AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028462

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028462.t1 MTR_4g093850 99.103 669 6 0 1 669 1 669 0.0 1383
MS.gene028462.t1 MTR_2g009340 80.204 687 116 9 1 669 1 685 0.0 1097
MS.gene028462.t1 MTR_4g093850 97.200 500 14 0 1 500 1 500 0.0 1013
MS.gene028462.t1 MTR_8g090220 42.573 653 274 11 36 657 44 626 1.06e-172 508
MS.gene028462.t1 MTR_8g090220 46.388 526 208 10 36 543 44 513 7.56e-161 473
MS.gene028462.t1 MTR_2g084595 37.645 603 336 9 27 621 6 576 1.35e-139 422
MS.gene028462.t1 MTR_2g018750 39.512 615 316 13 19 625 1 567 4.85e-136 412
MS.gene028462.t1 MTR_5g015790 39.078 586 303 12 23 599 5 545 3.81e-134 408
MS.gene028462.t1 MTR_8g078900 36.124 645 332 12 34 633 10 619 3.50e-133 407
MS.gene028462.t1 MTR_5g016060 38.014 584 291 9 33 606 29 551 2.51e-129 395
MS.gene028462.t1 MTR_1g094850 38.179 626 327 14 19 635 1 575 7.61e-129 394
MS.gene028462.t1 MTR_5g032780 40.293 546 276 9 23 545 5 523 1.70e-128 394
MS.gene028462.t1 MTR_8g078900 36.437 623 316 12 56 633 2 589 1.04e-125 386
MS.gene028462.t1 MTR_4g022330 36.437 623 317 11 33 639 24 583 1.92e-125 385
MS.gene028462.t1 MTR_1g082690 35.871 591 287 10 26 610 40 544 4.10e-122 374
MS.gene028462.t1 MTR_1g014070 39.197 523 273 8 23 540 5 487 9.87e-122 375
MS.gene028462.t1 MTR_8g078900 37.696 573 297 11 86 633 42 579 9.21e-121 373
MS.gene028462.t1 MTR_8g069330 40.625 544 242 11 23 545 5 488 1.94e-120 372
MS.gene028462.t1 MTR_1g106430 38.015 534 284 9 23 550 5 497 3.10e-120 372
MS.gene028462.t1 MTR_1g064360 37.803 537 268 8 23 548 5 486 3.51e-120 370
MS.gene028462.t1 MTR_2g048390 41.962 479 244 7 19 490 1 452 7.17e-119 363
MS.gene028462.t1 MTR_4g095430 36.348 586 311 9 33 615 2 528 2.77e-117 363
MS.gene028462.t1 MTR_1g088885 32.735 669 371 13 20 669 13 621 4.53e-113 353
MS.gene028462.t1 MTR_4g134210 37.640 534 275 10 23 552 5 484 5.04e-113 352
MS.gene028462.t1 MTR_7g073980 34.634 615 331 15 40 643 29 583 5.06e-112 351
MS.gene028462.t1 MTR_2g027410 36.773 533 273 8 23 552 5 476 9.38e-108 338
MS.gene028462.t1 MTR_2g084595 38.004 471 256 7 27 491 6 446 1.26e-106 331
MS.gene028462.t1 MTR_7g108290 33.762 622 341 14 33 636 26 594 2.03e-106 337
MS.gene028462.t1 MTR_3g062540 32.843 612 324 12 33 640 13 541 3.43e-98 313
MS.gene028462.t1 MTR_8g078900 37.166 487 243 9 34 490 10 463 4.69e-98 309
MS.gene028462.t1 MTR_5g075640 34.375 512 280 9 33 542 14 471 2.76e-97 311
MS.gene028462.t1 MTR_2g027410 38.065 465 224 8 91 552 1 404 3.76e-93 297
MS.gene028462.t1 MTR_5g016060 36.016 497 247 9 120 606 2 437 3.39e-92 295
MS.gene028462.t1 MTR_3g062540 32.358 581 306 12 64 640 2 499 1.28e-86 281
MS.gene028462.t1 MTR_8g078900 39.518 415 208 8 86 490 42 423 7.46e-86 276
MS.gene028462.t1 MTR_5g075640 33.472 481 264 9 64 542 2 428 6.09e-85 277
MS.gene028462.t1 MTR_2g102730 36.111 468 231 11 216 669 10 423 2.69e-79 259
MS.gene028462.t1 MTR_4g011530 36.407 423 206 9 207 623 26 391 7.65e-79 258
MS.gene028462.t1 MTR_1g035720 29.379 531 258 16 47 539 6 457 5.72e-52 188
MS.gene028462.t1 MTR_4g074190 27.768 587 324 16 46 606 5 517 7.48e-52 188
MS.gene028462.t1 MTR_4g074190 27.440 543 298 12 86 606 3 471 3.32e-49 180
MS.gene028462.t1 MTR_4g045767 27.127 623 339 19 47 639 7 544 8.09e-49 180
MS.gene028462.t1 MTR_4g074190 27.061 473 263 12 46 494 5 419 6.56e-38 147
MS.gene028462.t1 MTR_1g105870 35.931 231 117 4 51 279 38 239 8.55e-35 133
MS.gene028462.t1 MTR_2g010890 24.724 453 276 6 110 559 1 391 3.06e-31 127
MS.gene028462.t1 MTR_2g048375 37.879 132 72 2 507 635 1 125 5.23e-21 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028462.t1 AT5G64330 70.139 720 144 12 1 649 2 721 0.0 962
MS.gene028462.t1 AT5G64330 70.433 646 123 11 1 578 2 647 0.0 863
MS.gene028462.t1 AT5G64330 71.012 583 108 9 57 578 6 588 0.0 786
MS.gene028462.t1 AT5G10250 38.838 654 293 12 10 637 16 588 8.61e-152 453
MS.gene028462.t1 AT1G30440 38.931 655 355 11 19 654 1 629 2.08e-151 454
MS.gene028462.t1 AT5G10250 38.880 643 293 12 10 637 16 573 3.26e-148 444
MS.gene028462.t1 AT5G03250 41.682 535 267 8 25 547 7 508 2.05e-136 413
MS.gene028462.t1 AT5G13600 39.315 613 306 17 19 623 1 555 4.58e-130 396
MS.gene028462.t1 AT3G44820 36.394 599 353 6 27 618 6 583 7.82e-130 398
MS.gene028462.t1 AT2G14820 38.590 539 274 9 23 550 5 497 3.95e-124 382
MS.gene028462.t1 AT2G14820 38.590 539 274 9 23 550 5 497 3.95e-124 382
MS.gene028462.t1 AT5G67440 40.190 525 255 9 23 541 5 476 4.40e-124 380
MS.gene028462.t1 AT5G67440 40.190 525 255 9 23 541 5 476 4.40e-124 380
MS.gene028462.t1 AT1G67900 39.855 552 266 8 23 548 5 516 4.42e-122 377
MS.gene028462.t1 AT1G67900 39.855 552 266 8 23 548 5 516 4.42e-122 377
MS.gene028462.t1 AT1G67900 39.855 552 266 8 23 548 5 516 4.42e-122 377
MS.gene028462.t1 AT1G67900 39.855 552 266 8 23 548 5 516 4.42e-122 377
MS.gene028462.t1 AT1G67900 39.855 552 266 8 23 548 5 516 4.42e-122 377
MS.gene028462.t1 AT1G67900 39.855 552 266 8 23 548 5 516 4.42e-122 377
MS.gene028462.t1 AT5G67385 39.847 522 258 8 33 550 14 483 1.28e-121 375
MS.gene028462.t1 AT4G37590 37.879 594 269 15 23 599 5 515 7.85e-116 359
MS.gene028462.t1 AT5G48800 35.655 603 329 7 37 636 34 580 2.86e-115 358
MS.gene028462.t1 AT5G67440 40.574 488 232 8 59 541 5 439 4.38e-115 356
MS.gene028462.t1 AT5G66560 33.828 674 319 15 34 630 9 632 7.75e-115 359
MS.gene028462.t1 AT4G31820 37.477 547 269 12 23 562 5 485 1.08e-114 356
MS.gene028462.t1 AT1G03010 33.385 641 346 14 23 638 16 600 1.14e-111 350
MS.gene028462.t1 AT3G08570 35.033 608 327 11 40 640 30 576 1.27e-111 349
MS.gene028462.t1 AT1G03010 33.762 622 332 13 42 638 7 573 8.46e-111 347
MS.gene028462.t1 AT1G03010 33.762 622 332 13 42 638 33 599 2.05e-110 347
MS.gene028462.t1 AT3G26490 37.004 554 267 12 25 545 7 511 3.23e-108 340
MS.gene028462.t1 AT3G26490 37.004 554 267 12 25 545 7 511 3.23e-108 340
MS.gene028462.t1 AT3G26490 37.004 554 267 12 25 545 7 511 3.23e-108 340
MS.gene028462.t1 AT5G47800 37.094 523 270 9 23 539 5 474 3.44e-108 338
MS.gene028462.t1 AT5G47800 37.094 523 270 9 23 539 5 474 3.44e-108 338
MS.gene028462.t1 AT5G47800 37.094 523 270 9 23 539 5 474 3.44e-108 338
MS.gene028462.t1 AT2G47860 34.667 600 314 10 38 618 32 572 3.84e-108 341
MS.gene028462.t1 AT5G67385 39.388 490 241 9 65 550 1 438 7.83e-108 338
MS.gene028462.t1 AT5G67385 39.388 490 241 9 65 550 1 438 7.83e-108 338
MS.gene028462.t1 AT5G67385 39.388 490 241 9 65 550 1 438 7.83e-108 338
MS.gene028462.t1 AT5G67385 39.388 490 241 9 65 550 1 438 7.83e-108 338
MS.gene028462.t1 AT2G30520 34.635 589 295 12 34 615 20 525 2.78e-107 337
MS.gene028462.t1 AT5G66560 34.202 652 302 15 56 630 2 603 3.11e-107 338
MS.gene028462.t1 AT3G08660 35.043 585 287 13 40 615 30 530 1.17e-106 335
MS.gene028462.t1 AT5G47800 37.094 523 270 9 23 539 118 587 1.25e-106 338
MS.gene028462.t1 AT5G66560 36.159 578 286 13 86 630 66 593 9.28e-104 329
MS.gene028462.t1 AT2G47860 33.387 623 314 11 38 618 32 595 6.14e-103 328
MS.gene028462.t1 AT4G31820 36.948 498 242 11 71 562 1 432 7.85e-99 313
MS.gene028462.t1 AT2G23050 35.000 540 255 12 23 553 5 457 9.82e-98 309
MS.gene028462.t1 AT3G08660 34.477 554 270 13 71 615 1 470 2.11e-95 304
MS.gene028462.t1 AT3G50840 33.608 607 318 14 38 633 10 542 2.43e-95 305
MS.gene028462.t1 AT2G30520 33.810 559 280 12 64 615 2 477 3.42e-94 301
MS.gene028462.t1 AT2G30520 33.810 559 280 12 64 615 2 477 3.42e-94 301
MS.gene028462.t1 AT5G03250 39.627 429 216 7 129 547 1 396 2.01e-92 295
MS.gene028462.t1 AT3G49970 33.874 555 257 11 56 608 6 452 1.42e-88 286
MS.gene028462.t1 AT3G49970 33.874 555 257 11 56 608 6 452 1.42e-88 286
MS.gene028462.t1 AT3G26490 34.800 500 244 12 25 491 7 457 2.60e-83 272
MS.gene028462.t1 AT3G50840 33.333 579 303 13 65 633 1 506 3.59e-83 272
MS.gene028462.t1 AT3G50840 33.333 579 303 13 65 633 1 506 3.59e-83 272
MS.gene028462.t1 AT5G66560 33.716 522 232 13 34 491 9 480 1.85e-81 266
MS.gene028462.t1 AT2G47860 35.381 472 228 9 38 491 32 444 1.61e-80 263
MS.gene028462.t1 AT1G52770 36.183 503 232 16 205 669 3 454 3.53e-80 262
MS.gene028462.t1 AT3G08660 33.978 465 230 9 40 498 30 423 3.36e-79 258
MS.gene028462.t1 AT3G15570 34.047 467 238 9 230 669 29 452 1.14e-76 253
MS.gene028462.t1 AT2G47860 33.737 495 228 10 38 491 32 467 2.01e-75 250
MS.gene028462.t1 AT2G47860 31.238 509 276 8 129 618 1 454 4.20e-69 234
MS.gene028462.t1 AT2G47860 31.238 509 276 8 129 618 1 454 4.20e-69 234
MS.gene028462.t1 AT2G23050 33.879 428 193 10 129 553 1 341 5.61e-65 219
MS.gene028462.t1 AT3G19850 28.409 528 281 9 44 540 3 464 3.92e-56 200
MS.gene028462.t1 AT1G50280 28.487 509 292 13 46 535 5 460 6.35e-49 179
MS.gene028462.t1 AT1G50280 29.374 463 261 11 86 535 18 427 8.38e-48 176
MS.gene028462.t1 AT5G17580 27.778 522 300 11 40 553 16 468 1.27e-43 165
MS.gene028462.t1 AT5G17580 27.778 522 300 11 40 553 1 453 1.28e-43 165
MS.gene028462.t1 AT3G22104 26.477 491 275 13 46 526 6 420 3.31e-39 152
MS.gene028462.t1 AT3G03510 27.660 470 235 13 94 556 4 375 1.68e-37 145
MS.gene028462.t1 AT5G48130 32.911 237 145 4 37 269 32 258 3.42e-34 139
MS.gene028462.t1 AT5G48130 29.767 215 121 5 424 614 353 561 1.06e-19 94.0
MS.gene028462.t1 AT3G22104 24.821 419 232 10 115 526 3 345 9.05e-26 111
MS.gene028462.t1 AT3G22104 24.821 419 232 10 115 526 3 345 9.05e-26 111
MS.gene028462.t1 AT3G22104 24.821 419 232 10 115 526 3 345 9.05e-26 111
MS.gene028462.t1 AT3G49900 33.000 200 107 7 34 221 55 239 5.55e-14 75.5
MS.gene028462.t1 AT3G49900 33.000 200 107 7 34 221 55 239 6.12e-14 75.5
MS.gene028462.t1 AT3G49900 33.981 103 63 3 445 546 214 312 4.46e-11 66.2

Find 140 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCATTTCGTTCCATTT+TGG 0.129719 4.1:-19880462 None:intergenic
CTGTTCGGTTCTTCAGAAAT+TGG 0.136199 4.1:-19882565 None:intergenic
GGACTTCATATGATTGTTTC+TGG 0.160796 4.1:+19880753 MS.gene028462:CDS
CCACTCTTGCCTTGGCATTC+AGG 0.181350 4.1:-19881233 None:intergenic
CCTTTCATCGACAAAGTTTC+AGG 0.183368 4.1:+19881302 MS.gene028462:CDS
GGAAACTCGCTTCTCCAATT+TGG 0.205887 4.1:-19879548 None:intergenic
ACAGCAGCTGGAAGGGAATA+TGG 0.207369 4.1:+19879285 MS.gene028462:CDS
TAGAGTTATTACTGCTATTA+AGG 0.220033 4.1:+19880566 MS.gene028462:CDS
ATTACTGCTATTAAGGTAAA+AGG 0.233214 4.1:+19880573 MS.gene028462:CDS
AAAATGAACGACTGCCATTA+AGG 0.241454 4.1:+19882179 MS.gene028462:CDS
TACGCCCACCTTATTCCTTT+TGG 0.245910 4.1:-19880411 None:intergenic
TAGCTGTTGTCCATCCTTCT+TGG 0.246421 4.1:-19882345 None:intergenic
ACTTGCACTACTACCCTTAA+TGG 0.258617 4.1:-19882193 None:intergenic
GAGTGTGAAAACAAAATATT+TGG 0.258800 4.1:+19882402 MS.gene028462:CDS
TTTGGAGCTTCAACATGATA+TGG 0.264232 4.1:+19882420 MS.gene028462:CDS
TAATGCATTATGCAACTAAA+TGG 0.300901 4.1:+19880625 MS.gene028462:CDS
AGCAACTCCATAGCAGAATT+TGG 0.307927 4.1:-19880142 None:intergenic
TTGACAGCAAGCAACATTTC+CGG 0.311281 4.1:+19880171 MS.gene028462:CDS
TGAAGCTTTCGTCGCTCTTC+CGG 0.314769 4.1:-19880436 None:intergenic
GAGAATGGCAAACATGTTAA+AGG 0.326315 4.1:+19880896 MS.gene028462:CDS
GCTGCAAGTTCAAAAGCTTC+AGG 0.338900 4.1:-19880120 None:intergenic
CTAGCCAACAGCTCTCTAAA+AGG 0.341374 4.1:+19882250 MS.gene028462:CDS
CGAAAGCTTCAAGCCCAAAA+TGG 0.344305 4.1:+19880448 MS.gene028462:CDS
AGTGATCTTCAAGTCCAAAT+TGG 0.350920 4.1:+19879534 MS.gene028462:CDS
TGTTGCTTGCTTCCTCTCCT+TGG 0.353914 4.1:-19880680 None:intergenic
CCTTCTCAGGCTTCTGAGAA+TGG 0.376751 4.1:+19880881 MS.gene028462:CDS
GTGCTAATCCAAAAGGAATA+AGG 0.382939 4.1:+19880403 MS.gene028462:CDS
AACAGGAAAACACTTCTAGA+AGG 0.384225 4.1:+19882307 MS.gene028462:CDS
CAGCAGCTGGAAGGGAATAT+GGG 0.385850 4.1:+19879286 MS.gene028462:CDS
GATACTCAGCAGCACATCTT+AGG 0.386146 4.1:-19880194 None:intergenic
TCAAAAGCTTCAGGCCCTCC+TGG 0.395690 4.1:-19880111 None:intergenic
TCTCGTGTAGCTTCCTTCTC+AGG 0.398409 4.1:+19880868 MS.gene028462:CDS
TTGAAGATGTTTCAATCCTT+AGG 0.401499 4.1:+19880529 MS.gene028462:CDS
TCAGAAAGGGATTGTCTACT+TGG 0.415289 4.1:-19881189 None:intergenic
CTTCTAGTTGTCTTTCTGTT+CGG 0.415753 4.1:-19882580 None:intergenic
AAATGTGGAAAATCATGATA+TGG 0.424301 4.1:+19882540 MS.gene028462:CDS
CTTTCATGCTCAGTTAGTGT+TGG 0.426046 4.1:-19882091 None:intergenic
TCCTGTTTGTTACCATTGGT+TGG 0.426066 4.1:-19882291 None:intergenic
GTGTCGTTCCCCGCCAGTCC+TGG 0.426869 4.1:-19880651 None:intergenic
CTATAGACTCGCTGCATCTT+CGG 0.430254 4.1:-19880368 None:intergenic
AACTAAATGGCTACCAGGAC+TGG 0.435309 4.1:+19880638 MS.gene028462:CDS
ATAGCTATCTTATTCAAGTC+GGG 0.438601 4.1:-19880078 None:intergenic
AAGCCTTGCCACTCTTGCCT+TGG 0.438871 4.1:-19881241 None:intergenic
AAATGAACGACTGCCATTAA+GGG 0.444360 4.1:+19882180 MS.gene028462:CDS
AGCTAGTGCCTCCTGATTGG+TGG 0.449333 4.1:+19880505 MS.gene028462:CDS
CCTGAAACTTTGTCGATGAA+AGG 0.449456 4.1:-19881302 None:intergenic
GGCACTAGCTGATTCCTGCT+CGG 0.457366 4.1:-19880492 None:intergenic
ATCAGCTAGTGCCTCCTGAT+TGG 0.466020 4.1:+19880502 MS.gene028462:CDS
CACATGCTTGTGATCAGAAA+GGG 0.471840 4.1:-19881202 None:intergenic
TGCATGCATGCAGGCGTCAA+TGG 0.474411 4.1:-19882152 None:intergenic
ACCAACCAATGGTAACAAAC+AGG 0.475079 4.1:+19882290 MS.gene028462:CDS
TGATTACTGATTTAGAGAAA+AGG 0.477093 4.1:+19881025 MS.gene028462:CDS
GATTACTGATTTAGAGAAAA+GGG 0.477757 4.1:+19881026 MS.gene028462:CDS
ACATCTTCAAACCACCAATC+AGG 0.482701 4.1:-19880516 None:intergenic
AGATTTGAACTGATTGGTGC+TGG 0.486553 4.1:+19880600 MS.gene028462:CDS
CGGGGTCACGAGAATCATAT+AGG 0.491631 4.1:-19880059 None:intergenic
AAATGGCTACCAGGACTGGC+GGG 0.494907 4.1:+19880642 MS.gene028462:CDS
ATTTCCTTCTTCTAGATCCT+CGG 0.497294 4.1:-19880226 None:intergenic
AAAGCTTGTGCTAATCCAAA+AGG 0.497825 4.1:+19880396 MS.gene028462:CDS
ATTTGGACTTGAAGATCACT+TGG 0.499439 4.1:-19879531 None:intergenic
GCACTAGCTGATTCCTGCTC+GGG 0.500897 4.1:-19880491 None:intergenic
GAAAAGATACCTTGTGCAAG+TGG 0.502085 4.1:-19879569 None:intergenic
CCACATGCTTGTGATCAGAA+AGG 0.508990 4.1:-19881203 None:intergenic
GAAGCGTCTCTGTCGTGTAA+TGG 0.510244 4.1:+19882114 MS.gene028462:CDS
CAACACTAACTGAGCATGAA+AGG 0.512884 4.1:+19882092 MS.gene028462:CDS
GGATCTTGTTCAGAGGTTGT+TGG 0.513765 4.1:+19881128 MS.gene028462:CDS
GGAATAAGGTGGGCGTATAC+CGG 0.514993 4.1:+19880417 MS.gene028462:CDS
GGCAAGAGTGGCAAGGCTTG+TGG 0.518056 4.1:+19881245 MS.gene028462:CDS
TCAGCTAGAGTTTCTGATGA+TGG 0.520535 4.1:+19881348 MS.gene028462:CDS
CATAGCTATCTTATTCAAGT+CGG 0.522649 4.1:-19880079 None:intergenic
ACTGATTTAGAGAAAAGGGT+TGG 0.526187 4.1:+19881030 MS.gene028462:CDS
GTGGTGACAACAACAGTAGC+TGG 0.528793 4.1:+19880724 MS.gene028462:CDS
AAGTGAAACAGCAGCTGGAA+GGG 0.532024 4.1:+19879278 MS.gene028462:CDS
GGTATGAGATTTGAACTGAT+TGG 0.534123 4.1:+19880594 MS.gene028462:CDS
ATAGCTATGGATGATCTCCC+AGG 0.534129 4.1:+19880093 MS.gene028462:CDS
CAAAAGCTTCAGGCCCTCCT+GGG 0.535576 4.1:-19880110 None:intergenic
CGACTTGAATAAGATAGCTA+TGG 0.536962 4.1:+19880080 MS.gene028462:CDS
AGCAAAAGCACACATCAGCT+TGG 0.538253 4.1:+19882476 MS.gene028462:CDS
CCTGAATGCCAAGGCAAGAG+TGG 0.539436 4.1:+19881233 MS.gene028462:CDS
TAACAAAGTGATCAATCCTA+AGG 0.539725 4.1:-19880545 None:intergenic
CGCAATCTTTCCAAGAAGGA+TGG 0.541041 4.1:+19882335 MS.gene028462:CDS
CGAACAGAAAGACAACTAGA+AGG 0.543947 4.1:+19882581 MS.gene028462:CDS
CACACATCAGCTTGGACTAG+TGG 0.544231 4.1:+19882484 MS.gene028462:CDS
TGAAAGACTCAAGCCCGAGC+AGG 0.544479 4.1:+19880478 MS.gene028462:CDS
CTCTGAAAAGCTGTCACCGT+GGG 0.544588 4.1:+19880326 MS.gene028462:CDS
CTTAACACCATGTTTAGTTG+AGG 0.545942 4.1:-19879322 None:intergenic
CTTGCAGCCAAATTCTGCTA+TGG 0.546691 4.1:+19880135 MS.gene028462:CDS
CTTGTTCAAGAACAAACTGA+AGG 0.548683 4.1:+19881159 MS.gene028462:CDS
TCAAACCAAGGATCAAAAGA+TGG 0.555075 4.1:+19880800 MS.gene028462:CDS
TCAGAAAGTGAAACAGCAGC+TGG 0.555263 4.1:+19879273 MS.gene028462:CDS
TATCCTTTGCTATCTAGAAG+TGG 0.555707 4.1:+19880021 MS.gene028462:CDS
CTCAGCAGCACATCTTAGGC+CGG 0.556722 4.1:-19880190 None:intergenic
ATGACCGAGGATCTAGAAGA+AGG 0.560265 4.1:+19880222 MS.gene028462:CDS
CCATTCTCAGAAGCCTGAGA+AGG 0.562143 4.1:-19880881 None:intergenic
AAACATGGTGTTAAGACTGA+AGG 0.569134 4.1:+19879330 MS.gene028462:CDS
AGCAATTGATTCATATCTCA+AGG 0.570287 4.1:+19881380 MS.gene028462:CDS
AAAGTGAAACAGCAGCTGGA+AGG 0.573228 4.1:+19879277 MS.gene028462:CDS
AGCAGCTGGAAGGGAATATG+GGG 0.577271 4.1:+19879287 MS.gene028462:CDS
CATCCTTCTTGGAAAGATTG+CGG 0.587019 4.1:-19882334 None:intergenic
ACACATCAGCTTGGACTAGT+GGG 0.588970 4.1:+19882485 MS.gene028462:CDS
GATGCAGCGAGTCTATAGCT+TGG 0.589137 4.1:+19880373 MS.gene028462:CDS
ACAGGAAAACACTTCTAGAA+GGG 0.589157 4.1:+19882308 MS.gene028462:CDS
AACATGGTGTTAAGACTGAA+GGG 0.589669 4.1:+19879331 MS.gene028462:CDS
TTCATCAAAGAGGCAACTCA+TGG 0.592342 4.1:+19879355 MS.gene028462:CDS
ACTTGCTAAAATGACAAATG+TGG 0.595036 4.1:+19882525 MS.gene028462:CDS
AACAACAGTAGCTGGAGAGG+TGG 0.595238 4.1:+19880732 MS.gene028462:CDS
TAAATGGCTACCAGGACTGG+CGG 0.596021 4.1:+19880641 MS.gene028462:CDS
TCAGAAACTCTAGCTGACTC+AGG 0.599469 4.1:-19881342 None:intergenic
TTTCCACTTCTAGATAGCAA+AGG 0.603021 4.1:-19880024 None:intergenic
AGCGAGTTTCCACTTGCACA+AGG 0.604757 4.1:+19879560 MS.gene028462:CDS
AATGGCTACCAGGACTGGCG+GGG 0.608558 4.1:+19880643 MS.gene028462:CDS
CCTTTCTGATCACAAGCATG+TGG 0.611442 4.1:+19881203 MS.gene028462:CDS
ACACCGCAATCTTTCCAAGA+AGG 0.615108 4.1:+19882331 MS.gene028462:CDS
GGGAACGACACTACAATCCA+AGG 0.616043 4.1:+19880663 MS.gene028462:CDS
ACTGAAGGGTTTCATCAAAG+AGG 0.617746 4.1:+19879345 MS.gene028462:CDS
ATGCCAAGGCAAGAGTGGCA+AGG 0.625949 4.1:+19881238 MS.gene028462:CDS
GACAACAACAGTAGCTGGAG+AGG 0.627220 4.1:+19880729 MS.gene028462:CDS
CTAATCCAAAAGGAATAAGG+TGG 0.630264 4.1:+19880406 MS.gene028462:CDS
CTTTCTGATCACAAGCATGT+GGG 0.642444 4.1:+19881204 MS.gene028462:CDS
TAGCTATCTTATTCAAGTCG+GGG 0.644788 4.1:-19880077 None:intergenic
ACAGAAAGACAACTAGAAGG+TGG 0.644997 4.1:+19882584 MS.gene028462:CDS
CTATGGATGATCTCCCAGGA+GGG 0.650157 4.1:+19880097 MS.gene028462:CDS
TGAATCTCAGTACCAACCAA+TGG 0.650255 4.1:+19882279 MS.gene028462:CDS
AACTTCAAGTCTTCAAACCA+AGG 0.650442 4.1:+19880788 MS.gene028462:CDS
TATGCAACTAAATGGCTACC+AGG 0.651310 4.1:+19880633 MS.gene028462:CDS
AAGTGAAACTATGTATGATG+TGG 0.652037 4.1:+19881107 MS.gene028462:CDS
GGGAAGTAACCTGAATGCCA+AGG 0.653383 4.1:+19881224 MS.gene028462:CDS
GTTCTCACCTCAACTAAACA+TGG 0.655729 4.1:+19879315 MS.gene028462:CDS
TGTGGACAGTTATCTTACAG+AGG 0.662209 4.1:+19881263 MS.gene028462:CDS
CGACACTACAATCCAAGGAG+AGG 0.663445 4.1:+19880668 MS.gene028462:CDS
GAGACACTGTCTTTCTGCGG+CGG 0.666070 4.1:-19880849 None:intergenic
CATCAGCTTGGACTAGTGGG+TGG 0.666857 4.1:+19882488 MS.gene028462:CDS
TAATCCAAAAGGAATAAGGT+GGG 0.670549 4.1:+19880407 MS.gene028462:CDS
TCTTCAAACCACCAATCAGG+AGG 0.670951 4.1:-19880513 None:intergenic
CACGAGACACTGTCTTTCTG+CGG 0.688596 4.1:-19880852 None:intergenic
GCTCTGAAAAGCTGTCACCG+TGG 0.705207 4.1:+19880325 MS.gene028462:CDS
ATGATGTGGATCTTGTTCAG+AGG 0.708047 4.1:+19881121 MS.gene028462:CDS
TGAGTATCTTGAAATGACCG+AGG 0.726304 4.1:+19880209 MS.gene028462:CDS
GCTATGGATGATCTCCCAGG+AGG 0.741106 4.1:+19880096 MS.gene028462:CDS
GCAGCTGGAAGGGAATATGG+GGG 0.752997 4.1:+19879288 MS.gene028462:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAACAAATATACTTATTT+AGG + chr4.1:19881618-19881637 MS.gene028462:intron 15.0%
!!! TTTTTATTTTCAATTACTCT+AGG + chr4.1:19881459-19881478 MS.gene028462:intron 15.0%
!! AATTAGTTAGACATTGTTTA+AGG - chr4.1:19880964-19880983 None:intergenic 20.0%
!! TATAAAGTACATAAATTCAG+TGG - chr4.1:19881660-19881679 None:intergenic 20.0%
!!! GTAAATTAATCACTTTTCAA+AGG + chr4.1:19881402-19881421 MS.gene028462:intron 20.0%
!!! GTTTCAAAAAAAAGTGATTT+TGG + chr4.1:19879729-19879748 MS.gene028462:intron 20.0%
!!! TAGCTATATTTTTCTTTGTT+TGG + chr4.1:19881917-19881936 MS.gene028462:intron 20.0%
! AAATGTGGAAAATCATGATA+TGG + chr4.1:19882540-19882559 MS.gene028462:CDS 25.0%
! AATATCAAACTATCTCTAAC+AGG + chr4.1:19880990-19881009 MS.gene028462:intron 25.0%
! ATTACTGCTATTAAGGTAAA+AGG + chr4.1:19880573-19880592 MS.gene028462:CDS 25.0%
! CTAAGATTCCAAGATAAAAT+TGG + chr4.1:19881682-19881701 MS.gene028462:intron 25.0%
! CTCATTGAAAATGAAATTTG+AGG - chr4.1:19881766-19881785 None:intergenic 25.0%
! GAGTGTGAAAACAAAATATT+TGG + chr4.1:19882402-19882421 MS.gene028462:CDS 25.0%
! TAATGCATTATGCAACTAAA+TGG + chr4.1:19880625-19880644 MS.gene028462:CDS 25.0%
! TAGAGTTATTACTGCTATTA+AGG + chr4.1:19880566-19880585 MS.gene028462:CDS 25.0%
! TCAATAGTATATCTTATCCT+GGG - chr4.1:19879652-19879671 None:intergenic 25.0%
! TCTTAAAAGCAAAGTTGTAT+TGG + chr4.1:19881547-19881566 MS.gene028462:intron 25.0%
!! GTAATGAGTTTCAATGATTT+TGG + chr4.1:19879698-19879717 MS.gene028462:intron 25.0%
!!! AAAAAGTGATTTTGGTATCA+CGG + chr4.1:19879737-19879756 MS.gene028462:intron 25.0%
!!! AATCACTTTTCAAAGGATAA+AGG + chr4.1:19881409-19881428 MS.gene028462:intron 25.0%
!!! AGTAATAGTAGTTTTAGTAG+TGG + chr4.1:19880705-19880724 MS.gene028462:CDS 25.0%
!!! ATCACTTTTCAAAGGATAAA+GGG + chr4.1:19881410-19881429 MS.gene028462:intron 25.0%
!!! CTCATTTTAGAAAAATGAGT+CGG + chr4.1:19881977-19881996 MS.gene028462:intron 25.0%
!!! GATTACTGATTTAGAGAAAA+GGG + chr4.1:19881026-19881045 MS.gene028462:CDS 25.0%
!!! TGATTACTGATTTAGAGAAA+AGG + chr4.1:19881025-19881044 MS.gene028462:CDS 25.0%
AAGTGAAACTATGTATGATG+TGG + chr4.1:19881107-19881126 MS.gene028462:CDS 30.0%
ACACAAGACATCAATGAAAT+TGG - chr4.1:19881956-19881975 None:intergenic 30.0%
AGATTCACATGATATGATCA+TGG + chr4.1:19882017-19882036 MS.gene028462:intron 30.0%
AGATTCCAAGATAAAATTGG+AGG + chr4.1:19881685-19881704 MS.gene028462:intron 30.0%
AGGATACTTTCACAACAAAA+AGG - chr4.1:19880007-19880026 None:intergenic 30.0%
AGGTAGTTACAAGTTAGTTA+TGG - chr4.1:19880944-19880963 None:intergenic 30.0%
ATAGCTATCTTATTCAAGTC+GGG - chr4.1:19880081-19880100 None:intergenic 30.0%
CAATGAAGGACAATTCATTT+GGG + chr4.1:19879811-19879830 MS.gene028462:intron 30.0%
CATAGCTATCTTATTCAAGT+CGG - chr4.1:19880082-19880101 None:intergenic 30.0%
CTATTGACATATAAACCAAG+TGG + chr4.1:19879665-19879684 MS.gene028462:intron 30.0%
GATCTGTGAAACAAATTAAC+AGG + chr4.1:19882063-19882082 MS.gene028462:intron 30.0%
GTAAAATTTGAGTCAGAACT+TGG + chr4.1:19881497-19881516 MS.gene028462:intron 30.0%
GTCAATAGTATATCTTATCC+TGG - chr4.1:19879653-19879672 None:intergenic 30.0%
GTCGTCTTTACTCTATAAAT+CGG + chr4.1:19879913-19879932 MS.gene028462:intron 30.0%
TAACAAAGTGATCAATCCTA+AGG - chr4.1:19880548-19880567 None:intergenic 30.0%
TAATCCAAAAGGAATAAGGT+GGG + chr4.1:19880407-19880426 MS.gene028462:CDS 30.0%
TCAATGAAGGACAATTCATT+TGG + chr4.1:19879810-19879829 MS.gene028462:intron 30.0%
TCTTTACTCTATAAATCGGA+AGG + chr4.1:19879917-19879936 MS.gene028462:intron 30.0%
TTCAGGCAACTTACATAAAA+TGG - chr4.1:19879469-19879488 None:intergenic 30.0%
TTGAAGATGTTTCAATCCTT+AGG + chr4.1:19880529-19880548 MS.gene028462:CDS 30.0%
! AATAACCATCTTTTGATCCT+TGG - chr4.1:19880808-19880827 None:intergenic 30.0%
! ACTTGCTAAAATGACAAATG+TGG + chr4.1:19882525-19882544 MS.gene028462:CDS 30.0%
! AGCAATTGATTCATATCTCA+AGG + chr4.1:19881380-19881399 MS.gene028462:CDS 30.0%
! CTTTCATTTCGTTCCATTTT+GGG - chr4.1:19880464-19880483 None:intergenic 30.0%
! TCTTTCATTTCGTTCCATTT+TGG - chr4.1:19880465-19880484 None:intergenic 30.0%
!! TCACTTTTCAAAGGATAAAG+GGG + chr4.1:19881411-19881430 MS.gene028462:intron 30.0%
!!! AAGCAAAGTTGTATTGGTTT+TGG + chr4.1:19881553-19881572 MS.gene028462:intron 30.0%
AAAATGAACGACTGCCATTA+AGG + chr4.1:19882179-19882198 MS.gene028462:CDS 35.0%
AAAGCTTGTGCTAATCCAAA+AGG + chr4.1:19880396-19880415 MS.gene028462:CDS 35.0%
AAATGAACGACTGCCATTAA+GGG + chr4.1:19882180-19882199 MS.gene028462:CDS 35.0%
AACAGGAAAACACTTCTAGA+AGG + chr4.1:19882307-19882326 MS.gene028462:CDS 35.0%
AACTTCAAGTCTTCAAACCA+AGG + chr4.1:19880788-19880807 MS.gene028462:CDS 35.0%
AGAGATATAATTGCGATGGA+GGG - chr4.1:19881725-19881744 None:intergenic 35.0%
AGTGATCTTCAAGTCCAAAT+TGG + chr4.1:19879534-19879553 MS.gene028462:CDS 35.0%
ATAGTATATCTTATCCTGGG+AGG - chr4.1:19879649-19879668 None:intergenic 35.0%
ATGCAGCTTCTAGTATATAG+TGG + chr4.1:19881831-19881850 MS.gene028462:intron 35.0%
ATGGCAAACATGTTAAAGGT+AGG + chr4.1:19880900-19880919 MS.gene028462:intron 35.0%
ATTTCCTTCTTCTAGATCCT+CGG - chr4.1:19880229-19880248 None:intergenic 35.0%
ATTTGGACTTGAAGATCACT+TGG - chr4.1:19879534-19879553 None:intergenic 35.0%
CGACTTGAATAAGATAGCTA+TGG + chr4.1:19880080-19880099 MS.gene028462:CDS 35.0%
CTAATCCAAAAGGAATAAGG+TGG + chr4.1:19880406-19880425 MS.gene028462:CDS 35.0%
CTTAACACCATGTTTAGTTG+AGG - chr4.1:19879325-19879344 None:intergenic 35.0%
CTTGTTCAAGAACAAACTGA+AGG + chr4.1:19881159-19881178 MS.gene028462:CDS 35.0%
GAGAATGGCAAACATGTTAA+AGG + chr4.1:19880896-19880915 MS.gene028462:CDS 35.0%
GCTTCTAGTATATAGTGGTA+TGG + chr4.1:19881836-19881855 MS.gene028462:intron 35.0%
GGACTTCATATGATTGTTTC+TGG + chr4.1:19880753-19880772 MS.gene028462:CDS 35.0%
GGTATGAGATTTGAACTGAT+TGG + chr4.1:19880594-19880613 MS.gene028462:CDS 35.0%
GTAAAAGAGAGGAAATGTTC+AGG - chr4.1:19879486-19879505 None:intergenic 35.0%
GTCAAACTTTACTTGTTTCC+TGG + chr4.1:19879871-19879890 MS.gene028462:intron 35.0%
GTGCTAATCCAAAAGGAATA+AGG + chr4.1:19880403-19880422 MS.gene028462:CDS 35.0%
TAGAGATATAATTGCGATGG+AGG - chr4.1:19881726-19881745 None:intergenic 35.0%
TAGCTATCTTATTCAAGTCG+GGG - chr4.1:19880080-19880099 None:intergenic 35.0%
TATACTAGAAGCTGCATTCA+CGG - chr4.1:19881828-19881847 None:intergenic 35.0%
TCAAACCAAGGATCAAAAGA+TGG + chr4.1:19880800-19880819 MS.gene028462:CDS 35.0%
TCCTTCATTGAGTGTATGTT+TGG - chr4.1:19879801-19879820 None:intergenic 35.0%
TCGTAGAGATATAATTGCGA+TGG - chr4.1:19881729-19881748 None:intergenic 35.0%
TTTCCACTTCTAGATAGCAA+AGG - chr4.1:19880027-19880046 None:intergenic 35.0%
! AAACATGGTGTTAAGACTGA+AGG + chr4.1:19879330-19879349 MS.gene028462:CDS 35.0%
! AACATGGTGTTAAGACTGAA+GGG + chr4.1:19879331-19879350 MS.gene028462:CDS 35.0%
! ACAGGAAAACACTTCTAGAA+GGG + chr4.1:19882308-19882327 MS.gene028462:CDS 35.0%
! ACTGATTTAGAGAAAAGGGT+TGG + chr4.1:19881030-19881049 MS.gene028462:CDS 35.0%
! ATTTCCTCTCTTTTACATGC+AGG + chr4.1:19879490-19879509 MS.gene028462:intron 35.0%
! ATTTGAAGATTTTCTGCCCA+CGG - chr4.1:19880345-19880364 None:intergenic 35.0%
! CTTCTAGTTGTCTTTCTGTT+CGG - chr4.1:19882583-19882602 None:intergenic 35.0%
! TATCCTTTGCTATCTAGAAG+TGG + chr4.1:19880021-19880040 MS.gene028462:CDS 35.0%
! TTTGGAGCTTCAACATGATA+TGG + chr4.1:19882420-19882439 MS.gene028462:CDS 35.0%
!! GTTAATTTACTTTTCCTCCC+AGG + chr4.1:19879632-19879651 MS.gene028462:intron 35.0%
!! GTTTCACTTTTCTCAAAGCA+AGG - chr4.1:19881096-19881115 None:intergenic 35.0%
!! GTTTTCCTGTTTGTTACCAT+TGG - chr4.1:19882298-19882317 None:intergenic 35.0%
!!! GCTATGTTGTTTTGTCTTCA+TGG + chr4.1:19880277-19880296 MS.gene028462:CDS 35.0%
AAGGTAGGCTGTGTTAGATA+TGG + chr4.1:19880915-19880934 MS.gene028462:intron 40.0%
ACAGAAAGACAACTAGAAGG+TGG + chr4.1:19882584-19882603 MS.gene028462:CDS 40.0%
ACATCTTCAAACCACCAATC+AGG - chr4.1:19880519-19880538 None:intergenic 40.0%
ACCAACCAATGGTAACAAAC+AGG + chr4.1:19882290-19882309 MS.gene028462:CDS 40.0%
ACTGAAGGGTTTCATCAAAG+AGG + chr4.1:19879345-19879364 MS.gene028462:CDS 40.0%
ACTTGCACTACTACCCTTAA+TGG - chr4.1:19882196-19882215 None:intergenic 40.0%
AGATTTGAACTGATTGGTGC+TGG + chr4.1:19880600-19880619 MS.gene028462:CDS 40.0%
AGCAACTCCATAGCAGAATT+TGG - chr4.1:19880145-19880164 None:intergenic 40.0%
ATCCTGACTGGAACAATCTT+TGG + chr4.1:19879849-19879868 MS.gene028462:intron 40.0%
ATGATGTGGATCTTGTTCAG+AGG + chr4.1:19881121-19881140 MS.gene028462:CDS 40.0%
ATTACTTGAGCTCAACCACT+TGG - chr4.1:19879683-19879702 None:intergenic 40.0%
ATTGCGATGGAGGGATATAA+TGG - chr4.1:19881716-19881735 None:intergenic 40.0%
CAACACTAACTGAGCATGAA+AGG + chr4.1:19882092-19882111 MS.gene028462:CDS 40.0%
CACATGCTTGTGATCAGAAA+GGG - chr4.1:19881205-19881224 None:intergenic 40.0%
CATACCTGCATGTAAAAGAG+AGG - chr4.1:19879497-19879516 None:intergenic 40.0%
CATCCTTCTTGGAAAGATTG+CGG - chr4.1:19882337-19882356 None:intergenic 40.0%
CCTGAAACTTTGTCGATGAA+AGG - chr4.1:19881305-19881324 None:intergenic 40.0%
CCTTTCATCGACAAAGTTTC+AGG + chr4.1:19881302-19881321 MS.gene028462:CDS 40.0%
CGAACAGAAAGACAACTAGA+AGG + chr4.1:19882581-19882600 MS.gene028462:CDS 40.0%
CTGTTCGGTTCTTCAGAAAT+TGG - chr4.1:19882568-19882587 None:intergenic 40.0%
CTTTCATGCTCAGTTAGTGT+TGG - chr4.1:19882094-19882113 None:intergenic 40.0%
CTTTCTGATCACAAGCATGT+GGG + chr4.1:19881204-19881223 MS.gene028462:CDS 40.0%
GAAAAGATACCTTGTGCAAG+TGG - chr4.1:19879572-19879591 None:intergenic 40.0%
GCCAAACATACACTCAATGA+AGG + chr4.1:19879797-19879816 MS.gene028462:intron 40.0%
GTAAAGACGACCAAGTAATC+CGG - chr4.1:19879904-19879923 None:intergenic 40.0%
GTTCTCACCTCAACTAAACA+TGG + chr4.1:19879315-19879334 MS.gene028462:CDS 40.0%
TATGCAACTAAATGGCTACC+AGG + chr4.1:19880633-19880652 MS.gene028462:CDS 40.0%
TCAGAAAGGGATTGTCTACT+TGG - chr4.1:19881192-19881211 None:intergenic 40.0%
TCAGCTAGAGTTTCTGATGA+TGG + chr4.1:19881348-19881367 MS.gene028462:CDS 40.0%
TGAATCTCAGTACCAACCAA+TGG + chr4.1:19882279-19882298 MS.gene028462:CDS 40.0%
TGAGTATCTTGAAATGACCG+AGG + chr4.1:19880209-19880228 MS.gene028462:CDS 40.0%
TGAGTGTATGTTTGGCATCA+CGG - chr4.1:19879793-19879812 None:intergenic 40.0%
TGTGGACAGTTATCTTACAG+AGG + chr4.1:19881263-19881282 MS.gene028462:CDS 40.0%
TTCATCAAAGAGGCAACTCA+TGG + chr4.1:19879355-19879374 MS.gene028462:CDS 40.0%
TTGACAGCAAGCAACATTTC+CGG + chr4.1:19880171-19880190 MS.gene028462:CDS 40.0%
! CGCTTCCTCCAATTTTATCT+TGG - chr4.1:19881693-19881712 None:intergenic 40.0%
! TCCTGTTTGTTACCATTGGT+TGG - chr4.1:19882294-19882313 None:intergenic 40.0%
AAAGTGAAACAGCAGCTGGA+AGG + chr4.1:19879277-19879296 MS.gene028462:CDS 45.0%
AACTAAATGGCTACCAGGAC+TGG + chr4.1:19880638-19880657 MS.gene028462:CDS 45.0%
AAGTGAAACAGCAGCTGGAA+GGG + chr4.1:19879278-19879297 MS.gene028462:CDS 45.0%
ACACCGCAATCTTTCCAAGA+AGG + chr4.1:19882331-19882350 MS.gene028462:CDS 45.0%
ATAGCTATGGATGATCTCCC+AGG + chr4.1:19880093-19880112 MS.gene028462:CDS 45.0%
ATGACCGAGGATCTAGAAGA+AGG + chr4.1:19880222-19880241 MS.gene028462:CDS 45.0%
CCACATGCTTGTGATCAGAA+AGG - chr4.1:19881206-19881225 None:intergenic 45.0%
CCTTTCTGATCACAAGCATG+TGG + chr4.1:19881203-19881222 MS.gene028462:CDS 45.0%
CGAAAGCTTCAAGCCCAAAA+TGG + chr4.1:19880448-19880467 MS.gene028462:CDS 45.0%
CGCAATCTTTCCAAGAAGGA+TGG + chr4.1:19882335-19882354 MS.gene028462:CDS 45.0%
CTAGCCAACAGCTCTCTAAA+AGG + chr4.1:19882250-19882269 MS.gene028462:CDS 45.0%
CTATAGACTCGCTGCATCTT+CGG - chr4.1:19880371-19880390 None:intergenic 45.0%
CTTGCAGCCAAATTCTGCTA+TGG + chr4.1:19880135-19880154 MS.gene028462:CDS 45.0%
GACCAAAGATTGTTCCAGTC+AGG - chr4.1:19879854-19879873 None:intergenic 45.0%
GATACTCAGCAGCACATCTT+AGG - chr4.1:19880197-19880216 None:intergenic 45.0%
GCTGCAAGTTCAAAAGCTTC+AGG - chr4.1:19880123-19880142 None:intergenic 45.0%
GGAAACTCGCTTCTCCAATT+TGG - chr4.1:19879551-19879570 None:intergenic 45.0%
GGATCTTGTTCAGAGGTTGT+TGG + chr4.1:19881128-19881147 MS.gene028462:CDS 45.0%
TAGCTGTTGTCCATCCTTCT+TGG - chr4.1:19882348-19882367 None:intergenic 45.0%
TCAGAAAGTGAAACAGCAGC+TGG + chr4.1:19879273-19879292 MS.gene028462:CDS 45.0%
TCTTCAAACCACCAATCAGG+AGG - chr4.1:19880516-19880535 None:intergenic 45.0%
TGTATGTTTGGCATCACGGT+GGG - chr4.1:19879789-19879808 None:intergenic 45.0%
TTCCAGTCAGGATTCGATCT+CGG - chr4.1:19879842-19879861 None:intergenic 45.0%
! AGCAAAAGCACACATCAGCT+TGG + chr4.1:19882476-19882495 MS.gene028462:CDS 45.0%
! ATTTTGAGCTGCATGCATGC+AGG - chr4.1:19882164-19882183 None:intergenic 45.0%
! TACGCCCACCTTATTCCTTT+TGG - chr4.1:19880414-19880433 None:intergenic 45.0%
! TCAGAAACTCTAGCTGACTC+AGG - chr4.1:19881345-19881364 None:intergenic 45.0%
!! ACACATCAGCTTGGACTAGT+GGG + chr4.1:19882485-19882504 MS.gene028462:CDS 45.0%
!! CGGTGGGTTTGTTAGAATCA+CGG - chr4.1:19879773-19879792 None:intergenic 45.0%
!! TGGGTTTGTTAGAATCACGG+TGG - chr4.1:19879770-19879789 None:intergenic 45.0%
AACAACAGTAGCTGGAGAGG+TGG + chr4.1:19880732-19880751 MS.gene028462:CDS 50.0%
ACAGCAGCTGGAAGGGAATA+TGG + chr4.1:19879285-19879304 MS.gene028462:CDS 50.0%
AGCAGCTGGAAGGGAATATG+GGG + chr4.1:19879287-19879306 MS.gene028462:CDS 50.0%
AGCGAGTTTCCACTTGCACA+AGG + chr4.1:19879560-19879579 MS.gene028462:CDS 50.0%
ATCAGCTAGTGCCTCCTGAT+TGG + chr4.1:19880502-19880521 MS.gene028462:CDS 50.0%
CACGAGACACTGTCTTTCTG+CGG - chr4.1:19880855-19880874 None:intergenic 50.0%
CAGCAGCTGGAAGGGAATAT+GGG + chr4.1:19879286-19879305 MS.gene028462:CDS 50.0%
CCATTCTCAGAAGCCTGAGA+AGG - chr4.1:19880884-19880903 None:intergenic 50.0%
CGACACTACAATCCAAGGAG+AGG + chr4.1:19880668-19880687 MS.gene028462:CDS 50.0%
CGACCAAGTAATCCGGAGTT+CGG - chr4.1:19879897-19879916 None:intergenic 50.0%
CGGGGTCACGAGAATCATAT+AGG - chr4.1:19880062-19880081 None:intergenic 50.0%
CTATGGATGATCTCCCAGGA+GGG + chr4.1:19880097-19880116 MS.gene028462:CDS 50.0%
GACAACAACAGTAGCTGGAG+AGG + chr4.1:19880729-19880748 MS.gene028462:CDS 50.0%
GATGCAGCGAGTCTATAGCT+TGG + chr4.1:19880373-19880392 MS.gene028462:CDS 50.0%
GCTGCATTCACGGTAGAATC+AGG - chr4.1:19881818-19881837 None:intergenic 50.0%
GGAATAAGGTGGGCGTATAC+CGG + chr4.1:19880417-19880436 MS.gene028462:CDS 50.0%
GGGAACGACACTACAATCCA+AGG + chr4.1:19880663-19880682 MS.gene028462:CDS 50.0%
GGGAAGTAACCTGAATGCCA+AGG + chr4.1:19881224-19881243 MS.gene028462:CDS 50.0%
GTGGTGACAACAACAGTAGC+TGG + chr4.1:19880724-19880743 MS.gene028462:CDS 50.0%
GTGTATGTTTGGCATCACGG+TGG - chr4.1:19879790-19879809 None:intergenic 50.0%
TAAATGGCTACCAGGACTGG+CGG + chr4.1:19880641-19880660 MS.gene028462:CDS 50.0%
TCCAGTCAGGATTCGATCTC+GGG - chr4.1:19879841-19879860 None:intergenic 50.0%
TCTCGTGTAGCTTCCTTCTC+AGG + chr4.1:19880868-19880887 MS.gene028462:CDS 50.0%
TGAAGCTTTCGTCGCTCTTC+CGG - chr4.1:19880439-19880458 None:intergenic 50.0%
! CACACATCAGCTTGGACTAG+TGG + chr4.1:19882484-19882503 MS.gene028462:CDS 50.0%
! CCTTCTCAGGCTTCTGAGAA+TGG + chr4.1:19880881-19880900 MS.gene028462:CDS 50.0%
! CTCTGAAAAGCTGTCACCGT+GGG + chr4.1:19880326-19880345 MS.gene028462:CDS 50.0%
! GAAGCGTCTCTGTCGTGTAA+TGG + chr4.1:19882114-19882133 MS.gene028462:CDS 50.0%
! TGTTGCTTGCTTCCTCTCCT+TGG - chr4.1:19880683-19880702 None:intergenic 50.0%
!! GCTGCCTTTTAGAGAGCTGT+TGG - chr4.1:19882257-19882276 None:intergenic 50.0%
AAGCCTTGCCACTCTTGCCT+TGG - chr4.1:19881244-19881263 None:intergenic 55.0%
AAGTAATCCGGAGTTCGGCC+AGG - chr4.1:19879892-19879911 None:intergenic 55.0%
ACCCGAGATCGAATCCTGAC+TGG + chr4.1:19879837-19879856 MS.gene028462:intron 55.0%
AGCTAGTGCCTCCTGATTGG+TGG + chr4.1:19880505-19880524 MS.gene028462:CDS 55.0%
ATGCCAAGGCAAGAGTGGCA+AGG + chr4.1:19881238-19881257 MS.gene028462:CDS 55.0%
CAAAAGCTTCAGGCCCTCCT+GGG - chr4.1:19880113-19880132 None:intergenic 55.0%
CCACTCTTGCCTTGGCATTC+AGG - chr4.1:19881236-19881255 None:intergenic 55.0%
CCTGAATGCCAAGGCAAGAG+TGG + chr4.1:19881233-19881252 MS.gene028462:CDS 55.0%
CTCAGCAGCACATCTTAGGC+CGG - chr4.1:19880193-19880212 None:intergenic 55.0%
CTTGTTTCCTGGCCGAACTC+CGG + chr4.1:19879882-19879901 MS.gene028462:intron 55.0%
GAGACACTGTCTTTCTGCGG+CGG - chr4.1:19880852-19880871 None:intergenic 55.0%
GCAGCTGGAAGGGAATATGG+GGG + chr4.1:19879288-19879307 MS.gene028462:CDS 55.0%
GCTATGGATGATCTCCCAGG+AGG + chr4.1:19880096-19880115 MS.gene028462:CDS 55.0%
TCAAAAGCTTCAGGCCCTCC+TGG - chr4.1:19880114-19880133 None:intergenic 55.0%
TGAAAGACTCAAGCCCGAGC+AGG + chr4.1:19880478-19880497 MS.gene028462:CDS 55.0%
TGCATGCATGCAGGCGTCAA+TGG - chr4.1:19882155-19882174 None:intergenic 55.0%
TGGCCGAACTCCGGATTACT+TGG + chr4.1:19879891-19879910 MS.gene028462:intron 55.0%
! AAATGGCTACCAGGACTGGC+GGG + chr4.1:19880642-19880661 MS.gene028462:CDS 55.0%
! GCACTAGCTGATTCCTGCTC+GGG - chr4.1:19880494-19880513 None:intergenic 55.0%
! GGCACTAGCTGATTCCTGCT+CGG - chr4.1:19880495-19880514 None:intergenic 55.0%
!! CATCAGCTTGGACTAGTGGG+TGG + chr4.1:19882488-19882507 MS.gene028462:CDS 55.0%
!! GCTCTGAAAAGCTGTCACCG+TGG + chr4.1:19880325-19880344 MS.gene028462:CDS 55.0%
GCTGGAAGGGAATATGGGGG+TGG + chr4.1:19879291-19879310 MS.gene028462:CDS 60.0%
GGCAAGAGTGGCAAGGCTTG+TGG + chr4.1:19881245-19881264 MS.gene028462:CDS 60.0%
! AATGGCTACCAGGACTGGCG+GGG + chr4.1:19880643-19880662 MS.gene028462:CDS 60.0%
! GTGTCGTTCCCCGCCAGTCC+TGG - chr4.1:19880654-19880673 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 19879264 19882624 19879264 ID=MS.gene028462
chr4.1 mRNA 19879264 19882624 19879264 ID=MS.gene028462.t1;Parent=MS.gene028462
chr4.1 exon 19879264 19879376 19879264 ID=MS.gene028462.t1.exon1;Parent=MS.gene028462.t1
chr4.1 CDS 19879264 19879376 19879264 ID=cds.MS.gene028462.t1;Parent=MS.gene028462.t1
chr4.1 exon 19879512 19879581 19879512 ID=MS.gene028462.t1.exon2;Parent=MS.gene028462.t1
chr4.1 CDS 19879512 19879581 19879512 ID=cds.MS.gene028462.t1;Parent=MS.gene028462.t1
chr4.1 exon 19880021 19880917 19880021 ID=MS.gene028462.t1.exon3;Parent=MS.gene028462.t1
chr4.1 CDS 19880021 19880917 19880021 ID=cds.MS.gene028462.t1;Parent=MS.gene028462.t1
chr4.1 exon 19881012 19881401 19881012 ID=MS.gene028462.t1.exon4;Parent=MS.gene028462.t1
chr4.1 CDS 19881012 19881401 19881012 ID=cds.MS.gene028462.t1;Parent=MS.gene028462.t1
chr4.1 exon 19882085 19882624 19882085 ID=MS.gene028462.t1.exon5;Parent=MS.gene028462.t1
chr4.1 CDS 19882085 19882624 19882085 ID=cds.MS.gene028462.t1;Parent=MS.gene028462.t1
Gene Sequence

>MS.gene028462

ATGTGGGAATCAGAAAGTGAAACAGCAGCTGGAAGGGAATATGGGGGTGGAGTTCTCACCTCAACTAAACATGGTGTTAAGACTGAAGGGTTTCATCAAAGAGGCAACTCATGGTATGTTAATGTTTAATTCATGCAAAAAAAATCATTATTGAAACTTCTAAAATGATCATAATATTTTCTTTTTTGAACTTTTGATTTATCCATTTTATGTAAGTTGCCTGAACATTTCCTCTCTTTTACATGCAGGTATGTTTCAGCTGATATTCCAAGTGATCTTCAAGTCCAAATTGGAGAAGCGAGTTTCCACTTGCACAAGGTATCTTTTCTTTTGATATTTCAAGTACTTCTTTTTTTATTTCTTAGAAAGTTAATTTACTTTTCCTCCCAGGATAAGATATACTATTGACATATAAACCAAGTGGTTGAGCTCAAGTAATGAGTTTCAATGATTTTGGTATCACGTGTTTCAAAAAAAAGTGATTTTGGTATCACGGTGAATTTCCACCGTGATTCTAACAAACCCACCGTGATGCCAAACATACACTCAATGAAGGACAATTCATTTGGGAGAACCCGAGATCGAATCCTGACTGGAACAATCTTTGGTCAAACTTTACTTGTTTCCTGGCCGAACTCCGGATTACTTGGTCGTCTTTACTCTATAAATCGGAAGGTTAAGAAAAAAAGTTACTACTGATCATCTGTACTGTATTGTGTTGAATTTGATGAACTTTTCTTCCTTTTTGTTGTGAAAGTATCCTTTGCTATCTAGAAGTGGAAAGCTGAACAGAATCCTATATGATTCTCGTGACCCCGACTTGAATAAGATAGCTATGGATGATCTCCCAGGAGGGCCTGAAGCTTTTGAACTTGCAGCCAAATTCTGCTATGGAGTTGCTATTGATTTGACAGCAAGCAACATTTCCGGCCTAAGATGTGCTGCTGAGTATCTTGAAATGACCGAGGATCTAGAAGAAGGAAATCTTATATTCAAGACTGAAGCTTTTCTTAGCTATGTTGTTTTGTCTTCATGGAGAGACTCTATAGTAGTGTTGAAAAGCTCTGAAAAGCTGTCACCGTGGGCAGAAAATCTTCAAATTGTCCGAAGATGCAGCGAGTCTATAGCTTGGAAAGCTTGTGCTAATCCAAAAGGAATAAGGTGGGCGTATACCGGAAGAGCGACGAAAGCTTCAAGCCCAAAATGGAACGAAATGAAAGACTCAAGCCCGAGCAGGAATCAGCTAGTGCCTCCTGATTGGTGGTTTGAAGATGTTTCAATCCTTAGGATTGATCACTTTGTTAGAGTTATTACTGCTATTAAGGTAAAAGGTATGAGATTTGAACTGATTGGTGCTGGAATAATGCATTATGCAACTAAATGGCTACCAGGACTGGCGGGGAACGACACTACAATCCAAGGAGAGGAAGCAAGCAACAACAGTAATAGTAGTTTTAGTAGTGGTGACAACAACAGTAGCTGGAGAGGTGGACTTCATATGATTGTTTCTGGAACTAGAGATGAAACTTCAAGTCTTCAAACCAAGGATCAAAAGATGGTTATTGAGAGTCTCATCAGCATAATTCCGCCGCAGAAAGACAGTGTCTCGTGTAGCTTCCTTCTCAGGCTTCTGAGAATGGCAAACATGTTAAAGGTAGGCTGTGTTAGATATGGCAACCATAACTAACTTGTAACTACCTTAAACAATGTCTAACTAATTCTAACAAATATCAAACTATCTCTAACAGGTAGCATATGCATTGATTACTGATTTAGAGAAAAGGGTTGGAATGCAGTTTGAGCAAGCTACACTTTCTGATCTTCTAATTCCTTGCTTTGAGAAAAGTGAAACTATGTATGATGTGGATCTTGTTCAGAGGTTGTTGGAGCATTTTCTTGTTCAAGAACAAACTGAAGGTTATAGTCCAAGTAGACAATCCCTTTCTGATCACAAGCATGTGGGAAGTAACCTGAATGCCAAGGCAAGAGTGGCAAGGCTTGTGGACAGTTATCTTACAGAGGTATCTAGAGATAGAAACCTTTCATCGACAAAGTTTCAGGTTCTTGCAGAAGCTTTACCTGAGTCAGCTAGAGTTTCTGATGATGGACTATACAGAGCAATTGATTCATATCTCAAGGTAAATTAATCACTTTTCAAAGGATAAAGGGGAACAATCTTTCAAGTTTCTTATCATTTTTTATTTTCAATTACTCTAGGATACATGAACTTTAAGTAAAATTTGAGTCAGAACTTGGAGTTTCATAATATGCTCATTATACACATCTTAAAAGCAAAGTTGTATTGGTTTTGGCATCTTTGTTTAAGAAATGTGCATGTATTCACATTCTGAAATATGAACAAATATACTTATTTAGGTTCTATAGTTAATTGACCACTGAATTTATGTACTTTATATGCTAAGATTCCAAGATAAAATTGGAGGAAGCGCCATTATATCCCTCCATCGCAATTATATCTCTACGACAAGATTACAAAAGCCTCAAATTTCATTTTCAATGAGATTACATTTGACGTTAAGTATAAACTTTGCCTGATTCTACCGTGAATGCAGCTTCTAGTATATAGTGGTATGGTATTTACAAAAGATATGAATTTAGTAAACACATAATCAATCATTTCTCTAGAAAAGATTAGCTATATTTTTCTTTGTTTGGAACAATAAATGCACCAATTTCATTGATGTCTTGTGTACTCATTTTAGAAAAATGAGTCGGAACAATTAGTATAGTTTAGATTCACATGATATGATCATGGATTTATATTTCTTTACTCATGACGATCTGTGAAACAAATTAACAGGCACATCCAACACTAACTGAGCATGAAAGGAAGCGTCTCTGTCGTGTAATGGATTGTCAAAAACTCTCCATTGACGCCTGCATGCATGCAGCTCAAAATGAACGACTGCCATTAAGGGTAGTAGTGCAAGTTCTATTCTCTGAACAAGTAAAGATAAGCAACGCACTAGCCAACAGCTCTCTAAAAGGCAGCGTTGAATCTCAGTACCAACCAATGGTAACAAACAGGAAAACACTTCTAGAAGGGACACCGCAATCTTTCCAAGAAGGATGGACAACAGCTAAGAAAGACATTAACACATTAAAGTTTGAACTTGAGAGTGTGAAAACAAAATATTTGGAGCTTCAACATGATATGGAAAATCTGCAGAAAAATTTCGATAAGCTGTTGAAGCAAAAGCACACATCAGCTTGGACTAGTGGGTGGAAGAAACTGAGCAAACTTGCTAAAATGACAAATGTGGAAAATCATGATATGGTTCCAATTTCTGAAGAACCGAACAGAAAGACAACTAGAAGGTGGAGAAACTCAATATCTTGA
Protein sequence

>MS.gene028462.t1

MWESESETAAGREYGGGVLTSTKHGVKTEGFHQRGNSWYVSADIPSDLQVQIGEASFHLHKYPLLSRSGKLNRILYDSRDPDLNKIAMDDLPGGPEAFELAAKFCYGVAIDLTASNISGLRCAAEYLEMTEDLEEGNLIFKTEAFLSYVVLSSWRDSIVVLKSSEKLSPWAENLQIVRRCSESIAWKACANPKGIRWAYTGRATKASSPKWNEMKDSSPSRNQLVPPDWWFEDVSILRIDHFVRVITAIKVKGMRFELIGAGIMHYATKWLPGLAGNDTTIQGEEASNNSNSSFSSGDNNSSWRGGLHMIVSGTRDETSSLQTKDQKMVIESLISIIPPQKDSVSCSFLLRLLRMANMLKVAYALITDLEKRVGMQFEQATLSDLLIPCFEKSETMYDVDLVQRLLEHFLVQEQTEGYSPSRQSLSDHKHVGSNLNAKARVARLVDSYLTEVSRDRNLSSTKFQVLAEALPESARVSDDGLYRAIDSYLKAHPTLTEHERKRLCRVMDCQKLSIDACMHAAQNERLPLRVVVQVLFSEQVKISNALANSSLKGSVESQYQPMVTNRKTLLEGTPQSFQEGWTTAKKDINTLKFELESVKTKYLELQHDMENLQKNFDKLLKQKHTSAWTSGWKKLSKLAKMTNVENHDMVPISEEPNRKTTRRWRNSIS