Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006756.t1 | XP_003607686.1 | 87.7 | 227 | 23 | 2 | 1 | 225 | 1 | 224 | 1.40E-102 | 382.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006756.t1 | O48915 | 39.4 | 193 | 90 | 6 | 11 | 190 | 13 | 191 | 1.5e-28 | 127.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006756.t1 | G7JF17 | 87.7 | 227 | 23 | 2 | 1 | 225 | 1 | 224 | 9.8e-103 | 382.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006756 | MS.gene99821 | PPI |
| MS.gene031547 | MS.gene006756 | PPI |
| MS.gene006756 | MS.gene031261 | PPI |
| MS.gene69812 | MS.gene006756 | PPI |
| MS.gene96823 | MS.gene006756 | PPI |
| MS.gene006756 | MS.gene87282 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006756.t1 | MTR_4g081200 | 93.392 | 227 | 10 | 3 | 1 | 225 | 1 | 224 | 7.13e-152 | 421 |
| MS.gene006756.t1 | MTR_2g082990 | 49.738 | 191 | 86 | 3 | 11 | 197 | 8 | 192 | 2.38e-58 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene006756.t1 | AT3G20600 | 41.451 | 193 | 86 | 6 | 11 | 190 | 13 | 191 | 9.38e-42 | 141 |
| MS.gene006756.t1 | AT3G20590 | 39.899 | 198 | 97 | 6 | 4 | 190 | 6 | 192 | 1.71e-34 | 124 |
| MS.gene006756.t1 | AT3G20610 | 36.757 | 185 | 94 | 7 | 25 | 197 | 26 | 199 | 2.96e-27 | 104 |
Find 62 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTCTGGGTTGCTCTTTC+TGG | 0.060042 | 4.1:+25666290 | MS.gene006756:CDS |
| CTGCCCCGTTTCGCGGGTTC+TGG | 0.069782 | 4.1:+25666276 | MS.gene006756:CDS |
| CAACCCAGAACCCGCGAAAC+GGG | 0.291265 | 4.1:-25666280 | None:intergenic |
| TTACCAAGCCTGATCCCTTT+AGG | 0.299297 | 4.1:-25666227 | None:intergenic |
| AGTGATTGAATGAAGCATTT+TGG | 0.304303 | 4.1:-25665789 | None:intergenic |
| TGCCCCGTTTCGCGGGTTCT+GGG | 0.318361 | 4.1:+25666277 | MS.gene006756:CDS |
| GTTGTTAGAGTGTTTGTGAT+TGG | 0.357570 | 4.1:-25665847 | None:intergenic |
| AGACACCTTTGTCTTCGTTT+TGG | 0.361673 | 4.1:-25665908 | None:intergenic |
| GCTTCAGACCCGACCCGAAC+TGG | 0.379399 | 4.1:-25666254 | None:intergenic |
| TCTGAAGCTGCCCCGTTTCG+CGG | 0.386385 | 4.1:+25666269 | MS.gene006756:CDS |
| GCAGGTGCAGCAGTTGATGC+GGG | 0.394326 | 4.1:-25665697 | None:intergenic |
| GGCTTGGTAATGCTCCAGTT+CGG | 0.396045 | 4.1:+25666240 | MS.gene006756:CDS |
| GCTTGGTAATGCTCCAGTTC+GGG | 0.402093 | 4.1:+25666241 | MS.gene006756:CDS |
| GTGGAACTTAATGTCAGTTC+AGG | 0.409244 | 4.1:+25666191 | MS.gene006756:CDS |
| ACTAAACGACACCGTTTGTC+TGG | 0.423254 | 4.1:+25666158 | MS.gene006756:CDS |
| TCTGCCGAGGCACGTGGTGG+TGG | 0.430137 | 4.1:+25666053 | MS.gene006756:CDS |
| TTGATGCGGGGTTCCTGCGC+GGG | 0.451168 | 4.1:-25665684 | None:intergenic |
| CTGAAGCTGCCCCGTTTCGC+GGG | 0.451197 | 4.1:+25666270 | MS.gene006756:CDS |
| CACTCCACCACCACGTGCCT+CGG | 0.465177 | 4.1:-25666057 | None:intergenic |
| GCAACCCAGAACCCGCGAAA+CGG | 0.474820 | 4.1:-25666281 | None:intergenic |
| GTAATGCTCCAGTTCGGGTC+GGG | 0.485181 | 4.1:+25666246 | MS.gene006756:CDS |
| GAGTTCGGAGTGTGAGCCAC+AGG | 0.493976 | 4.1:-25665761 | None:intergenic |
| AGGTGAATCCTAAAGGGATC+AGG | 0.497883 | 4.1:+25666219 | MS.gene006756:CDS |
| GGTCAGAAGGTGAATCCTAA+AGG | 0.505752 | 4.1:+25666212 | MS.gene006756:CDS |
| GTCAGAAGGTGAATCCTAAA+GGG | 0.505982 | 4.1:+25666213 | MS.gene006756:CDS |
| AGCAGGTGCAGCAGTTGATG+CGG | 0.513768 | 4.1:-25665698 | None:intergenic |
| CGTCAACACCACGCGTCCAC+TGG | 0.520956 | 4.1:+25665971 | MS.gene006756:CDS |
| GGTGATGAAGCTAGTGCAGC+AGG | 0.524004 | 4.1:-25665715 | None:intergenic |
| GGTAATGCTCCAGTTCGGGT+CGG | 0.527404 | 4.1:+25666245 | MS.gene006756:CDS |
| AACGACACCGTTTGTCTGGA+GGG | 0.528137 | 4.1:+25666162 | MS.gene006756:CDS |
| GTAGATGGCGCGGCCCGCGC+AGG | 0.530494 | 4.1:+25665671 | None:intergenic |
| GGAGTGTGAGCCACAGGAAG+AGG | 0.532200 | 4.1:-25665755 | None:intergenic |
| GAGGATGGTGAGGCCTGTGC+TGG | 0.544681 | 4.1:-25665736 | None:intergenic |
| CATTTGCCCCTCCAGACAAA+CGG | 0.547066 | 4.1:-25666169 | None:intergenic |
| GAAACCATCGAGAGTGGAGT+TGG | 0.554819 | 4.1:-25665994 | None:intergenic |
| GCCTCACCATCCTCTTCCTG+TGG | 0.560087 | 4.1:+25665745 | MS.gene006756:CDS |
| TAATGTCAGTTCAGGTCAGA+AGG | 0.561149 | 4.1:+25666199 | MS.gene006756:CDS |
| AACCCAGAACCCGCGAAACG+GGG | 0.561738 | 4.1:-25666279 | None:intergenic |
| TTTGTCTGGAGGGGCAAATG+TGG | 0.563437 | 4.1:+25666172 | MS.gene006756:CDS |
| AATCCTAAAGGGATCAGGCT+TGG | 0.567380 | 4.1:+25666224 | MS.gene006756:CDS |
| AAACACTTCCTTGCACTGTA+CGG | 0.569559 | 4.1:-25666082 | None:intergenic |
| TGGGGTTCTGCCGAGGCACG+TGG | 0.580720 | 4.1:+25666047 | MS.gene006756:CDS |
| GGAGTGAACCGTACAGTGCA+AGG | 0.581559 | 4.1:+25666074 | MS.gene006756:CDS |
| ACTAGCTTCATCACCAGCAC+AGG | 0.582415 | 4.1:+25665723 | MS.gene006756:CDS |
| TGAGAAATGGGGTTCTGCCG+AGG | 0.598645 | 4.1:+25666040 | MS.gene006756:CDS |
| CAGGTGCAGCAGTTGATGCG+GGG | 0.599703 | 4.1:-25665696 | None:intergenic |
| TTGGTAGAAACCATCGAGAG+TGG | 0.602681 | 4.1:-25666000 | None:intergenic |
| CTGGCCAACTCCACTCTCGA+TGG | 0.603518 | 4.1:+25665990 | MS.gene006756:CDS |
| GACGCGTGGTGTTGACGTCG+AGG | 0.608775 | 4.1:-25665965 | None:intergenic |
| AAACGACACCGTTTGTCTGG+AGG | 0.617610 | 4.1:+25666161 | MS.gene006756:CDS |
| GGAGTTGGCCAGTGGACGCG+TGG | 0.622642 | 4.1:-25665979 | None:intergenic |
| GGTTCTGCCGAGGCACGTGG+TGG | 0.625771 | 4.1:+25666050 | MS.gene006756:CDS |
| TCGAGAGTGGAGTTGGCCAG+TGG | 0.626154 | 4.1:-25665987 | None:intergenic |
| GAGAGCAAATGTAAGACGAA+TGG | 0.627099 | 4.1:-25665940 | None:intergenic |
| TGTGAGCCACAGGAAGAGGA+TGG | 0.629132 | 4.1:-25665751 | None:intergenic |
| GTTGATGCGGGGTTCCTGCG+CGG | 0.633498 | 4.1:-25665685 | None:intergenic |
| ATCAACCACCGTAGATGGCG+CGG | 0.634709 | 4.1:+25665661 | None:intergenic |
| GCCACAGGAAGAGGATGGTG+AGG | 0.648443 | 4.1:-25665746 | None:intergenic |
| ACGACACCGTTTGTCTGGAG+GGG | 0.667289 | 4.1:+25666163 | MS.gene006756:CDS |
| ACTCTCGATGGTTTCTACCA+AGG | 0.673268 | 4.1:+25666002 | MS.gene006756:CDS |
| GATGGTTTCTACCAAGGACA+TGG | 0.676986 | 4.1:+25666008 | MS.gene006756:CDS |
| GCAAACCAAAACGAAGACAA+AGG | 0.684119 | 4.1:+25665903 | MS.gene006756:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTGATTGAATGAAGCATTT+TGG | - | chr4.1:25665792-25665811 | None:intergenic | 30.0% |
| ATGGTAAAAACGCTGAGAAA+TGG | + | chr4.1:25666027-25666046 | MS.gene006756:CDS | 35.0% | |
| TGGTAAAAACGCTGAGAAAT+GGG | + | chr4.1:25666028-25666047 | MS.gene006756:CDS | 35.0% | |
| ! | GTTGTTAGAGTGTTTGTGAT+TGG | - | chr4.1:25665850-25665869 | None:intergenic | 35.0% |
| !! | TGGAACTTTTGTTTCTCTCT+TGG | + | chr4.1:25666310-25666329 | MS.gene006756:CDS | 35.0% |
| !!! | AGCATTTTGGTTGTTGAGTT+CGG | - | chr4.1:25665779-25665798 | None:intergenic | 35.0% |
| !!! | GCAAGGAAGTGTTTTCTTAA+GGG | + | chr4.1:25666091-25666110 | MS.gene006756:CDS | 35.0% |
| !!! | TGCAAGGAAGTGTTTTCTTA+AGG | + | chr4.1:25666090-25666109 | MS.gene006756:CDS | 35.0% |
| AAACACTTCCTTGCACTGTA+CGG | - | chr4.1:25666085-25666104 | None:intergenic | 40.0% | |
| GAGAGCAAATGTAAGACGAA+TGG | - | chr4.1:25665943-25665962 | None:intergenic | 40.0% | |
| GCAAACCAAAACGAAGACAA+AGG | + | chr4.1:25665903-25665922 | MS.gene006756:CDS | 40.0% | |
| GGTAAAAACGCTGAGAAATG+GGG | + | chr4.1:25666029-25666048 | MS.gene006756:CDS | 40.0% | |
| GTCAGAAGGTGAATCCTAAA+GGG | + | chr4.1:25666213-25666232 | MS.gene006756:CDS | 40.0% | |
| GTGGAACTTAATGTCAGTTC+AGG | + | chr4.1:25666191-25666210 | MS.gene006756:CDS | 40.0% | |
| TAATGTCAGTTCAGGTCAGA+AGG | + | chr4.1:25666199-25666218 | MS.gene006756:CDS | 40.0% | |
| ! | AGACACCTTTGTCTTCGTTT+TGG | - | chr4.1:25665911-25665930 | None:intergenic | 40.0% |
| !! | GTGAGTGTTTTGTTGAGAGA+AGG | - | chr4.1:25665822-25665841 | None:intergenic | 40.0% |
| !! | GTGTTTTGTTGAGAGAAGGA+AGG | - | chr4.1:25665818-25665837 | None:intergenic | 40.0% |
| !!! | AGGAAGTGTTTTCTTAAGGG+TGG | + | chr4.1:25666094-25666113 | MS.gene006756:CDS | 40.0% |
| !!! | CGTTTTGGTTTGCTAGTTTG+AGG | - | chr4.1:25665896-25665915 | None:intergenic | 40.0% |
| AATCCTAAAGGGATCAGGCT+TGG | + | chr4.1:25666224-25666243 | MS.gene006756:CDS | 45.0% | |
| ACTAAACGACACCGTTTGTC+TGG | + | chr4.1:25666158-25666177 | MS.gene006756:CDS | 45.0% | |
| ACTCTCGATGGTTTCTACCA+AGG | + | chr4.1:25666002-25666021 | MS.gene006756:CDS | 45.0% | |
| AGGTGAATCCTAAAGGGATC+AGG | + | chr4.1:25666219-25666238 | MS.gene006756:CDS | 45.0% | |
| GATGGTTTCTACCAAGGACA+TGG | + | chr4.1:25666008-25666027 | MS.gene006756:CDS | 45.0% | |
| GGTCAGAAGGTGAATCCTAA+AGG | + | chr4.1:25666212-25666231 | MS.gene006756:CDS | 45.0% | |
| TTACCAAGCCTGATCCCTTT+AGG | - | chr4.1:25666230-25666249 | None:intergenic | 45.0% | |
| TTGGTAGAAACCATCGAGAG+TGG | - | chr4.1:25666003-25666022 | None:intergenic | 45.0% | |
| ! | CAGCGTTTTTACCATGTCCT+TGG | - | chr4.1:25666022-25666041 | None:intergenic | 45.0% |
| AAACGACACCGTTTGTCTGG+AGG | + | chr4.1:25666161-25666180 | MS.gene006756:CDS | 50.0% | |
| AACGACACCGTTTGTCTGGA+GGG | + | chr4.1:25666162-25666181 | MS.gene006756:CDS | 50.0% | |
| CATTTGCCCCTCCAGACAAA+CGG | - | chr4.1:25666172-25666191 | None:intergenic | 50.0% | |
| GAAACCATCGAGAGTGGAGT+TGG | - | chr4.1:25665997-25666016 | None:intergenic | 50.0% | |
| TTTGTCTGGAGGGGCAAATG+TGG | + | chr4.1:25666172-25666191 | MS.gene006756:CDS | 50.0% | |
| ! | ACTAGCTTCATCACCAGCAC+AGG | + | chr4.1:25665723-25665742 | MS.gene006756:CDS | 50.0% |
| !! | GCTTGGTAATGCTCCAGTTC+GGG | + | chr4.1:25666241-25666260 | MS.gene006756:CDS | 50.0% |
| !! | GGCTTGGTAATGCTCCAGTT+CGG | + | chr4.1:25666240-25666259 | MS.gene006756:CDS | 50.0% |
| ACGACACCGTTTGTCTGGAG+GGG | + | chr4.1:25666163-25666182 | MS.gene006756:CDS | 55.0% | |
| GGAGTGAACCGTACAGTGCA+AGG | + | chr4.1:25666074-25666093 | MS.gene006756:CDS | 55.0% | |
| GGTAATGCTCCAGTTCGGGT+CGG | + | chr4.1:25666245-25666264 | MS.gene006756:CDS | 55.0% | |
| GGTGATGAAGCTAGTGCAGC+AGG | - | chr4.1:25665718-25665737 | None:intergenic | 55.0% | |
| GTAATGCTCCAGTTCGGGTC+GGG | + | chr4.1:25666246-25666265 | MS.gene006756:CDS | 55.0% | |
| TGAGAAATGGGGTTCTGCCG+AGG | + | chr4.1:25666040-25666059 | MS.gene006756:CDS | 55.0% | |
| TGTGAGCCACAGGAAGAGGA+TGG | - | chr4.1:25665754-25665773 | None:intergenic | 55.0% | |
| ! | AGCAGGTGCAGCAGTTGATG+CGG | - | chr4.1:25665701-25665720 | None:intergenic | 55.0% |
| !! | GGGTTCTGGGTTGCTCTTTC+TGG | + | chr4.1:25666290-25666309 | MS.gene006756:CDS | 55.0% |
| AACCCAGAACCCGCGAAACG+GGG | - | chr4.1:25666282-25666301 | None:intergenic | 60.0% | |
| CAACCCAGAACCCGCGAAAC+GGG | - | chr4.1:25666283-25666302 | None:intergenic | 60.0% | |
| CTGGCCAACTCCACTCTCGA+TGG | + | chr4.1:25665990-25666009 | MS.gene006756:CDS | 60.0% | |
| GAGTTCGGAGTGTGAGCCAC+AGG | - | chr4.1:25665764-25665783 | None:intergenic | 60.0% | |
| GCAACCCAGAACCCGCGAAA+CGG | - | chr4.1:25666284-25666303 | None:intergenic | 60.0% | |
| GCCACAGGAAGAGGATGGTG+AGG | - | chr4.1:25665749-25665768 | None:intergenic | 60.0% | |
| GCCTCACCATCCTCTTCCTG+TGG | + | chr4.1:25665745-25665764 | MS.gene006756:CDS | 60.0% | |
| GGAGTGTGAGCCACAGGAAG+AGG | - | chr4.1:25665758-25665777 | None:intergenic | 60.0% | |
| TCGAGAGTGGAGTTGGCCAG+TGG | - | chr4.1:25665990-25666009 | None:intergenic | 60.0% | |
| ! | CAGGTGCAGCAGTTGATGCG+GGG | - | chr4.1:25665699-25665718 | None:intergenic | 60.0% |
| ! | GCAGGTGCAGCAGTTGATGC+GGG | - | chr4.1:25665700-25665719 | None:intergenic | 60.0% |
| ! | TCTGAAGCTGCCCCGTTTCG+CGG | + | chr4.1:25666269-25666288 | MS.gene006756:CDS | 60.0% |
| CACTCCACCACCACGTGCCT+CGG | - | chr4.1:25666060-25666079 | None:intergenic | 65.0% | |
| CGTCAACACCACGCGTCCAC+TGG | + | chr4.1:25665971-25665990 | MS.gene006756:CDS | 65.0% | |
| CTGAAGCTGCCCCGTTTCGC+GGG | + | chr4.1:25666270-25666289 | MS.gene006756:CDS | 65.0% | |
| GAGGATGGTGAGGCCTGTGC+TGG | - | chr4.1:25665739-25665758 | None:intergenic | 65.0% | |
| GCTTCAGACCCGACCCGAAC+TGG | - | chr4.1:25666257-25666276 | None:intergenic | 65.0% | |
| GTTGATGCGGGGTTCCTGCG+CGG | - | chr4.1:25665688-25665707 | None:intergenic | 65.0% | |
| TGCCCCGTTTCGCGGGTTCT+GGG | + | chr4.1:25666277-25666296 | MS.gene006756:CDS | 65.0% | |
| TTGATGCGGGGTTCCTGCGC+GGG | - | chr4.1:25665687-25665706 | None:intergenic | 65.0% | |
| !! | GACGCGTGGTGTTGACGTCG+AGG | - | chr4.1:25665968-25665987 | None:intergenic | 65.0% |
| CTGCCCCGTTTCGCGGGTTC+TGG | + | chr4.1:25666276-25666295 | MS.gene006756:CDS | 70.0% | |
| GGAGTTGGCCAGTGGACGCG+TGG | - | chr4.1:25665982-25666001 | None:intergenic | 70.0% | |
| GGTTCTGCCGAGGCACGTGG+TGG | + | chr4.1:25666050-25666069 | MS.gene006756:CDS | 70.0% | |
| TCTGCCGAGGCACGTGGTGG+TGG | + | chr4.1:25666053-25666072 | MS.gene006756:CDS | 70.0% | |
| TGGGGTTCTGCCGAGGCACG+TGG | + | chr4.1:25666047-25666066 | MS.gene006756:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 25665675 | 25666352 | 25665675 | ID=MS.gene006756 |
| chr4.1 | mRNA | 25665675 | 25666352 | 25665675 | ID=MS.gene006756.t1;Parent=MS.gene006756 |
| chr4.1 | exon | 25665675 | 25666352 | 25665675 | ID=MS.gene006756.t1.exon1;Parent=MS.gene006756.t1 |
| chr4.1 | CDS | 25665675 | 25666352 | 25665675 | ID=cds.MS.gene006756.t1;Parent=MS.gene006756.t1 |
| Gene Sequence |
| Protein sequence |
