AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002546

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002546.t1 MTR_2g015500 82.716 243 32 1 1 233 1 243 3.38e-151 420
MS.gene002546.t1 MTR_2g015510 80.833 240 38 1 2 233 3 242 1.06e-146 409
MS.gene002546.t1 MTR_2g015480 70.435 230 39 6 12 229 65 277 1.20e-100 295
MS.gene002546.t1 MTR_4g105680 45.581 215 110 2 25 232 51 265 5.27e-59 187
MS.gene002546.t1 MTR_7g068530 37.864 206 120 2 4 205 6 207 9.11e-49 160
MS.gene002546.t1 MTR_4g016510 37.379 206 123 3 4 205 6 209 5.76e-48 159
MS.gene002546.t1 MTR_7g068550 37.864 206 120 3 4 205 6 207 1.66e-47 157
MS.gene002546.t1 MTR_7g068460 36.538 208 124 3 2 205 31 234 2.29e-44 150
MS.gene002546.t1 MTR_3g070460 35.577 208 124 3 4 205 6 209 1.17e-42 145
MS.gene002546.t1 MTR_7g068500 36.364 209 123 4 2 205 30 233 2.93e-42 145
MS.gene002546.t1 MTR_7g068470 34.466 206 127 4 4 205 2 203 3.39e-40 138
MS.gene002546.t1 MTR_7g068470 34.951 206 122 5 4 205 2 199 9.51e-40 137
MS.gene002546.t1 MTR_7g068450 31.646 237 148 3 4 229 2 235 4.67e-37 130
MS.gene002546.t1 MTR_4g016500 32.353 136 64 3 33 168 277 384 1.25e-17 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002546.t1 AT5G45920 66.949 236 71 1 4 232 3 238 3.64e-116 332
MS.gene002546.t1 AT5G45920 66.949 236 71 1 4 232 3 238 3.64e-116 332
MS.gene002546.t1 AT5G45920 69.613 181 48 1 4 177 3 183 1.44e-90 265
MS.gene002546.t1 AT5G45920 69.613 181 48 1 4 177 3 183 1.44e-90 265
MS.gene002546.t1 AT5G62930 52.542 236 105 2 4 232 2 237 3.50e-80 240
MS.gene002546.t1 AT5G62930 52.542 236 105 2 4 232 28 263 1.47e-79 240
MS.gene002546.t1 AT5G62930 49.438 178 87 1 58 232 2 179 1.62e-53 171
MS.gene002546.t1 AT2G38180 38.049 205 119 3 4 204 6 206 6.92e-44 150
MS.gene002546.t1 AT3G11210 34.615 208 126 3 4 205 6 209 1.36e-42 145

Find 61 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTCATTTGTCCTTTC+AGG 0.154934 2.1:-69972270 None:intergenic
TTGGATCAATATCAGCTAAA+AGG 0.242460 2.1:-69972765 None:intergenic
AAATTCCTTTCATTGATCTT+TGG 0.243390 2.1:+69972337 MS.gene002546:CDS
AAGTCGTGCTTCTTCATCTA+TGG 0.253796 2.1:-69971889 None:intergenic
AATGCCTTCAGTGGATCATT+TGG 0.343903 2.1:-69972784 None:intergenic
TCCTTTGGTGTTGGAGGATG+GGG 0.361517 2.1:+69969540 MS.gene002546:CDS
CAAAGCAACAGGAGCAGTTT+CGG 0.379507 2.1:-69971064 None:intergenic
GAAAGGAATTTGACACTCAT+TGG 0.387705 2.1:-69972325 None:intergenic
CTATGGGAGGAGGAGTTATA+AGG 0.399420 2.1:-69971872 None:intergenic
TGGATCAATATCAGCTAAAA+GGG 0.400368 2.1:-69972764 None:intergenic
GACGGTTTGCATCTCACTAA+TGG 0.400983 2.1:+69972658 MS.gene002546:CDS
TCGATCACTGAAGAATCCTT+TGG 0.438191 2.1:+69969525 MS.gene002546:CDS
AGGGAATCAATTTGTGTTTG+AGG 0.442405 2.1:+69972681 MS.gene002546:CDS
ACTAAAATGCAACGGTTCCC+TGG 0.444415 2.1:+69972360 MS.gene002546:CDS
AAGAAAACTGTCAAAGCAAC+AGG 0.453323 2.1:-69971075 None:intergenic
AATCCTTTGGTGTTGGAGGA+TGG 0.456779 2.1:+69969538 MS.gene002546:CDS
ATCCTTTGGTGTTGGAGGAT+GGG 0.456978 2.1:+69969539 MS.gene002546:CDS
GTCCTTTCAGGAAGCCCCTC+CGG 0.465657 2.1:-69972258 None:intergenic
AAATGCAACGGTTCCCTGGA+TGG 0.484613 2.1:+69972364 MS.gene002546:CDS
GGCACTGAAGGTGTTGGAAA+GGG 0.491788 2.1:+69971008 MS.gene002546:CDS
TATAACAAAACATGACTAAG+AGG 0.493140 2.1:+69969478 None:intergenic
ACTGAAGAATCCTTTGGTGT+TGG 0.507462 2.1:+69969531 MS.gene002546:CDS
TAGATGAAGAAGCACGACTT+AGG 0.509915 2.1:+69971892 MS.gene002546:CDS
GAAGAATCCTTTGGTGTTGG+AGG 0.526860 2.1:+69969534 MS.gene002546:CDS
TGCCAACTATTTCTCTCGTA+CGG 0.529977 2.1:+69969572 MS.gene002546:CDS
ATCCGGAGGGGCTTCCTGAA+AGG 0.532035 2.1:+69972256 MS.gene002546:CDS
TTATTTGTTTGTGAACAATC+CGG 0.532846 2.1:+69972239 MS.gene002546:CDS
TCACCGAAAAGATATATTTG+TGG 0.535472 2.1:-69969501 None:intergenic
AGTCGTGCTTCTTCATCTAT+GGG 0.542373 2.1:-69971888 None:intergenic
AATGCAACGGTTCCCTGGAT+GGG 0.542822 2.1:+69972365 MS.gene002546:CDS
GAAAGGACAAATGAAGCTGC+TGG 0.551991 2.1:+69972273 MS.gene002546:CDS
ATCTTTGGACTAAAATGCAA+CGG 0.555620 2.1:+69972352 MS.gene002546:CDS
TACCGTACGAGAGAAATAGT+TGG 0.562274 2.1:-69969574 None:intergenic
GGGCACTGAAGGTGTTGGAA+AGG 0.563729 2.1:+69971007 MS.gene002546:CDS
ATAAGGACAACATTAGTTGA+CGG 0.572638 2.1:-69971855 None:intergenic
GCTTCTTCATCTATGGGAGG+AGG 0.576690 2.1:-69971882 None:intergenic
TGTGAGTCTAGAATCTATGC+AGG 0.579365 2.1:+69972732 MS.gene002546:CDS
TGTTCCTCTTCATGAGTACA+AGG 0.584094 2.1:+69971146 MS.gene002546:CDS
TTAGTCCAAAGATCAATGAA+AGG 0.592788 2.1:-69972342 None:intergenic
AAAAGGAGACAATGGAGCGA+AGG 0.597580 2.1:-69971173 None:intergenic
ACCCCATCCTCCAACACCAA+AGG 0.599552 2.1:-69969541 None:intergenic
TGGTGTTCAGAGGGTATCGT+GGG 0.600203 2.1:+69970971 MS.gene002546:CDS
GGTTTGCATCTCACTAATGG+AGG 0.604059 2.1:+69972661 MS.gene002546:CDS
TTGTCCTTGTACTCATGAAG+AGG 0.610871 2.1:-69971150 None:intergenic
TGATCCAAATGATCCACTGA+AGG 0.615047 2.1:+69972780 MS.gene002546:CDS
TAACACACGATGGGCACTGA+AGG 0.617976 2.1:+69970996 MS.gene002546:CDS
AGGCTGATGTGGTGTTCAGA+GGG 0.618365 2.1:+69970962 MS.gene002546:intron
ACGATGGGCACTGAAGGTGT+TGG 0.625863 2.1:+69971002 MS.gene002546:CDS
GTGGTGTTCAGAGGGTATCG+TGG 0.629790 2.1:+69970970 MS.gene002546:CDS
GGATCAATATCAGCTAAAAG+GGG 0.632239 2.1:-69972763 None:intergenic
TGCTAAAGCATGCATTTCTG+TGG 0.632567 2.1:+69972302 MS.gene002546:CDS
TAGGCTGATGTGGTGTTCAG+AGG 0.635589 2.1:+69970961 MS.gene002546:intron
TTGTTTGTGAACAATCCGGA+GGG 0.641948 2.1:+69972243 MS.gene002546:CDS
TCGTGGGTATAACACACGAT+GGG 0.646142 2.1:+69970987 MS.gene002546:CDS
TGTTTGTGAACAATCCGGAG+GGG 0.648290 2.1:+69972244 MS.gene002546:CDS
CGTGCTTCTTCATCTATGGG+AGG 0.659087 2.1:-69971885 None:intergenic
TTTGTTTGTGAACAATCCGG+AGG 0.659446 2.1:+69972242 MS.gene002546:CDS
GTTTGCATCTCACTAATGGA+GGG 0.661505 2.1:+69972662 MS.gene002546:CDS
GATCAATATCAGCTAAAAGG+GGG 0.672744 2.1:-69972762 None:intergenic
ATCGTGGGTATAACACACGA+TGG 0.679127 2.1:+69970986 MS.gene002546:CDS
GCTCGTGTGATAGGCTGATG+TGG 0.704764 2.1:+69970951 MS.gene002546:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAAAAAAATTATTTT+TGG - chr2.1:69970800-69970819 None:intergenic 0.0%
!! GTATAAATAAATCATATAAT+TGG + chr2.1:69971796-69971815 MS.gene002546:intron 10.0%
!!! CATTAGTATTTTAAATTAAA+TGG - chr2.1:69972477-69972496 None:intergenic 10.0%
!!! CTTTAAAATGATTTTTTTTT+TGG + chr2.1:69970220-69970239 MS.gene002546:intron 10.0%
!!! TTATTAAAATTAAAAGTGTT+TGG + chr2.1:69970052-69970071 MS.gene002546:intron 10.0%
!! AAAATTCTAAACATTTAAAG+TGG - chr2.1:69971730-69971749 None:intergenic 15.0%
!! ATAAATATAAATGACGTAAA+AGG + chr2.1:69970099-69970118 MS.gene002546:intron 15.0%
!! ATATGATTTATTTATACAAG+AGG - chr2.1:69971793-69971812 None:intergenic 15.0%
!! ATTAAATATGCAAAGATAAT+TGG + chr2.1:69972522-69972541 MS.gene002546:intron 15.0%
!! TATTATATGAATTATGAACA+AGG + chr2.1:69970371-69970390 MS.gene002546:intron 15.0%
!!! AAAAATCTTATTTTAAAAGC+CGG - chr2.1:69971301-69971320 None:intergenic 15.0%
!!! AATAGATAAAAGGTTAATTT+TGG + chr2.1:69970526-69970545 MS.gene002546:intron 15.0%
!!! AATGTTTTTATTTTTCGTTA+TGG + chr2.1:69969722-69969741 MS.gene002546:intron 15.0%
!!! ACATTTGATTTATCATATAA+TGG - chr2.1:69971568-69971587 None:intergenic 15.0%
!!! CTTAATTTTGTTTTTTCTTT+AGG + chr2.1:69971645-69971664 MS.gene002546:intron 15.0%
!! AAATCAAATGTAGAAATAGT+AGG + chr2.1:69971507-69971526 MS.gene002546:intron 20.0%
!! AAATCAAATGTAGAAATTGT+AGG + chr2.1:69971577-69971596 MS.gene002546:intron 20.0%
!! AACTTATTAATTAGTGAGTT+GGG + chr2.1:69971444-69971463 MS.gene002546:intron 20.0%
!! AAGAAAACCTAATAAAAACA+AGG - chr2.1:69972145-69972164 None:intergenic 20.0%
!! AGAAAACCTAATAAAAACAA+GGG - chr2.1:69972144-69972163 None:intergenic 20.0%
!! ATAAAAAAACTAAGAGATGA+AGG + chr2.1:69972709-69972728 MS.gene002546:CDS 20.0%
!! TAAAAACAATGACAAAATCA+AGG - chr2.1:69971939-69971958 None:intergenic 20.0%
!! TAAAATACTAATGAACATGA+TGG + chr2.1:69972484-69972503 MS.gene002546:intron 20.0%
!! TAAACATAAATGAAGAGTTA+AGG + chr2.1:69971272-69971291 MS.gene002546:intron 20.0%
!! TAATAAATAAACCCGTTATT+AGG - chr2.1:69971351-69971370 None:intergenic 20.0%
!! TATAGAAGTTAATCATACTT+AGG + chr2.1:69972194-69972213 MS.gene002546:intron 20.0%
!! TTTCAATCTATTACAAAATC+TGG - chr2.1:69969675-69969694 None:intergenic 20.0%
!!! AACAAAGAAGGTAAAATTTT+TGG - chr2.1:69972411-69972430 None:intergenic 20.0%
!!! AAGAAAATGTTTCCATTTTT+GGG - chr2.1:69972003-69972022 None:intergenic 20.0%
!!! TAAGAAAATGTTTCCATTTT+TGG - chr2.1:69972004-69972023 None:intergenic 20.0%
!!! TACTTTCTCAAAAATCATTT+TGG - chr2.1:69970872-69970891 None:intergenic 20.0%
!!! TGATTTTGTCATTGTTTTTA+GGG + chr2.1:69971939-69971958 MS.gene002546:intron 20.0%
!!! TTGATTTTGTCATTGTTTTT+AGG + chr2.1:69971938-69971957 MS.gene002546:intron 20.0%
!!! TTTGTTTTTTCTTTAGGTTT+AGG + chr2.1:69971651-69971670 MS.gene002546:intron 20.0%
! AAAACATGTGAAACAGATAT+AGG - chr2.1:69970728-69970747 None:intergenic 25.0%
! AAATTCCTTTCATTGATCTT+TGG + chr2.1:69972337-69972356 MS.gene002546:CDS 25.0%
! AGAAAAAACATGAAATGACA+TGG - chr2.1:69969636-69969655 None:intergenic 25.0%
! AGTGAACAAGAAATCTTTAA+AGG + chr2.1:69970410-69970429 MS.gene002546:intron 25.0%
! AGTTAATGAAACCTAATAAC+GGG + chr2.1:69971337-69971356 MS.gene002546:intron 25.0%
! CACTATGGAAAATATTTGTT+AGG + chr2.1:69969894-69969913 MS.gene002546:intron 25.0%
! GAAATGTGCAAATTTGATAT+TGG + chr2.1:69971421-69971440 MS.gene002546:intron 25.0%
! GAACTTATTAATTAGTGAGT+TGG + chr2.1:69971443-69971462 MS.gene002546:intron 25.0%
! GAGTTAATGAAACCTAATAA+CGG + chr2.1:69971336-69971355 MS.gene002546:intron 25.0%
! TAACAATGACTAATCTATGT+TGG - chr2.1:69970751-69970770 None:intergenic 25.0%
! TAAGAGAAGATAAACAAAGA+AGG - chr2.1:69972423-69972442 None:intergenic 25.0%
! TATCTTCTCTTACAATACTT+TGG + chr2.1:69972431-69972450 MS.gene002546:intron 25.0%
! TTACCAAGTTACCTTAAAAA+AGG - chr2.1:69971193-69971212 None:intergenic 25.0%
! TTATTTGTTTGTGAACAATC+CGG + chr2.1:69972239-69972258 MS.gene002546:CDS 25.0%
!! ATGCTATGGTATTATAGTTT+TGG + chr2.1:69972601-69972620 MS.gene002546:intron 25.0%
!! GGTGACATTTTACATTTTAA+TGG - chr2.1:69971547-69971566 None:intergenic 25.0%
!! TCATCAAAATTGACTTTGTA+TGG - chr2.1:69972559-69972578 None:intergenic 25.0%
!!! ACATTTCCCTTGTTTTTATT+AGG + chr2.1:69972135-69972154 MS.gene002546:intron 25.0%
!!! CTTTGAGTTTTTCATTTTAG+AGG - chr2.1:69971478-69971497 None:intergenic 25.0%
!!! TCAATTTTTAGTTCACACTA+TGG + chr2.1:69969879-69969898 MS.gene002546:intron 25.0%
!!! TTTCATGTTTTTTCTCTTGA+TGG + chr2.1:69969641-69969660 MS.gene002546:intron 25.0%
!!! TTTTGGAAAACATCTTTGAA+AGG + chr2.1:69970237-69970256 MS.gene002546:intron 25.0%
AAAAAACAGCAACATTCTTG+TGG - chr2.1:69970485-69970504 None:intergenic 30.0%
AAGAGGCTCAAATATCTTAT+CGG - chr2.1:69969841-69969860 None:intergenic 30.0%
AATCAAAGTATGTGATGCTA+TGG + chr2.1:69972587-69972606 MS.gene002546:intron 30.0%
ATAAGGACAACATTAGTTGA+CGG - chr2.1:69971858-69971877 None:intergenic 30.0%
ATCTTTGGACTAAAATGCAA+CGG + chr2.1:69972352-69972371 MS.gene002546:CDS 30.0%
ATTTGAGCCTCTTAAATATG+TGG + chr2.1:69969848-69969867 MS.gene002546:intron 30.0%
TACCTTAAAAAAGGAGACAA+TGG - chr2.1:69971184-69971203 None:intergenic 30.0%
TCACCGAAAAGATATATTTG+TGG - chr2.1:69969504-69969523 None:intergenic 30.0%
TGGATCAATATCAGCTAAAA+GGG - chr2.1:69972767-69972786 None:intergenic 30.0%
TGTTAACTTAAATGCAGTGA+CGG + chr2.1:69972640-69972659 MS.gene002546:intron 30.0%
TTAGTCCAAAGATCAATGAA+AGG - chr2.1:69972345-69972364 None:intergenic 30.0%
TTGATAGAATAAGACGTGTT+TGG - chr2.1:69970644-69970663 None:intergenic 30.0%
TTGGATCAATATCAGCTAAA+AGG - chr2.1:69972768-69972787 None:intergenic 30.0%
! AGGCCACAAATATATCTTTT+CGG + chr2.1:69969498-69969517 MS.gene002546:CDS 30.0%
! TGAAAAACAGAGCATCTATT+AGG - chr2.1:69971123-69971142 None:intergenic 30.0%
! TTGCTTTGACAGTTTTCTTT+GGG + chr2.1:69971079-69971098 MS.gene002546:CDS 30.0%
!!! TCTCCTTTTTTAAGGTAACT+TGG + chr2.1:69971187-69971206 MS.gene002546:intron 30.0%
AAACAGATATAGGCTGTGTT+TGG - chr2.1:69970718-69970737 None:intergenic 35.0%
AAATGAAGAGTTAAGGAGAC+CGG + chr2.1:69971279-69971298 MS.gene002546:intron 35.0%
AAATGACATGGAAACATGGT+TGG - chr2.1:69969624-69969643 None:intergenic 35.0%
AACCGTAATATATGACAACC+TGG + chr2.1:69970434-69970453 MS.gene002546:intron 35.0%
AAGAAAACTGTCAAAGCAAC+AGG - chr2.1:69971078-69971097 None:intergenic 35.0%
ACCCAGGTTGTCATATATTA+CGG - chr2.1:69970439-69970458 None:intergenic 35.0%
ACCGTAATATATGACAACCT+GGG + chr2.1:69970435-69970454 MS.gene002546:intron 35.0%
AGAGGCTCAAATATCTTATC+GGG - chr2.1:69969840-69969859 None:intergenic 35.0%
AGGGAATCAATTTGTGTTTG+AGG + chr2.1:69972681-69972700 MS.gene002546:CDS 35.0%
ATATATGACAACCTGGGTTA+TGG + chr2.1:69970441-69970460 MS.gene002546:intron 35.0%
CAAAAAACGCTATAAGCTAG+TGG + chr2.1:69970605-69970624 MS.gene002546:intron 35.0%
CATGAAATGACATGGAAACA+TGG - chr2.1:69969628-69969647 None:intergenic 35.0%
CATGTTAAGCAACCCAAAAA+TGG + chr2.1:69971988-69972007 MS.gene002546:intron 35.0%
CTCTTGACCACATATTTAAG+AGG - chr2.1:69969858-69969877 None:intergenic 35.0%
GAAAGGAATTTGACACTCAT+TGG - chr2.1:69972328-69972347 None:intergenic 35.0%
GATCAATATCAGCTAAAAGG+GGG - chr2.1:69972765-69972784 None:intergenic 35.0%
GATGGGAAAAAGTTTACTTG+AGG + chr2.1:69972382-69972401 MS.gene002546:CDS 35.0%
GGATCAATATCAGCTAAAAG+GGG - chr2.1:69972766-69972785 None:intergenic 35.0%
TATATGACAACCTGGGTTAT+GGG + chr2.1:69970442-69970461 MS.gene002546:intron 35.0%
TTGCTTAACATGTGCCTTAA+GGG - chr2.1:69971980-69971999 None:intergenic 35.0%
TTTACTAAACACACCCATAG+TGG - chr2.1:69969929-69969948 None:intergenic 35.0%
! CTTTTGAATTTCAGAAGCGA+TGG + chr2.1:69971832-69971851 MS.gene002546:intron 35.0%
! TAAGCTAGCTTATAGCTTGT+TGG - chr2.1:69970686-69970705 None:intergenic 35.0%
! TGCTTTGACAGTTTTCTTTG+GGG + chr2.1:69971080-69971099 MS.gene002546:CDS 35.0%
!! AAGTAAACTTTTTCCCATCC+AGG - chr2.1:69972381-69972400 None:intergenic 35.0%
!! AATCATTAACTTGTGCCTTG+AGG + chr2.1:69972102-69972121 MS.gene002546:intron 35.0%
!! AATGTTAACTTGTACCCTCA+AGG - chr2.1:69972120-69972139 None:intergenic 35.0%
!! AGTAAACTTTTTCCCATCCA+GGG - chr2.1:69972380-69972399 None:intergenic 35.0%
!! ATCATTAACTTGTGCCTTGA+GGG + chr2.1:69972103-69972122 MS.gene002546:intron 35.0%
!! GTTGCTTTGACAGTTTTCTT+TGG + chr2.1:69971078-69971097 MS.gene002546:CDS 35.0%
!!! CTCCATTGTCTCCTTTTTTA+AGG + chr2.1:69971179-69971198 MS.gene002546:CDS 35.0%
AAGTCGTGCTTCTTCATCTA+TGG - chr2.1:69971892-69971911 None:intergenic 40.0%
AATGCCTTCAGTGGATCATT+TGG - chr2.1:69972787-69972806 None:intergenic 40.0%
AGTCGTGCTTCTTCATCTAT+GGG - chr2.1:69971891-69971910 None:intergenic 40.0%
ATCAAATGAGCTCGTGTGAT+AGG + chr2.1:69970942-69970961 MS.gene002546:intron 40.0%
GTTGCTTAACATGTGCCTTA+AGG - chr2.1:69971981-69972000 None:intergenic 40.0%
GTTTGCATCTCACTAATGGA+GGG + chr2.1:69972662-69972681 MS.gene002546:CDS 40.0%
TACCGTACGAGAGAAATAGT+TGG - chr2.1:69969577-69969596 None:intergenic 40.0%
TAGGAGAGAGAATCCACTAT+GGG + chr2.1:69969913-69969932 MS.gene002546:intron 40.0%
TCGATCACTGAAGAATCCTT+TGG + chr2.1:69969525-69969544 MS.gene002546:CDS 40.0%
TGATCCAAATGATCCACTGA+AGG + chr2.1:69972780-69972799 MS.gene002546:CDS 40.0%
TGCTAAAGCATGCATTTCTG+TGG + chr2.1:69972302-69972321 MS.gene002546:CDS 40.0%
TGTTCCTCTTCATGAGTACA+AGG + chr2.1:69971146-69971165 MS.gene002546:CDS 40.0%
TTAGGAGAGAGAATCCACTA+TGG + chr2.1:69969912-69969931 MS.gene002546:intron 40.0%
TTGTCCTTGTACTCATGAAG+AGG - chr2.1:69971153-69971172 None:intergenic 40.0%
TTGTTTGTGAACAATCCGGA+GGG + chr2.1:69972243-69972262 MS.gene002546:CDS 40.0%
TTTGTTTGTGAACAATCCGG+AGG + chr2.1:69972242-69972261 MS.gene002546:CDS 40.0%
! ACTGAAGAATCCTTTGGTGT+TGG + chr2.1:69969531-69969550 MS.gene002546:CDS 40.0%
! AGGGTTTTTCCAGTATCACA+TGG + chr2.1:69971027-69971046 MS.gene002546:intron 40.0%
! GTTTTTCCAGTATCACATGG+CGG + chr2.1:69971030-69971049 MS.gene002546:intron 40.0%
! TAGATGAAGAAGCACGACTT+AGG + chr2.1:69971892-69971911 MS.gene002546:CDS 40.0%
! TGCCAACTATTTCTCTCGTA+CGG + chr2.1:69969572-69969591 MS.gene002546:CDS 40.0%
! TGTGAGTCTAGAATCTATGC+AGG + chr2.1:69972732-69972751 MS.gene002546:CDS 40.0%
!! CATGTTAACTTGTGCCCTTA+AGG + chr2.1:69971963-69971982 MS.gene002546:intron 40.0%
AAAAGGAGACAATGGAGCGA+AGG - chr2.1:69971176-69971195 None:intergenic 45.0%
ACTAAAATGCAACGGTTCCC+TGG + chr2.1:69972360-69972379 MS.gene002546:CDS 45.0%
ATCGTGGGTATAACACACGA+TGG + chr2.1:69970986-69971005 MS.gene002546:CDS 45.0%
CAAAGCAACAGGAGCAGTTT+CGG - chr2.1:69971067-69971086 None:intergenic 45.0%
CTATGGGAGGAGGAGTTATA+AGG - chr2.1:69971875-69971894 None:intergenic 45.0%
GAAAGGACAAATGAAGCTGC+TGG + chr2.1:69972273-69972292 MS.gene002546:CDS 45.0%
GACGGTTTGCATCTCACTAA+TGG + chr2.1:69972658-69972677 MS.gene002546:CDS 45.0%
GCAGCTTCATTTGTCCTTTC+AGG - chr2.1:69972273-69972292 None:intergenic 45.0%
GGTTTGCATCTCACTAATGG+AGG + chr2.1:69972661-69972680 MS.gene002546:CDS 45.0%
TCGTGGGTATAACACACGAT+GGG + chr2.1:69970987-69971006 MS.gene002546:CDS 45.0%
TGTTTGTGAACAATCCGGAG+GGG + chr2.1:69972244-69972263 MS.gene002546:CDS 45.0%
!! AATCCTTTGGTGTTGGAGGA+TGG + chr2.1:69969538-69969557 MS.gene002546:CDS 45.0%
!! ATCCTTTGGTGTTGGAGGAT+GGG + chr2.1:69969539-69969558 MS.gene002546:CDS 45.0%
!! GAAGAATCCTTTGGTGTTGG+AGG + chr2.1:69969534-69969553 MS.gene002546:CDS 45.0%
!! AATAAAATAAAATAGATAAA+AGG + chr2.1:69970516-69970535 MS.gene002546:intron 5.0%
!!! TATTTTTTAATTTCAATTAT+AGG + chr2.1:69971767-69971786 MS.gene002546:intron 5.0%
AAATGCAACGGTTCCCTGGA+TGG + chr2.1:69972364-69972383 MS.gene002546:CDS 50.0%
AATGCAACGGTTCCCTGGAT+GGG + chr2.1:69972365-69972384 MS.gene002546:CDS 50.0%
ACCTGGGTTATGGGTAAACG+TGG + chr2.1:69970451-69970470 MS.gene002546:intron 50.0%
AGGCTGATGTGGTGTTCAGA+GGG + chr2.1:69970962-69970981 MS.gene002546:intron 50.0%
CGTGCTTCTTCATCTATGGG+AGG - chr2.1:69971888-69971907 None:intergenic 50.0%
GCTTCTTCATCTATGGGAGG+AGG - chr2.1:69971885-69971904 None:intergenic 50.0%
TAGGCTGATGTGGTGTTCAG+AGG + chr2.1:69970961-69970980 MS.gene002546:intron 50.0%
TCCACGTTTACCCATAACCC+AGG - chr2.1:69970455-69970474 None:intergenic 50.0%
!! GGCACTGAAGGTGTTGGAAA+GGG + chr2.1:69971008-69971027 MS.gene002546:CDS 50.0%
!! TAACACACGATGGGCACTGA+AGG + chr2.1:69970996-69971015 MS.gene002546:CDS 50.0%
!! TCCTTTGGTGTTGGAGGATG+GGG + chr2.1:69969540-69969559 MS.gene002546:CDS 50.0%
!! TGGTGTTCAGAGGGTATCGT+GGG + chr2.1:69970971-69970990 MS.gene002546:CDS 50.0%
ACCCCATCCTCCAACACCAA+AGG - chr2.1:69969544-69969563 None:intergenic 55.0%
AGTATCACATGGCGGTGACG+AGG + chr2.1:69971038-69971057 MS.gene002546:intron 55.0%
GCTCGTGTGATAGGCTGATG+TGG + chr2.1:69970951-69970970 MS.gene002546:intron 55.0%
GTATCACATGGCGGTGACGA+GGG + chr2.1:69971039-69971058 MS.gene002546:intron 55.0%
TCGTCACCGCCATGTGATAC+TGG - chr2.1:69971039-69971058 None:intergenic 55.0%
!! ACGATGGGCACTGAAGGTGT+TGG + chr2.1:69971002-69971021 MS.gene002546:CDS 55.0%
!! GGGCACTGAAGGTGTTGGAA+AGG + chr2.1:69971007-69971026 MS.gene002546:CDS 55.0%
!! GTGGTGTTCAGAGGGTATCG+TGG + chr2.1:69970970-69970989 MS.gene002546:CDS 55.0%
ATCCGGAGGGGCTTCCTGAA+AGG + chr2.1:69972256-69972275 MS.gene002546:CDS 60.0%
GTCCTTTCAGGAAGCCCCTC+CGG - chr2.1:69972261-69972280 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 69969489 69972813 69969489 ID=MS.gene002546
chr2.1 mRNA 69969489 69972813 69969489 ID=MS.gene002546.t1;Parent=MS.gene002546
chr2.1 exon 69969489 69969593 69969489 ID=MS.gene002546.t1.exon1;Parent=MS.gene002546.t1
chr2.1 CDS 69969489 69969593 69969489 ID=cds.MS.gene002546.t1;Parent=MS.gene002546.t1
chr2.1 exon 69970964 69971029 69970964 ID=MS.gene002546.t1.exon2;Parent=MS.gene002546.t1
chr2.1 CDS 69970964 69971029 69970964 ID=cds.MS.gene002546.t1;Parent=MS.gene002546.t1
chr2.1 exon 69971060 69971200 69971060 ID=MS.gene002546.t1.exon3;Parent=MS.gene002546.t1
chr2.1 CDS 69971060 69971200 69971060 ID=cds.MS.gene002546.t1;Parent=MS.gene002546.t1
chr2.1 exon 69971846 69971913 69971846 ID=MS.gene002546.t1.exon4;Parent=MS.gene002546.t1
chr2.1 CDS 69971846 69971913 69971846 ID=cds.MS.gene002546.t1;Parent=MS.gene002546.t1
chr2.1 exon 69972239 69972403 69972239 ID=MS.gene002546.t1.exon5;Parent=MS.gene002546.t1
chr2.1 CDS 69972239 69972403 69972239 ID=cds.MS.gene002546.t1;Parent=MS.gene002546.t1
chr2.1 exon 69972657 69972813 69972657 ID=MS.gene002546.t1.exon6;Parent=MS.gene002546.t1
chr2.1 CDS 69972657 69972813 69972657 ID=cds.MS.gene002546.t1;Parent=MS.gene002546.t1
Gene Sequence

>MS.gene002546

ATGACTAAGAGGCCACAAATATATCTTTTCGGTGATTCGATCACTGAAGAATCCTTTGGTGTTGGAGGATGGGGTGCTTCTCTTGCCAACTATTTCTCTCGTACGGTAATAATTCATTCTTGCTTTAAGTGACCAACCATGTTTCCATGTCATTTCATGTTTTTTCTCTTGATGGTTATTTTTCCAGATTTTGTAATAGATTGAAATGAAAGTGATTTTAAATAAAAATAAACAATGTTTTTATTTTTCGTTATGGTTTACAAATCATTCAAGTTGAAAAACTATACATGTTAAATTAGATTTTCATTAATATGATTTAAAGAAAGATAGATTCAGCAATCGATTGATCCCGATAAGATATTTGAGCCTCTTAAATATGTGGTCAAGAGTTCAATTTTTAGTTCACACTATGGAAAATATTTGTTAGGAGAGAGAATCCACTATGGGTGTGTTTAGTAAAAATAAGCTATAAGCTAGCTGATAGCTGATAAGCTAGCTGATAGATGAAAAGCTAGCTTATAGCTGATGACTGATAGCTGATAGCTTATAGCTTAAAAGTTAGCTTATTAAAATTAAAAGTGTTTGGTAAAATTAGCTGTTGAAGTGACTGATAAATATAAATGACGTAAAAGGACGTGTTTGTTTAATTTTTAATATAAATCATATATATAATTTTTATTACTTAACGTATTTCTTTTTAAAGATTTAAATATATTACAAATTATTTTCATCTTTAAAATGATTTTTTTTTTGGAAAACATCTTTGAAAGGATAAATATATATGAATTCAATCGATATTTTCTATTCGACAAAACTTTTTTTTATGAATTTTATTTAACTAAATTTGCTTCACTGTTTATTGTAAATTTTTATAAAATTATTTATTATATGAATTATGAACAAGGTAAAAAAAACAAAAAAAGTGAACAAGAAATCTTTAAAGGAAACCGTAATATATGACAACCTGGGTTATGGGTAAACGTGGACAGTTTACCACAAGAATGTTGCTGTTTTTTTTATTATAAAAAATAAAATAAAATAGATAAAAGGTTAATTTTGGAAGAAAATATAAAAAGCTACAAGCTAAAAGCTCAAAAGCTACTTGAAATAGCTCTTCAAAAAACGCTATAAGCTAGTGGAAAAAGCTTTTTACCAAACACGTCTTATTCTATCAAAACAAGCTTATAAGCTAGTCCAACAAGCTATAAGCTAGCTTATTTGTGTTACCAAACACAGCCTATATCTGTTTCACATGTTTTCCAACATAGATTAGTCATTGTTAAACAACTTCATACTAATTTCATGTAACCAAAAATAATTTTTTTTTAATTTTCAAAAACTAATTAAAGTAATTGTTACATTTTCAAACTTTTTTTTTTACCAAAATGATTTTTGAGAAAGTACACACAAACATAATTAAAAGATCAAAAGTGATTTTCTCAAAATATTTTTAATCAAATGAGCTCGTGTGATAGGCTGATGTGGTGTTCAGAGGGTATCGTGGGTATAACACACGATGGGCACTGAAGGTGTTGGAAAGGGTTTTTCCAGTATCACATGGCGGTGACGAGGGAACCGAAACTGCTCCTGTTGCTTTGACAGTTTTCTTTGGGGCTAATGATGCTTGTCTTCCTAATAGATGCTCTGTTTTTCAACATGTTCCTCTTCATGAGTACAAGGACAACCTTCGCTCCATTGTCTCCTTTTTTAAGGTAACTTGGTAACAAGTTCTGATTTCTTCAATTATTTATTTTTGTTTATTATTGTTCATTCATAAGAGAGATAAACATAAATGAAGAGTTAAGGAGACCGGCTTTTAAAATAAGATTTTTTTTTTTTAAAATCAAGAGTTAATGAAACCTAATAACGGGTTTATTTATTAAAAAAAATCTATCATGTGCAACTTTGATATTAGAACTTTTTTTTTTGAAAGAAATGTGCAAATTTGATATTGGAACTTATTAATTAGTGAGTTGGGATCTGATTCCTCTAAAATGAAAAACTCAAAGTAAAATATTAAATCAAATGTAGAAATAGTAGGTTCATGCAAATCAACCATTAAAATGTAAAATGTCACCATTATATGATAAATCAAATGTAGAAATTGTAGGTTCATGCAAATCATAAGTTTGTTATTAACAACTCTTAACTAGCTTCTTAATTTTGTTTTTTCTTTAGGTTTAGGCTCTCTTTTGTTCATGAAAAAACAACTTTTTATTTTCTGACTTTACTCTTTAGCCACTTTAAATGTTTAGAATTTTGTTTGATGACGCTTAAATATTTTTTAATTTCAATTATAGGCCTCTTGTATAAATAAATCATATAATTGGTTACTGCAACTTTCTTTTGAATTTCAGAAGCGATGGCCGTCAACTAATGTTGTCCTTATAACTCCTCCTCCCATAGATGAAGAAGCACGACTTAGGTAATGTTTCATTGACTTTTTTCCTTGATTTTGTCATTGTTTTTAGGGTCATGTTAACTTGTGCCCTTAAGGCACATGTTAAGCAACCCAAAAATGGAAACATTTTCTTAAATTTTGTGCATTCAATTATTGAAAAGTAAAAAAGTTAAAGTCAAATGCATTAATTACAATAGAATATTTTTATTTTGAATCATTAACTTGTGCCTTGAGGGTACAAGTTAACATTTCCCTTGTTTTTATTAGGTTTTCTTTGTTTGTGACACACACACACGTATAATTCTATAGAAGTTAATCATACTTAGGAATTTCTAAAAATTTATTGTAGTTATTTGTTTGTGAACAATCCGGAGGGGCTTCCTGAAAGGACAAATGAAGCTGCTGGTGAATATGCTAAAGCATGCATTTCTGTGGCCAATGAGTGTCAAATTCCTTTCATTGATCTTTGGACTAAAATGCAACGGTTCCCTGGATGGGAAAAAGTTTACTTGAGGTTTCCAAAAATTTTACCTTCTTTGTTTATCTTCTCTTACAATACTTTGGAATCTTGATATTAGTATTTACCATTTAATTTAAAATACTAATGAACATGATGGCTTTGTTATAGTTTGATTAAATATGCAAAGATAATTGGAAAATCAATTACCATACAAAGTCAATTTTGATGATGCTAATTAATCAAAGTATGTGATGCTATGGTATTATAGTTTTGGTTTTGATATATGATTATGTTAACTTAAATGCAGTGACGGTTTGCATCTCACTAATGGAGGGAATCAATTTGTGTTTGAGGAAGTAATAAAAAAACTAAGAGATGAAGGTGTGAGTCTAGAATCTATGCAGGTTGATCTCCCCCTTTTAGCTGATATTGATCCAAATGATCCACTGAAGGCATTTTTGTAG
Protein sequence

>MS.gene002546.t1

MTKRPQIYLFGDSITEESFGVGGWGASLANYFSRTADVVFRGYRGYNTRWALKVLERGTETAPVALTVFFGANDACLPNRCSVFQHVPLHEYKDNLRSIVSFFKKRWPSTNVVLITPPPIDEEARLSYLFVNNPEGLPERTNEAAGEYAKACISVANECQIPFIDLWTKMQRFPGWEKVYLSDGLHLTNGGNQFVFEEVIKKLRDEGVSLESMQVDLPLLADIDPNDPLKAFL