AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068040.t1 MTR_4g093850 99.253 669 5 0 1 669 1 669 0.0 1384
MS.gene068040.t1 MTR_2g009340 80.495 687 114 9 1 669 1 685 0.0 1101
MS.gene068040.t1 MTR_4g093850 97.200 500 14 0 1 500 1 500 0.0 1013
MS.gene068040.t1 MTR_8g090220 41.878 671 288 12 18 657 27 626 5.23e-173 508
MS.gene068040.t1 MTR_8g090220 45.404 544 222 11 18 543 27 513 3.08e-161 474
MS.gene068040.t1 MTR_2g084595 37.645 603 336 9 27 621 6 576 1.39e-139 422
MS.gene068040.t1 MTR_2g018750 39.675 615 315 13 19 625 1 567 5.89e-136 412
MS.gene068040.t1 MTR_5g015790 38.567 586 306 11 23 599 5 545 2.49e-133 406
MS.gene068040.t1 MTR_8g078900 35.969 645 333 12 34 633 10 619 3.09e-132 404
MS.gene068040.t1 MTR_5g016060 38.014 584 291 9 33 606 29 551 5.37e-129 395
MS.gene068040.t1 MTR_5g032780 40.476 546 275 10 23 545 5 523 1.09e-128 394
MS.gene068040.t1 MTR_1g094850 38.019 626 328 14 19 635 1 575 1.63e-128 393
MS.gene068040.t1 MTR_4g022330 36.557 610 334 9 33 639 24 583 3.74e-126 387
MS.gene068040.t1 MTR_8g078900 36.276 623 317 12 56 633 2 589 7.02e-125 384
MS.gene068040.t1 MTR_1g082690 35.884 588 285 10 29 610 43 544 1.49e-121 373
MS.gene068040.t1 MTR_1g014070 39.764 508 258 8 39 540 22 487 2.04e-121 375
MS.gene068040.t1 MTR_8g069330 40.809 544 241 11 23 545 5 488 1.76e-120 372
MS.gene068040.t1 MTR_1g064360 37.782 532 275 7 23 548 5 486 2.95e-120 370
MS.gene068040.t1 MTR_1g106430 37.316 544 274 9 23 550 5 497 4.23e-120 372
MS.gene068040.t1 MTR_8g078900 37.522 573 298 11 86 633 42 579 9.47e-120 370
MS.gene068040.t1 MTR_2g048390 41.754 479 245 7 19 490 1 452 4.50e-118 361
MS.gene068040.t1 MTR_4g095430 36.177 586 312 9 33 615 2 528 1.52e-116 361
MS.gene068040.t1 MTR_1g088885 33.180 651 356 13 38 669 31 621 2.46e-112 351
MS.gene068040.t1 MTR_4g134210 37.970 532 276 11 23 552 5 484 4.48e-112 350
MS.gene068040.t1 MTR_7g073980 35.233 579 307 13 40 608 29 549 4.73e-111 348
MS.gene068040.t1 MTR_2g084595 38.004 471 256 7 27 491 6 446 1.23e-106 331
MS.gene068040.t1 MTR_2g027410 36.585 533 274 8 23 552 5 476 1.32e-106 335
MS.gene068040.t1 MTR_7g108290 33.441 622 343 13 33 636 26 594 5.38e-106 336
MS.gene068040.t1 MTR_3g062540 33.007 612 323 12 33 640 13 541 5.08e-99 315
MS.gene068040.t1 MTR_5g075640 31.884 621 332 13 33 643 14 553 1.08e-97 312
MS.gene068040.t1 MTR_8g078900 36.961 487 244 9 34 490 10 463 3.82e-97 306
MS.gene068040.t1 MTR_2g027410 37.849 465 225 8 91 552 1 404 1.22e-92 296
MS.gene068040.t1 MTR_5g016060 36.016 497 247 9 120 606 2 437 5.63e-92 295
MS.gene068040.t1 MTR_3g062540 32.530 581 305 12 64 640 2 499 1.86e-87 283
MS.gene068040.t1 MTR_5g075640 31.017 590 316 13 64 643 2 510 8.34e-86 279
MS.gene068040.t1 MTR_8g078900 39.277 415 209 8 86 490 42 423 6.46e-85 273
MS.gene068040.t1 MTR_4g011530 36.407 423 206 9 207 623 26 391 6.27e-79 258
MS.gene068040.t1 MTR_2g102730 35.684 468 233 10 216 669 10 423 1.63e-78 256
MS.gene068040.t1 MTR_1g035720 29.245 530 260 15 47 539 6 457 7.35e-52 188
MS.gene068040.t1 MTR_4g074190 27.768 587 324 16 46 606 5 517 8.34e-52 188
MS.gene068040.t1 MTR_4g074190 27.440 543 298 12 86 606 3 471 2.83e-49 180
MS.gene068040.t1 MTR_4g045767 27.018 607 330 18 47 623 7 530 9.77e-49 179
MS.gene068040.t1 MTR_4g074190 26.850 473 264 13 46 494 5 419 7.88e-38 146
MS.gene068040.t1 MTR_1g105870 35.931 231 117 4 51 279 38 239 7.60e-35 133
MS.gene068040.t1 MTR_2g010890 24.945 457 270 7 110 559 1 391 1.74e-31 128
MS.gene068040.t1 MTR_2g048375 37.879 132 72 2 507 635 1 125 5.65e-21 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068040.t1 AT5G64330 70.139 720 144 12 1 649 2 721 0.0 960
MS.gene068040.t1 AT5G64330 70.279 646 124 11 1 578 2 647 0.0 860
MS.gene068040.t1 AT5G64330 71.012 583 108 9 57 578 6 588 0.0 785
MS.gene068040.t1 AT5G10250 40.063 634 273 13 30 637 36 588 1.03e-151 453
MS.gene068040.t1 AT1G30440 38.779 655 356 11 19 654 1 629 9.73e-150 449
MS.gene068040.t1 AT5G10250 40.128 623 273 13 30 637 36 573 4.70e-148 443
MS.gene068040.t1 AT5G03250 41.682 535 267 8 25 547 7 508 2.51e-135 410
MS.gene068040.t1 AT3G44820 36.153 603 349 7 27 618 6 583 9.28e-130 397
MS.gene068040.t1 AT5G13600 39.286 616 302 17 19 623 1 555 1.12e-129 395
MS.gene068040.t1 AT2G14820 38.447 541 272 9 23 550 5 497 4.85e-125 385
MS.gene068040.t1 AT2G14820 38.447 541 272 9 23 550 5 497 4.85e-125 385
MS.gene068040.t1 AT5G67440 39.810 525 257 8 23 541 5 476 2.10e-123 379
MS.gene068040.t1 AT5G67440 39.810 525 257 8 23 541 5 476 2.10e-123 379
MS.gene068040.t1 AT5G67385 39.731 521 260 8 33 550 14 483 2.05e-122 377
MS.gene068040.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene068040.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene068040.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene068040.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene068040.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene068040.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene068040.t1 AT5G66560 33.728 676 337 13 34 640 9 642 1.00e-115 362
MS.gene068040.t1 AT4G37590 37.879 594 269 15 23 599 5 515 1.99e-115 358
MS.gene068040.t1 AT4G31820 37.729 546 269 13 23 562 5 485 8.84e-115 356
MS.gene068040.t1 AT5G67440 40.164 488 234 7 59 541 5 439 1.04e-114 355
MS.gene068040.t1 AT5G48800 35.489 603 330 7 37 636 34 580 1.29e-114 357
MS.gene068040.t1 AT1G03010 33.385 644 348 14 23 641 16 603 1.70e-112 352
MS.gene068040.t1 AT3G08570 35.197 608 326 11 40 640 30 576 4.02e-112 350
MS.gene068040.t1 AT1G03010 33.760 625 334 13 42 641 7 576 2.68e-111 348
MS.gene068040.t1 AT1G03010 33.760 625 334 13 42 641 33 602 7.54e-111 348
MS.gene068040.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene068040.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene068040.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene068040.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene068040.t1 AT2G30520 34.975 589 293 13 34 615 20 525 2.40e-108 340
MS.gene068040.t1 AT5G66560 34.098 654 320 13 56 640 2 613 3.21e-108 341
MS.gene068040.t1 AT2G47860 34.500 600 315 10 38 618 32 572 9.67e-108 340
MS.gene068040.t1 AT5G47800 36.902 523 271 9 23 539 5 474 1.57e-107 337
MS.gene068040.t1 AT5G47800 36.902 523 271 9 23 539 5 474 1.57e-107 337
MS.gene068040.t1 AT5G47800 36.902 523 271 9 23 539 5 474 1.57e-107 337
MS.gene068040.t1 AT3G26490 36.823 554 268 12 25 545 7 511 2.20e-107 337
MS.gene068040.t1 AT3G26490 36.823 554 268 12 25 545 7 511 2.20e-107 337
MS.gene068040.t1 AT3G26490 36.823 554 268 12 25 545 7 511 2.20e-107 337
MS.gene068040.t1 AT3G08660 35.043 585 287 13 40 615 30 530 1.39e-106 335
MS.gene068040.t1 AT5G47800 36.902 523 271 9 23 539 118 587 7.62e-106 336
MS.gene068040.t1 AT5G66560 36.034 580 304 11 86 640 66 603 9.49e-105 332
MS.gene068040.t1 AT2G47860 33.226 623 315 11 38 618 32 595 1.22e-102 327
MS.gene068040.t1 AT4G31820 37.223 497 242 12 71 562 1 432 3.32e-99 314
MS.gene068040.t1 AT2G23050 35.000 540 255 12 23 553 5 457 2.81e-97 308
MS.gene068040.t1 AT2G30520 34.168 559 278 13 64 615 2 477 1.42e-95 305
MS.gene068040.t1 AT2G30520 34.168 559 278 13 64 615 2 477 1.42e-95 305
MS.gene068040.t1 AT3G08660 34.477 554 270 13 71 615 1 470 1.60e-95 304
MS.gene068040.t1 AT3G50840 33.114 607 321 13 38 633 10 542 9.72e-95 304
MS.gene068040.t1 AT5G03250 39.627 429 216 7 129 547 1 396 3.84e-92 294
MS.gene068040.t1 AT3G49970 33.694 555 258 11 56 608 6 452 7.44e-88 284
MS.gene068040.t1 AT3G49970 33.694 555 258 11 56 608 6 452 7.44e-88 284
MS.gene068040.t1 AT3G50840 32.815 579 306 12 65 633 1 506 9.23e-83 271
MS.gene068040.t1 AT3G50840 32.815 579 306 12 65 633 1 506 9.23e-83 271
MS.gene068040.t1 AT3G26490 34.600 500 245 12 25 491 7 457 2.11e-82 269
MS.gene068040.t1 AT5G66560 33.852 514 242 11 34 491 9 480 2.51e-82 268
MS.gene068040.t1 AT2G47860 34.783 483 214 9 38 490 32 443 4.53e-80 261
MS.gene068040.t1 AT1G52770 36.183 503 232 16 205 669 3 454 6.04e-80 261
MS.gene068040.t1 AT3G08660 33.978 465 230 9 40 498 30 423 2.87e-79 259
MS.gene068040.t1 AT3G15570 34.047 467 238 9 230 669 29 452 2.74e-76 251
MS.gene068040.t1 AT2G47860 33.535 495 229 10 38 491 32 467 4.02e-75 249
MS.gene068040.t1 AT2G47860 31.041 509 277 8 129 618 1 454 7.12e-69 234
MS.gene068040.t1 AT2G47860 31.041 509 277 8 129 618 1 454 7.12e-69 234
MS.gene068040.t1 AT2G23050 33.879 428 193 10 129 553 1 341 6.56e-65 219
MS.gene068040.t1 AT3G19850 28.355 529 280 9 44 540 3 464 2.96e-56 201
MS.gene068040.t1 AT1G50280 28.487 509 292 13 46 535 5 460 8.81e-49 179
MS.gene068040.t1 AT1G50280 29.374 463 261 11 86 535 18 427 1.00e-47 176
MS.gene068040.t1 AT5G17580 28.161 522 298 12 40 553 1 453 9.66e-44 166
MS.gene068040.t1 AT5G17580 27.969 522 299 12 40 553 16 468 1.16e-43 166
MS.gene068040.t1 AT3G22104 26.477 491 275 13 46 526 6 420 4.37e-39 151
MS.gene068040.t1 AT3G03510 26.799 556 278 17 94 642 4 437 8.01e-38 147
MS.gene068040.t1 AT5G48130 32.911 237 145 4 37 269 32 258 3.89e-34 138
MS.gene068040.t1 AT5G48130 30.049 203 113 4 436 614 364 561 2.27e-19 92.8
MS.gene068040.t1 AT3G22104 24.821 419 232 10 115 526 3 345 1.08e-25 110
MS.gene068040.t1 AT3G22104 24.821 419 232 10 115 526 3 345 1.08e-25 110
MS.gene068040.t1 AT3G22104 24.821 419 232 10 115 526 3 345 1.08e-25 110
MS.gene068040.t1 AT3G49900 36.250 160 84 5 34 184 55 205 6.34e-14 75.5
MS.gene068040.t1 AT3G49900 33.981 103 63 3 445 546 214 312 4.34e-11 66.2
MS.gene068040.t1 AT3G49900 36.250 160 84 5 34 184 55 205 6.39e-14 75.5

Find 138 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCATTTCGTTCCATTT+TGG 0.129719 4.3:-20743078 None:intergenic
CTGTTCGGTTCTTCAGAAAT+TGG 0.136199 4.3:-20745178 None:intergenic
GGACTTCATATGATTGTTTC+TGG 0.160796 4.3:+20743369 MS.gene068040:CDS
CCACTCTTGCCTTGGCATTC+AGG 0.181350 4.3:-20743849 None:intergenic
CCTTTCATCGACAAAGTTTC+AGG 0.183368 4.3:+20743918 MS.gene068040:CDS
ACAGCAGCTGGAAGGGAATA+TGG 0.207369 4.3:+20741919 MS.gene068040:CDS
TAGAGTCATTACTGCTATTA+AGG 0.220033 4.3:+20743182 MS.gene068040:CDS
GGAAGCTGGCTTCTCCAATT+TGG 0.236985 4.3:-20742182 None:intergenic
AAAATGAACGACTGCCATTA+AGG 0.241454 4.3:+20744792 MS.gene068040:CDS
TACGCCCACCTTATTCCTTT+TGG 0.245910 4.3:-20743027 None:intergenic
TAGCTGTTGTCCATCCTTCT+TGG 0.246421 4.3:-20744958 None:intergenic
ACTTGCACTACTACCCTTAA+TGG 0.258617 4.3:-20744806 None:intergenic
TTTGGAGCTTCAACATGATA+TGG 0.264232 4.3:+20745033 MS.gene068040:CDS
GAGTGTGAAGACAAAATATT+TGG 0.272726 4.3:+20745015 MS.gene068040:CDS
ATTACTGCTATTAAGGTAAA+AGG 0.292860 4.3:+20743189 MS.gene068040:CDS
TAATGCATTATGCAACTAAA+TGG 0.300901 4.3:+20743241 MS.gene068040:CDS
AGCAACTCCATAGCAGAATT+TGG 0.307927 4.3:-20742758 None:intergenic
TTGACAGCAAGCAACATTTC+CGG 0.311281 4.3:+20742787 MS.gene068040:CDS
TGAAGCTTTCGTCGCTCTTC+CGG 0.314769 4.3:-20743052 None:intergenic
TTCAATCTTAACACCATGTT+TGG 0.322863 4.3:-20741962 None:intergenic
GAGAATGGCAAACATGTTAA+AGG 0.326315 4.3:+20743512 MS.gene068040:CDS
GCTGCAAGTTCAAATGCTTC+AGG 0.329025 4.3:-20742736 None:intergenic
CTAGCCAACAGCTCTCTAAA+AGG 0.341374 4.3:+20744863 MS.gene068040:CDS
TGTTGCTTGTTTCCTCTCCT+TGG 0.344019 4.3:-20743296 None:intergenic
CGAAAGCTTCAAGCCCAAAA+TGG 0.344305 4.3:+20743064 MS.gene068040:CDS
AGTGATCTTCAAGTCCAAAT+TGG 0.350920 4.3:+20742168 MS.gene068040:CDS
CCTTCTCAGGCTTCTGAGAA+TGG 0.376751 4.3:+20743497 MS.gene068040:CDS
GTGCTAATCCAAAAGGAATA+AGG 0.382939 4.3:+20743019 MS.gene068040:CDS
AACAGGAAAACACTTCTAGA+AGG 0.384225 4.3:+20744920 MS.gene068040:CDS
CAGCAGCTGGAAGGGAATAT+GGG 0.385850 4.3:+20741920 MS.gene068040:CDS
GATACTCAGCAGCACATCTT+AGG 0.386146 4.3:-20742810 None:intergenic
TCTCGTGTAGCTTCCTTCTC+AGG 0.398409 4.3:+20743484 MS.gene068040:CDS
TTGAAGATGTTTCAATCCTT+AGG 0.401499 4.3:+20743145 MS.gene068040:CDS
TCAGAAAAGGATTGTCTACT+TGG 0.415289 4.3:-20743805 None:intergenic
CTTCTAGTTGTCTTTCTGTT+CGG 0.415753 4.3:-20745193 None:intergenic
AAATGTGGAAAATCATGATA+TGG 0.424301 4.3:+20745153 MS.gene068040:CDS
CTTTCATGCTCAGTTAGTGT+TGG 0.426046 4.3:-20744704 None:intergenic
TCCTGTTTGTTACCATTGGT+TGG 0.426066 4.3:-20744904 None:intergenic
GTGTCGTTCCCCGCCAGTCC+TGG 0.426869 4.3:-20743267 None:intergenic
CTATAGACTCGCTGCATCTT+CGG 0.430254 4.3:-20742984 None:intergenic
AACTAAATGGCTACCAGGAC+TGG 0.435309 4.3:+20743254 MS.gene068040:CDS
ATAGCTATCTTATTCAAGTC+GGG 0.438601 4.3:-20742694 None:intergenic
AAGCCTTGCCACTCTTGCCT+TGG 0.438871 4.3:-20743857 None:intergenic
AAACATGGTGTTAAGATTGA+AGG 0.441638 4.3:+20741964 MS.gene068040:CDS
TCAAATGCTTCAGGCCCTCC+AGG 0.443506 4.3:-20742727 None:intergenic
AAATGAACGACTGCCATTAA+GGG 0.444360 4.3:+20744793 MS.gene068040:CDS
AGCTAGTGCCTCCTGATTGG+TGG 0.449333 4.3:+20743121 MS.gene068040:CDS
CCTGAAACTTTGTCGATGAA+AGG 0.449456 4.3:-20743918 None:intergenic
TGCGTGCATGCAGGCGTCAA+TGG 0.453590 4.3:-20744765 None:intergenic
GGCACTAGCTGATTCCTGCT+CGG 0.457366 4.3:-20743108 None:intergenic
ATCAGCTAGTGCCTCCTGAT+TGG 0.466020 4.3:+20743118 MS.gene068040:CDS
CACATGCTTGTGATCAGAAA+AGG 0.471840 4.3:-20743818 None:intergenic
ACCAACCAATGGTAACAAAC+AGG 0.475079 4.3:+20744903 MS.gene068040:CDS
TGATTACTGATTTAGAGAAA+AGG 0.477093 4.3:+20743641 MS.gene068040:CDS
TGCAGAAGCTTTACCTGAGT+CGG 0.477481 4.3:+20743945 MS.gene068040:CDS
GATTACTGATTTAGAGAAAA+GGG 0.477757 4.3:+20743642 MS.gene068040:CDS
ACATCTTCAAACCACCAATC+AGG 0.482701 4.3:-20743132 None:intergenic
CTTAACACCATGTTTGGTTG+AGG 0.483711 4.3:-20741956 None:intergenic
AGATTTGAACTGATTGGTGC+TGG 0.486553 4.3:+20743216 MS.gene068040:CDS
TACCTTGTGCAAGTGGAAGC+TGG 0.487213 4.3:-20742196 None:intergenic
AAATGGCTACCAGGACTGGC+GGG 0.494907 4.3:+20743258 MS.gene068040:CDS
AAAGCTTGTGCTAATCCAAA+AGG 0.497825 4.3:+20743012 MS.gene068040:CDS
ATAGCTATGGATGATCTCCC+TGG 0.498197 4.3:+20742709 MS.gene068040:CDS
ATTTGGACTTGAAGATCACT+TGG 0.499439 4.3:-20742165 None:intergenic
CGGGGTCGCGAGAATCATAT+AGG 0.499847 4.3:-20742675 None:intergenic
GCACTAGCTGATTCCTGCTC+GGG 0.500897 4.3:-20743107 None:intergenic
GAAGCGTCTCTGTCGTGTAA+TGG 0.510244 4.3:+20744727 MS.gene068040:CDS
GAAAAGATACCTTGTGCAAG+TGG 0.511668 4.3:-20742203 None:intergenic
CAACACTAACTGAGCATGAA+AGG 0.512884 4.3:+20744705 MS.gene068040:CDS
GGATCTTGTTCAGAGGTTGT+TGG 0.513765 4.3:+20743744 MS.gene068040:CDS
GGAATAAGGTGGGCGTATAC+CGG 0.514993 4.3:+20743033 MS.gene068040:CDS
GGCAAGAGTGGCAAGGCTTG+TGG 0.518056 4.3:+20743861 MS.gene068040:CDS
GCGGTGACAACAACAGTAGC+TGG 0.519169 4.3:+20743340 MS.gene068040:CDS
CATAGCTATCTTATTCAAGT+CGG 0.522649 4.3:-20742695 None:intergenic
ACTGATTTAGAGAAAAGGGT+TGG 0.526187 4.3:+20743646 MS.gene068040:CDS
AAGTGAAACAGCAGCTGGAA+GGG 0.532024 4.3:+20741912 MS.gene068040:CDS
GGTATGAGATTTGAACTGAT+TGG 0.534123 4.3:+20743210 MS.gene068040:CDS
CGACTTGAATAAGATAGCTA+TGG 0.536962 4.3:+20742696 MS.gene068040:CDS
AGCAAAAGCACACATCAGCT+TGG 0.538253 4.3:+20745089 MS.gene068040:CDS
CCTGAATGCCAAGGCAAGAG+TGG 0.539436 4.3:+20743849 MS.gene068040:CDS
TAACAAAGTGATCAATCCTA+AGG 0.539725 4.3:-20743161 None:intergenic
ATGACTGAGGATCTAGAAGA+AGG 0.540350 4.3:+20742838 MS.gene068040:CDS
CGCAATCTTTCCAAGAAGGA+TGG 0.541041 4.3:+20744948 MS.gene068040:CDS
CGAACAGAAAGACAACTAGA+AGG 0.543947 4.3:+20745194 MS.gene068040:CDS
CACACATCAGCTTGGACTAG+TGG 0.544231 4.3:+20745097 MS.gene068040:CDS
TGAAAGACTCAAGCCCGAGC+AGG 0.544479 4.3:+20743094 MS.gene068040:CDS
CTCTGAAAAGCTGTCACCGT+GGG 0.544588 4.3:+20742942 MS.gene068040:CDS
CTTGCAGCCAAATTCTGCTA+TGG 0.546691 4.3:+20742751 MS.gene068040:CDS
CTTGTTCAAGAACAAACTGA+AGG 0.548683 4.3:+20743775 MS.gene068040:CDS
TCAAACCAAGGATCAAAAGA+TGG 0.555075 4.3:+20743416 MS.gene068040:CDS
TCAGAAAGTGAAACAGCAGC+TGG 0.555263 4.3:+20741907 MS.gene068040:CDS
TATCCTTTGCTATCTAGAAG+TGG 0.555707 4.3:+20742637 MS.gene068040:CDS
CTCAGCAGCACATCTTAGGC+CGG 0.556722 4.3:-20742806 None:intergenic
CCATTCTCAGAAGCCTGAGA+AGG 0.562143 4.3:-20743497 None:intergenic
TTCATCAAAGAGGCAACTCA+TGG 0.562485 4.3:+20741989 MS.gene068040:CDS
TCAGAAACTCTAGCCGACTC+AGG 0.569094 4.3:-20743958 None:intergenic
AGCAATTGATTCATATCTCA+AGG 0.570287 4.3:+20743996 MS.gene068040:CDS
AAAGTGAAACAGCAGCTGGA+AGG 0.573228 4.3:+20741911 MS.gene068040:CDS
AGCAGCTGGAAGGGAATATG+GGG 0.577271 4.3:+20741921 MS.gene068040:CDS
ACTTACTAAAATGACAAATG+TGG 0.586018 4.3:+20745138 MS.gene068040:CDS
CATCCTTCTTGGAAAGATTG+CGG 0.587019 4.3:-20744947 None:intergenic
ACACATCAGCTTGGACTAGT+GGG 0.588970 4.3:+20745098 MS.gene068040:CDS
GATGCAGCGAGTCTATAGCT+TGG 0.589137 4.3:+20742989 MS.gene068040:CDS
ACAGGAAAACACTTCTAGAA+GGG 0.589157 4.3:+20744921 MS.gene068040:CDS
AACAACAGTAGCTGGAGAGG+TGG 0.595238 4.3:+20743348 MS.gene068040:CDS
TAAATGGCTACCAGGACTGG+CGG 0.596021 4.3:+20743257 MS.gene068040:CDS
GTTCTCACCTCAACCAAACA+TGG 0.597650 4.3:+20741949 MS.gene068040:CDS
CTATGGATGATCTCCCTGGA+GGG 0.599558 4.3:+20742713 MS.gene068040:CDS
TTTCCACTTCTAGATAGCAA+AGG 0.603021 4.3:-20742640 None:intergenic
AATGGCTACCAGGACTGGCG+GGG 0.608558 4.3:+20743259 MS.gene068040:CDS
CAAATGCTTCAGGCCCTCCA+GGG 0.613888 4.3:-20742726 None:intergenic
ACACCGCAATCTTTCCAAGA+AGG 0.615108 4.3:+20744944 MS.gene068040:CDS
GGGAACGACACTACAATCCA+AGG 0.616043 4.3:+20743279 MS.gene068040:CDS
AGCCAGCTTCCACTTGCACA+AGG 0.623189 4.3:+20742194 MS.gene068040:CDS
ATGCCAAGGCAAGAGTGGCA+AGG 0.625949 4.3:+20743854 MS.gene068040:CDS
TCTGATCACAAGCATGTGGG+AGG 0.626741 4.3:+20743823 MS.gene068040:CDS
CTAATCCAAAAGGAATAAGG+TGG 0.630264 4.3:+20743022 MS.gene068040:CDS
GACAACAACAGTAGCTGGAG+AGG 0.638998 4.3:+20743345 MS.gene068040:CDS
TAGCTATCTTATTCAAGTCG+GGG 0.642768 4.3:-20742693 None:intergenic
ACAGAAAGACAACTAGAAGG+TGG 0.644997 4.3:+20745197 MS.gene068040:CDS
TGAATCTCAGTACCAACCAA+TGG 0.650255 4.3:+20744892 MS.gene068040:CDS
AACTTCAAGTCTTCAAACCA+AGG 0.650442 4.3:+20743404 MS.gene068040:CDS
TATGCAACTAAATGGCTACC+AGG 0.651310 4.3:+20743249 MS.gene068040:CDS
AAGTGAAACTATGTATGATG+TGG 0.652037 4.3:+20743723 MS.gene068040:CDS
CGACACTACAATCCAAGGAG+AGG 0.656624 4.3:+20743284 MS.gene068040:CDS
TCGGCTAGAGTTTCTGACGA+TGG 0.658303 4.3:+20743964 MS.gene068040:CDS
GGGAGGTAACCTGAATGCCA+AGG 0.660203 4.3:+20743840 MS.gene068040:CDS
TGTGGACAGTTATCTTACAG+AGG 0.662209 4.3:+20743879 MS.gene068040:CDS
GAGACACTGTCTTTCTGCGG+CGG 0.666070 4.3:-20743465 None:intergenic
CATCAGCTTGGACTAGTGGG+TGG 0.666857 4.3:+20745101 MS.gene068040:CDS
TAATCCAAAAGGAATAAGGT+GGG 0.670549 4.3:+20743023 MS.gene068040:CDS
TCTTCAAACCACCAATCAGG+AGG 0.670951 4.3:-20743129 None:intergenic
CACGAGACACTGTCTTTCTG+CGG 0.688596 4.3:-20743468 None:intergenic
GCTCTGAAAAGCTGTCACCG+TGG 0.705207 4.3:+20742941 MS.gene068040:CDS
ATGATGTGGATCTTGTTCAG+AGG 0.708047 4.3:+20743737 MS.gene068040:CDS
GCTATGGATGATCTCCCTGG+AGG 0.717645 4.3:+20742712 MS.gene068040:CDS
TGAGTATCTTGAAATGACTG+AGG 0.729252 4.3:+20742825 MS.gene068040:CDS
GCAGCTGGAAGGGAATATGG+GGG 0.752997 4.3:+20741922 MS.gene068040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATAACTTTCT+AGG - chr4.3:20742260-20742279 None:intergenic 10.0%
!! ATATGACAATATACTTATTT+AGG + chr4.3:20744231-20744250 MS.gene068040:intron 15.0%
!! TTCAATTAACTTACATAAAA+TGG - chr4.3:20742103-20742122 None:intergenic 15.0%
!!! GTTATTTTATTTTTTCTCTA+AGG + chr4.3:20742265-20742284 MS.gene068040:intron 15.0%
!!! TTTTTATTTTCAATTACTCT+AGG + chr4.3:20744074-20744093 MS.gene068040:intron 15.0%
!! AATTAGTTAGACATTGTTTA+AGG - chr4.3:20743580-20743599 None:intergenic 20.0%
!! CTAATATTTCAAGATAACAT+TGG + chr4.3:20744295-20744314 MS.gene068040:intron 20.0%
!!! TAGTTTTTTTCTTAACCTTT+CGG - chr4.3:20742544-20742563 None:intergenic 20.0%
! AAAGATATAATTGCGATGAA+GGG - chr4.3:20744338-20744357 None:intergenic 25.0%
! AAATGTGGAAAATCATGATA+TGG + chr4.3:20745153-20745172 MS.gene068040:CDS 25.0%
! AATATCAAACTATCTCTAAC+AGG + chr4.3:20743606-20743625 MS.gene068040:intron 25.0%
! ACATAAGACATCAATGAAAT+TGG - chr4.3:20744569-20744588 None:intergenic 25.0%
! ACTTACTAAAATGACAAATG+TGG + chr4.3:20745138-20745157 MS.gene068040:CDS 25.0%
! ATATTTCAAGATAACATTGG+AGG + chr4.3:20744298-20744317 MS.gene068040:intron 25.0%
! ATTACTGCTATTAAGGTAAA+AGG + chr4.3:20743189-20743208 MS.gene068040:CDS 25.0%
! CAATTAAGGATGATTCATTT+GGG + chr4.3:20742421-20742440 MS.gene068040:intron 25.0%
! CTCATTGAAAATGAAATTTG+AGG - chr4.3:20744379-20744398 None:intergenic 25.0%
! CTTATAATCTATAAACCGAA+AGG + chr4.3:20742526-20742545 MS.gene068040:intron 25.0%
! CTTTCGGTTTATAGATTATA+AGG - chr4.3:20742528-20742547 None:intergenic 25.0%
! TAAAGATATAATTGCGATGA+AGG - chr4.3:20744339-20744358 None:intergenic 25.0%
! TAATGCATTATGCAACTAAA+TGG + chr4.3:20743241-20743260 MS.gene068040:CDS 25.0%
! TCAATTAAGGATGATTCATT+TGG + chr4.3:20742420-20742439 MS.gene068040:intron 25.0%
! TCTTAAAAGCAAAGTTGTAT+TGG + chr4.3:20744162-20744181 MS.gene068040:intron 25.0%
!!! AATCACTTTTCAAAGGATAA+AGG + chr4.3:20744024-20744043 MS.gene068040:intron 25.0%
!!! AGTAATAGTAGTTTTAGTAG+CGG + chr4.3:20743321-20743340 MS.gene068040:CDS 25.0%
!!! ATCACTTTTCAAAGGATAAA+GGG + chr4.3:20744025-20744044 MS.gene068040:intron 25.0%
!!! GATTACTGATTTAGAGAAAA+GGG + chr4.3:20743642-20743661 MS.gene068040:CDS 25.0%
!!! GGTAATTAATCACTTTTCAA+AGG + chr4.3:20744017-20744036 MS.gene068040:intron 25.0%
!!! TGATTACTGATTTAGAGAAA+AGG + chr4.3:20743641-20743660 MS.gene068040:CDS 25.0%
AAACTCATTAACAACTTGAG+CGG - chr4.3:20742329-20742348 None:intergenic 30.0%
AAGTGAAACTATGTATGATG+TGG + chr4.3:20743723-20743742 MS.gene068040:CDS 30.0%
ACCAAACATACACTCAATTA+AGG + chr4.3:20742407-20742426 MS.gene068040:intron 30.0%
AGATTCACATGATATGATCA+TGG + chr4.3:20744630-20744649 MS.gene068040:intron 30.0%
AGGATACTTTCACAACAAAA+AGG - chr4.3:20742623-20742642 None:intergenic 30.0%
AGGTAGTTACAAGTTAGTTA+TGG - chr4.3:20743560-20743579 None:intergenic 30.0%
ATAGCTATCTTATTCAAGTC+GGG - chr4.3:20742697-20742716 None:intergenic 30.0%
CATAGCTATCTTATTCAAGT+CGG - chr4.3:20742698-20742717 None:intergenic 30.0%
GAGTGTGAAGACAAAATATT+TGG + chr4.3:20745015-20745034 MS.gene068040:CDS 30.0%
GATCTGTGAAACAAATTAAC+AGG + chr4.3:20744676-20744695 MS.gene068040:intron 30.0%
GTAAAATTTGAGTCAGAACT+TGG + chr4.3:20744112-20744131 MS.gene068040:intron 30.0%
GTTTATAGATTATAAGGACC+TGG - chr4.3:20742522-20742541 None:intergenic 30.0%
TAACAAAGTGATCAATCCTA+AGG - chr4.3:20743164-20743183 None:intergenic 30.0%
TAATCCAAAAGGAATAAGGT+GGG + chr4.3:20743023-20743042 MS.gene068040:CDS 30.0%
TAATGAGTTTCAGTGATGTT+TGG + chr4.3:20742339-20742358 MS.gene068040:intron 30.0%
TAGAGTCATTACTGCTATTA+AGG + chr4.3:20743182-20743201 MS.gene068040:CDS 30.0%
TCCTTAATTGAGTGTATGTT+TGG - chr4.3:20742411-20742430 None:intergenic 30.0%
TTCAATCTTAACACCATGTT+TGG - chr4.3:20741965-20741984 None:intergenic 30.0%
TTGAAGATGTTTCAATCCTT+AGG + chr4.3:20743145-20743164 MS.gene068040:CDS 30.0%
! AAACATGGTGTTAAGATTGA+AGG + chr4.3:20741964-20741983 MS.gene068040:CDS 30.0%
! AATAACCATCTTTTGATCCT+TGG - chr4.3:20743424-20743443 None:intergenic 30.0%
! AGCAATTGATTCATATCTCA+AGG + chr4.3:20743996-20744015 MS.gene068040:CDS 30.0%
! ATTGAAGGTTTTCATCAAAG+AGG + chr4.3:20741979-20741998 MS.gene068040:CDS 30.0%
! CTTTCATTTCGTTCCATTTT+GGG - chr4.3:20743080-20743099 None:intergenic 30.0%
! TCTTTCATTTCGTTCCATTT+TGG - chr4.3:20743081-20743100 None:intergenic 30.0%
!! CTCATTTTAGAAGAATGAGT+CGG + chr4.3:20744590-20744609 MS.gene068040:intron 30.0%
!! TCACTTTTCAAAGGATAAAG+GGG + chr4.3:20744026-20744045 MS.gene068040:intron 30.0%
!!! AAGCAAAGTTGTATTGGTTT+TGG + chr4.3:20744168-20744187 MS.gene068040:intron 30.0%
AAAATGAACGACTGCCATTA+AGG + chr4.3:20744792-20744811 MS.gene068040:CDS 35.0%
AAAGCTTGTGCTAATCCAAA+AGG + chr4.3:20743012-20743031 MS.gene068040:CDS 35.0%
AAATGAACGACTGCCATTAA+GGG + chr4.3:20744793-20744812 MS.gene068040:CDS 35.0%
AACAGGAAAACACTTCTAGA+AGG + chr4.3:20744920-20744939 MS.gene068040:CDS 35.0%
AACTTCAAGTCTTCAAACCA+AGG + chr4.3:20743404-20743423 MS.gene068040:CDS 35.0%
AGTGATCTTCAAGTCCAAAT+TGG + chr4.3:20742168-20742187 MS.gene068040:CDS 35.0%
AGTGATGTTTGGTATCAAGT+TGG + chr4.3:20742350-20742369 MS.gene068040:intron 35.0%
ATGCAGCTTCTAGTATATAG+TGG + chr4.3:20744444-20744463 MS.gene068040:intron 35.0%
ATGGCAAACATGTTAAAGGT+AGG + chr4.3:20743516-20743535 MS.gene068040:intron 35.0%
ATTTGGACTTGAAGATCACT+TGG - chr4.3:20742168-20742187 None:intergenic 35.0%
CGACTTGAATAAGATAGCTA+TGG + chr4.3:20742696-20742715 MS.gene068040:CDS 35.0%
CTAATCCAAAAGGAATAAGG+TGG + chr4.3:20743022-20743041 MS.gene068040:CDS 35.0%
CTTGTTCAAGAACAAACTGA+AGG + chr4.3:20743775-20743794 MS.gene068040:CDS 35.0%
GAACAACTTGGTTTATAGGT+TGG - chr4.3:20742307-20742326 None:intergenic 35.0%
GAGAATGGCAAACATGTTAA+AGG + chr4.3:20743512-20743531 MS.gene068040:CDS 35.0%
GCTTCTAGTATATAGTGGTA+TGG + chr4.3:20744449-20744468 MS.gene068040:intron 35.0%
GGACTTCATATGATTGTTTC+TGG + chr4.3:20743369-20743388 MS.gene068040:CDS 35.0%
GGTATGAGATTTGAACTGAT+TGG + chr4.3:20743210-20743229 MS.gene068040:CDS 35.0%
GTGCTAATCCAAAAGGAATA+AGG + chr4.3:20743019-20743038 MS.gene068040:CDS 35.0%
TAGCTATCTTATTCAAGTCG+GGG - chr4.3:20742696-20742715 None:intergenic 35.0%
TATACTAGAAGCTGCATTCA+CGG - chr4.3:20744441-20744460 None:intergenic 35.0%
TCAAACCAAGGATCAAAAGA+TGG + chr4.3:20743416-20743435 MS.gene068040:CDS 35.0%
TCAGAAAAGGATTGTCTACT+TGG - chr4.3:20743808-20743827 None:intergenic 35.0%
TGAGTATCTTGAAATGACTG+AGG + chr4.3:20742825-20742844 MS.gene068040:CDS 35.0%
TGAGTGTATGTTTGGTATCA+TGG - chr4.3:20742403-20742422 None:intergenic 35.0%
TGTATGTTTGGTATCATGGT+CGG - chr4.3:20742399-20742418 None:intergenic 35.0%
TTTCCACTTCTAGATAGCAA+AGG - chr4.3:20742643-20742662 None:intergenic 35.0%
! ACAGGAAAACACTTCTAGAA+GGG + chr4.3:20744921-20744940 MS.gene068040:CDS 35.0%
! ACTGATTTAGAGAAAAGGGT+TGG + chr4.3:20743646-20743665 MS.gene068040:CDS 35.0%
! ATTTGAAGATTTTCTGCCCA+CGG - chr4.3:20742961-20742980 None:intergenic 35.0%
! CTTCTAGTTGTCTTTCTGTT+CGG - chr4.3:20745196-20745215 None:intergenic 35.0%
! TATCCTTTGCTATCTAGAAG+TGG + chr4.3:20742637-20742656 MS.gene068040:CDS 35.0%
! TTTGGAGCTTCAACATGATA+TGG + chr4.3:20745033-20745052 MS.gene068040:CDS 35.0%
! TTTTCTGATCACAAGCATGT+GGG + chr4.3:20743820-20743839 MS.gene068040:CDS 35.0%
!! GTTTCACTTTTCTCAAAGCA+AGG - chr4.3:20743712-20743731 None:intergenic 35.0%
!! GTTTTCCTGTTTGTTACCAT+TGG - chr4.3:20744911-20744930 None:intergenic 35.0%
!!! GCTATGTTGTTTTGTCTTCA+TGG + chr4.3:20742893-20742912 MS.gene068040:CDS 35.0%
AAGGTAGGCTGTGTTAGATA+TGG + chr4.3:20743531-20743550 MS.gene068040:intron 40.0%
ACAGAAAGACAACTAGAAGG+TGG + chr4.3:20745197-20745216 MS.gene068040:CDS 40.0%
ACATCTTCAAACCACCAATC+AGG - chr4.3:20743135-20743154 None:intergenic 40.0%
ACCAACCAATGGTAACAAAC+AGG + chr4.3:20744903-20744922 MS.gene068040:CDS 40.0%
ACTTGCACTACTACCCTTAA+TGG - chr4.3:20744809-20744828 None:intergenic 40.0%
AGATTTGAACTGATTGGTGC+TGG + chr4.3:20743216-20743235 MS.gene068040:CDS 40.0%
AGCAACTCCATAGCAGAATT+TGG - chr4.3:20742761-20742780 None:intergenic 40.0%
AGCGGAACAACTTGGTTTAT+AGG - chr4.3:20742311-20742330 None:intergenic 40.0%
ATCCTGACTGGAACAATCTT+TGG + chr4.3:20742459-20742478 MS.gene068040:intron 40.0%
ATGACTGAGGATCTAGAAGA+AGG + chr4.3:20742838-20742857 MS.gene068040:CDS 40.0%
ATGATGTGGATCTTGTTCAG+AGG + chr4.3:20743737-20743756 MS.gene068040:CDS 40.0%
ATTATAAGGACCTGGCAATC+CGG - chr4.3:20742514-20742533 None:intergenic 40.0%
CAACACTAACTGAGCATGAA+AGG + chr4.3:20744705-20744724 MS.gene068040:CDS 40.0%
CACATGCTTGTGATCAGAAA+AGG - chr4.3:20743821-20743840 None:intergenic 40.0%
CATCCTTCTTGGAAAGATTG+CGG - chr4.3:20744950-20744969 None:intergenic 40.0%
CCTGAAACTTTGTCGATGAA+AGG - chr4.3:20743921-20743940 None:intergenic 40.0%
CCTTTCATCGACAAAGTTTC+AGG + chr4.3:20743918-20743937 MS.gene068040:CDS 40.0%
CGAACAGAAAGACAACTAGA+AGG + chr4.3:20745194-20745213 MS.gene068040:CDS 40.0%
CTGTTCGGTTCTTCAGAAAT+TGG - chr4.3:20745181-20745200 None:intergenic 40.0%
CTTAACACCATGTTTGGTTG+AGG - chr4.3:20741959-20741978 None:intergenic 40.0%
CTTTCATGCTCAGTTAGTGT+TGG - chr4.3:20744707-20744726 None:intergenic 40.0%
GAAAAGATACCTTGTGCAAG+TGG - chr4.3:20742206-20742225 None:intergenic 40.0%
TATGCAACTAAATGGCTACC+AGG + chr4.3:20743249-20743268 MS.gene068040:CDS 40.0%
TGAATCTCAGTACCAACCAA+TGG + chr4.3:20744892-20744911 MS.gene068040:CDS 40.0%
TGTGGACAGTTATCTTACAG+AGG + chr4.3:20743879-20743898 MS.gene068040:CDS 40.0%
TTCATCAAAGAGGCAACTCA+TGG + chr4.3:20741989-20742008 MS.gene068040:CDS 40.0%
TTGACAGCAAGCAACATTTC+CGG + chr4.3:20742787-20742806 MS.gene068040:CDS 40.0%
! ATTTCCTCTCTTTTGCATGC+AGG + chr4.3:20742124-20742143 MS.gene068040:intron 40.0%
! CTTTTCTGATCACAAGCATG+TGG + chr4.3:20743819-20743838 MS.gene068040:CDS 40.0%
! TCCTGTTTGTTACCATTGGT+TGG - chr4.3:20744907-20744926 None:intergenic 40.0%
!! TGGTCGGTTTGTTAGAATCA+CGG - chr4.3:20742383-20742402 None:intergenic 40.0%
AAAGTGAAACAGCAGCTGGA+AGG + chr4.3:20741911-20741930 MS.gene068040:CDS 45.0%
AACTAAATGGCTACCAGGAC+TGG + chr4.3:20743254-20743273 MS.gene068040:CDS 45.0%
AAGTGAAACAGCAGCTGGAA+GGG + chr4.3:20741912-20741931 MS.gene068040:CDS 45.0%
ACAACTTGAGCGGAACAACT+TGG - chr4.3:20742319-20742338 None:intergenic 45.0%
ACACCGCAATCTTTCCAAGA+AGG + chr4.3:20744944-20744963 MS.gene068040:CDS 45.0%
AGACTTTACTTGTCTCCTGG+CGG + chr4.3:20742484-20742503 MS.gene068040:intron 45.0%
ATAGCTATGGATGATCTCCC+TGG + chr4.3:20742709-20742728 MS.gene068040:CDS 45.0%
CATACCTGCATGCAAAAGAG+AGG - chr4.3:20742131-20742150 None:intergenic 45.0%
CGAAAGCTTCAAGCCCAAAA+TGG + chr4.3:20743064-20743083 MS.gene068040:CDS 45.0%
CGCAATCTTTCCAAGAAGGA+TGG + chr4.3:20744948-20744967 MS.gene068040:CDS 45.0%
CTAGCCAACAGCTCTCTAAA+AGG + chr4.3:20744863-20744882 MS.gene068040:CDS 45.0%
CTATAGACTCGCTGCATCTT+CGG - chr4.3:20742987-20743006 None:intergenic 45.0%
CTTGCAGCCAAATTCTGCTA+TGG + chr4.3:20742751-20742770 MS.gene068040:CDS 45.0%
GATACTCAGCAGCACATCTT+AGG - chr4.3:20742813-20742832 None:intergenic 45.0%
GCTGCAAGTTCAAATGCTTC+AGG - chr4.3:20742739-20742758 None:intergenic 45.0%
GGATCTTGTTCAGAGGTTGT+TGG + chr4.3:20743744-20743763 MS.gene068040:CDS 45.0%
GTTCTCACCTCAACCAAACA+TGG + chr4.3:20741949-20741968 MS.gene068040:CDS 45.0%
TAGCTGTTGTCCATCCTTCT+TGG - chr4.3:20744961-20744980 None:intergenic 45.0%
TCAGAAAGTGAAACAGCAGC+TGG + chr4.3:20741907-20741926 MS.gene068040:CDS 45.0%
TCCAGTCAGGATTCGAACTT+GGG - chr4.3:20742451-20742470 None:intergenic 45.0%
TCTTCAAACCACCAATCAGG+AGG - chr4.3:20743132-20743151 None:intergenic 45.0%
TGCAGAAGCTTTACCTGAGT+CGG + chr4.3:20743945-20743964 MS.gene068040:CDS 45.0%
TTCCAGTCAGGATTCGAACT+TGG - chr4.3:20742452-20742471 None:intergenic 45.0%
! AGCAAAAGCACACATCAGCT+TGG + chr4.3:20745089-20745108 MS.gene068040:CDS 45.0%
! TACGCCCACCTTATTCCTTT+TGG - chr4.3:20743030-20743049 None:intergenic 45.0%
! TGTTGCTTGTTTCCTCTCCT+TGG - chr4.3:20743299-20743318 None:intergenic 45.0%
!! ACACATCAGCTTGGACTAGT+GGG + chr4.3:20745098-20745117 MS.gene068040:CDS 45.0%
!! CGGTTTGTTAGAATCACGGT+GGG - chr4.3:20742379-20742398 None:intergenic 45.0%
!! TCGGTTTGTTAGAATCACGG+TGG - chr4.3:20742380-20742399 None:intergenic 45.0%
AACAACAGTAGCTGGAGAGG+TGG + chr4.3:20743348-20743367 MS.gene068040:CDS 50.0%
ACAGCAGCTGGAAGGGAATA+TGG + chr4.3:20741919-20741938 MS.gene068040:CDS 50.0%
ACCCAAGTTCGAATCCTGAC+TGG + chr4.3:20742447-20742466 MS.gene068040:intron 50.0%
AGCAGCTGGAAGGGAATATG+GGG + chr4.3:20741921-20741940 MS.gene068040:CDS 50.0%
ATCAGCTAGTGCCTCCTGAT+TGG + chr4.3:20743118-20743137 MS.gene068040:CDS 50.0%
CACGAGACACTGTCTTTCTG+CGG - chr4.3:20743471-20743490 None:intergenic 50.0%
CAGCAGCTGGAAGGGAATAT+GGG + chr4.3:20741920-20741939 MS.gene068040:CDS 50.0%
CCATTCTCAGAAGCCTGAGA+AGG - chr4.3:20743500-20743519 None:intergenic 50.0%
CGACACTACAATCCAAGGAG+AGG + chr4.3:20743284-20743303 MS.gene068040:CDS 50.0%
CTATGGATGATCTCCCTGGA+GGG + chr4.3:20742713-20742732 MS.gene068040:CDS 50.0%
GACAACAACAGTAGCTGGAG+AGG + chr4.3:20743345-20743364 MS.gene068040:CDS 50.0%
GATGCAGCGAGTCTATAGCT+TGG + chr4.3:20742989-20743008 MS.gene068040:CDS 50.0%
GCCAGACTTTACTTGTCTCC+TGG + chr4.3:20742481-20742500 MS.gene068040:intron 50.0%
GCCAGGAGACAAGTAAAGTC+TGG - chr4.3:20742485-20742504 None:intergenic 50.0%
GCTGCATTCACGGTAGAATC+AGG - chr4.3:20744431-20744450 None:intergenic 50.0%
GGAATAAGGTGGGCGTATAC+CGG + chr4.3:20743033-20743052 MS.gene068040:CDS 50.0%
GGCCAAAGATTGTTCCAGTC+AGG - chr4.3:20742464-20742483 None:intergenic 50.0%
GGGAACGACACTACAATCCA+AGG + chr4.3:20743279-20743298 MS.gene068040:CDS 50.0%
TAAATGGCTACCAGGACTGG+CGG + chr4.3:20743257-20743276 MS.gene068040:CDS 50.0%
TACCTTGTGCAAGTGGAAGC+TGG - chr4.3:20742199-20742218 None:intergenic 50.0%
TCGGCTAGAGTTTCTGACGA+TGG + chr4.3:20743964-20743983 MS.gene068040:CDS 50.0%
TCTCGTGTAGCTTCCTTCTC+AGG + chr4.3:20743484-20743503 MS.gene068040:CDS 50.0%
TCTGATCACAAGCATGTGGG+AGG + chr4.3:20743823-20743842 MS.gene068040:CDS 50.0%
TGAAGCTTTCGTCGCTCTTC+CGG - chr4.3:20743055-20743074 None:intergenic 50.0%
! ATTTTGAGCTGCGTGCATGC+AGG - chr4.3:20744777-20744796 None:intergenic 50.0%
! CACACATCAGCTTGGACTAG+TGG + chr4.3:20745097-20745116 MS.gene068040:CDS 50.0%
! CCTTCTCAGGCTTCTGAGAA+TGG + chr4.3:20743497-20743516 MS.gene068040:CDS 50.0%
! CTCTGAAAAGCTGTCACCGT+GGG + chr4.3:20742942-20742961 MS.gene068040:CDS 50.0%
! GAAGCGTCTCTGTCGTGTAA+TGG + chr4.3:20744727-20744746 MS.gene068040:CDS 50.0%
! GGAAGCTGGCTTCTCCAATT+TGG - chr4.3:20742185-20742204 None:intergenic 50.0%
! TCAGAAACTCTAGCCGACTC+AGG - chr4.3:20743961-20743980 None:intergenic 50.0%
!! GCTGCCTTTTAGAGAGCTGT+TGG - chr4.3:20744870-20744889 None:intergenic 50.0%
AAGCCTTGCCACTCTTGCCT+TGG - chr4.3:20743860-20743879 None:intergenic 55.0%
AGCCAGCTTCCACTTGCACA+AGG + chr4.3:20742194-20742213 MS.gene068040:CDS 55.0%
AGCTAGTGCCTCCTGATTGG+TGG + chr4.3:20743121-20743140 MS.gene068040:CDS 55.0%
ATGCCAAGGCAAGAGTGGCA+AGG + chr4.3:20743854-20743873 MS.gene068040:CDS 55.0%
CAAATGCTTCAGGCCCTCCA+GGG - chr4.3:20742729-20742748 None:intergenic 55.0%
CCACTCTTGCCTTGGCATTC+AGG - chr4.3:20743852-20743871 None:intergenic 55.0%
CCTGAATGCCAAGGCAAGAG+TGG + chr4.3:20743849-20743868 MS.gene068040:CDS 55.0%
CGGGGTCGCGAGAATCATAT+AGG - chr4.3:20742678-20742697 None:intergenic 55.0%
CTCAGCAGCACATCTTAGGC+CGG - chr4.3:20742809-20742828 None:intergenic 55.0%
GAGACACTGTCTTTCTGCGG+CGG - chr4.3:20743468-20743487 None:intergenic 55.0%
GCAGCTGGAAGGGAATATGG+GGG + chr4.3:20741922-20741941 MS.gene068040:CDS 55.0%
GCGGTGACAACAACAGTAGC+TGG + chr4.3:20743340-20743359 MS.gene068040:CDS 55.0%
GCTATGGATGATCTCCCTGG+AGG + chr4.3:20742712-20742731 MS.gene068040:CDS 55.0%
GGGAGGTAACCTGAATGCCA+AGG + chr4.3:20743840-20743859 MS.gene068040:CDS 55.0%
TCAAATGCTTCAGGCCCTCC+AGG - chr4.3:20742730-20742749 None:intergenic 55.0%
TGAAAGACTCAAGCCCGAGC+AGG + chr4.3:20743094-20743113 MS.gene068040:CDS 55.0%
! AAATGGCTACCAGGACTGGC+GGG + chr4.3:20743258-20743277 MS.gene068040:CDS 55.0%
! GCACTAGCTGATTCCTGCTC+GGG - chr4.3:20743110-20743129 None:intergenic 55.0%
! GGCACTAGCTGATTCCTGCT+CGG - chr4.3:20743111-20743130 None:intergenic 55.0%
!! CATCAGCTTGGACTAGTGGG+TGG + chr4.3:20745101-20745120 MS.gene068040:CDS 55.0%
!! GCTCTGAAAAGCTGTCACCG+TGG + chr4.3:20742941-20742960 MS.gene068040:CDS 55.0%
CTTGTCTCCTGGCGGAACTC+CGG + chr4.3:20742492-20742511 MS.gene068040:intron 60.0%
GCTGGAAGGGAATATGGGGG+TGG + chr4.3:20741925-20741944 MS.gene068040:CDS 60.0%
GGCAAGAGTGGCAAGGCTTG+TGG + chr4.3:20743861-20743880 MS.gene068040:CDS 60.0%
TGCGTGCATGCAGGCGTCAA+TGG - chr4.3:20744768-20744787 None:intergenic 60.0%
! AATGGCTACCAGGACTGGCG+GGG + chr4.3:20743259-20743278 MS.gene068040:CDS 60.0%
TGGCAATCCGGAGTTCCGCC+AGG - chr4.3:20742502-20742521 None:intergenic 65.0%
TGGCGGAACTCCGGATTGCC+AGG + chr4.3:20742501-20742520 MS.gene068040:intron 65.0%
! GTGTCGTTCCCCGCCAGTCC+TGG - chr4.3:20743270-20743289 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 20741898 20745237 20741898 ID=MS.gene068040
chr4.3 mRNA 20741898 20745237 20741898 ID=MS.gene068040.t1;Parent=MS.gene068040
chr4.3 exon 20741898 20742010 20741898 ID=MS.gene068040.t1.exon1;Parent=MS.gene068040.t1
chr4.3 CDS 20741898 20742010 20741898 ID=cds.MS.gene068040.t1;Parent=MS.gene068040.t1
chr4.3 exon 20742146 20742215 20742146 ID=MS.gene068040.t1.exon2;Parent=MS.gene068040.t1
chr4.3 CDS 20742146 20742215 20742146 ID=cds.MS.gene068040.t1;Parent=MS.gene068040.t1
chr4.3 exon 20742637 20743533 20742637 ID=MS.gene068040.t1.exon3;Parent=MS.gene068040.t1
chr4.3 CDS 20742637 20743533 20742637 ID=cds.MS.gene068040.t1;Parent=MS.gene068040.t1
chr4.3 exon 20743628 20744017 20743628 ID=MS.gene068040.t1.exon4;Parent=MS.gene068040.t1
chr4.3 CDS 20743628 20744017 20743628 ID=cds.MS.gene068040.t1;Parent=MS.gene068040.t1
chr4.3 exon 20744698 20745237 20744698 ID=MS.gene068040.t1.exon5;Parent=MS.gene068040.t1
chr4.3 CDS 20744698 20745237 20744698 ID=cds.MS.gene068040.t1;Parent=MS.gene068040.t1
Gene Sequence

>MS.gene068040

ATGTGGGAATCAGAAAGTGAAACAGCAGCTGGAAGGGAATATGGGGGTGGAGTTCTCACCTCAACCAAACATGGTGTTAAGATTGAAGGTTTTCATCAAAGAGGCAACTCATGGTATGTTAATGTTTAATTCAATGCAGAAAATCATTATTGAAACTTCTAAAATGATCATAATATTTTCTTTTTTTGAACTTTTTATTTATCCATTTTATGTAAGTTAATTGAACATTTCCTCTCTTTTGCATGCAGGTATGTTTCAGCTGATATTCCAAGTGATCTTCAAGTCCAAATTGGAGAAGCCAGCTTCCACTTGCACAAGGTATCTTTTCTTTTCATATTTCATGTAATTTTTTTTTCTTTCCTAGAAAGTTATTTTATTTTTTCTCTAAGGATGATAAGATATACTGCCAACCTATAAACCAAGTTGTTCCGCTCAAGTTGTTAATGAGTTTCAGTGATGTTTGGTATCAAGTTGGACTCCCACCGTGATTCTAACAAACCGACCATGATACCAAACATACACTCAATTAAGGATGATTCATTTGGGAGAACCCAAGTTCGAATCCTGACTGGAACAATCTTTGGCCAGACTTTACTTGTCTCCTGGCGGAACTCCGGATTGCCAGGTCCTTATAATCTATAAACCGAAAGGTTAAGAAAAAAACTACTACTGATCTGTACTGTATTTGAACTGTATTGTGTTGAATTTGATGAACTTTTCTTCCTTTTTGTTGTGAAAGTATCCTTTGCTATCTAGAAGTGGAAAGCTGAACAGAATCCTATATGATTCTCGCGACCCCGACTTGAATAAGATAGCTATGGATGATCTCCCTGGAGGGCCTGAAGCATTTGAACTTGCAGCCAAATTCTGCTATGGAGTTGCTATTGATTTGACAGCAAGCAACATTTCCGGCCTAAGATGTGCTGCTGAGTATCTTGAAATGACTGAGGATCTAGAAGAAGGAAATCTTATATTCAAGACTGAAGCTTTTCTTAGCTATGTTGTTTTGTCTTCATGGAGAGACTCTATAGTAGTGTTGAAAAGCTCTGAAAAGCTGTCACCGTGGGCAGAAAATCTTCAAATTGTCCGAAGATGCAGCGAGTCTATAGCTTGGAAAGCTTGTGCTAATCCAAAAGGAATAAGGTGGGCGTATACCGGAAGAGCGACGAAAGCTTCAAGCCCAAAATGGAACGAAATGAAAGACTCAAGCCCGAGCAGGAATCAGCTAGTGCCTCCTGATTGGTGGTTTGAAGATGTTTCAATCCTTAGGATTGATCACTTTGTTAGAGTCATTACTGCTATTAAGGTAAAAGGTATGAGATTTGAACTGATTGGTGCTGGAATAATGCATTATGCAACTAAATGGCTACCAGGACTGGCGGGGAACGACACTACAATCCAAGGAGAGGAAACAAGCAACAACAGTAATAGTAGTTTTAGTAGCGGTGACAACAACAGTAGCTGGAGAGGTGGACTTCATATGATTGTTTCTGGAACTAGAGATGAAACTTCAAGTCTTCAAACCAAGGATCAAAAGATGGTTATTGAGAGTCTCATCAGCATAATTCCGCCGCAGAAAGACAGTGTCTCGTGTAGCTTCCTTCTCAGGCTTCTGAGAATGGCAAACATGTTAAAGGTAGGCTGTGTTAGATATGGCAACCATAACTAACTTGTAACTACCTTAAACAATGTCTAACTAATTCTAACAAATATCAAACTATCTCTAACAGGTAGCATATGCATTGATTACTGATTTAGAGAAAAGGGTTGGAATGCAGTTTGAGCAAGCTACACTTTCTGATCTTCTAATTCCTTGCTTTGAGAAAAGTGAAACTATGTATGATGTGGATCTTGTTCAGAGGTTGTTGGAGCATTTTCTTGTTCAAGAACAAACTGAAGGTTATAGTCCAAGTAGACAATCCTTTTCTGATCACAAGCATGTGGGAGGTAACCTGAATGCCAAGGCAAGAGTGGCAAGGCTTGTGGACAGTTATCTTACAGAGGTATCAAGAGATAGAAACCTTTCATCGACAAAGTTTCAGGTTCTTGCAGAAGCTTTACCTGAGTCGGCTAGAGTTTCTGACGATGGACTATATAGAGCAATTGATTCATATCTCAAGGTAATTAATCACTTTTCAAAGGATAAAGGGGAACAATCTTTCAAGTTTCTTATCATTTTTTATTTTCAATTACTCTAGGATACATGAACTTTAAGTAAAATTTGAGTCAGAACTTGGAGTTGCATAATATGCTCATTATACACATCTTAAAAGCAAAGTTGTATTGGTTTTGGCATCTTTGTTTAAGAAATGTGCATATATGCACATTCTGAAATATGACAATATACTTATTTAGGTTCTATAATTAATTGAACACTGAATTTATGTACTTTATGTGCTAATATTTCAAGATAACATTGGAGGAAGCGCTATTATATCCCTTCATCGCAATTATATCTTTACGACAAGATTACAAAAGCCTCAAATTTCATTTTCAATGAGATTACATTTGACTTACAGAATAAACTTTTCCTGATTCTACCGTGAATGCAGCTTCTAGTATATAGTGGTATGGTATTTACAAAAGATATGAATTTAGTAAACACATAATCAATCATTTCTCTAGAAAAGATTAGCTATATTTGTCATTGTTTGAAATAATAAATGCACCAATTTCATTGATGTCTTATGTACTCATTTTAGAAGAATGAGTCGGAACAATTAGTATAGTCTAGATTCACATGATATGATCATGGATTTATATTTCTTTACTTCTGATGATCTGTGAAACAAATTAACAGGCACATCCAACACTAACTGAGCATGAAAGGAAGCGTCTCTGTCGTGTAATGGATTGTCAAAAACTCTCCATTGACGCCTGCATGCACGCAGCTCAAAATGAACGACTGCCATTAAGGGTAGTAGTGCAAGTTCTATTCTCTGAACAAGTAAAGATAAGCAACGCACTAGCCAACAGCTCTCTAAAAGGCAGCGTTGAATCTCAGTACCAACCAATGGTAACAAACAGGAAAACACTTCTAGAAGGGACACCGCAATCTTTCCAAGAAGGATGGACAACAGCTAAGAAAGACATTAACACATTAAAGTTTGAACTTGAGAGTGTGAAGACAAAATATTTGGAGCTTCAACATGATATGGAAAATCTGCAGAAAAATTTCGATAAGCTGTTGAAGCAAAAGCACACATCAGCTTGGACTAGTGGGTGGAAGAAACTGAGCAAACTTACTAAAATGACAAATGTGGAAAATCATGATATGGTTCCAATTTCTGAAGAACCGAACAGAAAGACAACTAGAAGGTGGAGAAACTCAATATCTTGA
Protein sequence

>MS.gene068040.t1

MWESESETAAGREYGGGVLTSTKHGVKIEGFHQRGNSWYVSADIPSDLQVQIGEASFHLHKYPLLSRSGKLNRILYDSRDPDLNKIAMDDLPGGPEAFELAAKFCYGVAIDLTASNISGLRCAAEYLEMTEDLEEGNLIFKTEAFLSYVVLSSWRDSIVVLKSSEKLSPWAENLQIVRRCSESIAWKACANPKGIRWAYTGRATKASSPKWNEMKDSSPSRNQLVPPDWWFEDVSILRIDHFVRVITAIKVKGMRFELIGAGIMHYATKWLPGLAGNDTTIQGEETSNNSNSSFSSGDNNSSWRGGLHMIVSGTRDETSSLQTKDQKMVIESLISIIPPQKDSVSCSFLLRLLRMANMLKVAYALITDLEKRVGMQFEQATLSDLLIPCFEKSETMYDVDLVQRLLEHFLVQEQTEGYSPSRQSFSDHKHVGGNLNAKARVARLVDSYLTEVSRDRNLSSTKFQVLAEALPESARVSDDGLYRAIDSYLKAHPTLTEHERKRLCRVMDCQKLSIDACMHAAQNERLPLRVVVQVLFSEQVKISNALANSSLKGSVESQYQPMVTNRKTLLEGTPQSFQEGWTTAKKDINTLKFELESVKTKYLELQHDMENLQKNFDKLLKQKHTSAWTSGWKKLSKLTKMTNVENHDMVPISEEPNRKTTRRWRNSIS