AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35341

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35341.t1 MTR_2g084595 96.057 634 13 2 1 622 1 634 0.0 1246
MS.gene35341.t1 MTR_2g084595 95.964 446 10 1 1 438 1 446 0.0 879
MS.gene35341.t1 MTR_1g094850 39.899 594 322 12 8 591 19 587 4.72e-147 439
MS.gene35341.t1 MTR_8g078900 39.308 636 320 15 10 591 15 638 5.08e-147 441
MS.gene35341.t1 MTR_2g048390 47.926 434 218 5 10 437 21 452 1.05e-143 425
MS.gene35341.t1 MTR_8g078900 38.871 620 313 15 26 591 1 608 1.25e-139 421
MS.gene35341.t1 MTR_8g078900 40.070 569 293 13 59 591 42 598 1.30e-136 412
MS.gene35341.t1 MTR_2g018750 38.448 567 309 10 13 565 24 564 6.54e-135 407
MS.gene35341.t1 MTR_4g093850 36.860 605 326 11 6 564 27 621 5.59e-133 405
MS.gene35341.t1 MTR_1g064360 36.445 557 302 9 13 558 24 539 8.68e-121 370
MS.gene35341.t1 MTR_2g009340 35.738 610 335 12 8 571 37 635 1.15e-120 373
MS.gene35341.t1 MTR_4g022330 36.282 554 320 8 15 559 34 563 1.61e-120 371
MS.gene35341.t1 MTR_5g016060 36.042 566 325 14 15 572 40 576 1.24e-112 351
MS.gene35341.t1 MTR_7g073980 34.091 616 355 15 10 619 30 600 1.63e-109 343
MS.gene35341.t1 MTR_5g032780 38.000 500 278 8 13 486 24 517 3.06e-105 332
MS.gene35341.t1 MTR_7g108290 34.412 587 335 12 13 579 34 590 3.24e-105 333
MS.gene35341.t1 MTR_1g088885 34.179 591 330 12 13 580 34 588 5.71e-102 323
MS.gene35341.t1 MTR_4g093850 36.983 484 253 10 6 447 27 500 6.82e-101 317
MS.gene35341.t1 MTR_8g078900 38.562 459 241 10 10 437 15 463 8.17e-101 315
MS.gene35341.t1 MTR_8g090220 30.201 596 334 10 8 570 45 591 4.47e-100 318
MS.gene35341.t1 MTR_5g015790 38.509 483 260 9 15 487 27 482 9.73e-100 317
MS.gene35341.t1 MTR_4g095430 33.271 538 334 8 15 550 13 527 1.64e-99 315
MS.gene35341.t1 MTR_8g090220 32.937 504 280 7 8 489 45 512 2.54e-98 310
MS.gene35341.t1 MTR_1g106430 35.547 512 284 10 13 512 26 503 5.95e-95 305
MS.gene35341.t1 MTR_1g014070 34.151 489 298 6 5 487 17 487 9.47e-95 304
MS.gene35341.t1 MTR_3g062540 32.342 538 312 9 15 551 24 510 1.49e-90 291
MS.gene35341.t1 MTR_8g078900 39.541 392 214 8 59 437 42 423 1.69e-90 286
MS.gene35341.t1 MTR_8g069330 34.959 492 259 9 18 486 29 482 4.19e-89 288
MS.gene35341.t1 MTR_5g016060 35.246 488 283 12 92 572 1 462 7.85e-89 285
MS.gene35341.t1 MTR_4g134210 34.874 476 282 9 13 486 24 473 8.03e-88 285
MS.gene35341.t1 MTR_5g075640 33.733 584 316 13 15 585 25 550 3.92e-87 283
MS.gene35341.t1 MTR_3g062540 32.046 518 300 9 35 551 2 468 4.27e-84 273
MS.gene35341.t1 MTR_5g075640 33.520 537 290 12 61 585 26 507 5.20e-80 263
MS.gene35341.t1 MTR_1g082690 28.727 550 329 7 17 564 60 548 2.23e-79 261
MS.gene35341.t1 MTR_2g027410 32.000 475 287 9 13 486 24 463 1.02e-73 247
MS.gene35341.t1 MTR_2g027410 32.075 424 254 8 64 486 1 391 1.38e-61 213
MS.gene35341.t1 MTR_4g045767 28.720 578 344 18 12 568 2 532 1.08e-56 201
MS.gene35341.t1 MTR_4g011530 36.177 293 162 4 201 489 43 314 8.50e-53 187
MS.gene35341.t1 MTR_4g074190 27.181 493 313 9 18 492 6 470 1.63e-50 184
MS.gene35341.t1 MTR_1g035720 25.664 565 333 18 28 564 17 522 4.28e-50 182
MS.gene35341.t1 MTR_2g102730 35.172 290 165 4 204 489 24 294 2.20e-47 172
MS.gene35341.t1 MTR_4g074190 27.212 452 285 7 57 492 1 424 5.88e-46 170
MS.gene35341.t1 MTR_4g074190 26.818 440 276 9 18 439 6 417 2.28e-41 156
MS.gene35341.t1 MTR_2g010890 25.806 403 243 10 83 474 1 358 2.27e-31 127
MS.gene35341.t1 MTR_2g048375 40.341 176 88 7 454 622 1 166 5.47e-30 116
MS.gene35341.t1 MTR_1g105870 28.696 230 134 3 19 248 35 234 3.82e-21 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35341.t1 AT3G44820 63.538 650 207 11 1 622 1 648 0.0 813
MS.gene35341.t1 AT1G30440 45.862 580 292 8 10 571 21 596 2.75e-178 521
MS.gene35341.t1 AT5G03250 41.681 583 297 12 13 582 24 576 1.05e-146 437
MS.gene35341.t1 AT5G66560 37.895 665 316 15 10 595 14 660 5.39e-136 412
MS.gene35341.t1 AT5G13600 40.070 574 290 15 13 570 24 559 7.94e-136 409
MS.gene35341.t1 AT5G66560 37.596 649 308 15 26 595 1 631 3.44e-129 394
MS.gene35341.t1 AT3G50840 37.896 599 314 14 10 597 11 562 7.69e-129 391
MS.gene35341.t1 AT5G66560 40.070 574 289 13 59 595 66 621 7.74e-128 390
MS.gene35341.t1 AT5G64330 33.954 698 352 15 8 607 45 731 4.55e-124 384
MS.gene35341.t1 AT5G64330 36.806 576 268 11 8 495 45 612 1.03e-118 368
MS.gene35341.t1 AT5G48800 35.802 567 312 9 15 569 41 567 2.43e-116 360
MS.gene35341.t1 AT3G50840 37.774 548 288 12 59 597 23 526 2.60e-115 355
MS.gene35341.t1 AT3G50840 37.774 548 288 12 59 597 23 526 2.60e-115 355
MS.gene35341.t1 AT5G03250 39.879 494 254 12 102 582 1 464 2.59e-111 342
MS.gene35341.t1 AT5G64330 35.971 556 260 11 28 495 6 553 2.03e-109 342
MS.gene35341.t1 AT3G08570 34.974 569 312 10 10 559 31 560 8.27e-109 340
MS.gene35341.t1 AT3G08660 35.612 556 298 9 10 559 31 532 1.77e-108 338
MS.gene35341.t1 AT1G03010 33.333 633 365 13 13 620 7 607 8.41e-102 322
MS.gene35341.t1 AT1G03010 33.333 633 365 13 13 620 33 633 2.17e-101 322
MS.gene35341.t1 AT1G03010 33.333 633 365 13 13 620 34 634 2.59e-101 322
MS.gene35341.t1 AT5G67385 34.206 535 320 9 10 541 20 525 5.79e-101 320
MS.gene35341.t1 AT2G14820 35.155 549 296 13 10 532 22 536 1.27e-99 317
MS.gene35341.t1 AT2G14820 35.155 549 296 13 10 532 22 536 1.27e-99 317
MS.gene35341.t1 AT3G08660 34.667 525 282 8 41 559 3 472 1.87e-97 308
MS.gene35341.t1 AT2G30520 34.637 537 308 8 15 550 30 524 1.96e-97 310
MS.gene35341.t1 AT5G67440 36.570 484 269 9 15 491 27 479 6.14e-94 301
MS.gene35341.t1 AT5G67440 36.570 484 269 9 15 491 27 479 6.14e-94 301
MS.gene35341.t1 AT1G67900 37.200 500 272 7 13 486 24 507 5.28e-93 300
MS.gene35341.t1 AT1G67900 37.200 500 272 7 13 486 24 507 5.28e-93 300
MS.gene35341.t1 AT1G67900 37.200 500 272 7 13 486 24 507 5.28e-93 300
MS.gene35341.t1 AT1G67900 37.200 500 272 7 13 486 24 507 5.28e-93 300
MS.gene35341.t1 AT1G67900 37.200 500 272 7 13 486 24 507 5.28e-93 300
MS.gene35341.t1 AT1G67900 37.200 500 272 7 13 486 24 507 5.28e-93 300
MS.gene35341.t1 AT2G47860 31.631 607 350 12 13 591 35 604 6.83e-93 300
MS.gene35341.t1 AT5G10250 30.690 580 329 11 8 561 43 575 5.21e-92 297
MS.gene35341.t1 AT5G67385 33.595 509 306 9 36 541 1 480 9.33e-92 294
MS.gene35341.t1 AT5G67385 33.595 509 306 9 36 541 1 480 9.33e-92 294
MS.gene35341.t1 AT5G67385 33.595 509 306 9 36 541 1 480 9.33e-92 294
MS.gene35341.t1 AT5G67385 33.595 509 306 9 36 541 1 480 9.33e-92 294
MS.gene35341.t1 AT5G66560 36.701 485 233 11 10 438 14 480 1.84e-90 288
MS.gene35341.t1 AT2G30520 34.236 517 297 8 35 550 2 476 1.52e-89 288
MS.gene35341.t1 AT2G30520 34.236 517 297 8 35 550 2 476 1.52e-89 288
MS.gene35341.t1 AT5G10250 30.756 569 328 11 8 561 43 560 3.91e-89 289
MS.gene35341.t1 AT5G47800 34.599 474 281 8 16 486 27 474 6.19e-88 284
MS.gene35341.t1 AT5G47800 34.599 474 281 8 16 486 27 474 6.19e-88 284
MS.gene35341.t1 AT5G47800 34.599 474 281 8 16 486 27 474 6.19e-88 284
MS.gene35341.t1 AT2G47860 30.476 630 350 13 13 591 35 627 1.01e-87 286
MS.gene35341.t1 AT5G67440 36.034 469 262 9 30 491 5 442 1.90e-87 283
MS.gene35341.t1 AT5G47800 34.599 474 281 8 16 486 140 587 3.30e-86 283
MS.gene35341.t1 AT4G31820 32.803 503 290 12 5 499 17 479 4.20e-84 275
MS.gene35341.t1 AT2G23050 33.891 478 252 9 25 495 37 457 3.23e-82 267
MS.gene35341.t1 AT4G37590 34.429 517 277 14 10 514 22 488 8.13e-82 269
MS.gene35341.t1 AT3G26490 31.346 520 299 8 13 500 24 517 4.25e-80 265
MS.gene35341.t1 AT3G26490 31.346 520 299 8 13 500 24 517 4.25e-80 265
MS.gene35341.t1 AT3G26490 31.346 520 299 8 13 500 24 517 4.25e-80 265
MS.gene35341.t1 AT3G49970 29.866 596 318 16 23 600 2 515 4.82e-75 249
MS.gene35341.t1 AT3G49970 29.866 596 318 16 23 600 2 515 4.82e-75 249
MS.gene35341.t1 AT3G08660 33.105 438 234 9 10 439 31 417 2.23e-72 239
MS.gene35341.t1 AT4G31820 32.805 442 251 11 54 487 11 414 1.14e-71 240
MS.gene35341.t1 AT2G47860 29.344 518 306 10 102 591 1 486 1.24e-64 221
MS.gene35341.t1 AT2G47860 29.344 518 306 10 102 591 1 486 1.24e-64 221
MS.gene35341.t1 AT2G47860 31.839 446 246 10 13 437 35 443 6.02e-63 215
MS.gene35341.t1 AT3G26490 29.913 458 265 7 13 438 24 457 1.47e-62 215
MS.gene35341.t1 AT2G47860 30.213 470 247 11 13 438 35 467 5.30e-58 202
MS.gene35341.t1 AT2G23050 30.905 398 214 8 102 495 1 341 1.61e-56 195
MS.gene35341.t1 AT3G19850 28.125 448 272 7 57 487 50 464 1.15e-54 195
MS.gene35341.t1 AT5G17580 27.419 496 300 10 17 500 22 469 1.84e-49 181
MS.gene35341.t1 AT5G17580 27.755 490 294 10 17 494 7 448 2.22e-49 181
MS.gene35341.t1 AT1G52770 31.250 368 219 6 204 551 28 381 3.20e-49 178
MS.gene35341.t1 AT1G50280 27.828 442 273 11 57 482 16 427 1.73e-48 177
MS.gene35341.t1 AT1G50280 27.828 442 273 11 57 482 49 460 3.39e-48 177
MS.gene35341.t1 AT3G22104 26.964 560 316 13 17 562 6 486 4.62e-48 176
MS.gene35341.t1 AT5G48130 24.535 591 374 15 7 578 31 568 8.81e-48 177
MS.gene35341.t1 AT3G03510 28.474 439 228 10 67 494 4 367 2.45e-47 173
MS.gene35341.t1 AT3G15570 34.114 299 166 5 204 489 29 309 3.49e-46 169
MS.gene35341.t1 AT3G22104 26.132 486 269 10 89 562 4 411 3.33e-37 144
MS.gene35341.t1 AT3G22104 26.132 486 269 10 89 562 4 411 3.33e-37 144
MS.gene35341.t1 AT3G22104 26.132 486 269 10 89 562 4 411 3.33e-37 144
MS.gene35341.t1 AT3G49900 30.556 180 104 5 388 557 214 382 2.11e-13 73.6
MS.gene35341.t1 AT3G49900 29.508 183 105 5 388 557 214 385 6.71e-12 68.9

Find 153 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCACCTGGGAAGCTTTC+TGG 0.046977 2.3:-13395691 MS.gene35341:CDS
TTTGGTAGGTTTAGTGATTC+TGG 0.090514 2.3:-13395443 MS.gene35341:intron
ATGGCACCTGCAAGGCTTTC+GGG 0.203178 2.3:+13394029 None:intergenic
GCCACAATCAAGAAAGTTTC+AGG 0.210799 2.3:+13394515 None:intergenic
CGTAGATCAGAGGGTTCTTT+TGG 0.210847 2.3:-13393898 MS.gene35341:CDS
TGCACGGACCCGAGTTTATT+TGG 0.228486 2.3:-13394233 MS.gene35341:CDS
CTCCACCTGGGAAGCTTTCT+GGG 0.231623 2.3:-13395690 MS.gene35341:intron
GTTCTTCTCATAGGTATTCT+TGG 0.237719 2.3:+13394571 None:intergenic
TGCTGTTGTGCGCGAAAATC+AGG 0.238977 2.3:-13393055 MS.gene35341:CDS
ACGAAACCCGTAACAGAATT+TGG 0.245072 2.3:+13394493 None:intergenic
CAACTGGAAAGATTGCATTT+TGG 0.261760 2.3:-13394342 MS.gene35341:CDS
TCAGGGTTTGAAGGTGGATA+TGG 0.270583 2.3:-13393037 MS.gene35341:CDS
GCCCGGGTGGAAGCATTCTT+TGG 0.292191 2.3:-13394178 MS.gene35341:CDS
TCAAGCTTGCCAGGAATCTT+TGG 0.297335 2.3:-13393781 MS.gene35341:CDS
GCCAGGTCTCGGTCGGTGGC+AGG 0.305214 2.3:-13393982 MS.gene35341:CDS
TATGGCACCTGCAAGGCTTT+CGG 0.307178 2.3:+13394028 None:intergenic
TTCTGTGTGTCTGGACTTAC+GGG 0.315632 2.3:+13392795 None:intergenic
ATAGAACGATCGAGGGATTC+AGG 0.338611 2.3:+13392856 None:intergenic
AGACTTGAAGAACAACTCTA+AGG 0.343704 2.3:+13393208 None:intergenic
TTTCTGTGTGTCTGGACTTA+CGG 0.360820 2.3:+13392794 None:intergenic
GTGCCATAATGTATTATTCT+AGG 0.366832 2.3:-13394013 MS.gene35341:CDS
TTGGAACTTGCAACATTAGA+TGG 0.368879 2.3:-13393738 MS.gene35341:CDS
TTCTTCTCTCCAAAGATTCC+TGG 0.369534 2.3:+13393772 None:intergenic
ATAGAATAGGCTCAGAGCTT+TGG 0.373664 2.3:+13394315 None:intergenic
TTGTGGCCAAATTCTGTTAC+GGG 0.375010 2.3:-13394499 MS.gene35341:CDS
TTGGTAGGTTTAGTGATTCT+GGG 0.376444 2.3:-13395442 MS.gene35341:intron
AGCAGGCGTCAAACTAAAAC+TGG 0.383350 2.3:+13393929 None:intergenic
CGTATTCATCCGTCATTTCA+AGG 0.390444 2.3:+13394420 None:intergenic
ATGAGACCCGAAAGCCTTGC+AGG 0.391213 2.3:-13394035 MS.gene35341:CDS
TAAATACCGGCGCTAGGATT+CGG 0.393615 2.3:-13394148 MS.gene35341:CDS
CTTCCTAGAATAATACATTA+TGG 0.393701 2.3:+13394010 None:intergenic
ACCGCGTGCACTGTGAGATT+TGG 0.397256 2.3:+13392942 None:intergenic
TCCTGAAACTTTCTTGATTG+TGG 0.400615 2.3:-13394516 MS.gene35341:CDS
GAATCTTTGGAGAGAAGAAT+AGG 0.402865 2.3:-13393768 MS.gene35341:CDS
GTGGTGCTGGAAGAATTTCC+TGG 0.413339 2.3:-13394542 MS.gene35341:CDS
GAAACTTCTTCCTCAAAAGA+AGG 0.424762 2.3:-13393865 MS.gene35341:CDS
CCACCTCGAGCAGGAACTGC+TGG 0.431873 2.3:+13393114 None:intergenic
AGCTGAAAATCTTCACTTAG+TGG 0.431988 2.3:-13394288 MS.gene35341:CDS
GGTCGGTGGCAGGGTGGACA+AGG 0.437743 2.3:-13393972 MS.gene35341:CDS
GAATGTTGGTCAAGCTTGCC+AGG 0.438919 2.3:-13393790 MS.gene35341:CDS
GAGAAGAATAGGGATGCAAT+TGG 0.439069 2.3:-13393757 MS.gene35341:CDS
GGTTGAGCATCTGAAAATCT+AGG 0.440908 2.3:+13392877 None:intergenic
CATCGTCATCTGAATCGTTA+AGG 0.443490 2.3:-13393581 MS.gene35341:CDS
TGCTTCAAAATGGCTCCACC+TGG 0.443870 2.3:-13395703 None:intergenic
ATTCCGTAAGTACAAAATGA+TGG 0.452056 2.3:+13393649 None:intergenic
CTTGGAATCTTCACAGGTAC+GGG 0.453642 2.3:+13394589 None:intergenic
CGGTTACTTGCAAGAAAACT+CGG 0.454375 2.3:-13392913 MS.gene35341:CDS
CCAGGTCTCGGTCGGTGGCA+GGG 0.455223 2.3:-13393981 MS.gene35341:CDS
CACTGTCAGGTTCTTCTCAT+AGG 0.456166 2.3:+13394562 None:intergenic
CTTTGGAATGGTATAAATAC+CGG 0.458255 2.3:-13394161 MS.gene35341:CDS
AATCTTTGGAGAGAAGAATA+GGG 0.459226 2.3:-13393767 MS.gene35341:CDS
ATTGTGGCCAAATTCTGTTA+CGG 0.459464 2.3:-13394500 MS.gene35341:CDS
AGCCGGATGTGTCAATGCTT+TGG 0.460297 2.3:-13393151 MS.gene35341:CDS
CTCATGCATCTCAAAATGAT+AGG 0.461872 2.3:-13393236 MS.gene35341:CDS
AGGAAGCACTTGCCAGGTCT+CGG 0.466707 2.3:-13393993 MS.gene35341:CDS
AAGTTGATAGATAGCTATAT+TGG 0.467102 2.3:-13393549 MS.gene35341:CDS
GATATTACCGTGTCAATTGA+TGG 0.469085 2.3:-13395410 MS.gene35341:CDS
TTTCCTCTTGTTTCAAGATG+TGG 0.472009 2.3:-13394620 MS.gene35341:CDS
GGATGGCCAATGATGATGTA+TGG 0.474163 2.3:-13394212 MS.gene35341:CDS
AGCACTGGACATATATTTCA+AGG 0.477136 2.3:-13393427 MS.gene35341:intron
TATTCTAGGAAGCACTTGCC+AGG 0.478232 2.3:-13393999 MS.gene35341:CDS
ATTCTTCCAGCACCACTGTC+AGG 0.483228 2.3:+13394549 None:intergenic
ACACTTTGCAGGCTCATCCT+TGG 0.485150 2.3:-13393329 MS.gene35341:intron
CGGACCCGAGTTTATTTGGA+TGG 0.488402 2.3:-13394229 MS.gene35341:CDS
CGGGTCCGTGCAAGCCATCA+TGG 0.490014 2.3:+13394244 None:intergenic
TGAAACCCAGAAAGCTTCCC+AGG 0.493717 2.3:+13395685 None:intergenic
GCTCGCTCAACTGATGCTCT+TGG 0.496197 2.3:+13392826 None:intergenic
CCCTGCCACCGACCGAGACC+TGG 0.506361 2.3:+13393981 None:intergenic
AAACTCCCATACATCATCAT+TGG 0.511982 2.3:+13394206 None:intergenic
ATCAAGCGATGAAGGAGAAA+AGG 0.512046 2.3:+13393614 None:intergenic
TCTTGGAATCTTCACAGGTA+CGG 0.512815 2.3:+13394588 None:intergenic
GCATCAGAATCTGCATAAGC+AGG 0.514844 2.3:+13393705 None:intergenic
ATCCAAAGCATTGACACATC+CGG 0.515020 2.3:+13393149 None:intergenic
GAAGAACCTGACAGTGGTGC+TGG 0.515078 2.3:-13394555 MS.gene35341:CDS
GACTTGAAGAACAACTCTAA+GGG 0.520796 2.3:+13393209 None:intergenic
TGGTGCTGGAAGAATTTCCT+GGG 0.521506 2.3:-13394541 MS.gene35341:CDS
CCTGCTCGAGGTGGTACGGC+TGG 0.521573 2.3:-13393105 MS.gene35341:CDS
CATTTGAGAACAGCATTAGC+CGG 0.524224 2.3:-13393168 MS.gene35341:CDS
TGACGGACTCTACCGAGCAC+TGG 0.526424 2.3:-13393442 MS.gene35341:CDS
TGATCTACGGTAGCACTAGC+AGG 0.526842 2.3:+13393912 None:intergenic
GGTATTCTTGGAATCTTCAC+AGG 0.528174 2.3:+13394583 None:intergenic
GCTTGCGGATCAAGCGATGA+AGG 0.528515 2.3:+13393606 None:intergenic
AAGTGCATACCTTGTGAAGA+TGG 0.532440 2.3:+13395378 None:intergenic
TTTAAACGCTCTATCCATGA+TGG 0.533219 2.3:-13394258 MS.gene35341:CDS
ACTTGCCAGGTCTCGGTCGG+TGG 0.536865 2.3:-13393986 MS.gene35341:CDS
CAAAAGAACCCTCTGATCTA+CGG 0.537722 2.3:+13393899 None:intergenic
TTTCCACATCTTGAAACAAG+AGG 0.538173 2.3:+13394617 None:intergenic
ACAGTGCACGCGGTTCTCCT+CGG 0.546364 2.3:-13392933 MS.gene35341:CDS
GCGCGAAAATCAGGGTTTGA+AGG 0.547646 2.3:-13393046 MS.gene35341:CDS
TTCCAAAGAATGCTTCCACC+CGG 0.549351 2.3:+13394176 None:intergenic
AAACTTCTTCCTCAAAAGAA+GGG 0.557294 2.3:-13393864 MS.gene35341:CDS
AGTTCCTGCTCGAGGTGGTA+CGG 0.557664 2.3:-13393109 MS.gene35341:CDS
GGTGGAAGCATTCTTTGGAA+TGG 0.559847 2.3:-13394173 MS.gene35341:CDS
GCTGTTGTGCGCGAAAATCA+GGG 0.560005 2.3:-13393054 MS.gene35341:CDS
TCCAAAGAATGCTTCCACCC+GGG 0.565201 2.3:+13394177 None:intergenic
ATGCAATGATCTCGATGACT+CGG 0.566672 2.3:+13393464 None:intergenic
AAACCACCACTCGGATTCTG+AGG 0.569410 2.3:+13394124 None:intergenic
ATGATCTCGATGACTCGGGA+AGG 0.569668 2.3:+13393469 None:intergenic
TCTATCCATGATGGCTTGCA+CGG 0.573224 2.3:-13394249 MS.gene35341:CDS
CTCAACTGATGCTCTTGGTG+TGG 0.573680 2.3:+13392831 None:intergenic
ATGGTATAAATACCGGCGCT+AGG 0.573781 2.3:-13394154 MS.gene35341:CDS
TGCAATGATCTCGATGACTC+GGG 0.575045 2.3:+13393465 None:intergenic
CGAAAATCAGGGTTTGAAGG+TGG 0.575204 2.3:-13393043 MS.gene35341:CDS
GAAGAAGTCCGAGTAAGAAA+CGG 0.583295 2.3:+13393829 None:intergenic
TGATCTCGATGACTCGGGAA+GGG 0.585868 2.3:+13393470 None:intergenic
AGCACTTGCCAGGTCTCGGT+CGG 0.588649 2.3:-13393989 MS.gene35341:CDS
ATGACGGATGAATACGAAGA+AGG 0.589120 2.3:-13394413 MS.gene35341:CDS
AATTGTACAAAGAGACGGAT+GGG 0.589847 2.3:-13393079 MS.gene35341:CDS
CGGTGGCAGGGTGGACAAGG+TGG 0.597233 2.3:-13393969 MS.gene35341:CDS
CTAGTGCTACCGTAGATCAG+AGG 0.598598 2.3:-13393908 MS.gene35341:CDS
TTCTCGTTATCAGAGAGCCA+AGG 0.599221 2.3:+13393312 None:intergenic
ATTGGCCATCCAAATAAACT+CGG 0.599515 2.3:+13394224 None:intergenic
GGTCTCGGTCGGTGGCAGGG+TGG 0.603022 2.3:-13393978 MS.gene35341:CDS
GCTTCAAAATGGCTCCACCT+GGG 0.603801 2.3:-13395702 None:intergenic
GAGATGCATGAGCACATGCA+TGG 0.604027 2.3:+13393247 None:intergenic
TCGCTCTCATTGTCTTAACA+AGG 0.604494 2.3:+13394063 None:intergenic
TGAAATATATGTCCAGTGCT+CGG 0.604995 2.3:+13393430 None:intergenic
GTAGCTTCGATATTGAATGT+TGG 0.608242 2.3:-13393804 MS.gene35341:CDS
TGCACTGTGAGATTTGGTCA+CGG 0.613993 2.3:+13392948 None:intergenic
GCTCCAGCAGTTCCTGCTCG+AGG 0.616130 2.3:-13393117 MS.gene35341:CDS
CCAGCCGTACCACCTCGAGC+AGG 0.617229 2.3:+13393105 None:intergenic
ATTGACACGGTAATATCACT+TGG 0.619878 2.3:+13395416 None:intergenic
GATAGAATGAGTACTAGAGT+CGG 0.620497 2.3:-13393015 MS.gene35341:CDS
GATGGCCAATGATGATGTAT+GGG 0.623127 2.3:-13394211 MS.gene35341:CDS
GGAGAAATTGTACAAAGAGA+CGG 0.628527 2.3:-13393084 MS.gene35341:CDS
GGATGTGTCAATGCTTTGGA+TGG 0.630355 2.3:-13393147 MS.gene35341:CDS
GTGTGGATATAGAACGATCG+AGG 0.630737 2.3:+13392848 None:intergenic
TAGTGCTACCGTAGATCAGA+GGG 0.631634 2.3:-13393907 MS.gene35341:CDS
GGACCTCAGAATCCGAGTGG+TGG 0.632921 2.3:-13394127 MS.gene35341:CDS
GGTGCTGGAAGAATTTCCTG+GGG 0.637441 2.3:-13394540 MS.gene35341:CDS
ACTTGAAGAACAACTCTAAG+GGG 0.637979 2.3:+13393210 None:intergenic
CCAGCAGTTCCTGCTCGAGG+TGG 0.638189 2.3:-13393114 MS.gene35341:CDS
TGAGGTCCGAATCCTAGCGC+CGG 0.638429 2.3:+13394142 None:intergenic
TGCAGAATACCTTGAAATGA+CGG 0.639662 2.3:-13394429 MS.gene35341:CDS
TCAGATGACGATGATGCTTG+CGG 0.640050 2.3:+13393591 None:intergenic
GATGTCTTCAAACCACCACT+CGG 0.642289 2.3:+13394115 None:intergenic
AAATTGTACAAAGAGACGGA+TGG 0.644116 2.3:-13393080 MS.gene35341:CDS
AATACATTATGGCACCTGCA+AGG 0.648443 2.3:+13394021 None:intergenic
GGTTACTTGCAAGAAAACTC+GGG 0.650025 2.3:-13392912 MS.gene35341:CDS
TGTGTCTGGACTTACGGGAA+CGG 0.650765 2.3:+13392800 None:intergenic
GTTAAGACAATGAGAGCGAG+AGG 0.652121 2.3:-13394059 MS.gene35341:CDS
TTGGCCATCCAAATAAACTC+GGG 0.657524 2.3:+13394225 None:intergenic
TGTCAATTTCCATCTTCACA+AGG 0.664191 2.3:-13395387 MS.gene35341:intron
ACCAAATCTCACAGTGCACG+CGG 0.665622 2.3:-13392943 MS.gene35341:CDS
AACCCAGAAAGCTTCCCAGG+TGG 0.686788 2.3:+13395688 None:intergenic
ATTGACACCATCAATTGACA+CGG 0.690615 2.3:+13395403 None:intergenic
TGTGGATATAGAACGATCGA+GGG 0.711973 2.3:+13392849 None:intergenic
GTGCTGGAAGAATTTCCTGG+GGG 0.715345 2.3:-13394539 MS.gene35341:CDS
AAGCGATGAAGGAGAAAAGG+CGG 0.715407 2.3:+13393617 None:intergenic
TCATCGAGATCATTGCATGA+CGG 0.715773 2.3:-13393459 MS.gene35341:CDS
TTCGGACCTCAGAATCCGAG+TGG 0.719040 2.3:-13394130 MS.gene35341:CDS
CAAGGTATTCTGCAGCACAG+TGG 0.723725 2.3:+13394438 None:intergenic
CTATGAGAAGAACCTGACAG+TGG 0.738065 2.3:-13394561 MS.gene35341:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTCTTTATTATTTATTTAT+TGG - chr2.3:13392858-13392877 MS.gene35341:CDS 10.0%
!! AAATAATCTAACAGTAAAAT+AGG + chr2.3:13393292-13393311 None:intergenic 15.0%
!! AGAATTCAGTAAAATAAAAA+AGG - chr2.3:13395497-13395516 MS.gene35341:intron 15.0%
!! TATATTGGAGAAAATTTATT+TGG - chr2.3:13393652-13393671 MS.gene35341:CDS 15.0%
!! TATTCATAATAATTTGATAG+TGG - chr2.3:13393159-13393178 MS.gene35341:CDS 15.0%
!!! AAAACTTTTTTACATCAATA+GGG + chr2.3:13393323-13393342 None:intergenic 15.0%
!!! ATTTTTTTTTCCTGTTTTTT+TGG - chr2.3:13393003-13393022 MS.gene35341:CDS 15.0%
!!! TAAAACTTTTTTACATCAAT+AGG + chr2.3:13393324-13393343 None:intergenic 15.0%
!! AAACAAAATTCCAAAAAAAC+AGG + chr2.3:13393016-13393035 None:intergenic 20.0%
!! ACAAAAGAACATAAATGAAT+AGG + chr2.3:13393226-13393245 None:intergenic 20.0%
!! GAAAAGAAACAATTATGAAA+TGG + chr2.3:13393125-13393144 None:intergenic 20.0%
!!! ACTAAATGCATTTAAAATGT+TGG + chr2.3:13393568-13393587 None:intergenic 20.0%
!!! AGGTGAATATTTTGATATAT+TGG - chr2.3:13393637-13393656 MS.gene35341:CDS 20.0%
!!! GTTTTTTTGGAATTTTGTTT+TGG - chr2.3:13393016-13393035 MS.gene35341:CDS 20.0%
!!! TATTATGAATAGAGTATTAG+TGG + chr2.3:13393150-13393169 None:intergenic 20.0%
! AATTACTTGTAATGTTGTGT+TGG - chr2.3:13393803-13393822 MS.gene35341:CDS 25.0%
! ATAAAAATATATTGCGCAAC+TGG - chr2.3:13394119-13394138 MS.gene35341:CDS 25.0%
! CTTCCTAGAATAATACATTA+TGG + chr2.3:13394470-13394489 None:intergenic 25.0%
!! TTTTGTGAAACATAATTGCA+TGG - chr2.3:13393741-13393760 MS.gene35341:CDS 25.0%
!!! AAGTTGATAGATAGCTATAT+TGG - chr2.3:13394928-13394947 MS.gene35341:intron 25.0%
!!! AGTTGCGCAATATATTTTTA+TGG + chr2.3:13394120-13394139 None:intergenic 25.0%
!!! GAAAATGACATGTTTTTTAG+TGG - chr2.3:13392926-13392945 MS.gene35341:CDS 25.0%
!!! TTTTGGAATTTTGTTTTGGT+AGG - chr2.3:13393020-13393039 MS.gene35341:CDS 25.0%
AAACTTCTTCCTCAAAAGAA+GGG - chr2.3:13394613-13394632 MS.gene35341:CDS 30.0%
AGTAAAATAGGTCACAAAGT+AGG + chr2.3:13393280-13393299 None:intergenic 30.0%
ATAATTGCATGGTGAAATAG+GGG - chr2.3:13393714-13393733 MS.gene35341:CDS 30.0%
ATTCCGTAAGTACAAAATGA+TGG + chr2.3:13394831-13394850 None:intergenic 30.0%
ATTGATTGAAAATGTAGCTC+AGG + chr2.3:13395084-13395103 None:intergenic 30.0%
CATAATTGCATGGTGAAATA+GGG - chr2.3:13393713-13393732 MS.gene35341:CDS 30.0%
GAGAAAATTTATTTGGAAGC+TGG - chr2.3:13393659-13393678 MS.gene35341:CDS 30.0%
GTAAAATAGGTCACAAAGTA+GGG + chr2.3:13393279-13393298 None:intergenic 30.0%
GTGCCATAATGTATTATTCT+AGG - chr2.3:13394464-13394483 MS.gene35341:CDS 30.0%
GTTATGTGGCTACAATTAAA+AGG - chr2.3:13393611-13393630 MS.gene35341:CDS 30.0%
TAAAATAGGTCACAAAGTAG+GGG + chr2.3:13393278-13393297 None:intergenic 30.0%
TATAAAGTCACACGTATGAT+TGG + chr2.3:13393366-13393385 None:intergenic 30.0%
TATTTCACCATGCAATTATG+CGG + chr2.3:13393713-13393732 None:intergenic 30.0%
TGGCTACAATTAAAAGGAAA+AGG - chr2.3:13393617-13393636 MS.gene35341:CDS 30.0%
TGTGATCTCTAGTTAATGAA+GGG + chr2.3:13393478-13393497 None:intergenic 30.0%
! AAAGATTTTCCCTTCTTTTG+AGG + chr2.3:13394625-13394644 None:intergenic 30.0%
! AATCTTTGGAGAGAAGAATA+GGG - chr2.3:13394710-13394729 MS.gene35341:intron 30.0%
! TGTCCATCATTTTGTACTTA+CGG - chr2.3:13394825-13394844 MS.gene35341:intron 30.0%
! TTTTCAGCTCTTGATAGAAT+AGG + chr2.3:13394178-13394197 None:intergenic 30.0%
!! ATAATTTGATAGTGGAGTAG+TGG - chr2.3:13393167-13393186 MS.gene35341:CDS 30.0%
!! CTTTGGAATGGTATAAATAC+CGG - chr2.3:13394316-13394335 MS.gene35341:CDS 30.0%
!! TTTTTGACTCTTTACATCTG+CGG - chr2.3:13393410-13393429 MS.gene35341:intron 30.0%
!!! GTTGAGATTAGTTTCTTTTG+TGG - chr2.3:13393189-13393208 MS.gene35341:CDS 30.0%
AAACTCCCATACATCATCAT+TGG + chr2.3:13394274-13394293 None:intergenic 35.0%
AAATTGTACAAAGAGACGGA+TGG - chr2.3:13395397-13395416 MS.gene35341:CDS 35.0%
AATTGTACAAAGAGACGGAT+GGG - chr2.3:13395398-13395417 MS.gene35341:CDS 35.0%
ACTTGAAGAACAACTCTAAG+GGG + chr2.3:13395270-13395289 None:intergenic 35.0%
AGAAAAACTTTCTGTGTGTC+TGG + chr2.3:13395694-13395713 None:intergenic 35.0%
AGACTTGAAGAACAACTCTA+AGG + chr2.3:13395272-13395291 None:intergenic 35.0%
AGCTGAAAATCTTCACTTAG+TGG - chr2.3:13394189-13394208 MS.gene35341:CDS 35.0%
AGTTGCATGTATGTATGACT+TGG + chr2.3:13392955-13392974 None:intergenic 35.0%
ATTGACACCATCAATTGACA+CGG + chr2.3:13393077-13393096 None:intergenic 35.0%
ATTGACACGGTAATATCACT+TGG + chr2.3:13393064-13393083 None:intergenic 35.0%
ATTGGCCATCCAAATAAACT+CGG + chr2.3:13394256-13394275 None:intergenic 35.0%
ATTGTGGCCAAATTCTGTTA+CGG - chr2.3:13393977-13393996 MS.gene35341:CDS 35.0%
ATTTCACCATGCAATTATGC+GGG + chr2.3:13393712-13393731 None:intergenic 35.0%
CTCATGCATCTCAAAATGAT+AGG - chr2.3:13395241-13395260 MS.gene35341:intron 35.0%
GAAACTTCTTCCTCAAAAGA+AGG - chr2.3:13394612-13394631 MS.gene35341:CDS 35.0%
GACTTGAAGAACAACTCTAA+GGG + chr2.3:13395271-13395290 None:intergenic 35.0%
GATAGAATGAGTACTAGAGT+CGG - chr2.3:13395462-13395481 MS.gene35341:intron 35.0%
GATATTACCGTGTCAATTGA+TGG - chr2.3:13393067-13393086 MS.gene35341:CDS 35.0%
GCATAATTGCATGGTGAAAT+AGG - chr2.3:13393712-13393731 MS.gene35341:CDS 35.0%
GGAGAAATTGTACAAAGAGA+CGG - chr2.3:13395393-13395412 MS.gene35341:CDS 35.0%
GTAGCTTCGATATTGAATGT+TGG - chr2.3:13394673-13394692 MS.gene35341:intron 35.0%
GTGTGATCTCTAGTTAATGA+AGG + chr2.3:13393479-13393498 None:intergenic 35.0%
GTTCTTCTCATAGGTATTCT+TGG + chr2.3:13393909-13393928 None:intergenic 35.0%
TAATTGCATGGTGAAATAGG+GGG - chr2.3:13393715-13393734 MS.gene35341:CDS 35.0%
TCCTGAAACTTTCTTGATTG+TGG - chr2.3:13393961-13393980 MS.gene35341:CDS 35.0%
TGAAATATATGTCCAGTGCT+CGG + chr2.3:13395050-13395069 None:intergenic 35.0%
TGCAGAATACCTTGAAATGA+CGG - chr2.3:13394048-13394067 MS.gene35341:CDS 35.0%
TGTCAATTTCCATCTTCACA+AGG - chr2.3:13393090-13393109 MS.gene35341:CDS 35.0%
TTGGAACTTGCAACATTAGA+TGG - chr2.3:13394739-13394758 MS.gene35341:intron 35.0%
TTGGTAGGTTTAGTGATTCT+GGG - chr2.3:13393035-13393054 MS.gene35341:CDS 35.0%
TTTAAACGCTCTATCCATGA+TGG - chr2.3:13394219-13394238 MS.gene35341:CDS 35.0%
TTTCCACATCTTGAAACAAG+AGG + chr2.3:13393863-13393882 None:intergenic 35.0%
TTTGGTAGGTTTAGTGATTC+TGG - chr2.3:13393034-13393053 MS.gene35341:CDS 35.0%
! AGCACTGGACATATATTTCA+AGG - chr2.3:13395050-13395069 MS.gene35341:intron 35.0%
! CAACTGGAAAGATTGCATTT+TGG - chr2.3:13394135-13394154 MS.gene35341:CDS 35.0%
! GAATCTTTGGAGAGAAGAAT+AGG - chr2.3:13394709-13394728 MS.gene35341:intron 35.0%
! GTTCACTTGATCTACTGTTT+TGG - chr2.3:13393586-13393605 MS.gene35341:CDS 35.0%
! TTTCCTCTTGTTTCAAGATG+TGG - chr2.3:13393857-13393876 MS.gene35341:CDS 35.0%
!! ATGCATGGATTGAGAGTTTT+TGG + chr2.3:13395218-13395237 None:intergenic 35.0%
!! TCTTTTTGCCGTTTCTTACT+CGG - chr2.3:13394640-13394659 MS.gene35341:intron 35.0%
!!! CTACTGTTTTGGAAGTTATG+TGG - chr2.3:13393597-13393616 MS.gene35341:CDS 35.0%
AAGTGCATACCTTGTGAAGA+TGG + chr2.3:13393102-13393121 None:intergenic 40.0%
AATACATTATGGCACCTGCA+AGG + chr2.3:13394459-13394478 None:intergenic 40.0%
ACGAAACCCGTAACAGAATT+TGG + chr2.3:13393987-13394006 None:intergenic 40.0%
ATAGAATAGGCTCAGAGCTT+TGG + chr2.3:13394165-13394184 None:intergenic 40.0%
ATCAAGCGATGAAGGAGAAA+AGG + chr2.3:13394866-13394885 None:intergenic 40.0%
ATGCAATGATCTCGATGACT+CGG + chr2.3:13395016-13395035 None:intergenic 40.0%
CAAAAGAACCCTCTGATCTA+CGG + chr2.3:13394581-13394600 None:intergenic 40.0%
CATCGTCATCTGAATCGTTA+AGG - chr2.3:13394896-13394915 MS.gene35341:intron 40.0%
CATTTGAGAACAGCATTAGC+CGG - chr2.3:13395309-13395328 MS.gene35341:intron 40.0%
CGGTTACTTGCAAGAAAACT+CGG - chr2.3:13395564-13395583 MS.gene35341:intron 40.0%
CGTATTCATCCGTCATTTCA+AGG + chr2.3:13394060-13394079 None:intergenic 40.0%
CTTTCTGGGTTTCATCTTCA+AGG - chr2.3:13392801-13392820 MS.gene35341:CDS 40.0%
GAAGAAGTCCGAGTAAGAAA+CGG + chr2.3:13394651-13394670 None:intergenic 40.0%
GAGAAGAATAGGGATGCAAT+TGG - chr2.3:13394720-13394739 MS.gene35341:intron 40.0%
GCCACAATCAAGAAAGTTTC+AGG + chr2.3:13393965-13393984 None:intergenic 40.0%
GGTATTCTTGGAATCTTCAC+AGG + chr2.3:13393897-13393916 None:intergenic 40.0%
GGTTACTTGCAAGAAAACTC+GGG - chr2.3:13395565-13395584 MS.gene35341:intron 40.0%
GGTTGAGCATCTGAAAATCT+AGG + chr2.3:13395603-13395622 None:intergenic 40.0%
GTTGGAGTCTGAGTAAGATA+TGG - chr2.3:13393821-13393840 MS.gene35341:CDS 40.0%
TCATCGAGATCATTGCATGA+CGG - chr2.3:13395018-13395037 MS.gene35341:intron 40.0%
TCGCTCTCATTGTCTTAACA+AGG + chr2.3:13394417-13394436 None:intergenic 40.0%
TCTTGGAATCTTCACAGGTA+CGG + chr2.3:13393892-13393911 None:intergenic 40.0%
TGAATTCCCGCATAATTGCA+TGG - chr2.3:13393703-13393722 MS.gene35341:CDS 40.0%
TGTGGATATAGAACGATCGA+GGG + chr2.3:13395631-13395650 None:intergenic 40.0%
TTCATCTTCAAGGTCATCAC+TGG - chr2.3:13392811-13392830 MS.gene35341:CDS 40.0%
TTCTTCTCTCCAAAGATTCC+TGG + chr2.3:13394708-13394727 None:intergenic 40.0%
TTGGCCATCCAAATAAACTC+GGG + chr2.3:13394255-13394274 None:intergenic 40.0%
TTGTGGCCAAATTCTGTTAC+GGG - chr2.3:13393978-13393997 MS.gene35341:CDS 40.0%
TTTCTGTGTGTCTGGACTTA+CGG + chr2.3:13395686-13395705 None:intergenic 40.0%
! ACACGTATGATTGGTTGTGA+AGG + chr2.3:13393357-13393376 None:intergenic 40.0%
! ACGTATGATTGGTTGTGAAG+GGG + chr2.3:13393355-13393374 None:intergenic 40.0%
! AGTTTTCTTGCAAGTAACCG+AGG + chr2.3:13395564-13395583 None:intergenic 40.0%
! CAAAGTAGGGGCACATTAAT+TGG + chr2.3:13393266-13393285 None:intergenic 40.0%
! CACGTATGATTGGTTGTGAA+GGG + chr2.3:13393356-13393375 None:intergenic 40.0%
! GATGGCCAATGATGATGTAT+GGG - chr2.3:13394266-13394285 MS.gene35341:CDS 40.0%
! TAGGGGCACATTAATTGGAT+TGG + chr2.3:13393261-13393280 None:intergenic 40.0%
!! ATCCAAAGCATTGACACATC+CGG + chr2.3:13395331-13395350 None:intergenic 40.0%
!! ATGACGGATGAATACGAAGA+AGG - chr2.3:13394064-13394083 MS.gene35341:CDS 40.0%
AAGCGATGAAGGAGAAAAGG+CGG + chr2.3:13394863-13394882 None:intergenic 45.0%
AGCAGGCGTCAAACTAAAAC+TGG + chr2.3:13394551-13394570 None:intergenic 45.0%
ATAGAACGATCGAGGGATTC+AGG + chr2.3:13395624-13395643 None:intergenic 45.0%
ATGGTATAAATACCGGCGCT+AGG - chr2.3:13394323-13394342 MS.gene35341:CDS 45.0%
CACTGTCAGGTTCTTCTCAT+AGG + chr2.3:13393918-13393937 None:intergenic 45.0%
CGAAAATCAGGGTTTGAAGG+TGG - chr2.3:13395434-13395453 MS.gene35341:CDS 45.0%
CTATGAGAAGAACCTGACAG+TGG - chr2.3:13393916-13393935 MS.gene35341:CDS 45.0%
CTTGGAATCTTCACAGGTAC+GGG + chr2.3:13393891-13393910 None:intergenic 45.0%
GAATGCTTACGACACTTTGC+AGG - chr2.3:13395137-13395156 MS.gene35341:intron 45.0%
GATGTCTTCAAACCACCACT+CGG + chr2.3:13394365-13394384 None:intergenic 45.0%
GCATCAGAATCTGCATAAGC+AGG + chr2.3:13394775-13394794 None:intergenic 45.0%
GTGTGGATATAGAACGATCG+AGG + chr2.3:13395632-13395651 None:intergenic 45.0%
GTTAAGACAATGAGAGCGAG+AGG - chr2.3:13394418-13394437 MS.gene35341:CDS 45.0%
TAAATACCGGCGCTAGGATT+CGG - chr2.3:13394329-13394348 MS.gene35341:CDS 45.0%
TAGTGCTACCGTAGATCAGA+GGG - chr2.3:13394570-13394589 MS.gene35341:CDS 45.0%
TCAAGCTTGCCAGGAATCTT+TGG - chr2.3:13394696-13394715 MS.gene35341:intron 45.0%
TCAGATGACGATGATGCTTG+CGG + chr2.3:13394889-13394908 None:intergenic 45.0%
TCAGGGTTTGAAGGTGGATA+TGG - chr2.3:13395440-13395459 MS.gene35341:intron 45.0%
TGCAATGATCTCGATGACTC+GGG + chr2.3:13395015-13395034 None:intergenic 45.0%
TTCCAAAGAATGCTTCCACC+CGG + chr2.3:13394304-13394323 None:intergenic 45.0%
TTCTCGTTATCAGAGAGCCA+AGG + chr2.3:13395168-13395187 None:intergenic 45.0%
TTCTGTGTGTCTGGACTTAC+GGG + chr2.3:13395685-13395704 None:intergenic 45.0%
! CGTAGATCAGAGGGTTCTTT+TGG - chr2.3:13394579-13394598 MS.gene35341:CDS 45.0%
! GGATGGCCAATGATGATGTA+TGG - chr2.3:13394265-13394284 MS.gene35341:CDS 45.0%
! GGATGTGTCAATGCTTTGGA+TGG - chr2.3:13395330-13395349 MS.gene35341:intron 45.0%
! TCTATCCATGATGGCTTGCA+CGG - chr2.3:13394228-13394247 MS.gene35341:CDS 45.0%
! TGCACTGTGAGATTTGGTCA+CGG + chr2.3:13395532-13395551 None:intergenic 45.0%
! TGGTGCTGGAAGAATTTCCT+GGG - chr2.3:13393936-13393955 MS.gene35341:CDS 45.0%
!! GGTGGAAGCATTCTTTGGAA+TGG - chr2.3:13394304-13394323 MS.gene35341:CDS 45.0%
!! TATTCTAGGAAGCACTTGCC+AGG - chr2.3:13394478-13394497 MS.gene35341:CDS 45.0%
AAACCACCACTCGGATTCTG+AGG + chr2.3:13394356-13394375 None:intergenic 50.0%
AAGAAAGTTTCAGGACCCCC+AGG + chr2.3:13393956-13393975 None:intergenic 50.0%
ACACTTTGCAGGCTCATCCT+TGG - chr2.3:13395148-13395167 MS.gene35341:intron 50.0%
ACCAAATCTCACAGTGCACG+CGG - chr2.3:13395534-13395553 MS.gene35341:intron 50.0%
AGCCGGATGTGTCAATGCTT+TGG - chr2.3:13395326-13395345 MS.gene35341:intron 50.0%
ATTCTTCCAGCACCACTGTC+AGG + chr2.3:13393931-13393950 None:intergenic 50.0%
CGGACCCGAGTTTATTTGGA+TGG - chr2.3:13394248-13394267 MS.gene35341:CDS 50.0%
CTAGTGCTACCGTAGATCAG+AGG - chr2.3:13394569-13394588 MS.gene35341:CDS 50.0%
CTCAACTGATGCTCTTGGTG+TGG + chr2.3:13395649-13395668 None:intergenic 50.0%
GAATGTTGGTCAAGCTTGCC+AGG - chr2.3:13394687-13394706 MS.gene35341:intron 50.0%
GAGATGCATGAGCACATGCA+TGG + chr2.3:13395233-13395252 None:intergenic 50.0%
GCGCGAAAATCAGGGTTTGA+AGG - chr2.3:13395431-13395450 MS.gene35341:CDS 50.0%
GCTGTTGTGCGCGAAAATCA+GGG - chr2.3:13395423-13395442 MS.gene35341:CDS 50.0%
GTGCTGGAAGAATTTCCTGG+GGG - chr2.3:13393938-13393957 MS.gene35341:CDS 50.0%
TATGGCACCTGCAAGGCTTT+CGG + chr2.3:13394452-13394471 None:intergenic 50.0%
TCCAAAGAATGCTTCCACCC+GGG + chr2.3:13394303-13394322 None:intergenic 50.0%
TGAAACCCAGAAAGCTTCCC+AGG + chr2.3:13392795-13392814 None:intergenic 50.0%
TGATCTACGGTAGCACTAGC+AGG + chr2.3:13394568-13394587 None:intergenic 50.0%
TGCACGGACCCGAGTTTATT+TGG - chr2.3:13394244-13394263 MS.gene35341:CDS 50.0%
TGCTGTTGTGCGCGAAAATC+AGG - chr2.3:13395422-13395441 MS.gene35341:CDS 50.0%
TGTGTCTGGACTTACGGGAA+CGG + chr2.3:13395680-13395699 None:intergenic 50.0%
! ATGATCTCGATGACTCGGGA+AGG + chr2.3:13395011-13395030 None:intergenic 50.0%
! CAAGGTATTCTGCAGCACAG+TGG + chr2.3:13394042-13394061 None:intergenic 50.0%
! GGTGCTGGAAGAATTTCCTG+GGG - chr2.3:13393937-13393956 MS.gene35341:CDS 50.0%
! GTATGGGAGTTTTCAGAGCC+CGG - chr2.3:13394282-13394301 MS.gene35341:CDS 50.0%
! GTGGTGCTGGAAGAATTTCC+TGG - chr2.3:13393935-13393954 MS.gene35341:CDS 50.0%
! TATGGGAGTTTTCAGAGCCC+GGG - chr2.3:13394283-13394302 MS.gene35341:CDS 50.0%
! TGATCTCGATGACTCGGGAA+GGG + chr2.3:13395010-13395029 None:intergenic 50.0%
!! GCAAGAGCACGCAACTTTTC+TGG + chr2.3:13394982-13395001 None:intergenic 50.0%
AACCCAGAAAGCTTCCCAGG+TGG + chr2.3:13392792-13392811 None:intergenic 55.0%
ACCGCGTGCACTGTGAGATT+TGG + chr2.3:13395538-13395557 None:intergenic 55.0%
AGTTCCTGCTCGAGGTGGTA+CGG - chr2.3:13395368-13395387 MS.gene35341:intron 55.0%
ATGAGACCCGAAAGCCTTGC+AGG - chr2.3:13394442-13394461 MS.gene35341:CDS 55.0%
ATGGCACCTGCAAGGCTTTC+GGG + chr2.3:13394451-13394470 None:intergenic 55.0%
CTCCACCTGGGAAGCTTTCT+GGG - chr2.3:13392787-13392806 MS.gene35341:CDS 55.0%
GAAGAACCTGACAGTGGTGC+TGG - chr2.3:13393922-13393941 MS.gene35341:CDS 55.0%
GCTCGCTCAACTGATGCTCT+TGG + chr2.3:13395654-13395673 None:intergenic 55.0%
GCTTGCGGATCAAGCGATGA+AGG + chr2.3:13394874-13394893 None:intergenic 55.0%
TTCGGACCTCAGAATCCGAG+TGG - chr2.3:13394347-13394366 MS.gene35341:CDS 55.0%
!! AGGAAGCACTTGCCAGGTCT+CGG - chr2.3:13394484-13394503 MS.gene35341:CDS 55.0%
ACAGTGCACGCGGTTCTCCT+CGG - chr2.3:13395544-13395563 MS.gene35341:intron 60.0%
AGCACTTGCCAGGTCTCGGT+CGG - chr2.3:13394488-13394507 MS.gene35341:CDS 60.0%
GCTCCACCTGGGAAGCTTTC+TGG - chr2.3:13392786-13392805 MS.gene35341:CDS 60.0%
GGACCTCAGAATCCGAGTGG+TGG - chr2.3:13394350-13394369 MS.gene35341:CDS 60.0%
TGAGGTCCGAATCCTAGCGC+CGG + chr2.3:13394338-13394357 None:intergenic 60.0%
!! GCCCGGGTGGAAGCATTCTT+TGG - chr2.3:13394299-13394318 MS.gene35341:CDS 60.0%
!! TGACGGACTCTACCGAGCAC+TGG - chr2.3:13395035-13395054 MS.gene35341:intron 60.0%
ACTTGCCAGGTCTCGGTCGG+TGG - chr2.3:13394491-13394510 MS.gene35341:CDS 65.0%
CCACCTCGAGCAGGAACTGC+TGG + chr2.3:13395366-13395385 None:intergenic 65.0%
CCAGCAGTTCCTGCTCGAGG+TGG - chr2.3:13395363-13395382 MS.gene35341:intron 65.0%
CGGGTCCGTGCAAGCCATCA+TGG + chr2.3:13394236-13394255 None:intergenic 65.0%
GCTCCAGCAGTTCCTGCTCG+AGG - chr2.3:13395360-13395379 MS.gene35341:intron 65.0%
! GGGAGTTTTCAGAGCCCGGG+TGG - chr2.3:13394286-13394305 MS.gene35341:CDS 65.0%
CCAGCCGTACCACCTCGAGC+AGG + chr2.3:13395375-13395394 None:intergenic 70.0%
CCAGGTCTCGGTCGGTGGCA+GGG - chr2.3:13394496-13394515 MS.gene35341:CDS 70.0%
CCTGCTCGAGGTGGTACGGC+TGG - chr2.3:13395372-13395391 MS.gene35341:intron 70.0%
CGGTGGCAGGGTGGACAAGG+TGG - chr2.3:13394508-13394527 MS.gene35341:CDS 70.0%
GGTCGGTGGCAGGGTGGACA+AGG - chr2.3:13394505-13394524 MS.gene35341:CDS 70.0%
CCCTGCCACCGACCGAGACC+TGG + chr2.3:13394499-13394518 None:intergenic 75.0%
GCCAGGTCTCGGTCGGTGGC+AGG - chr2.3:13394495-13394514 MS.gene35341:CDS 75.0%
GGTCTCGGTCGGTGGCAGGG+TGG - chr2.3:13394499-13394518 MS.gene35341:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 13392783 13395716 13392783 ID=MS.gene35341
chr2.3 mRNA 13392783 13395716 13392783 ID=MS.gene35341.t1;Parent=MS.gene35341
chr2.3 exon 13395691 13395716 13395691 ID=MS.gene35341.t1.exon1;Parent=MS.gene35341.t1
chr2.3 CDS 13395691 13395716 13395691 ID=cds.MS.gene35341.t1;Parent=MS.gene35341.t1
chr2.3 exon 13395388 13395457 13395388 ID=MS.gene35341.t1.exon2;Parent=MS.gene35341.t1
chr2.3 CDS 13395388 13395457 13395388 ID=cds.MS.gene35341.t1;Parent=MS.gene35341.t1
chr2.3 exon 13393428 13394642 13393428 ID=MS.gene35341.t1.exon3;Parent=MS.gene35341.t1
chr2.3 CDS 13393428 13394642 13393428 ID=cds.MS.gene35341.t1;Parent=MS.gene35341.t1
chr2.3 exon 13392783 13393340 13392783 ID=MS.gene35341.t1.exon4;Parent=MS.gene35341.t1
chr2.3 CDS 13392783 13393340 13392783 ID=cds.MS.gene35341.t1;Parent=MS.gene35341.t1
Gene Sequence

>MS.gene35341

ATGGCTCCACCTGGGAAGCTTTCTGGGTTTCATCTTCAAGGTCATCACTGGTATTTTCTTTCTTTCTCTTTTTAACTTCTTTATTATTTATTTATTGGTCTTTTCAGTTGCATTTTCTGTGATTATCAGTTTGAAATTTGTAAGAAAATGACATGTTTTTTAGTGGAATCCAAGTCATACATACATGCAACTTTATAGTTTTAAAATAGATTTTTATGTGATTTTTTTTTCCTGTTTTTTTGGAATTTTGTTTTGGTAGGTTTAGTGATTCTGGGTTGCCAAGTGATATTACCGTGTCAATTGATGGTGTCAATTTCCATCTTCACAAGGTATGCACTTCCATTTCATAATTGTTTCTTTTCTTCCACTAATACTCTATTCATAATAATTTGATAGTGGAGTAGTGGTTGAGATTAGTTTCTTTTGTGGTTCTAGTTTATCCTATTCATTTATGTTCTTTTGTTTTTAGTTTGATCCAATCCAATTAATGTGCCCCTACTTTGTGACCTATTTTACTGTTAGATTATTTTGTTATATCCCTATTGATGTAAAAAAGTTTTATGTTGTCTCCCCTTCACAACCAATCATACGTGTGACTTTATAAGTAGTTATGATTAAAGTTAAATGTTTTTGACTCTTTACATCTGCGGTGTATATGAATTAAATACGTATTTAAATGCAGAGACTATGAACCCTTCATTAACTAGAGATCACACGTAAGTTTCTGTACTTCATTACAGTTATGTTATTGCTAGTTCTTAATTGTCTAGTTAATGATGAAGCCAACATTTTAAATGCATTTAGTTCACTTGATCTACTGTTTTGGAAGTTATGTGGCTACAATTAAAAGGAAAAGGTGAATATTTTGATATATTGGAGAAAATTTATTTGGAAGCTGGTTTTCTCTTTTATTTCAATTTTGAATTCCCGCATAATTGCATGGTGAAATAGGGGGAATTTTTGTGAAACATAATTGCATGGTTTTCTCTTTAATATCTATGTTATGACTTTGATATGATAAATTACTTGTAATGTTGTGTTGGAGTCTGAGTAAGATATGGCGTGAAACTGCAGTTTCCTCTTGTTTCAAGATGTGGAAAAATTGCCCGTACCTGTGAAGATTCCAAGAATACCTATGAGAAGAACCTGACAGTGGTGCTGGAAGAATTTCCTGGGGGTCCTGAAACTTTCTTGATTGTGGCCAAATTCTGTTACGGGTTTCGTGTAGAGCTGACAGCGAAAAATGTAGTATTAGTCCACTGTGCTGCAGAATACCTTGAAATGACGGATGAATACGAAGAAGGCAACTTGTTGACAAAGTCAGAGAGCTTTTTCCATAAAAATATATTGCGCAACTGGAAAGATTGCATTTTGGCTCTCCAAAGCTCTGAGCCTATTCTATCAAGAGCTGAAAATCTTCACTTAGTGGATAAGTGTTTAAACGCTCTATCCATGATGGCTTGCACGGACCCGAGTTTATTTGGATGGCCAATGATGATGTATGGGAGTTTTCAGAGCCCGGGTGGAAGCATTCTTTGGAATGGTATAAATACCGGCGCTAGGATTCGGACCTCAGAATCCGAGTGGTGGTTTGAAGACATCTCATATCTCAGTGTCAGATTGTTTGAGAGCCTTGTTAAGACAATGAGAGCGAGAGGTATGAGACCCGAAAGCCTTGCAGGTGCCATAATGTATTATTCTAGGAAGCACTTGCCAGGTCTCGGTCGGTGGCAGGGTGGACAAGGTGGCAAAACGAGAACAGTTGCCAGTTTTAGTTTGACGCCTGCTAGTGCTACCGTAGATCAGAGGGTTCTTTTGGAAAGCATTGAGAAACTTCTTCCTCAAAAGAAGGGAAAATCTTTTTGCCGTTTCTTACTCGGACTTCTTCGTGTAGCTTCGATATTGAATGTTGGTCAAGCTTGCCAGGAATCTTTGGAGAGAAGAATAGGGATGCAATTGGAACTTGCAACATTAGATGGTCTTCTCATTCCTGCTTATGCAGATTCTGATGCATTGTATAACACAGATTGTATTGAACATATTGTCCATCATTTTGTACTTACGGAATCAAATTTAACCGCCTTTTCTCCTTCATCGCTTGATCCGCAAGCATCATCGTCATCTGAATCGTTAAGGAAAGTTGCAAAGTTGATAGATAGCTATATTGGCGAAATTGCGTCTGATGTTAATTTAAAACCAGAAAAGTTGCGTGCTCTTGCACAAGCCCTTCCCGAGTCATCGAGATCATTGCATGACGGACTCTACCGAGCACTGGACATATATTTCAAGGTTTGATTTCCTGAGCTACATTTTCAATCAATTGCTAATTTATGATCATGAATTGTTAGTTTGATGAATGCTTACGACACTTTGCAGGCTCATCCTTGGCTCTCTGATAACGAGAAAGAAGAAATATGCAACATCATTGATTACCAAAAACTCTCAATCCATGCATGTGCTCATGCATCTCAAAATGATAGGTTACCCCTTAGAGTTGTTCTTCAAGTCTTATTCTTTGAACAGCTGCATTTGAGAACAGCATTAGCCGGATGTGTCAATGCTTTGGATGGCGAAAGCGCTCCAGCAGTTCCTGCTCGAGGTGGTACGGCTGGAGAAATTGTACAAAGAGACGGATGGGTTGCTGTTGTGCGCGAAAATCAGGGTTTGAAGGTGGATATGGATAGAATGAGTACTAGAGTCGGAGAACTTGAAGAAGAATTCAGTAAAATAAAAAAGGAAATGAAAACCGTGACCAAATCTCACAGTGCACGCGGTTCTCCTCGGTTACTTGCAAGAAAACTCGGGTGCAAGCTTGTTCCTAGATTTTCAGATGCTCAACCTGAATCCCTCGATCGTTCTATATCCACACCAAGAGCATCAGTTGAGCGAGCACGCCGTTCCCGTAAGTCCAGACACACAGAAAGTTTTTCTTGA
Protein sequence

>MS.gene35341.t1

MAPPGKLSGFSDSGLPSDITVSIDGVNFHLHKFPLVSRCGKIARTCEDSKNTYEKNLTVVLEEFPGGPETFLIVAKFCYGFRVELTAKNVVLVHCAAEYLEMTDEYEEGNLLTKSESFFHKNILRNWKDCILALQSSEPILSRAENLHLVDKCLNALSMMACTDPSLFGWPMMMYGSFQSPGGSILWNGINTGARIRTSESEWWFEDISYLSVRLFESLVKTMRARGMRPESLAGAIMYYSRKHLPGLGRWQGGQGGKTRTVASFSLTPASATVDQRVLLESIEKLLPQKKGKSFCRFLLGLLRVASILNVGQACQESLERRIGMQLELATLDGLLIPAYADSDALYNTDCIEHIVHHFVLTESNLTAFSPSSLDPQASSSSESLRKVAKLIDSYIGEIASDVNLKPEKLRALAQALPESSRSLHDGLYRALDIYFKAHPWLSDNEKEEICNIIDYQKLSIHACAHASQNDRLPLRVVLQVLFFEQLHLRTALAGCVNALDGESAPAVPARGGTAGEIVQRDGWVAVVRENQGLKVDMDRMSTRVGELEEEFSKIKKEMKTVTKSHSARGSPRLLARKLGCKLVPRFSDAQPESLDRSISTPRASVERARRSRKSRHTESFS