AlfalfaGEDB | MS.gene031261

M. sativa cultivar XinJiangDaYe / MS.gene031261

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031261.t1	XP_003596635.1	93.7	221	10	1	1	221	1	217	3.20E-112	414.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031261.t1	O48915	45.6	182	92	3	8	185	13	191	5.4e-36	152.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031261.t1	G7IJL6	93.7	221	10	1	1	221	1	217	2.3e-112	414.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene031261	MS.gene99821	PPI
MS.gene006756	MS.gene031261	PPI
MS.gene96823	MS.gene031261	PPI
MS.gene031261	MS.gene87282	PPI
MS.gene99821	MS.gene031261	PPI
MS.gene87282	MS.gene031261	PPI
MS.gene031547	MS.gene031261	PPI
MS.gene69812	MS.gene031261	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031261.t1	MTR_2g082990	93.665	221	10	1	1	221	1	217	2.88e-150	416
MS.gene031261.t1	MTR_4g081200	46.544	217	110	4	8	221	11	224	9.04e-59	185

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene031261.t1	AT3G20600	41.905	210	114	5	8	213	13	218	3.84e-47	155
MS.gene031261.t1	AT3G20590	42.857	182	96	4	9	185	14	192	1.50e-34	124
MS.gene031261.t1	AT3G20610	35.323	201	116	7	22	215	26	219	3.43e-29	109
MS.gene031261.t1	AT4G05220	22.159	176	122	4	13	180	47	215	1.88e-11	62.0

Find 62 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATACCTTTATCTTTATT+AGG	0.156001	2.1:-18125223	None:intergenic
TGCGGTTAAGTATAAGATTT+TGG	0.193625	2.1:+18125453	MS.gene031261:CDS
TGGAGATTCTGGGGAGAAAA+TGG	0.196345	2.1:+18125525	MS.gene031261:CDS
CTTGGTAATGCTACTGTAAA+TGG	0.197197	2.1:+18125304	MS.gene031261:CDS
CATCATAACCATAGGCTTAA+TGG	0.228595	2.1:+18125060	MS.gene031261:CDS
TGATCTTGCTGTTGTGTTTA+GGG	0.236469	2.1:-18125164	None:intergenic
AATGTGGAAATTGGAGATTC+TGG	0.246829	2.1:+18125514	MS.gene031261:CDS
GAATACCTTTATCTTTATTA+GGG	0.259706	2.1:-18125222	None:intergenic
TTGATCTTGCTGTTGTGTTT+AGG	0.268959	2.1:-18125165	None:intergenic
ATCGTGCGCTTCTTGTGTTT+TGG	0.270001	2.1:+18125623	MS.gene031261:CDS
TTGATGGTAAAGTTTATCTA+AGG	0.284290	2.1:+18125416	MS.gene031261:CDS
GTCTTGTTTATCATTGGTCT+TGG	0.284806	2.1:+18125646	MS.gene031261:CDS
GGAGGGGCAAATGTGGAAAT+TGG	0.299189	2.1:+18125505	MS.gene031261:CDS
AAGGTGAAGTTAAGATGAAT+AGG	0.325470	2.1:-18125253	None:intergenic
AGCTGTTGTTCATGCTGTTC+AGG	0.351132	2.1:+18125034	MS.gene031261:CDS
AATTTAACTGCGAGTGTTAA+GGG	0.363654	2.1:+18125388	MS.gene031261:CDS
GTATGCTTAGCCATATGAAT+AGG	0.380130	2.1:-18125084	None:intergenic
GTTAACCCTAATAAAGATAA+AGG	0.389560	2.1:+18125217	MS.gene031261:CDS
GAATTTAACTGCGAGTGTTA+AGG	0.395995	2.1:+18125387	MS.gene031261:CDS
TCTTGTTTATCATTGGTCTT+GGG	0.420051	2.1:+18125647	MS.gene031261:CDS
AGTGTTGAGAGTATTGTAAA+AGG	0.426836	2.1:-18125272	None:intergenic
ACTGTAAATGGATTCTATCA+AGG	0.430123	2.1:+18125316	MS.gene031261:CDS
GGAGATTCTGGGGAGAAAAT+GGG	0.447531	2.1:+18125526	MS.gene031261:CDS
AAAGAGATCGGGTATGGGGA+GGG	0.451284	2.1:+18125488	MS.gene031261:CDS
AGTGTTAAGGGTGTTGTTGA+TGG	0.454047	2.1:+18125400	MS.gene031261:CDS
GCTTAATGGCCCTATTCATA+TGG	0.461217	2.1:+18125074	MS.gene031261:CDS
ATGTGGAAATTGGAGATTCT+GGG	0.462099	2.1:+18125515	MS.gene031261:CDS
AGGTGAAGTTAAGATGAATA+GGG	0.473108	2.1:-18125252	None:intergenic
GGTATGGGGAGGGGCAAATG+TGG	0.473413	2.1:+18125498	MS.gene031261:CDS
TATGCTTAGCCATATGAATA+GGG	0.474917	2.1:-18125083	None:intergenic
AAGAAAAGTGTGAGGCTTGG+AGG	0.475890	2.1:+18125550	MS.gene031261:CDS
CAGCATGAACAACAGCTGTT+GGG	0.497130	2.1:-18125028	None:intergenic
GAATAGGGCCATTAAGCCTA+TGG	0.501191	2.1:-18125068	None:intergenic
ATACGAAAAGAGATCGGGTA+TGG	0.508627	2.1:+18125482	MS.gene031261:CDS
TCCACACACTTTCCATGCGC+CGG	0.510157	2.1:-18125596	None:intergenic
AAGAATGGAGTGGTGGAAGC+TGG	0.512376	2.1:+18125358	MS.gene031261:CDS
GAACCAAGGGTGATAGTGTC+CGG	0.513905	2.1:+18125577	MS.gene031261:CDS
AAGAGATCGGGTATGGGGAG+GGG	0.523912	2.1:+18125489	MS.gene031261:CDS
AGAAAAGTGTGAGGCTTGGA+GGG	0.526565	2.1:+18125551	MS.gene031261:CDS
TCAGGTTTCATCATAACCAT+AGG	0.529456	2.1:+18125052	MS.gene031261:CDS
CTCAACACTACTCTTCCACT+TGG	0.532729	2.1:+18125286	MS.gene031261:CDS
GGGTGATAGTGTCCGGCGCA+TGG	0.534538	2.1:+18125584	MS.gene031261:CDS
TACGAAAAGAGATCGGGTAT+GGG	0.536489	2.1:+18125483	MS.gene031261:CDS
ACAGCATGAACAACAGCTGT+TGG	0.541536	2.1:-18125029	None:intergenic
GGAAAGAAAAGTGTGAGGCT+TGG	0.543228	2.1:+18125547	MS.gene031261:CDS
TTGGTATACGAAAAGAGATC+GGG	0.544628	2.1:+18125477	MS.gene031261:CDS
AGCTAAGAAGAATGGAGTGG+TGG	0.547361	2.1:+18125351	MS.gene031261:CDS
AAAAGAGATCGGGTATGGGG+AGG	0.556071	2.1:+18125487	MS.gene031261:CDS
GCGCCGGACACTATCACCCT+TGG	0.558967	2.1:-18125580	None:intergenic
CTGTAAATGGATTCTATCAA+GGG	0.559809	2.1:+18125317	MS.gene031261:CDS
TTTGGTATACGAAAAGAGAT+CGG	0.560749	2.1:+18125476	MS.gene031261:CDS
TGGTAAAGTTTATCTAAGGG+TGG	0.560905	2.1:+18125420	MS.gene031261:CDS
TGATGGTAAAGTTTATCTAA+GGG	0.567329	2.1:+18125417	MS.gene031261:CDS
ACGAAAAGAGATCGGGTATG+GGG	0.597221	2.1:+18125484	MS.gene031261:CDS
GGCTTGGAGGGAATGAACCA+AGG	0.634018	2.1:+18125563	MS.gene031261:CDS
GCTTGGAGGGAATGAACCAA+GGG	0.643282	2.1:+18125564	MS.gene031261:CDS
TGTGGAAATTGGAGATTCTG+GGG	0.648415	2.1:+18125516	MS.gene031261:CDS
GGCGCATGGAAAGTGTGTGG+AGG	0.656040	2.1:+18125598	MS.gene031261:CDS
AAAAGCTAAGAAGAATGGAG+TGG	0.661701	2.1:+18125348	MS.gene031261:CDS
TCCGGCGCATGGAAAGTGTG+TGG	0.671773	2.1:+18125595	MS.gene031261:CDS
TTTACAGTAGCATTACCAAG+TGG	0.674013	2.1:-18125301	None:intergenic
AAATGGGAAAGAAAAGTGTG+AGG	0.693699	2.1:+18125542	MS.gene031261:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAATACCTTTATCTTTATTA+GGG	-	chr2.1:18125225-18125244	None:intergenic	20.0%
!!	TGAATACCTTTATCTTTATT+AGG	-	chr2.1:18125226-18125245	None:intergenic	20.0%
!!!	TTAAGTATAAGATTTTGGTT+TGG	+	chr2.1:18125458-18125477	MS.gene031261:CDS	20.0%
!	GTTAACCCTAATAAAGATAA+AGG	+	chr2.1:18125217-18125236	MS.gene031261:CDS	25.0%
!	TGATGGTAAAGTTTATCTAA+GGG	+	chr2.1:18125417-18125436	MS.gene031261:CDS	25.0%
!!	ATTTTGTCTATGTAAAGCTT+TGG	-	chr2.1:18125121-18125140	None:intergenic	25.0%
!!	TTGATGGTAAAGTTTATCTA+AGG	+	chr2.1:18125416-18125435	MS.gene031261:CDS	25.0%
	AAGGTGAAGTTAAGATGAAT+AGG	-	chr2.1:18125256-18125275	None:intergenic	30.0%
	AATTTAACTGCGAGTGTTAA+GGG	+	chr2.1:18125388-18125407	MS.gene031261:CDS	30.0%
	AGGTGAAGTTAAGATGAATA+GGG	-	chr2.1:18125255-18125274	None:intergenic	30.0%
	GAGAAAAAAGCTAAGAAGAA+TGG	+	chr2.1:18125343-18125362	MS.gene031261:CDS	30.0%
	TATGCTTAGCCATATGAATA+GGG	-	chr2.1:18125086-18125105	None:intergenic	30.0%
	TTTGGTATACGAAAAGAGAT+CGG	+	chr2.1:18125476-18125495	MS.gene031261:CDS	30.0%
!	ACTGTAAATGGATTCTATCA+AGG	+	chr2.1:18125316-18125335	MS.gene031261:CDS	30.0%
!	CTGTAAATGGATTCTATCAA+GGG	+	chr2.1:18125317-18125336	MS.gene031261:CDS	30.0%
!	TGCGGTTAAGTATAAGATTT+TGG	+	chr2.1:18125453-18125472	MS.gene031261:CDS	30.0%
!!	AGTGTTGAGAGTATTGTAAA+AGG	-	chr2.1:18125275-18125294	None:intergenic	30.0%
!!	GTTAACAAGTTTTAACGTGA+AGG	-	chr2.1:18125203-18125222	None:intergenic	30.0%
!!	TCTTGTTTATCATTGGTCTT+GGG	+	chr2.1:18125647-18125666	MS.gene031261:CDS	30.0%
!!!	TTTTGGGTCTTGTTTATCAT+TGG	+	chr2.1:18125640-18125659	MS.gene031261:CDS	30.0%
	AAAAGCTAAGAAGAATGGAG+TGG	+	chr2.1:18125348-18125367	MS.gene031261:CDS	35.0%
	AATGTGGAAATTGGAGATTC+TGG	+	chr2.1:18125514-18125533	MS.gene031261:CDS	35.0%
	ATGTGGAAATTGGAGATTCT+GGG	+	chr2.1:18125515-18125534	MS.gene031261:CDS	35.0%
	CATCATAACCATAGGCTTAA+TGG	+	chr2.1:18125060-18125079	MS.gene031261:CDS	35.0%
	GAATTTAACTGCGAGTGTTA+AGG	+	chr2.1:18125387-18125406	MS.gene031261:CDS	35.0%
	GTATGCTTAGCCATATGAAT+AGG	-	chr2.1:18125087-18125106	None:intergenic	35.0%
	TCAGGTTTCATCATAACCAT+AGG	+	chr2.1:18125052-18125071	MS.gene031261:CDS	35.0%
	TGGTAAAGTTTATCTAAGGG+TGG	+	chr2.1:18125420-18125439	MS.gene031261:CDS	35.0%
	TTGGTATACGAAAAGAGATC+GGG	+	chr2.1:18125477-18125496	MS.gene031261:CDS	35.0%
	TTTACAGTAGCATTACCAAG+TGG	-	chr2.1:18125304-18125323	None:intergenic	35.0%
!	AAATGGGAAAGAAAAGTGTG+AGG	+	chr2.1:18125542-18125561	MS.gene031261:CDS	35.0%
!	TTTTAACGTGAAGGTGATAG+TGG	-	chr2.1:18125194-18125213	None:intergenic	35.0%
!!	CTTGGTAATGCTACTGTAAA+TGG	+	chr2.1:18125304-18125323	MS.gene031261:CDS	35.0%
!!	GTCTTGTTTATCATTGGTCT+TGG	+	chr2.1:18125646-18125665	MS.gene031261:CDS	35.0%
!!	TGATCTTGCTGTTGTGTTTA+GGG	-	chr2.1:18125167-18125186	None:intergenic	35.0%
!!	TTGATCTTGCTGTTGTGTTT+AGG	-	chr2.1:18125168-18125187	None:intergenic	35.0%
!!!	AAGGGTGGATTATTTTACTG+CGG	+	chr2.1:18125435-18125454	MS.gene031261:CDS	35.0%
!!!	TGTGTTTAGGGTTTTGTTGA+GGG	-	chr2.1:18125155-18125174	None:intergenic	35.0%
!!!	TTGTGTTTAGGGTTTTGTTG+AGG	-	chr2.1:18125156-18125175	None:intergenic	35.0%
	ATACGAAAAGAGATCGGGTA+TGG	+	chr2.1:18125482-18125501	MS.gene031261:CDS	40.0%
	GCTTAATGGCCCTATTCATA+TGG	+	chr2.1:18125074-18125093	MS.gene031261:CDS	40.0%
	TACGAAAAGAGATCGGGTAT+GGG	+	chr2.1:18125483-18125502	MS.gene031261:CDS	40.0%
	TGTGGAAATTGGAGATTCTG+GGG	+	chr2.1:18125516-18125535	MS.gene031261:CDS	40.0%
!!	AGTGTTAAGGGTGTTGTTGA+TGG	+	chr2.1:18125400-18125419	MS.gene031261:CDS	40.0%
	ACAGCATGAACAACAGCTGT+TGG	-	chr2.1:18125032-18125051	None:intergenic	45.0%
	ACGAAAAGAGATCGGGTATG+GGG	+	chr2.1:18125484-18125503	MS.gene031261:CDS	45.0%
	AGCTAAGAAGAATGGAGTGG+TGG	+	chr2.1:18125351-18125370	MS.gene031261:CDS	45.0%
	AGCTGTTGTTCATGCTGTTC+AGG	+	chr2.1:18125034-18125053	MS.gene031261:CDS	45.0%
	CAGCATGAACAACAGCTGTT+GGG	-	chr2.1:18125031-18125050	None:intergenic	45.0%
	CTCAACACTACTCTTCCACT+TGG	+	chr2.1:18125286-18125305	MS.gene031261:CDS	45.0%
	GAATAGGGCCATTAAGCCTA+TGG	-	chr2.1:18125071-18125090	None:intergenic	45.0%
!	AAGAAAAGTGTGAGGCTTGG+AGG	+	chr2.1:18125550-18125569	MS.gene031261:CDS	45.0%
!	AGAAAAGTGTGAGGCTTGGA+GGG	+	chr2.1:18125551-18125570	MS.gene031261:CDS	45.0%
!	ATCGTGCGCTTCTTGTGTTT+TGG	+	chr2.1:18125623-18125642	MS.gene031261:CDS	45.0%
!	GGAAAGAAAAGTGTGAGGCT+TGG	+	chr2.1:18125547-18125566	MS.gene031261:CDS	45.0%
!	GGAGATTCTGGGGAGAAAAT+GGG	+	chr2.1:18125526-18125545	MS.gene031261:CDS	45.0%
!	TCGTGCGCTTCTTGTGTTTT+GGG	+	chr2.1:18125624-18125643	MS.gene031261:CDS	45.0%
!	TGGAGATTCTGGGGAGAAAA+TGG	+	chr2.1:18125525-18125544	MS.gene031261:CDS	45.0%
!!!	TTTAGGGTTTTGTTGAGGGC+AGG	-	chr2.1:18125151-18125170	None:intergenic	45.0%
	AAAAGAGATCGGGTATGGGG+AGG	+	chr2.1:18125487-18125506	MS.gene031261:CDS	50.0%
	AAAGAGATCGGGTATGGGGA+GGG	+	chr2.1:18125488-18125507	MS.gene031261:CDS	50.0%
	GCTTGGAGGGAATGAACCAA+GGG	+	chr2.1:18125564-18125583	MS.gene031261:CDS	50.0%
	GGAGGGGCAAATGTGGAAAT+TGG	+	chr2.1:18125505-18125524	MS.gene031261:CDS	50.0%
!	AAGAATGGAGTGGTGGAAGC+TGG	+	chr2.1:18125358-18125377	MS.gene031261:CDS	50.0%
!	GAACCAAGGGTGATAGTGTC+CGG	+	chr2.1:18125577-18125596	MS.gene031261:CDS	50.0%
	AAGAGATCGGGTATGGGGAG+GGG	+	chr2.1:18125489-18125508	MS.gene031261:CDS	55.0%
	GGCTTGGAGGGAATGAACCA+AGG	+	chr2.1:18125563-18125582	MS.gene031261:CDS	55.0%
	TCCACACACTTTCCATGCGC+CGG	-	chr2.1:18125599-18125618	None:intergenic	55.0%
!!!	GGGTTTTGTTGAGGGCAGGA+AGG	-	chr2.1:18125147-18125166	None:intergenic	55.0%
	GGTATGGGGAGGGGCAAATG+TGG	+	chr2.1:18125498-18125517	MS.gene031261:CDS	60.0%
!	GGCGCATGGAAAGTGTGTGG+AGG	+	chr2.1:18125598-18125617	MS.gene031261:CDS	60.0%
!	TCCGGCGCATGGAAAGTGTG+TGG	+	chr2.1:18125595-18125614	MS.gene031261:CDS	60.0%
	GCGCCGGACACTATCACCCT+TGG	-	chr2.1:18125583-18125602	None:intergenic	65.0%
!	GGGTGATAGTGTCCGGCGCA+TGG	+	chr2.1:18125584-18125603	MS.gene031261:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	18125013	18125678	18125013	ID=MS.gene031261
chr2.1	mRNA	18125013	18125678	18125013	ID=MS.gene031261.t1;Parent=MS.gene031261
chr2.1	exon	18125013	18125678	18125013	ID=MS.gene031261.t1.exon1;Parent=MS.gene031261.t1
chr2.1	CDS	18125013	18125678	18125013	ID=cds.MS.gene031261.t1;Parent=MS.gene031261.t1

Gene Sequence

>MS.gene031261

ATGGCATCATCAAAACCCAACAGCTGTTGTTCATGCTGTTCAGGTTTCATCATAACCATAGGCTTAATGGCCCTATTCATATGGCTAAGCATACGTGTCGATGAACCAAAGCTTTACATAGACAAAATTTACCTTCCTGCCCTCAACAAAACCCTAAACACAACAGCAAGATCAAACACCACTATCACCTTCACGTTAAAACTTGTTAACCCTAATAAAGATAAAGGTATTCAATATGACCCTATTCATCTTAACTTCACCTTTTACAATACTCTCAACACTACTCTTCCACTTGGTAATGCTACTGTAAATGGATTCTATCAAGGGCATGAGAAAAAAGCTAAGAAGAATGGAGTGGTGGAAGCTGGTGTTAAGAATTTAACTGCGAGTGTTAAGGGTGTTGTTGATGGTAAAGTTTATCTAAGGGTGGATTATTTTACTGCGGTTAAGTATAAGATTTTGGTTTGGTATACGAAAAGAGATCGGGTATGGGGAGGGGCAAATGTGGAAATTGGAGATTCTGGGGAGAAAATGGGAAAGAAAAGTGTGAGGCTTGGAGGGAATGAACCAAGGGTGATAGTGTCCGGCGCATGGAAAGTGTGTGGAGGCTATCGTGCGCTTCTTGTGTTTTGGGTCTTGTTTATCATTGGTCTTGGGTTTACTTGA

Protein sequence

>MS.gene031261.t1

MASSKPNSCCSCCSGFIITIGLMALFIWLSIRVDEPKLYIDKIYLPALNKTLNTTARSNTTITFTLKLVNPNKDKGIQYDPIHLNFTFYNTLNTTLPLGNATVNGFYQGHEKKAKKNGVVEAGVKNLTASVKGVVDGKVYLRVDYFTAVKYKILVWYTKRDRVWGGANVEIGDSGEKMGKKSVRLGGNEPRVIVSGAWKVCGGYRALLVFWVLFIIGLGFT