AlfalfaGEDB | MS.gene87282

M. sativa cultivar XinJiangDaYe / MS.gene87282

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87282.t1	XP_003596635.1	93.2	220	12	1	1	220	1	217	2.70E-111	411.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87282.t1	O48915	46.7	182	90	3	8	185	13	191	6.4e-37	155.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87282.t1	G7IJL6	93.2	220	12	1	1	220	1	217	1.9e-111	411.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049669	MS.gene87282	0.807822	4.19E-50	-1.69E-46
MS.gene050227	MS.gene87282	0.806402	8.37E-50	-1.69E-46
MS.gene050228	MS.gene87282	0.815622	8.41E-52	-1.69E-46
MS.gene052176	MS.gene87282	0.83517	1.96E-56	-1.69E-46
MS.gene052333	MS.gene87282	0.812276	4.60E-51	-1.69E-46
MS.gene052464	MS.gene87282	0.822394	2.43E-53	-1.69E-46
MS.gene052586	MS.gene87282	0.841378	4.93E-58	-1.69E-46
MS.gene053647	MS.gene87282	0.804349	2.25E-49	-1.69E-46
MS.gene054291	MS.gene87282	-0.807485	4.94E-50	-1.69E-46
MS.gene056506	MS.gene87282	0.806808	6.87E-50	-1.69E-46
MS.gene05761	MS.gene87282	0.80406	2.59E-49	-1.69E-46
MS.gene061040	MS.gene87282	-0.856983	2.22E-62	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene99821	MS.gene87282	PPI
MS.gene69812	MS.gene87282	PPI
MS.gene031547	MS.gene87282	PPI
MS.gene87282	MS.gene99821	PPI
MS.gene96823	MS.gene87282	PPI
MS.gene031261	MS.gene87282	PPI
MS.gene87282	MS.gene031261	PPI
MS.gene006756	MS.gene87282	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87282.t1	MTR_2g082990	93.182	220	12	1	1	220	1	217	2.54e-149	414
MS.gene87282.t1	MTR_4g081200	46.698	212	102	5	8	210	11	220	8.19e-58	182

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87282.t1	AT3G20600	42.857	210	112	5	8	213	13	218	3.39e-48	157
MS.gene87282.t1	AT3G20590	43.956	182	94	4	9	185	14	192	1.05e-35	127
MS.gene87282.t1	AT3G20610	35.961	203	116	7	22	217	26	221	9.50e-31	113
MS.gene87282.t1	AT4G05220	22.159	176	122	4	13	180	47	215	3.14e-11	61.2

Find 61 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATACCTTTATCTTTATT+AGG	0.156001	2.2:-14253181	None:intergenic
TGCGGTTAAGTATAAGATTT+TGG	0.193625	2.2:+14253411	MS.gene87282:CDS
TGGAGATTCTGGGGAGAAAA+TGG	0.196345	2.2:+14253483	MS.gene87282:CDS
CTTGGTAATGCTACTGTAAA+TGG	0.197197	2.2:+14253262	MS.gene87282:CDS
CATCATAACCATAGGCTTAA+TGG	0.216575	2.2:+14253018	MS.gene87282:CDS
TGGGTCTTGTTTATTGGTCT+TGG	0.228865	2.2:+14253601	MS.gene87282:CDS
AATGTGGAAATTGGAGATTC+TGG	0.246829	2.2:+14253472	MS.gene87282:CDS
TGATCTTGATGTTGTGTTTA+GGG	0.247761	2.2:-14253122	None:intergenic
GAATACCTTTATCTTTATTA+GGG	0.259706	2.2:-14253180	None:intergenic
ATCGTGCGCTTCTTGTGTTT+TGG	0.270001	2.2:+14253581	MS.gene87282:CDS
TTGATGGTAAAGTTTATCTA+AGG	0.284290	2.2:+14253374	MS.gene87282:CDS
TTGATCTTGATGTTGTGTTT+AGG	0.289617	2.2:-14253123	None:intergenic
GGAGGGGCAAATGTGGAAAT+TGG	0.299189	2.2:+14253463	MS.gene87282:CDS
AAGGTGAAGTTAAGATGAAT+AGG	0.325470	2.2:-14253211	None:intergenic
GGGTCTTGTTTATTGGTCTT+GGG	0.348639	2.2:+14253602	MS.gene87282:CDS
AGCTGTTGTTCATGCTGTTC+AGG	0.351132	2.2:+14252992	MS.gene87282:CDS
AATTTAACTGCGAGTGTTAA+GGG	0.363654	2.2:+14253346	MS.gene87282:CDS
GTTAACCCTAATAAAGATAA+AGG	0.389560	2.2:+14253175	MS.gene87282:CDS
GAATTTAACTGCGAGTGTTA+AGG	0.395995	2.2:+14253345	MS.gene87282:CDS
AGTGTTGAGAGTATTGTAAA+AGG	0.426836	2.2:-14253230	None:intergenic
ACTGTAAATGGATTCTATCA+AGG	0.430123	2.2:+14253274	MS.gene87282:CDS
GGAGATTCTGGGGAGAAAAT+GGG	0.447531	2.2:+14253484	MS.gene87282:CDS
GCTTAATGGCTCTATTCATA+TGG	0.448835	2.2:+14253032	MS.gene87282:CDS
AAAGAGATCGGGTATGGGGA+GGG	0.451284	2.2:+14253446	MS.gene87282:CDS
AGTGTTAAGGGTGTTGTTGA+TGG	0.454047	2.2:+14253358	MS.gene87282:CDS
ATGTGGAAATTGGAGATTCT+GGG	0.462099	2.2:+14253473	MS.gene87282:CDS
AGGTGAAGTTAAGATGAATA+GGG	0.473108	2.2:-14253210	None:intergenic
GGTATGGGGAGGGGCAAATG+TGG	0.473413	2.2:+14253456	MS.gene87282:CDS
AAGAAAAGTGTGAGGCTTGG+TGG	0.478157	2.2:+14253508	MS.gene87282:CDS
CAGCATGAACAACAGCTGTT+GGG	0.497130	2.2:-14252986	None:intergenic
GAATAGAGCCATTAAGCCTA+TGG	0.501191	2.2:-14253026	None:intergenic
ATACGAAAAGAGATCGGGTA+TGG	0.508627	2.2:+14253440	MS.gene87282:CDS
TCCACACACTTTCCATGCGC+CGG	0.510157	2.2:-14253554	None:intergenic
AAGAATGGAGTGGTGGAAGC+TGG	0.512376	2.2:+14253316	MS.gene87282:CDS
AGAAAAGTGTGAGGCTTGGT+GGG	0.514131	2.2:+14253509	MS.gene87282:CDS
GCGCCGGACACTATTACCCT+TGG	0.515021	2.2:-14253538	None:intergenic
GGGTAATAGTGTCCGGCGCA+TGG	0.515594	2.2:+14253542	MS.gene87282:CDS
AAGAGATCGGGTATGGGGAG+GGG	0.523912	2.2:+14253447	MS.gene87282:CDS
AAGTGTGAGGCTTGGTGGGA+AGG	0.527681	2.2:+14253513	MS.gene87282:CDS
GAACCAAGGGTAATAGTGTC+CGG	0.528747	2.2:+14253535	MS.gene87282:CDS
TCAGGTTTCATCATAACCAT+AGG	0.529456	2.2:+14253010	MS.gene87282:CDS
CTCAACACTACTCTTCCACT+TGG	0.532729	2.2:+14253244	MS.gene87282:CDS
TACGAAAAGAGATCGGGTAT+GGG	0.536489	2.2:+14253441	MS.gene87282:CDS
ACAGCATGAACAACAGCTGT+TGG	0.541536	2.2:-14252987	None:intergenic
TTGGTATACGAAAAGAGATC+GGG	0.544628	2.2:+14253435	MS.gene87282:CDS
AGCTAAGAAGAATGGAGTGG+TGG	0.547361	2.2:+14253309	MS.gene87282:CDS
AAAAGAGATCGGGTATGGGG+AGG	0.556071	2.2:+14253445	MS.gene87282:CDS
CTGTAAATGGATTCTATCAA+GGG	0.559809	2.2:+14253275	MS.gene87282:CDS
TTTGGTATACGAAAAGAGAT+CGG	0.560749	2.2:+14253434	MS.gene87282:CDS
TGGTAAAGTTTATCTAAGGG+TGG	0.560905	2.2:+14253378	MS.gene87282:CDS
TGATGGTAAAGTTTATCTAA+GGG	0.567329	2.2:+14253375	MS.gene87282:CDS
GGCTTGGTGGGAAGGAACCA+AGG	0.575803	2.2:+14253521	MS.gene87282:CDS
ACGAAAAGAGATCGGGTATG+GGG	0.597221	2.2:+14253442	MS.gene87282:CDS
GCTTGGTGGGAAGGAACCAA+GGG	0.636656	2.2:+14253522	MS.gene87282:CDS
TGTGGAAATTGGAGATTCTG+GGG	0.648415	2.2:+14253474	MS.gene87282:CDS
GGAAAGAAAAGTGTGAGGCT+TGG	0.648869	2.2:+14253505	MS.gene87282:CDS
GGCGCATGGAAAGTGTGTGG+AGG	0.656040	2.2:+14253556	MS.gene87282:CDS
AAAAGCTAAGAAGAATGGAG+TGG	0.661701	2.2:+14253306	MS.gene87282:CDS
TCCGGCGCATGGAAAGTGTG+TGG	0.671773	2.2:+14253553	MS.gene87282:CDS
TTTACAGTAGCATTACCAAG+TGG	0.674013	2.2:-14253259	None:intergenic
AAATGGGAAAGAAAAGTGTG+AGG	0.693699	2.2:+14253500	MS.gene87282:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAATACCTTTATCTTTATTA+GGG	-	chr2.2:14253183-14253202	None:intergenic	20.0%
!!	TGAATACCTTTATCTTTATT+AGG	-	chr2.2:14253184-14253203	None:intergenic	20.0%
!!!	TTAAGTATAAGATTTTGGTT+TGG	+	chr2.2:14253416-14253435	MS.gene87282:CDS	20.0%
!	GTTAACCCTAATAAAGATAA+AGG	+	chr2.2:14253175-14253194	MS.gene87282:CDS	25.0%
!	TGATGGTAAAGTTTATCTAA+GGG	+	chr2.2:14253375-14253394	MS.gene87282:CDS	25.0%
!!	ATTTTGTCTATGTAAAGCTT+TGG	-	chr2.2:14253079-14253098	None:intergenic	25.0%
!!	TTGATGGTAAAGTTTATCTA+AGG	+	chr2.2:14253374-14253393	MS.gene87282:CDS	25.0%
	AAGGTGAAGTTAAGATGAAT+AGG	-	chr2.2:14253214-14253233	None:intergenic	30.0%
	AATTTAACTGCGAGTGTTAA+GGG	+	chr2.2:14253346-14253365	MS.gene87282:CDS	30.0%
	AGGTGAAGTTAAGATGAATA+GGG	-	chr2.2:14253213-14253232	None:intergenic	30.0%
	GAGAAAAAAGCTAAGAAGAA+TGG	+	chr2.2:14253301-14253320	MS.gene87282:CDS	30.0%
	TGATCTTGATGTTGTGTTTA+GGG	-	chr2.2:14253125-14253144	None:intergenic	30.0%
	TTGATCTTGATGTTGTGTTT+AGG	-	chr2.2:14253126-14253145	None:intergenic	30.0%
	TTTGGTATACGAAAAGAGAT+CGG	+	chr2.2:14253434-14253453	MS.gene87282:CDS	30.0%
!	ACTGTAAATGGATTCTATCA+AGG	+	chr2.2:14253274-14253293	MS.gene87282:CDS	30.0%
!	CTGTAAATGGATTCTATCAA+GGG	+	chr2.2:14253275-14253294	MS.gene87282:CDS	30.0%
!	TGCGGTTAAGTATAAGATTT+TGG	+	chr2.2:14253411-14253430	MS.gene87282:CDS	30.0%
!!	AGTGTTGAGAGTATTGTAAA+AGG	-	chr2.2:14253233-14253252	None:intergenic	30.0%
!!!	TTTTAACGTGAAGTTGATAG+TGG	-	chr2.2:14253152-14253171	None:intergenic	30.0%
	AAAAGCTAAGAAGAATGGAG+TGG	+	chr2.2:14253306-14253325	MS.gene87282:CDS	35.0%
	AATGTGGAAATTGGAGATTC+TGG	+	chr2.2:14253472-14253491	MS.gene87282:CDS	35.0%
	ATGTGGAAATTGGAGATTCT+GGG	+	chr2.2:14253473-14253492	MS.gene87282:CDS	35.0%
	CATCATAACCATAGGCTTAA+TGG	+	chr2.2:14253018-14253037	MS.gene87282:CDS	35.0%
	GAATTTAACTGCGAGTGTTA+AGG	+	chr2.2:14253345-14253364	MS.gene87282:CDS	35.0%
	TCAGGTTTCATCATAACCAT+AGG	+	chr2.2:14253010-14253029	MS.gene87282:CDS	35.0%
	TGGTAAAGTTTATCTAAGGG+TGG	+	chr2.2:14253378-14253397	MS.gene87282:CDS	35.0%
	TTGGTATACGAAAAGAGATC+GGG	+	chr2.2:14253435-14253454	MS.gene87282:CDS	35.0%
	TTTACAGTAGCATTACCAAG+TGG	-	chr2.2:14253262-14253281	None:intergenic	35.0%
!	AAATGGGAAAGAAAAGTGTG+AGG	+	chr2.2:14253500-14253519	MS.gene87282:CDS	35.0%
!	GCTTAATGGCTCTATTCATA+TGG	+	chr2.2:14253032-14253051	MS.gene87282:CDS	35.0%
!!	CTTGGTAATGCTACTGTAAA+TGG	+	chr2.2:14253262-14253281	MS.gene87282:CDS	35.0%
!!!	AAGGGTGGATTATTTTACTG+CGG	+	chr2.2:14253393-14253412	MS.gene87282:CDS	35.0%
!!!	GTGTTTTGGGTCTTGTTTAT+TGG	+	chr2.2:14253595-14253614	MS.gene87282:CDS	35.0%
!!!	TGTGTTTAGGGTTTTGTTGA+GGG	-	chr2.2:14253113-14253132	None:intergenic	35.0%
!!!	TTGTGTTTAGGGTTTTGTTG+AGG	-	chr2.2:14253114-14253133	None:intergenic	35.0%
	ATACGAAAAGAGATCGGGTA+TGG	+	chr2.2:14253440-14253459	MS.gene87282:CDS	40.0%
	GAATAGAGCCATTAAGCCTA+TGG	-	chr2.2:14253029-14253048	None:intergenic	40.0%
	TACGAAAAGAGATCGGGTAT+GGG	+	chr2.2:14253441-14253460	MS.gene87282:CDS	40.0%
	TGTGGAAATTGGAGATTCTG+GGG	+	chr2.2:14253474-14253493	MS.gene87282:CDS	40.0%
!!	AGTGTTAAGGGTGTTGTTGA+TGG	+	chr2.2:14253358-14253377	MS.gene87282:CDS	40.0%
!!	GGGTCTTGTTTATTGGTCTT+GGG	+	chr2.2:14253602-14253621	MS.gene87282:CDS	40.0%
!!	TGGGTCTTGTTTATTGGTCT+TGG	+	chr2.2:14253601-14253620	MS.gene87282:CDS	40.0%
	ACAGCATGAACAACAGCTGT+TGG	-	chr2.2:14252990-14253009	None:intergenic	45.0%
	ACGAAAAGAGATCGGGTATG+GGG	+	chr2.2:14253442-14253461	MS.gene87282:CDS	45.0%
	AGCTAAGAAGAATGGAGTGG+TGG	+	chr2.2:14253309-14253328	MS.gene87282:CDS	45.0%
	AGCTGTTGTTCATGCTGTTC+AGG	+	chr2.2:14252992-14253011	MS.gene87282:CDS	45.0%
	CAGCATGAACAACAGCTGTT+GGG	-	chr2.2:14252989-14253008	None:intergenic	45.0%
	CTCAACACTACTCTTCCACT+TGG	+	chr2.2:14253244-14253263	MS.gene87282:CDS	45.0%
	GAACCAAGGGTAATAGTGTC+CGG	+	chr2.2:14253535-14253554	MS.gene87282:CDS	45.0%
!	AAGAAAAGTGTGAGGCTTGG+TGG	+	chr2.2:14253508-14253527	MS.gene87282:CDS	45.0%
!	AGAAAAGTGTGAGGCTTGGT+GGG	+	chr2.2:14253509-14253528	MS.gene87282:CDS	45.0%
!	ATCGTGCGCTTCTTGTGTTT+TGG	+	chr2.2:14253581-14253600	MS.gene87282:CDS	45.0%
!	GGAAAGAAAAGTGTGAGGCT+TGG	+	chr2.2:14253505-14253524	MS.gene87282:CDS	45.0%
!	GGAGATTCTGGGGAGAAAAT+GGG	+	chr2.2:14253484-14253503	MS.gene87282:CDS	45.0%
!	TCGTGCGCTTCTTGTGTTTT+GGG	+	chr2.2:14253582-14253601	MS.gene87282:CDS	45.0%
!	TGGAGATTCTGGGGAGAAAA+TGG	+	chr2.2:14253483-14253502	MS.gene87282:CDS	45.0%
!!!	TTTAGGGTTTTGTTGAGGGC+AGG	-	chr2.2:14253109-14253128	None:intergenic	45.0%
	AAAAGAGATCGGGTATGGGG+AGG	+	chr2.2:14253445-14253464	MS.gene87282:CDS	50.0%
	AAAGAGATCGGGTATGGGGA+GGG	+	chr2.2:14253446-14253465	MS.gene87282:CDS	50.0%
	GGAGGGGCAAATGTGGAAAT+TGG	+	chr2.2:14253463-14253482	MS.gene87282:CDS	50.0%
!	AAGAATGGAGTGGTGGAAGC+TGG	+	chr2.2:14253316-14253335	MS.gene87282:CDS	50.0%
	AAGAGATCGGGTATGGGGAG+GGG	+	chr2.2:14253447-14253466	MS.gene87282:CDS	55.0%
	TCCACACACTTTCCATGCGC+CGG	-	chr2.2:14253557-14253576	None:intergenic	55.0%
!	GCTTGGTGGGAAGGAACCAA+GGG	+	chr2.2:14253522-14253541	MS.gene87282:CDS	55.0%
!!	AAGTGTGAGGCTTGGTGGGA+AGG	+	chr2.2:14253513-14253532	MS.gene87282:CDS	55.0%
!!!	GGGTTTTGTTGAGGGCAGGA+AGG	-	chr2.2:14253105-14253124	None:intergenic	55.0%
	GCGCCGGACACTATTACCCT+TGG	-	chr2.2:14253541-14253560	None:intergenic	60.0%
	GGGTAATAGTGTCCGGCGCA+TGG	+	chr2.2:14253542-14253561	MS.gene87282:CDS	60.0%
	GGTATGGGGAGGGGCAAATG+TGG	+	chr2.2:14253456-14253475	MS.gene87282:CDS	60.0%
!	GGCGCATGGAAAGTGTGTGG+AGG	+	chr2.2:14253556-14253575	MS.gene87282:CDS	60.0%
!	TCCGGCGCATGGAAAGTGTG+TGG	+	chr2.2:14253553-14253572	MS.gene87282:CDS	60.0%
!!	GGCTTGGTGGGAAGGAACCA+AGG	+	chr2.2:14253521-14253540	MS.gene87282:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	14252971	14253633	14252971	ID=MS.gene87282
chr2.2	mRNA	14252971	14253633	14252971	ID=MS.gene87282.t1;Parent=MS.gene87282
chr2.2	exon	14252971	14253633	14252971	ID=MS.gene87282.t1.exon1;Parent=MS.gene87282.t1
chr2.2	CDS	14252971	14253633	14252971	ID=cds.MS.gene87282.t1;Parent=MS.gene87282.t1

Gene Sequence

>MS.gene87282

ATGGCATCATCAAAACCCAACAGCTGTTGTTCATGCTGTTCAGGTTTCATCATAACCATAGGCTTAATGGCTCTATTCATATGGCTAAGCATACGTGTCGATGAACCAAAGCTTTACATAGACAAAATTTACCTTCCTGCCCTCAACAAAACCCTAAACACAACATCAAGATCAAACACCACTATCAACTTCACGTTAAAACTTGTTAACCCTAATAAAGATAAAGGTATTCAATATGACCCTATTCATCTTAACTTCACCTTTTACAATACTCTCAACACTACTCTTCCACTTGGTAATGCTACTGTAAATGGATTCTATCAAGGGCATGAGAAAAAAGCTAAGAAGAATGGAGTGGTGGAAGCTGGTGTTAAGAATTTAACTGCGAGTGTTAAGGGTGTTGTTGATGGTAAAGTTTATCTAAGGGTGGATTATTTTACTGCGGTTAAGTATAAGATTTTGGTTTGGTATACGAAAAGAGATCGGGTATGGGGAGGGGCAAATGTGGAAATTGGAGATTCTGGGGAGAAAATGGGAAAGAAAAGTGTGAGGCTTGGTGGGAAGGAACCAAGGGTAATAGTGTCCGGCGCATGGAAAGTGTGTGGAGGCTATCGTGCGCTTCTTGTGTTTTGGGTCTTGTTTATTGGTCTTGGGTTTACTTGA

Protein sequence

>MS.gene87282.t1

MASSKPNSCCSCCSGFIITIGLMALFIWLSIRVDEPKLYIDKIYLPALNKTLNTTSRSNTTINFTLKLVNPNKDKGIQYDPIHLNFTFYNTLNTTLPLGNATVNGFYQGHEKKAKKNGVVEAGVKNLTASVKGVVDGKVYLRVDYFTAVKYKILVWYTKRDRVWGGANVEIGDSGEKMGKKSVRLGGKEPRVIVSGAWKVCGGYRALLVFWVLFIGLGFT