AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004220.t1 MTR_2g015500 96.708 243 8 0 1 243 1 243 3.08e-180 494
MS.gene004220.t1 MTR_2g015510 84.298 242 36 1 2 243 3 242 3.81e-156 433
MS.gene004220.t1 MTR_2g015480 67.249 229 58 4 12 239 65 277 2.09e-98 290
MS.gene004220.t1 MTR_4g105680 45.249 221 112 2 25 242 51 265 5.05e-58 185
MS.gene004220.t1 MTR_7g068550 41.981 212 113 3 4 215 6 207 4.49e-54 175
MS.gene004220.t1 MTR_4g016510 38.136 236 134 4 4 235 6 233 1.09e-51 169
MS.gene004220.t1 MTR_7g068530 38.075 239 134 4 4 238 6 234 1.80e-50 165
MS.gene004220.t1 MTR_7g068460 38.318 214 122 4 2 215 31 234 1.25e-47 159
MS.gene004220.t1 MTR_7g068500 37.850 214 123 4 2 215 30 233 6.79e-46 154
MS.gene004220.t1 MTR_7g068470 36.620 213 123 6 4 215 2 203 8.21e-44 148
MS.gene004220.t1 MTR_7g068450 34.694 245 140 5 4 239 2 235 1.45e-43 148
MS.gene004220.t1 MTR_7g068470 37.089 213 118 7 4 215 2 199 2.38e-43 147
MS.gene004220.t1 MTR_3g070460 36.449 214 124 5 4 215 6 209 1.60e-42 145
MS.gene004220.t1 MTR_4g016500 32.484 157 65 5 33 189 277 392 4.11e-18 83.2
MS.gene004220.t1 MTR_7g068520 50.847 59 28 1 4 62 3 60 2.28e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004220.t1 AT5G45920 68.880 241 68 2 4 242 3 238 2.57e-122 348
MS.gene004220.t1 AT5G45920 68.880 241 68 2 4 242 3 238 2.57e-122 348
MS.gene004220.t1 AT5G45920 72.043 186 45 2 4 187 3 183 1.90e-96 280
MS.gene004220.t1 AT5G45920 72.043 186 45 2 4 187 3 183 1.90e-96 280
MS.gene004220.t1 AT5G62930 52.066 242 107 2 4 242 2 237 2.21e-83 249
MS.gene004220.t1 AT5G62930 52.066 242 107 2 4 242 28 263 7.88e-83 248
MS.gene004220.t1 AT5G62930 49.438 178 87 1 68 242 2 179 8.00e-54 172
MS.gene004220.t1 AT2G38180 38.627 233 129 4 4 232 6 228 9.91e-48 160
MS.gene004220.t1 AT3G11210 35.246 244 139 6 4 241 6 236 1.57e-45 153

Find 68 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTACAGAAATGCCTTTA+AGG 0.118994 2.4:-69324796 None:intergenic
GCAGCTTCATTAGTCCTTTC+AGG 0.209931 2.4:-69324268 None:intergenic
TGACCCTAATGACCCCTTAA+AGG 0.261070 2.4:+69324782 MS.gene004220:CDS
GTCAGGGAACTGTTGCATTT+TGG 0.294369 2.4:-69324359 None:intergenic
CTCCATTGTCTCCTTCTTCA+AGG 0.304012 2.4:+69322992 MS.gene004220:CDS
AATGCCTTTAAGGGGTCATT+AGG 0.307170 2.4:-69324786 None:intergenic
TCCTTTGATGTTGGAGGATG+GGG 0.334235 2.4:+69322227 MS.gene004220:CDS
TACTACAGAAATGCCTTTAA+GGG 0.399937 2.4:-69324795 None:intergenic
CAAAGCAATCGGAGCAGTTT+CGG 0.402911 2.4:-69322877 None:intergenic
ATGCCTTTAAGGGGTCATTA+GGG 0.414468 2.4:-69324785 None:intergenic
GTCCTTTCAGGAAGACCCTC+TGG 0.415276 2.4:-69324256 None:intergenic
CCCTCTGGATTATTCTCAAA+TGG 0.419053 2.4:-69324241 None:intergenic
ATATTCCTTACATTGATCTC+TGG 0.444800 2.4:+69324335 MS.gene004220:CDS
AAGATATATTTGTGGCCTCT+TGG 0.446023 2.4:-69322180 None:intergenic
GAGTCGTGCAACTTCATCAA+TGG 0.457432 2.4:-69324082 None:intergenic
TTGAAAAGCAGAGCATCTAC+TGG 0.476943 2.4:-69322934 None:intergenic
ATCCTTTGATGTTGGAGGAT+GGG 0.480890 2.4:+69322226 MS.gene004220:CDS
AAATGCAACAGTTCCCTGAC+TGG 0.492869 2.4:+69324362 MS.gene004220:CDS
ATGAGGATGACTTTACTTGT+CGG 0.494758 2.4:-69324048 None:intergenic
AGTCGTGCAACTTCATCAAT+GGG 0.507323 2.4:-69324081 None:intergenic
AAGAAAACAGTCAAAGCAAT+CGG 0.513354 2.4:-69322888 None:intergenic
CAGGCAGATGTGGTGCTAAG+AGG 0.513358 2.4:+69322774 MS.gene004220:intron
ACTGAGGAATCCTTTGATGT+TGG 0.516117 2.4:+69322218 MS.gene004220:CDS
AGGCAGATGTGGTGCTAAGA+GGG 0.519656 2.4:+69322775 MS.gene004220:intron
CAACACGAGGTGGGCACTGA+AGG 0.520803 2.4:+69322809 MS.gene004220:CDS
TTGATGAAGTTGCACGACTC+AGG 0.521821 2.4:+69324085 MS.gene004220:CDS
CAATGGGAGGAGGAGTTATG+AGG 0.526221 2.4:-69324065 None:intergenic
AATCCTTTGATGTTGGAGGA+TGG 0.530232 2.4:+69322225 MS.gene004220:CDS
TTGGTCCAGAGATCAATGTA+AGG 0.532044 2.4:-69324340 None:intergenic
CTGTCACCGCCTTGTGACAC+AGG 0.536050 2.4:-69322849 None:intergenic
GAGGAATCCTTTGATGTTGG+AGG 0.547556 2.4:+69322221 MS.gene004220:CDS
TTCTCCTTGTATTCATGTAG+AGG 0.550295 2.4:-69322963 None:intergenic
ATCCAGAGGGTCTTCCTGAA+AGG 0.553423 2.4:+69324254 MS.gene004220:CDS
GTGGTGCTAAGAGGGTATAG+TGG 0.556252 2.4:+69322783 MS.gene004220:CDS
TAGGGTCAATATCAGATACA+AGG 0.556300 2.4:-69324767 None:intergenic
CCTTTGAATTTCAGAAGCGA+TGG 0.564667 2.4:+69324025 MS.gene004220:intron
ACCCCATCCTCCAACATCAA+AGG 0.571101 2.4:-69322228 None:intergenic
TCTCCTTGTATTCATGTAGA+GGG 0.573844 2.4:-69322962 None:intergenic
TGCCAACTATTTCTCACGCA+CGG 0.583852 2.4:+69322259 MS.gene004220:CDS
TACCGTGCGTGAGAAATAGT+TGG 0.585214 2.4:-69322261 None:intergenic
TGTCCCTCTACATGAATACA+AGG 0.587200 2.4:+69322959 MS.gene004220:CDS
GGTATGGGTTACCTTGAAGA+AGG 0.588405 2.4:-69323003 None:intergenic
TGGTGCTAAGAGGGTATAGT+GGG 0.591667 2.4:+69322784 MS.gene004220:CDS
TTCTTTCAAACATGACCAAG+AGG 0.596002 2.4:+69322165 None:intergenic
GAAAGGACTAATGAAGCTGC+TGG 0.598460 2.4:+69324271 MS.gene004220:CDS
GACGGTTTGCATCTAACTAA+CGG 0.606195 2.4:+69324660 MS.gene004220:CDS
GCAACTTCATCAATGGGAGG+AGG 0.607044 2.4:-69324075 None:intergenic
ACTACAGAAATGCCTTTAAG+GGG 0.608956 2.4:-69324794 None:intergenic
GTGTCACAAGGCGGTGACAG+TGG 0.609656 2.4:+69322852 MS.gene004220:CDS
TGAAAAGCAGAGCATCTACT+GGG 0.610569 2.4:-69322933 None:intergenic
TACCTTGAAGAAGGAGACAA+TGG 0.615115 2.4:-69322994 None:intergenic
AGGGTCAATATCAGATACAA+GGG 0.620846 2.4:-69324766 None:intergenic
AGAAGGAGACAATGGAGCAA+AGG 0.621194 2.4:-69322986 None:intergenic
GAGGTGGGCACTGAAGGTGT+TGG 0.628300 2.4:+69322815 MS.gene004220:CDS
GTAAGGAATATGACATTCAG+TGG 0.633665 2.4:-69324323 None:intergenic
CCATTTGAGAATAATCCAGA+GGG 0.637647 2.4:+69324241 MS.gene004220:CDS
TGTCACCGCCTTGTGACACA+GGG 0.640635 2.4:-69322848 None:intergenic
TGCTAGAGCATGCATTGCTG+TGG 0.657710 2.4:+69324300 MS.gene004220:CDS
GGGTCAATATCAGATACAAG+GGG 0.659677 2.4:-69324765 None:intergenic
TAACATGTAACAGGCAGATG+TGG 0.662178 2.4:+69322764 MS.gene004220:intron
GTTTGCATCTAACTAACGGA+GGG 0.673924 2.4:+69324664 MS.gene004220:CDS
TCCATTTGAGAATAATCCAG+AGG 0.677789 2.4:+69324240 MS.gene004220:CDS
CGTGCAACTTCATCAATGGG+AGG 0.679277 2.4:-69324078 None:intergenic
GGTATAGTGGGTACAACACG+AGG 0.696277 2.4:+69322796 MS.gene004220:CDS
TAGTGGGTACAACACGAGGT+GGG 0.715177 2.4:+69322800 MS.gene004220:CDS
GGTTTGCATCTAACTAACGG+AGG 0.720069 2.4:+69324663 MS.gene004220:CDS
ATAGTGGGTACAACACGAGG+TGG 0.732833 2.4:+69322799 MS.gene004220:CDS
GGTCAATATCAGATACAAGG+GGG 0.748357 2.4:-69324764 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATCATATTACAATT+TGG - chr2.4:69322419-69322438 None:intergenic 10.0%
!! AATAAATCATATTACAATTT+GGG - chr2.4:69322418-69322437 None:intergenic 10.0%
!!! ATATTTTTAATTTCAATTCT+AGG + chr2.4:69323964-69323983 MS.gene004220:intron 10.0%
!! ATATCATTATTTAATGCATA+TGG + chr2.4:69324593-69324612 MS.gene004220:intron 15.0%
!! GAATAAAATTAGAGTTTAAT+GGG + chr2.4:69323038-69323057 MS.gene004220:intron 15.0%
!! TATAAACAAAACATATAATG+TGG - chr2.4:69322358-69322377 None:intergenic 15.0%
!! TATGATTTGATATGAATATA+CGG + chr2.4:69324616-69324635 MS.gene004220:intron 15.0%
!! TGAATAAAATTAGAGTTTAA+TGG + chr2.4:69323037-69323056 MS.gene004220:intron 15.0%
!! TTAAACATATAATTCTATAG+AGG + chr2.4:69324176-69324195 MS.gene004220:intron 15.0%
!! AAAATCTAAAAACAGAATGT+GGG - chr2.4:69322674-69322693 None:intergenic 20.0%
!! AATTATACCATTTATACAAG+AGG - chr2.4:69323990-69324009 None:intergenic 20.0%
!! ACTGGAAAAAAGTTTATTTA+AGG + chr2.4:69324380-69324399 MS.gene004220:CDS 20.0%
!! TAAAATCTAAAAACAGAATG+TGG - chr2.4:69322675-69322694 None:intergenic 20.0%
!! TACTTAAATAATCATAACAG+CGG - chr2.4:69323279-69323298 None:intergenic 20.0%
!! TATACCTACATATACATTTA+AGG - chr2.4:69323929-69323948 None:intergenic 20.0%
!! TTATCCTTAAATGTATATGT+AGG + chr2.4:69323922-69323941 MS.gene004220:intron 20.0%
!!! AAGTTTGAATCTTTTTGTAA+TGG + chr2.4:69322700-69322719 MS.gene004220:intron 20.0%
!!! AATTTTATTCAAACTCTGTA+CGG - chr2.4:69323028-69323047 None:intergenic 20.0%
!!! AGTTTGAATCTTTTTGTAAT+GGG + chr2.4:69322701-69322720 MS.gene004220:intron 20.0%
! AAAGAACAATGATCCTTTAT+TGG - chr2.4:69323122-69323141 None:intergenic 25.0%
! AAGAACAATGATCCTTTATT+GGG - chr2.4:69323121-69323140 None:intergenic 25.0%
! AATTATGTTATCAGTTGGTT+TGG + chr2.4:69323705-69323724 MS.gene004220:intron 25.0%
! AATTTGGAGGTTAATACAAT+AGG + chr2.4:69323446-69323465 MS.gene004220:intron 25.0%
! ATCTTTGATCCTTAAAGAAA+GGG - chr2.4:69323812-69323831 None:intergenic 25.0%
! TATCTTTGATCCTTAAAGAA+AGG - chr2.4:69323813-69323832 None:intergenic 25.0%
! TATTATTGATCATACAGTAG+TGG - chr2.4:69323501-69323520 None:intergenic 25.0%
! TCACCAAAAAGATATATTTG+TGG - chr2.4:69322191-69322210 None:intergenic 25.0%
! TCTGCTTATAACATTCTTAA+AGG + chr2.4:69323833-69323852 MS.gene004220:intron 25.0%
! TTATAATGTTTGTAAAGCTG+TGG + chr2.4:69323361-69323380 MS.gene004220:intron 25.0%
!! ATTTTATTCAAACTCTGTAC+GGG - chr2.4:69323027-69323046 None:intergenic 25.0%
!! GTTTGAATCTTTTTGTAATG+GGG + chr2.4:69322702-69322721 MS.gene004220:intron 25.0%
!! TTTACCTTCTTTGTTTATCT+TGG + chr2.4:69324415-69324434 MS.gene004220:intron 25.0%
!!! AAAATAGTTTTGATCTCTTG+TGG + chr2.4:69323178-69323197 MS.gene004220:intron 25.0%
!!! AAATAAACTTTTTTCCAGTC+AGG - chr2.4:69324379-69324398 None:intergenic 25.0%
!!! AATAAACTTTTTTCCAGTCA+GGG - chr2.4:69324378-69324397 None:intergenic 25.0%
!!! TCTTTTGAAAAGGATAAATG+AGG + chr2.4:69322733-69322752 MS.gene004220:intron 25.0%
AAAGCAAAACAAAGCAAGAA+TGG - chr2.4:69322291-69322310 None:intergenic 30.0%
AAAGGATCATTGTTCTTTAG+TGG + chr2.4:69323124-69323143 MS.gene004220:intron 30.0%
AACTGATAACATAATTGCAC+AGG - chr2.4:69323701-69323720 None:intergenic 30.0%
AAGAAAACAGTCAAAGCAAT+CGG - chr2.4:69322891-69322910 None:intergenic 30.0%
ACAATAGGTGATGTTAAATG+TGG + chr2.4:69323461-69323480 MS.gene004220:intron 30.0%
ATAAAAAAGCTGAGAGATGA+AGG + chr2.4:69324711-69324730 MS.gene004220:CDS 30.0%
ATATTCCTTACATTGATCTC+TGG + chr2.4:69324335-69324354 MS.gene004220:CDS 30.0%
ATGAACATTGATCAACCTAT+GGG - chr2.4:69323883-69323902 None:intergenic 30.0%
CATAACTCAAGTCACATATA+TGG - chr2.4:69324536-69324555 None:intergenic 30.0%
GTTGACATTGAGTTTATAAG+TGG + chr2.4:69322467-69322486 MS.gene004220:intron 30.0%
GTTTGTTTGTAACATGTAAC+AGG + chr2.4:69322755-69322774 MS.gene004220:intron 30.0%
TAAGCCAAGATAAACAAAGA+AGG - chr2.4:69324422-69324441 None:intergenic 30.0%
TGTGCAATTATGTTATCAGT+TGG + chr2.4:69323700-69323719 MS.gene004220:intron 30.0%
! AGGCCACAAATATATCTTTT+TGG + chr2.4:69322185-69322204 MS.gene004220:CDS 30.0%
! ATTGCTTTGACTGTTTTCTT+TGG + chr2.4:69322891-69322910 MS.gene004220:CDS 30.0%
! TGGATTTGATAAGTGTGTTT+TGG + chr2.4:69323198-69323217 MS.gene004220:intron 30.0%
! TTGCTTTGACTGTTTTCTTT+GGG + chr2.4:69322892-69322911 MS.gene004220:CDS 30.0%
! TTTATTAGTCTCATCGTGTT+AGG + chr2.4:69322387-69322406 MS.gene004220:intron 30.0%
!! GGTTTGTGTTTCTTTTGAAA+AGG + chr2.4:69322723-69322742 MS.gene004220:intron 30.0%
!!! AAGTTTTTTTTACACTTGCG+TGG + chr2.4:69323857-69323876 MS.gene004220:intron 30.0%
AAGAATGGTCCCTTTCTTTA+AGG + chr2.4:69323800-69323819 MS.gene004220:intron 35.0%
AAGATATATTTGTGGCCTCT+TGG - chr2.4:69322183-69322202 None:intergenic 35.0%
AAGCATGGAATCAAACTCTT+TGG - chr2.4:69323530-69323549 None:intergenic 35.0%
ACTGTGTTGAAACCCAATAA+AGG + chr2.4:69323106-69323125 MS.gene004220:intron 35.0%
AGGGTCAATATCAGATACAA+GGG - chr2.4:69324769-69324788 None:intergenic 35.0%
AGTAATGGCACAAAGAAAGA+AGG - chr2.4:69323673-69323692 None:intergenic 35.0%
AGTGTCTGATCTTGATCAAA+CGG + chr2.4:69322585-69322604 MS.gene004220:intron 35.0%
ATGAGGATGACTTTACTTGT+CGG - chr2.4:69324051-69324070 None:intergenic 35.0%
ATTCATTGGAATCACGCATT+CGG + chr2.4:69322508-69322527 MS.gene004220:intron 35.0%
CATGAACATTGATCAACCTA+TGG - chr2.4:69323884-69323903 None:intergenic 35.0%
CCATTTGAGAATAATCCAGA+GGG + chr2.4:69324241-69324260 MS.gene004220:CDS 35.0%
GAGTTTATAAGTGGTGAAAC+TGG + chr2.4:69322476-69322495 MS.gene004220:intron 35.0%
GTAAGGAATATGACATTCAG+TGG - chr2.4:69324326-69324345 None:intergenic 35.0%
TAGGGTCAATATCAGATACA+AGG - chr2.4:69324770-69324789 None:intergenic 35.0%
TCATGTGAGCTGTTAAGTAT+AGG + chr2.4:69323730-69323749 MS.gene004220:intron 35.0%
TCCATTTGAGAATAATCCAG+AGG + chr2.4:69324240-69324259 MS.gene004220:CDS 35.0%
TCTCCTTGTATTCATGTAGA+GGG - chr2.4:69322965-69322984 None:intergenic 35.0%
TTCTAGGCCTCTTGTATAAA+TGG + chr2.4:69323980-69323999 MS.gene004220:intron 35.0%
TTCTCCTTGTATTCATGTAG+AGG - chr2.4:69322966-69322985 None:intergenic 35.0%
! AGAAAGAAGGCATGATGTTT+AGG - chr2.4:69323660-69323679 None:intergenic 35.0%
! TCTTTCTTTGTGCCATTACT+TGG + chr2.4:69323673-69323692 MS.gene004220:intron 35.0%
! TGCTTTGACTGTTTTCTTTG+GGG + chr2.4:69322893-69322912 MS.gene004220:CDS 35.0%
! TTGATTCCATGCTTTTCTGT+GGG + chr2.4:69323536-69323555 MS.gene004220:intron 35.0%
! TTTGATTCCATGCTTTTCTG+TGG + chr2.4:69323535-69323554 MS.gene004220:intron 35.0%
!! CGACTCAGGTATTGTTTTAT+TGG + chr2.4:69324099-69324118 MS.gene004220:intron 35.0%
AATCCTTTGATGTTGGAGGA+TGG + chr2.4:69322225-69322244 MS.gene004220:CDS 40.0%
AATGGGTATGCACACAATGA+GGG + chr2.4:69323055-69323074 MS.gene004220:intron 40.0%
ACTGAGGAATCCTTTGATGT+TGG + chr2.4:69322218-69322237 MS.gene004220:CDS 40.0%
ACTGGATTCCATGCATTCAT+TGG + chr2.4:69322494-69322513 MS.gene004220:intron 40.0%
AGTCGTGCAACTTCATCAAT+GGG - chr2.4:69324084-69324103 None:intergenic 40.0%
ATAGGCATGCATGTTAGCAA+TGG + chr2.4:69323748-69323767 MS.gene004220:intron 40.0%
ATCCTTTGATGTTGGAGGAT+GGG + chr2.4:69322226-69322245 MS.gene004220:CDS 40.0%
ATTCAAACTCTGTACGGGTA+TGG - chr2.4:69323022-69323041 None:intergenic 40.0%
ATTGCACAGGAACCAAGTAA+TGG - chr2.4:69323688-69323707 None:intergenic 40.0%
CAAATTCCTGAAATCCCACA+GGG - chr2.4:69323432-69323451 None:intergenic 40.0%
CCCTCTGGATTATTCTCAAA+TGG - chr2.4:69324244-69324263 None:intergenic 40.0%
CCTTTGAATTTCAGAAGCGA+TGG + chr2.4:69324025-69324044 MS.gene004220:intron 40.0%
CTCAAATTGTCGACCACTTA+TGG - chr2.4:69322641-69322660 None:intergenic 40.0%
GACGGTTTGCATCTAACTAA+CGG + chr2.4:69324660-69324679 MS.gene004220:CDS 40.0%
GAGAACGAAAAGTTTCTCTC+TGG + chr2.4:69323336-69323355 MS.gene004220:intron 40.0%
GGAAAACCAACCTCTACTTT+AGG + chr2.4:69323772-69323791 MS.gene004220:intron 40.0%
GGGTCAATATCAGATACAAG+GGG - chr2.4:69324768-69324787 None:intergenic 40.0%
GGTCAATATCAGATACAAGG+GGG - chr2.4:69324767-69324786 None:intergenic 40.0%
GTACGAGATGCTTGTGTAAA+AGG + chr2.4:69323569-69323588 MS.gene004220:intron 40.0%
GTGATGTTAAATGTGGACTC+TGG + chr2.4:69323468-69323487 MS.gene004220:intron 40.0%
GTTTGCATCTAACTAACGGA+GGG + chr2.4:69324664-69324683 MS.gene004220:CDS 40.0%
TAACATGTAACAGGCAGATG+TGG + chr2.4:69322764-69322783 MS.gene004220:intron 40.0%
TACCTTGAAGAAGGAGACAA+TGG - chr2.4:69322997-69323016 None:intergenic 40.0%
TAGGCATGCATGTTAGCAAT+GGG + chr2.4:69323749-69323768 MS.gene004220:intron 40.0%
TGTCCCTCTACATGAATACA+AGG + chr2.4:69322959-69322978 MS.gene004220:CDS 40.0%
TGTTTACCGAAATGCAGTGA+CGG + chr2.4:69324642-69324661 MS.gene004220:intron 40.0%
TTCAAACTCTGTACGGGTAT+GGG - chr2.4:69323021-69323040 None:intergenic 40.0%
TTCATTGGAATCACGCATTC+GGG + chr2.4:69322509-69322528 MS.gene004220:intron 40.0%
TTCTTCCACTCCTAAAGTAG+AGG - chr2.4:69323785-69323804 None:intergenic 40.0%
TTGGTCCAGAGATCAATGTA+AGG - chr2.4:69324343-69324362 None:intergenic 40.0%
TTTGGTCGCGTATATATTGC+AGG + chr2.4:69323216-69323235 MS.gene004220:intron 40.0%
! AATGCCTTTAAGGGGTCATT+AGG - chr2.4:69324789-69324808 None:intergenic 40.0%
! ATGCCTTTAAGGGGTCATTA+GGG - chr2.4:69324788-69324807 None:intergenic 40.0%
! CTACTTTAGGAGTGGAAGAA+TGG + chr2.4:69323785-69323804 MS.gene004220:intron 40.0%
! TAATGGGTATGCACACAATG+AGG + chr2.4:69323054-69323073 MS.gene004220:intron 40.0%
! TGAAAAGCAGAGCATCTACT+GGG - chr2.4:69322936-69322955 None:intergenic 40.0%
! TTGAAAAGCAGAGCATCTAC+TGG - chr2.4:69322937-69322956 None:intergenic 40.0%
!! CCATCGCTTCTGAAATTCAA+AGG - chr2.4:69324028-69324047 None:intergenic 40.0%
!! TTTTGGTGACTCAATCACTG+AGG + chr2.4:69322202-69322221 MS.gene004220:CDS 40.0%
AAATGCAACAGTTCCCTGAC+TGG + chr2.4:69324362-69324381 MS.gene004220:CDS 45.0%
AATACCTGAATGCGAAGAGC+CGG + chr2.4:69322613-69322632 MS.gene004220:intron 45.0%
ACGGCTATAGTGTAGGACTA+GGG + chr2.4:69323392-69323411 MS.gene004220:intron 45.0%
AGAAGGAGACAATGGAGCAA+AGG - chr2.4:69322989-69323008 None:intergenic 45.0%
AGGCATGCATGTTAGCAATG+GGG + chr2.4:69323750-69323769 MS.gene004220:intron 45.0%
CAAAGCAATCGGAGCAGTTT+CGG - chr2.4:69322880-69322899 None:intergenic 45.0%
CCAAATTCCTGAAATCCCAC+AGG - chr2.4:69323433-69323452 None:intergenic 45.0%
CCTGTGGGATTTCAGGAATT+TGG + chr2.4:69323430-69323449 MS.gene004220:intron 45.0%
CTCCATTGTCTCCTTCTTCA+AGG + chr2.4:69322992-69323011 MS.gene004220:CDS 45.0%
GAAAGGACTAATGAAGCTGC+TGG + chr2.4:69324271-69324290 MS.gene004220:CDS 45.0%
GAGGAATCCTTTGATGTTGG+AGG + chr2.4:69322221-69322240 MS.gene004220:CDS 45.0%
GAGTCGTGCAACTTCATCAA+TGG - chr2.4:69324085-69324104 None:intergenic 45.0%
GCAGCTTCATTAGTCCTTTC+AGG - chr2.4:69324271-69324290 None:intergenic 45.0%
GCGTGATTCCAATGAATGCA+TGG - chr2.4:69322505-69322524 None:intergenic 45.0%
GGTTTGCATCTAACTAACGG+AGG + chr2.4:69324663-69324682 MS.gene004220:CDS 45.0%
GTATGCACACAATGAGGGAT+TGG + chr2.4:69323060-69323079 MS.gene004220:intron 45.0%
GTGGGATTTCAGGAATTTGG+AGG + chr2.4:69323433-69323452 MS.gene004220:intron 45.0%
TACCGTGCGTGAGAAATAGT+TGG - chr2.4:69322264-69322283 None:intergenic 45.0%
TCCACTCCTAAAGTAGAGGT+TGG - chr2.4:69323781-69323800 None:intergenic 45.0%
TCCATTCCCACAGAAAAGCA+TGG - chr2.4:69323545-69323564 None:intergenic 45.0%
TCCTTTGATGTTGGAGGATG+GGG + chr2.4:69322227-69322246 MS.gene004220:CDS 45.0%
TGACCCTAATGACCCCTTAA+AGG + chr2.4:69324782-69324801 MS.gene004220:CDS 45.0%
TGCAAACCGTCACTGCATTT+CGG - chr2.4:69324651-69324670 None:intergenic 45.0%
TTGATGAAGTTGCACGACTC+AGG + chr2.4:69324085-69324104 MS.gene004220:CDS 45.0%
TTGTCGACCACTTATGGATC+CGG - chr2.4:69322635-69322654 None:intergenic 45.0%
TTGTTCTTTAGTGGCACAGC+AGG + chr2.4:69323133-69323152 MS.gene004220:intron 45.0%
! ACCAACCTCTACTTTAGGAG+TGG + chr2.4:69323777-69323796 MS.gene004220:intron 45.0%
! AGAGTTTTCCCTGTGTCACA+AGG + chr2.4:69322840-69322859 MS.gene004220:CDS 45.0%
! AGGGATTGGCACAGATTATC+AGG + chr2.4:69323074-69323093 MS.gene004220:intron 45.0%
! CTTTTCTGTGGGAATGGAGT+TGG + chr2.4:69323547-69323566 MS.gene004220:intron 45.0%
! GGTATGGGTTACCTTGAAGA+AGG - chr2.4:69323006-69323025 None:intergenic 45.0%
! GTCAGGGAACTGTTGCATTT+TGG - chr2.4:69324362-69324381 None:intergenic 45.0%
! TCCATGCTTTTCTGTGGGAA+TGG + chr2.4:69323541-69323560 MS.gene004220:intron 45.0%
! TGCCAACTATTTCTCACGCA+CGG + chr2.4:69322259-69322278 MS.gene004220:CDS 45.0%
! TGGTGCTAAGAGGGTATAGT+GGG + chr2.4:69322784-69322803 MS.gene004220:CDS 45.0%
!! TCTTCGCATTCAGGTATTGG+TGG - chr2.4:69322611-69322630 None:intergenic 45.0%
!! ATAAAAAAAATAAAAAAAAG+TGG - chr2.4:69323596-69323615 None:intergenic 5.0%
!!! AATAATAATTATTTTTCATT+TGG + chr2.4:69323246-69323265 MS.gene004220:intron 5.0%
ACAGTGCACGGCTATAGTGT+AGG + chr2.4:69323385-69323404 MS.gene004220:intron 50.0%
ACCCCATCCTCCAACATCAA+AGG - chr2.4:69322231-69322250 None:intergenic 50.0%
ATCCAGAGGGTCTTCCTGAA+AGG + chr2.4:69324254-69324273 MS.gene004220:CDS 50.0%
CAATGGGAGGAGGAGTTATG+AGG - chr2.4:69324068-69324087 None:intergenic 50.0%
CACGGCTATAGTGTAGGACT+AGG + chr2.4:69323391-69323410 MS.gene004220:intron 50.0%
CGTGCAACTTCATCAATGGG+AGG - chr2.4:69324081-69324100 None:intergenic 50.0%
GATACAAGGGGGAGATCAAC+TGG - chr2.4:69324756-69324775 None:intergenic 50.0%
GCAACTTCATCAATGGGAGG+AGG - chr2.4:69324078-69324097 None:intergenic 50.0%
GGCATGCATGTTAGCAATGG+GGG + chr2.4:69323751-69323770 MS.gene004220:intron 50.0%
GGCTCTTCGCATTCAGGTAT+TGG - chr2.4:69322614-69322633 None:intergenic 50.0%
TAAAGCTGTGGCACAGTGCA+CGG + chr2.4:69323373-69323392 MS.gene004220:intron 50.0%
TGCTAGAGCATGCATTGCTG+TGG + chr2.4:69324300-69324319 MS.gene004220:CDS 50.0%
TTCTTTAGTGGCACAGCAGG+AGG + chr2.4:69323136-69323155 MS.gene004220:intron 50.0%
TTTACACTTGCGTGGCCCAT+AGG + chr2.4:69323865-69323884 MS.gene004220:intron 50.0%
! AGGCAGATGTGGTGCTAAGA+GGG + chr2.4:69322775-69322794 MS.gene004220:intron 50.0%
! GTGGTGCTAAGAGGGTATAG+TGG + chr2.4:69322783-69322802 MS.gene004220:CDS 50.0%
! GTTTTCCCTGTGTCACAAGG+CGG + chr2.4:69322843-69322862 MS.gene004220:CDS 50.0%
! TAGTGGGTACAACACGAGGT+GGG + chr2.4:69322800-69322819 MS.gene004220:CDS 50.0%
!! ATAGTGGGTACAACACGAGG+TGG + chr2.4:69322799-69322818 MS.gene004220:CDS 50.0%
!! GGTATAGTGGGTACAACACG+AGG + chr2.4:69322796-69322815 MS.gene004220:CDS 50.0%
ACATGGCCCTGTGGGATTTC+AGG + chr2.4:69323423-69323442 MS.gene004220:intron 55.0%
CGAAGAGCCGGATCCATAAG+TGG + chr2.4:69322625-69322644 MS.gene004220:intron 55.0%
GTCCTTTCAGGAAGACCCTC+TGG - chr2.4:69324259-69324278 None:intergenic 55.0%
TGTCACCGCCTTGTGACACA+GGG - chr2.4:69322851-69322870 None:intergenic 55.0%
! CAGGCAGATGTGGTGCTAAG+AGG + chr2.4:69322774-69322793 MS.gene004220:intron 55.0%
ATGCACGCACATGGCCCTGT+GGG + chr2.4:69323415-69323434 MS.gene004220:intron 60.0%
CTGTCACCGCCTTGTGACAC+AGG - chr2.4:69322852-69322871 None:intergenic 60.0%
GGACTAGGGATGCACGCACA+TGG + chr2.4:69323406-69323425 MS.gene004220:intron 60.0%
GGATCCGGCTCTTCGCATTC+AGG - chr2.4:69322620-69322639 None:intergenic 60.0%
GTGTCACAAGGCGGTGACAG+TGG + chr2.4:69322852-69322871 MS.gene004220:CDS 60.0%
!! CAACACGAGGTGGGCACTGA+AGG + chr2.4:69322809-69322828 MS.gene004220:CDS 60.0%
!! GAGGTGGGCACTGAAGGTGT+TGG + chr2.4:69322815-69322834 MS.gene004220:CDS 60.0%
GATGCACGCACATGGCCCTG+TGG + chr2.4:69323414-69323433 MS.gene004220:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 69322176 69324815 69322176 ID=MS.gene004220
chr2.4 mRNA 69322176 69324815 69322176 ID=MS.gene004220.t1;Parent=MS.gene004220
chr2.4 exon 69322176 69322280 69322176 ID=MS.gene004220.t1.exon1;Parent=MS.gene004220.t1
chr2.4 CDS 69322176 69322280 69322176 ID=cds.MS.gene004220.t1;Parent=MS.gene004220.t1
chr2.4 exon 69322777 69323013 69322777 ID=MS.gene004220.t1.exon2;Parent=MS.gene004220.t1
chr2.4 CDS 69322777 69323013 69322777 ID=cds.MS.gene004220.t1;Parent=MS.gene004220.t1
chr2.4 exon 69324039 69324106 69324039 ID=MS.gene004220.t1.exon3;Parent=MS.gene004220.t1
chr2.4 CDS 69324039 69324106 69324039 ID=cds.MS.gene004220.t1;Parent=MS.gene004220.t1
chr2.4 exon 69324237 69324401 69324237 ID=MS.gene004220.t1.exon4;Parent=MS.gene004220.t1
chr2.4 CDS 69324237 69324401 69324237 ID=cds.MS.gene004220.t1;Parent=MS.gene004220.t1
chr2.4 exon 69324659 69324815 69324659 ID=MS.gene004220.t1.exon5;Parent=MS.gene004220.t1
chr2.4 CDS 69324659 69324815 69324659 ID=cds.MS.gene004220.t1;Parent=MS.gene004220.t1
Gene Sequence

>MS.gene004220

ATGACCAAGAGGCCACAAATATATCTTTTTGGTGACTCAATCACTGAGGAATCCTTTGATGTTGGAGGATGGGGTGCTTCTCTTGCCAACTATTTCTCACGCACGGTAACATCCATTCTTGCTTTGTTTTGCTTTAACAACTCATATTTTCATATCTGATTCATGTTCTTGTTAACATTCCACATTATATGTTTTGTTTATAAATGATTTATTTATTAGTCTCATCGTGTTAGGTGCAACCCAAATTGTAATATGATTTATTTATTAGTCTCGTCGTGTTAAGAATCTCACGTTGACATTGAGTTTATAAGTGGTGAAACTGGATTCCATGCATTCATTGGAATCACGCATTCGGGACATTGTTGATCGTTCTAAATTTTTTATTTCAAAATATAGAAATTGAATCTAAAGTGTCTGATCTTGATCAAACGGCCACCAATACCTGAATGCGAAGAGCCGGATCCATAAGTGGTCGACAATTTGAGTTAATTGTATCCCACATTCTGTTTTTAGATTTTAATGTAAAGTTTGAATCTTTTTGTAATGGGGTTTGTGTTTCTTTTGAAAAGGATAAATGAGGTTTGTTTGTAACATGTAACAGGCAGATGTGGTGCTAAGAGGGTATAGTGGGTACAACACGAGGTGGGCACTGAAGGTGTTGGACAGAGTTTTCCCTGTGTCACAAGGCGGTGACAGTGGAACCGAAACTGCTCCGATTGCTTTGACTGTTTTCTTTGGGGCTAATGACGCTTGTCTTCCCAGTAGATGCTCTGCTTTTCAACATGTCCCTCTACATGAATACAAGGAGAACCTTTGCTCCATTGTCTCCTTCTTCAAGGTAACCCATACCCGTACAGAGTTTGAATAAAATTAGAGTTTAATGGGTATGCACACAATGAGGGATTGGCACAGATTATCAGGTAAAAGACTACTGTGTTGAAACCCAATAAAGGATCATTGTTCTTTAGTGGCACAGCAGGAGGCTTTTTTCTTGAAAAAAAAAAAATAGTTTTGATCTCTTGTGGATTTGATAAGTGTGTTTTGGTCGCGTATATATTGCAGGTTACAGAAATAATAATTATTTTTCATTTGGAAGTTTGCCGCTGTTATGATTATTTAAGTATAGTCTTGCACATTCTAATAATGTTGCATTAGATCTAGAGAACGAAAAGTTTCTCTCTGGATTTATAATGTTTGTAAAGCTGTGGCACAGTGCACGGCTATAGTGTAGGACTAGGGATGCACGCACATGGCCCTGTGGGATTTCAGGAATTTGGAGGTTAATACAATAGGTGATGTTAAATGTGGACTCTGGCTTTTTGCCACTACTGTATGATCAATAATACACACTCCAAAGAGTTTGATTCCATGCTTTTCTGTGGGAATGGAGTTGGTACGAGATGCTTGTGTAAAAGGTCCACTTTTTTTTATTTTTTTTATTGAATTGAGTCTAGCATTGTTGTTAGCGTTGTTTTGACAAACCTAAACATCATGCCTTCTTTCTTTGTGCCATTACTTGGTTCCTGTGCAATTATGTTATCAGTTGGTTTGGTCTCATGTGAGCTGTTAAGTATAGGCATGCATGTTAGCAATGGGGGAAAACCAACCTCTACTTTAGGAGTGGAAGAATGGTCCCTTTCTTTAAGGATCAAAGATATCTGCTTATAACATTCTTAAAGGAAAGTTTTTTTTACACTTGCGTGGCCCATAGGTTGATCAATGTTCATGCTTATATTAAGAAAGTTGTTATCCTTAAATGTATATGTAGGTATATAAGTTGACGCTTGAATATTTTTAATTTCAATTCTAGGCCTCTTGTATAAATGGTATAATTAGACACTGCAAATTTCCTTTGAATTTCAGAAGCGATGGCCGACAAGTAAAGTCATCCTCATAACTCCTCCTCCCATTGATGAAGTTGCACGACTCAGGTATTGTTTTATTGGCTTTTTATCTTCATTTTGTCACTGATTTTATTATGTTTTCTTTGTTTGTGACATTTAAACATATAATTCTATAGAGGTTAATCACACTTGAAGAATTTCTAAAAATCTATGACAGATATCCATTTGAGAATAATCCAGAGGGTCTTCCTGAAAGGACTAATGAAGCTGCTGGTGAATATGCTAGAGCATGCATTGCTGTGGCCACTGAATGTCATATTCCTTACATTGATCTCTGGACCAAAATGCAACAGTTCCCTGACTGGAAAAAAGTTTATTTAAGGTTTATAATTTATTTTACCTTCTTTGTTTATCTTGGCTTACAACATTTTGCATTTTTGCATCATACATATAAAAGCTTACGAAGTTTTCATTGCTGACTTGATTGATTTTTCAAAATGATCAATTCTGTAGCCATATATGTGACTTGAGTTATGCGAAAATGACTAATTCTGTATGAATTTGCAAAGAATAATATCATTATTTAATGCATATGGTATGATTTGATATGAATATACGGTTTTGTTTACCGAAATGCAGTGACGGTTTGCATCTAACTAACGGAGGGAATCAACTTGTATTTGAAGAAGTAATAAAAAAGCTGAGAGATGAAGGTTTGAGTCTAGAATCTATTCCAGTTGATCTCCCCCTTGTATCTGATATTGACCCTAATGACCCCTTAAAGGCATTTCTGTAG
Protein sequence

>MS.gene004220.t1

MTKRPQIYLFGDSITEESFDVGGWGASLANYFSRTADVVLRGYSGYNTRWALKVLDRVFPVSQGGDSGTETAPIALTVFFGANDACLPSRCSAFQHVPLHEYKENLCSIVSFFKKRWPTSKVILITPPPIDEVARLRYPFENNPEGLPERTNEAAGEYARACIAVATECHIPYIDLWTKMQQFPDWKKVYLSDGLHLTNGGNQLVFEEVIKKLRDEGLSLESIPVDLPLVSDIDPNDPLKAFL