AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004219

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004219.t1 MTR_2g015500 86.008 243 34 0 1 243 1 243 1.51e-160 444
MS.gene004219.t1 MTR_2g015510 82.231 242 41 1 2 243 3 242 5.07e-151 421
MS.gene004219.t1 MTR_2g015480 73.478 230 42 5 12 239 65 277 8.59e-110 318
MS.gene004219.t1 MTR_4g105680 45.249 221 112 3 25 242 51 265 7.38e-58 185
MS.gene004219.t1 MTR_7g068530 37.736 212 122 2 4 215 6 207 2.31e-50 165
MS.gene004219.t1 MTR_4g016510 37.264 212 125 3 4 215 6 209 1.80e-49 163
MS.gene004219.t1 MTR_7g068550 38.208 212 121 3 4 215 6 207 2.05e-49 163
MS.gene004219.t1 MTR_7g068460 36.449 214 126 3 2 215 31 234 1.67e-46 156
MS.gene004219.t1 MTR_3g070460 35.981 214 125 3 4 215 6 209 7.92e-45 151
MS.gene004219.t1 MTR_7g068500 36.279 215 125 4 2 215 30 233 2.67e-44 150
MS.gene004219.t1 MTR_7g068470 33.962 212 130 4 4 215 2 203 4.69e-41 141
MS.gene004219.t1 MTR_7g068470 34.434 212 125 5 4 215 2 199 1.21e-40 140
MS.gene004219.t1 MTR_7g068450 32.083 240 153 3 4 239 2 235 9.56e-39 135
MS.gene004219.t1 MTR_4g016500 30.137 146 64 3 33 178 277 384 6.51e-17 79.7
MS.gene004219.t1 MTR_7g068520 47.458 59 30 1 4 62 3 60 6.35e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004219.t1 AT5G45920 68.050 241 70 2 4 242 3 238 5.58e-121 344
MS.gene004219.t1 AT5G45920 68.050 241 70 2 4 242 3 238 5.58e-121 344
MS.gene004219.t1 AT5G45920 70.968 186 47 2 4 187 3 183 1.67e-95 278
MS.gene004219.t1 AT5G45920 70.968 186 47 2 4 187 3 183 1.67e-95 278
MS.gene004219.t1 AT5G62930 52.066 242 107 2 4 242 2 237 4.45e-82 246
MS.gene004219.t1 AT5G62930 52.066 242 107 2 4 242 28 263 1.76e-81 245
MS.gene004219.t1 AT5G62930 49.438 178 87 1 68 242 2 179 3.51e-53 170
MS.gene004219.t1 AT2G38180 38.389 211 120 3 4 214 6 206 8.63e-46 155
MS.gene004219.t1 AT3G11210 35.047 214 127 3 4 215 6 209 1.22e-44 150

Find 64 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTCATTTGTCCTTTC+AGG 0.154934 2.4:-69331701 None:intergenic
TTGGATCAATATCAGCTAAA+AGG 0.242460 2.4:-69332196 None:intergenic
AAATTCCTTTCATTGATCTT+TGG 0.243390 2.4:+69331768 MS.gene004219:CDS
AAGTCGTGCTTCTTCATCTA+TGG 0.253796 2.4:-69331318 None:intergenic
AATGCCTTCAGTGGATCATT+TGG 0.343903 2.4:-69332215 None:intergenic
TCCTTTGGTGTTGGAGGATG+GGG 0.361517 2.4:+69328968 MS.gene004219:CDS
TCGTCACCGCCATGTGATAC+TGG 0.369729 2.4:-69330465 None:intergenic
CAAAGCAACAGGAGCAGTTT+CGG 0.379507 2.4:-69330493 None:intergenic
GAAAGGAATTTGACACTCAT+TGG 0.387705 2.4:-69331756 None:intergenic
CTATGGGAGGAGGAGTTATA+AGG 0.399420 2.4:-69331301 None:intergenic
TGGATCAATATCAGCTAAAA+GGG 0.400368 2.4:-69332195 None:intergenic
GACGGTTTGCATCTCACTAA+TGG 0.400983 2.4:+69332089 MS.gene004219:CDS
TCGATCACTGAAGAATCCTT+TGG 0.438191 2.4:+69328953 MS.gene004219:CDS
AGGGAATCAATTTGTGTTTG+AGG 0.442405 2.4:+69332112 MS.gene004219:CDS
ACTAAAATGCAACGGTTCCC+TGG 0.444415 2.4:+69331791 MS.gene004219:CDS
AAGAAAACTGTCAAAGCAAC+AGG 0.453323 2.4:-69330504 None:intergenic
AATCCTTTGGTGTTGGAGGA+TGG 0.456779 2.4:+69328966 MS.gene004219:CDS
ATCCTTTGGTGTTGGAGGAT+GGG 0.456978 2.4:+69328967 MS.gene004219:CDS
GTCCTTTCAGGAAGCCCCTC+CGG 0.465657 2.4:-69331689 None:intergenic
AAATGCAACGGTTCCCTGGA+TGG 0.484613 2.4:+69331795 MS.gene004219:CDS
GGCACTGAAGGTGTTGGAAA+GGG 0.491788 2.4:+69330437 MS.gene004219:CDS
TATAACAAAACATGACTAAG+AGG 0.493140 2.4:+69328906 None:intergenic
ACTGAAGAATCCTTTGGTGT+TGG 0.507462 2.4:+69328959 MS.gene004219:CDS
TAGATGAAGAAGCACGACTT+AGG 0.509915 2.4:+69331321 MS.gene004219:CDS
GAAGAATCCTTTGGTGTTGG+AGG 0.526860 2.4:+69328962 MS.gene004219:CDS
TGCCAACTATTTCTCTCGTA+CGG 0.529977 2.4:+69329000 MS.gene004219:CDS
ATCCGGAGGGGCTTCCTGAA+AGG 0.532035 2.4:+69331687 MS.gene004219:CDS
TTATTTGTTTGTGAACAATC+CGG 0.532846 2.4:+69331670 MS.gene004219:CDS
TCACCGAAAAGATATATTTG+TGG 0.535472 2.4:-69328929 None:intergenic
AGTCGTGCTTCTTCATCTAT+GGG 0.542373 2.4:-69331317 None:intergenic
AATGCAACGGTTCCCTGGAT+GGG 0.542822 2.4:+69331796 MS.gene004219:CDS
GAAAGGACAAATGAAGCTGC+TGG 0.551991 2.4:+69331704 MS.gene004219:CDS
ATCTTTGGACTAAAATGCAA+CGG 0.555620 2.4:+69331783 MS.gene004219:CDS
TACCGTACGAGAGAAATAGT+TGG 0.562274 2.4:-69329002 None:intergenic
GGGCACTGAAGGTGTTGGAA+AGG 0.563729 2.4:+69330436 MS.gene004219:CDS
ATAAGGACAACATTAGTTGA+CGG 0.572638 2.4:-69331284 None:intergenic
AGTATCACATGGCGGTGACG+AGG 0.572977 2.4:+69330467 MS.gene004219:CDS
GCTTCTTCATCTATGGGAGG+AGG 0.576690 2.4:-69331311 None:intergenic
TGTGAGTCTAGAATCTATGC+AGG 0.579365 2.4:+69332163 MS.gene004219:CDS
TGTTCCTCTTCATGAGTACA+AGG 0.584094 2.4:+69330575 MS.gene004219:CDS
TTAGTCCAAAGATCAATGAA+AGG 0.592788 2.4:-69331773 None:intergenic
AAAAGGAGACAATGGAGCGA+AGG 0.597580 2.4:-69330602 None:intergenic
ACCCCATCCTCCAACACCAA+AGG 0.599552 2.4:-69328969 None:intergenic
TGGTGTTCAGAGGGTATCGT+GGG 0.600203 2.4:+69330400 MS.gene004219:CDS
GGTTTGCATCTCACTAATGG+AGG 0.604059 2.4:+69332092 MS.gene004219:CDS
TTGTCCTTGTACTCATGAAG+AGG 0.610871 2.4:-69330579 None:intergenic
TGATCCAAATGATCCACTGA+AGG 0.615047 2.4:+69332211 MS.gene004219:CDS
TAACACACGATGGGCACTGA+AGG 0.617976 2.4:+69330425 MS.gene004219:CDS
AGGCTGATGTGGTGTTCAGA+GGG 0.618365 2.4:+69330391 MS.gene004219:intron
ACGATGGGCACTGAAGGTGT+TGG 0.625863 2.4:+69330431 MS.gene004219:CDS
GTGGTGTTCAGAGGGTATCG+TGG 0.629790 2.4:+69330399 MS.gene004219:CDS
GGATCAATATCAGCTAAAAG+GGG 0.632239 2.4:-69332194 None:intergenic
TGCTAAAGCATGCATTTCTG+TGG 0.632567 2.4:+69331733 MS.gene004219:CDS
TAGGCTGATGTGGTGTTCAG+AGG 0.635589 2.4:+69330390 MS.gene004219:intron
TTGTTTGTGAACAATCCGGA+GGG 0.641948 2.4:+69331674 MS.gene004219:CDS
TCGTGGGTATAACACACGAT+GGG 0.646142 2.4:+69330416 MS.gene004219:CDS
TGTTTGTGAACAATCCGGAG+GGG 0.648290 2.4:+69331675 MS.gene004219:CDS
GTATCACATGGCGGTGACGA+GGG 0.648679 2.4:+69330468 MS.gene004219:CDS
CGTGCTTCTTCATCTATGGG+AGG 0.659087 2.4:-69331314 None:intergenic
TTTGTTTGTGAACAATCCGG+AGG 0.659446 2.4:+69331673 MS.gene004219:CDS
GTTTGCATCTCACTAATGGA+GGG 0.661505 2.4:+69332093 MS.gene004219:CDS
GATCAATATCAGCTAAAAGG+GGG 0.672744 2.4:-69332193 None:intergenic
ATCGTGGGTATAACACACGA+TGG 0.679127 2.4:+69330415 MS.gene004219:CDS
GCTCGTGTGATAGGCTGATG+TGG 0.704764 2.4:+69330380 MS.gene004219:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAAAATAAATAAA+AGG + chr2.4:69329945-69329964 MS.gene004219:intron 0.0%
!!! AATTAAAAAAAAATTATTTT+TGG - chr2.4:69330229-69330248 None:intergenic 0.0%
!! GTATAAATAAATCATATAAT+TGG + chr2.4:69331225-69331244 MS.gene004219:intron 10.0%
!!! AATAAATAAAAGGTTAATTT+TGG + chr2.4:69329955-69329974 MS.gene004219:intron 10.0%
!!! CATTAGTATTTTAAATTAAA+TGG - chr2.4:69331908-69331927 None:intergenic 10.0%
!!! CTTTAAAATGATTTTTTTTT+TGG + chr2.4:69329648-69329667 MS.gene004219:intron 10.0%
!!! TTATTAAAATTAAAAGTGTT+TGG + chr2.4:69329480-69329499 MS.gene004219:intron 10.0%
!! AAAATTCTAAACATTTAAAG+TGG - chr2.4:69331159-69331178 None:intergenic 15.0%
!! ATAAATATAAATGACGTAAA+AGG + chr2.4:69329527-69329546 MS.gene004219:intron 15.0%
!! ATATGATTTATTTATACAAG+AGG - chr2.4:69331222-69331241 None:intergenic 15.0%
!! ATTAAATATGCAAAGATAAT+TGG + chr2.4:69331953-69331972 MS.gene004219:intron 15.0%
!! TATTATATGAATTATGAACA+AGG + chr2.4:69329799-69329818 MS.gene004219:intron 15.0%
!!! AAAAATCTTATTTTAAAAGC+CGG - chr2.4:69330730-69330749 None:intergenic 15.0%
!!! AATGTTTTTATTTTTCGTTA+TGG + chr2.4:69329150-69329169 MS.gene004219:intron 15.0%
!!! ACATTTGATTTATCATATAA+TGG - chr2.4:69330997-69331016 None:intergenic 15.0%
!!! CTTAATTTTGTTTTTTCTTT+AGG + chr2.4:69331074-69331093 MS.gene004219:intron 15.0%
!! AAATCAAATGTAGAAATAGT+AGG + chr2.4:69330936-69330955 MS.gene004219:intron 20.0%
!! AAATCAAATGTAGAAATTGT+AGG + chr2.4:69331006-69331025 MS.gene004219:intron 20.0%
!! AACTTATTAATTAGTGAGTT+GGG + chr2.4:69330873-69330892 MS.gene004219:intron 20.0%
!! AAGAAAACCTAATAAAAACA+AGG - chr2.4:69331574-69331593 None:intergenic 20.0%
!! AGAAAACCTAATAAAAACAA+GGG - chr2.4:69331573-69331592 None:intergenic 20.0%
!! ATAAAAAAACTAAGAGATGA+AGG + chr2.4:69332140-69332159 MS.gene004219:CDS 20.0%
!! TAAAAACAATGACAAAATCA+AGG - chr2.4:69331368-69331387 None:intergenic 20.0%
!! TAAAATACTAATGAACATGA+TGG + chr2.4:69331915-69331934 MS.gene004219:intron 20.0%
!! TAAACATAAATGAAGAGTTA+AGG + chr2.4:69330701-69330720 MS.gene004219:intron 20.0%
!! TAATAAATAAACCCGTTATT+AGG - chr2.4:69330781-69330800 None:intergenic 20.0%
!! TATAGAAGTTAATCATACTT+AGG + chr2.4:69331625-69331644 MS.gene004219:intron 20.0%
!! TTTCAATCTATTACAAAATC+TGG - chr2.4:69329103-69329122 None:intergenic 20.0%
!!! AACAAAGAAGGTAAAATTTT+TGG - chr2.4:69331842-69331861 None:intergenic 20.0%
!!! AAGAAAATGTTTCCATTTTT+GGG - chr2.4:69331432-69331451 None:intergenic 20.0%
!!! TAAGAAAATGTTTCCATTTT+TGG - chr2.4:69331433-69331452 None:intergenic 20.0%
!!! TACTTTCTCAAAAATCATTT+TGG - chr2.4:69330301-69330320 None:intergenic 20.0%
!!! TGATTTTGTCATTGTTTTTA+GGG + chr2.4:69331368-69331387 MS.gene004219:intron 20.0%
!!! TTGATTTTGTCATTGTTTTT+AGG + chr2.4:69331367-69331386 MS.gene004219:intron 20.0%
!!! TTTGTTTTTTCTTTAGGTTT+AGG + chr2.4:69331080-69331099 MS.gene004219:intron 20.0%
!!! TTTTGGAAAACATCTTTAAA+AGG + chr2.4:69329665-69329684 MS.gene004219:intron 20.0%
! AAAACATGTGAAACAGATAT+AGG - chr2.4:69330157-69330176 None:intergenic 25.0%
! AAATTCCTTTCATTGATCTT+TGG + chr2.4:69331768-69331787 MS.gene004219:CDS 25.0%
! AGAAAAAACATGAAATGACA+TGG - chr2.4:69329064-69329083 None:intergenic 25.0%
! AGTGAACAAGAAATCTTTAA+AGG + chr2.4:69329838-69329857 MS.gene004219:intron 25.0%
! AGTTAATGAAACCTAATAAC+GGG + chr2.4:69330767-69330786 MS.gene004219:intron 25.0%
! CACTATGGAAAATATTTGTT+AGG + chr2.4:69329322-69329341 MS.gene004219:intron 25.0%
! GAAATGTGCAAATTTGATAT+TGG + chr2.4:69330850-69330869 MS.gene004219:intron 25.0%
! GAACTTATTAATTAGTGAGT+TGG + chr2.4:69330872-69330891 MS.gene004219:intron 25.0%
! GAGTTAATGAAACCTAATAA+CGG + chr2.4:69330766-69330785 MS.gene004219:intron 25.0%
! TAACAATGACTAATCTATGT+TGG - chr2.4:69330180-69330199 None:intergenic 25.0%
! TAAGAGAAGATAAACAAAGA+AGG - chr2.4:69331854-69331873 None:intergenic 25.0%
! TATCTTCTCTTACAATACTT+TGG + chr2.4:69331862-69331881 MS.gene004219:intron 25.0%
! TTACCAAGTTACCTTAAAAA+AGG - chr2.4:69330622-69330641 None:intergenic 25.0%
! TTATTTGTTTGTGAACAATC+CGG + chr2.4:69331670-69331689 MS.gene004219:CDS 25.0%
!! ATGCTATGGTATTATAGTTT+TGG + chr2.4:69332032-69332051 MS.gene004219:intron 25.0%
!! GGTGACATTTTACATTTTAA+TGG - chr2.4:69330976-69330995 None:intergenic 25.0%
!! TCATCAAAATTGACTTTGTA+TGG - chr2.4:69331990-69332009 None:intergenic 25.0%
!!! ACATTTCCCTTGTTTTTATT+AGG + chr2.4:69331564-69331583 MS.gene004219:intron 25.0%
!!! CTTTGAGTTTTTCATTTTAG+AGG - chr2.4:69330907-69330926 None:intergenic 25.0%
!!! TCAATTTTTAGTTCACACTA+TGG + chr2.4:69329307-69329326 MS.gene004219:intron 25.0%
!!! TTTCATGTTTTTTCTCTTGA+TGG + chr2.4:69329069-69329088 MS.gene004219:intron 25.0%
AAAAAACAGCAACATTCTTG+TGG - chr2.4:69329913-69329932 None:intergenic 30.0%
AAGAGGCTCAAATATCTTAT+CGG - chr2.4:69329269-69329288 None:intergenic 30.0%
AATCAAAGTATGTGATGCTA+TGG + chr2.4:69332018-69332037 MS.gene004219:intron 30.0%
ATAAGGACAACATTAGTTGA+CGG - chr2.4:69331287-69331306 None:intergenic 30.0%
ATCTTTGGACTAAAATGCAA+CGG + chr2.4:69331783-69331802 MS.gene004219:CDS 30.0%
ATTTGAGCCTCTTAAATATG+TGG + chr2.4:69329276-69329295 MS.gene004219:intron 30.0%
TACCTTAAAAAAGGAGACAA+TGG - chr2.4:69330613-69330632 None:intergenic 30.0%
TCACCGAAAAGATATATTTG+TGG - chr2.4:69328932-69328951 None:intergenic 30.0%
TGGATCAATATCAGCTAAAA+GGG - chr2.4:69332198-69332217 None:intergenic 30.0%
TGTTAACTTAAATGCAGTGA+CGG + chr2.4:69332071-69332090 MS.gene004219:intron 30.0%
TTAGTCCAAAGATCAATGAA+AGG - chr2.4:69331776-69331795 None:intergenic 30.0%
TTGATAGAATAAGACGTGTT+TGG - chr2.4:69330073-69330092 None:intergenic 30.0%
TTGGATCAATATCAGCTAAA+AGG - chr2.4:69332199-69332218 None:intergenic 30.0%
! AGGCCACAAATATATCTTTT+CGG + chr2.4:69328926-69328945 MS.gene004219:CDS 30.0%
! TGAAAAACAGAGCATCTATT+AGG - chr2.4:69330552-69330571 None:intergenic 30.0%
! TTGCTTTGACAGTTTTCTTT+GGG + chr2.4:69330508-69330527 MS.gene004219:CDS 30.0%
!!! TCTCCTTTTTTAAGGTAACT+TGG + chr2.4:69330616-69330635 MS.gene004219:intron 30.0%
AAACAGATATAGGCTGTGTT+TGG - chr2.4:69330147-69330166 None:intergenic 35.0%
AAATGAAGAGTTAAGGAGAC+CGG + chr2.4:69330708-69330727 MS.gene004219:intron 35.0%
AAATGACATGGAAACATGGT+TGG - chr2.4:69329052-69329071 None:intergenic 35.0%
AACCGTAATATATGACAACC+TGG + chr2.4:69329862-69329881 MS.gene004219:intron 35.0%
AAGAAAACTGTCAAAGCAAC+AGG - chr2.4:69330507-69330526 None:intergenic 35.0%
ACCCAGGTTGTCATATATTA+CGG - chr2.4:69329867-69329886 None:intergenic 35.0%
ACCGTAATATATGACAACCT+GGG + chr2.4:69329863-69329882 MS.gene004219:intron 35.0%
AGAGGCTCAAATATCTTATC+GGG - chr2.4:69329268-69329287 None:intergenic 35.0%
AGGGAATCAATTTGTGTTTG+AGG + chr2.4:69332112-69332131 MS.gene004219:CDS 35.0%
ATATATGACAACCTGGGTTA+TGG + chr2.4:69329869-69329888 MS.gene004219:intron 35.0%
CAAAAAACGCTATAAGCTAG+TGG + chr2.4:69330034-69330053 MS.gene004219:intron 35.0%
CATGAAATGACATGGAAACA+TGG - chr2.4:69329056-69329075 None:intergenic 35.0%
CATGTTAAGCAACCCAAAAA+TGG + chr2.4:69331417-69331436 MS.gene004219:intron 35.0%
CTCTTGACCACATATTTAAG+AGG - chr2.4:69329286-69329305 None:intergenic 35.0%
GAAAGGAATTTGACACTCAT+TGG - chr2.4:69331759-69331778 None:intergenic 35.0%
GATCAATATCAGCTAAAAGG+GGG - chr2.4:69332196-69332215 None:intergenic 35.0%
GATGGGAAAAAGTTTACTTG+AGG + chr2.4:69331813-69331832 MS.gene004219:CDS 35.0%
GGATCAATATCAGCTAAAAG+GGG - chr2.4:69332197-69332216 None:intergenic 35.0%
TATATGACAACCTGGGTTAT+GGG + chr2.4:69329870-69329889 MS.gene004219:intron 35.0%
TTGCTTAACATGTGCCTTAA+GGG - chr2.4:69331409-69331428 None:intergenic 35.0%
! CTTTTGAATTTCAGAAGCGA+TGG + chr2.4:69331261-69331280 MS.gene004219:intron 35.0%
! TAAGCTAGCTTATAGCTTGT+TGG - chr2.4:69330115-69330134 None:intergenic 35.0%
! TGCTTTGACAGTTTTCTTTG+GGG + chr2.4:69330509-69330528 MS.gene004219:CDS 35.0%
!! AAGTAAACTTTTTCCCATCC+AGG - chr2.4:69331812-69331831 None:intergenic 35.0%
!! AATCATTAACTTGTGCCTTG+AGG + chr2.4:69331531-69331550 MS.gene004219:intron 35.0%
!! AGTAAACTTTTTCCCATCCA+GGG - chr2.4:69331811-69331830 None:intergenic 35.0%
!! ATCATTAACTTGTGCCTTGA+GGG + chr2.4:69331532-69331551 MS.gene004219:intron 35.0%
!! GTTGCTTTGACAGTTTTCTT+TGG + chr2.4:69330507-69330526 MS.gene004219:CDS 35.0%
!!! CTCCATTGTCTCCTTTTTTA+AGG + chr2.4:69330608-69330627 MS.gene004219:CDS 35.0%
AAGTCGTGCTTCTTCATCTA+TGG - chr2.4:69331321-69331340 None:intergenic 40.0%
AATGCCTTCAGTGGATCATT+TGG - chr2.4:69332218-69332237 None:intergenic 40.0%
AGTCGTGCTTCTTCATCTAT+GGG - chr2.4:69331320-69331339 None:intergenic 40.0%
ATCAAATGAGCTCGTGTGAT+AGG + chr2.4:69330371-69330390 MS.gene004219:intron 40.0%
GTTGCTTAACATGTGCCTTA+AGG - chr2.4:69331410-69331429 None:intergenic 40.0%
GTTTGCATCTCACTAATGGA+GGG + chr2.4:69332093-69332112 MS.gene004219:CDS 40.0%
TACCGTACGAGAGAAATAGT+TGG - chr2.4:69329005-69329024 None:intergenic 40.0%
TAGGAGAGAGAATCCACTAT+GGG + chr2.4:69329341-69329360 MS.gene004219:intron 40.0%
TCGATCACTGAAGAATCCTT+TGG + chr2.4:69328953-69328972 MS.gene004219:CDS 40.0%
TGATCCAAATGATCCACTGA+AGG + chr2.4:69332211-69332230 MS.gene004219:CDS 40.0%
TGCTAAAGCATGCATTTCTG+TGG + chr2.4:69331733-69331752 MS.gene004219:CDS 40.0%
TGTTCCTCTTCATGAGTACA+AGG + chr2.4:69330575-69330594 MS.gene004219:CDS 40.0%
TTAGGAGAGAGAATCCACTA+TGG + chr2.4:69329340-69329359 MS.gene004219:intron 40.0%
TTGTCCTTGTACTCATGAAG+AGG - chr2.4:69330582-69330601 None:intergenic 40.0%
TTGTTTGTGAACAATCCGGA+GGG + chr2.4:69331674-69331693 MS.gene004219:CDS 40.0%
TTTACCAAACACACCCATAG+TGG - chr2.4:69329357-69329376 None:intergenic 40.0%
TTTGTTTGTGAACAATCCGG+AGG + chr2.4:69331673-69331692 MS.gene004219:CDS 40.0%
! ACTGAAGAATCCTTTGGTGT+TGG + chr2.4:69328959-69328978 MS.gene004219:CDS 40.0%
! AGGGTTTTTCCAGTATCACA+TGG + chr2.4:69330456-69330475 MS.gene004219:CDS 40.0%
! GTTTTTCCAGTATCACATGG+CGG + chr2.4:69330459-69330478 MS.gene004219:CDS 40.0%
! TAGATGAAGAAGCACGACTT+AGG + chr2.4:69331321-69331340 MS.gene004219:CDS 40.0%
! TGCCAACTATTTCTCTCGTA+CGG + chr2.4:69329000-69329019 MS.gene004219:CDS 40.0%
! TGTGAGTCTAGAATCTATGC+AGG + chr2.4:69332163-69332182 MS.gene004219:CDS 40.0%
!! AATGTTAACTTGTGCCCTCA+AGG - chr2.4:69331549-69331568 None:intergenic 40.0%
!! CATGTTAACTTGTGCCCTTA+AGG + chr2.4:69331392-69331411 MS.gene004219:intron 40.0%
AAAAGGAGACAATGGAGCGA+AGG - chr2.4:69330605-69330624 None:intergenic 45.0%
ACTAAAATGCAACGGTTCCC+TGG + chr2.4:69331791-69331810 MS.gene004219:CDS 45.0%
ATCGTGGGTATAACACACGA+TGG + chr2.4:69330415-69330434 MS.gene004219:CDS 45.0%
CAAAGCAACAGGAGCAGTTT+CGG - chr2.4:69330496-69330515 None:intergenic 45.0%
CTATGGGAGGAGGAGTTATA+AGG - chr2.4:69331304-69331323 None:intergenic 45.0%
GAAAGGACAAATGAAGCTGC+TGG + chr2.4:69331704-69331723 MS.gene004219:CDS 45.0%
GAATCCACTATGGGTGTGTT+TGG + chr2.4:69329350-69329369 MS.gene004219:intron 45.0%
GACGGTTTGCATCTCACTAA+TGG + chr2.4:69332089-69332108 MS.gene004219:CDS 45.0%
GCAGCTTCATTTGTCCTTTC+AGG - chr2.4:69331704-69331723 None:intergenic 45.0%
GGTTTGCATCTCACTAATGG+AGG + chr2.4:69332092-69332111 MS.gene004219:CDS 45.0%
TCGTGGGTATAACACACGAT+GGG + chr2.4:69330416-69330435 MS.gene004219:CDS 45.0%
TGTTTGTGAACAATCCGGAG+GGG + chr2.4:69331675-69331694 MS.gene004219:CDS 45.0%
!! AATCCTTTGGTGTTGGAGGA+TGG + chr2.4:69328966-69328985 MS.gene004219:CDS 45.0%
!! ATCCTTTGGTGTTGGAGGAT+GGG + chr2.4:69328967-69328986 MS.gene004219:CDS 45.0%
!! GAAGAATCCTTTGGTGTTGG+AGG + chr2.4:69328962-69328981 MS.gene004219:CDS 45.0%
!!! TATTTTTTAATTTCAATTAT+AGG + chr2.4:69331196-69331215 MS.gene004219:intron 5.0%
AAATGCAACGGTTCCCTGGA+TGG + chr2.4:69331795-69331814 MS.gene004219:CDS 50.0%
AATGCAACGGTTCCCTGGAT+GGG + chr2.4:69331796-69331815 MS.gene004219:CDS 50.0%
ACCTGGGTTATGGGTAAACG+TGG + chr2.4:69329879-69329898 MS.gene004219:intron 50.0%
AGGCTGATGTGGTGTTCAGA+GGG + chr2.4:69330391-69330410 MS.gene004219:intron 50.0%
CGTGCTTCTTCATCTATGGG+AGG - chr2.4:69331317-69331336 None:intergenic 50.0%
GCTTCTTCATCTATGGGAGG+AGG - chr2.4:69331314-69331333 None:intergenic 50.0%
TAGGCTGATGTGGTGTTCAG+AGG + chr2.4:69330390-69330409 MS.gene004219:intron 50.0%
TCCACGTTTACCCATAACCC+AGG - chr2.4:69329883-69329902 None:intergenic 50.0%
!! GGCACTGAAGGTGTTGGAAA+GGG + chr2.4:69330437-69330456 MS.gene004219:CDS 50.0%
!! TAACACACGATGGGCACTGA+AGG + chr2.4:69330425-69330444 MS.gene004219:CDS 50.0%
!! TCCTTTGGTGTTGGAGGATG+GGG + chr2.4:69328968-69328987 MS.gene004219:CDS 50.0%
!! TGGTGTTCAGAGGGTATCGT+GGG + chr2.4:69330400-69330419 MS.gene004219:CDS 50.0%
ACCCCATCCTCCAACACCAA+AGG - chr2.4:69328972-69328991 None:intergenic 55.0%
AGTATCACATGGCGGTGACG+AGG + chr2.4:69330467-69330486 MS.gene004219:CDS 55.0%
GCTCGTGTGATAGGCTGATG+TGG + chr2.4:69330380-69330399 MS.gene004219:intron 55.0%
GTATCACATGGCGGTGACGA+GGG + chr2.4:69330468-69330487 MS.gene004219:CDS 55.0%
TCGTCACCGCCATGTGATAC+TGG - chr2.4:69330468-69330487 None:intergenic 55.0%
!! ACGATGGGCACTGAAGGTGT+TGG + chr2.4:69330431-69330450 MS.gene004219:CDS 55.0%
!! GGGCACTGAAGGTGTTGGAA+AGG + chr2.4:69330436-69330455 MS.gene004219:CDS 55.0%
!! GTGGTGTTCAGAGGGTATCG+TGG + chr2.4:69330399-69330418 MS.gene004219:CDS 55.0%
ATCCGGAGGGGCTTCCTGAA+AGG + chr2.4:69331687-69331706 MS.gene004219:CDS 60.0%
GTCCTTTCAGGAAGCCCCTC+CGG - chr2.4:69331692-69331711 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 69328917 69332244 69328917 ID=MS.gene004219
chr2.4 mRNA 69328917 69332244 69328917 ID=MS.gene004219.t1;Parent=MS.gene004219
chr2.4 exon 69328917 69329021 69328917 ID=MS.gene004219.t1.exon1;Parent=MS.gene004219.t1
chr2.4 CDS 69328917 69329021 69328917 ID=cds.MS.gene004219.t1;Parent=MS.gene004219.t1
chr2.4 exon 69330393 69330629 69330393 ID=MS.gene004219.t1.exon2;Parent=MS.gene004219.t1
chr2.4 CDS 69330393 69330629 69330393 ID=cds.MS.gene004219.t1;Parent=MS.gene004219.t1
chr2.4 exon 69331275 69331342 69331275 ID=MS.gene004219.t1.exon3;Parent=MS.gene004219.t1
chr2.4 CDS 69331275 69331342 69331275 ID=cds.MS.gene004219.t1;Parent=MS.gene004219.t1
chr2.4 exon 69331670 69331834 69331670 ID=MS.gene004219.t1.exon4;Parent=MS.gene004219.t1
chr2.4 CDS 69331670 69331834 69331670 ID=cds.MS.gene004219.t1;Parent=MS.gene004219.t1
chr2.4 exon 69332088 69332244 69332088 ID=MS.gene004219.t1.exon5;Parent=MS.gene004219.t1
chr2.4 CDS 69332088 69332244 69332088 ID=cds.MS.gene004219.t1;Parent=MS.gene004219.t1
Gene Sequence

>MS.gene004219

ATGACTAAGAGGCCACAAATATATCTTTTCGGTGATTCGATCACTGAAGAATCCTTTGGTGTTGGAGGATGGGGTGCTTCTCTTGCCAACTATTTCTCTCGTACGGTAATAATTCATTCTTGCTTTAAGTGACCAACCATGTTTCCATGTCATTTCATGTTTTTTCTCTTGATGGTTATTTTTCCAGATTTTGTAATAGATTGAAATGAAAGTGATTTTAAATAAAAATAAACAATGTTTTTATTTTTCGTTATGGTTTACAAATCATTCAAGTTGAAAAACTATACATGTTAAATTAGATTTTCATTAATATGATTTAAAGAAAGATAGATTCAGCAATCGATTGATCCCGATAAGATATTTGAGCCTCTTAAATATGTGGTCAAGAGTTCAATTTTTAGTTCACACTATGGAAAATATTTGTTAGGAGAGAGAATCCACTATGGGTGTGTTTGGTAAAAATAAGCTATAAGCTAGCTGATAGCTGATAAGCTAGCTGATAGATGAAAAGCTAGCTTATAGCTGATGACTGATAGCTGATAGCTTATAGCTTAAAAGTTAGCTTATTAAAATTAAAAGTGTTTGGTAAAATTAGCTGTTGAAGTGACTGATAAATATAAATGACGTAAAAGGACGTGTTTGTTTAATTTTTAATATAAATCATATATATAATTTTTATTACTTAACGTATTTCTTTTTAAAGATTTAAATATATTACAAATTATTTTCATCTTTAAAATGATTTTTTTTTTGGAAAACATCTTTAAAAGGATAAATATATATGAATTCAATCGATATTTTCTATTCGACAAAACTTTTTTTTATGAATTTTATTTAACTAAATTTGCTTCACTGTTTATTGTAAATTTTTATAAAATTATTTATTATATGAATTATGAACAAGGTAAAAAAAACAAAAAAAGTGAACAAGAAATCTTTAAAGGAAACCGTAATATATGACAACCTGGGTTATGGGTAAACGTGGACAGTTTACCACAAGAATGTTGCTGTTTTTTTTTATTATAAAAAATAAAATAAAATAAATAAAAGGTTAATTTTGGAAGAAAATATAAAAAGCTACAAGCTAAAAGCTCAAAAGCTACTTGAAATAGCTCTTCAAAAAACGCTATAAGCTAGTGGAAAAAGCTTTTTACCAAACACGTCTTATTCTATCAAAACAAGCTTATAAGCTAGTCCAACAAGCTATAAGCTAGCTTATTTGTGTTACCAAACACAGCCTATATCTGTTTCACATGTTTTCCAACATAGATTAGTCATTGTTAAACAACTTCGTACTAATTTCATGTAACCAAAAATAATTTTTTTTTAATTTTCAAAAACTAATTAAAGTAATTGTTACATTTTCAAACTTTTTTTTTTACCAAAATGATTTTTGAGAAAGTACACACAAACATAATTAAAAGATCAAAAGTGATTTTCTCAAAATATTTTTAATCAAATGAGCTCGTGTGATAGGCTGATGTGGTGTTCAGAGGGTATCGTGGGTATAACACACGATGGGCACTGAAGGTGTTGGAAAGGGTTTTTCCAGTATCACATGGCGGTGACGAGGGAACCGAAACTGCTCCTGTTGCTTTGACAGTTTTCTTTGGGGCTAATGATGCTTGTCTTCCTAATAGATGCTCTGTTTTTCAACATGTTCCTCTTCATGAGTACAAGGACAACCTTCGCTCCATTGTCTCCTTTTTTAAGGTAACTTGGTAACAAGTTCTGATTTCTTCAATTATTTATTTTTGTTTATTATTGTTCATTCATAAGAGAGATAAACATAAATGAAGAGTTAAGGAGACCGGCTTTTAAAATAAGATTTTTTTTTTTTTAAAATCAAGAGTTAATGAAACCTAATAACGGGTTTATTTATTAAAAAAAATCTATCATGTGCAACTTTGATATTAGAACTTTTTTTTTGAAAGAAATGTGCAAATTTGATATTGGAACTTATTAATTAGTGAGTTGGGATCTGATTCCTCTAAAATGAAAAACTCAAAGTAAAATATTAAATCAAATGTAGAAATAGTAGGTTCATGCAAATCAACCATTAAAATGTAAAATGTCACCATTATATGATAAATCAAATGTAGAAATTGTAGGTTCATGCAAATCATAAGTTTGTTATTAACAACTCTTAACTAGCTTCTTAATTTTGTTTTTTCTTTAGGTTTAGGCTCTCTTTTGTTCATGAAAAAACAACTTTTTATTTTCTGACTTTACTCTTTAGCCACTTTAAATGTTTAGAATTTTGTTTGATGACGCTTAAATATTTTTTAATTTCAATTATAGGCCTCTTGTATAAATAAATCATATAATTGGTTACTGCAACTTTCTTTTGAATTTCAGAAGCGATGGCCGTCAACTAATGTTGTCCTTATAACTCCTCCTCCCATAGATGAAGAAGCACGACTTAGGTAATGTTTCATTGACTTTTTTCCTTGATTTTGTCATTGTTTTTAGGGTCATGTTAACTTGTGCCCTTAAGGCACATGTTAAGCAACCCAAAAATGGAAACATTTTCTTAAATTTTGTGCATTCAATTATTGAAAAGTAAAAAAGTTAAAGTCAAATGCATTAATTACAATAGAATATTTCTATTTTGAATCATTAACTTGTGCCTTGAGGGCACAAGTTAACATTTCCCTTGTTTTTATTAGGTTTTCTTTGTTTGTGACACACACACACACGTATAATTCTATAGAAGTTAATCATACTTAGGAATTTCTAAAAATTTATTGTAGTTATTTGTTTGTGAACAATCCGGAGGGGCTTCCTGAAAGGACAAATGAAGCTGCTGGTGAATATGCTAAAGCATGCATTTCTGTGGCCAATGAGTGTCAAATTCCTTTCATTGATCTTTGGACTAAAATGCAACGGTTCCCTGGATGGGAAAAAGTTTACTTGAGGTTTCCAAAAATTTTACCTTCTTTGTTTATCTTCTCTTACAATACTTTGGAATCTTGATATTAGTATTTACCATTTAATTTAAAATACTAATGAACATGATGGCTTTGTTATAGTTTGATTAAATATGCAAAGATAATTGGAAAATCAATTACCATACAAAGTCAATTTTGATGATGCTAATTAATCAAAGTATGTGATGCTATGGTATTATAGTTTTGGTTTTGATATATGATTATGTTAACTTAAATGCAGTGACGGTTTGCATCTCACTAATGGAGGGAATCAATTTGTGTTTGAGGAAGTAATAAAAAAACTAAGAGATGAAGGTGTGAGTCTAGAATCTATGCAGGTTGATCTCCCCCTTTTAGCTGATATTGATCCAAATGATCCACTGAAGGCATTTTTGTAG
Protein sequence

>MS.gene004219.t1

MTKRPQIYLFGDSITEESFGVGGWGASLANYFSRTADVVFRGYRGYNTRWALKVLERVFPVSHGGDEGTETAPVALTVFFGANDACLPNRCSVFQHVPLHEYKDNLRSIVSFFKKRWPSTNVVLITPPPIDEEARLSYLFVNNPEGLPERTNEAAGEYAKACISVANECQIPFIDLWTKMQRFPGWEKVYLSDGLHLTNGGNQFVFEEVIKKLRDEGVSLESMQVDLPLLADIDPNDPLKAFL