AlfalfaGEDB | MS.gene69812

M. sativa cultivar XinJiangDaYe / MS.gene69812

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69812.t1	XP_003596635.1	93.6	219	10	1	1	219	1	215	4.60E-111	410.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69812.t1	O48915	45.6	182	92	3	8	185	13	191	5.5e-36	152.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69812.t1	G7IJL6	93.6	219	10	1	1	219	1	215	3.4e-111	410.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene69812	0.83169	1.45E-55	-1.69E-46
MS.gene04917	MS.gene69812	0.828731	7.64E-55	-1.69E-46
MS.gene04998	MS.gene69812	0.819988	8.70E-53	-1.69E-46
MS.gene058526	MS.gene69812	0.813146	2.97E-51	-1.69E-46
MS.gene058527	MS.gene69812	0.81292	3.33E-51	-1.69E-46
MS.gene058528	MS.gene69812	0.811992	5.30E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene69812	MS.gene87282	PPI
MS.gene69812	MS.gene006756	PPI
MS.gene96823	MS.gene69812	PPI
MS.gene69812	MS.gene99821	PPI
MS.gene031547	MS.gene69812	PPI
MS.gene69812	MS.gene031261	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69812.t1	MTR_2g082990	93.182	220	11	1	1	220	1	216	1.23e-148	412
MS.gene69812.t1	MTR_4g081200	46.698	212	102	5	8	210	11	220	2.70e-58	184

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69812.t1	AT3G20600	41.905	210	114	5	8	213	13	218	4.83e-47	155
MS.gene69812.t1	AT3G20590	42.857	182	96	4	9	185	14	192	1.56e-34	124
MS.gene69812.t1	AT3G20610	35.323	201	116	7	22	215	26	219	3.12e-29	109
MS.gene69812.t1	AT4G05220	22.159	176	122	4	13	180	47	215	2.42e-11	61.6

Find 59 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATACCTTTATCTTTATT+AGG	0.156001	2.4:-16423281	None:intergenic
TGCGGTTAAGTATAAGATTT+TGG	0.193625	2.4:+16423511	MS.gene69812:CDS
TGGAGATTCTGGGGAGAAAA+TGG	0.196345	2.4:+16423583	MS.gene69812:CDS
CTTGGTAATGCTACTGTAAA+TGG	0.197197	2.4:+16423362	MS.gene69812:CDS
CATCATAACCATAGGCTTAA+TGG	0.228595	2.4:+16423118	MS.gene69812:CDS
TGATCTTGCTGTTGTGTTTA+GGG	0.236469	2.4:-16423222	None:intergenic
AATGTGGAAATTGGAGATTC+TGG	0.246829	2.4:+16423572	MS.gene69812:CDS
GAATACCTTTATCTTTATTA+GGG	0.259706	2.4:-16423280	None:intergenic
TTGATCTTGCTGTTGTGTTT+AGG	0.268959	2.4:-16423223	None:intergenic
ATCGTGCGCTTCTTGTGTTT+TGG	0.270001	2.4:+16423681	MS.gene69812:CDS
TTGATGGTAAAGTTTATCTA+AGG	0.284290	2.4:+16423474	MS.gene69812:CDS
GGAGGGGCAAATGTGGAAAT+TGG	0.299189	2.4:+16423563	MS.gene69812:CDS
AAGGTGAAGTTAAGATGAAT+AGG	0.325470	2.4:-16423311	None:intergenic
GTCTTGTTTATCATTGGTCT+TGG	0.334988	2.4:+16423704	MS.gene69812:CDS
AGCTGTTGTTCATGCTGTTC+AGG	0.351132	2.4:+16423092	MS.gene69812:CDS
AATTTAACTGCGAGTGTTAA+GGG	0.363654	2.4:+16423446	MS.gene69812:CDS
GTATGCTTAGCCATATGAAT+AGG	0.380130	2.4:-16423142	None:intergenic
GTTAACCCTAATAAAGATAA+AGG	0.389560	2.4:+16423275	MS.gene69812:CDS
GAATTTAACTGCGAGTGTTA+AGG	0.395995	2.4:+16423445	MS.gene69812:CDS
AGTGTTGAGAGTATTGTAAA+AGG	0.426836	2.4:-16423330	None:intergenic
ACTGTAAATGGATTCTATCA+AGG	0.430123	2.4:+16423374	MS.gene69812:CDS
GGAGATTCTGGGGAGAAAAT+GGG	0.447531	2.4:+16423584	MS.gene69812:CDS
AAAGAGATCGGGTATGGGGA+GGG	0.451284	2.4:+16423546	MS.gene69812:CDS
AGTGTTAAGGGTGTTGTTGA+TGG	0.454047	2.4:+16423458	MS.gene69812:CDS
GCTTAATGGCCCTATTCATA+TGG	0.461217	2.4:+16423132	MS.gene69812:CDS
ATGTGGAAATTGGAGATTCT+GGG	0.462099	2.4:+16423573	MS.gene69812:CDS
AGGTGAAGTTAAGATGAATA+GGG	0.473108	2.4:-16423310	None:intergenic
GGTATGGGGAGGGGCAAATG+TGG	0.473413	2.4:+16423556	MS.gene69812:CDS
TATGCTTAGCCATATGAATA+GGG	0.474917	2.4:-16423141	None:intergenic
AAGAAAAGTGTGAGGCTTGG+AGG	0.475890	2.4:+16423608	MS.gene69812:CDS
CAGCATGAACAACAGCTGTT+GGG	0.497130	2.4:-16423086	None:intergenic
GAATAGGGCCATTAAGCCTA+TGG	0.501191	2.4:-16423126	None:intergenic
ATACGAAAAGAGATCGGGTA+TGG	0.508627	2.4:+16423540	MS.gene69812:CDS
TCCACACACTTTCCATGCGC+CGG	0.510157	2.4:-16423654	None:intergenic
GAACCAAGGGTGATAGTGTC+CGG	0.513905	2.4:+16423635	MS.gene69812:CDS
AAGAGATCGGGTATGGGGAG+GGG	0.523912	2.4:+16423547	MS.gene69812:CDS
AAGAATGGAGTGGTGGAAGC+TGG	0.525007	2.4:+16423416	MS.gene69812:CDS
AGAAAAGTGTGAGGCTTGGA+GGG	0.526565	2.4:+16423609	MS.gene69812:CDS
TCAGGTTTCATCATAACCAT+AGG	0.529456	2.4:+16423110	MS.gene69812:CDS
CTCAACACTACTCTTCCACT+TGG	0.532729	2.4:+16423344	MS.gene69812:CDS
GGGTGATAGTGTCCGGCGCA+TGG	0.534538	2.4:+16423642	MS.gene69812:CDS
TACGAAAAGAGATCGGGTAT+GGG	0.536489	2.4:+16423541	MS.gene69812:CDS
ACAGCATGAACAACAGCTGT+TGG	0.541536	2.4:-16423087	None:intergenic
GGAAAGAAAAGTGTGAGGCT+TGG	0.543228	2.4:+16423605	MS.gene69812:CDS
TTGGTATACGAAAAGAGATC+GGG	0.544628	2.4:+16423535	MS.gene69812:CDS
AAAAGAGATCGGGTATGGGG+AGG	0.556071	2.4:+16423545	MS.gene69812:CDS
GCGCCGGACACTATCACCCT+TGG	0.558967	2.4:-16423638	None:intergenic
CTGTAAATGGATTCTATCAA+GGG	0.559809	2.4:+16423375	MS.gene69812:CDS
TTTGGTATACGAAAAGAGAT+CGG	0.560749	2.4:+16423534	MS.gene69812:CDS
TGGTAAAGTTTATCTAAGGG+TGG	0.560905	2.4:+16423478	MS.gene69812:CDS
TGATGGTAAAGTTTATCTAA+GGG	0.567329	2.4:+16423475	MS.gene69812:CDS
ACGAAAAGAGATCGGGTATG+GGG	0.597221	2.4:+16423542	MS.gene69812:CDS
GGCTTGGAGGGAATGAACCA+AGG	0.634018	2.4:+16423621	MS.gene69812:CDS
GCTTGGAGGGAATGAACCAA+GGG	0.643282	2.4:+16423622	MS.gene69812:CDS
TGTGGAAATTGGAGATTCTG+GGG	0.648415	2.4:+16423574	MS.gene69812:CDS
GGCGCATGGAAAGTGTGTGG+AGG	0.656040	2.4:+16423656	MS.gene69812:CDS
TCCGGCGCATGGAAAGTGTG+TGG	0.671773	2.4:+16423653	MS.gene69812:CDS
TTTACAGTAGCATTACCAAG+TGG	0.674013	2.4:-16423359	None:intergenic
AAATGGGAAAGAAAAGTGTG+AGG	0.693699	2.4:+16423600	MS.gene69812:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAATACCTTTATCTTTATTA+GGG	-	chr2.4:16423283-16423302	None:intergenic	20.0%
!!	TGAATACCTTTATCTTTATT+AGG	-	chr2.4:16423284-16423303	None:intergenic	20.0%
!!!	TTAAGTATAAGATTTTGGTT+TGG	+	chr2.4:16423516-16423535	MS.gene69812:CDS	20.0%
!	GAGAAAAAAGCTAAAAAGAA+TGG	+	chr2.4:16423401-16423420	MS.gene69812:CDS	25.0%
!	GTTAACCCTAATAAAGATAA+AGG	+	chr2.4:16423275-16423294	MS.gene69812:CDS	25.0%
!	TGATGGTAAAGTTTATCTAA+GGG	+	chr2.4:16423475-16423494	MS.gene69812:CDS	25.0%
!!	ATTTTGTCTATGTAAAGCTT+TGG	-	chr2.4:16423179-16423198	None:intergenic	25.0%
!!	TTGATGGTAAAGTTTATCTA+AGG	+	chr2.4:16423474-16423493	MS.gene69812:CDS	25.0%
	AAAAGCTAAAAAGAATGGAG+TGG	+	chr2.4:16423406-16423425	MS.gene69812:CDS	30.0%
	AAGGTGAAGTTAAGATGAAT+AGG	-	chr2.4:16423314-16423333	None:intergenic	30.0%
	AATTTAACTGCGAGTGTTAA+GGG	+	chr2.4:16423446-16423465	MS.gene69812:CDS	30.0%
	AGGTGAAGTTAAGATGAATA+GGG	-	chr2.4:16423313-16423332	None:intergenic	30.0%
	TATGCTTAGCCATATGAATA+GGG	-	chr2.4:16423144-16423163	None:intergenic	30.0%
	TTTGGTATACGAAAAGAGAT+CGG	+	chr2.4:16423534-16423553	MS.gene69812:CDS	30.0%
!	ACTGTAAATGGATTCTATCA+AGG	+	chr2.4:16423374-16423393	MS.gene69812:CDS	30.0%
!	CTGTAAATGGATTCTATCAA+GGG	+	chr2.4:16423375-16423394	MS.gene69812:CDS	30.0%
!	TGCGGTTAAGTATAAGATTT+TGG	+	chr2.4:16423511-16423530	MS.gene69812:CDS	30.0%
!!	AGTGTTGAGAGTATTGTAAA+AGG	-	chr2.4:16423333-16423352	None:intergenic	30.0%
!!	GTTAACAAGTTTTAACGTGA+AGG	-	chr2.4:16423261-16423280	None:intergenic	30.0%
!!!	TTTTGGGTCTTGTTTATCAT+TGG	+	chr2.4:16423698-16423717	MS.gene69812:CDS	30.0%
	AATGTGGAAATTGGAGATTC+TGG	+	chr2.4:16423572-16423591	MS.gene69812:CDS	35.0%
	ATGTGGAAATTGGAGATTCT+GGG	+	chr2.4:16423573-16423592	MS.gene69812:CDS	35.0%
	CATCATAACCATAGGCTTAA+TGG	+	chr2.4:16423118-16423137	MS.gene69812:CDS	35.0%
	GAATTTAACTGCGAGTGTTA+AGG	+	chr2.4:16423445-16423464	MS.gene69812:CDS	35.0%
	GTATGCTTAGCCATATGAAT+AGG	-	chr2.4:16423145-16423164	None:intergenic	35.0%
	TCAGGTTTCATCATAACCAT+AGG	+	chr2.4:16423110-16423129	MS.gene69812:CDS	35.0%
	TGGTAAAGTTTATCTAAGGG+TGG	+	chr2.4:16423478-16423497	MS.gene69812:CDS	35.0%
	TTGGTATACGAAAAGAGATC+GGG	+	chr2.4:16423535-16423554	MS.gene69812:CDS	35.0%
	TTTACAGTAGCATTACCAAG+TGG	-	chr2.4:16423362-16423381	None:intergenic	35.0%
!	AAATGGGAAAGAAAAGTGTG+AGG	+	chr2.4:16423600-16423619	MS.gene69812:CDS	35.0%
!	TTTTAACGTGAAGGTGATAG+TGG	-	chr2.4:16423252-16423271	None:intergenic	35.0%
!!	CTTGGTAATGCTACTGTAAA+TGG	+	chr2.4:16423362-16423381	MS.gene69812:CDS	35.0%
!!	GTCTTGTTTATCATTGGTCT+TGG	+	chr2.4:16423704-16423723	MS.gene69812:CDS	35.0%
!!	TGATCTTGCTGTTGTGTTTA+GGG	-	chr2.4:16423225-16423244	None:intergenic	35.0%
!!	TTGATCTTGCTGTTGTGTTT+AGG	-	chr2.4:16423226-16423245	None:intergenic	35.0%
!!!	AAGGGTGGATTATTTTACTG+CGG	+	chr2.4:16423493-16423512	MS.gene69812:CDS	35.0%
!!!	TGTGTTTAGGGTTTTGTTGA+GGG	-	chr2.4:16423213-16423232	None:intergenic	35.0%
!!!	TTGTGTTTAGGGTTTTGTTG+AGG	-	chr2.4:16423214-16423233	None:intergenic	35.0%
	AGCTAAAAAGAATGGAGTGG+TGG	+	chr2.4:16423409-16423428	MS.gene69812:CDS	40.0%
	ATACGAAAAGAGATCGGGTA+TGG	+	chr2.4:16423540-16423559	MS.gene69812:CDS	40.0%
	GCTTAATGGCCCTATTCATA+TGG	+	chr2.4:16423132-16423151	MS.gene69812:CDS	40.0%
	TACGAAAAGAGATCGGGTAT+GGG	+	chr2.4:16423541-16423560	MS.gene69812:CDS	40.0%
	TGTGGAAATTGGAGATTCTG+GGG	+	chr2.4:16423574-16423593	MS.gene69812:CDS	40.0%
!!	AGTGTTAAGGGTGTTGTTGA+TGG	+	chr2.4:16423458-16423477	MS.gene69812:CDS	40.0%
	ACAGCATGAACAACAGCTGT+TGG	-	chr2.4:16423090-16423109	None:intergenic	45.0%
	ACGAAAAGAGATCGGGTATG+GGG	+	chr2.4:16423542-16423561	MS.gene69812:CDS	45.0%
	AGCTGTTGTTCATGCTGTTC+AGG	+	chr2.4:16423092-16423111	MS.gene69812:CDS	45.0%
	CAGCATGAACAACAGCTGTT+GGG	-	chr2.4:16423089-16423108	None:intergenic	45.0%
	CTCAACACTACTCTTCCACT+TGG	+	chr2.4:16423344-16423363	MS.gene69812:CDS	45.0%
	GAATAGGGCCATTAAGCCTA+TGG	-	chr2.4:16423129-16423148	None:intergenic	45.0%
!	AAGAAAAGTGTGAGGCTTGG+AGG	+	chr2.4:16423608-16423627	MS.gene69812:CDS	45.0%
!	AGAAAAGTGTGAGGCTTGGA+GGG	+	chr2.4:16423609-16423628	MS.gene69812:CDS	45.0%
!	ATCGTGCGCTTCTTGTGTTT+TGG	+	chr2.4:16423681-16423700	MS.gene69812:CDS	45.0%
!	GGAAAGAAAAGTGTGAGGCT+TGG	+	chr2.4:16423605-16423624	MS.gene69812:CDS	45.0%
!	GGAGATTCTGGGGAGAAAAT+GGG	+	chr2.4:16423584-16423603	MS.gene69812:CDS	45.0%
!	TCGTGCGCTTCTTGTGTTTT+GGG	+	chr2.4:16423682-16423701	MS.gene69812:CDS	45.0%
!	TGGAGATTCTGGGGAGAAAA+TGG	+	chr2.4:16423583-16423602	MS.gene69812:CDS	45.0%
!!!	TTTAGGGTTTTGTTGAGGGC+AGG	-	chr2.4:16423209-16423228	None:intergenic	45.0%
	AAAAGAGATCGGGTATGGGG+AGG	+	chr2.4:16423545-16423564	MS.gene69812:CDS	50.0%
	AAAGAGATCGGGTATGGGGA+GGG	+	chr2.4:16423546-16423565	MS.gene69812:CDS	50.0%
	GCTTGGAGGGAATGAACCAA+GGG	+	chr2.4:16423622-16423641	MS.gene69812:CDS	50.0%
	GGAGGGGCAAATGTGGAAAT+TGG	+	chr2.4:16423563-16423582	MS.gene69812:CDS	50.0%
!	AAGAATGGAGTGGTGGAAGC+TGG	+	chr2.4:16423416-16423435	MS.gene69812:CDS	50.0%
!	GAACCAAGGGTGATAGTGTC+CGG	+	chr2.4:16423635-16423654	MS.gene69812:CDS	50.0%
	AAGAGATCGGGTATGGGGAG+GGG	+	chr2.4:16423547-16423566	MS.gene69812:CDS	55.0%
	GGCTTGGAGGGAATGAACCA+AGG	+	chr2.4:16423621-16423640	MS.gene69812:CDS	55.0%
	TCCACACACTTTCCATGCGC+CGG	-	chr2.4:16423657-16423676	None:intergenic	55.0%
!!!	GGGTTTTGTTGAGGGCAGGA+AGG	-	chr2.4:16423205-16423224	None:intergenic	55.0%
	GGTATGGGGAGGGGCAAATG+TGG	+	chr2.4:16423556-16423575	MS.gene69812:CDS	60.0%
!	GGCGCATGGAAAGTGTGTGG+AGG	+	chr2.4:16423656-16423675	MS.gene69812:CDS	60.0%
!	TCCGGCGCATGGAAAGTGTG+TGG	+	chr2.4:16423653-16423672	MS.gene69812:CDS	60.0%
	GCGCCGGACACTATCACCCT+TGG	-	chr2.4:16423641-16423660	None:intergenic	65.0%
!	GGGTGATAGTGTCCGGCGCA+TGG	+	chr2.4:16423642-16423661	MS.gene69812:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	16423071	16423745	16423071	ID=MS.gene69812
chr2.4	mRNA	16423071	16423745	16423071	ID=MS.gene69812.t1;Parent=MS.gene69812
chr2.4	exon	16423071	16423745	16423071	ID=MS.gene69812.t1.exon1;Parent=MS.gene69812.t1
chr2.4	CDS	16423071	16423745	16423071	ID=cds.MS.gene69812.t1;Parent=MS.gene69812.t1

Gene Sequence

>MS.gene69812

ATGGCATCATCAAAACCCAACAGCTGTTGTTCATGCTGTTCAGGTTTCATCATAACCATAGGCTTAATGGCCCTATTCATATGGCTAAGCATACGTGTCGATGAACCAAAGCTTTACATAGACAAAATTTACCTTCCTGCCCTCAACAAAACCCTAAACACAACAGCAAGATCAAACACCACTATCACCTTCACGTTAAAACTTGTTAACCCTAATAAAGATAAAGGTATTCAATATGACCCTATTCATCTTAACTTCACCTTTTACAATACTCTCAACACTACTCTTCCACTTGGTAATGCTACTGTAAATGGATTCTATCAAGGGCATGAGAAAAAAGCTAAAAAGAATGGAGTGGTGGAAGCTGGTGTTAAGAATTTAACTGCGAGTGTTAAGGGTGTTGTTGATGGTAAAGTTTATCTAAGGGTGGATTATTTTACTGCGGTTAAGTATAAGATTTTGGTTTGGTATACGAAAAGAGATCGGGTATGGGGAGGGGCAAATGTGGAAATTGGAGATTCTGGGGAGAAAATGGGAAAGAAAAGTGTGAGGCTTGGAGGGAATGAACCAAGGGTGATAGTGTCCGGCGCATGGAAAGTGTGTGGAGGCTATCGTGCGCTTCTTGTGTTTTGGGTCTTGTTTATCATTGGTCTTGGTTTACTTGACTTTGACTGA

Protein sequence

>MS.gene69812.t1

MASSKPNSCCSCCSGFIITIGLMALFIWLSIRVDEPKLYIDKIYLPALNKTLNTTARSNTTITFTLKLVNPNKDKGIQYDPIHLNFTFYNTLNTTLPLGNATVNGFYQGHEKKAKKNGVVEAGVKNLTASVKGVVDGKVYLRVDYFTAVKYKILVWYTKRDRVWGGANVEIGDSGEKMGKKSVRLGGNEPRVIVSGAWKVCGGYRALLVFWVLFIIGLGLLDFD