Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008697.t1 | NP_001235659.1 | 100 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 3.30E-62 | 247.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008697.t1 | P0DI19 | 99.1 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.1e-64 | 246.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008697.t1 | M5W1X1 | 100.0 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 2.4e-62 | 247.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene002214 | MS.gene008697 | PPI |
| MS.gene008697 | MS.gene75012 | PPI |
| MS.gene029826 | MS.gene008697 | PPI |
| MS.gene008298 | MS.gene008697 | PPI |
| MS.gene048485 | MS.gene008697 | PPI |
| MS.gene53861 | MS.gene008697 | PPI |
| MS.gene05722 | MS.gene008697 | PPI |
| MS.gene008697 | MS.gene53859 | PPI |
| MS.gene008697 | MS.gene24607 | PPI |
| MS.gene01187 | MS.gene008697 | PPI |
| MS.gene071905 | MS.gene008697 | PPI |
| MS.gene048687 | MS.gene008697 | PPI |
| MS.gene008697 | MS.gene04991 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008697.t1 | MTR_3g464510 | 100.000 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 2.57e-77 | 223 |
| MS.gene008697.t1 | MTR_4g022900 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.44e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008697.t1 | AT2G30000 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene008697.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene008697.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene008697.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene008697.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene008697.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
| MS.gene008697.t1 | AT1G07170 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 3.99e-77 | 223 |
Find 28 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCCTAAATTGACAATTT+TGG | 0.090409 | 3.4:+59647928 | None:intergenic |
| TGCCCCAAAATTGTCAATTT+AGG | 0.226054 | 3.4:-59647932 | MS.gene008697:CDS |
| GTGCAACTATGGATCATTTC+AGG | 0.247422 | 3.4:-59648041 | MS.gene008697:CDS |
| TTCATAGAATAAATCAGTTT+TGG | 0.292411 | 3.4:+59647904 | None:intergenic |
| TTCTATGAACGCAAGAAGTA+TGG | 0.313430 | 3.4:-59647890 | MS.gene008697:CDS |
| GCCCCAAAATTGTCAATTTA+GGG | 0.323285 | 3.4:-59647931 | MS.gene008697:CDS |
| GCACACAGCAGGAGAAAGAT+AGG | 0.403394 | 3.4:-59647961 | MS.gene008697:CDS |
| TGCGTCCTTGTACACTTGTA+AGG | 0.414410 | 3.4:-59648075 | MS.gene008697:CDS |
| GTCTGTGATGAGTGCAACTA+TGG | 0.415210 | 3.4:-59648052 | MS.gene008697:CDS |
| AAGCAGCCAGGAATTGCTAT+TGG | 0.443682 | 3.4:-59648148 | MS.gene008697:CDS |
| TAGCAATTCCTGGCTGCTTA+CGG | 0.455501 | 3.4:+59648152 | None:intergenic |
| AATCGTCCAATAGCAATTCC+TGG | 0.467819 | 3.4:+59648142 | None:intergenic |
| TTACGGCACATGATCAAATC+AGG | 0.468198 | 3.4:+59648169 | None:intergenic |
| ATCATGTGCCGTAAGCAGCC+AGG | 0.536811 | 3.4:-59648160 | MS.gene008697:CDS |
| TGCAACTATGGATCATTTCA+GGG | 0.537300 | 3.4:-59648040 | MS.gene008697:CDS |
| CAGACCCTTACAAGTGTACA+AGG | 0.539307 | 3.4:+59648070 | None:intergenic |
| CAGGGTCGATGCGTCATATG+TGG | 0.556039 | 3.4:-59648022 | MS.gene008697:CDS |
| CTGCAAGGAATGCACACAGC+AGG | 0.557725 | 3.4:-59647972 | MS.gene008697:CDS |
| GATCAAATCAGGATGATGCT+TGG | 0.567091 | 3.4:+59648180 | None:intergenic |
| TTCCTTGCAGTAGTAAGCAT+CGG | 0.596378 | 3.4:+59647985 | None:intergenic |
| ATCCGATGCTTACTACTGCA+AGG | 0.604803 | 3.4:-59647987 | MS.gene008697:CDS |
| GCGTCCTTGTACACTTGTAA+GGG | 0.606045 | 3.4:-59648074 | MS.gene008697:CDS |
| TGCGTCATATGTGGAGGAGT+AGG | 0.606828 | 3.4:-59648013 | MS.gene008697:CDS |
| CACACAGCAGGAGAAAGATA+GGG | 0.609822 | 3.4:-59647960 | MS.gene008697:CDS |
| AAGTGTACAAGGACGCACAT+AGG | 0.637033 | 3.4:+59648081 | None:intergenic |
| GGTCGATGCGTCATATGTGG+AGG | 0.657559 | 3.4:-59648019 | MS.gene008697:CDS |
| CGATTATGCGAGAAATGTGA+CGG | 0.661791 | 3.4:-59648124 | MS.gene008697:CDS |
| CAGCAGGAGAAAGATAGGGA+TGG | 0.676444 | 3.4:-59647956 | MS.gene008697:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATAGAATAAATCAGTTT+TGG | + | chr3.4:59648157-59648176 | None:intergenic | 20.0% |
| ! | ACTCCCTAAATTGACAATTT+TGG | + | chr3.4:59648133-59648152 | None:intergenic | 30.0% |
| ! | CTCCCTAAATTGACAATTTT+GGG | + | chr3.4:59648132-59648151 | None:intergenic | 30.0% |
| ! | TCCCTAAATTGACAATTTTG+GGG | + | chr3.4:59648131-59648150 | None:intergenic | 30.0% |
| GCCCCAAAATTGTCAATTTA+GGG | - | chr3.4:59648127-59648146 | MS.gene008697:CDS | 35.0% | |
| TGCAACTATGGATCATTTCA+GGG | - | chr3.4:59648018-59648037 | MS.gene008697:CDS | 35.0% | |
| TGCCCCAAAATTGTCAATTT+AGG | - | chr3.4:59648126-59648145 | MS.gene008697:CDS | 35.0% | |
| TTCTATGAACGCAAGAAGTA+TGG | - | chr3.4:59648168-59648187 | MS.gene008697:CDS | 35.0% | |
| AATCGTCCAATAGCAATTCC+TGG | + | chr3.4:59647919-59647938 | None:intergenic | 40.0% | |
| CGATTATGCGAGAAATGTGA+CGG | - | chr3.4:59647934-59647953 | MS.gene008697:CDS | 40.0% | |
| GATCAAATCAGGATGATGCT+TGG | + | chr3.4:59647881-59647900 | None:intergenic | 40.0% | |
| GTGCAACTATGGATCATTTC+AGG | - | chr3.4:59648017-59648036 | MS.gene008697:CDS | 40.0% | |
| TTACGGCACATGATCAAATC+AGG | + | chr3.4:59647892-59647911 | None:intergenic | 40.0% | |
| TTCCTTGCAGTAGTAAGCAT+CGG | + | chr3.4:59648076-59648095 | None:intergenic | 40.0% | |
| AAGCAGCCAGGAATTGCTAT+TGG | - | chr3.4:59647910-59647929 | MS.gene008697:CDS | 45.0% | |
| AAGTGTACAAGGACGCACAT+AGG | + | chr3.4:59647980-59647999 | None:intergenic | 45.0% | |
| ATCCGATGCTTACTACTGCA+AGG | - | chr3.4:59648071-59648090 | MS.gene008697:CDS | 45.0% | |
| CACACAGCAGGAGAAAGATA+GGG | - | chr3.4:59648098-59648117 | MS.gene008697:CDS | 45.0% | |
| CAGACCCTTACAAGTGTACA+AGG | + | chr3.4:59647991-59648010 | None:intergenic | 45.0% | |
| GCGTCCTTGTACACTTGTAA+GGG | - | chr3.4:59647984-59648003 | MS.gene008697:CDS | 45.0% | |
| GTCTGTGATGAGTGCAACTA+TGG | - | chr3.4:59648006-59648025 | MS.gene008697:CDS | 45.0% | |
| TAGCAATTCCTGGCTGCTTA+CGG | + | chr3.4:59647909-59647928 | None:intergenic | 45.0% | |
| TGCGTCCTTGTACACTTGTA+AGG | - | chr3.4:59647983-59648002 | MS.gene008697:CDS | 45.0% | |
| CAGCAGGAGAAAGATAGGGA+TGG | - | chr3.4:59648102-59648121 | MS.gene008697:CDS | 50.0% | |
| GCACACAGCAGGAGAAAGAT+AGG | - | chr3.4:59648097-59648116 | MS.gene008697:CDS | 50.0% | |
| TGCGTCATATGTGGAGGAGT+AGG | - | chr3.4:59648045-59648064 | MS.gene008697:CDS | 50.0% | |
| ATCATGTGCCGTAAGCAGCC+AGG | - | chr3.4:59647898-59647917 | MS.gene008697:CDS | 55.0% | |
| CAGGGTCGATGCGTCATATG+TGG | - | chr3.4:59648036-59648055 | MS.gene008697:CDS | 55.0% | |
| CTGCAAGGAATGCACACAGC+AGG | - | chr3.4:59648086-59648105 | MS.gene008697:CDS | 55.0% | |
| GGTCGATGCGTCATATGTGG+AGG | - | chr3.4:59648039-59648058 | MS.gene008697:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 59647874 | 59648206 | 59647874 | ID=MS.gene008697 |
| chr3.4 | mRNA | 59647874 | 59648206 | 59647874 | ID=MS.gene008697.t1;Parent=MS.gene008697 |
| chr3.4 | exon | 59647874 | 59648206 | 59647874 | ID=MS.gene008697.t1.exon1;Parent=MS.gene008697.t1 |
| chr3.4 | CDS | 59647874 | 59648206 | 59647874 | ID=cds.MS.gene008697.t1;Parent=MS.gene008697.t1 |
| Gene Sequence |
| Protein sequence |
