AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004151

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004151.t1 MTR_4g461070 98.827 341 4 0 1 341 1 341 0.0 702
MS.gene004151.t1 MTR_1g064060 96.188 341 13 0 1 341 1 341 0.0 686
MS.gene004151.t1 MTR_1g064060 94.983 299 15 0 1 299 1 299 0.0 593
MS.gene004151.t1 MTR_3g112040 25.382 327 212 14 12 326 27 333 4.04e-12 67.0
MS.gene004151.t1 MTR_3g081400 61.364 44 14 1 263 306 34 74 6.21e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004151.t1 AT5G03300 83.383 337 56 0 4 340 8 344 0.0 605
MS.gene004151.t1 AT3G09820 83.728 338 55 0 3 340 6 343 0.0 601
MS.gene004151.t1 AT3G09820 85.034 294 44 0 47 340 8 301 0.0 529
MS.gene004151.t1 AT5G03300 83.849 291 47 0 50 340 1 291 0.0 525
MS.gene004151.t1 AT5G03300 83.849 291 47 0 50 340 1 291 0.0 525
MS.gene004151.t1 AT1G19600 25.602 332 218 13 14 336 23 334 6.09e-14 72.4

Find 90 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTATCTGTGAGTTCTTTA+AGG 0.167860 4.2:-43151092 MS.gene004151:CDS
ACCAGCTTGTTTAGAGTTCT+TGG 0.208302 4.2:+43151769 None:intergenic
ATTTGAAGAAACCCGAAAAT+TGG 0.225304 4.2:-43151416 MS.gene004151:CDS
CATTCTGGGATTCAGATATA+TGG 0.229738 4.2:-43150981 MS.gene004151:intron
TTCCTGATGCCTTTGGCAAC+TGG 0.236821 4.2:+43150774 None:intergenic
GCCAAATATTTCTATATTGC+TGG 0.248568 4.2:-43151284 MS.gene004151:CDS
TCTTTAGGTAATAGAATTAC+AGG 0.256176 4.2:+43150674 None:intergenic
CTTAAAGAACTCACAGATAA+AGG 0.274294 4.2:+43151093 None:intergenic
AATGTACTCCACGTTATATT+TGG 0.281042 4.2:+43152097 None:intergenic
TCCAGCAATATAGAAATATT+TGG 0.289493 4.2:+43151283 None:intergenic
TATTGAAAATCAGGCTTCTC+TGG 0.291194 4.2:+43150415 None:intergenic
GTATCAAGTAGTTTCTCTTT+AGG 0.298233 4.2:+43150659 None:intergenic
GCTTTGGAAGGTGTTCTTCT+TGG 0.324177 4.2:-43153247 MS.gene004151:CDS
AGAATCACTTATTGAAAATC+AGG 0.327042 4.2:+43150406 None:intergenic
TTTATCTGTGAGTTCTTTAA+GGG 0.329968 4.2:-43151091 MS.gene004151:CDS
AGCAACTAATTGAATGGATT+CGG 0.333100 4.2:+43151241 None:intergenic
GGAAGGTGTTCTTCTTGGTA+TGG 0.348012 4.2:-43153242 MS.gene004151:CDS
GCAACTAATTGAATGGATTC+GGG 0.354886 4.2:+43151242 None:intergenic
TAGTGGTTGCTTCAAGTTCC+CGG 0.356075 4.2:-43151845 MS.gene004151:CDS
TGGAGGATTTCTTTCACAAT+TGG 0.357365 4.2:-43150521 MS.gene004151:CDS
GCTCTCACACATTCTTCAAT+AGG 0.364927 4.2:+43150487 None:intergenic
TCCTGATGCCTTTGGCAACT+GGG 0.376131 4.2:+43150775 None:intergenic
CGAGAATAGCATTGTTCAAC+TGG 0.377377 4.2:+43153005 None:intergenic
TCTCGCATCAACAATGGCTT+TGG 0.379448 4.2:-43153263 None:intergenic
TTGTGTGTTCCTGATGCCTT+TGG 0.383545 4.2:+43150767 None:intergenic
TGCATTGGGAAGGACAAGTT+TGG 0.384984 4.2:-43151803 MS.gene004151:CDS
AAACCCGAAAATTGGGCATT+AGG 0.397247 4.2:-43151408 MS.gene004151:intron
TTTGAAGAAACCCGAAAATT+GGG 0.414245 4.2:-43151415 MS.gene004151:CDS
AGCTATCTCCTCGACATTAT+CGG 0.414959 4.2:+43150808 None:intergenic
ACTTGTGCTGTTTGTGTCGT+TGG 0.426149 4.2:-43151613 MS.gene004151:CDS
ACCGATGTAACTAGTGGCTC+CGG 0.432188 4.2:+43151826 None:intergenic
CTAGTTACATCGGTTGCATT+GGG 0.448977 4.2:-43151817 MS.gene004151:CDS
TTTGTAGGAGATGCCTTTGT+TGG 0.450937 4.2:-43150541 MS.gene004151:intron
ACTAGTTACATCGGTTGCAT+TGG 0.454698 4.2:-43151818 MS.gene004151:CDS
GTAGGAGATGCCTTTGTTGG+AGG 0.455368 4.2:-43150538 MS.gene004151:intron
TTAAAGAACTCACAGATAAA+GGG 0.458960 4.2:+43151094 None:intergenic
TCCCAGTTGCCAAAGGCATC+AGG 0.461050 4.2:-43150776 MS.gene004151:CDS
ATTCTTCAATAGGTTTCTCC+TGG 0.464884 4.2:+43150497 None:intergenic
GTGCTACTCAGAATTCAATC+AGG 0.465309 4.2:-43151973 MS.gene004151:CDS
GAAGGTGTTCTTCTTGGTAT+GGG 0.471521 4.2:-43153241 MS.gene004151:CDS
GAATTCAATCAGGGTTGCTC+AGG 0.472160 4.2:-43151963 MS.gene004151:intron
TAGGGCAATACCTGCTCCAT+TGG 0.477788 4.2:+43150637 None:intergenic
ATGTTCAGCAACTAATTGAA+TGG 0.478120 4.2:+43151235 None:intergenic
GCAATTTGCAGCTGATAAGT+TGG 0.489440 4.2:+43151452 None:intergenic
CTTCTCTGGGTAAGTGCAGC+CGG 0.491076 4.2:+43150429 None:intergenic
ATTGAAAATCAGGCTTCTCT+GGG 0.503018 4.2:+43150416 None:intergenic
ATTTCTTTCACAATTGGTCC+AGG 0.505204 4.2:-43150515 MS.gene004151:CDS
AATGTTATCATTCAAAGGTC+CGG 0.511083 4.2:-43150448 MS.gene004151:CDS
CCTGTGTGTGTTGCTCAAGA+TGG 0.511785 4.2:-43150713 MS.gene004151:CDS
TATAACGTGGAGTACATTGC+TGG 0.514705 4.2:-43152092 MS.gene004151:CDS
ATCAGCACCCTGTGTGATAA+CGG 0.520980 4.2:+43150736 None:intergenic
AGGCATCAGGAACACACAAA+AGG 0.529385 4.2:-43150763 MS.gene004151:CDS
TGAGTTCTTTAAGGGTCCAC+AGG 0.534924 4.2:-43151083 MS.gene004151:CDS
TACGGAAGAGCTTTCTCCTG+TGG 0.542115 4.2:+43151067 None:intergenic
TTTGTGTCGTTGGTGGTGAA+AGG 0.544058 4.2:-43151603 MS.gene004151:intron
GAGAAACTACTTGATACCAA+TGG 0.548375 4.2:-43150653 MS.gene004151:CDS
CTACTTGATACCAATGGAGC+AGG 0.549364 4.2:-43150647 MS.gene004151:intron
TGTGCTGTTTGTGTCGTTGG+TGG 0.549931 4.2:-43151610 MS.gene004151:CDS
CAGCAAATGTTATCATTCAA+AGG 0.551386 4.2:-43150453 MS.gene004151:CDS
ATTGAAGAATGTGTGAGAGC+TGG 0.554569 4.2:-43150484 MS.gene004151:CDS
CTGTGTGTGTTGCTCAAGAT+GGG 0.562754 4.2:-43150712 MS.gene004151:CDS
GACAGCAGAAATGTCGAGAA+GGG 0.565725 4.2:+43153213 None:intergenic
CGACAGCAGAAATGTCGAGA+AGG 0.566798 4.2:+43153212 None:intergenic
TTGAAGAATGTGTGAGAGCT+GGG 0.569874 4.2:-43150483 MS.gene004151:CDS
GAAGACGAGAATACACCTAC+AGG 0.574426 4.2:-43151637 MS.gene004151:CDS
ACCAAGAACTCTAAACAAGC+TGG 0.574572 4.2:-43151770 MS.gene004151:CDS
TGAAAGAAATCCTCCAACAA+AGG 0.578951 4.2:+43150528 None:intergenic
CACAGATAAAGGGTGCAGAA+AGG 0.579545 4.2:+43151104 None:intergenic
CCGATGTAACTAGTGGCTCC+GGG 0.591002 4.2:+43151827 None:intergenic
CCCGGAGCCACTAGTTACAT+CGG 0.598586 4.2:-43151827 MS.gene004151:CDS
TTACATCGGTTGCATTGGGA+AGG 0.601422 4.2:-43151813 MS.gene004151:CDS
CTTTAGGTAATAGAATTACA+GGG 0.605211 4.2:+43150675 None:intergenic
GCATCAACAATGGCTTTGGA+AGG 0.614210 4.2:-43153259 None:intergenic
CAAGATATCCCAGTTGCCAA+AGG 0.619346 4.2:-43150783 MS.gene004151:CDS
AAGGATTACCGTTATCACAC+AGG 0.620728 4.2:-43150744 MS.gene004151:CDS
AACTAATTGAATGGATTCGG+GGG 0.625445 4.2:+43151244 None:intergenic
TGCTACTCAGAATTCAATCA+GGG 0.630239 4.2:-43151972 MS.gene004151:CDS
CCATCTTGAGCAACACACAC+AGG 0.632679 4.2:+43150713 None:intergenic
TGGGAAGGACAAGTTTGGCG+AGG 0.639413 4.2:-43151798 MS.gene004151:CDS
CAACTAATTGAATGGATTCG+GGG 0.644425 4.2:+43151243 None:intergenic
ACATGCAGCTGCAAATAACA+AGG 0.645685 4.2:-43151216 MS.gene004151:intron
ACAGCAGAAATGTCGAGAAG+GGG 0.652637 4.2:+43153214 None:intergenic
CAAACAGCACAAGTTCCTGT+AGG 0.659898 4.2:+43151622 None:intergenic
TTCGAAAGTTCATGGCTGGG+AGG 0.661884 4.2:-43150921 MS.gene004151:intron
TTGGAAATGAGACTGAAGCA+CGG 0.674931 4.2:-43150949 MS.gene004151:CDS
TGACCAGACCGATAATGTCG+AGG 0.675199 4.2:-43150816 MS.gene004151:intron
AATGCAACCGATGTAACTAG+TGG 0.675466 4.2:+43151820 None:intergenic
AGGATTACCGTTATCACACA+GGG 0.685783 4.2:-43150743 MS.gene004151:CDS
AACGTGGAGTACATTGCTGG+AGG 0.692110 4.2:-43152089 MS.gene004151:intron
AATGACTGCCAAATATAACG+TGG 0.704099 4.2:-43152105 MS.gene004151:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAATGAATGATTTTT+GGG + chr4.2:43152120-43152139 None:intergenic 10.0%
!!! TAAAAAAAATGAATGATTTT+TGG + chr4.2:43152121-43152140 None:intergenic 10.0%
!!! TTTTAAATAAATAGTTGAAA+AGG - chr4.2:43152358-43152377 MS.gene004151:intron 10.0%
!! AACTTATTTATTTAACAACT+AGG - chr4.2:43151993-43152012 MS.gene004151:intron 15.0%
!! AATCATTTATTTGAATTTGA+CGG + chr4.2:43152634-43152653 None:intergenic 15.0%
!!! ATTTTGATGTAATTATTTCT+TGG - chr4.2:43151011-43151030 MS.gene004151:intron 15.0%
!!! CACTTTTCATTTTTAAATTA+CGG - chr4.2:43151073-43151092 MS.gene004151:CDS 15.0%
!!! TAAATTTTTTTCTTTTTTGC+AGG - chr4.2:43151669-43151688 MS.gene004151:intron 15.0%
!!! TACTTTTCCTTTTTAAATTA+CGG + chr4.2:43151202-43151221 None:intergenic 15.0%
!!! TCACTTTCTAAAAAAAATAT+TGG + chr4.2:43151341-43151360 None:intergenic 15.0%
!!! TTTTATTTAATAACTCATCT+TGG - chr4.2:43152813-43152832 MS.gene004151:intron 15.0%
!! AAATAAGTTAATTTCAGAAG+TGG + chr4.2:43151982-43152001 None:intergenic 20.0%
!! ATATAGTCCGTAATTTAAAA+AGG - chr4.2:43151192-43151211 MS.gene004151:intron 20.0%
!! TCTAAAAAAAATATTGGTGT+CGG + chr4.2:43151335-43151354 None:intergenic 20.0%
!! TTTAGTTAACATGCTAATTT+AGG - chr4.2:43150762-43150781 MS.gene004151:CDS 20.0%
!!! ATTCATTTTTTTTACGTCTT+TGG - chr4.2:43152127-43152146 MS.gene004151:intron 20.0%
!!! TGTGCTACAATATATATTTT+AGG - chr4.2:43150926-43150945 MS.gene004151:CDS 20.0%
!!! TTCTTTTGTTTTGTTTTTGT+AGG - chr4.2:43153108-43153127 MS.gene004151:intron 20.0%
! AAAAAGATCCTGAATTAAGA+AGG + chr4.2:43152284-43152303 None:intergenic 25.0%
! AAGAAGAAGAAGAAGAATAA+TGG - chr4.2:43150558-43150577 MS.gene004151:intron 25.0%
! CAAATTCTCAAAGATCAATA+AGG + chr4.2:43152663-43152682 None:intergenic 25.0%
! CATAGATACAAAAACACAAA+GGG + chr4.2:43151269-43151288 None:intergenic 25.0%
! CTTTAGGTAATAGAATTACA+GGG + chr4.2:43152992-43153011 None:intergenic 25.0%
! TCATAGATACAAAAACACAA+AGG + chr4.2:43151270-43151289 None:intergenic 25.0%
! TCATCAAAATGACTAACATT+TGG - chr4.2:43152324-43152343 MS.gene004151:intron 25.0%
! TCCAGCAATATAGAAATATT+TGG + chr4.2:43152384-43152403 None:intergenic 25.0%
! TCTTTAGGTAATAGAATTAC+AGG + chr4.2:43152993-43153012 None:intergenic 25.0%
! TTAAAGAACTCACAGATAAA+GGG + chr4.2:43152573-43152592 None:intergenic 25.0%
! TTTATCTGTGAGTTCTTTAA+GGG - chr4.2:43152573-43152592 MS.gene004151:intron 25.0%
! TTTGTATTTGCTTCTTATAG+TGG - chr4.2:43151802-43151821 MS.gene004151:CDS 25.0%
!! TCAGTTTAGCTTATTTACTT+TGG - chr4.2:43150956-43150975 MS.gene004151:CDS 25.0%
!! TTTTAGATTATTGCGTTTCT+GGG - chr4.2:43151616-43151635 MS.gene004151:CDS 25.0%
!! TTTTTAGATTATTGCGTTTC+TGG - chr4.2:43151615-43151634 MS.gene004151:CDS 25.0%
!!! AGATATATGGACTATGTTTT+TGG - chr4.2:43152696-43152715 MS.gene004151:intron 25.0%
!!! ATTTTGGTAGTTTTAACGAT+TGG - chr4.2:43150873-43150892 MS.gene004151:intron 25.0%
AAAAGAAGGTTTCAAAGAAC+TGG + chr4.2:43152168-43152187 None:intergenic 30.0%
AAAGAAGGTTTCAAAGAACT+GGG + chr4.2:43152167-43152186 None:intergenic 30.0%
AATGTAAAACCAAAACTGCA+AGG + chr4.2:43152795-43152814 None:intergenic 30.0%
AATGTACTCCACGTTATATT+TGG + chr4.2:43151570-43151589 None:intergenic 30.0%
AATGTTATCATTCAAAGGTC+CGG - chr4.2:43153216-43153235 MS.gene004151:CDS 30.0%
ACACACCCTAAATAGAAAAA+TGG + chr4.2:43151037-43151056 None:intergenic 30.0%
ATGTAAAACCAAAACTGCAA+GGG + chr4.2:43152794-43152813 None:intergenic 30.0%
ATGTAATTACAAGTGTCAGT+CGG - chr4.2:43151370-43151389 MS.gene004151:intron 30.0%
ATGTTCAGCAACTAATTGAA+TGG + chr4.2:43152432-43152451 None:intergenic 30.0%
ATTAGGTACCTTCTTAATTC+AGG - chr4.2:43152273-43152292 MS.gene004151:intron 30.0%
ATTCAACTTCAGAAAGCAAT+AGG + chr4.2:43153049-43153068 None:intergenic 30.0%
ATTTGAAGAAACCCGAAAAT+TGG - chr4.2:43152248-43152267 MS.gene004151:intron 30.0%
CAGCAAATGTTATCATTCAA+AGG - chr4.2:43153211-43153230 MS.gene004151:CDS 30.0%
CTTAAAGAACTCACAGATAA+AGG + chr4.2:43152574-43152593 None:intergenic 30.0%
CTTTATCTGTGAGTTCTTTA+AGG - chr4.2:43152572-43152591 MS.gene004151:intron 30.0%
GTATCAAGTAGTTTCTCTTT+AGG + chr4.2:43153008-43153027 None:intergenic 30.0%
TATCAAATACTTCTAGTCGA+AGG + chr4.2:43152500-43152519 None:intergenic 30.0%
TTCAACTTCAGAAAGCAATA+GGG + chr4.2:43153048-43153067 None:intergenic 30.0%
TTTGAAGAAACCCGAAAATT+GGG - chr4.2:43152249-43152268 MS.gene004151:intron 30.0%
! AAAAACACAAAGGGTTTGTT+TGG + chr4.2:43151260-43151279 None:intergenic 30.0%
! AATTTCAGAAGTGGATTCTA+CGG + chr4.2:43151973-43151992 None:intergenic 30.0%
! AGCAACTAATTGAATGGATT+CGG + chr4.2:43152426-43152445 None:intergenic 30.0%
! ATCTTTGAGAATTTGCATTC+TGG - chr4.2:43152668-43152687 MS.gene004151:intron 30.0%
! GCCAAATATTTCTATATTGC+TGG - chr4.2:43152380-43152399 MS.gene004151:intron 30.0%
! TCTTTGAGAATTTGCATTCT+GGG - chr4.2:43152669-43152688 MS.gene004151:intron 30.0%
!! TTGCTTTCTGAAGTTGAATA+TGG - chr4.2:43153050-43153069 MS.gene004151:intron 30.0%
!! TTTTCTATTTAGGGTGTGTT+TGG - chr4.2:43151038-43151057 MS.gene004151:intron 30.0%
!! TTTTGGGCATTTAAAACACA+AGG + chr4.2:43152104-43152123 None:intergenic 30.0%
!!! CGAGTTTAGCTTATTGATTT+TGG - chr4.2:43150857-43150876 MS.gene004151:intron 30.0%
!!! CTTGGCCATTTTTCTATTTA+GGG - chr4.2:43151029-43151048 MS.gene004151:intron 30.0%
!!! TCTTGGCCATTTTTCTATTT+AGG - chr4.2:43151028-43151047 MS.gene004151:intron 30.0%
AATACTTCTAGTCGAAGGTT+AGG + chr4.2:43152495-43152514 None:intergenic 35.0%
AATGACTGCCAAATATAACG+TGG - chr4.2:43151559-43151578 MS.gene004151:intron 35.0%
ATTCTTCAATAGGTTTCTCC+TGG + chr4.2:43153170-43153189 None:intergenic 35.0%
ATTGAAAATCAGGCTTCTCT+GGG + chr4.2:43153251-43153270 None:intergenic 35.0%
ATTTCTTTCACAATTGGTCC+AGG - chr4.2:43153149-43153168 MS.gene004151:intron 35.0%
CAAGATTGCACAATCATTTC+AGG - chr4.2:43151528-43151547 MS.gene004151:intron 35.0%
CCTACATGCAATCAAAAAGA+AGG + chr4.2:43152182-43152201 None:intergenic 35.0%
GAGAAACTACTTGATACCAA+TGG - chr4.2:43153011-43153030 MS.gene004151:CDS 35.0%
GTATGCTAAATTCTTTGCGA+TGG - chr4.2:43151462-43151481 MS.gene004151:CDS 35.0%
TATTGAAAATCAGGCTTCTC+TGG + chr4.2:43153252-43153271 None:intergenic 35.0%
TGAAAGAAATCCTCCAACAA+AGG + chr4.2:43153139-43153158 None:intergenic 35.0%
TGCTACTCAGAATTCAATCA+GGG - chr4.2:43151692-43151711 MS.gene004151:intron 35.0%
TGGAGGATTTCTTTCACAAT+TGG - chr4.2:43153143-43153162 MS.gene004151:intron 35.0%
TTGACGGTGAAAGATTCTTA+CGG + chr4.2:43152618-43152637 None:intergenic 35.0%
TTGGATTGAGGGTTTATGAA+GGG + chr4.2:43151241-43151260 None:intergenic 35.0%
TTTGGATTGAGGGTTTATGA+AGG + chr4.2:43151242-43151261 None:intergenic 35.0%
! AAAGGGTTTGTTTGGATTGA+GGG + chr4.2:43151252-43151271 None:intergenic 35.0%
! AACTAATTGAATGGATTCGG+GGG + chr4.2:43152423-43152442 None:intergenic 35.0%
! ATTTTCGAAAGTTCATGGCT+GGG - chr4.2:43152740-43152759 MS.gene004151:intron 35.0%
! CAACTAATTGAATGGATTCG+GGG + chr4.2:43152424-43152443 None:intergenic 35.0%
! GCAACTAATTGAATGGATTC+GGG + chr4.2:43152425-43152444 None:intergenic 35.0%
! TATTTAGGGTGTGTTTGGAT+GGG - chr4.2:43151043-43151062 MS.gene004151:intron 35.0%
!! CATTCTGGGATTCAGATATA+TGG - chr4.2:43152683-43152702 MS.gene004151:intron 35.0%
!! CCTTCTTTTTGATTGCATGT+AGG - chr4.2:43152179-43152198 MS.gene004151:intron 35.0%
!! TTGAGTCTTGTTTGCTATCA+AGG - chr4.2:43150983-43151002 MS.gene004151:intron 35.0%
!!! TGCTTGTTTTGGTTGTTTAC+AGG - chr4.2:43150628-43150647 MS.gene004151:intron 35.0%
AAACCCGAAAATTGGGCATT+AGG - chr4.2:43152256-43152275 MS.gene004151:intron 40.0%
AAGGATTACCGTTATCACAC+AGG - chr4.2:43152920-43152939 MS.gene004151:intron 40.0%
AATGCAACCGATGTAACTAG+TGG + chr4.2:43151847-43151866 None:intergenic 40.0%
ACATGCAGCTGCAAATAACA+AGG - chr4.2:43152448-43152467 MS.gene004151:intron 40.0%
ACATTATCGGTCTGGTCAAT+AGG + chr4.2:43152846-43152865 None:intergenic 40.0%
ACCAAGAACTCTAAACAAGC+TGG - chr4.2:43151894-43151913 MS.gene004151:intron 40.0%
AGCTATCTCCTCGACATTAT+CGG + chr4.2:43152859-43152878 None:intergenic 40.0%
AGCTGAATCTTGAGTACTTG+TGG - chr4.2:43151761-43151780 MS.gene004151:intron 40.0%
AGGATTACCGTTATCACACA+GGG - chr4.2:43152921-43152940 MS.gene004151:intron 40.0%
ATTGAAGAATGTGTGAGAGC+TGG - chr4.2:43153180-43153199 MS.gene004151:intron 40.0%
ATTGAGGGTTTATGAAGGGA+AGG + chr4.2:43151237-43151256 None:intergenic 40.0%
CATCATGAAAACCTGCACAA+GGG + chr4.2:43152538-43152557 None:intergenic 40.0%
CGAGAATAGCATTGTTCAAC+TGG + chr4.2:43150662-43150681 None:intergenic 40.0%
GCAATTTGCAGCTGATAAGT+TGG + chr4.2:43152215-43152234 None:intergenic 40.0%
GCTCTCACACATTCTTCAAT+AGG + chr4.2:43153180-43153199 None:intergenic 40.0%
TATAACGTGGAGTACATTGC+TGG - chr4.2:43151572-43151591 MS.gene004151:intron 40.0%
TCATCATGAAAACCTGCACA+AGG + chr4.2:43152539-43152558 None:intergenic 40.0%
TGAAGCACAAACATAGACAC+CGG - chr4.2:43151287-43151306 MS.gene004151:CDS 40.0%
TTGAAGAATGTGTGAGAGCT+GGG - chr4.2:43153181-43153200 MS.gene004151:intron 40.0%
TTGAGGGTTTATGAAGGGAA+GGG + chr4.2:43151236-43151255 None:intergenic 40.0%
TTGGAAATGAGACTGAAGCA+CGG - chr4.2:43152715-43152734 MS.gene004151:intron 40.0%
TTTGTAGGAGATGCCTTTGT+TGG - chr4.2:43153123-43153142 MS.gene004151:intron 40.0%
! ACCAGCTTGTTTAGAGTTCT+TGG + chr4.2:43151898-43151917 None:intergenic 40.0%
! ACTAGTTACATCGGTTGCAT+TGG - chr4.2:43151846-43151865 MS.gene004151:intron 40.0%
! AGATTATTGCGTTTCTGGGA+AGG - chr4.2:43151620-43151639 MS.gene004151:CDS 40.0%
! AGTATTTTACTCCCTTGTGC+AGG - chr4.2:43152524-43152543 MS.gene004151:intron 40.0%
! ATTTAGGGTGTGTTTGGATG+GGG - chr4.2:43151044-43151063 MS.gene004151:intron 40.0%
! CAAAGGGTTTGTTTGGATTG+AGG + chr4.2:43151253-43151272 None:intergenic 40.0%
! CATTTTCGAAAGTTCATGGC+TGG - chr4.2:43152739-43152758 MS.gene004151:intron 40.0%
! CGGACATTTTCGAAAGTTCA+TGG - chr4.2:43152735-43152754 MS.gene004151:intron 40.0%
! CTAGTTACATCGGTTGCATT+GGG - chr4.2:43151847-43151866 MS.gene004151:intron 40.0%
! CTATTTAGGGTGTGTTTGGA+TGG - chr4.2:43151042-43151061 MS.gene004151:intron 40.0%
! GCACTGACACTTATGATTGA+AGG + chr4.2:43151431-43151450 None:intergenic 40.0%
! GGTACCTAATGCCCAATTTT+CGG + chr4.2:43152263-43152282 None:intergenic 40.0%
! GTACCTAATGCCCAATTTTC+GGG + chr4.2:43152262-43152281 None:intergenic 40.0%
! GTGCTACTCAGAATTCAATC+AGG - chr4.2:43151691-43151710 MS.gene004151:intron 40.0%
! TCTCATGCTTCTGCTTGTTT+TGG - chr4.2:43150617-43150636 MS.gene004151:intron 40.0%
! TTATGATTGAAGGTGTGTCC+AGG + chr4.2:43151421-43151440 None:intergenic 40.0%
!! GAAGGTGTTCTTCTTGGTAT+GGG - chr4.2:43150423-43150442 MS.gene004151:CDS 40.0%
ACAGCAGAAATGTCGAGAAG+GGG + chr4.2:43150453-43150472 None:intergenic 45.0%
AGGCATCAGGAACACACAAA+AGG - chr4.2:43152901-43152920 MS.gene004151:intron 45.0%
ATCAGCACCCTGTGTGATAA+CGG + chr4.2:43152931-43152950 None:intergenic 45.0%
CAAACAGCACAAGTTCCTGT+AGG + chr4.2:43152045-43152064 None:intergenic 45.0%
CAAGATATCCCAGTTGCCAA+AGG - chr4.2:43152881-43152900 MS.gene004151:intron 45.0%
CTACTTGATACCAATGGAGC+AGG - chr4.2:43153017-43153036 MS.gene004151:CDS 45.0%
GAAGACGAGAATACACCTAC+AGG - chr4.2:43152027-43152046 MS.gene004151:intron 45.0%
GAATTCAATCAGGGTTGCTC+AGG - chr4.2:43151701-43151720 MS.gene004151:intron 45.0%
GACAGCAGAAATGTCGAGAA+GGG + chr4.2:43150454-43150473 None:intergenic 45.0%
TGCATTGGGAAGGACAAGTT+TGG - chr4.2:43151861-43151880 MS.gene004151:intron 45.0%
TTACAAGTGTCAGTCGGTGT+CGG - chr4.2:43151376-43151395 MS.gene004151:intron 45.0%
TTGTGTGTTCCTGATGCCTT+TGG + chr4.2:43152900-43152919 None:intergenic 45.0%
! CACAGATAAAGGGTGCAGAA+AGG + chr4.2:43152563-43152582 None:intergenic 45.0%
! CTGTGTGTGTTGCTCAAGAT+GGG - chr4.2:43152952-43152971 MS.gene004151:intron 45.0%
! GATCTGTACCCTTGCAGTTT+TGG - chr4.2:43152783-43152802 MS.gene004151:intron 45.0%
! TAGTGGTTGCTTCAAGTTCC+CGG - chr4.2:43151819-43151838 MS.gene004151:CDS 45.0%
! TGAGTTCTTTAAGGGTCCAC+AGG - chr4.2:43152581-43152600 MS.gene004151:intron 45.0%
! TTACATCGGTTGCATTGGGA+AGG - chr4.2:43151851-43151870 MS.gene004151:intron 45.0%
!! ACTTGTGCTGTTTGTGTCGT+TGG - chr4.2:43152051-43152070 MS.gene004151:intron 45.0%
!! GCTTTGGAAGGTGTTCTTCT+TGG - chr4.2:43150417-43150436 MS.gene004151:CDS 45.0%
!! GGAAGGTGTTCTTCTTGGTA+TGG - chr4.2:43150422-43150441 MS.gene004151:CDS 45.0%
!! TTTGTGTCGTTGGTGGTGAA+AGG - chr4.2:43152061-43152080 MS.gene004151:intron 45.0%
AACGTGGAGTACATTGCTGG+AGG - chr4.2:43151575-43151594 MS.gene004151:intron 50.0%
ACCGATGTAACTAGTGGCTC+CGG + chr4.2:43151841-43151860 None:intergenic 50.0%
AGGGTTTATGAAGGGAAGGG+AGG + chr4.2:43151233-43151252 None:intergenic 50.0%
CCATCTTGAGCAACACACAC+AGG + chr4.2:43152954-43152973 None:intergenic 50.0%
CGACAGCAGAAATGTCGAGA+AGG + chr4.2:43150455-43150474 None:intergenic 50.0%
GAGTGTCAGTGTTAGTGTCG+TGG + chr4.2:43151313-43151332 None:intergenic 50.0%
GGGTTTATGAAGGGAAGGGA+GGG + chr4.2:43151232-43151251 None:intergenic 50.0%
GGTTTATGAAGGGAAGGGAG+GGG + chr4.2:43151231-43151250 None:intergenic 50.0%
GTAGGAGATGCCTTTGTTGG+AGG - chr4.2:43153126-43153145 MS.gene004151:intron 50.0%
TACGGAAGAGCTTTCTCCTG+TGG + chr4.2:43152600-43152619 None:intergenic 50.0%
TAGGGCAATACCTGCTCCAT+TGG + chr4.2:43153030-43153049 None:intergenic 50.0%
TCCTGATGCCTTTGGCAACT+GGG + chr4.2:43152892-43152911 None:intergenic 50.0%
TCTCCTCGACATTATCGGTC+TGG + chr4.2:43152854-43152873 None:intergenic 50.0%
TGACCAGACCGATAATGTCG+AGG - chr4.2:43152848-43152867 MS.gene004151:intron 50.0%
TTCCTGATGCCTTTGGCAAC+TGG + chr4.2:43152893-43152912 None:intergenic 50.0%
TTCGAAAGTTCATGGCTGGG+AGG - chr4.2:43152743-43152762 MS.gene004151:intron 50.0%
! CAGAAGTGGATTCTACGGTC+TGG + chr4.2:43151968-43151987 None:intergenic 50.0%
! CCTGTGTGTGTTGCTCAAGA+TGG - chr4.2:43152951-43152970 MS.gene004151:intron 50.0%
!! TGTGCTGTTTGTGTCGTTGG+TGG - chr4.2:43152054-43152073 MS.gene004151:intron 50.0%
CATGACACGTGTCAGACACC+TGG - chr4.2:43151400-43151419 MS.gene004151:intron 55.0%
CCCGGAGCCACTAGTTACAT+CGG - chr4.2:43151837-43151856 MS.gene004151:CDS 55.0%
CCGATGTAACTAGTGGCTCC+GGG + chr4.2:43151840-43151859 None:intergenic 55.0%
CTTCTCTGGGTAAGTGCAGC+CGG + chr4.2:43153238-43153257 None:intergenic 55.0%
GTCAGTGTTAGTGTCGTGGC+CGG + chr4.2:43151309-43151328 None:intergenic 55.0%
TGGATTCGGGGGATACAGTG+AGG + chr4.2:43152412-43152431 None:intergenic 55.0%
TGGGAAGGACAAGTTTGGCG+AGG - chr4.2:43151866-43151885 MS.gene004151:intron 55.0%
!! CGTTGGTGGTGAAAGGTGAG+AGG - chr4.2:43152068-43152087 MS.gene004151:intron 55.0%
!! TCCCAGTTGCCAAAGGCATC+AGG - chr4.2:43152888-43152907 MS.gene004151:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 43150414 43153272 43150414 ID=MS.gene004151
chr4.2 mRNA 43150414 43153272 43150414 ID=MS.gene004151.t1;Parent=MS.gene004151
chr4.2 exon 43153190 43153272 43153190 ID=MS.gene004151.t1.exon1;Parent=MS.gene004151.t1
chr4.2 CDS 43153190 43153272 43153190 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43152983 43153036 43152983 ID=MS.gene004151.t1.exon2;Parent=MS.gene004151.t1
chr4.2 CDS 43152983 43153036 43152983 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43152090 43152136 43152090 ID=MS.gene004151.t1.exon3;Parent=MS.gene004151.t1
chr4.2 CDS 43152090 43152136 43152090 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43151964 43151995 43151964 ID=MS.gene004151.t1.exon4;Parent=MS.gene004151.t1
chr4.2 CDS 43151964 43151995 43151964 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43151763 43151864 43151763 ID=MS.gene004151.t1.exon5;Parent=MS.gene004151.t1
chr4.2 CDS 43151763 43151864 43151763 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43151604 43151671 43151604 ID=MS.gene004151.t1.exon6;Parent=MS.gene004151.t1
chr4.2 CDS 43151604 43151671 43151604 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43151409 43151485 43151409 ID=MS.gene004151.t1.exon7;Parent=MS.gene004151.t1
chr4.2 CDS 43151409 43151485 43151409 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43151217 43151314 43151217 ID=MS.gene004151.t1.exon8;Parent=MS.gene004151.t1
chr4.2 CDS 43151217 43151314 43151217 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43151070 43151140 43151070 ID=MS.gene004151.t1.exon9;Parent=MS.gene004151.t1
chr4.2 CDS 43151070 43151140 43151070 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43150922 43150988 43150922 ID=MS.gene004151.t1.exon10;Parent=MS.gene004151.t1
chr4.2 CDS 43150922 43150988 43150922 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43150648 43150831 43150648 ID=MS.gene004151.t1.exon11;Parent=MS.gene004151.t1
chr4.2 CDS 43150648 43150831 43150648 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
chr4.2 exon 43150414 43150556 43150414 ID=MS.gene004151.t1.exon12;Parent=MS.gene004151.t1
chr4.2 CDS 43150414 43150556 43150414 ID=cds.MS.gene004151.t1;Parent=MS.gene004151.t1
Gene Sequence

>MS.gene004151

ATGGCTTTGGAAGGTGTTCTTCTTGGTATGGGTAATCCCCTTCTCGACATTTCTGCTGTCGTTGATGAAGATTTCTTGAAAAAGTACGTCATTTTTATCTTCTTTCTTTTTATTTTTTAGTATATTGTTTTAGTTTGAATGAAGAAGAAGAAGAAGAAGAATAATGGTGTTGATGATTTGATTAAAAAAAATTAAAAATCAGATCTCATGCTTCTGCTTGTTTTGGTTGTTTACAGGTTTGACATCCAGTTGAACAATGCTATTCTCGCTGAAGATAAGCACAAATCTATGTAAGATTTCGATCACTAACTATCAATGTTTTCAATAATGTTATAGTTAGTTTCAGTGTTTAGTTAACATGCTAATTTAGGATAATCTCAAGTTTGAGTGTTTGCTAAATATGCTGCTAATTTGATTTTATTTTGTGTTAGAATGTCTAATCTCGAGTTTAGCTTATTGATTTTGGTAGTTTTAACGATTGGTAGACATGTTAATTTTATTTCATTTCATTTTGTGCTACAATATATATTTTAGGATAATCTTCAGTTTAGCTTATTTACTTTGGTTATTTGAGTCTTGTTTGCTATCAAGGATTTAATTTTGATGTAATTATTTCTTGGCCATTTTTCTATTTAGGGTGTGTTTGGATGGGGAGTGTTCACTTTTCATTTTTAAATTACGGACACTATGAAATGTTTTTTAAAAAGTTAACATATTAACATGATAAACGATCATATAGTACTTCAATCACATTTAATTTATTTTAGAAAAACATGTTATATAGTCCGTAATTTAAAAAGGAAAAGTAAACTCTCCCCTCCCTTCCCTTCATAAACCCTCAATCCAAACAAACCCTTTGTGTTTTTGTATCTATGAAGCACAAACATAGACACCGGCCACGACACTAACACTGACACTCCGACACCAATATTTTTTTTAGAAAGTGACATAATTCAATGTAATTACAAGTGTCAGTCGGTGTCGGACATGACACGTGTCAGACACCTGGACACACCTTCAATCATAAGTGTCAGTGCAAAAGAGTTTTGTATGCTAAATTCTTTGCGATGGTTTTTACAAGTTTTATGAAAAGACAAGAAATTTTGTGACTTCACAAGATTGCACAATCATTTCAGGTATGATGAAATGACTGCCAAATATAACGTGGAGTACATTGCTGGAGGTAATTTTTGTGTTTTGATTTTTAGATTATTGCGTTTCTGGGAAGGAAAGTAACATTTTTTAATGATGTTTATAAATTTTTTTCTTTTTTGCAGGTGCTACTCAGAATTCAATCAGGGTTGCTCAGGTATTGTAGTTAGTGTGTGATTTCTTTTTTATGAGTTGAGCTGAATCTTGAGTACTTGTGGTAAAAATAGCTTTATCATTTTGTATTTGCTTCTTATAGTGGTTGCTTCAAGTTCCCGGAGCCACTAGTTACATCGGTTGCATTGGGAAGGACAAGTTTGGCGAGGAGATGACCAAGAACTCTAAACAAGCTGGTGTAAATGTAAGTTTAATCTTGTTTCTTTTTCGATCTGCTACACTGTACCAGACCGTAGAATCCACTTCTGAAATTAACTTATTTATTTAACAACTAGGTTCACTATTATGAAGACGAGAATACACCTACAGGAACTTGTGCTGTTTGTGTCGTTGGTGGTGAAAGGTGAGAGGATCAAGCTTTCCTTGTGTTTTAAATGCCCAAAAATCATTCATTTTTTTTACGTCTTTGGCATAGTTTTAAATGCCCAGTTCTTTGAAACCTTCTTTTTGATTGCATGTAGGTCACTTATTGCCAACTTATCAGCTGCAAATTGCTACAAGTCTGAGCATTTGAAGAAACCCGAAAATTGGGCATTAGGTACCTTCTTAATTCAGGATCTTTTTCTATCTTAATTAAATCTGAATCATCAAAATGACTAACATTTGGTTTTGTGCATTTTTTAAATAAATAGTTGAAAAGGCCAAATATTTCTATATTGCTGGATTTTTCCTCACTGTATCCCCCGAATCCATTCAATTAGTTGCTGAACATGCAGCTGCAAATAACAAGGTTAGATATGACTAGAGTTTGTCCTAACCTTCGACTAGAAGTATTTGATACTCAAGTATTTTACTCCCTTGTGCAGGTTTTCATGATGAACCTTTCTGCACCCTTTATCTGTGAGTTCTTTAAGGGTCCACAGGAGAAAGCTCTTCCGTAAGAATCTTTCACCGTCAAATTCAAATAAATGATTTTTTTTCCTTATTGATCTTTGAGAATTTGCATTCTGGGATTCAGATATATGGACTATGTTTTTGGAAATGAGACTGAAGCACGGACATTTTCGAAAGTTCATGGCTGGGAGGTAAATCATTACTTTGCAGATCTGTACCCTTGCAGTTTTGGTTTTACATTTTATTTAATAACTCATCTTGGTTCTTTTCCTATTGACCAGACCGATAATGTCGAGGAGATAGCTATCAAGATATCCCAGTTGCCAAAGGCATCAGGAACACACAAAAGGATTACCGTTATCACACAGGGTGCTGATCCTGTGTGTGTTGCTCAAGATGGGAAAGTGACATTGTACCCTGTAATTCTATTACCTAAAGAGAAACTACTTGATACCAATGGAGCAGGTATTGCCCTATTGCTTTCTGAAGTTGAATATGGAACAAAGTCATACATGTTATGAGCTAGTCATAATATTCTTTTGTTTTGTTTTTGTAGGAGATGCCTTTGTTGGAGGATTTCTTTCACAATTGGTCCAGGAGAAACCTATTGAAGAATGTGTGAGAGCTGGGTGTTACGCAGCAAATGTTATCATTCAAAGGTCCGGCTGCACTTACCCAGAGAAGCCTGATTTTCAATAA
Protein sequence

>MS.gene004151.t1

MALEGVLLGMGNPLLDISAVVDEDFLKKFDIQLNNAILAEDKHKSMYDEMTAKYNVEYIAGGATQNSIRVAQWLLQVPGATSYIGCIGKDKFGEEMTKNSKQAGVNVHYYEDENTPTGTCAVCVVGGERSLIANLSAANCYKSEHLKKPENWALVEKAKYFYIAGFFLTVSPESIQLVAEHAAANNKVFMMNLSAPFICEFFKGPQEKALPYMDYVFGNETEARTFSKVHGWETDNVEEIAIKISQLPKASGTHKRITVITQGADPVCVAQDGKVTLYPVILLPKEKLLDTNGAGDAFVGGFLSQLVQEKPIEECVRAGCYAANVIIQRSGCTYPEKPDFQ