AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90204

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90204.t1 MTR_4g093850 99.253 669 5 0 1 669 1 669 0.0 1384
MS.gene90204.t1 MTR_2g009340 80.495 687 114 9 1 669 1 685 0.0 1101
MS.gene90204.t1 MTR_4g093850 97.200 500 14 0 1 500 1 500 0.0 1013
MS.gene90204.t1 MTR_8g090220 41.878 671 288 12 18 657 27 626 5.23e-173 508
MS.gene90204.t1 MTR_8g090220 45.404 544 222 11 18 543 27 513 3.08e-161 474
MS.gene90204.t1 MTR_2g084595 37.645 603 336 9 27 621 6 576 1.39e-139 422
MS.gene90204.t1 MTR_2g018750 39.675 615 315 13 19 625 1 567 5.89e-136 412
MS.gene90204.t1 MTR_5g015790 38.567 586 306 11 23 599 5 545 2.49e-133 406
MS.gene90204.t1 MTR_8g078900 35.969 645 333 12 34 633 10 619 3.09e-132 404
MS.gene90204.t1 MTR_5g016060 38.014 584 291 9 33 606 29 551 5.37e-129 395
MS.gene90204.t1 MTR_5g032780 40.476 546 275 10 23 545 5 523 1.09e-128 394
MS.gene90204.t1 MTR_1g094850 38.019 626 328 14 19 635 1 575 1.63e-128 393
MS.gene90204.t1 MTR_4g022330 36.557 610 334 9 33 639 24 583 3.74e-126 387
MS.gene90204.t1 MTR_8g078900 36.276 623 317 12 56 633 2 589 7.02e-125 384
MS.gene90204.t1 MTR_1g082690 35.884 588 285 10 29 610 43 544 1.49e-121 373
MS.gene90204.t1 MTR_1g014070 39.764 508 258 8 39 540 22 487 2.04e-121 375
MS.gene90204.t1 MTR_8g069330 40.809 544 241 11 23 545 5 488 1.76e-120 372
MS.gene90204.t1 MTR_1g064360 37.782 532 275 7 23 548 5 486 2.95e-120 370
MS.gene90204.t1 MTR_1g106430 37.316 544 274 9 23 550 5 497 4.23e-120 372
MS.gene90204.t1 MTR_8g078900 37.522 573 298 11 86 633 42 579 9.47e-120 370
MS.gene90204.t1 MTR_2g048390 41.754 479 245 7 19 490 1 452 4.50e-118 361
MS.gene90204.t1 MTR_4g095430 36.177 586 312 9 33 615 2 528 1.52e-116 361
MS.gene90204.t1 MTR_1g088885 33.180 651 356 13 38 669 31 621 2.46e-112 351
MS.gene90204.t1 MTR_4g134210 37.970 532 276 11 23 552 5 484 4.48e-112 350
MS.gene90204.t1 MTR_7g073980 35.233 579 307 13 40 608 29 549 4.73e-111 348
MS.gene90204.t1 MTR_2g084595 38.004 471 256 7 27 491 6 446 1.23e-106 331
MS.gene90204.t1 MTR_2g027410 36.585 533 274 8 23 552 5 476 1.32e-106 335
MS.gene90204.t1 MTR_7g108290 33.441 622 343 13 33 636 26 594 5.38e-106 336
MS.gene90204.t1 MTR_3g062540 33.007 612 323 12 33 640 13 541 5.08e-99 315
MS.gene90204.t1 MTR_5g075640 31.884 621 332 13 33 643 14 553 1.08e-97 312
MS.gene90204.t1 MTR_8g078900 36.961 487 244 9 34 490 10 463 3.82e-97 306
MS.gene90204.t1 MTR_2g027410 37.849 465 225 8 91 552 1 404 1.22e-92 296
MS.gene90204.t1 MTR_5g016060 36.016 497 247 9 120 606 2 437 5.63e-92 295
MS.gene90204.t1 MTR_3g062540 32.530 581 305 12 64 640 2 499 1.86e-87 283
MS.gene90204.t1 MTR_5g075640 31.017 590 316 13 64 643 2 510 8.34e-86 279
MS.gene90204.t1 MTR_8g078900 39.277 415 209 8 86 490 42 423 6.46e-85 273
MS.gene90204.t1 MTR_4g011530 36.407 423 206 9 207 623 26 391 6.27e-79 258
MS.gene90204.t1 MTR_2g102730 35.684 468 233 10 216 669 10 423 1.63e-78 256
MS.gene90204.t1 MTR_1g035720 29.245 530 260 15 47 539 6 457 7.35e-52 188
MS.gene90204.t1 MTR_4g074190 27.768 587 324 16 46 606 5 517 8.34e-52 188
MS.gene90204.t1 MTR_4g074190 27.440 543 298 12 86 606 3 471 2.83e-49 180
MS.gene90204.t1 MTR_4g045767 27.018 607 330 18 47 623 7 530 9.77e-49 179
MS.gene90204.t1 MTR_4g074190 26.850 473 264 13 46 494 5 419 7.88e-38 146
MS.gene90204.t1 MTR_1g105870 35.931 231 117 4 51 279 38 239 7.60e-35 133
MS.gene90204.t1 MTR_2g010890 24.945 457 270 7 110 559 1 391 1.74e-31 128
MS.gene90204.t1 MTR_2g048375 37.879 132 72 2 507 635 1 125 5.65e-21 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90204.t1 AT5G64330 70.139 720 144 12 1 649 2 721 0.0 960
MS.gene90204.t1 AT5G64330 70.279 646 124 11 1 578 2 647 0.0 860
MS.gene90204.t1 AT5G64330 71.012 583 108 9 57 578 6 588 0.0 785
MS.gene90204.t1 AT5G10250 40.063 634 273 13 30 637 36 588 1.03e-151 453
MS.gene90204.t1 AT1G30440 38.779 655 356 11 19 654 1 629 9.73e-150 449
MS.gene90204.t1 AT5G10250 40.128 623 273 13 30 637 36 573 4.70e-148 443
MS.gene90204.t1 AT5G03250 41.682 535 267 8 25 547 7 508 2.51e-135 410
MS.gene90204.t1 AT3G44820 36.153 603 349 7 27 618 6 583 9.28e-130 397
MS.gene90204.t1 AT5G13600 39.286 616 302 17 19 623 1 555 1.12e-129 395
MS.gene90204.t1 AT2G14820 38.447 541 272 9 23 550 5 497 4.85e-125 385
MS.gene90204.t1 AT2G14820 38.447 541 272 9 23 550 5 497 4.85e-125 385
MS.gene90204.t1 AT5G67440 39.810 525 257 8 23 541 5 476 2.10e-123 379
MS.gene90204.t1 AT5G67440 39.810 525 257 8 23 541 5 476 2.10e-123 379
MS.gene90204.t1 AT5G67385 39.731 521 260 8 33 550 14 483 2.05e-122 377
MS.gene90204.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene90204.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene90204.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene90204.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene90204.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene90204.t1 AT1G67900 39.674 552 267 8 23 548 5 516 5.11e-121 374
MS.gene90204.t1 AT5G66560 33.728 676 337 13 34 640 9 642 1.00e-115 362
MS.gene90204.t1 AT4G37590 37.879 594 269 15 23 599 5 515 1.99e-115 358
MS.gene90204.t1 AT4G31820 37.729 546 269 13 23 562 5 485 8.84e-115 356
MS.gene90204.t1 AT5G67440 40.164 488 234 7 59 541 5 439 1.04e-114 355
MS.gene90204.t1 AT5G48800 35.489 603 330 7 37 636 34 580 1.29e-114 357
MS.gene90204.t1 AT1G03010 33.385 644 348 14 23 641 16 603 1.70e-112 352
MS.gene90204.t1 AT3G08570 35.197 608 326 11 40 640 30 576 4.02e-112 350
MS.gene90204.t1 AT1G03010 33.760 625 334 13 42 641 7 576 2.68e-111 348
MS.gene90204.t1 AT1G03010 33.760 625 334 13 42 641 33 602 7.54e-111 348
MS.gene90204.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene90204.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene90204.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene90204.t1 AT5G67385 39.059 489 244 8 65 550 1 438 1.58e-108 339
MS.gene90204.t1 AT2G30520 34.975 589 293 13 34 615 20 525 2.40e-108 340
MS.gene90204.t1 AT5G66560 34.098 654 320 13 56 640 2 613 3.21e-108 341
MS.gene90204.t1 AT2G47860 34.500 600 315 10 38 618 32 572 9.67e-108 340
MS.gene90204.t1 AT5G47800 36.902 523 271 9 23 539 5 474 1.57e-107 337
MS.gene90204.t1 AT5G47800 36.902 523 271 9 23 539 5 474 1.57e-107 337
MS.gene90204.t1 AT5G47800 36.902 523 271 9 23 539 5 474 1.57e-107 337
MS.gene90204.t1 AT3G26490 36.823 554 268 12 25 545 7 511 2.20e-107 337
MS.gene90204.t1 AT3G26490 36.823 554 268 12 25 545 7 511 2.20e-107 337
MS.gene90204.t1 AT3G26490 36.823 554 268 12 25 545 7 511 2.20e-107 337
MS.gene90204.t1 AT3G08660 35.043 585 287 13 40 615 30 530 1.39e-106 335
MS.gene90204.t1 AT5G47800 36.902 523 271 9 23 539 118 587 7.62e-106 336
MS.gene90204.t1 AT5G66560 36.034 580 304 11 86 640 66 603 9.49e-105 332
MS.gene90204.t1 AT2G47860 33.226 623 315 11 38 618 32 595 1.22e-102 327
MS.gene90204.t1 AT4G31820 37.223 497 242 12 71 562 1 432 3.32e-99 314
MS.gene90204.t1 AT2G23050 35.000 540 255 12 23 553 5 457 2.81e-97 308
MS.gene90204.t1 AT2G30520 34.168 559 278 13 64 615 2 477 1.42e-95 305
MS.gene90204.t1 AT2G30520 34.168 559 278 13 64 615 2 477 1.42e-95 305
MS.gene90204.t1 AT3G08660 34.477 554 270 13 71 615 1 470 1.60e-95 304
MS.gene90204.t1 AT3G50840 33.114 607 321 13 38 633 10 542 9.72e-95 304
MS.gene90204.t1 AT5G03250 39.627 429 216 7 129 547 1 396 3.84e-92 294
MS.gene90204.t1 AT3G49970 33.694 555 258 11 56 608 6 452 7.44e-88 284
MS.gene90204.t1 AT3G49970 33.694 555 258 11 56 608 6 452 7.44e-88 284
MS.gene90204.t1 AT3G50840 32.815 579 306 12 65 633 1 506 9.23e-83 271
MS.gene90204.t1 AT3G50840 32.815 579 306 12 65 633 1 506 9.23e-83 271
MS.gene90204.t1 AT3G26490 34.600 500 245 12 25 491 7 457 2.11e-82 269
MS.gene90204.t1 AT5G66560 33.852 514 242 11 34 491 9 480 2.51e-82 268
MS.gene90204.t1 AT2G47860 34.783 483 214 9 38 490 32 443 4.53e-80 261
MS.gene90204.t1 AT1G52770 36.183 503 232 16 205 669 3 454 6.04e-80 261
MS.gene90204.t1 AT3G08660 33.978 465 230 9 40 498 30 423 2.87e-79 259
MS.gene90204.t1 AT3G15570 34.047 467 238 9 230 669 29 452 2.74e-76 251
MS.gene90204.t1 AT2G47860 33.535 495 229 10 38 491 32 467 4.02e-75 249
MS.gene90204.t1 AT2G47860 31.041 509 277 8 129 618 1 454 7.12e-69 234
MS.gene90204.t1 AT2G47860 31.041 509 277 8 129 618 1 454 7.12e-69 234
MS.gene90204.t1 AT2G23050 33.879 428 193 10 129 553 1 341 6.56e-65 219
MS.gene90204.t1 AT3G19850 28.355 529 280 9 44 540 3 464 2.96e-56 201
MS.gene90204.t1 AT1G50280 28.487 509 292 13 46 535 5 460 8.81e-49 179
MS.gene90204.t1 AT1G50280 29.374 463 261 11 86 535 18 427 1.00e-47 176
MS.gene90204.t1 AT5G17580 28.161 522 298 12 40 553 1 453 9.66e-44 166
MS.gene90204.t1 AT5G17580 27.969 522 299 12 40 553 16 468 1.16e-43 166
MS.gene90204.t1 AT3G22104 26.477 491 275 13 46 526 6 420 4.37e-39 151
MS.gene90204.t1 AT3G03510 26.799 556 278 17 94 642 4 437 8.01e-38 147
MS.gene90204.t1 AT5G48130 32.911 237 145 4 37 269 32 258 3.89e-34 138
MS.gene90204.t1 AT5G48130 30.049 203 113 4 436 614 364 561 2.27e-19 92.8
MS.gene90204.t1 AT3G22104 24.821 419 232 10 115 526 3 345 1.08e-25 110
MS.gene90204.t1 AT3G22104 24.821 419 232 10 115 526 3 345 1.08e-25 110
MS.gene90204.t1 AT3G22104 24.821 419 232 10 115 526 3 345 1.08e-25 110
MS.gene90204.t1 AT3G49900 36.250 160 84 5 34 184 55 205 6.34e-14 75.5
MS.gene90204.t1 AT3G49900 33.981 103 63 3 445 546 214 312 4.34e-11 66.2
MS.gene90204.t1 AT3G49900 36.250 160 84 5 34 184 55 205 6.39e-14 75.5

Find 138 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCATTTCGTTCCATTT+TGG 0.129719 4.2:-19743399 None:intergenic
CTGTTCGGTTCTTCAGAAAT+TGG 0.136199 4.2:-19745499 None:intergenic
GGACTTCATATGATTGTTTC+TGG 0.160796 4.2:+19743690 MS.gene90204:CDS
CCACTCTTGCCTTGGCATTC+AGG 0.181350 4.2:-19744170 None:intergenic
CCTTTCATCGACAAAGTTTC+AGG 0.183368 4.2:+19744239 MS.gene90204:CDS
ACAGCAGCTGGAAGGGAATA+TGG 0.207369 4.2:+19742240 MS.gene90204:CDS
TAGAGTCATTACTGCTATTA+AGG 0.220033 4.2:+19743503 MS.gene90204:CDS
GGAAGCTGGCTTCTCCAATT+TGG 0.236985 4.2:-19742503 None:intergenic
AAAATGAACGACTGCCATTA+AGG 0.241454 4.2:+19745113 MS.gene90204:CDS
TACGCCCACCTTATTCCTTT+TGG 0.245910 4.2:-19743348 None:intergenic
TAGCTGTTGTCCATCCTTCT+TGG 0.246421 4.2:-19745279 None:intergenic
ACTTGCACTACTACCCTTAA+TGG 0.258617 4.2:-19745127 None:intergenic
TTTGGAGCTTCAACATGATA+TGG 0.264232 4.2:+19745354 MS.gene90204:CDS
GAGTGTGAAGACAAAATATT+TGG 0.272726 4.2:+19745336 MS.gene90204:CDS
ATTACTGCTATTAAGGTAAA+AGG 0.292860 4.2:+19743510 MS.gene90204:CDS
TAATGCATTATGCAACTAAA+TGG 0.300901 4.2:+19743562 MS.gene90204:CDS
AGCAACTCCATAGCAGAATT+TGG 0.307927 4.2:-19743079 None:intergenic
TTGACAGCAAGCAACATTTC+CGG 0.311281 4.2:+19743108 MS.gene90204:CDS
TGAAGCTTTCGTCGCTCTTC+CGG 0.314769 4.2:-19743373 None:intergenic
TTCAATCTTAACACCATGTT+TGG 0.322863 4.2:-19742283 None:intergenic
GAGAATGGCAAACATGTTAA+AGG 0.326315 4.2:+19743833 MS.gene90204:CDS
GCTGCAAGTTCAAATGCTTC+AGG 0.329025 4.2:-19743057 None:intergenic
CTAGCCAACAGCTCTCTAAA+AGG 0.341374 4.2:+19745184 MS.gene90204:CDS
TGTTGCTTGTTTCCTCTCCT+TGG 0.344019 4.2:-19743617 None:intergenic
CGAAAGCTTCAAGCCCAAAA+TGG 0.344305 4.2:+19743385 MS.gene90204:CDS
AGTGATCTTCAAGTCCAAAT+TGG 0.350920 4.2:+19742489 MS.gene90204:CDS
CCTTCTCAGGCTTCTGAGAA+TGG 0.376751 4.2:+19743818 MS.gene90204:CDS
GTGCTAATCCAAAAGGAATA+AGG 0.382939 4.2:+19743340 MS.gene90204:CDS
AACAGGAAAACACTTCTAGA+AGG 0.384225 4.2:+19745241 MS.gene90204:CDS
CAGCAGCTGGAAGGGAATAT+GGG 0.385850 4.2:+19742241 MS.gene90204:CDS
GATACTCAGCAGCACATCTT+AGG 0.386146 4.2:-19743131 None:intergenic
TCTCGTGTAGCTTCCTTCTC+AGG 0.398409 4.2:+19743805 MS.gene90204:CDS
TTGAAGATGTTTCAATCCTT+AGG 0.401499 4.2:+19743466 MS.gene90204:CDS
TCAGAAAAGGATTGTCTACT+TGG 0.415289 4.2:-19744126 None:intergenic
CTTCTAGTTGTCTTTCTGTT+CGG 0.415753 4.2:-19745514 None:intergenic
AAATGTGGAAAATCATGATA+TGG 0.424301 4.2:+19745474 MS.gene90204:CDS
CTTTCATGCTCAGTTAGTGT+TGG 0.426046 4.2:-19745025 None:intergenic
TCCTGTTTGTTACCATTGGT+TGG 0.426066 4.2:-19745225 None:intergenic
GTGTCGTTCCCCGCCAGTCC+TGG 0.426869 4.2:-19743588 None:intergenic
CTATAGACTCGCTGCATCTT+CGG 0.430254 4.2:-19743305 None:intergenic
AACTAAATGGCTACCAGGAC+TGG 0.435309 4.2:+19743575 MS.gene90204:CDS
ATAGCTATCTTATTCAAGTC+GGG 0.438601 4.2:-19743015 None:intergenic
AAGCCTTGCCACTCTTGCCT+TGG 0.438871 4.2:-19744178 None:intergenic
AAACATGGTGTTAAGATTGA+AGG 0.441638 4.2:+19742285 MS.gene90204:CDS
TCAAATGCTTCAGGCCCTCC+AGG 0.443506 4.2:-19743048 None:intergenic
AAATGAACGACTGCCATTAA+GGG 0.444360 4.2:+19745114 MS.gene90204:CDS
AGCTAGTGCCTCCTGATTGG+TGG 0.449333 4.2:+19743442 MS.gene90204:CDS
CCTGAAACTTTGTCGATGAA+AGG 0.449456 4.2:-19744239 None:intergenic
TGCGTGCATGCAGGCGTCAA+TGG 0.453590 4.2:-19745086 None:intergenic
GGCACTAGCTGATTCCTGCT+CGG 0.457366 4.2:-19743429 None:intergenic
ATCAGCTAGTGCCTCCTGAT+TGG 0.466020 4.2:+19743439 MS.gene90204:CDS
CACATGCTTGTGATCAGAAA+AGG 0.471840 4.2:-19744139 None:intergenic
ACCAACCAATGGTAACAAAC+AGG 0.475079 4.2:+19745224 MS.gene90204:CDS
TGATTACTGATTTAGAGAAA+AGG 0.477093 4.2:+19743962 MS.gene90204:CDS
TGCAGAAGCTTTACCTGAGT+CGG 0.477481 4.2:+19744266 MS.gene90204:CDS
GATTACTGATTTAGAGAAAA+GGG 0.477757 4.2:+19743963 MS.gene90204:CDS
ACATCTTCAAACCACCAATC+AGG 0.482701 4.2:-19743453 None:intergenic
CTTAACACCATGTTTGGTTG+AGG 0.483711 4.2:-19742277 None:intergenic
AGATTTGAACTGATTGGTGC+TGG 0.486553 4.2:+19743537 MS.gene90204:CDS
TACCTTGTGCAAGTGGAAGC+TGG 0.487213 4.2:-19742517 None:intergenic
AAATGGCTACCAGGACTGGC+GGG 0.494907 4.2:+19743579 MS.gene90204:CDS
AAAGCTTGTGCTAATCCAAA+AGG 0.497825 4.2:+19743333 MS.gene90204:CDS
ATAGCTATGGATGATCTCCC+TGG 0.498197 4.2:+19743030 MS.gene90204:CDS
ATTTGGACTTGAAGATCACT+TGG 0.499439 4.2:-19742486 None:intergenic
CGGGGTCGCGAGAATCATAT+AGG 0.499847 4.2:-19742996 None:intergenic
GCACTAGCTGATTCCTGCTC+GGG 0.500897 4.2:-19743428 None:intergenic
GAAGCGTCTCTGTCGTGTAA+TGG 0.510244 4.2:+19745048 MS.gene90204:CDS
GAAAAGATACCTTGTGCAAG+TGG 0.511668 4.2:-19742524 None:intergenic
CAACACTAACTGAGCATGAA+AGG 0.512884 4.2:+19745026 MS.gene90204:CDS
GGATCTTGTTCAGAGGTTGT+TGG 0.513765 4.2:+19744065 MS.gene90204:CDS
GGAATAAGGTGGGCGTATAC+CGG 0.514993 4.2:+19743354 MS.gene90204:CDS
GGCAAGAGTGGCAAGGCTTG+TGG 0.518056 4.2:+19744182 MS.gene90204:CDS
GCGGTGACAACAACAGTAGC+TGG 0.519169 4.2:+19743661 MS.gene90204:CDS
CATAGCTATCTTATTCAAGT+CGG 0.522649 4.2:-19743016 None:intergenic
ACTGATTTAGAGAAAAGGGT+TGG 0.526187 4.2:+19743967 MS.gene90204:CDS
AAGTGAAACAGCAGCTGGAA+GGG 0.532024 4.2:+19742233 MS.gene90204:CDS
GGTATGAGATTTGAACTGAT+TGG 0.534123 4.2:+19743531 MS.gene90204:CDS
CGACTTGAATAAGATAGCTA+TGG 0.536962 4.2:+19743017 MS.gene90204:CDS
AGCAAAAGCACACATCAGCT+TGG 0.538253 4.2:+19745410 MS.gene90204:CDS
CCTGAATGCCAAGGCAAGAG+TGG 0.539436 4.2:+19744170 MS.gene90204:CDS
TAACAAAGTGATCAATCCTA+AGG 0.539725 4.2:-19743482 None:intergenic
ATGACTGAGGATCTAGAAGA+AGG 0.540350 4.2:+19743159 MS.gene90204:CDS
CGCAATCTTTCCAAGAAGGA+TGG 0.541041 4.2:+19745269 MS.gene90204:CDS
CGAACAGAAAGACAACTAGA+AGG 0.543947 4.2:+19745515 MS.gene90204:CDS
CACACATCAGCTTGGACTAG+TGG 0.544231 4.2:+19745418 MS.gene90204:CDS
TGAAAGACTCAAGCCCGAGC+AGG 0.544479 4.2:+19743415 MS.gene90204:CDS
CTCTGAAAAGCTGTCACCGT+GGG 0.544588 4.2:+19743263 MS.gene90204:CDS
CTTGCAGCCAAATTCTGCTA+TGG 0.546691 4.2:+19743072 MS.gene90204:CDS
CTTGTTCAAGAACAAACTGA+AGG 0.548683 4.2:+19744096 MS.gene90204:CDS
TCAAACCAAGGATCAAAAGA+TGG 0.555075 4.2:+19743737 MS.gene90204:CDS
TCAGAAAGTGAAACAGCAGC+TGG 0.555263 4.2:+19742228 MS.gene90204:CDS
TATCCTTTGCTATCTAGAAG+TGG 0.555707 4.2:+19742958 MS.gene90204:CDS
CTCAGCAGCACATCTTAGGC+CGG 0.556722 4.2:-19743127 None:intergenic
CCATTCTCAGAAGCCTGAGA+AGG 0.562143 4.2:-19743818 None:intergenic
TTCATCAAAGAGGCAACTCA+TGG 0.562485 4.2:+19742310 MS.gene90204:CDS
TCAGAAACTCTAGCCGACTC+AGG 0.569094 4.2:-19744279 None:intergenic
AGCAATTGATTCATATCTCA+AGG 0.570287 4.2:+19744317 MS.gene90204:CDS
AAAGTGAAACAGCAGCTGGA+AGG 0.573228 4.2:+19742232 MS.gene90204:CDS
AGCAGCTGGAAGGGAATATG+GGG 0.577271 4.2:+19742242 MS.gene90204:CDS
ACTTACTAAAATGACAAATG+TGG 0.586018 4.2:+19745459 MS.gene90204:CDS
CATCCTTCTTGGAAAGATTG+CGG 0.587019 4.2:-19745268 None:intergenic
ACACATCAGCTTGGACTAGT+GGG 0.588970 4.2:+19745419 MS.gene90204:CDS
GATGCAGCGAGTCTATAGCT+TGG 0.589137 4.2:+19743310 MS.gene90204:CDS
ACAGGAAAACACTTCTAGAA+GGG 0.589157 4.2:+19745242 MS.gene90204:CDS
AACAACAGTAGCTGGAGAGG+TGG 0.595238 4.2:+19743669 MS.gene90204:CDS
TAAATGGCTACCAGGACTGG+CGG 0.596021 4.2:+19743578 MS.gene90204:CDS
GTTCTCACCTCAACCAAACA+TGG 0.597650 4.2:+19742270 MS.gene90204:CDS
CTATGGATGATCTCCCTGGA+GGG 0.599558 4.2:+19743034 MS.gene90204:CDS
TTTCCACTTCTAGATAGCAA+AGG 0.603021 4.2:-19742961 None:intergenic
AATGGCTACCAGGACTGGCG+GGG 0.608558 4.2:+19743580 MS.gene90204:CDS
CAAATGCTTCAGGCCCTCCA+GGG 0.613888 4.2:-19743047 None:intergenic
ACACCGCAATCTTTCCAAGA+AGG 0.615108 4.2:+19745265 MS.gene90204:CDS
GGGAACGACACTACAATCCA+AGG 0.616043 4.2:+19743600 MS.gene90204:CDS
AGCCAGCTTCCACTTGCACA+AGG 0.623189 4.2:+19742515 MS.gene90204:CDS
ATGCCAAGGCAAGAGTGGCA+AGG 0.625949 4.2:+19744175 MS.gene90204:CDS
TCTGATCACAAGCATGTGGG+AGG 0.626741 4.2:+19744144 MS.gene90204:CDS
CTAATCCAAAAGGAATAAGG+TGG 0.630264 4.2:+19743343 MS.gene90204:CDS
GACAACAACAGTAGCTGGAG+AGG 0.638998 4.2:+19743666 MS.gene90204:CDS
TAGCTATCTTATTCAAGTCG+GGG 0.642768 4.2:-19743014 None:intergenic
ACAGAAAGACAACTAGAAGG+TGG 0.644997 4.2:+19745518 MS.gene90204:CDS
TGAATCTCAGTACCAACCAA+TGG 0.650255 4.2:+19745213 MS.gene90204:CDS
AACTTCAAGTCTTCAAACCA+AGG 0.650442 4.2:+19743725 MS.gene90204:CDS
TATGCAACTAAATGGCTACC+AGG 0.651310 4.2:+19743570 MS.gene90204:CDS
AAGTGAAACTATGTATGATG+TGG 0.652037 4.2:+19744044 MS.gene90204:CDS
CGACACTACAATCCAAGGAG+AGG 0.656624 4.2:+19743605 MS.gene90204:CDS
TCGGCTAGAGTTTCTGACGA+TGG 0.658303 4.2:+19744285 MS.gene90204:CDS
GGGAGGTAACCTGAATGCCA+AGG 0.660203 4.2:+19744161 MS.gene90204:CDS
TGTGGACAGTTATCTTACAG+AGG 0.662209 4.2:+19744200 MS.gene90204:CDS
GAGACACTGTCTTTCTGCGG+CGG 0.666070 4.2:-19743786 None:intergenic
CATCAGCTTGGACTAGTGGG+TGG 0.666857 4.2:+19745422 MS.gene90204:CDS
TAATCCAAAAGGAATAAGGT+GGG 0.670549 4.2:+19743344 MS.gene90204:CDS
TCTTCAAACCACCAATCAGG+AGG 0.670951 4.2:-19743450 None:intergenic
CACGAGACACTGTCTTTCTG+CGG 0.688596 4.2:-19743789 None:intergenic
GCTCTGAAAAGCTGTCACCG+TGG 0.705207 4.2:+19743262 MS.gene90204:CDS
ATGATGTGGATCTTGTTCAG+AGG 0.708047 4.2:+19744058 MS.gene90204:CDS
GCTATGGATGATCTCCCTGG+AGG 0.717645 4.2:+19743033 MS.gene90204:CDS
TGAGTATCTTGAAATGACTG+AGG 0.729252 4.2:+19743146 MS.gene90204:CDS
GCAGCTGGAAGGGAATATGG+GGG 0.752997 4.2:+19742243 MS.gene90204:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATAACTTTCT+AGG - chr4.2:19742581-19742600 None:intergenic 10.0%
!! ATATGACAATATACTTATTT+AGG + chr4.2:19744552-19744571 MS.gene90204:intron 15.0%
!! TTCAATTAACTTACATAAAA+TGG - chr4.2:19742424-19742443 None:intergenic 15.0%
!!! GTTATTTTATTTTTTCTCTA+AGG + chr4.2:19742586-19742605 MS.gene90204:intron 15.0%
!!! TTTTTATTTTCAATTACTCT+AGG + chr4.2:19744395-19744414 MS.gene90204:intron 15.0%
!! AATTAGTTAGACATTGTTTA+AGG - chr4.2:19743901-19743920 None:intergenic 20.0%
!! CTAATATTTCAAGATAACAT+TGG + chr4.2:19744616-19744635 MS.gene90204:intron 20.0%
!!! TAGTTTTTTTCTTAACCTTT+CGG - chr4.2:19742865-19742884 None:intergenic 20.0%
! AAAGATATAATTGCGATGAA+GGG - chr4.2:19744659-19744678 None:intergenic 25.0%
! AAATGTGGAAAATCATGATA+TGG + chr4.2:19745474-19745493 MS.gene90204:CDS 25.0%
! AATATCAAACTATCTCTAAC+AGG + chr4.2:19743927-19743946 MS.gene90204:intron 25.0%
! ACATAAGACATCAATGAAAT+TGG - chr4.2:19744890-19744909 None:intergenic 25.0%
! ACTTACTAAAATGACAAATG+TGG + chr4.2:19745459-19745478 MS.gene90204:CDS 25.0%
! ATATTTCAAGATAACATTGG+AGG + chr4.2:19744619-19744638 MS.gene90204:intron 25.0%
! ATTACTGCTATTAAGGTAAA+AGG + chr4.2:19743510-19743529 MS.gene90204:CDS 25.0%
! CAATTAAGGATGATTCATTT+GGG + chr4.2:19742742-19742761 MS.gene90204:intron 25.0%
! CTCATTGAAAATGAAATTTG+AGG - chr4.2:19744700-19744719 None:intergenic 25.0%
! CTTATAATCTATAAACCGAA+AGG + chr4.2:19742847-19742866 MS.gene90204:intron 25.0%
! CTTTCGGTTTATAGATTATA+AGG - chr4.2:19742849-19742868 None:intergenic 25.0%
! TAAAGATATAATTGCGATGA+AGG - chr4.2:19744660-19744679 None:intergenic 25.0%
! TAATGCATTATGCAACTAAA+TGG + chr4.2:19743562-19743581 MS.gene90204:CDS 25.0%
! TCAATTAAGGATGATTCATT+TGG + chr4.2:19742741-19742760 MS.gene90204:intron 25.0%
! TCTTAAAAGCAAAGTTGTAT+TGG + chr4.2:19744483-19744502 MS.gene90204:intron 25.0%
!!! AATCACTTTTCAAAGGATAA+AGG + chr4.2:19744345-19744364 MS.gene90204:intron 25.0%
!!! AGTAATAGTAGTTTTAGTAG+CGG + chr4.2:19743642-19743661 MS.gene90204:CDS 25.0%
!!! ATCACTTTTCAAAGGATAAA+GGG + chr4.2:19744346-19744365 MS.gene90204:intron 25.0%
!!! GATTACTGATTTAGAGAAAA+GGG + chr4.2:19743963-19743982 MS.gene90204:CDS 25.0%
!!! GGTAATTAATCACTTTTCAA+AGG + chr4.2:19744338-19744357 MS.gene90204:intron 25.0%
!!! TGATTACTGATTTAGAGAAA+AGG + chr4.2:19743962-19743981 MS.gene90204:CDS 25.0%
AAACTCATTAACAACTTGAG+CGG - chr4.2:19742650-19742669 None:intergenic 30.0%
AAGTGAAACTATGTATGATG+TGG + chr4.2:19744044-19744063 MS.gene90204:CDS 30.0%
ACCAAACATACACTCAATTA+AGG + chr4.2:19742728-19742747 MS.gene90204:intron 30.0%
AGATTCACATGATATGATCA+TGG + chr4.2:19744951-19744970 MS.gene90204:intron 30.0%
AGGATACTTTCACAACAAAA+AGG - chr4.2:19742944-19742963 None:intergenic 30.0%
AGGTAGTTACAAGTTAGTTA+TGG - chr4.2:19743881-19743900 None:intergenic 30.0%
ATAGCTATCTTATTCAAGTC+GGG - chr4.2:19743018-19743037 None:intergenic 30.0%
CATAGCTATCTTATTCAAGT+CGG - chr4.2:19743019-19743038 None:intergenic 30.0%
GAGTGTGAAGACAAAATATT+TGG + chr4.2:19745336-19745355 MS.gene90204:CDS 30.0%
GATCTGTGAAACAAATTAAC+AGG + chr4.2:19744997-19745016 MS.gene90204:intron 30.0%
GTAAAATTTGAGTCAGAACT+TGG + chr4.2:19744433-19744452 MS.gene90204:intron 30.0%
GTTTATAGATTATAAGGACC+TGG - chr4.2:19742843-19742862 None:intergenic 30.0%
TAACAAAGTGATCAATCCTA+AGG - chr4.2:19743485-19743504 None:intergenic 30.0%
TAATCCAAAAGGAATAAGGT+GGG + chr4.2:19743344-19743363 MS.gene90204:CDS 30.0%
TAATGAGTTTCAGTGATGTT+TGG + chr4.2:19742660-19742679 MS.gene90204:intron 30.0%
TAGAGTCATTACTGCTATTA+AGG + chr4.2:19743503-19743522 MS.gene90204:CDS 30.0%
TCCTTAATTGAGTGTATGTT+TGG - chr4.2:19742732-19742751 None:intergenic 30.0%
TTCAATCTTAACACCATGTT+TGG - chr4.2:19742286-19742305 None:intergenic 30.0%
TTGAAGATGTTTCAATCCTT+AGG + chr4.2:19743466-19743485 MS.gene90204:CDS 30.0%
! AAACATGGTGTTAAGATTGA+AGG + chr4.2:19742285-19742304 MS.gene90204:CDS 30.0%
! AATAACCATCTTTTGATCCT+TGG - chr4.2:19743745-19743764 None:intergenic 30.0%
! AGCAATTGATTCATATCTCA+AGG + chr4.2:19744317-19744336 MS.gene90204:CDS 30.0%
! ATTGAAGGTTTTCATCAAAG+AGG + chr4.2:19742300-19742319 MS.gene90204:CDS 30.0%
! CTTTCATTTCGTTCCATTTT+GGG - chr4.2:19743401-19743420 None:intergenic 30.0%
! TCTTTCATTTCGTTCCATTT+TGG - chr4.2:19743402-19743421 None:intergenic 30.0%
!! CTCATTTTAGAAGAATGAGT+CGG + chr4.2:19744911-19744930 MS.gene90204:intron 30.0%
!! TCACTTTTCAAAGGATAAAG+GGG + chr4.2:19744347-19744366 MS.gene90204:intron 30.0%
!!! AAGCAAAGTTGTATTGGTTT+TGG + chr4.2:19744489-19744508 MS.gene90204:intron 30.0%
AAAATGAACGACTGCCATTA+AGG + chr4.2:19745113-19745132 MS.gene90204:CDS 35.0%
AAAGCTTGTGCTAATCCAAA+AGG + chr4.2:19743333-19743352 MS.gene90204:CDS 35.0%
AAATGAACGACTGCCATTAA+GGG + chr4.2:19745114-19745133 MS.gene90204:CDS 35.0%
AACAGGAAAACACTTCTAGA+AGG + chr4.2:19745241-19745260 MS.gene90204:CDS 35.0%
AACTTCAAGTCTTCAAACCA+AGG + chr4.2:19743725-19743744 MS.gene90204:CDS 35.0%
AGTGATCTTCAAGTCCAAAT+TGG + chr4.2:19742489-19742508 MS.gene90204:CDS 35.0%
AGTGATGTTTGGTATCAAGT+TGG + chr4.2:19742671-19742690 MS.gene90204:intron 35.0%
ATGCAGCTTCTAGTATATAG+TGG + chr4.2:19744765-19744784 MS.gene90204:intron 35.0%
ATGGCAAACATGTTAAAGGT+AGG + chr4.2:19743837-19743856 MS.gene90204:intron 35.0%
ATTTGGACTTGAAGATCACT+TGG - chr4.2:19742489-19742508 None:intergenic 35.0%
CGACTTGAATAAGATAGCTA+TGG + chr4.2:19743017-19743036 MS.gene90204:CDS 35.0%
CTAATCCAAAAGGAATAAGG+TGG + chr4.2:19743343-19743362 MS.gene90204:CDS 35.0%
CTTGTTCAAGAACAAACTGA+AGG + chr4.2:19744096-19744115 MS.gene90204:CDS 35.0%
GAACAACTTGGTTTATAGGT+TGG - chr4.2:19742628-19742647 None:intergenic 35.0%
GAGAATGGCAAACATGTTAA+AGG + chr4.2:19743833-19743852 MS.gene90204:CDS 35.0%
GCTTCTAGTATATAGTGGTA+TGG + chr4.2:19744770-19744789 MS.gene90204:intron 35.0%
GGACTTCATATGATTGTTTC+TGG + chr4.2:19743690-19743709 MS.gene90204:CDS 35.0%
GGTATGAGATTTGAACTGAT+TGG + chr4.2:19743531-19743550 MS.gene90204:CDS 35.0%
GTGCTAATCCAAAAGGAATA+AGG + chr4.2:19743340-19743359 MS.gene90204:CDS 35.0%
TAGCTATCTTATTCAAGTCG+GGG - chr4.2:19743017-19743036 None:intergenic 35.0%
TATACTAGAAGCTGCATTCA+CGG - chr4.2:19744762-19744781 None:intergenic 35.0%
TCAAACCAAGGATCAAAAGA+TGG + chr4.2:19743737-19743756 MS.gene90204:CDS 35.0%
TCAGAAAAGGATTGTCTACT+TGG - chr4.2:19744129-19744148 None:intergenic 35.0%
TGAGTATCTTGAAATGACTG+AGG + chr4.2:19743146-19743165 MS.gene90204:CDS 35.0%
TGAGTGTATGTTTGGTATCA+TGG - chr4.2:19742724-19742743 None:intergenic 35.0%
TGTATGTTTGGTATCATGGT+CGG - chr4.2:19742720-19742739 None:intergenic 35.0%
TTTCCACTTCTAGATAGCAA+AGG - chr4.2:19742964-19742983 None:intergenic 35.0%
! ACAGGAAAACACTTCTAGAA+GGG + chr4.2:19745242-19745261 MS.gene90204:CDS 35.0%
! ACTGATTTAGAGAAAAGGGT+TGG + chr4.2:19743967-19743986 MS.gene90204:CDS 35.0%
! ATTTGAAGATTTTCTGCCCA+CGG - chr4.2:19743282-19743301 None:intergenic 35.0%
! CTTCTAGTTGTCTTTCTGTT+CGG - chr4.2:19745517-19745536 None:intergenic 35.0%
! TATCCTTTGCTATCTAGAAG+TGG + chr4.2:19742958-19742977 MS.gene90204:CDS 35.0%
! TTTGGAGCTTCAACATGATA+TGG + chr4.2:19745354-19745373 MS.gene90204:CDS 35.0%
! TTTTCTGATCACAAGCATGT+GGG + chr4.2:19744141-19744160 MS.gene90204:CDS 35.0%
!! GTTTCACTTTTCTCAAAGCA+AGG - chr4.2:19744033-19744052 None:intergenic 35.0%
!! GTTTTCCTGTTTGTTACCAT+TGG - chr4.2:19745232-19745251 None:intergenic 35.0%
!!! GCTATGTTGTTTTGTCTTCA+TGG + chr4.2:19743214-19743233 MS.gene90204:CDS 35.0%
AAGGTAGGCTGTGTTAGATA+TGG + chr4.2:19743852-19743871 MS.gene90204:intron 40.0%
ACAGAAAGACAACTAGAAGG+TGG + chr4.2:19745518-19745537 MS.gene90204:CDS 40.0%
ACATCTTCAAACCACCAATC+AGG - chr4.2:19743456-19743475 None:intergenic 40.0%
ACCAACCAATGGTAACAAAC+AGG + chr4.2:19745224-19745243 MS.gene90204:CDS 40.0%
ACTTGCACTACTACCCTTAA+TGG - chr4.2:19745130-19745149 None:intergenic 40.0%
AGATTTGAACTGATTGGTGC+TGG + chr4.2:19743537-19743556 MS.gene90204:CDS 40.0%
AGCAACTCCATAGCAGAATT+TGG - chr4.2:19743082-19743101 None:intergenic 40.0%
AGCGGAACAACTTGGTTTAT+AGG - chr4.2:19742632-19742651 None:intergenic 40.0%
ATCCTGACTGGAACAATCTT+TGG + chr4.2:19742780-19742799 MS.gene90204:intron 40.0%
ATGACTGAGGATCTAGAAGA+AGG + chr4.2:19743159-19743178 MS.gene90204:CDS 40.0%
ATGATGTGGATCTTGTTCAG+AGG + chr4.2:19744058-19744077 MS.gene90204:CDS 40.0%
ATTATAAGGACCTGGCAATC+CGG - chr4.2:19742835-19742854 None:intergenic 40.0%
CAACACTAACTGAGCATGAA+AGG + chr4.2:19745026-19745045 MS.gene90204:CDS 40.0%
CACATGCTTGTGATCAGAAA+AGG - chr4.2:19744142-19744161 None:intergenic 40.0%
CATCCTTCTTGGAAAGATTG+CGG - chr4.2:19745271-19745290 None:intergenic 40.0%
CCTGAAACTTTGTCGATGAA+AGG - chr4.2:19744242-19744261 None:intergenic 40.0%
CCTTTCATCGACAAAGTTTC+AGG + chr4.2:19744239-19744258 MS.gene90204:CDS 40.0%
CGAACAGAAAGACAACTAGA+AGG + chr4.2:19745515-19745534 MS.gene90204:CDS 40.0%
CTGTTCGGTTCTTCAGAAAT+TGG - chr4.2:19745502-19745521 None:intergenic 40.0%
CTTAACACCATGTTTGGTTG+AGG - chr4.2:19742280-19742299 None:intergenic 40.0%
CTTTCATGCTCAGTTAGTGT+TGG - chr4.2:19745028-19745047 None:intergenic 40.0%
GAAAAGATACCTTGTGCAAG+TGG - chr4.2:19742527-19742546 None:intergenic 40.0%
TATGCAACTAAATGGCTACC+AGG + chr4.2:19743570-19743589 MS.gene90204:CDS 40.0%
TGAATCTCAGTACCAACCAA+TGG + chr4.2:19745213-19745232 MS.gene90204:CDS 40.0%
TGTGGACAGTTATCTTACAG+AGG + chr4.2:19744200-19744219 MS.gene90204:CDS 40.0%
TTCATCAAAGAGGCAACTCA+TGG + chr4.2:19742310-19742329 MS.gene90204:CDS 40.0%
TTGACAGCAAGCAACATTTC+CGG + chr4.2:19743108-19743127 MS.gene90204:CDS 40.0%
! ATTTCCTCTCTTTTGCATGC+AGG + chr4.2:19742445-19742464 MS.gene90204:intron 40.0%
! CTTTTCTGATCACAAGCATG+TGG + chr4.2:19744140-19744159 MS.gene90204:CDS 40.0%
! TCCTGTTTGTTACCATTGGT+TGG - chr4.2:19745228-19745247 None:intergenic 40.0%
!! TGGTCGGTTTGTTAGAATCA+CGG - chr4.2:19742704-19742723 None:intergenic 40.0%
AAAGTGAAACAGCAGCTGGA+AGG + chr4.2:19742232-19742251 MS.gene90204:CDS 45.0%
AACTAAATGGCTACCAGGAC+TGG + chr4.2:19743575-19743594 MS.gene90204:CDS 45.0%
AAGTGAAACAGCAGCTGGAA+GGG + chr4.2:19742233-19742252 MS.gene90204:CDS 45.0%
ACAACTTGAGCGGAACAACT+TGG - chr4.2:19742640-19742659 None:intergenic 45.0%
ACACCGCAATCTTTCCAAGA+AGG + chr4.2:19745265-19745284 MS.gene90204:CDS 45.0%
AGACTTTACTTGTCTCCTGG+CGG + chr4.2:19742805-19742824 MS.gene90204:intron 45.0%
ATAGCTATGGATGATCTCCC+TGG + chr4.2:19743030-19743049 MS.gene90204:CDS 45.0%
CATACCTGCATGCAAAAGAG+AGG - chr4.2:19742452-19742471 None:intergenic 45.0%
CGAAAGCTTCAAGCCCAAAA+TGG + chr4.2:19743385-19743404 MS.gene90204:CDS 45.0%
CGCAATCTTTCCAAGAAGGA+TGG + chr4.2:19745269-19745288 MS.gene90204:CDS 45.0%
CTAGCCAACAGCTCTCTAAA+AGG + chr4.2:19745184-19745203 MS.gene90204:CDS 45.0%
CTATAGACTCGCTGCATCTT+CGG - chr4.2:19743308-19743327 None:intergenic 45.0%
CTTGCAGCCAAATTCTGCTA+TGG + chr4.2:19743072-19743091 MS.gene90204:CDS 45.0%
GATACTCAGCAGCACATCTT+AGG - chr4.2:19743134-19743153 None:intergenic 45.0%
GCTGCAAGTTCAAATGCTTC+AGG - chr4.2:19743060-19743079 None:intergenic 45.0%
GGATCTTGTTCAGAGGTTGT+TGG + chr4.2:19744065-19744084 MS.gene90204:CDS 45.0%
GTTCTCACCTCAACCAAACA+TGG + chr4.2:19742270-19742289 MS.gene90204:CDS 45.0%
TAGCTGTTGTCCATCCTTCT+TGG - chr4.2:19745282-19745301 None:intergenic 45.0%
TCAGAAAGTGAAACAGCAGC+TGG + chr4.2:19742228-19742247 MS.gene90204:CDS 45.0%
TCCAGTCAGGATTCGAACTT+GGG - chr4.2:19742772-19742791 None:intergenic 45.0%
TCTTCAAACCACCAATCAGG+AGG - chr4.2:19743453-19743472 None:intergenic 45.0%
TGCAGAAGCTTTACCTGAGT+CGG + chr4.2:19744266-19744285 MS.gene90204:CDS 45.0%
TTCCAGTCAGGATTCGAACT+TGG - chr4.2:19742773-19742792 None:intergenic 45.0%
! AGCAAAAGCACACATCAGCT+TGG + chr4.2:19745410-19745429 MS.gene90204:CDS 45.0%
! TACGCCCACCTTATTCCTTT+TGG - chr4.2:19743351-19743370 None:intergenic 45.0%
! TGTTGCTTGTTTCCTCTCCT+TGG - chr4.2:19743620-19743639 None:intergenic 45.0%
!! ACACATCAGCTTGGACTAGT+GGG + chr4.2:19745419-19745438 MS.gene90204:CDS 45.0%
!! CGGTTTGTTAGAATCACGGT+GGG - chr4.2:19742700-19742719 None:intergenic 45.0%
!! TCGGTTTGTTAGAATCACGG+TGG - chr4.2:19742701-19742720 None:intergenic 45.0%
AACAACAGTAGCTGGAGAGG+TGG + chr4.2:19743669-19743688 MS.gene90204:CDS 50.0%
ACAGCAGCTGGAAGGGAATA+TGG + chr4.2:19742240-19742259 MS.gene90204:CDS 50.0%
ACCCAAGTTCGAATCCTGAC+TGG + chr4.2:19742768-19742787 MS.gene90204:intron 50.0%
AGCAGCTGGAAGGGAATATG+GGG + chr4.2:19742242-19742261 MS.gene90204:CDS 50.0%
ATCAGCTAGTGCCTCCTGAT+TGG + chr4.2:19743439-19743458 MS.gene90204:CDS 50.0%
CACGAGACACTGTCTTTCTG+CGG - chr4.2:19743792-19743811 None:intergenic 50.0%
CAGCAGCTGGAAGGGAATAT+GGG + chr4.2:19742241-19742260 MS.gene90204:CDS 50.0%
CCATTCTCAGAAGCCTGAGA+AGG - chr4.2:19743821-19743840 None:intergenic 50.0%
CGACACTACAATCCAAGGAG+AGG + chr4.2:19743605-19743624 MS.gene90204:CDS 50.0%
CTATGGATGATCTCCCTGGA+GGG + chr4.2:19743034-19743053 MS.gene90204:CDS 50.0%
GACAACAACAGTAGCTGGAG+AGG + chr4.2:19743666-19743685 MS.gene90204:CDS 50.0%
GATGCAGCGAGTCTATAGCT+TGG + chr4.2:19743310-19743329 MS.gene90204:CDS 50.0%
GCCAGACTTTACTTGTCTCC+TGG + chr4.2:19742802-19742821 MS.gene90204:intron 50.0%
GCCAGGAGACAAGTAAAGTC+TGG - chr4.2:19742806-19742825 None:intergenic 50.0%
GCTGCATTCACGGTAGAATC+AGG - chr4.2:19744752-19744771 None:intergenic 50.0%
GGAATAAGGTGGGCGTATAC+CGG + chr4.2:19743354-19743373 MS.gene90204:CDS 50.0%
GGCCAAAGATTGTTCCAGTC+AGG - chr4.2:19742785-19742804 None:intergenic 50.0%
GGGAACGACACTACAATCCA+AGG + chr4.2:19743600-19743619 MS.gene90204:CDS 50.0%
TAAATGGCTACCAGGACTGG+CGG + chr4.2:19743578-19743597 MS.gene90204:CDS 50.0%
TACCTTGTGCAAGTGGAAGC+TGG - chr4.2:19742520-19742539 None:intergenic 50.0%
TCGGCTAGAGTTTCTGACGA+TGG + chr4.2:19744285-19744304 MS.gene90204:CDS 50.0%
TCTCGTGTAGCTTCCTTCTC+AGG + chr4.2:19743805-19743824 MS.gene90204:CDS 50.0%
TCTGATCACAAGCATGTGGG+AGG + chr4.2:19744144-19744163 MS.gene90204:CDS 50.0%
TGAAGCTTTCGTCGCTCTTC+CGG - chr4.2:19743376-19743395 None:intergenic 50.0%
! ATTTTGAGCTGCGTGCATGC+AGG - chr4.2:19745098-19745117 None:intergenic 50.0%
! CACACATCAGCTTGGACTAG+TGG + chr4.2:19745418-19745437 MS.gene90204:CDS 50.0%
! CCTTCTCAGGCTTCTGAGAA+TGG + chr4.2:19743818-19743837 MS.gene90204:CDS 50.0%
! CTCTGAAAAGCTGTCACCGT+GGG + chr4.2:19743263-19743282 MS.gene90204:CDS 50.0%
! GAAGCGTCTCTGTCGTGTAA+TGG + chr4.2:19745048-19745067 MS.gene90204:CDS 50.0%
! GGAAGCTGGCTTCTCCAATT+TGG - chr4.2:19742506-19742525 None:intergenic 50.0%
! TCAGAAACTCTAGCCGACTC+AGG - chr4.2:19744282-19744301 None:intergenic 50.0%
!! GCTGCCTTTTAGAGAGCTGT+TGG - chr4.2:19745191-19745210 None:intergenic 50.0%
AAGCCTTGCCACTCTTGCCT+TGG - chr4.2:19744181-19744200 None:intergenic 55.0%
AGCCAGCTTCCACTTGCACA+AGG + chr4.2:19742515-19742534 MS.gene90204:CDS 55.0%
AGCTAGTGCCTCCTGATTGG+TGG + chr4.2:19743442-19743461 MS.gene90204:CDS 55.0%
ATGCCAAGGCAAGAGTGGCA+AGG + chr4.2:19744175-19744194 MS.gene90204:CDS 55.0%
CAAATGCTTCAGGCCCTCCA+GGG - chr4.2:19743050-19743069 None:intergenic 55.0%
CCACTCTTGCCTTGGCATTC+AGG - chr4.2:19744173-19744192 None:intergenic 55.0%
CCTGAATGCCAAGGCAAGAG+TGG + chr4.2:19744170-19744189 MS.gene90204:CDS 55.0%
CGGGGTCGCGAGAATCATAT+AGG - chr4.2:19742999-19743018 None:intergenic 55.0%
CTCAGCAGCACATCTTAGGC+CGG - chr4.2:19743130-19743149 None:intergenic 55.0%
GAGACACTGTCTTTCTGCGG+CGG - chr4.2:19743789-19743808 None:intergenic 55.0%
GCAGCTGGAAGGGAATATGG+GGG + chr4.2:19742243-19742262 MS.gene90204:CDS 55.0%
GCGGTGACAACAACAGTAGC+TGG + chr4.2:19743661-19743680 MS.gene90204:CDS 55.0%
GCTATGGATGATCTCCCTGG+AGG + chr4.2:19743033-19743052 MS.gene90204:CDS 55.0%
GGGAGGTAACCTGAATGCCA+AGG + chr4.2:19744161-19744180 MS.gene90204:CDS 55.0%
TCAAATGCTTCAGGCCCTCC+AGG - chr4.2:19743051-19743070 None:intergenic 55.0%
TGAAAGACTCAAGCCCGAGC+AGG + chr4.2:19743415-19743434 MS.gene90204:CDS 55.0%
! AAATGGCTACCAGGACTGGC+GGG + chr4.2:19743579-19743598 MS.gene90204:CDS 55.0%
! GCACTAGCTGATTCCTGCTC+GGG - chr4.2:19743431-19743450 None:intergenic 55.0%
! GGCACTAGCTGATTCCTGCT+CGG - chr4.2:19743432-19743451 None:intergenic 55.0%
!! CATCAGCTTGGACTAGTGGG+TGG + chr4.2:19745422-19745441 MS.gene90204:CDS 55.0%
!! GCTCTGAAAAGCTGTCACCG+TGG + chr4.2:19743262-19743281 MS.gene90204:CDS 55.0%
CTTGTCTCCTGGCGGAACTC+CGG + chr4.2:19742813-19742832 MS.gene90204:intron 60.0%
GCTGGAAGGGAATATGGGGG+TGG + chr4.2:19742246-19742265 MS.gene90204:CDS 60.0%
GGCAAGAGTGGCAAGGCTTG+TGG + chr4.2:19744182-19744201 MS.gene90204:CDS 60.0%
TGCGTGCATGCAGGCGTCAA+TGG - chr4.2:19745089-19745108 None:intergenic 60.0%
! AATGGCTACCAGGACTGGCG+GGG + chr4.2:19743580-19743599 MS.gene90204:CDS 60.0%
TGGCAATCCGGAGTTCCGCC+AGG - chr4.2:19742823-19742842 None:intergenic 65.0%
TGGCGGAACTCCGGATTGCC+AGG + chr4.2:19742822-19742841 MS.gene90204:intron 65.0%
! GTGTCGTTCCCCGCCAGTCC+TGG - chr4.2:19743591-19743610 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 19742219 19745558 19742219 ID=MS.gene90204
chr4.2 mRNA 19742219 19745558 19742219 ID=MS.gene90204.t1;Parent=MS.gene90204
chr4.2 exon 19742219 19742331 19742219 ID=MS.gene90204.t1.exon1;Parent=MS.gene90204.t1
chr4.2 CDS 19742219 19742331 19742219 ID=cds.MS.gene90204.t1;Parent=MS.gene90204.t1
chr4.2 exon 19742467 19742536 19742467 ID=MS.gene90204.t1.exon2;Parent=MS.gene90204.t1
chr4.2 CDS 19742467 19742536 19742467 ID=cds.MS.gene90204.t1;Parent=MS.gene90204.t1
chr4.2 exon 19742958 19743854 19742958 ID=MS.gene90204.t1.exon3;Parent=MS.gene90204.t1
chr4.2 CDS 19742958 19743854 19742958 ID=cds.MS.gene90204.t1;Parent=MS.gene90204.t1
chr4.2 exon 19743949 19744338 19743949 ID=MS.gene90204.t1.exon4;Parent=MS.gene90204.t1
chr4.2 CDS 19743949 19744338 19743949 ID=cds.MS.gene90204.t1;Parent=MS.gene90204.t1
chr4.2 exon 19745019 19745558 19745019 ID=MS.gene90204.t1.exon5;Parent=MS.gene90204.t1
chr4.2 CDS 19745019 19745558 19745019 ID=cds.MS.gene90204.t1;Parent=MS.gene90204.t1
Gene Sequence

>MS.gene90204

ATGTGGGAATCAGAAAGTGAAACAGCAGCTGGAAGGGAATATGGGGGTGGAGTTCTCACCTCAACCAAACATGGTGTTAAGATTGAAGGTTTTCATCAAAGAGGCAACTCATGGTATGTTAATGTTTAATTCAATGCAGAAAATCATTATTGAAACTTCTAAAATGATCATAATATTTTCTTTTTTTGAACTTTTTATTTATCCATTTTATGTAAGTTAATTGAACATTTCCTCTCTTTTGCATGCAGGTATGTTTCAGCTGATATTCCAAGTGATCTTCAAGTCCAAATTGGAGAAGCCAGCTTCCACTTGCACAAGGTATCTTTTCTTTTCATATTTCATGTAATTTTTTTTTCTTTCCTAGAAAGTTATTTTATTTTTTCTCTAAGGATGATAAGATATACTGCCAACCTATAAACCAAGTTGTTCCGCTCAAGTTGTTAATGAGTTTCAGTGATGTTTGGTATCAAGTTGGACTCCCACCGTGATTCTAACAAACCGACCATGATACCAAACATACACTCAATTAAGGATGATTCATTTGGGAGAACCCAAGTTCGAATCCTGACTGGAACAATCTTTGGCCAGACTTTACTTGTCTCCTGGCGGAACTCCGGATTGCCAGGTCCTTATAATCTATAAACCGAAAGGTTAAGAAAAAAACTACTACTGATCTGTACTGTATTTGAACTGTATTGTGTTGAATTTGATGAACTTTTCTTCCTTTTTGTTGTGAAAGTATCCTTTGCTATCTAGAAGTGGAAAGCTGAACAGAATCCTATATGATTCTCGCGACCCCGACTTGAATAAGATAGCTATGGATGATCTCCCTGGAGGGCCTGAAGCATTTGAACTTGCAGCCAAATTCTGCTATGGAGTTGCTATTGATTTGACAGCAAGCAACATTTCCGGCCTAAGATGTGCTGCTGAGTATCTTGAAATGACTGAGGATCTAGAAGAAGGAAATCTTATATTCAAGACTGAAGCTTTTCTTAGCTATGTTGTTTTGTCTTCATGGAGAGACTCTATAGTAGTGTTGAAAAGCTCTGAAAAGCTGTCACCGTGGGCAGAAAATCTTCAAATTGTCCGAAGATGCAGCGAGTCTATAGCTTGGAAAGCTTGTGCTAATCCAAAAGGAATAAGGTGGGCGTATACCGGAAGAGCGACGAAAGCTTCAAGCCCAAAATGGAACGAAATGAAAGACTCAAGCCCGAGCAGGAATCAGCTAGTGCCTCCTGATTGGTGGTTTGAAGATGTTTCAATCCTTAGGATTGATCACTTTGTTAGAGTCATTACTGCTATTAAGGTAAAAGGTATGAGATTTGAACTGATTGGTGCTGGAATAATGCATTATGCAACTAAATGGCTACCAGGACTGGCGGGGAACGACACTACAATCCAAGGAGAGGAAACAAGCAACAACAGTAATAGTAGTTTTAGTAGCGGTGACAACAACAGTAGCTGGAGAGGTGGACTTCATATGATTGTTTCTGGAACTAGAGATGAAACTTCAAGTCTTCAAACCAAGGATCAAAAGATGGTTATTGAGAGTCTCATCAGCATAATTCCGCCGCAGAAAGACAGTGTCTCGTGTAGCTTCCTTCTCAGGCTTCTGAGAATGGCAAACATGTTAAAGGTAGGCTGTGTTAGATATGGCAACCATAACTAACTTGTAACTACCTTAAACAATGTCTAACTAATTCTAACAAATATCAAACTATCTCTAACAGGTAGCATATGCATTGATTACTGATTTAGAGAAAAGGGTTGGAATGCAGTTTGAGCAAGCTACACTTTCTGATCTTCTAATTCCTTGCTTTGAGAAAAGTGAAACTATGTATGATGTGGATCTTGTTCAGAGGTTGTTGGAGCATTTTCTTGTTCAAGAACAAACTGAAGGTTATAGTCCAAGTAGACAATCCTTTTCTGATCACAAGCATGTGGGAGGTAACCTGAATGCCAAGGCAAGAGTGGCAAGGCTTGTGGACAGTTATCTTACAGAGGTATCAAGAGATAGAAACCTTTCATCGACAAAGTTTCAGGTTCTTGCAGAAGCTTTACCTGAGTCGGCTAGAGTTTCTGACGATGGACTATATAGAGCAATTGATTCATATCTCAAGGTAATTAATCACTTTTCAAAGGATAAAGGGGAACAATCTTTCAAGTTTCTTATCATTTTTTATTTTCAATTACTCTAGGATACATGAACTTTAAGTAAAATTTGAGTCAGAACTTGGAGTTGCATAATATGCTCATTATACACATCTTAAAAGCAAAGTTGTATTGGTTTTGGCATCTTTGTTTAAGAAATGTGCATATATGCACATTCTGAAATATGACAATATACTTATTTAGGTTCTATAATTAATTGAACACTGAATTTATGTACTTTATGTGCTAATATTTCAAGATAACATTGGAGGAAGCGCTATTATATCCCTTCATCGCAATTATATCTTTACGACAAGATTACAAAAGCCTCAAATTTCATTTTCAATGAGATTACATTTGACTTACAGAATAAACTTTTCCTGATTCTACCGTGAATGCAGCTTCTAGTATATAGTGGTATGGTATTTACAAAAGATATGAATTTAGTAAACACATAATCAATCATTTCTCTAGAAAAGATTAGCTATATTTGTCATTGTTTGAAATAATAAATGCACCAATTTCATTGATGTCTTATGTACTCATTTTAGAAGAATGAGTCGGAACAATTAGTATAGTCTAGATTCACATGATATGATCATGGATTTATATTTCTTTACTTCTGATGATCTGTGAAACAAATTAACAGGCACATCCAACACTAACTGAGCATGAAAGGAAGCGTCTCTGTCGTGTAATGGATTGTCAAAAACTCTCCATTGACGCCTGCATGCACGCAGCTCAAAATGAACGACTGCCATTAAGGGTAGTAGTGCAAGTTCTATTCTCTGAACAAGTAAAGATAAGCAACGCACTAGCCAACAGCTCTCTAAAAGGCAGCGTTGAATCTCAGTACCAACCAATGGTAACAAACAGGAAAACACTTCTAGAAGGGACACCGCAATCTTTCCAAGAAGGATGGACAACAGCTAAGAAAGACATTAACACATTAAAGTTTGAACTTGAGAGTGTGAAGACAAAATATTTGGAGCTTCAACATGATATGGAAAATCTGCAGAAAAATTTCGATAAGCTGTTGAAGCAAAAGCACACATCAGCTTGGACTAGTGGGTGGAAGAAACTGAGCAAACTTACTAAAATGACAAATGTGGAAAATCATGATATGGTTCCAATTTCTGAAGAACCGAACAGAAAGACAACTAGAAGGTGGAGAAACTCAATATCTTGA
Protein sequence

>MS.gene90204.t1

MWESESETAAGREYGGGVLTSTKHGVKIEGFHQRGNSWYVSADIPSDLQVQIGEASFHLHKYPLLSRSGKLNRILYDSRDPDLNKIAMDDLPGGPEAFELAAKFCYGVAIDLTASNISGLRCAAEYLEMTEDLEEGNLIFKTEAFLSYVVLSSWRDSIVVLKSSEKLSPWAENLQIVRRCSESIAWKACANPKGIRWAYTGRATKASSPKWNEMKDSSPSRNQLVPPDWWFEDVSILRIDHFVRVITAIKVKGMRFELIGAGIMHYATKWLPGLAGNDTTIQGEETSNNSNSSFSSGDNNSSWRGGLHMIVSGTRDETSSLQTKDQKMVIESLISIIPPQKDSVSCSFLLRLLRMANMLKVAYALITDLEKRVGMQFEQATLSDLLIPCFEKSETMYDVDLVQRLLEHFLVQEQTEGYSPSRQSFSDHKHVGGNLNAKARVARLVDSYLTEVSRDRNLSSTKFQVLAEALPESARVSDDGLYRAIDSYLKAHPTLTEHERKRLCRVMDCQKLSIDACMHAAQNERLPLRVVVQVLFSEQVKISNALANSSLKGSVESQYQPMVTNRKTLLEGTPQSFQEGWTTAKKDINTLKFELESVKTKYLELQHDMENLQKNFDKLLKQKHTSAWTSGWKKLSKLTKMTNVENHDMVPISEEPNRKTTRRWRNSIS