AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004377

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004377.t1 MTR_2g018750 98.191 608 10 1 1 608 1 607 0.0 1233
MS.gene004377.t1 MTR_1g064360 70.289 589 140 7 1 581 1 562 0.0 812
MS.gene004377.t1 MTR_1g094850 53.445 595 265 6 1 589 1 589 0.0 640
MS.gene004377.t1 MTR_2g048390 57.582 455 182 7 1 447 1 452 0.0 524
MS.gene004377.t1 MTR_8g078900 38.839 672 331 20 4 606 1 661 1.14e-147 442
MS.gene004377.t1 MTR_2g084595 37.161 627 348 10 9 601 6 620 1.87e-140 422
MS.gene004377.t1 MTR_2g009340 38.684 623 322 12 1 569 19 635 5.55e-139 420
MS.gene004377.t1 MTR_8g078900 38.050 636 321 18 37 607 1 628 4.46e-135 408
MS.gene004377.t1 MTR_4g093850 39.187 615 318 13 1 567 19 625 2.48e-134 408
MS.gene004377.t1 MTR_8g078900 38.898 581 300 15 74 607 46 618 3.54e-131 398
MS.gene004377.t1 MTR_4g022330 36.581 585 322 12 14 576 23 580 2.32e-117 362
MS.gene004377.t1 MTR_1g014070 40.557 503 277 10 1 497 1 487 1.34e-116 360
MS.gene004377.t1 MTR_7g073980 39.083 545 284 13 26 550 33 549 8.14e-116 359
MS.gene004377.t1 MTR_5g016060 36.000 550 297 9 20 561 34 536 2.57e-115 357
MS.gene004377.t1 MTR_5g032780 40.113 531 281 9 1 502 1 523 8.37e-115 356
MS.gene004377.t1 MTR_8g078900 41.139 474 238 13 4 447 1 463 2.72e-114 349
MS.gene004377.t1 MTR_5g015790 40.557 503 274 12 1 498 1 483 6.71e-111 346
MS.gene004377.t1 MTR_1g106430 35.906 596 340 14 1 587 1 563 8.08e-110 343
MS.gene004377.t1 MTR_3g062540 35.765 562 277 10 12 561 11 500 4.84e-109 339
MS.gene004377.t1 MTR_2g084595 38.053 452 255 5 9 447 6 445 3.58e-108 333
MS.gene004377.t1 MTR_4g093850 39.666 479 250 9 1 447 19 490 1.18e-105 329
MS.gene004377.t1 MTR_8g090220 33.710 620 327 14 26 605 52 627 5.48e-105 331
MS.gene004377.t1 MTR_4g095430 36.382 492 281 9 20 505 7 472 7.48e-104 327
MS.gene004377.t1 MTR_8g090220 37.177 503 245 11 26 499 52 512 2.55e-101 317
MS.gene004377.t1 MTR_5g075640 35.119 504 279 7 12 505 12 477 3.49e-101 319
MS.gene004377.t1 MTR_1g088885 34.053 602 342 13 2 575 13 587 5.45e-99 315
MS.gene004377.t1 MTR_4g134210 35.141 498 296 6 1 496 1 473 5.26e-98 311
MS.gene004377.t1 MTR_8g078900 42.228 386 203 9 74 447 46 423 7.94e-98 305
MS.gene004377.t1 MTR_7g108290 35.050 602 337 15 9 578 20 599 1.49e-97 312
MS.gene004377.t1 MTR_3g062540 34.659 528 262 9 46 561 2 458 3.56e-96 304
MS.gene004377.t1 MTR_8g069330 36.697 545 269 13 1 514 1 500 3.26e-95 304
MS.gene004377.t1 MTR_5g075640 34.468 470 261 6 46 505 2 434 2.19e-89 287
MS.gene004377.t1 MTR_2g027410 34.661 502 283 9 1 496 1 463 3.82e-88 285
MS.gene004377.t1 MTR_1g082690 30.417 503 285 7 11 505 43 488 3.12e-83 271
MS.gene004377.t1 MTR_5g016060 34.120 466 254 8 104 561 2 422 1.36e-82 268
MS.gene004377.t1 MTR_2g027410 34.192 427 240 7 75 496 1 391 6.99e-71 237
MS.gene004377.t1 MTR_4g011530 32.812 384 226 7 206 576 38 402 1.14e-63 216
MS.gene004377.t1 MTR_2g102730 33.894 357 211 4 214 563 24 362 1.20e-59 205
MS.gene004377.t1 MTR_4g045767 27.574 573 333 17 35 573 14 538 1.73e-55 197
MS.gene004377.t1 MTR_4g074190 27.126 494 311 12 29 502 6 470 2.16e-49 180
MS.gene004377.t1 MTR_1g035720 25.521 576 328 17 29 561 6 523 8.32e-49 178
MS.gene004377.t1 MTR_4g074190 27.716 451 279 10 70 502 3 424 2.02e-48 177
MS.gene004377.t1 MTR_4g074190 25.624 441 279 12 29 449 6 417 3.03e-37 144
MS.gene004377.t1 MTR_2g048375 50.382 131 52 2 464 581 1 131 2.01e-32 123
MS.gene004377.t1 MTR_1g105870 33.663 202 115 4 33 233 38 221 1.25e-30 121
MS.gene004377.t1 MTR_2g010890 26.195 481 289 15 94 561 1 428 1.73e-27 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004377.t1 AT5G03250 64.047 598 193 8 1 588 1 586 0.0 783
MS.gene004377.t1 AT1G30440 49.530 638 280 10 1 597 1 637 0.0 619
MS.gene004377.t1 AT5G13600 55.482 602 222 13 1 584 1 574 0.0 613
MS.gene004377.t1 AT5G03250 61.728 486 164 8 113 588 1 474 0.0 605
MS.gene004377.t1 AT5G66560 39.219 691 299 18 1 596 1 665 3.20e-151 451
MS.gene004377.t1 AT3G44820 38.485 647 326 13 8 602 5 631 1.18e-147 441
MS.gene004377.t1 AT5G66560 38.321 655 290 17 37 596 1 636 1.75e-138 417
MS.gene004377.t1 AT5G66560 40.517 580 273 16 70 596 66 626 4.26e-135 408
MS.gene004377.t1 AT3G50840 38.833 600 304 16 18 596 7 564 9.32e-128 387
MS.gene004377.t1 AT5G48800 38.255 596 287 12 14 576 30 577 5.41e-120 369
MS.gene004377.t1 AT3G50840 37.368 570 295 15 47 596 1 528 3.97e-113 348
MS.gene004377.t1 AT3G50840 37.368 570 295 15 47 596 1 528 3.97e-113 348
MS.gene004377.t1 AT5G67385 38.105 496 277 9 12 504 12 480 6.43e-113 350
MS.gene004377.t1 AT5G66560 40.200 500 225 12 1 447 1 479 7.83e-111 341
MS.gene004377.t1 AT3G08570 37.662 539 302 10 26 550 34 552 6.76e-108 338
MS.gene004377.t1 AT4G37590 38.012 513 255 12 1 498 1 465 8.99e-107 333
MS.gene004377.t1 AT1G67900 38.815 523 281 8 1 498 1 509 2.19e-106 334
MS.gene004377.t1 AT1G67900 38.815 523 281 8 1 498 1 509 2.19e-106 334
MS.gene004377.t1 AT1G67900 38.815 523 281 8 1 498 1 509 2.19e-106 334
MS.gene004377.t1 AT1G67900 38.815 523 281 8 1 498 1 509 2.19e-106 334
MS.gene004377.t1 AT1G67900 38.815 523 281 8 1 498 1 509 2.19e-106 334
MS.gene004377.t1 AT1G67900 38.815 523 281 8 1 498 1 509 2.19e-106 334
MS.gene004377.t1 AT1G03010 36.441 590 311 14 24 575 7 570 5.61e-106 332
MS.gene004377.t1 AT1G03010 35.961 609 325 15 5 575 16 597 6.25e-106 333
MS.gene004377.t1 AT1G03010 36.181 597 313 15 20 574 25 595 1.76e-105 332
MS.gene004377.t1 AT4G31820 37.698 504 272 11 1 498 1 468 8.36e-104 326
MS.gene004377.t1 AT5G10250 33.447 589 310 12 12 567 36 575 1.93e-102 323
MS.gene004377.t1 AT2G30520 35.968 506 280 10 4 504 9 475 2.96e-102 322
MS.gene004377.t1 AT3G08660 35.273 550 296 10 24 560 32 534 7.79e-102 321
MS.gene004377.t1 AT2G14820 38.039 510 290 13 1 502 1 492 2.91e-101 321
MS.gene004377.t1 AT2G14820 38.039 510 290 13 1 502 1 492 2.91e-101 321
MS.gene004377.t1 AT2G47860 34.385 602 329 14 5 573 17 585 4.38e-101 320
MS.gene004377.t1 AT5G67440 37.573 511 283 12 1 504 1 482 1.21e-99 315
MS.gene004377.t1 AT5G67440 37.573 511 283 12 1 504 1 482 1.21e-99 315
MS.gene004377.t1 AT5G67385 37.093 461 261 8 47 504 1 435 2.90e-99 313
MS.gene004377.t1 AT5G67385 37.093 461 261 8 47 504 1 435 2.90e-99 313
MS.gene004377.t1 AT5G67385 37.093 461 261 8 47 504 1 435 2.90e-99 313
MS.gene004377.t1 AT5G67385 37.093 461 261 8 47 504 1 435 2.90e-99 313
MS.gene004377.t1 AT5G10250 33.333 579 309 12 12 567 36 560 6.46e-98 311
MS.gene004377.t1 AT2G47860 33.120 625 329 15 5 573 17 608 7.26e-96 307
MS.gene004377.t1 AT2G23050 35.673 513 268 11 1 506 1 458 3.42e-95 300
MS.gene004377.t1 AT3G26490 37.072 526 284 14 1 498 1 507 1.65e-92 296
MS.gene004377.t1 AT3G26490 37.072 526 284 14 1 498 1 507 1.65e-92 296
MS.gene004377.t1 AT3G26490 37.072 526 284 14 1 498 1 507 1.65e-92 296
MS.gene004377.t1 AT3G08660 35.375 506 270 9 66 558 11 472 3.52e-92 294
MS.gene004377.t1 AT2G30520 35.345 464 257 9 46 504 2 427 2.51e-89 287
MS.gene004377.t1 AT2G30520 35.345 464 257 9 46 504 2 427 2.51e-89 287
MS.gene004377.t1 AT5G67440 36.809 470 262 11 41 504 5 445 3.07e-88 284
MS.gene004377.t1 AT5G47800 32.738 504 301 9 1 496 1 474 1.03e-86 281
MS.gene004377.t1 AT5G47800 32.738 504 301 9 1 496 1 474 1.03e-86 281
MS.gene004377.t1 AT5G47800 32.738 504 301 9 1 496 1 474 1.03e-86 281
MS.gene004377.t1 AT5G47800 32.738 504 301 9 1 496 114 587 1.89e-85 280
MS.gene004377.t1 AT4G31820 35.698 451 249 10 53 498 1 415 4.91e-84 272
MS.gene004377.t1 AT3G49970 33.778 450 237 9 36 483 4 394 1.08e-80 263
MS.gene004377.t1 AT3G49970 33.778 450 237 9 36 483 4 394 1.08e-80 263
MS.gene004377.t1 AT3G08660 34.873 433 228 9 24 449 32 417 2.27e-74 244
MS.gene004377.t1 AT2G47860 34.565 460 251 12 5 447 17 443 8.05e-73 241
MS.gene004377.t1 AT3G26490 34.664 476 264 14 1 448 1 457 2.28e-69 233
MS.gene004377.t1 AT2G47860 33.126 483 250 13 5 447 17 466 6.45e-68 228
MS.gene004377.t1 AT2G47860 32.186 494 275 12 113 573 1 467 5.55e-67 227
MS.gene004377.t1 AT2G47860 32.186 494 275 12 113 573 1 467 5.55e-67 227
MS.gene004377.t1 AT5G64330 42.045 264 137 3 7 256 33 294 1.88e-63 221
MS.gene004377.t1 AT5G64330 46.091 243 117 1 276 504 369 611 1.40e-58 208
MS.gene004377.t1 AT5G64330 42.045 264 137 3 7 256 33 294 7.09e-63 221
MS.gene004377.t1 AT5G64330 46.091 243 117 1 276 504 369 611 2.10e-58 209
MS.gene004377.t1 AT2G23050 33.501 397 206 9 113 506 1 342 1.90e-59 203
MS.gene004377.t1 AT5G64330 46.091 243 117 1 276 504 310 552 5.29e-59 208
MS.gene004377.t1 AT5G64330 42.241 232 118 3 39 256 6 235 2.02e-51 187
MS.gene004377.t1 AT1G52770 33.842 393 210 7 214 580 28 396 1.55e-58 203
MS.gene004377.t1 AT3G19850 28.631 482 293 10 36 497 14 464 9.71e-55 195
MS.gene004377.t1 AT5G17580 29.918 488 285 10 28 504 22 463 1.54e-54 195
MS.gene004377.t1 AT3G15570 33.766 385 211 9 209 567 21 387 1.60e-54 192
MS.gene004377.t1 AT5G17580 29.918 488 285 10 28 504 7 448 1.80e-54 194
MS.gene004377.t1 AT3G03510 29.354 511 258 15 82 576 8 431 3.56e-53 189
MS.gene004377.t1 AT3G22104 29.299 471 270 11 22 483 4 420 4.13e-52 187
MS.gene004377.t1 AT1G50280 28.571 476 294 10 35 492 13 460 1.33e-47 175
MS.gene004377.t1 AT5G48130 26.761 497 286 12 19 497 32 468 2.82e-47 176
MS.gene004377.t1 AT1G50280 28.146 437 273 8 70 492 18 427 1.26e-45 169
MS.gene004377.t1 AT3G22104 28.316 392 223 8 100 483 4 345 1.62e-36 142
MS.gene004377.t1 AT3G22104 28.316 392 223 8 100 483 4 345 1.62e-36 142
MS.gene004377.t1 AT3G22104 28.316 392 223 8 100 483 4 345 1.62e-36 142
MS.gene004377.t1 AT3G49900 35.220 159 80 7 20 167 59 205 1.90e-11 67.4
MS.gene004377.t1 AT3G49900 35.220 159 80 7 20 167 59 205 2.12e-11 67.0

Find 124 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCTTCTCATTCCGAATA+TGG 0.185813 2.4:-67530814 MS.gene004377:CDS
TCTAAACCTGTCTAAATTTC+AGG 0.215961 2.4:-67530583 MS.gene004377:CDS
TCTCCATGTATAACTGAAAT+TGG 0.237203 2.4:-67530699 MS.gene004377:CDS
AGATTGTATTCAGAGGATTT+TGG 0.237388 2.4:-67530763 MS.gene004377:CDS
GCACGTTTCAAGCGCTTTGA+TGG 0.261050 2.4:+67531406 None:intergenic
TGTTATGAGCTCAAATGTTT+TGG 0.294973 2.4:+67531589 None:intergenic
AACTCCCTCAACTACTTTCA+TGG 0.296766 2.4:+67529457 None:intergenic
TATGGTGCAGATAATCTTAT+TGG 0.306224 2.4:-67531476 MS.gene004377:CDS
TTTGGATTTAGAAGAAAATC+AGG 0.306365 2.4:-67529546 MS.gene004377:CDS
TTCTAGATGCATTGATTCCT+TGG 0.328608 2.4:-67531345 MS.gene004377:CDS
TTCCTTCCAATGATAGCTTT+TGG 0.329711 2.4:+67529871 None:intergenic
CACAGCCTGTTGGTGATAAT+TGG 0.336858 2.4:-67531208 MS.gene004377:CDS
CCTCAACTACTTTCATGGTT+TGG 0.344002 2.4:+67529462 None:intergenic
CCCTCACGACGAAATGCTTC+AGG 0.350085 2.4:+67532617 None:intergenic
CGGATCCAGATTTGTTCAAT+TGG 0.351033 2.4:-67531307 MS.gene004377:CDS
ACTTGAACAATTACTCTTAG+AGG 0.351406 2.4:+67529819 None:intergenic
TTTACATTATTTGATTCATT+AGG 0.353297 2.4:+67529513 None:intergenic
CAATGCATCTAGAAACAATA+TGG 0.362633 2.4:+67531354 None:intergenic
AGGCTTTGATCCCAGCTTCA+TGG 0.366193 2.4:+67532637 None:intergenic
GAAATTGGAACATTAATTGC+TGG 0.374348 2.4:-67530684 MS.gene004377:CDS
GATGTGACACCTCTCTTATT+AGG 0.375205 2.4:+67530951 None:intergenic
TTGCAACTTCATGATGTTCC+TGG 0.376919 2.4:-67531617 MS.gene004377:CDS
CTTGGCTATGAAGGCTTGTT+CGG 0.381268 2.4:-67531327 MS.gene004377:CDS
TCAAGCGCTTTGATGGAATC+TGG 0.381679 2.4:+67531413 None:intergenic
ACTTGCCAATTGAACAAATC+TGG 0.382711 2.4:+67531302 None:intergenic
CATTGATTCCTTGGCTATGA+AGG 0.384124 2.4:-67531336 MS.gene004377:CDS
AATTCAAGGGTTTGTACAAC+AGG 0.391467 2.4:-67532397 MS.gene004377:intron
TCTAAATTCTCTTTGCATGA+TGG 0.393396 2.4:+67530876 None:intergenic
GCTGGTAGATAGACATAAAA+TGG 0.403847 2.4:+67530739 None:intergenic
CAAAGAGAATTTAGAGAAAA+GGG 0.406093 2.4:-67530868 MS.gene004377:CDS
TGATCAGCTTCAGAAGTTGT+AGG 0.408703 2.4:+67531008 None:intergenic
GCAAAGAGAATTTAGAGAAA+AGG 0.413793 2.4:-67530869 MS.gene004377:CDS
CGCAATAGATGTATACCTTA+AGG 0.419410 2.4:-67530505 MS.gene004377:intron
TCAAGGGGCCTGGCATAATC+AGG 0.421029 2.4:+67530543 None:intergenic
AGACTTCGCACGTCGATATC+CGG 0.422476 2.4:-67529777 MS.gene004377:CDS
CATCTCTTATATATTACCTT+AGG 0.423118 2.4:-67531091 MS.gene004377:CDS
ATTATTTGATTCATTAGGAT+TGG 0.433862 2.4:+67529518 None:intergenic
CTCCAAATGACTGAAAACTA+TGG 0.440150 2.4:-67531494 MS.gene004377:CDS
TTGCAATTCCTGATTATGCC+AGG 0.447101 2.4:-67530551 MS.gene004377:CDS
TTGTTCAATTGGCAAGTGAC+TGG 0.447348 2.4:-67531296 MS.gene004377:CDS
TGAATCGAAAGGAATGAAAT+CGG 0.448048 2.4:-67531126 MS.gene004377:CDS
AAATGAGTGCTGACCATGTT+TGG 0.451217 2.4:+67532594 None:intergenic
CAACCGAAGGAGATGCTTAG+AGG 0.452313 2.4:+67530929 None:intergenic
AACTTCTGAAGCTGATCAAA+GGG 0.458152 2.4:-67531003 MS.gene004377:CDS
AACCACCAATTATCACCAAC+AGG 0.459595 2.4:+67531203 None:intergenic
GATGACAATGGTCGCTAACT+TGG 0.461208 2.4:-67530643 MS.gene004377:CDS
CACGTAATATCATCGCGGTT+CGG 0.462601 2.4:+67530904 None:intergenic
AAATTGGAACATTAATTGCT+GGG 0.462617 2.4:-67530683 MS.gene004377:CDS
CTCTTATATATTACCTTAGG+AGG 0.464964 2.4:-67531088 MS.gene004377:CDS
ACTTGATTGTCTATTCATCA+AGG 0.466219 2.4:+67531058 None:intergenic
TCGACCGAGTATCCCATATT+CGG 0.470589 2.4:+67530801 None:intergenic
GATCAGCTTCAGAAGTTGTA+GGG 0.473303 2.4:+67531009 None:intergenic
TAACAAAATCAAAGAAAAGT+TGG 0.474587 2.4:-67529587 MS.gene004377:CDS
AGGGCAAATATCGGTCCCTC+AGG 0.476825 2.4:-67529711 MS.gene004377:CDS
TTTCCATTACTTTCTAGAAG+TGG 0.477836 2.4:-67531695 MS.gene004377:CDS
TGTAATGGCATCCATGAAGC+TGG 0.480977 2.4:-67532648 None:intergenic
ACATACATGGCTGACAGATT+CGG 0.484321 2.4:-67529925 MS.gene004377:CDS
GCAGAGTCCACAAGTGAGAA+TGG 0.487724 2.4:-67531653 MS.gene004377:CDS
TTCAGATACAAAGAACCAAC+CGG 0.490239 2.4:+67529758 None:intergenic
AGTGATGTTACTATTGAAGT+TGG 0.491007 2.4:-67532367 MS.gene004377:CDS
GAATTGCCAAAAGCTATCAT+TGG 0.494936 2.4:-67529877 MS.gene004377:CDS
GATCTTCTCATTCCGAATAT+GGG 0.495062 2.4:-67530813 MS.gene004377:CDS
ATGGTCGCTAACTTGGTAGA+TGG 0.495307 2.4:-67530636 MS.gene004377:CDS
CAAGTGCCTGAAATTTAGAC+AGG 0.499556 2.4:+67530577 None:intergenic
GAGGAACATGAGTTGTTGAA+TGG 0.502381 2.4:-67531251 MS.gene004377:CDS
TTGAAAACTCTCAAAATCCA+AGG 0.504738 2.4:-67529731 MS.gene004377:CDS
GTTCCAATTTCAGTTATACA+TGG 0.505634 2.4:+67530696 None:intergenic
CAAAATCCAAGGGCAAATAT+CGG 0.506637 2.4:-67529720 MS.gene004377:CDS
AGCCTGTTGGTGATAATTGG+TGG 0.510548 2.4:-67531205 MS.gene004377:CDS
TATACCGTCGTCAAGGGGCC+TGG 0.511288 2.4:+67530533 None:intergenic
ATGGCTGACAGATTCGGAAC+GGG 0.514241 2.4:-67529919 MS.gene004377:CDS
GAGGGACCGATATTTGCCCT+TGG 0.518333 2.4:+67529714 None:intergenic
ACTTGATCCATTCTCACTTG+TGG 0.523773 2.4:+67531646 None:intergenic
GGGTTTACTAGAAGAAATTG+TGG 0.530266 2.4:-67530982 MS.gene004377:CDS
CAACTTCTGAAGCTGATCAA+AGG 0.539281 2.4:-67531004 MS.gene004377:CDS
CAATTATCACCAACAGGCTG+TGG 0.540795 2.4:+67531209 None:intergenic
CATCCTCTAAGCATCTCCTT+CGG 0.541272 2.4:-67530932 MS.gene004377:CDS
CCAAACCATGAAAGTAGTTG+AGG 0.543035 2.4:-67529462 None:intergenic
TATACATGGAGAAGCAGATA+AGG 0.544758 2.4:+67530710 None:intergenic
CAAACCATGAAAGTAGTTGA+GGG 0.548852 2.4:-67529461 None:intergenic
CATTCCGAATATGGGATACT+CGG 0.549415 2.4:-67530805 MS.gene004377:CDS
AAGAGAACGTCTATTAGAGT+TGG 0.549510 2.4:-67529649 MS.gene004377:CDS
GGAGAAGCAGATAAGGATGC+AGG 0.551751 2.4:+67530717 None:intergenic
ACTAGACCAGGCTTCACTTG+TGG 0.556499 2.4:-67530835 MS.gene004377:CDS
AGTCCACTTCTAGAAAGTAA+TGG 0.557324 2.4:+67531692 None:intergenic
GTGGTATATACCGTCGTCAA+GGG 0.566981 2.4:+67530527 None:intergenic
CAACTTCATGATGTTCCTGG+TGG 0.568004 2.4:-67531614 MS.gene004377:CDS
GAAGATCCACAAGTGAAGCC+TGG 0.572388 2.4:+67530829 None:intergenic
CTTGATTGTCTATTCATCAA+GGG 0.575589 2.4:+67531059 None:intergenic
GTCATGCAGCACAAAATGAA+AGG 0.578796 2.4:-67529845 MS.gene004377:CDS
TCCCTCAGGGAGTTGTCAAG+TGG 0.579187 2.4:-67529697 MS.gene004377:CDS
GAGAATTTAGAGAAAAGGGT+AGG 0.579452 2.4:-67530864 MS.gene004377:CDS
TGCCAAAAGCTATCATTGGA+AGG 0.584984 2.4:-67529873 MS.gene004377:CDS
TTGGATTTAGAAGAAAATCA+GGG 0.588050 2.4:-67529545 MS.gene004377:CDS
GCCTGAAGCATTTCGTCGTG+AGG 0.588579 2.4:-67532618 MS.gene004377:CDS
ACTTCAATAGTAACATCACT+TGG 0.592157 2.4:+67532370 None:intergenic
TCAAGGGAAGAAACCTCCTA+AGG 0.594510 2.4:+67531075 None:intergenic
GGTAGGAGCTCAACTAGACC+AGG 0.597267 2.4:-67530847 MS.gene004377:CDS
AAGCAGATAAGGATGCAGGC+TGG 0.598744 2.4:+67530721 None:intergenic
ATGATATAGATTGTATTCAG+AGG 0.601793 2.4:-67530770 MS.gene004377:CDS
GTAATGGCATCCATGAAGCT+GGG 0.602173 2.4:-67532647 MS.gene004377:CDS
TGAAAACTCTCAAAATCCAA+GGG 0.602387 2.4:-67529730 MS.gene004377:CDS
TCCACTTGACAACTCCCTGA+GGG 0.607481 2.4:+67529696 None:intergenic
TCGCGGTTCGGAGCAACCGA+AGG 0.609645 2.4:+67530916 None:intergenic
CCTGAAGCATTTCGTCGTGA+GGG 0.613504 2.4:-67532617 MS.gene004377:CDS
TACGTTGACACCGATGACAA+TGG 0.615680 2.4:-67530655 MS.gene004377:CDS
CGTGGTATATACCGTCGTCA+AGG 0.618223 2.4:+67530526 None:intergenic
CGAACAAGCCTTCATAGCCA+AGG 0.624649 2.4:+67531328 None:intergenic
TTCGTCGTGAGGGCCAAACA+TGG 0.625414 2.4:-67532607 MS.gene004377:intron
TTCGCACGTCGATATCCGGT+TGG 0.629496 2.4:-67529773 MS.gene004377:CDS
CATGGCTGACAGATTCGGAA+CGG 0.631652 2.4:-67529920 MS.gene004377:CDS
AAGTTAGCGACCATTGTCAT+CGG 0.635927 2.4:+67530645 None:intergenic
TGGTATATACCGTCGTCAAG+GGG 0.643375 2.4:+67530528 None:intergenic
GGGCAAATATCGGTCCCTCA+GGG 0.648762 2.4:-67529710 MS.gene004377:CDS
TATGCCAGGCCCCTTGACGA+CGG 0.651135 2.4:-67530537 MS.gene004377:CDS
GAGTTGATTCCTAATAAGAG+AGG 0.668136 2.4:-67530960 MS.gene004377:CDS
AATACAAAAGTACCTTGTGG+AGG 0.673725 2.4:+67532329 None:intergenic
AATTGGAACATTAATTGCTG+GGG 0.676903 2.4:-67530682 MS.gene004377:CDS
ATCCACTTGACAACTCCCTG+AGG 0.676982 2.4:+67529695 None:intergenic
TAAGGTATACATCTATTGCG+TGG 0.691492 2.4:+67530508 None:intergenic
ACTTCTGAAGCTGATCAAAG+GGG 0.700182 2.4:-67531002 MS.gene004377:CDS
CAAAGCGCTTGAAACGTGCG+AGG 0.701306 2.4:-67531402 MS.gene004377:CDS
GAAAGTAAACCACAGCCTGT+TGG 0.711849 2.4:-67531218 MS.gene004377:CDS
ACTTGCACGTAATATCATCG+CGG 0.804434 2.4:+67530899 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATCAAATGAAATTTA+AGG + chr2.4:67529639-67529658 None:intergenic 10.0%
!! AAAAATTTAATTATAAATGC+TGG + chr2.4:67530304-67530323 None:intergenic 10.0%
!!! AAAATCAATCTTTTATATAA+TGG + chr2.4:67530956-67530975 None:intergenic 10.0%
!!! TAAATTTCATTTGATTTTTT+TGG - chr2.4:67529639-67529658 MS.gene004377:CDS 10.0%
!! AGTTCATATAAAAAAATTAC+TGG + chr2.4:67531656-67531675 None:intergenic 15.0%
!! CACATATTATATAAGATATA+AGG + chr2.4:67529558-67529577 None:intergenic 15.0%
!! TTTACATTATTTGATTCATT+AGG + chr2.4:67532598-67532617 None:intergenic 15.0%
!!! AATTAAATTTTTGTCTTTGT+TGG - chr2.4:67530312-67530331 MS.gene004377:intron 15.0%
!!! TAATCTGTGATTTTTATTTA+GGG + chr2.4:67531812-67531831 None:intergenic 15.0%
!!! TTAATCTGTGATTTTTATTT+AGG + chr2.4:67531813-67531832 None:intergenic 15.0%
!! AAAATGTAACAAAAAACTTC+AGG - chr2.4:67532159-67532178 MS.gene004377:intron 20.0%
!! ATGGTTATAATAAGAAAAAG+AGG - chr2.4:67532015-67532034 MS.gene004377:intron 20.0%
!! ATTATTTGATTCATTAGGAT+TGG + chr2.4:67532593-67532612 None:intergenic 20.0%
!! GAACTTTAGTACTATATATA+CGG + chr2.4:67530040-67530059 None:intergenic 20.0%
!! TAACAAAATCAAAGAAAAGT+TGG - chr2.4:67532521-67532540 MS.gene004377:intron 20.0%
!!! ATAAACTAAGAAAGTATGTT+TGG - chr2.4:67532045-67532064 MS.gene004377:intron 20.0%
!!! ATGATCTTGTTTTAATTCAA+GGG - chr2.4:67529698-67529717 MS.gene004377:CDS 20.0%
!!! TGTTACATTTTGAAATGATT+AGG + chr2.4:67532150-67532169 None:intergenic 20.0%
!!! TTGTTCTAATAATTAACTTG+AGG + chr2.4:67529869-67529888 None:intergenic 20.0%
! AAAAAATTACTGGCAAAGAA+AGG + chr2.4:67531646-67531665 None:intergenic 25.0%
! AAATTGGAACATTAATTGCT+GGG - chr2.4:67531425-67531444 MS.gene004377:CDS 25.0%
! AGAAACATCTATACATAGAA+GGG - chr2.4:67531941-67531960 MS.gene004377:intron 25.0%
! ATGATATAGATTGTATTCAG+AGG - chr2.4:67531338-67531357 MS.gene004377:CDS 25.0%
! CAAAGAGAATTTAGAGAAAA+GGG - chr2.4:67531240-67531259 MS.gene004377:CDS 25.0%
! CATCTCTTATATATTACCTT+AGG - chr2.4:67531017-67531036 MS.gene004377:CDS 25.0%
! CTTGAATTAAAACAAGATCA+TGG + chr2.4:67529699-67529718 None:intergenic 25.0%
! TAGAAACATCTATACATAGA+AGG - chr2.4:67531940-67531959 MS.gene004377:intron 25.0%
! TATGTACAAATTGCAAAACA+TGG - chr2.4:67531996-67532015 MS.gene004377:intron 25.0%
! TCATATATAGACACATAGTA+GGG + chr2.4:67530130-67530149 None:intergenic 25.0%
! TTGGATTTAGAAGAAAATCA+GGG - chr2.4:67532563-67532582 MS.gene004377:intron 25.0%
! TTTGGATTTAGAAGAAAATC+AGG - chr2.4:67532562-67532581 MS.gene004377:intron 25.0%
!! AGCATTTTTCCAAAAAGATT+TGG - chr2.4:67532544-67532563 MS.gene004377:intron 25.0%
!! CTTCTAAATCCAAATCTTTT+TGG + chr2.4:67532556-67532575 None:intergenic 25.0%
!! TAAATGAAGTTTTCAGCAAT+TGG - chr2.4:67530672-67530691 MS.gene004377:CDS 25.0%
!! TCAATCTTTTATATAATGGC+AGG + chr2.4:67530952-67530971 None:intergenic 25.0%
!!! ATTTTAGCTATTGAATCGAA+AGG - chr2.4:67530971-67530990 MS.gene004377:CDS 25.0%
!!! CATGATCTTGTTTTAATTCA+AGG - chr2.4:67529697-67529716 MS.gene004377:CDS 25.0%
!!! TTAAATACAAAAGTACCTTG+TGG + chr2.4:67529785-67529804 None:intergenic 25.0%
!!! TTAAGGTTCAATTTTGACAT+AGG + chr2.4:67529622-67529641 None:intergenic 25.0%
!!! TTTTTTTGGATAGTGAGAAT+AGG - chr2.4:67529653-67529672 MS.gene004377:CDS 25.0%
AATTGGAACATTAATTGCTG+GGG - chr2.4:67531426-67531445 MS.gene004377:CDS 30.0%
ACTTCAATAGTAACATCACT+TGG + chr2.4:67529741-67529760 None:intergenic 30.0%
ACTTGAACAATTACTCTTAG+AGG + chr2.4:67532292-67532311 None:intergenic 30.0%
ACTTGATTGTCTATTCATCA+AGG + chr2.4:67531053-67531072 None:intergenic 30.0%
AGTACATGATTGTGATTTCA+TGG + chr2.4:67532097-67532116 None:intergenic 30.0%
AGTGATGTTACTATTGAAGT+TGG - chr2.4:67529741-67529760 MS.gene004377:CDS 30.0%
CAATGCATCTAGAAACAATA+TGG + chr2.4:67530757-67530776 None:intergenic 30.0%
CATATATAGACACATAGTAG+GGG + chr2.4:67530129-67530148 None:intergenic 30.0%
CATGGTACTAAATTTGTGAA+AGG + chr2.4:67532079-67532098 None:intergenic 30.0%
CTCATATATAGACACATAGT+AGG + chr2.4:67530131-67530150 None:intergenic 30.0%
CTCTTATATATTACCTTAGG+AGG - chr2.4:67531020-67531039 MS.gene004377:CDS 30.0%
CTTGATTGTCTATTCATCAA+GGG + chr2.4:67531052-67531071 None:intergenic 30.0%
GAAATTGGAACATTAATTGC+TGG - chr2.4:67531424-67531443 MS.gene004377:CDS 30.0%
GCAAAGAGAATTTAGAGAAA+AGG - chr2.4:67531239-67531258 MS.gene004377:CDS 30.0%
GTTCCAATTTCAGTTATACA+TGG + chr2.4:67531415-67531434 None:intergenic 30.0%
TAATGATGCGAAAAATCAAG+AGG - chr2.4:67530838-67530857 MS.gene004377:CDS 30.0%
TACAAGATGATCTAACCTTA+AGG + chr2.4:67531621-67531640 None:intergenic 30.0%
TATGGTGCAGATAATCTTAT+TGG - chr2.4:67530632-67530651 MS.gene004377:CDS 30.0%
TCTAAACCTGTCTAAATTTC+AGG - chr2.4:67531525-67531544 MS.gene004377:CDS 30.0%
TCTAAATTCTCTTTGCATGA+TGG + chr2.4:67531235-67531254 None:intergenic 30.0%
TCTCCATGTATAACTGAAAT+TGG - chr2.4:67531409-67531428 MS.gene004377:CDS 30.0%
TGAAAACTCTCAAAATCCAA+GGG - chr2.4:67532378-67532397 MS.gene004377:CDS 30.0%
TGAATCGAAAGGAATGAAAT+CGG - chr2.4:67530982-67531001 MS.gene004377:CDS 30.0%
TTGAAAACTCTCAAAATCCA+AGG - chr2.4:67532377-67532396 MS.gene004377:CDS 30.0%
! AGATTGTATTCAGAGGATTT+TGG - chr2.4:67531345-67531364 MS.gene004377:CDS 30.0%
! CTCATAACAAAGTTTTGCTA+TGG - chr2.4:67530533-67530552 MS.gene004377:CDS 30.0%
! CTTGATTTTTCGCATCATTA+CGG + chr2.4:67530838-67530857 None:intergenic 30.0%
! TGTTATGAGCTCAAATGTTT+TGG + chr2.4:67530522-67530541 None:intergenic 30.0%
! TTCAAGGTTGTAAGATGTTT+TGG - chr2.4:67531688-67531707 MS.gene004377:CDS 30.0%
! TTTCCATTACTTTCTAGAAG+TGG - chr2.4:67530413-67530432 MS.gene004377:intron 30.0%
AAAAACTTCAGGCACATACA+TGG - chr2.4:67532170-67532189 MS.gene004377:intron 35.0%
AACTCCTTTCTTATGCTTCA+AGG - chr2.4:67531672-67531691 MS.gene004377:CDS 35.0%
AAGAGAACGTCTATTAGAGT+TGG - chr2.4:67532459-67532478 MS.gene004377:intron 35.0%
AATTCAAGGGTTTGTACAAC+AGG - chr2.4:67529711-67529730 MS.gene004377:CDS 35.0%
AATTGCACCCACTAATTCAT+TGG + chr2.4:67530196-67530215 None:intergenic 35.0%
ACAACCTTGAAGCATAAGAA+AGG + chr2.4:67531679-67531698 None:intergenic 35.0%
ACAATACCAATGAACCGTAT+CGG - chr2.4:67530068-67530087 MS.gene004377:intron 35.0%
ACGAAATTATAGTCACAGAG+AGG + chr2.4:67529979-67529998 None:intergenic 35.0%
ACTTGCCAATTGAACAAATC+TGG + chr2.4:67530809-67530828 None:intergenic 35.0%
ATTGCACCCACTAATTCATT+GGG + chr2.4:67530195-67530214 None:intergenic 35.0%
ATTTGTGATGATTTCGATGC+TGG - chr2.4:67531762-67531781 MS.gene004377:intron 35.0%
CAAAATCCAAGGGCAAATAT+CGG - chr2.4:67532388-67532407 MS.gene004377:CDS 35.0%
CGCAATAGATGTATACCTTA+AGG - chr2.4:67531603-67531622 MS.gene004377:CDS 35.0%
CTCCAAATGACTGAAAACTA+TGG - chr2.4:67530614-67530633 MS.gene004377:CDS 35.0%
GAATTGCCAAAAGCTATCAT+TGG - chr2.4:67532231-67532250 MS.gene004377:intron 35.0%
GAGAATTTAGAGAAAAGGGT+AGG - chr2.4:67531244-67531263 MS.gene004377:CDS 35.0%
GATCGTTTGTTTACGTAGTT+CGG - chr2.4:67529940-67529959 MS.gene004377:intron 35.0%
GATCTTCTCATTCCGAATAT+GGG - chr2.4:67531295-67531314 MS.gene004377:CDS 35.0%
GATGCACATAATGTTGAACA+TGG + chr2.4:67530154-67530173 None:intergenic 35.0%
GCTGGTAGATAGACATAAAA+TGG + chr2.4:67531372-67531391 None:intergenic 35.0%
GGGTTTACTAGAAGAAATTG+TGG - chr2.4:67531126-67531145 MS.gene004377:CDS 35.0%
TAAGGTATACATCTATTGCG+TGG + chr2.4:67531603-67531622 None:intergenic 35.0%
TATACATGGAGAAGCAGATA+AGG + chr2.4:67531401-67531420 None:intergenic 35.0%
TTCAGATACAAAGAACCAAC+CGG + chr2.4:67532353-67532372 None:intergenic 35.0%
TTTACGAAGAACTAAGATCG+TGG - chr2.4:67531726-67531745 MS.gene004377:intron 35.0%
! AATTTCTTTTCTCCTCCACA+AGG - chr2.4:67529767-67529786 MS.gene004377:CDS 35.0%
! AGTCCACTTCTAGAAAGTAA+TGG + chr2.4:67530419-67530438 None:intergenic 35.0%
! CACCATAGTTTTCAGTCATT+TGG + chr2.4:67530619-67530638 None:intergenic 35.0%
! GAGTTGATTCCTAATAAGAG+AGG - chr2.4:67531148-67531167 MS.gene004377:CDS 35.0%
! TTCCTTCCAATGATAGCTTT+TGG + chr2.4:67532240-67532259 None:intergenic 35.0%
!! AACTTCTGAAGCTGATCAAA+GGG - chr2.4:67531105-67531124 MS.gene004377:CDS 35.0%
!! AATACAAAAGTACCTTGTGG+AGG + chr2.4:67529782-67529801 None:intergenic 35.0%
!! TTCTAGATGCATTGATTCCT+TGG - chr2.4:67530763-67530782 MS.gene004377:CDS 35.0%
AACCACCAATTATCACCAAC+AGG + chr2.4:67530908-67530927 None:intergenic 40.0%
AAGTTAGCGACCATTGTCAT+CGG + chr2.4:67531466-67531485 None:intergenic 40.0%
ACATACATGGCTGACAGATT+CGG - chr2.4:67532183-67532202 MS.gene004377:intron 40.0%
ACTTGATCCATTCTCACTTG+TGG + chr2.4:67530465-67530484 None:intergenic 40.0%
ACTTGCACGTAATATCATCG+CGG + chr2.4:67531212-67531231 None:intergenic 40.0%
CAAGTGCCTGAAATTTAGAC+AGG + chr2.4:67531534-67531553 None:intergenic 40.0%
CATTCCGAATATGGGATACT+CGG - chr2.4:67531303-67531322 MS.gene004377:CDS 40.0%
GAGGAACATGAGTTGTTGAA+TGG - chr2.4:67530857-67530876 MS.gene004377:CDS 40.0%
GATCAGCTTCAGAAGTTGTA+GGG + chr2.4:67531102-67531121 None:intergenic 40.0%
GATGTGACACCTCTCTTATT+AGG + chr2.4:67531160-67531179 None:intergenic 40.0%
GCGATACCCAATGAATTAGT+GGG - chr2.4:67530186-67530205 MS.gene004377:intron 40.0%
GGATCTTCTCATTCCGAATA+TGG - chr2.4:67531294-67531313 MS.gene004377:CDS 40.0%
GTCATGCAGCACAAAATGAA+AGG - chr2.4:67532263-67532282 MS.gene004377:intron 40.0%
GTCTAAGCTGCTCGAAAAAT+AGG + chr2.4:67532315-67532334 None:intergenic 40.0%
TGATCAGCTTCAGAAGTTGT+AGG + chr2.4:67531103-67531122 None:intergenic 40.0%
TGCCAAAAGCTATCATTGGA+AGG - chr2.4:67532235-67532254 MS.gene004377:intron 40.0%
TGCGATACCCAATGAATTAG+TGG - chr2.4:67530185-67530204 MS.gene004377:intron 40.0%
TTGCAACTTCATGATGTTCC+TGG - chr2.4:67530491-67530510 MS.gene004377:intron 40.0%
TTGCAATTCCTGATTATGCC+AGG - chr2.4:67531557-67531576 MS.gene004377:CDS 40.0%
TTGTTCAATTGGCAAGTGAC+TGG - chr2.4:67530812-67530831 MS.gene004377:CDS 40.0%
! CGGATCCAGATTTGTTCAAT+TGG - chr2.4:67530801-67530820 MS.gene004377:CDS 40.0%
!! AAATGAGTGCTGACCATGTT+TGG + chr2.4:67529517-67529536 None:intergenic 40.0%
!! ACTTCTGAAGCTGATCAAAG+GGG - chr2.4:67531106-67531125 MS.gene004377:CDS 40.0%
!! CAACTTCTGAAGCTGATCAA+AGG - chr2.4:67531104-67531123 MS.gene004377:CDS 40.0%
!! CATTGATTCCTTGGCTATGA+AGG - chr2.4:67530772-67530791 MS.gene004377:CDS 40.0%
ACGAAGAACTAAGATCGTGG+AGG - chr2.4:67531729-67531748 MS.gene004377:intron 45.0%
ATACCAATGAACCGTATCGG+AGG - chr2.4:67530071-67530090 MS.gene004377:intron 45.0%
ATAGACACATAGTAGGGGCA+AGG + chr2.4:67530124-67530143 None:intergenic 45.0%
ATGGTCGCTAACTTGGTAGA+TGG - chr2.4:67531472-67531491 MS.gene004377:CDS 45.0%
CAACTTCATGATGTTCCTGG+TGG - chr2.4:67530494-67530513 MS.gene004377:intron 45.0%
CAATTATCACCAACAGGCTG+TGG + chr2.4:67530902-67530921 None:intergenic 45.0%
CACGTAATATCATCGCGGTT+CGG + chr2.4:67531207-67531226 None:intergenic 45.0%
CATCCTCTAAGCATCTCCTT+CGG - chr2.4:67531176-67531195 MS.gene004377:CDS 45.0%
GAAAGTAAACCACAGCCTGT+TGG - chr2.4:67530890-67530909 MS.gene004377:CDS 45.0%
GATGACAATGGTCGCTAACT+TGG - chr2.4:67531465-67531484 MS.gene004377:CDS 45.0%
GTGGTATATACCGTCGTCAA+GGG + chr2.4:67531584-67531603 None:intergenic 45.0%
TACCAATGAACCGTATCGGA+GGG - chr2.4:67530072-67530091 MS.gene004377:intron 45.0%
TACGTTGACACCGATGACAA+TGG - chr2.4:67531453-67531472 MS.gene004377:CDS 45.0%
TAGACACATAGTAGGGGCAA+GGG + chr2.4:67530123-67530142 None:intergenic 45.0%
TCAAGCGCTTTGATGGAATC+TGG + chr2.4:67530698-67530717 None:intergenic 45.0%
TCAAGGGAAGAAACCTCCTA+AGG + chr2.4:67531036-67531055 None:intergenic 45.0%
TCGACCGAGTATCCCATATT+CGG + chr2.4:67531310-67531329 None:intergenic 45.0%
TGGTATATACCGTCGTCAAG+GGG + chr2.4:67531583-67531602 None:intergenic 45.0%
! CTTGGCTATGAAGGCTTGTT+CGG - chr2.4:67530781-67530800 MS.gene004377:CDS 45.0%
!! AGCCTGTTGGTGATAATTGG+TGG - chr2.4:67530903-67530922 MS.gene004377:CDS 45.0%
!! CACAGCCTGTTGGTGATAAT+TGG - chr2.4:67530900-67530919 MS.gene004377:CDS 45.0%
!!! TCAAATGTTTTGGCTCCACC+AGG + chr2.4:67530512-67530531 None:intergenic 45.0%
AAGCAGATAAGGATGCAGGC+TGG + chr2.4:67531390-67531409 None:intergenic 50.0%
ACTAGACCAGGCTTCACTTG+TGG - chr2.4:67531273-67531292 MS.gene004377:CDS 50.0%
AGACACATAGTAGGGGCAAG+GGG + chr2.4:67530122-67530141 None:intergenic 50.0%
AGACTTCGCACGTCGATATC+CGG - chr2.4:67532331-67532350 MS.gene004377:intron 50.0%
ATCCACTTGACAACTCCCTG+AGG + chr2.4:67532416-67532435 None:intergenic 50.0%
ATGGCTGACAGATTCGGAAC+GGG - chr2.4:67532189-67532208 MS.gene004377:intron 50.0%
CAACCGAAGGAGATGCTTAG+AGG + chr2.4:67531182-67531201 None:intergenic 50.0%
CATGGCTGACAGATTCGGAA+CGG - chr2.4:67532188-67532207 MS.gene004377:intron 50.0%
CGAACAAGCCTTCATAGCCA+AGG + chr2.4:67530783-67530802 None:intergenic 50.0%
CGTGGTATATACCGTCGTCA+AGG + chr2.4:67531585-67531604 None:intergenic 50.0%
GAAGATCCACAAGTGAAGCC+TGG + chr2.4:67531282-67531301 None:intergenic 50.0%
GCACGTTTCAAGCGCTTTGA+TGG + chr2.4:67530705-67530724 None:intergenic 50.0%
GCAGAGTCCACAAGTGAGAA+TGG - chr2.4:67530455-67530474 MS.gene004377:intron 50.0%
GGAGAAGCAGATAAGGATGC+AGG + chr2.4:67531394-67531413 None:intergenic 50.0%
TAACGTAGCTCCCTCCGATA+CGG + chr2.4:67530085-67530104 None:intergenic 50.0%
TCCACTTGACAACTCCCTGA+GGG + chr2.4:67532415-67532434 None:intergenic 50.0%
! AGGCTTTGATCCCAGCTTCA+TGG + chr2.4:67529474-67529493 None:intergenic 50.0%
! CCTGAAGCATTTCGTCGTGA+GGG - chr2.4:67529491-67529510 MS.gene004377:CDS 50.0%
AGGGCAAATATCGGTCCCTC+AGG - chr2.4:67532397-67532416 MS.gene004377:intron 55.0%
CCCTCACGACGAAATGCTTC+AGG + chr2.4:67529494-67529513 None:intergenic 55.0%
CTCCCTCCGATACGGTTCAT+TGG + chr2.4:67530077-67530096 None:intergenic 55.0%
GAGGGACCGATATTTGCCCT+TGG + chr2.4:67532397-67532416 None:intergenic 55.0%
GGGCAAATATCGGTCCCTCA+GGG - chr2.4:67532398-67532417 MS.gene004377:intron 55.0%
GGTAGGAGCTCAACTAGACC+AGG - chr2.4:67531261-67531280 MS.gene004377:CDS 55.0%
TCAAGGGGCCTGGCATAATC+AGG + chr2.4:67531568-67531587 None:intergenic 55.0%
TCCCTCAGGGAGTTGTCAAG+TGG - chr2.4:67532411-67532430 MS.gene004377:intron 55.0%
TTCGCACGTCGATATCCGGT+TGG - chr2.4:67532335-67532354 MS.gene004377:intron 55.0%
TTCGTCGTGAGGGCCAAACA+TGG - chr2.4:67529501-67529520 MS.gene004377:CDS 55.0%
! CAAAGCGCTTGAAACGTGCG+AGG - chr2.4:67530706-67530725 MS.gene004377:CDS 55.0%
! GCCTGAAGCATTTCGTCGTG+AGG - chr2.4:67529490-67529509 MS.gene004377:CDS 55.0%
ACATAGTAGGGGCAAGGGGC+GGG + chr2.4:67530118-67530137 None:intergenic 60.0%
CACATAGTAGGGGCAAGGGG+CGG + chr2.4:67530119-67530138 None:intergenic 60.0%
TATACCGTCGTCAAGGGGCC+TGG + chr2.4:67531578-67531597 None:intergenic 60.0%
TATGCCAGGCCCCTTGACGA+CGG - chr2.4:67531571-67531590 MS.gene004377:CDS 60.0%
ATAGTAGGGGCAAGGGGCGG+GGG + chr2.4:67530116-67530135 None:intergenic 65.0%
CATAGTAGGGGCAAGGGGCG+GGG + chr2.4:67530117-67530136 None:intergenic 65.0%
! TCGCGGTTCGGAGCAACCGA+AGG + chr2.4:67531195-67531214 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 67529464 67532666 67529464 ID=MS.gene004377
chr2.4 mRNA 67529464 67532666 67529464 ID=MS.gene004377.t1;Parent=MS.gene004377
chr2.4 exon 67532608 67532666 67532608 ID=MS.gene004377.t1.exon1;Parent=MS.gene004377.t1
chr2.4 CDS 67532608 67532666 67532608 ID=cds.MS.gene004377.t1;Parent=MS.gene004377.t1
chr2.4 exon 67532342 67532411 67532342 ID=MS.gene004377.t1.exon2;Parent=MS.gene004377.t1
chr2.4 CDS 67532342 67532411 67532342 ID=cds.MS.gene004377.t1;Parent=MS.gene004377.t1
chr2.4 exon 67530506 67531717 67530506 ID=MS.gene004377.t1.exon3;Parent=MS.gene004377.t1
chr2.4 CDS 67530506 67531717 67530506 ID=cds.MS.gene004377.t1;Parent=MS.gene004377.t1
chr2.4 exon 67529464 67529949 67529464 ID=MS.gene004377.t1.exon4;Parent=MS.gene004377.t1
chr2.4 CDS 67529464 67529949 67529464 ID=cds.MS.gene004377.t1;Parent=MS.gene004377.t1
Gene Sequence

>MS.gene004377

ATGGCATCCATGAAGCTGGGATCAAAGCCTGAAGCATTTCGTCGTGAGGGCCAAACATGGTCAGCACTCATTTCTCTATATCAATATTCATCCTTATATCTTATATAATATGTGACATTAACAATGTGTGTAATTTTTTTGTTTCTGTCAATGTTCCTATGTCAAAATTGAACCTTAAATTTCATTTGATTTTTTTGGATAGTGAGAATAGGTTTCTCAATTGTATAATTGACCATGATCTTGTTTTAATTCAAGGGTTTGTACAACAGGACTTCCAAGTGATGTTACTATTGAAGTTGGCGAAATTTCTTTTCTCCTCCACAAGGTACTTTTGTATTTAACTTTAAAACTTATTGCACACTTTTTCTTAATGTAACATTAGTTAATTTATTTCAATTTTATCCTCAAGTTAATTATTAGAACAACTACTAGTAACAGATTATTAAAGAAAACTTAAATCATAAACAATGTAATGTGATCGTTTGTTTACGTAGTTCGGTCAATTGTGTCTGCCTCTCTGTGACTATAATTTCGTATATTACTTGAGTGAATTAGTGTTCAAAGTGTTACAAGCCGTATATATAGTACTAAAGTTCAGATTACAACAATACCAATGAACCGTATCGGAGGGAGCTACGTTAGCTCAGCTCCCCCGCCCCTTGCCCCTACTATGTGTCTATATATGAGCCATGTTCAACATTATGTGCATCGTCAAATTTTGTGCGATACCCAATGAATTAGTGGGTGCAATTTTTAAAATATCGAGACTCGTTCAAATTGCAACAAAAGTACAAAAATACAATGAAATGTGTGTTAAGAGAGTGTTTTTCTAAATTTCCAGCATTTATAATTAAATTTTTGTCTTTGTTGGTCTATGCAACATGTTAAATTAAAGTATCTAATTCATGTTGATCATTAGTTTTTTTCAACATTTGTGATTTTGATGCAGTTTCCATTACTTTCTAGAAGTGGACTACTAAAGAAACTAATTGCAGAGTCCACAAGTGAGAATGGATCAAGTAGTGTTTTGCAACTTCATGATGTTCCTGGTGGAGCCAAAACATTTGAGCTCATAACAAAGTTTTGCTATGGTGTGAAATTAGAAATCACAGCATTGAATGTAGTTAGTCTAAGATGTGCAGCAGAGTATCTCCAAATGACTGAAAACTATGGTGCAGATAATCTTATTGGTCACACAGAATCTTTTCTAAATGAAGTTTTCAGCAATTGGCCAGATTCCATCAAAGCGCTTGAAACGTGCGAGGAAGTGCAAAACTTTGCAGAAGATCTCCATATTGTTTCTAGATGCATTGATTCCTTGGCTATGAAGGCTTGTTCGGATCCAGATTTGTTCAATTGGCAAGTGACTGGCCGTAATGATGCGAAAAATCAAGAGGAACATGAGTTGTTGAATGGAATGTCTTGTGAAAGTAAACCACAGCCTGTTGGTGATAATTGGTGGTTTTATGATGTGTCTTTGTTGAGCCTGCCATTATATAAAAGATTGATTTTAGCTATTGAATCGAAAGGAATGAAATCGGAGACTATTGCTGCATCTCTTATATATTACCTTAGGAGGTTTCTTCCCTTGATGAATAGACAATCAAGTTTCGAAGATACAAATCATGCAACAATCCCTACAACTTCTGAAGCTGATCAAAGGGGTTTACTAGAAGAAATTGTGGAGTTGATTCCTAATAAGAGAGGTGTCACATCCTCTAAGCATCTCCTTCGGTTGCTCCGAACCGCGATGATATTACGTGCAAGTCCATCATGCAAAGAGAATTTAGAGAAAAGGGTAGGAGCTCAACTAGACCAGGCTTCACTTGTGGATCTTCTCATTCCGAATATGGGATACTCGGTCGAGACGCTCTATGATATAGATTGTATTCAGAGGATTTTGGACCATTTTATGTCTATCTACCAGCCTGCATCCTTATCTGCTTCTCCATGTATAACTGAAATTGGAACATTAATTGCTGGGGTTGATACGTTGACACCGATGACAATGGTCGCTAACTTGGTAGATGGATATCTCGCAGAAGTCGCTTCAGATGCTAATCTAAACCTGTCTAAATTTCAGGCACTTGCTGTTGCAATTCCTGATTATGCCAGGCCCCTTGACGACGGTATATACCACGCAATAGATGTATACCTTAAGGTTAGATCATCTTGTATACCTTTCTTTGCCAGTAATTTTTTTATATGAACTCCTTTCTTATGCTTCAAGGTTGTAAGATGTTTTGGCATAATAATAAAAAGTTTACGAAGAACTAAGATCGTGGAGGAAATAATGAGATTTGTGATGATTTCGATGCTGGTGAATTAATGAATCAAAAAATTGTCCCTAAATAAAAATCACAGATTAAATTTCTCAGTACATTTTTTTCTTCTCAAAATATACTTCTAATTACTGCTACTAAATGTTGAGAGATGAGAAGTAAGAGTTGAAGATATCATATACAAGATGATATTTTAGAAACATCTATACATAGAAGGGACATGTTAAATATACTACTACTTTGTTTAATATATGTACAAATTGCAAAACATGGTTATAATAAGAAAAAGAGGTAGCAAAATAAACTAAGAAAGTATGTTTGGTGAATGAACCTTTCACAAATTTAGTACCATGAAATCACAATCATGTACTAATTAAAATTTACAATTGTTTTCTTTTGTTCCTAATCATTTCAAAATGTAACAAAAAACTTCAGGCACATACATGGCTGACAGATTCGGAACGGGAACAATTATGCAGACTAATGAATTGCCAAAAGCTATCATTGGAAGGAAGCAGTCATGCAGCACAAAATGAAAGGTTGCCTCTAAGAGTAATTGTTCAAGTCCTATTTTTCGAGCAGCTTAGACTTCGCACGTCGATATCCGGTTGGTTCTTTGTATCTGAAAATCTTGAAAACTCTCAAAATCCAAGGGCAAATATCGGTCCCTCAGGGAGTTGTCAAGTGGATTCTGCAGAGACAACTGAGAATGTAAGAGAACGTCTATTAGAGTTGGAGAAAGAATGTTCAAGCATTCGAAAAGAGTTGCAGAAGCTAACAAAATCAAAGAAAAGTTGGAGCATTTTTCCAAAAAGATTTGGATTTAGAAGAAAATCAGGGTCTTCCAATCCTAATGAATCAAATAATGTAAATACATCATCATCATCTGTGAATAAAAATCCAAACCATGAAAGTAGTTGA
Protein sequence

>MS.gene004377.t1

MASMKLGSKPEAFRREGQTWVCTTGLPSDVTIEVGEISFLLHKFPLLSRSGLLKKLIAESTSENGSSSVLQLHDVPGGAKTFELITKFCYGVKLEITALNVVSLRCAAEYLQMTENYGADNLIGHTESFLNEVFSNWPDSIKALETCEEVQNFAEDLHIVSRCIDSLAMKACSDPDLFNWQVTGRNDAKNQEEHELLNGMSCESKPQPVGDNWWFYDVSLLSLPLYKRLILAIESKGMKSETIAASLIYYLRRFLPLMNRQSSFEDTNHATIPTTSEADQRGLLEEIVELIPNKRGVTSSKHLLRLLRTAMILRASPSCKENLEKRVGAQLDQASLVDLLIPNMGYSVETLYDIDCIQRILDHFMSIYQPASLSASPCITEIGTLIAGVDTLTPMTMVANLVDGYLAEVASDANLNLSKFQALAVAIPDYARPLDDGIYHAIDVYLKAHTWLTDSEREQLCRLMNCQKLSLEGSSHAAQNERLPLRVIVQVLFFEQLRLRTSISGWFFVSENLENSQNPRANIGPSGSCQVDSAETTENVRERLLELEKECSSIRKELQKLTKSKKSWSIFPKRFGFRRKSGSSNPNESNNVNTSSSSVNKNPNHESS