AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032177

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032177.t1 MTR_2g098950 98.480 329 5 0 1 329 1 329 0.0 659
MS.gene032177.t1 MTR_4g067310 89.752 322 33 0 7 328 8 329 0.0 594
MS.gene032177.t1 MTR_1g076850 67.089 316 104 0 14 329 8 323 1.05e-159 449
MS.gene032177.t1 MTR_5g079460 67.492 323 103 2 5 325 57 379 9.93e-153 434
MS.gene032177.t1 MTR_1g101950 63.385 325 113 2 8 326 13 337 1.44e-148 421
MS.gene032177.t1 MTR_6g089480 62.783 309 113 1 21 327 1 309 6.45e-145 411
MS.gene032177.t1 MTR_6g089480 63.004 273 99 1 21 291 1 273 5.90e-127 364
MS.gene032177.t1 MTR_6g089480 63.704 270 96 1 21 288 1 270 9.04e-127 363
MS.gene032177.t1 MTR_7g075860 56.918 318 133 3 14 327 41 358 3.33e-125 363
MS.gene032177.t1 MTR_1g069745 51.020 294 130 4 35 326 4 285 5.26e-100 296
MS.gene032177.t1 MTR_1g057810 48.923 325 155 5 4 325 244 560 2.65e-99 304
MS.gene032177.t1 MTR_1g069490 49.211 317 133 4 12 326 108 398 3.15e-97 293
MS.gene032177.t1 MTR_1g105150 45.846 325 151 6 4 325 244 546 4.35e-87 272
MS.gene032177.t1 MTR_1g054730 45.221 272 120 2 54 325 144 386 2.22e-70 224
MS.gene032177.t1 MTR_4g019165 32.698 367 200 4 7 326 86 452 2.26e-53 182
MS.gene032177.t1 MTR_5g044990 32.686 309 183 5 40 326 250 555 6.59e-50 175
MS.gene032177.t1 MTR_0046s0180 52.000 100 48 0 225 324 8 107 4.66e-31 113
MS.gene032177.t1 MTR_2g072830 27.055 292 181 9 45 327 89 357 4.21e-16 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032177.t1 AT3G59480 82.927 328 52 1 1 328 1 324 0.0 559
MS.gene032177.t1 AT2G31390 83.228 316 53 0 13 328 8 323 0.0 545
MS.gene032177.t1 AT1G06030 79.878 328 63 1 1 328 1 325 0.0 544
MS.gene032177.t1 AT1G06020 79.573 328 63 1 1 328 1 324 0.0 541
MS.gene032177.t1 AT4G10260 68.868 318 99 0 12 329 4 321 1.54e-160 451
MS.gene032177.t1 AT1G66430 64.286 322 113 2 3 323 55 375 2.43e-152 433
MS.gene032177.t1 AT5G51830 65.094 318 109 1 14 329 23 340 1.14e-148 422
MS.gene032177.t1 AT5G51830 65.094 318 109 1 14 329 23 340 1.14e-148 422
MS.gene032177.t1 AT1G50390 61.176 170 37 1 159 328 1 141 2.98e-66 205
MS.gene032177.t1 AT3G54090 33.243 367 197 6 7 326 96 461 7.19e-54 183
MS.gene032177.t1 AT1G69200 31.250 288 176 3 40 306 246 532 1.94e-42 155
MS.gene032177.t1 AT1G17160 27.425 299 175 10 45 328 105 376 2.22e-15 76.6
MS.gene032177.t1 AT1G06730 25.839 298 198 8 46 329 132 420 8.89e-15 75.5

Find 94 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTTTGTCCCAACTGTTTC+CGG 0.182064 2.3:-4508632 MS.gene032177:CDS
ACAAGGAGCAACTTCAAATT+TGG 0.187281 2.3:+4506719 None:intergenic
TTCCTTTGTTGGTGCTTTAT+TGG 0.217574 2.3:-4506474 MS.gene032177:CDS
GAGAAGGCAGCCAGCTTCTT+TGG 0.228047 2.3:+4506913 None:intergenic
AGCAATCACAACTACTAAAA+AGG 0.271618 2.3:-4506200 MS.gene032177:CDS
GCAAGCAAATACTGAGTATC+TGG 0.286211 2.3:-4506838 MS.gene032177:CDS
CTAAGCACTTACTTCTAATA+TGG 0.330358 2.3:+4506430 None:intergenic
AGTTCGAGGTTGAGTTCTTC+AGG 0.330827 2.3:+4508320 None:intergenic
CATGCTCGTCGGGATCTTAA+AGG 0.337842 2.3:-4508477 MS.gene032177:CDS
TGGCTCCACAATCAAGCTTA+TGG 0.338825 2.3:+4506970 None:intergenic
ACTTTGTCCCAACTGTTTCC+GGG 0.348465 2.3:-4508631 MS.gene032177:CDS
GCCATCGCTGTTTCCCGATT+AGG 0.356581 2.3:-4508548 MS.gene032177:CDS
AGGCAGCTTTGCCTCCTAAT+CGG 0.387773 2.3:+4508534 None:intergenic
GGTGCCGGTGATTCCTTTGT+TGG 0.398386 2.3:-4506485 MS.gene032177:CDS
GGCCAAGATTGTTGATGATC+AGG 0.401747 2.3:-4506453 MS.gene032177:CDS
GACGTTCGCTGGTGCACCGC+CGG 0.406668 2.3:+4508571 None:intergenic
CCAAGGTGCACGTACTGCTT+TGG 0.410980 2.3:-4508420 MS.gene032177:CDS
GCCGGGAGCTTTGAGAAAAC+CGG 0.420639 2.3:+4508589 None:intergenic
GTCAATACAGTTGATACAAC+TGG 0.425906 2.3:-4506506 MS.gene032177:CDS
TTGCGAGCTTCTTCCGCTGA+GGG 0.429226 2.3:+4506857 None:intergenic
CACAGTGGTAGGCGGAGGTT+AGG 0.430184 2.3:+4506881 None:intergenic
AAGTTGCTCCTTGTCACTCT+TGG 0.431035 2.3:-4506710 MS.gene032177:CDS
CTTGTCACTCTTGGTGAACA+TGG 0.436126 2.3:-4506701 MS.gene032177:CDS
CAAGGAGCAACTTCAAATTT+GGG 0.437763 2.3:+4506720 None:intergenic
GGCAGCTTTGCCTCCTAATC+GGG 0.442724 2.3:+4508535 None:intergenic
CAGGAATCTATGCTTTGATC+AGG 0.443006 2.3:+4506137 None:intergenic
ATGTAGATCAGCACACTTGA+AGG 0.450810 2.3:-4506948 MS.gene032177:CDS
ACAGCGATGGCGACGTTCGC+TGG 0.452557 2.3:+4508560 None:intergenic
ACGTTCGCTGGTGCACCGCC+GGG 0.457893 2.3:+4508572 None:intergenic
TTCAGTACATCCTCCTCCTT+GGG 0.459048 2.3:+4506160 None:intergenic
GTTCAGTACATCCTCCTCCT+TGG 0.459298 2.3:+4506159 None:intergenic
GCTGCCTTCGTCGGGAAACT+AGG 0.459666 2.3:-4508518 MS.gene032177:CDS
CCGCTGAGGGCCACAGTGGT+AGG 0.460757 2.3:+4506870 None:intergenic
GTCTCCCTTGCAGAAGCCCC+CGG 0.460901 2.3:-4508608 MS.gene032177:CDS
GGCCTGATCATCAACAATCT+TGG 0.466456 2.3:+4506451 None:intergenic
ACTGGTCTCATCGTCAGCTT+CGG 0.467497 2.3:-4508668 MS.gene032177:CDS
TCCTCCTCCTTGGGAAGAGC+AGG 0.469695 2.3:+4506169 None:intergenic
GAGGCAAAGCTGCCTTCGTC+GGG 0.469972 2.3:-4508526 MS.gene032177:CDS
TAGGCGGAGGTTAGGATCAT+AGG 0.483812 2.3:+4506889 None:intergenic
CCGGGAGCTTTGAGAAAACC+GGG 0.487608 2.3:+4508590 None:intergenic
AATGAGCTGGAGTTCCTGAC+TGG 0.488918 2.3:-4506785 MS.gene032177:CDS
TTAAGAAGCATGTCAGCACT+TGG 0.493995 2.3:+4508344 None:intergenic
AGTTCGGTCACATGCTCGTC+GGG 0.494876 2.3:-4508487 MS.gene032177:CDS
CTTGCGAGCTTCTTCCGCTG+AGG 0.497527 2.3:+4506856 None:intergenic
GGAAAAGGCTGATTTGATCA+AGG 0.504055 2.3:-4506816 MS.gene032177:CDS
GGCTTCTGCAAGGGAGACCC+CGG 0.504829 2.3:+4508613 None:intergenic
AATACTGAGTATCTGGGAAA+AGG 0.509401 2.3:-4506831 MS.gene032177:CDS
GGAGGCAAAGCTGCCTTCGT+CGG 0.509809 2.3:-4508527 MS.gene032177:CDS
ATGGAAGTTGCCAAAGAAGC+TGG 0.510846 2.3:-4506923 MS.gene032177:CDS
TGAGACCAGTGCCTGTGGTT+GGG 0.512647 2.3:+4508681 None:intergenic
AAACTAGGCGATGACGAGTT+CGG 0.513562 2.3:-4508503 MS.gene032177:CDS
CATAAACTCACGCTCTCCAT+CGG 0.515761 2.3:+4508379 None:intergenic
ATGCTACTGCTTTGACATTG+TGG 0.517687 2.3:-4506745 MS.gene032177:CDS
GGCATCCCAACCACAGGCAC+TGG 0.519828 2.3:-4508686 MS.gene032177:CDS
GGGAGACCCCGGAAACAGTT+GGG 0.528224 2.3:+4508624 None:intergenic
GTACAGATCTGATGAGTTCG+AGG 0.528295 2.3:+4508306 None:intergenic
GTCGGGATCTTAAAGGAAAA+CGG 0.531500 2.3:-4508470 MS.gene032177:CDS
ACTTCAATGGCTTCAAGCAA+TGG 0.538580 2.3:-4508707 None:intergenic
ATCGCTGTTTCCCGATTAGG+AGG 0.542152 2.3:-4508545 MS.gene032177:CDS
TCCTGCTCTTCCCAAGGAGG+AGG 0.543892 2.3:-4506170 MS.gene032177:CDS
AGGGCCACAGTGGTAGGCGG+AGG 0.545092 2.3:+4506876 None:intergenic
CAAGGTTAGTGATAATGAGC+TGG 0.551346 2.3:-4506798 MS.gene032177:CDS
CAAGCAAATACTGAGTATCT+GGG 0.559518 2.3:-4506837 MS.gene032177:CDS
TTCAAGTGTGCTGATCTACA+TGG 0.561390 2.3:+4506950 None:intergenic
TCAAGCTTATGGATCCATAG+TGG 0.562307 2.3:+4506981 None:intergenic
CTTTGTCCCAACTGTTTCCG+GGG 0.562958 2.3:-4508630 MS.gene032177:CDS
AGGGAGACCCCGGAAACAGT+TGG 0.563390 2.3:+4508623 None:intergenic
AGCACCAACAAAGGAATCAC+CGG 0.564007 2.3:+4506481 None:intergenic
ATCAGCACACTTGAAGGCAA+TGG 0.564340 2.3:-4506942 MS.gene032177:CDS
GGTTAGGATCATAGGAGAGA+AGG 0.565489 2.3:+4506897 None:intergenic
AGCAATTCCTGCTCTTCCCA+AGG 0.570369 2.3:-4506176 MS.gene032177:CDS
TGGATCCATAAGCTTGATTG+TGG 0.577549 2.3:-4506975 MS.gene032177:CDS
GAGTTCGGTCACATGCTCGT+CGG 0.579094 2.3:-4508488 MS.gene032177:CDS
ACAGTTGATACAACTGGTGC+CGG 0.581263 2.3:-4506500 MS.gene032177:CDS
GAAAACGGTGTGGTCGCAGA+AGG 0.582324 2.3:-4508455 MS.gene032177:CDS
TCCTAATCGGGAAACAGCGA+TGG 0.585587 2.3:+4508547 None:intergenic
CCAAAGCAGTACGTGCACCT+TGG 0.592587 2.3:+4508420 None:intergenic
ATGAGACCAGTGCCTGTGGT+TGG 0.592790 2.3:+4508680 None:intergenic
CTCAAGTTTGCAAATGCATG+TGG 0.598828 2.3:-4506223 MS.gene032177:CDS
ATCGCCTAGTTTCCCGACGA+AGG 0.599330 2.3:+4508514 None:intergenic
AGCAATGGCATCCCAACCAC+AGG 0.606388 2.3:-4508692 MS.gene032177:CDS
GGCCAATAAAGCACCAACAA+AGG 0.608161 2.3:+4506472 None:intergenic
TTTGTTACTCTACGCGCCGA+TGG 0.610292 2.3:-4508395 MS.gene032177:CDS
CTGAGGGCCACAGTGGTAGG+CGG 0.610577 2.3:+4506873 None:intergenic
CATGTTCACCAAGAGTGACA+AGG 0.619134 2.3:+4506702 None:intergenic
GGGAGCTTTGAGAAAACCGG+GGG 0.651383 2.3:+4508592 None:intergenic
GACGATGAGACCAGTGCCTG+TGG 0.653048 2.3:+4508676 None:intergenic
CCTACCACTGTGGCCCTCAG+CGG 0.657292 2.3:-4506870 MS.gene032177:CDS
AATTCCTGCTCTTCCCAAGG+AGG 0.668223 2.3:-4506173 MS.gene032177:CDS
CGGGAGCTTTGAGAAAACCG+GGG 0.680003 2.3:+4508591 None:intergenic
TCTTCCGCTGAGGGCCACAG+TGG 0.686880 2.3:+4506866 None:intergenic
GATCTTAAAGGAAAACGGTG+TGG 0.740295 2.3:-4508465 MS.gene032177:CDS
CTAACCTCCGCCTACCACTG+TGG 0.743836 2.3:-4506880 MS.gene032177:CDS
TGGCGCAAGATACTACACCA+AGG 0.783785 2.3:-4506681 MS.gene032177:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAGAAAAATAAAAAGAAT+TGG + chr2.3:4507556-4507575 None:intergenic 15.0%
!! TTACTATATGGTAAATAAAT+TGG - chr2.3:4506973-4506992 MS.gene032177:CDS 15.0%
!!! AAACTTAATTTTAATTTGAC+AGG - chr2.3:4507831-4507850 MS.gene032177:intron 15.0%
!! AAAACCATATCATATAGTAT+CGG + chr2.3:4506788-4506807 None:intergenic 20.0%
!! AAAATTCGCATATATGTAAA+AGG + chr2.3:4507247-4507266 None:intergenic 20.0%
!! AAACATTAAAGAAAATCAAC+TGG + chr2.3:4507431-4507450 None:intergenic 20.0%
!! AAATTCGCATATATGTAAAA+GGG + chr2.3:4507246-4507265 None:intergenic 20.0%
!! AATTCGTCCATAAAATTATA+CGG + chr2.3:4507282-4507301 None:intergenic 20.0%
!! AATTTAAATAATGCAACTTG+TGG - chr2.3:4507575-4507594 MS.gene032177:intron 20.0%
!! AGAATAAAGAAATTACTACA+TGG + chr2.3:4507320-4507339 None:intergenic 20.0%
!! ATCAATTACAAAAACTCAAA+CGG + chr2.3:4508553-4508572 None:intergenic 20.0%
!! CTCAAATTTATTAGTAAGAT+TGG - chr2.3:4507139-4507158 MS.gene032177:intron 20.0%
!! TAGTAATATATAAATTCGCA+TGG - chr2.3:4507620-4507639 MS.gene032177:intron 20.0%
!! TTATTTACCATATAGTAACA+TGG + chr2.3:4506971-4506990 None:intergenic 20.0%
!! TTCTAAACAAAGATATTCTT+TGG + chr2.3:4508251-4508270 None:intergenic 20.0%
!! TTGAATTTGATTATTACACA+TGG + chr2.3:4507702-4507721 None:intergenic 20.0%
!!! ATATGGTTTTGATTTTGTTT+TGG - chr2.3:4506798-4506817 MS.gene032177:CDS 20.0%
!!! TATTCATCCTCTAAATTTTT+AGG - chr2.3:4506861-4506880 MS.gene032177:CDS 20.0%
!!! TGATTTATTTTCATAATTGC+AGG - chr2.3:4508576-4508595 MS.gene032177:CDS 20.0%
!!! TTTTATCTTCATCAACTTTA+AGG + chr2.3:4506892-4506911 None:intergenic 20.0%
!!! TTTTGTATGTTTAGTCTAAT+GGG - chr2.3:4507089-4507108 MS.gene032177:intron 20.0%
!!! TTTTTGTATGTTTAGTCTAA+TGG - chr2.3:4507088-4507107 MS.gene032177:intron 20.0%
! AACCAATAGTACAAAAAGTA+GGG + chr2.3:4508202-4508221 None:intergenic 25.0%
! ATATTAGAAGTAAGTGCTTA+GGG - chr2.3:4508417-4508436 MS.gene032177:CDS 25.0%
! ATTAGTCCTAGATCTAATAA+TGG + chr2.3:4506743-4506762 None:intergenic 25.0%
! CGTAGGAAAAAAAAAGAATT+AGG - chr2.3:4507380-4507399 MS.gene032177:intron 25.0%
! CTTACTAATAAATTTGAGCA+CGG + chr2.3:4507137-4507156 None:intergenic 25.0%
! CTTTAAACCATGTTACTATA+TGG - chr2.3:4506961-4506980 MS.gene032177:CDS 25.0%
! TATATGTAAAAGGGTCAATT+CGG + chr2.3:4507237-4507256 None:intergenic 25.0%
! TCAATTACAAAAACTCAAAC+GGG + chr2.3:4508552-4508571 None:intergenic 25.0%
!! CATTGACTAAGTACATTATT+TGG - chr2.3:4507663-4507682 MS.gene032177:intron 25.0%
!! TGATTTACCATTTGAGTTAT+GGG - chr2.3:4507793-4507812 MS.gene032177:intron 25.0%
!! TTTTGATCCAATTGTAAGAT+CGG - chr2.3:4507168-4507187 MS.gene032177:intron 25.0%
!!! AAGTTGATGAAGATAAAAAG+AGG - chr2.3:4506894-4506913 MS.gene032177:CDS 25.0%
!!! TCTTTTTTTTTCCTACGTAA+TGG + chr2.3:4507377-4507396 None:intergenic 25.0%
ACCTCAAGTTTGAATAATGT+TGG + chr2.3:4507067-4507086 None:intergenic 30.0%
ACGTAATGGTAACTATCAAA+TGG + chr2.3:4507363-4507382 None:intergenic 30.0%
ACTTAGTCAATGATGTAACA+CGG + chr2.3:4507655-4507674 None:intergenic 30.0%
AGCAATCACAACTACTAAAA+AGG - chr2.3:4508645-4508664 MS.gene032177:CDS 30.0%
AGTAGCTAATGTGTATTAAG+GGG + chr2.3:4508447-4508466 None:intergenic 30.0%
ATACACATTAGCTACTCAAA+TGG - chr2.3:4508451-4508470 MS.gene032177:CDS 30.0%
ATTTAGCATGACGACAAAAT+TGG + chr2.3:4506937-4506956 None:intergenic 30.0%
CAACCAATAGTACAAAAAGT+AGG + chr2.3:4508203-4508222 None:intergenic 30.0%
CAATTACAAAAACTCAAACG+GGG + chr2.3:4508551-4508570 None:intergenic 30.0%
CATATTAGAAGTAAGTGCTT+AGG - chr2.3:4508416-4508435 MS.gene032177:CDS 30.0%
CATTGATAAAATTACTGCCT+TGG - chr2.3:4507760-4507779 MS.gene032177:intron 30.0%
CTAAGCACTTACTTCTAATA+TGG + chr2.3:4508418-4508437 None:intergenic 30.0%
CTAATAAGCTGCATAATTTG+AGG + chr2.3:4507000-4507019 None:intergenic 30.0%
GAGTAGCTAATGTGTATTAA+GGG + chr2.3:4508448-4508467 None:intergenic 30.0%
GCAATCACAACTACTAAAAA+GGG - chr2.3:4508646-4508665 MS.gene032177:CDS 30.0%
TAAATCACACAATTCAACCA+AGG + chr2.3:4507780-4507799 None:intergenic 30.0%
TCCAACATTATTCAAACTTG+AGG - chr2.3:4507063-4507082 MS.gene032177:intron 30.0%
TGAGTAGCTAATGTGTATTA+AGG + chr2.3:4508449-4508468 None:intergenic 30.0%
! GATTTACCATTTGAGTTATG+GGG - chr2.3:4507794-4507813 MS.gene032177:intron 30.0%
! GTGATTTACCATTTGAGTTA+TGG - chr2.3:4507792-4507811 MS.gene032177:intron 30.0%
! TAAAAAGAGGCTTTTGAATG+AGG - chr2.3:4506907-4506926 MS.gene032177:CDS 30.0%
! TGTTGGTCCGTATAATTTTA+TGG - chr2.3:4507272-4507291 MS.gene032177:intron 30.0%
! TTTGATAGTTACCATTACGT+AGG - chr2.3:4507363-4507382 MS.gene032177:intron 30.0%
!! CACAAATCTGTTTCTATCAT+TGG + chr2.3:4507199-4507218 None:intergenic 30.0%
!! TATGCGAATTTTAGAGATGT+TGG - chr2.3:4507255-4507274 MS.gene032177:intron 30.0%
!!! GGTTTTGATTTTGTTTTGGT+GGG - chr2.3:4506802-4506821 MS.gene032177:CDS 30.0%
!!! TGGTTTTGATTTTGTTTTGG+TGG - chr2.3:4506801-4506820 MS.gene032177:CDS 30.0%
AATACTGAGTATCTGGGAAA+AGG - chr2.3:4508014-4508033 MS.gene032177:intron 35.0%
ACAAGGAGCAACTTCAAATT+TGG + chr2.3:4508129-4508148 None:intergenic 35.0%
AGAAACAGATTTGTGCATGT+TGG - chr2.3:4507204-4507223 MS.gene032177:intron 35.0%
AGCAATTATATTTCATGGCG+TGG - chr2.3:4507527-4507546 MS.gene032177:intron 35.0%
CAAGCAAATACTGAGTATCT+GGG - chr2.3:4508008-4508027 MS.gene032177:intron 35.0%
CAAGGAGCAACTTCAAATTT+GGG + chr2.3:4508128-4508147 None:intergenic 35.0%
CATGAAAATTCTGGCAAAAG+GGG + chr2.3:4508299-4508318 None:intergenic 35.0%
CCATGAAAATTCTGGCAAAA+GGG + chr2.3:4508300-4508319 None:intergenic 35.0%
CTACTTGTCCATGAAAATTC+TGG + chr2.3:4508308-4508327 None:intergenic 35.0%
GACTTCAAATTTGTGTGTTG+AGG - chr2.3:4506668-4506687 MS.gene032177:intron 35.0%
GCAAATTAGTCCATATCACT+TGG - chr2.3:4507019-4507038 MS.gene032177:intron 35.0%
GCTACCGATACTATATGATA+TGG - chr2.3:4506781-4506800 MS.gene032177:CDS 35.0%
GCTATTATTGTTGGATTCTG+AGG + chr2.3:4507454-4507473 None:intergenic 35.0%
GCTGGAGCAATTATATTTCA+TGG - chr2.3:4507522-4507541 MS.gene032177:intron 35.0%
GGTGACTCCTAAAAATTTAG+AGG + chr2.3:4506871-4506890 None:intergenic 35.0%
TCCATGAAAATTCTGGCAAA+AGG + chr2.3:4508301-4508320 None:intergenic 35.0%
TCGTCGTATGCTATTATTGT+TGG + chr2.3:4507463-4507482 None:intergenic 35.0%
! GTCAATACAGTTGATACAAC+TGG - chr2.3:4508339-4508358 MS.gene032177:CDS 35.0%
! TAGGGACATAGATTTTACCT+TGG + chr2.3:4508184-4508203 None:intergenic 35.0%
! TTCCTTTGTTGGTGCTTTAT+TGG - chr2.3:4508371-4508390 MS.gene032177:CDS 35.0%
!! GAAGGTATAACTTTTGACCA+AGG - chr2.3:4506408-4506427 MS.gene032177:intron 35.0%
!!! GGATAATCTTTTGACTTGCA+AGG + chr2.3:4507046-4507065 None:intergenic 35.0%
!!! GTCCCTACTTTTTGTACTAT+TGG - chr2.3:4508197-4508216 MS.gene032177:intron 35.0%
!!! TTTGATTTTGTTTTGGTGGG+TGG - chr2.3:4506805-4506824 MS.gene032177:CDS 35.0%
ATGCTACTGCTTTGACATTG+TGG - chr2.3:4508100-4508119 MS.gene032177:intron 40.0%
ATGTAAAAGGGTCAATTCGG+TGG + chr2.3:4507234-4507253 None:intergenic 40.0%
ATGTAGATCAGCACACTTGA+AGG - chr2.3:4507897-4507916 MS.gene032177:intron 40.0%
CTCAAGTTTGCAAATGCATG+TGG - chr2.3:4508622-4508641 MS.gene032177:CDS 40.0%
GATCCAATTGTAAGATCGGT+TGG - chr2.3:4507172-4507191 MS.gene032177:intron 40.0%
GATCTTAAAGGAAAACGGTG+TGG - chr2.3:4506380-4506399 MS.gene032177:intron 40.0%
GCAAGCAAATACTGAGTATC+TGG - chr2.3:4508007-4508026 MS.gene032177:intron 40.0%
GTCGGGATCTTAAAGGAAAA+CGG - chr2.3:4506375-4506394 MS.gene032177:intron 40.0%
TCAAGCTTATGGATCCATAG+TGG + chr2.3:4507867-4507886 None:intergenic 40.0%
TGGATCCATAAGCTTGATTG+TGG - chr2.3:4507870-4507889 MS.gene032177:intron 40.0%
TGGGGTCCATTATTAGATCT+AGG - chr2.3:4506734-4506753 MS.gene032177:CDS 40.0%
! CAAGGTTAGTGATAATGAGC+TGG - chr2.3:4508047-4508066 MS.gene032177:intron 40.0%
! CAGGCAAAAGTTTTCCACTA+TGG - chr2.3:4507850-4507869 MS.gene032177:intron 40.0%
! CCCTTTTGCCAGAATTTTCA+TGG - chr2.3:4508297-4508316 MS.gene032177:intron 40.0%
! TACCATTTGAGTTATGGGGT+AGG - chr2.3:4507798-4507817 MS.gene032177:intron 40.0%
! TTAAGAAGCATGTCAGCACT+TGG + chr2.3:4506504-4506523 None:intergenic 40.0%
! TTCAAGTGTGCTGATCTACA+TGG + chr2.3:4507898-4507917 None:intergenic 40.0%
!! CAATTTTGTCACTTCCAGTC+AGG + chr2.3:4508077-4508096 None:intergenic 40.0%
!! CGTTGGTAGGTTTTATCAGT+GGG - chr2.3:4507486-4507505 MS.gene032177:intron 40.0%
!! GGAAAAGGCTGATTTGATCA+AGG - chr2.3:4508029-4508048 MS.gene032177:intron 40.0%
!! GTTGGTAGGTTTTATCAGTG+GGG - chr2.3:4507487-4507506 MS.gene032177:intron 40.0%
!! TCATATAGTATCGGTAGCTC+TGG + chr2.3:4506779-4506798 None:intergenic 40.0%
!! TTGGTAGGTTTTATCAGTGG+GGG - chr2.3:4507488-4507507 MS.gene032177:intron 40.0%
AAAATTCTGGCAAAAGGGGC+AGG + chr2.3:4508295-4508314 None:intergenic 45.0%
AAACTAGGCGATGACGAGTT+CGG - chr2.3:4506342-4506361 MS.gene032177:intron 45.0%
AAGTTGCTCCTTGTCACTCT+TGG - chr2.3:4508135-4508154 MS.gene032177:intron 45.0%
ACTTTGTCCCAACTGTTTCC+GGG - chr2.3:4506214-4506233 MS.gene032177:CDS 45.0%
AGCACCAACAAAGGAATCAC+CGG + chr2.3:4508367-4508386 None:intergenic 45.0%
AGTTCGAGGTTGAGTTCTTC+AGG + chr2.3:4506528-4506547 None:intergenic 45.0%
ATCAGCACACTTGAAGGCAA+TGG - chr2.3:4507903-4507922 MS.gene032177:intron 45.0%
ATGGAAGTTGCCAAAGAAGC+TGG - chr2.3:4507922-4507941 MS.gene032177:intron 45.0%
CACCTACCCCATAACTCAAA+TGG + chr2.3:4507803-4507822 None:intergenic 45.0%
CATAAACTCACGCTCTCCAT+CGG + chr2.3:4506469-4506488 None:intergenic 45.0%
CATGTTCACCAAGAGTGACA+AGG + chr2.3:4508146-4508165 None:intergenic 45.0%
CTTGCAAGGACCAAGTGATA+TGG + chr2.3:4507032-4507051 None:intergenic 45.0%
CTTGTCACTCTTGGTGAACA+TGG - chr2.3:4508144-4508163 MS.gene032177:intron 45.0%
GACTTTGTCCCAACTGTTTC+CGG - chr2.3:4506213-4506232 MS.gene032177:CDS 45.0%
GGACCAACCGATCTTACAAT+TGG + chr2.3:4507178-4507197 None:intergenic 45.0%
GGCCTGATCATCAACAATCT+TGG + chr2.3:4508397-4508416 None:intergenic 45.0%
GGTTAGGATCATAGGAGAGA+AGG + chr2.3:4507951-4507970 None:intergenic 45.0%
GTACAGATCTGATGAGTTCG+AGG + chr2.3:4506542-4506561 None:intergenic 45.0%
TGGCTCCACAATCAAGCTTA+TGG + chr2.3:4507878-4507897 None:intergenic 45.0%
TTCAGTACATCCTCCTCCTT+GGG + chr2.3:4508688-4508707 None:intergenic 45.0%
! ACAGTTGATACAACTGGTGC+CGG - chr2.3:4508345-4508364 MS.gene032177:CDS 45.0%
! ATAGCATACGACGAAAGCGT+TGG - chr2.3:4507469-4507488 MS.gene032177:intron 45.0%
! GGCCAATAAAGCACCAACAA+AGG + chr2.3:4508376-4508395 None:intergenic 45.0%
!! GCGTTGGTAGGTTTTATCAG+TGG - chr2.3:4507485-4507504 MS.gene032177:intron 45.0%
!! GGCCAAGATTGTTGATGATC+AGG - chr2.3:4508392-4508411 MS.gene032177:CDS 45.0%
!! TGGTAGGTTTTATCAGTGGG+GGG - chr2.3:4507489-4507508 MS.gene032177:intron 45.0%
!!! GATTTTGTTTTGGTGGGTGG+AGG - chr2.3:4506808-4506827 MS.gene032177:CDS 45.0%
AATGAGCTGGAGTTCCTGAC+TGG - chr2.3:4508060-4508079 MS.gene032177:intron 50.0%
AATTCCTGCTCTTCCCAAGG+AGG - chr2.3:4508672-4508691 MS.gene032177:CDS 50.0%
AGCAATTCCTGCTCTTCCCA+AGG - chr2.3:4508669-4508688 MS.gene032177:CDS 50.0%
AGGCAGCTTTGCCTCCTAAT+CGG + chr2.3:4506314-4506333 None:intergenic 50.0%
CATGCTCGTCGGGATCTTAA+AGG - chr2.3:4506368-4506387 MS.gene032177:intron 50.0%
CGACATTGACATGTCAGCGT+TGG - chr2.3:4506695-4506714 MS.gene032177:CDS 50.0%
CTTTGTCCCAACTGTTTCCG+GGG - chr2.3:4506215-4506234 MS.gene032177:CDS 50.0%
GTTCAGTACATCCTCCTCCT+TGG + chr2.3:4508689-4508708 None:intergenic 50.0%
TAGGCGGAGGTTAGGATCAT+AGG + chr2.3:4507959-4507978 None:intergenic 50.0%
TCCTAATCGGGAAACAGCGA+TGG + chr2.3:4506301-4506320 None:intergenic 50.0%
TGGCGCAAGATACTACACCA+AGG - chr2.3:4508164-4508183 MS.gene032177:intron 50.0%
TGGTGTGTTCATGTGGTGGT+GGG - chr2.3:4506715-4506734 MS.gene032177:CDS 50.0%
TTGGTGTGTTCATGTGGTGG+TGG - chr2.3:4506714-4506733 MS.gene032177:CDS 50.0%
TTTGTTACTCTACGCGCCGA+TGG - chr2.3:4506450-4506469 MS.gene032177:CDS 50.0%
! ACTGGTCTCATCGTCAGCTT+CGG - chr2.3:4506177-4506196 MS.gene032177:CDS 50.0%
! ATCGCTGTTTCCCGATTAGG+AGG - chr2.3:4506300-4506319 MS.gene032177:intron 50.0%
!! AGGTTTTATCAGTGGGGGGT+TGG - chr2.3:4507493-4507512 MS.gene032177:intron 50.0%
!! CATACGACGAAAGCGTTGGT+AGG - chr2.3:4507473-4507492 MS.gene032177:intron 50.0%
!! GGTTTTATCAGTGGGGGGTT+GGG - chr2.3:4507494-4507513 MS.gene032177:intron 50.0%
!! TCAGCGTTGGTGTGTTCATG+TGG - chr2.3:4506708-4506727 MS.gene032177:CDS 50.0%
!! TTTTGGTGGGTGGAGGTTGT+TGG - chr2.3:4506815-4506834 MS.gene032177:CDS 50.0%
AAAACCGGGGGCTTCTGCAA+GGG + chr2.3:4506244-4506263 None:intergenic 55.0%
AGCAATGGCATCCCAACCAC+AGG - chr2.3:4506153-4506172 MS.gene032177:CDS 55.0%
AGTTCGGTCACATGCTCGTC+GGG - chr2.3:4506358-4506377 MS.gene032177:intron 55.0%
ATCGCCTAGTTTCCCGACGA+AGG + chr2.3:4506334-4506353 None:intergenic 55.0%
ATGAGACCAGTGCCTGTGGT+TGG + chr2.3:4506168-4506187 None:intergenic 55.0%
CCAAAGCAGTACGTGCACCT+TGG + chr2.3:4506428-4506447 None:intergenic 55.0%
CCAAGGTGCACGTACTGCTT+TGG - chr2.3:4506425-4506444 MS.gene032177:intron 55.0%
GAAAACGGTGTGGTCGCAGA+AGG - chr2.3:4506390-4506409 MS.gene032177:intron 55.0%
GAGAAGGCAGCCAGCTTCTT+TGG + chr2.3:4507935-4507954 None:intergenic 55.0%
GAGTTCGGTCACATGCTCGT+CGG - chr2.3:4506357-4506376 MS.gene032177:intron 55.0%
GGCAGCTTTGCCTCCTAATC+GGG + chr2.3:4506313-4506332 None:intergenic 55.0%
TGAGACCAGTGCCTGTGGTT+GGG + chr2.3:4506167-4506186 None:intergenic 55.0%
TTGCGAGCTTCTTCCGCTGA+GGG + chr2.3:4507991-4508010 None:intergenic 55.0%
! CCCGGTTTTCTCAAAGCTCC+CGG - chr2.3:4506255-4506274 MS.gene032177:intron 55.0%
! GCCATCGCTGTTTCCCGATT+AGG - chr2.3:4506297-4506316 MS.gene032177:intron 55.0%
! GCGTTGGTGTGTTCATGTGG+TGG - chr2.3:4506711-4506730 MS.gene032177:CDS 55.0%
! GGTGTGTTCATGTGGTGGTG+GGG - chr2.3:4506716-4506735 MS.gene032177:CDS 55.0%
! GGTTTTCTCAAAGCTCCCGG+CGG - chr2.3:4506258-4506277 MS.gene032177:intron 55.0%
!! CCGGGAGCTTTGAGAAAACC+GGG + chr2.3:4506258-4506277 None:intergenic 55.0%
!! CGGGAGCTTTGAGAAAACCG+GGG + chr2.3:4506257-4506276 None:intergenic 55.0%
!! GCCGGGAGCTTTGAGAAAAC+CGG + chr2.3:4506259-4506278 None:intergenic 55.0%
!! GGGAGCTTTGAGAAAACCGG+GGG + chr2.3:4506256-4506275 None:intergenic 55.0%
!! GGTGCCGGTGATTCCTTTGT+TGG - chr2.3:4508360-4508379 MS.gene032177:CDS 55.0%
AGGGAGACCCCGGAAACAGT+TGG + chr2.3:4506225-4506244 None:intergenic 60.0%
CACAGTGGTAGGCGGAGGTT+AGG + chr2.3:4507967-4507986 None:intergenic 60.0%
CTAACCTCCGCCTACCACTG+TGG - chr2.3:4507965-4507984 MS.gene032177:intron 60.0%
CTTGCGAGCTTCTTCCGCTG+AGG + chr2.3:4507992-4508011 None:intergenic 60.0%
GAAAACCGGGGGCTTCTGCA+AGG + chr2.3:4506245-4506264 None:intergenic 60.0%
GACGATGAGACCAGTGCCTG+TGG + chr2.3:4506172-4506191 None:intergenic 60.0%
GAGGCAAAGCTGCCTTCGTC+GGG - chr2.3:4506319-4506338 MS.gene032177:intron 60.0%
GCTGCCTTCGTCGGGAAACT+AGG - chr2.3:4506327-4506346 MS.gene032177:intron 60.0%
GGAGGCAAAGCTGCCTTCGT+CGG - chr2.3:4506318-4506337 MS.gene032177:intron 60.0%
GGGAGACCCCGGAAACAGTT+GGG + chr2.3:4506224-4506243 None:intergenic 60.0%
GGTGGGTGGAGGTTGTTGGT+TGG - chr2.3:4506819-4506838 MS.gene032177:CDS 60.0%
TCCTCCTCCTTGGGAAGAGC+AGG + chr2.3:4508679-4508698 None:intergenic 60.0%
TCCTGCTCTTCCCAAGGAGG+AGG - chr2.3:4508675-4508694 MS.gene032177:CDS 60.0%
ACAGCGATGGCGACGTTCGC+TGG + chr2.3:4506288-4506307 None:intergenic 65.0%
CCTACCACTGTGGCCCTCAG+CGG - chr2.3:4507975-4507994 MS.gene032177:intron 65.0%
CTGAGGGCCACAGTGGTAGG+CGG + chr2.3:4507975-4507994 None:intergenic 65.0%
GGCATCCCAACCACAGGCAC+TGG - chr2.3:4506159-4506178 MS.gene032177:CDS 65.0%
GGCTTCTGCAAGGGAGACCC+CGG + chr2.3:4506235-4506254 None:intergenic 65.0%
GTCTCCCTTGCAGAAGCCCC+CGG - chr2.3:4506237-4506256 MS.gene032177:CDS 65.0%
GTGGGGGGTTGGGATTTCGC+TGG - chr2.3:4507504-4507523 MS.gene032177:intron 65.0%
TCTTCCGCTGAGGGCCACAG+TGG + chr2.3:4507982-4508001 None:intergenic 65.0%
ACGTTCGCTGGTGCACCGCC+GGG + chr2.3:4506276-4506295 None:intergenic 70.0%
AGGGCCACAGTGGTAGGCGG+AGG + chr2.3:4507972-4507991 None:intergenic 70.0%
CCGCTGAGGGCCACAGTGGT+AGG + chr2.3:4507978-4507997 None:intergenic 70.0%
GACGTTCGCTGGTGCACCGC+CGG + chr2.3:4506277-4506296 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 4506144 4508723 4506144 ID=MS.gene032177
chr2.3 mRNA 4506144 4508723 4506144 ID=MS.gene032177.t1;Parent=MS.gene032177
chr2.3 exon 4508310 4508723 4508310 ID=MS.gene032177.t1.exon1;Parent=MS.gene032177.t1
chr2.3 CDS 4508310 4508723 4508310 ID=cds.MS.gene032177.t1;Parent=MS.gene032177.t1
chr2.3 exon 4506682 4507014 4506682 ID=MS.gene032177.t1.exon2;Parent=MS.gene032177.t1
chr2.3 CDS 4506682 4507014 4506682 ID=cds.MS.gene032177.t1;Parent=MS.gene032177.t1
chr2.3 exon 4506442 4506558 4506442 ID=MS.gene032177.t1.exon3;Parent=MS.gene032177.t1
chr2.3 CDS 4506442 4506558 4506442 ID=cds.MS.gene032177.t1;Parent=MS.gene032177.t1
chr2.3 exon 4506144 4506269 4506144 ID=MS.gene032177.t1.exon4;Parent=MS.gene032177.t1
chr2.3 CDS 4506144 4506269 4506144 ID=cds.MS.gene032177.t1;Parent=MS.gene032177.t1
Gene Sequence

>MS.gene032177

ATGGCTTCAAGCAATGGCATCCCAACCACAGGCACTGGTCTCATCGTCAGCTTCGGTGAGATGCTGATCGACTTTGTCCCAACTGTTTCCGGGGTCTCCCTTGCAGAAGCCCCCGGTTTTCTCAAAGCTCCCGGCGGTGCACCAGCGAACGTCGCCATCGCTGTTTCCCGATTAGGAGGCAAAGCTGCCTTCGTCGGGAAACTAGGCGATGACGAGTTCGGTCACATGCTCGTCGGGATCTTAAAGGAAAACGGTGTGGTCGCAGAAGGTATAACTTTTGACCAAGGTGCACGTACTGCTTTGGCGTTTGTTACTCTACGCGCCGATGGAGAGCGTGAGTTTATGTTTTATAGAAATCCAAGTGCTGACATGCTTCTTAAACCTGAAGAACTCAACCTCGAACTCATCAGATCTGTACGTAACATAACAACTTTATCTATCTCTTTTGTTTTTTGTTATGTTTTGTTTTTCTGTAGTACTTTGTTGTTTCGATGTTATTTTCGTATCTGTGTCTTGTTTGTAACGACTTCAAATTTGTGTGTTGAGGTAAACGACATTGACATGTCAGCGTTGGTGTGTTCATGTGGTGGTGGGGTCCATTATTAGATCTAGGACTAATCTTACGTTACGCACCAGAGCTACCGATACTATATGATATGGTTTTGATTTTGTTTTGGTGGGTGGAGGTTGTTGGTTGGTGTTATTGTTACTATGTAGTATTCATCCTCTAAATTTTTAGGAGTCACCTTAAAGTTGATGAAGATAAAAAGAGGCTTTTGAATGAGGTTGACCAATTTTGTCGTCATGCTAAATTAATCTTTAAACCATGTTACTATATGGTAAATAAATTGGTCCTCAAATTATGCAGCTTATTAGCAAATTAGTCCATATCACTTGGTCCTTGCAAGTCAAAAGATTATCCAACATTATTCAAACTTGAGGTCTTTTTGTATGTTTAGTCTAATGGGCGCACAGATTTTGAGTCTTGTACCGTGCTCAAATTTATTAGTAAGATTGGTTGAACTTTTGATCCAATTGTAAGATCGGTTGGTCCAATGATAGAAACAGATTTGTGCATGTTGGTGCACCACCGAATTGACCCTTTTACATATATGCGAATTTTAGAGATGTTGGTCCGTATAATTTTATGGACGAATTGATAATTTACTCTTACCATGTAGTAATTTCTTTATTCTCACTACACGTGATTCACGTGCCATTTGATAGTTACCATTACGTAGGAAAAAAAAAGAATTAGGATGATGATGAATCATTATTTTTGATCCAGTTGATTTTCTTTAATGTTTCCTCAGAATCCAACAATAATAGCATACGACGAAAGCGTTGGTAGGTTTTATCAGTGGGGGGTTGGGATTTCGCTGGAGCAATTATATTTCATGGCGTGGTGTCCAATTCTTTTTATTTTTCTAGAATTTAAATAATGCAACTTGTGGTTATTCAAACATATATAAAAATTAGTAATATATAAATTCGCATGGTGTTTCACGCCGTGTTACATCATTGACTAAGTACATTATTTGGCAGCTTATTCATGCCATGTGTAATAATCAAATTCAAATTCGTATAGTGAACTAAAAATTTGTTAAAAAATTCATCATTGATAAAATTACTGCCTTGGTTGAATTGTGTGATTTACCATTTGAGTTATGGGGTAGGTGACTAATTGAAACTTAATTTTAATTTGACAGGCAAAAGTTTTCCACTATGGATCCATAAGCTTGATTGTGGAGCCATGTAGATCAGCACACTTGAAGGCAATGGAAGTTGCCAAAGAAGCTGGCTGCCTTCTCTCCTATGATCCTAACCTCCGCCTACCACTGTGGCCCTCAGCGGAAGAAGCTCGCAAGCAAATACTGAGTATCTGGGAAAAGGCTGATTTGATCAAGGTTAGTGATAATGAGCTGGAGTTCCTGACTGGAAGTGACAAAATTGATGATGCTACTGCTTTGACATTGTGGCACCCAAATTTGAAGTTGCTCCTTGTCACTCTTGGTGAACATGGCGCAAGATACTACACCAAGGTAAAATCTATGTCCCTACTTTTTGTACTATTGGTTGTTAAACTTTGTCTAAAAACTGTAAACCAAAGAATATCTTTGTTTAGAAACACTAATGAGTTTTTTTTTGCCTGCCCCTTTTGCCAGAATTTTCATGGACAAGTAGATGCATTCAAAGTCAATACAGTTGATACAACTGGTGCCGGTGATTCCTTTGTTGGTGCTTTATTGGCCAAGATTGTTGATGATCAGGCCATATTAGAAGTAAGTGCTTAGGGCTAACCCCTTAATACACATTAGCTACTCAAATGGAAATACACAAGTTTAACTGATTTTGTTTTTAATTTTCTAATATTATCTAAATTTCTGAGTAACGCACTATAAAGCCCCGTTTGAGTTTTTGTAATTGATGATTGATTTATTTTCATAATTGCAGGATGAATCAAAGTTAAGAGAAGTACTCAAGTTTGCAAATGCATGTGGAGCAATCACAACTACTAAAAAGGGAGCAATTCCTGCTCTTCCCAAGGAGGAGGATGTACTGAACCTGATCAAAGCATAG
Protein sequence

>MS.gene032177.t1

MASSNGIPTTGTGLIVSFGEMLIDFVPTVSGVSLAEAPGFLKAPGGAPANVAIAVSRLGGKAAFVGKLGDDEFGHMLVGILKENGVVAEGITFDQGARTALAFVTLRADGEREFMFYRNPSADMLLKPEELNLELIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGCLLSYDPNLRLPLWPSAEEARKQILSIWEKADLIKVSDNELEFLTGSDKIDDATALTLWHPNLKLLLVTLGEHGARYYTKNFHGQVDAFKVNTVDTTGAGDSFVGALLAKIVDDQAILEDESKLREVLKFANACGAITTTKKGAIPALPKEEDVLNLIKA