187 Msa1465480:exon 40.0%
AACAGGAACCGAAATCGAAA+CGG + tig0045273:185-204 Msa1465480:CDS 40.0%
GATTTCAGCCGTTTCGATTT+CGG - tig0045273:196-215 None:intergenic 40.0%
GAAATCGAAACGGCTGAAAT+CGG + tig0045273:195-214 Msa1465480:CDS 40.0%
AAATCGAAACGGCTGAAATC+GGG + tig0045273:196-215 Msa1465480:CDS 40.0%
AGATTCATATGAGGATGAGG+AGG + tig0045273:240-259 Msa1465480:CDS 40.0%
GAAACAAGATTCATCTGTGG+AGG + tig0045273:279-298 Msa1465480:CDS 40.0%
TACTCAGAATCTTCTGGATC+CGG - tig0045273:343-362 None:intergenic 40.0%
TCACGACGCTTATACCAAAA+TGG - tig0045273:415-434 None:intergenic 40.0%
!! TTTTGGTATAAGCGTCGTGA+TGG + tig0045273:415-434 Msa1465480:CDS 40.0%
TGTATACGAATAAGGCTTGG+AGG + tig0045273:443-462 Msa1465480:CDS 40.0%
GTATACGAATAAGGCTTGGA+GGG + tig0045273:444-463 Msa1465480:CDS 40.0%
TAAAGAAGTAGCACCAGGAT+GGG - tig0045273:785-804 None:intergenic 40.0%
ATAAAGAAGTAGCACCAGGA+TGG - tig0045273:786-805 None:intergenic 40.0%
GCATATAAAGAAGTAGCACC+AGG - tig0045273:790-809 None:intergenic 40.0%
AATACACTCCGCTATGGTTA+TGG + tig0045273:950-969 Msa1465480:exon 40.0%
GGTAGATTCCATAACCATAG+CGG - tig0045273:961-980 None:intergenic 40.0%
AAAACAAGCATATCCATGGC+AGG - tig0045273:982-1001 None:intergenic 40.0%
! TGCCATGGATATGCTTGTTT+TGG + tig0045273:981-1000 Msa1465480:three_prime_UTR 40.0%
!!! TATGCTTGTTTTGGTTGCCT+TGG + tig0045273:990-1009 Msa1465480:three_prime_UTR 40.0%
TACACCTAGAAGTCTTTCCA+AGG - tig0045273:1010-1029 None:intergenic 40.0%
!!! TCGACATTTTGAAACACGGA+AGG - tig0045273:159-178 None:intergenic 40.0%
CAAAATGTCGAAATCGGAAC+AGG + tig0045273:168-187 Msa1465480:exon 40.0%
AACAGGAACCGAAATCGAAA+CGG + tig0045273:185-204 Msa1465480:CDS 40.0%
GATTTCAGCCGTTTCGATTT+CGG - tig0045273:196-215 None:intergenic 40.0%
GAAATCGAAACGGCTGAAAT+CGG + tig0045273:195-214 Msa1465480:CDS 40.0%
AAATCGAAACGGCTGAAATC+GGG + tig0045273:196-215 Msa1465480:CDS 40.0%
AGATTCATATGAGGATGAGG+AGG + tig0045273:240-259 Msa1465480:CDS 40.0%
GAAACAAGATTCATCTGTGG+AGG + tig0045273:279-298 Msa1465480:CDS 40.0%
TACTCAGAATCTTCTGGATC+CGG - tig0045273:343-362 None:intergenic 40.0%
TCACGACGCTTATACCAAAA+TGG - tig0045273:415-434 None:intergenic 40.0%
!! TTTTGGTATAAGCGTCGTGA+TGG + tig0045273:415-434 Msa1465480:CDS 40.0%
TGTATACGAATAAGGCTTGG+AGG + tig0045273:443-462 Msa1465480:CDS 40.0%
GTATACGAATAAGGCTTGGA+GGG + tig0045273:444-463 Msa1465480:CDS 40.0%
TAAAGAAGTAGCACCAGGAT+GGG - tig0045273:785-804 None:intergenic 40.0%
ATAAAGAAGTAGCACCAGGA+TGG - tig0045273:786-805 None:intergenic 40.0%
GCATATAAAGAAGTAGCACC+AGG - tig0045273:790-809 None:intergenic 40.0%
AATACACTCCGCTATGGTTA+TGG + tig0045273:950-969 Msa1465480:exon 40.0%
GGTAGATTCCATAACCATAG+CGG - tig0045273:961-980 None:intergenic 40.0%
AAAACAAGCATATCCATGGC+AGG - tig0045273:982-1001 None:intergenic 40.0%
! TGCCATGGATATGCTTGTTT+TGG + tig0045273:981-1000 Msa1465480:three_prime_UTR 40.0%
!!! TATGCTTGTTTTGGTTGCCT+TGG + tig0045273:990-1009 Msa1465480:three_prime_UTR 40.0%
TACACCTAGAAGTCTTTCCA+AGG - tig0045273:1010-1029 None:intergenic 40.0%
ACAAGATTCATCTGTGGAGG+AGG + tig0045273:282-301 Msa1465480:CDS 45.0%
GAATCTTCTGGATCCGGTTT+CGG - tig0045273:337-356 None:intergenic 45.0%
TGAGTATAGTGAGGAAGACG+AGG + tig0045273:357-376 Msa1465480:CDS 45.0%
ACATCATCGCTTAAGTCGAG+GGG - tig0045273:568-587 None:intergenic 45.0%
CACATCATCGCTTAAGTCGA+GGG - tig0045273:569-588 None:intergenic 45.0%
TTCAAAGCAGCATTGCAGAG+GGG - tig0045273:607-626 None:intergenic 45.0%
CTTCAAAGCAGCATTGCAGA+GGG - tig0045273:608-627 None:intergenic 45.0%
TCTTCAAAGCAGCATTGCAG+AGG - tig0045273:609-628 None:intergenic 45.0%
GTCTGGAAAGTTGTTAGAGC+AGG - tig0045273:868-887 None:intergenic 45.0%
TACGCTGCAATACATGAGTC+TGG - tig0045273:885-904 None:intergenic 45.0%
GTTATGGAATCTACCTGCCA+TGG + tig0045273:966-985 Msa1465480:three_prime_UTR 45.0%
GTTGCCTTGGAAAGACTTCT+AGG + tig0045273:1003-1022 Msa1465480:three_prime_UTR 45.0%
ACAAGATTCATCTGTGGAGG+AGG + tig0045273:282-301 Msa1465480:CDS 45.0%
GAATCTTCTGGATCCGGTTT+CGG - tig0045273:337-356 None:intergenic 45.0%
TGAGTATAGTGAGGAAGACG+AGG + tig0045273:357-376 Msa1465480:CDS 45.0%
ACATCATCGCTTAAGTCGAG+GGG - tig0045273:568-587 None:intergenic 45.0%
CACATCATCGCTTAAGTCGA+GGG - tig0045273:569-588 None:intergenic 45.0%
TTCAAAGCAGCATTGCAGAG+GGG - tig0045273:607-626 None:intergenic 45.0%
CTTCAAAGCAGCATTGCAGA+GGG - tig0045273:608-627 None:intergenic 45.0%
TCTTCAAAGCAGCATTGCAG+AGG - tig0045273:609-628 None:intergenic 45.0%
GTCTGGAAAGTTGTTAGAGC+AGG - tig0045273:868-887 None:intergenic 45.0%
TACGCTGCAATACATGAGTC+TGG - tig0045273:885-904 None:intergenic 45.0%
GTTATGGAATCTACCTGCCA+TGG + tig0045273:966-985 Msa1465480:three_prime_UTR 45.0%
GTTGCCTTGGAAAGACTTCT+AGG + tig0045273:1003-1022 Msa1465480:three_prime_UTR 45.0%
!! TTGTTTATTATTATTAATTA+GGG + tig0045273:724-743 Msa1465480:intron 5.0%
!! TTGTTTATTATTATTAATTA+GGG + tig0045273:724-743 Msa1465480:intron 5.0%
GAAACGGCTGAAATCGGGAT+CGG + tig0045273:201-220 Msa1465480:CDS 50.0%
TGAGGATGAGGAGGATAAGC+AGG + tig0045273:249-268 Msa1465480:CDS 50.0%
AGATTCATCTGTGGAGGAGG+AGG + tig0045273:285-304 Msa1465480:CDS 50.0%
GGAGGAGAAACGGCTGAAAT+CGG + tig0045273:309-328 Msa1465480:CDS 50.0%
GCTGAAATCGGAACCGAAAC+CGG + tig0045273:321-340 Msa1465480:CDS 50.0%
TAAGGCTTGGAGGGATCGTA+AGG + tig0045273:453-472 Msa1465480:CDS 50.0%
GCACATCATCGCTTAAGTCG+AGG - tig0045273:570-589 None:intergenic 50.0%
TGCAGAGGGGTTTGAGAGTA+CGG - tig0045273:594-613 None:intergenic 50.0%
GTTGTCAAGTGTACCCATCC+TGG + tig0045273:769-788 Msa1465480:CDS 50.0%
GCAAAAGCAGAAGCAAAGGG+AGG + tig0045273:838-857 Msa1465480:CDS 50.0%
GAAACGGCTGAAATCGGGAT+CGG + tig0045273:201-220 Msa1465480:CDS 50.0%
TGAGGATGAGGAGGATAAGC+AGG + tig0045273:249-268 Msa1465480:CDS 50.0%
AGATTCATCTGTGGAGGAGG+AGG + tig0045273:285-304 Msa1465480:CDS 50.0%
GGAGGAGAAACGGCTGAAAT+CGG + tig0045273:309-328 Msa1465480:CDS 50.0%
GCTGAAATCGGAACCGAAAC+CGG + tig0045273:321-340 Msa1465480:CDS 50.0%
TAAGGCTTGGAGGGATCGTA+AGG + tig0045273:453-472 Msa1465480:CDS 50.0%
GCACATCATCGCTTAAGTCG+AGG - tig0045273:570-589 None:intergenic 50.0%
TGCAGAGGGGTTTGAGAGTA+CGG - tig0045273:594-613 None:intergenic 50.0%
GTTGTCAAGTGTACCCATCC+TGG + tig0045273:769-788 Msa1465480:CDS 50.0%
GCAAAAGCAGAAGCAAAGGG+AGG + tig0045273:838-857 Msa1465480:CDS 50.0%
TTCATCTGTGGAGGAGGAGG+AGG + tig0045273:288-307 Msa1465480:CDS 55.0%
AGGAGGAGGAGGAGGAGAAA+CGG + tig0045273:299-318 Msa1465480:CDS 55.0%
ACAATACCGCCAGCACAACG+TGG - tig0045273:544-563 None:intergenic 55.0%
TTCATCTGTGGAGGAGGAGG+AGG + tig0045273:288-307 Msa1465480:CDS 55.0%
AGGAGGAGGAGGAGGAGAAA+CGG + tig0045273:299-318 Msa1465480:CDS 55.0%
ACAATACCGCCAGCACAACG+TGG - tig0045273:544-563 None:intergenic 55.0%
ATCTGTGGAGGAGGAGGAGG+AGG + tig0045273:291-310 Msa1465480:CDS 60.0%
!! CAGCACAACGTGGTGGACAG+TGG - tig0045273:534-553 None:intergenic 60.0%
CACTGTCCACCACGTTGTGC+TGG + tig0045273:532-551 Msa1465480:CDS 60.0%
TGTCCACCACGTTGTGCTGG+CGG + tig0045273:535-554 Msa1465480:CDS 60.0%
ATACCGCCAGCACAACGTGG+TGG - tig0045273:541-560 None:intergenic 60.0%
ATCTGTGGAGGAGGAGGAGG+AGG + tig0045273:291-310 Msa1465480:CDS 60.0%
!! CAGCACAACGTGGTGGACAG+TGG - tig0045273:534-553 None:intergenic 60.0%
CACTGTCCACCACGTTGTGC+TGG + tig0045273:532-551 Msa1465480:CDS 60.0%
TGTCCACCACGTTGTGCTGG+CGG + tig0045273:535-554 Msa1465480:CDS 60.0%
ATACCGCCAGCACAACGTGG+TGG - tig0045273:541-560 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0045273 gene 142 1266 142 ID=Msa1465480;Name=Msa1465480
tig0045273 mRNA 142 1266 142 ID=Msa1465480-mRNA-1;Parent=Msa1465480;Name=Msa1465480-mRNA-1;_AED=0.04;_eAED=0.04;_QI=30|1|1|1|1|1|2|315|229
tig0045273 exon 142 654 142 ID=Msa1465480-mRNA-1:exon:24803;Parent=Msa1465480-mRNA-1
tig0045273 exon 745 1266 745 ID=Msa1465480-mRNA-1:exon:24804;Parent=Msa1465480-mRNA-1
tig0045273 five_prime_UTR 142 171 142 ID=Msa1465480-mRNA-1:five_prime_utr;Parent=Msa1465480-mRNA-1
tig0045273 CDS 172 654 172 ID=Msa1465480-mRNA-1:cds;Parent=Msa1465480-mRNA-1
tig0045273 CDS 745 951 745 ID=Msa1465480-mRNA-1:cds;Parent=Msa1465480-mRNA-1
tig0045273 three_prime_UTR 952 1266 952 ID=Msa1465480-mRNA-1:three_prime_utr;Parent=Msa1465480-mRNA-1
Gene Sequence

>Msa1465480

ATGTCGAAATCGGAACAGGAACCGAAATCGAAACGGCTGAAATCGGGATCGGAACAGAAACACGATGAAGATTCATATGAGGATGAGGAGGATAAGCAGGAATTCATGAAACAAGATTCATCTGTGGAGGAGGAGGAGGAGGAGAAACGGCTGAAATCGGAACCGAAACCGGATCCAGAAGATTCTGAGTATAGTGAGGAAGACGAGGAGAACTTGTCTGTGCATGACAATGACAGTTTTCCATTTTGGTATAAGCGTCGTGATGGTTCTTTGTATACGAATAAGGCTTGGAGGGATCGTAAGGCTAAAATTCTTGACTATAAAAATCGCTCTAGCAAACTAAGTGTCTACGATGCTATCCACTGTCCACCACGTTGTGCTGGCGGTATTGTACCCCTCGACTTAAGCGATGATGTGCTCCGTACTCTCAAACCCCTCTGCAATGCTGCTTTGAAGATTTACAATCAAACAAATATTAAGCAGGGTCCAACTTTTGTGTTTGATAAAGTTGTCAAGTGTACCCATCCTGGTGCTACTTCTTTATATGCTCCTAGGCCATATTACATTACTTTTAAAGCAAAAGCAGAAGCAAAGGGAGGAAGACCTGCTCTAACAACTTTCCAGACTCATGTATTGCAGCGTAAAGATAAAGATCCTGTTGTCAAAGAGTGTTTCATTAAGAAAATCTAA
Protein sequence

>Msa1465480

MSKSEQEPKSKRLKSGSEQKHDEDSYEDEEDKQEFMKQDSSVEEEEEEKRLKSEPKPDPEDSEYSEEDEENLSVHDNDSFPFWYKRRDGSLYTNKAWRDRKAKILDYKNRSSKLSVYDAIHCPPRCAGGIVPLDLSDDVLRTLKPLCNAALKIYNQTNIKQGPTFVFDKVVKCTHPGATSLYAPRPYYITFKAKAEAKGGRPALTTFQTHVLQRKDKDPVVKECFIKKI*