AlfalfaGEDB | MS.gene96823

M. sativa cultivar XinJiangDaYe / MS.gene96823

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96823.t1	XP_003607686.1	87.7	227	23	2	1	225	1	224	1.40E-102	382.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96823.t1	O48915	39.4	193	90	6	11	190	13	191	1.5e-28	127.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96823.t1	G7JF17	87.7	227	23	2	1	225	1	224	9.8e-103	382.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene96823	MS.gene031261	PPI
MS.gene96823	MS.gene69812	PPI
MS.gene96823	MS.gene031547	PPI
MS.gene96823	MS.gene87282	PPI
MS.gene96823	MS.gene006756	PPI
MS.gene96823	MS.gene99821	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96823.t1	MTR_4g081200	93.392	227	10	3	1	225	1	224	7.13e-152	421
MS.gene96823.t1	MTR_2g082990	49.738	191	86	3	11	197	8	192	2.38e-58	184

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96823.t1	AT3G20600	41.451	193	86	6	11	190	13	191	9.38e-42	141
MS.gene96823.t1	AT3G20590	39.899	198	97	6	4	190	6	192	1.71e-34	124
MS.gene96823.t1	AT3G20610	36.757	185	94	7	25	197	26	199	2.96e-27	104

Find 62 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTTCTGGGTTGCTCTTTC+TGG	0.060042	4.3:+28871844	MS.gene96823:CDS
CTGCCCCGTTTCGCGGGTTC+TGG	0.069782	4.3:+28871830	MS.gene96823:CDS
CAACCCAGAACCCGCGAAAC+GGG	0.291265	4.3:-28871834	None:intergenic
TTACCAAGCCTGATCCCTTT+AGG	0.299297	4.3:-28871781	None:intergenic
AGTGATTGAATGAAGCATTT+TGG	0.304303	4.3:-28871343	None:intergenic
TGCCCCGTTTCGCGGGTTCT+GGG	0.318361	4.3:+28871831	MS.gene96823:CDS
GTTGTTAGAGTGTTTGTGAT+TGG	0.357570	4.3:-28871401	None:intergenic
AGACACCTTTGTCTTCGTTT+TGG	0.361673	4.3:-28871462	None:intergenic
GCTTCAGACCCGACCCGAAC+TGG	0.379399	4.3:-28871808	None:intergenic
TCTGAAGCTGCCCCGTTTCG+CGG	0.386385	4.3:+28871823	MS.gene96823:CDS
GCAGGTGCAGCAGTTGATGC+GGG	0.394326	4.3:-28871251	None:intergenic
GGCTTGGTAATGCTCCAGTT+CGG	0.396045	4.3:+28871794	MS.gene96823:CDS
GCTTGGTAATGCTCCAGTTC+GGG	0.402093	4.3:+28871795	MS.gene96823:CDS
GTGGAACTTAATGTCAGTTC+AGG	0.409244	4.3:+28871745	MS.gene96823:CDS
ACTAAACGACACCGTTTGTC+TGG	0.423254	4.3:+28871712	MS.gene96823:CDS
TCTGCCGAGGCACGTGGTGG+TGG	0.430137	4.3:+28871607	MS.gene96823:CDS
TTGATGCGGGGTTCCTGCGC+GGG	0.451168	4.3:-28871238	None:intergenic
CTGAAGCTGCCCCGTTTCGC+GGG	0.451197	4.3:+28871824	MS.gene96823:CDS
CACTCCACCACCACGTGCCT+CGG	0.465177	4.3:-28871611	None:intergenic
GCAACCCAGAACCCGCGAAA+CGG	0.474820	4.3:-28871835	None:intergenic
GTAATGCTCCAGTTCGGGTC+GGG	0.485181	4.3:+28871800	MS.gene96823:CDS
GAGTTCGGAGTGTGAGCCAC+AGG	0.493976	4.3:-28871315	None:intergenic
AGGTGAATCCTAAAGGGATC+AGG	0.497883	4.3:+28871773	MS.gene96823:CDS
GGTCAGAAGGTGAATCCTAA+AGG	0.505752	4.3:+28871766	MS.gene96823:CDS
GTCAGAAGGTGAATCCTAAA+GGG	0.505982	4.3:+28871767	MS.gene96823:CDS
AGCAGGTGCAGCAGTTGATG+CGG	0.513768	4.3:-28871252	None:intergenic
CGTCAACACCACGCGTCCAC+TGG	0.520956	4.3:+28871525	MS.gene96823:CDS
GGTGATGAAGCTAGTGCAGC+AGG	0.524004	4.3:-28871269	None:intergenic
GGTAATGCTCCAGTTCGGGT+CGG	0.527404	4.3:+28871799	MS.gene96823:CDS
AACGACACCGTTTGTCTGGA+GGG	0.528137	4.3:+28871716	MS.gene96823:CDS
GTAGATGGCGCGGCCCGCGC+AGG	0.530494	4.3:+28871225	None:intergenic
GGAGTGTGAGCCACAGGAAG+AGG	0.532200	4.3:-28871309	None:intergenic
GAGGATGGTGAGGCCTGTGC+TGG	0.544681	4.3:-28871290	None:intergenic
CATTTGCCCCTCCAGACAAA+CGG	0.547066	4.3:-28871723	None:intergenic
GAAACCATCGAGAGTGGAGT+TGG	0.554819	4.3:-28871548	None:intergenic
GCCTCACCATCCTCTTCCTG+TGG	0.560087	4.3:+28871299	MS.gene96823:CDS
TAATGTCAGTTCAGGTCAGA+AGG	0.561149	4.3:+28871753	MS.gene96823:CDS
AACCCAGAACCCGCGAAACG+GGG	0.561738	4.3:-28871833	None:intergenic
TTTGTCTGGAGGGGCAAATG+TGG	0.563437	4.3:+28871726	MS.gene96823:CDS
AATCCTAAAGGGATCAGGCT+TGG	0.567380	4.3:+28871778	MS.gene96823:CDS
AAACACTTCCTTGCACTGTA+CGG	0.569559	4.3:-28871636	None:intergenic
TGGGGTTCTGCCGAGGCACG+TGG	0.580720	4.3:+28871601	MS.gene96823:CDS
GGAGTGAACCGTACAGTGCA+AGG	0.581559	4.3:+28871628	MS.gene96823:CDS
ACTAGCTTCATCACCAGCAC+AGG	0.582415	4.3:+28871277	MS.gene96823:CDS
TGAGAAATGGGGTTCTGCCG+AGG	0.598645	4.3:+28871594	MS.gene96823:CDS
CAGGTGCAGCAGTTGATGCG+GGG	0.599703	4.3:-28871250	None:intergenic
TTGGTAGAAACCATCGAGAG+TGG	0.602681	4.3:-28871554	None:intergenic
CTGGCCAACTCCACTCTCGA+TGG	0.603518	4.3:+28871544	MS.gene96823:CDS
GACGCGTGGTGTTGACGTCG+AGG	0.608775	4.3:-28871519	None:intergenic
AAACGACACCGTTTGTCTGG+AGG	0.617610	4.3:+28871715	MS.gene96823:CDS
GGAGTTGGCCAGTGGACGCG+TGG	0.622642	4.3:-28871533	None:intergenic
GGTTCTGCCGAGGCACGTGG+TGG	0.625771	4.3:+28871604	MS.gene96823:CDS
TCGAGAGTGGAGTTGGCCAG+TGG	0.626154	4.3:-28871541	None:intergenic
GAGAGCAAATGTAAGACGAA+TGG	0.627099	4.3:-28871494	None:intergenic
TGTGAGCCACAGGAAGAGGA+TGG	0.629132	4.3:-28871305	None:intergenic
GTTGATGCGGGGTTCCTGCG+CGG	0.633498	4.3:-28871239	None:intergenic
ATCAACCACCGTAGATGGCG+CGG	0.634709	4.3:+28871215	None:intergenic
GCCACAGGAAGAGGATGGTG+AGG	0.648443	4.3:-28871300	None:intergenic
ACGACACCGTTTGTCTGGAG+GGG	0.667289	4.3:+28871717	MS.gene96823:CDS
ACTCTCGATGGTTTCTACCA+AGG	0.673268	4.3:+28871556	MS.gene96823:CDS
GATGGTTTCTACCAAGGACA+TGG	0.676986	4.3:+28871562	MS.gene96823:CDS
GCAAACCAAAACGAAGACAA+AGG	0.684119	4.3:+28871457	MS.gene96823:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTGATTGAATGAAGCATTT+TGG	-	chr4.3:28871346-28871365	None:intergenic	30.0%
	ATGGTAAAAACGCTGAGAAA+TGG	+	chr4.3:28871581-28871600	MS.gene96823:CDS	35.0%
	TGGTAAAAACGCTGAGAAAT+GGG	+	chr4.3:28871582-28871601	MS.gene96823:CDS	35.0%
!	GTTGTTAGAGTGTTTGTGAT+TGG	-	chr4.3:28871404-28871423	None:intergenic	35.0%
!!	TGGAACTTTTGTTTCTCTCT+TGG	+	chr4.3:28871864-28871883	MS.gene96823:CDS	35.0%
!!!	AGCATTTTGGTTGTTGAGTT+CGG	-	chr4.3:28871333-28871352	None:intergenic	35.0%
!!!	GCAAGGAAGTGTTTTCTTAA+GGG	+	chr4.3:28871645-28871664	MS.gene96823:CDS	35.0%
!!!	TGCAAGGAAGTGTTTTCTTA+AGG	+	chr4.3:28871644-28871663	MS.gene96823:CDS	35.0%
	AAACACTTCCTTGCACTGTA+CGG	-	chr4.3:28871639-28871658	None:intergenic	40.0%
	GAGAGCAAATGTAAGACGAA+TGG	-	chr4.3:28871497-28871516	None:intergenic	40.0%
	GCAAACCAAAACGAAGACAA+AGG	+	chr4.3:28871457-28871476	MS.gene96823:CDS	40.0%
	GGTAAAAACGCTGAGAAATG+GGG	+	chr4.3:28871583-28871602	MS.gene96823:CDS	40.0%
	GTCAGAAGGTGAATCCTAAA+GGG	+	chr4.3:28871767-28871786	MS.gene96823:CDS	40.0%
	GTGGAACTTAATGTCAGTTC+AGG	+	chr4.3:28871745-28871764	MS.gene96823:CDS	40.0%
	TAATGTCAGTTCAGGTCAGA+AGG	+	chr4.3:28871753-28871772	MS.gene96823:CDS	40.0%
!	AGACACCTTTGTCTTCGTTT+TGG	-	chr4.3:28871465-28871484	None:intergenic	40.0%
!!	GTGAGTGTTTTGTTGAGAGA+AGG	-	chr4.3:28871376-28871395	None:intergenic	40.0%
!!	GTGTTTTGTTGAGAGAAGGA+AGG	-	chr4.3:28871372-28871391	None:intergenic	40.0%
!!!	AGGAAGTGTTTTCTTAAGGG+TGG	+	chr4.3:28871648-28871667	MS.gene96823:CDS	40.0%
!!!	CGTTTTGGTTTGCTAGTTTG+AGG	-	chr4.3:28871450-28871469	None:intergenic	40.0%
	AATCCTAAAGGGATCAGGCT+TGG	+	chr4.3:28871778-28871797	MS.gene96823:CDS	45.0%
	ACTAAACGACACCGTTTGTC+TGG	+	chr4.3:28871712-28871731	MS.gene96823:CDS	45.0%
	ACTCTCGATGGTTTCTACCA+AGG	+	chr4.3:28871556-28871575	MS.gene96823:CDS	45.0%
	AGGTGAATCCTAAAGGGATC+AGG	+	chr4.3:28871773-28871792	MS.gene96823:CDS	45.0%
	GATGGTTTCTACCAAGGACA+TGG	+	chr4.3:28871562-28871581	MS.gene96823:CDS	45.0%
	GGTCAGAAGGTGAATCCTAA+AGG	+	chr4.3:28871766-28871785	MS.gene96823:CDS	45.0%
	TTACCAAGCCTGATCCCTTT+AGG	-	chr4.3:28871784-28871803	None:intergenic	45.0%
	TTGGTAGAAACCATCGAGAG+TGG	-	chr4.3:28871557-28871576	None:intergenic	45.0%
!	CAGCGTTTTTACCATGTCCT+TGG	-	chr4.3:28871576-28871595	None:intergenic	45.0%
	AAACGACACCGTTTGTCTGG+AGG	+	chr4.3:28871715-28871734	MS.gene96823:CDS	50.0%
	AACGACACCGTTTGTCTGGA+GGG	+	chr4.3:28871716-28871735	MS.gene96823:CDS	50.0%
	CATTTGCCCCTCCAGACAAA+CGG	-	chr4.3:28871726-28871745	None:intergenic	50.0%
	GAAACCATCGAGAGTGGAGT+TGG	-	chr4.3:28871551-28871570	None:intergenic	50.0%
	TTTGTCTGGAGGGGCAAATG+TGG	+	chr4.3:28871726-28871745	MS.gene96823:CDS	50.0%
!	ACTAGCTTCATCACCAGCAC+AGG	+	chr4.3:28871277-28871296	MS.gene96823:CDS	50.0%
!!	GCTTGGTAATGCTCCAGTTC+GGG	+	chr4.3:28871795-28871814	MS.gene96823:CDS	50.0%
!!	GGCTTGGTAATGCTCCAGTT+CGG	+	chr4.3:28871794-28871813	MS.gene96823:CDS	50.0%
	ACGACACCGTTTGTCTGGAG+GGG	+	chr4.3:28871717-28871736	MS.gene96823:CDS	55.0%
	GGAGTGAACCGTACAGTGCA+AGG	+	chr4.3:28871628-28871647	MS.gene96823:CDS	55.0%
	GGTAATGCTCCAGTTCGGGT+CGG	+	chr4.3:28871799-28871818	MS.gene96823:CDS	55.0%
	GGTGATGAAGCTAGTGCAGC+AGG	-	chr4.3:28871272-28871291	None:intergenic	55.0%
	GTAATGCTCCAGTTCGGGTC+GGG	+	chr4.3:28871800-28871819	MS.gene96823:CDS	55.0%
	TGAGAAATGGGGTTCTGCCG+AGG	+	chr4.3:28871594-28871613	MS.gene96823:CDS	55.0%
	TGTGAGCCACAGGAAGAGGA+TGG	-	chr4.3:28871308-28871327	None:intergenic	55.0%
!	AGCAGGTGCAGCAGTTGATG+CGG	-	chr4.3:28871255-28871274	None:intergenic	55.0%
!!	GGGTTCTGGGTTGCTCTTTC+TGG	+	chr4.3:28871844-28871863	MS.gene96823:CDS	55.0%
	AACCCAGAACCCGCGAAACG+GGG	-	chr4.3:28871836-28871855	None:intergenic	60.0%
	CAACCCAGAACCCGCGAAAC+GGG	-	chr4.3:28871837-28871856	None:intergenic	60.0%
	CTGGCCAACTCCACTCTCGA+TGG	+	chr4.3:28871544-28871563	MS.gene96823:CDS	60.0%
	GAGTTCGGAGTGTGAGCCAC+AGG	-	chr4.3:28871318-28871337	None:intergenic	60.0%
	GCAACCCAGAACCCGCGAAA+CGG	-	chr4.3:28871838-28871857	None:intergenic	60.0%
	GCCACAGGAAGAGGATGGTG+AGG	-	chr4.3:28871303-28871322	None:intergenic	60.0%
	GCCTCACCATCCTCTTCCTG+TGG	+	chr4.3:28871299-28871318	MS.gene96823:CDS	60.0%
	GGAGTGTGAGCCACAGGAAG+AGG	-	chr4.3:28871312-28871331	None:intergenic	60.0%
	TCGAGAGTGGAGTTGGCCAG+TGG	-	chr4.3:28871544-28871563	None:intergenic	60.0%
!	CAGGTGCAGCAGTTGATGCG+GGG	-	chr4.3:28871253-28871272	None:intergenic	60.0%
!	GCAGGTGCAGCAGTTGATGC+GGG	-	chr4.3:28871254-28871273	None:intergenic	60.0%
!	TCTGAAGCTGCCCCGTTTCG+CGG	+	chr4.3:28871823-28871842	MS.gene96823:CDS	60.0%
	CACTCCACCACCACGTGCCT+CGG	-	chr4.3:28871614-28871633	None:intergenic	65.0%
	CGTCAACACCACGCGTCCAC+TGG	+	chr4.3:28871525-28871544	MS.gene96823:CDS	65.0%
	CTGAAGCTGCCCCGTTTCGC+GGG	+	chr4.3:28871824-28871843	MS.gene96823:CDS	65.0%
	GAGGATGGTGAGGCCTGTGC+TGG	-	chr4.3:28871293-28871312	None:intergenic	65.0%
	GCTTCAGACCCGACCCGAAC+TGG	-	chr4.3:28871811-28871830	None:intergenic	65.0%
	GTTGATGCGGGGTTCCTGCG+CGG	-	chr4.3:28871242-28871261	None:intergenic	65.0%
	TGCCCCGTTTCGCGGGTTCT+GGG	+	chr4.3:28871831-28871850	MS.gene96823:CDS	65.0%
	TTGATGCGGGGTTCCTGCGC+GGG	-	chr4.3:28871241-28871260	None:intergenic	65.0%
!!	GACGCGTGGTGTTGACGTCG+AGG	-	chr4.3:28871522-28871541	None:intergenic	65.0%
	CTGCCCCGTTTCGCGGGTTC+TGG	+	chr4.3:28871830-28871849	MS.gene96823:CDS	70.0%
	GGAGTTGGCCAGTGGACGCG+TGG	-	chr4.3:28871536-28871555	None:intergenic	70.0%
	GGTTCTGCCGAGGCACGTGG+TGG	+	chr4.3:28871604-28871623	MS.gene96823:CDS	70.0%
	TCTGCCGAGGCACGTGGTGG+TGG	+	chr4.3:28871607-28871626	MS.gene96823:CDS	70.0%
	TGGGGTTCTGCCGAGGCACG+TGG	+	chr4.3:28871601-28871620	MS.gene96823:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	28871229	28871906	28871229	ID=MS.gene96823
chr4.3	mRNA	28871229	28871906	28871229	ID=MS.gene96823.t1;Parent=MS.gene96823
chr4.3	exon	28871229	28871906	28871229	ID=MS.gene96823.t1.exon1;Parent=MS.gene96823.t1
chr4.3	CDS	28871229	28871906	28871229	ID=cds.MS.gene96823.t1;Parent=MS.gene96823.t1

Gene Sequence

>MS.gene96823

ATGGCGCGGCCCGCGCAGGAACCCCGCATCAACTGCTGCACCTGCTGCACTAGCTTCATCACCAGCACAGGCCTCACCATCCTCTTCCTGTGGCTCACACTCCGAACTCAACAACCAAAATGCTTCATTCAATCACTCTACCTTCCTTCTCTCAACAAAACACTCACTTCAACCAATCACAAACACTCTAACAACAACAACAACACTATCATCTTCAACCTCAAACTAGCAAACCAAAACGAAGACAAAGGTGTCTACTATGACGCCATTCGTCTTACATTTGCTCTCTTCCTCGACGTCAACACCACGCGTCCACTGGCCAACTCCACTCTCGATGGTTTCTACCAAGGACATGGTAAAAACGCTGAGAAATGGGGTTCTGCCGAGGCACGTGGTGGTGGAGTGAACCGTACAGTGCAAGGAAGTGTTTTCTTAAGGGTGGAGTTTGTCACACGCGTTAAGTATAAGATTCTGTTTGTATATACTAAACGACACCGTTTGTCTGGAGGGGCAAATGTGGAACTTAATGTCAGTTCAGGTCAGAAGGTGAATCCTAAAGGGATCAGGCTTGGTAATGCTCCAGTTCGGGTCGGGTCTGAAGCTGCCCCGTTTCGCGGGTTCTGGGTTGCTCTTTCTGGAACTTTTGTTTCTCTCTTGGTTCTTAGTGCTTTTACTTGA

Protein sequence

>MS.gene96823.t1

MARPAQEPRINCCTCCTSFITSTGLTILFLWLTLRTQQPKCFIQSLYLPSLNKTLTSTNHKHSNNNNNTIIFNLKLANQNEDKGVYYDAIRLTFALFLDVNTTRPLANSTLDGFYQGHGKNAEKWGSAEARGGGVNRTVQGSVFLRVEFVTRVKYKILFVYTKRHRLSGGANVELNVSSGQKVNPKGIRLGNAPVRVGSEAAPFRGFWVALSGTFVSLLVLSAFT